Rh6DG368200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000978
Physical Location & Seq
Reverse (-)
56437028 .. 56439271
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG368200.1

Sequence Viewer

Length: 1134 bp
ATGCTTCTATTGCATATTACCAGTATTACTGTAAGCCCTGCCTTGGCTTTGGAAGACTGCGAGTTTCCAGCCATCTTCAACTTCGGAGACTCAAATTCGGATACTGGTGGATTCGCAGCATCCATTTATCCCCCACCTACACCTTATGGAGAGACCTATTTTCATATGCCGGTTGGAAGAATCTCAGATGGAAGACTCATTGTCGATTTCCTCGCAACGAGTCTTGGTCATCCTTATCTAAGCGCATATCTGGATTCCCTCGGGACCAATTTCTCGCATGGTGCAAATTTTGCCACTTCAGCTTCCACTATCAGACTCCCAGATCTCATTTTACCAGCCGGAGGATTTAGTCCCTTCTACCTTGATATTCAATACGTGCAGTTCCGGGAACTAAAGTCCAGATCACAACTCATAAGGCAACGAGGGGGAATATTTGCAAGTTTAATGCCCAAGGAGGAGTACTTCTCCAAAGCTTTATACACATTCGACATTGGTCAGAATGATCTTGGCGAGGGATTTTTCGGTAACAAGACTATACAGGAAGTCAATGCATCTGTTCCTGATATAATCACTGGTTTCTCAACTAATATTAAGAAAATATATGATTTGGGGGCAAGATCATTTTGGATCCACAATACGGGGCCGATTGGCTGTCTCCCTTACATTTTGGTGAAATTCCCATCAGCTCAGAAAGATGAGGTTGGCTGTGCAAAGGCTTACAATGAAGTAGCTCAACATTTTAATCACGAGCTGAAGCAGGCCATAGTTCAACAACTCAGGAAGGATCTTCCTTTGGCTGCATTCACTTATGTAGATGTGTATTCCGTCAAATATTCTATTTTCAAGGAACCCAAAAAATATGGATTTGAGCTCCCACTTGTTGCTTGTTGTGGCTATGGTGGTAAGTACAACTATAACAGTAGTACAGGATGTGGAACAACAATCACAGTCAATGGAAGTCAAATCTTTGTTGGCTCATGCAAGAACCCATCAGCTAGAGTGAACTGGGATGGGATTCATTATACTGAGGCAGCTGCGAAATTTGTTTTTGAGAAAATCTCAACTGGAGCATATTCAGATCCACCTCTGCCATTGAAACAAGCATGCCACAGGAGTGGAAATAAGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

377

Amino Acids

41.5

Weight (kDa)

