MD15G1348900.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000978
Physical Location & Seq
Forward (+)
41345118 .. 41347925
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1348900.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGAGTCACCGATAATTACTATCAAAGTTATTTCATTCTTTTGCTCGTACATGCTTTTGTCGCACTTGCATCTCACCACTTTGAACCCTACTGCCTTTGCTTTGGAGGACTGTCAGTTTCCAGCCGTCTTTAACTTTGGGGACTCTAATTCAGATACTGGCGGATTGGCCTCATCCCTTATTGCACCAACCCCTCCTTATGGTGAGACATTTTTTGGTATGCCGGCCGGAAGATTCTCAGATGGCCGTCTCACAATCGATTTCCTCGCAAAAAGTGTTGGCCACCCTTTTCTAAGCGCATATTTGGATTCACTTGGGACCAACTTCTCTTCCGGTGCAAATTTTGCCACTGCAGCTTCCACAAATCAGGCCTGCCCAATAGCGTTACTTACAAAGTGGTGGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005488 GO:0006950 GO:0006952 GO:0008150 GO:0016787 GO:0016788 GO:0019863 GO:0019865 GO:0044877 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

136

Amino Acids

14.48

Weight (kDa)

5.0

Isoelectric Point (pI)

29.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 124 6.8e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 162
AcoI YGGCCR 3 cut(s) 225, 244, 280
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 1 cut(s) 85
AluBI AGCT 1 cut(s) 356
AluI AGCT 1 cut(s) 356
Alw26I GTCTC 2 cut(s) 200, 254
AlwNI CAGNNNCTG 1 cut(s) 158
AoxI GGCC 5 cut(s) 168, 225, 244, 280, 369
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 340
AspLEI GCGC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 67, 215
AvaII GGWCC 1 cut(s) 318
BalI TGGCCA 1 cut(s) 282
BbvI GCAGC 1 cut(s) 365
BccI CCATC 1 cut(s) 236
BceAI ACGGC 2 cut(s) 110, 231
BcoDI GTCTC 2 cut(s) 200, 254
BfmI CTRYAG 1 cut(s) 351
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme18I GGWCC 1 cut(s) 318
BmgT120I GGNCC 1 cut(s) 318
BmiI GGNNCC 1 cut(s) 319
BmsI GCATC 1 cut(s) 79
Bsa29I ATCGAT 1 cut(s) 258
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 163
BseCI ATCGAT 1 cut(s) 258
BseGI GGATG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 163
BseX3I CGGCCG 1 cut(s) 225
BseXI GCAGC 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 5 cut(s) 170, 227, 246, 282, 371
BshVI ATCGAT 1 cut(s) 258
BsiEI CGRYCG 1 cut(s) 228
BsiSI CCGG 3 cut(s) 224, 228, 333
BslFI GGGAC 2 cut(s) 155, 331
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 2 cut(s) 200, 254
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 2 cut(s) 155, 331
BsnI GGCC 5 cut(s) 170, 227, 246, 282, 371
BspACI CCGC 1 cut(s) 162
BspANI GGCC 5 cut(s) 170, 227, 246, 282, 371
BspCNI CTCAG 1 cut(s) 251
BspDI ATCGAT 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 319
BspMAI CTGCAG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 223
BsrI ACTGG 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 223
Bst4CI ACNGT 1 cut(s) 113
Bst6I CTCTTC 1 cut(s) 334
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 225, 373
BstDEI CTNAG 2 cut(s) 238, 293
BstF5I GGATG 1 cut(s) 173
BstHHI GCGC 1 cut(s) 299
BstMAI GTCTC 2 cut(s) 200, 254
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 3 cut(s) 61, 344, 353
BstNSI RCATGY 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 365
BstZI CGGCCG 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 258
BsuRI GGCC 5 cut(s) 170, 227, 246, 282, 371
BsuTUI ATCGAT 1 cut(s) 258
BtsCI GGATG 1 cut(s) 173