8.01

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 25 - 353 4.4e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 622, 635, 792, 1073
AcsI RAATTY 4 cut(s) 94, 286, 674, 1040
AcuI CTGAAG 2 cut(s) 282, 773
AfaI GTAC 3 cut(s) 461, 908, 925
AfiI CCNNNNNNNGG 3 cut(s) 43, 341, 637
AgsI TTSAA 5 cut(s) 79, 371, 770, 844, 1096
AhdI GACNNNNNGTC 1 cut(s) 200
AleI CACNNNNGTG 1 cut(s) 1113
AluBI AGCT 8 cut(s) 302, 473, 686, 731, 751, 871, 995, 1034
AluI AGCT 8 cut(s) 302, 473, 686, 731, 751, 871, 995, 1034
Alw21I GWGCWC 1 cut(s) 873
Alw26I GTCTC 3 cut(s) 81, 146, 659
AlwI GGATC 4 cut(s) 622, 635, 792, 1073
Ama87I CYCGRG 1 cut(s) 260
AoxI GGCC 2 cut(s) 641, 759
ApeKI GCWGC 4 cut(s) 116, 797, 1031, 1034
ApoI RAATTY 4 cut(s) 94, 286, 674, 1040
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 264, 641
AsuC2I CCSGG 1 cut(s) 386
AsuHPI GGTGA 1 cut(s) 682
AvaI CYCGRG 1 cut(s) 260
AvaII GGWCC 1 cut(s) 264
BamHI GGATCC 1 cut(s) 627
BanII GRGCYC 1 cut(s) 873
BauI CACGAG 1 cut(s) 746
BbsI GAAGAC 2 cut(s) 60, 199
Bbv12I GWGCWC 1 cut(s) 873
BbvI GCAGC 4 cut(s) 128, 784, 1021, 1043
BccI CCATC 5 cut(s) 80, 182, 688, 997, 1004
BciVI GTATCC 1 cut(s) 94
BcnI CCSGG 1 cut(s) 386
BcoDI GTCTC 3 cut(s) 81, 146, 659
BfaI CTAG 1 cut(s) 996
BfuI GTATCC 1 cut(s) 94
BglII AGATCT 1 cut(s) 322
BisI GCNGC 4 cut(s) 117, 798, 1032, 1035
BlsI GCNGC 4 cut(s) 118, 799, 1033, 1036
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 264
BmeRI GACNNNNNGTC 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 264, 641
BmiI GGNNCC 4 cut(s) 265, 629, 642, 849
BmrFI CCNGG 1 cut(s) 386
BmrI ACTGGG 1 cut(s) 1015
BmsI GCATC 2 cut(s) 128, 560
BmuI ACTGGG 1 cut(s) 1015
BoxI GACNNNNGTC 1 cut(s) 492
BpiI GAAGAC 2 cut(s) 60, 199
BplI GAGNNNNNCTC 4 cut(s) 449, 481, 1043, 1075
BpmI CTGGAG 1 cut(s) 1086
BpuMI CCSGG 1 cut(s) 386
BsaAI YACGTR 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 3 cut(s) 42, 259, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 341, 637
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 5 cut(s) 21, 109, 577, 1010, 1069
BseDI CCNNGG 3 cut(s) 42, 259, 450
BseGI GGATG 4 cut(s) 119, 229, 935, 1015
BseLI CCNNNNNNNGG 3 cut(s) 43, 341, 637
BseMII CTCAG 4 cut(s) 198, 701, 790, 1017
BseNI ACTGG 5 cut(s) 21, 109, 577, 1010, 1069
BseRI GAGGAG 1 cut(s) 470
BseXI GCAGC 4 cut(s) 128, 784, 1021, 1043
BsgI GTGCAG 1 cut(s) 398
BshFI GGCC 2 cut(s) 643, 761
BsiHKAI GWGCWC 1 cut(s) 873
BsiHKCI CYCGRG 1 cut(s) 260
BsiSI CCGG 3 cut(s) 170, 339, 385
BslFI GGGAC 2 cut(s) 277, 336
BslI CCNNNNNNNGG 3 cut(s) 43, 341, 637
BsmAI GTCTC 3 cut(s) 81, 146, 659
BsmFI GGGAC 2 cut(s) 277, 336
BsmI GAATGC 1 cut(s) 800
BsnI GGCC 2 cut(s) 643, 761
Bso31I GGTCTC 1 cut(s) 146
BsoBI CYCGRG 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 873
Bsp143I GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
BspANI GGCC 2 cut(s) 643, 761
BspCNI CTCAG 4 cut(s) 197, 700, 789, 1018
BspLI GGNNCC 4 cut(s) 265, 629, 642, 849
BspPI GGATC 4 cut(s) 622, 635, 792, 1073
BspTNI GGTCTC 1 cut(s) 146
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 5 cut(s) 21, 109, 577, 1010, 1069