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 225, 373
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 318
ClaI ATCGAT 1 cut(s) 258
Csp6I GTAC 1 cut(s) 49
CviAII CATG 1 cut(s) 52
CviJI RGCY 7 cut(s) 125, 170, 227, 246, 282, 356, 371
CviKI_1 RGCY 7 cut(s) 125, 170, 227, 246, 282, 356, 371
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 2 cut(s) 238, 293
EaeI YGGCCR 3 cut(s) 225, 244, 280
EagI CGGCCG 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 334
EarI CTCTTC 1 cut(s) 334
EciI GGCGGA 1 cut(s) 177
EclXI CGGCCG 1 cut(s) 225
Eco147I AGGCCT 1 cut(s) 371
Eco47I GGWCC 1 cut(s) 318
Eco52I CGGCCG 1 cut(s) 225
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 1 cut(s) 55
FaiI YATR 4 cut(s) 53, 201, 221, 301
FaqI GGGAC 2 cut(s) 155, 331
FatI CATG 1 cut(s) 51
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 1 cut(s) 160
Fsp4HI GCNGC 1 cut(s) 354
GlaI GCGC 1 cut(s) 298
GluI GCNGC 1 cut(s) 354
HaeIII GGCC 5 cut(s) 170, 227, 246, 282, 371
HapII CCGG 3 cut(s) 224, 228, 333
HhaI GCGC 1 cut(s) 299
Hin1II CATG 1 cut(s) 55
Hin6I GCGC 1 cut(s) 297
HinP1I GCGC 1 cut(s) 297
HinfI GANTC 4 cut(s) 5, 143, 234, 308
HpaII CCGG 3 cut(s) 224, 228, 333
HphI GGTGA 2 cut(s) 67, 215
Hpy188I TCNGA 2 cut(s) 154, 241
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4V TGCA 4 cut(s) 70, 185, 338, 353
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 344, 353
HpyF3I CTNAG 2 cut(s) 238, 293
Hsp92II CATG 1 cut(s) 55
HspAI GCGC 1 cut(s) 297
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
LpnPI CCDG 7 cut(s) 135, 144, 237, 241, 346, 353, 385
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 1 cut(s) 79
MaeIII GTNAC 2 cut(s) 6, 384
MboII GAAGA 2 cut(s) 243, 321
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 3 cut(s) 15, 148, 340
MluNI TGGCCA 1 cut(s) 282
MlyI GAGTC 2 cut(s) 14, 137
MnlI CCTC 4 cut(s) 100, 181, 204, 275
Mox20I TGGCCA 1 cut(s) 282
MroNI GCCGGC 1 cut(s) 223
MscI TGGCCA 1 cut(s) 282
MseI TTAA 1 cut(s) 132
Msp20I TGGCCA 1 cut(s) 282
MspI CCGG 3 cut(s) 224, 228, 333
MwoI GCNNNNNNNGC 3 cut(s) 61, 344, 353
NaeI GCCGGC 1 cut(s) 225
NgoMIV GCCGGC 1 cut(s) 223
NlaIII CATG 1 cut(s) 55
NlaIV GGNNCC 1 cut(s) 319
NmuCI GTSAC 1 cut(s) 6
NspI RCATGY 1 cut(s) 55
PceI AGGCCT 1 cut(s) 371
PdiI GCCGGC 1 cut(s) 225
PfeI GAWTC 2 cut(s) 234, 308
PkrI GCNGC 1 cut(s) 355
PleI GAGTC 2 cut(s) 13, 137
PpsI GAGTC 2 cut(s) 13, 137
PspN4I GGNNCC 1 cut(s) 319
PspPI GGNCC 1 cut(s) 318
PstI CTGCAG 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 158
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 1 cut(s) 354
Sau96I GGNCC 1 cut(s) 318
SchI GAGTC 2 cut(s) 14, 137
SetI ASST 1 cut(s) 358
SfaNI GCATC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 351
SinI GGWCC 1 cut(s) 318
Sse9I AATT 3 cut(s) 15, 148, 340
SseBI AGGCCT 1 cut(s) 371
SsiI CCGC 1 cut(s) 162
StuI AGGCCT 1 cut(s) 371
TaaI ACNGT 1 cut(s) 113
TaqI TCGA 1 cut(s) 258
TasI AATT 3 cut(s) 15, 148, 340
TfiI GAWTC 2 cut(s) 234, 308
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 1 cut(s) 355
TseFI GTSAC 1 cut(s) 6
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 1 cut(s) 24
TspRI CASTG 1 cut(s) 355
VpaK11BI GGWCC 1 cut(s) 318
XapI RAATTY 1 cut(s) 340
XceI RCATGY 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.