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 42, 259, 450
BssMI GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
BssSI CACGAG 1 cut(s) 746
BssT1I CCWWGG 2 cut(s) 42, 450
Bst2BI CACGAG 1 cut(s) 746
Bst4CI ACNGT 3 cut(s) 31, 920, 949
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBAI YACGTR 1 cut(s) 376
BstC8I GCNNGC 2 cut(s) 759, 1105
BstDEI CTNAG 5 cut(s) 184, 239, 687, 776, 1026
BstF5I GGATG 4 cut(s) 119, 229, 935, 1015
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 7 cut(s) 325, 404, 505, 620, 630, 787, 1081
BstMAI GTCTC 3 cut(s) 81, 146, 659
BstMBI GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
BstMWI GCNNNNNNNGC 3 cut(s) 10, 290, 299
BstNSI RCATGY 1 cut(s) 1107
BstPAI GACNNNNGTC 1 cut(s) 492
BstSCI CCNGG 1 cut(s) 384
BstV1I GCAGC 4 cut(s) 128, 784, 1021, 1043
BstV2I GAAGAC 2 cut(s) 60, 199
BstX2I RGATCY 4 cut(s) 322, 627, 784, 1078
BstXI CCANNNNNNTGG 1 cut(s) 1115
BstYI RGATCY 4 cut(s) 322, 627, 784, 1078
BsuI GTATCC 1 cut(s) 94
BsuRI GGCC 2 cut(s) 643, 761
BtsCI GGATG 4 cut(s) 119, 229, 935, 1015
BtsIMutI CAGTG 1 cut(s) 570
Cac8I GCNNGC 2 cut(s) 759, 1105
CfoI GCGC 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 264, 641
Csp6I GTAC 3 cut(s) 460, 907, 924
CviAII CATG 3 cut(s) 278, 978, 1104
CviQI GTAC 3 cut(s) 460, 907, 924
DdeI CTNAG 5 cut(s) 184, 239, 687, 776, 1026
DpnI GATC 7 cut(s) 324, 403, 504, 619, 629, 786, 1080
DpnII GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
DriI GACNNNNNGTC 1 cut(s) 200
Eam1105I GACNNNNNGTC 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 871
Eco130I CCWWGG 2 cut(s) 42, 450
Eco24I GRGCYC 1 cut(s) 873
Eco31I GGTCTC 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 871
Eco57I CTGAAG 2 cut(s) 282, 773
Eco88I CYCGRG 1 cut(s) 260
EcoICRI GAGCTC 1 cut(s) 871
EcoT14I CCWWGG 2 cut(s) 42, 450
EcoT22I ATGCAT 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 873
ErhI CCWWGG 2 cut(s) 42, 450
FaeI CATG 3 cut(s) 281, 981, 1107
FaqI GGGAC 2 cut(s) 277, 336
FatI CATG 3 cut(s) 277, 977, 1103
FauNDI CATATG 1 cut(s) 165
Fnu4HI GCNGC 4 cut(s) 117, 798, 1032, 1035
FokI GGATG 4 cut(s) 106, 216, 942, 1022
FriOI GRGCYC 1 cut(s) 873
Fsp4HI GCNGC 4 cut(s) 117, 798, 1032, 1035
FspBI CTAG 1 cut(s) 996
GlaI GCGC 1 cut(s) 244
GluI GCNGC 4 cut(s) 117, 798, 1032, 1035
GsuI CTGGAG 1 cut(s) 1086
HaeIII GGCC 2 cut(s) 643, 761
HapII CCGG 3 cut(s) 170, 339, 385
HhaI GCGC 1 cut(s) 245
Hin1II CATG 3 cut(s) 281, 981, 1107
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HindIII AAGCTT 1 cut(s) 471
HinfI GANTC 8 cut(s) 89, 111, 180, 195, 220, 254, 315, 1015
HpaII CCGG 3 cut(s) 170, 339, 385
HphI GGTGA 1 cut(s) 682
Hpy166II GTNNAC 1 cut(s) 1003
Hpy188I TCNGA 7 cut(s) 86, 100, 187, 314, 498, 690, 1078
Hpy188III TCNNGA 6 cut(s) 251, 262, 399, 560, 746, 778
Hpy8I GTNNAC 1 cut(s) 1003
HpyAV CCTTC 2 cut(s) 364, 775
HpyCH4III ACNGT 3 cut(s) 31, 920, 949
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 8 cut(s) 13, 284, 379, 437, 551, 710, 800, 981
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 290, 299
HpyF3I CTNAG 5 cut(s) 184, 239, 687, 776, 1026
HpySE526I ACGT 1 cut(s) 375
Hsp92II CATG 3 cut(s) 281, 981, 1107
HspAI GCGC 1 cut(s) 243
Kzo9I GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
LmnI GCTCC 2 cut(s) 876, 1067
Lsp1109I GCAGC 4 cut(s) 128, 784, 1021, 1043
LweI GCATC 2 cut(s) 128, 560
MaeI CTAG 1 cut(s) 996
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 524
MalI GATC 7 cut(s) 324, 403, 504, 619, 629, 786, 1080
MboI GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
MboII GAAGA 5 cut(s) 65, 67, 189, 204, 779
MflI RGATCY 4 cut(s) 322, 627, 784, 1078
MhlI GDGCHC 1 cut(s) 873
MluCI AATT 5 cut(s) 94, 268, 286, 674, 1040
MlyI GAGTC 4 cut(s) 83, 189, 229, 309
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 9 cut(s) 221, 269, 335, 416, 448, 505, 691, 1021, 1095
Mph1103I ATGCAT 1 cut(s) 553
MseI TTAA 3 cut(s) 443, 591, 741
MslI CAYNNNNRTG 2 cut(s) 668, 1113
MspA1I CMGCKG 1 cut(s) 1034
MspI CCGG 3 cut(s) 170, 339, 385
MspR9I CCNGG 1 cut(s) 386
Mva1269I GAATGC 1 cut(s) 800
MwoI GCNNNNNNNGC 3 cut(s) 10, 290, 299
NciI CCSGG 1 cut(s) 386
NdeI CATATG 1 cut(s) 165
NdeII GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
NlaIII CATG 3 cut(s) 281, 981, 1107
NlaIV GGNNCC 4 cut(s) 265, 629, 642, 849
NsiI ATGCAT 1 cut(s) 553
NspI RCATGY 1 cut(s) 1107
OliI CACNNNNGTG 1 cut(s) 1113
PaeI GCATGC 1 cut(s) 1107
PctI GAATGC 1 cut(s) 800
PfeI GAWTC 4 cut(s) 111, 180, 254, 1015
PfoI TCCNGGA 1 cut(s) 384
PkrI GCNGC 4 cut(s) 118, 799, 1033, 1036
PleI GAGTC 4 cut(s) 83, 189, 228, 309
PpsI GAGTC 4 cut(s) 83, 189, 228, 309
Ppu21I YACGTR 1 cut(s) 376
PshAI GACNNNNGTC 1 cut(s) 492
Psp124BI GAGCTC 1 cut(s) 873
PspN4I GGNNCC 4 cut(s) 265, 629, 642, 849
PspPI GGNCC 2 cut(s) 264, 641
PsuI RGATCY 4 cut(s) 322, 627, 784, 1078
PvuII CAGCTG 1 cut(s) 1034
RsaI GTAC 3 cut(s) 461, 908, 925
RsaNI GTAC 3 cut(s) 460, 907, 924
RseI CAYNNNNRTG 2 cut(s) 668, 1113
SacI GAGCTC 1 cut(s) 873
SaqAI TTAA 3 cut(s) 443, 591, 741
SatI GCNGC 4 cut(s) 117, 798, 1032, 1035
Sau3AI GATC 7 cut(s) 322, 401, 502, 617, 627, 784, 1078
Sau96I GGNCC 2 cut(s) 264, 641
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 4 cut(s) 83, 189, 229, 309
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 1 cut(s) 873
SfaNI GCATC 2 cut(s) 128, 560
SinI GGWCC 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 668, 1113
SphI GCATGC 1 cut(s) 1107
Sse9I AATT 5 cut(s) 94, 268, 286, 674, 1040
SspI AATATT 3 cut(s) 432, 589, 833
SspMI CTAG 1 cut(s) 996
SstI GAGCTC 1 cut(s) 873
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 2 cut(s) 42, 450
TaaI ACNGT 3 cut(s) 31, 920, 949
TaiI ACGT 1 cut(s) 378
TaqI TCGA 2 cut(s) 204, 486
TasI AATT 5 cut(s) 94, 268, 286, 674, 1040
TatI WGTACW 3 cut(s) 459, 906, 923
TfiI GAWTC 4 cut(s) 111, 180, 254, 1015
Tru1I TTAA 3 cut(s) 443, 591, 741
Tru9I TTAA 3 cut(s) 443, 591, 741
TscAI CASTG 1 cut(s) 577
TseI GCWGC 4 cut(s) 116, 797, 1031, 1034
TspDTI ATGAA 3 cut(s) 152, 738, 1007
TspGWI ACGGA 1 cut(s) 814
TspRI CASTG 1 cut(s) 577
VpaK11BI GGWCC 1 cut(s) 264
XapI RAATTY 4 cut(s) 94, 286, 674, 1040
XceI RCATGY 1 cut(s) 1107
XspI CTAG 1 cut(s) 996
ZrmI AGTACT 1 cut(s) 461
Zsp2I ATGCAT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.