FvH4_2g31520

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000799
Physical Location & Seq
Forward (+)
24142871 .. 24146267
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31520.t1

Sequence Viewer

Length: 1242 bp
ATGGCTGGCGCTGCACCGGAAGGGTCTCAGTTTGATACTCGTCAATACGATGCCAAAATGACCGAGCTACTTGGATCTGATGGACAAGATTTCTTCACCTCGTATGACGAGGTTCATGACAGTTTTGATGTCATGGGACTGCAGGAAAACCTTCTTAGGGGAATCTATGCATATGGTTTTGAGAAGCCCTCTGCAATTCAGCAAAGGGGAATTGTTCCCTTCTGCAAGGGACTTGATGTTATCCAACAGGCACAATCTGGAACTGGAAAAACAGCCACTTTCTGCTCTGGAATTTTGCAGCAGCTTGATTATGCCATGCTCGACTGCCAGGCATTAGTTCTTGCACCTACCCGAGAACTTGCTCAACAGATTGAGAAGGTTATGCGGGCTCTCGGTGACTACCTCGGTGTCAAGGTTCATGCTTGTGTTGGTGGGACTAGTGTCCGTGAAGATCAACGTGTTCTCTCTAGCGGAGTCCATGTTGTTGTTGGTACACCTGGCCGAGTCTTTGACATGTTGCGGAGGCAGTCTCTCCGTCCAGATTGCATTAGGATGTTTGTGTTAGATGAAGCTGATGAAATGCTCTCAAGAGGTTTCAAGGATCAGATCTATGACATTTTCCAGCTGCTCCCACCCAAAATTCAGGTGGGTGTTTTTTCTGCCACAATGCCACCAGAAGCCCTTGATATCACCAGGAAGTTCATGAACAAGCCTGTGAGAATTTTGGTGAAACGTGATGAGCTCACTCTTGAGGGTATTAAGCAGTTCCATGTCAATGTTGACAAGGAAGAGTGGAAGCTTGAAACGCTTTGCGATCTTTATGAGACCTTGGCAATCACCCAGAGTGTCATCTTTGTGAACACCAGGCGCAAAGTTGATTGGCTGACAGACAAGATGCGCAGCCGTGATCACACGGTCTCTGCCACCCATGGTGACATGGAGCAAAACACTAGGGACATCATCATGAGAGAGTTTAGGTCTGGTTCTTCTCGTGTGCTCATCACAACTGATCTGTTGGCCCGTGGTATTGATGTCCAGCAGGTGTCTCTTGTGATTAACTATGACCTTCCTACACAGCCGGAAAACTACCTGCATCGTATTGGTCGTAGTGGTAGGTTTGGGAGGAAGGGTGTTGCCATCAACTTTGTGACCAAGGATGATGAAAGGATGTTGTTTGACATCCAGAAGTTCTACAATGTCGTAATTGAGGAGCTGCCTGCTAATGTTGCTGATCTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

414

Amino Acids

46.74

Weight (kDa)

5.53

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 65 - 227 1.8e-42 DEAD/DEAH box helicase
ResIII PF04851 83 - 223 3.3e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 266 - 374 6.8e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1030
Acc16I TGCGCA 1 cut(s) 899
Acc36I ACCTGC 2 cut(s) 1030, 1098
AciI CCGC 3 cut(s) 385, 471, 520
AclWI GGATC 2 cut(s) 82, 609
AcoI YGGCCR 1 cut(s) 499
AcsI RAATTY 3 cut(s) 291, 639, 720
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 1 cut(s) 157
AflIII ACRYGT 2 cut(s) 457, 513
AgsI TTSAA 2 cut(s) 598, 803
AhlI ACTAGT 1 cut(s) 437
AjnI CCWGG 4 cut(s) 327, 496, 692, 863
AluBI AGCT 7 cut(s) 67, 304, 572, 625, 742, 799, 1213
AluI AGCT 7 cut(s) 67, 304, 572, 625, 742, 799, 1213
Alw21I GWGCWC 2 cut(s) 744, 999
Alw26I GTCTC 5 cut(s) 30, 534, 818, 922, 1050
AlwI GGATC 2 cut(s) 82, 609
Ama87I CYCGRG 1 cut(s) 351
AoxI GGCC 2 cut(s) 499, 1017
ApeKI GCWGC 6 cut(s) 11, 298, 301, 625, 900, 1213
ApoI RAATTY 3 cut(s) 291, 639, 720
Asp700I GAANNNNTTC 1 cut(s) 150
AspLEI GCGC 3 cut(s) 11, 870, 900
AspS9I GGNCC 1 cut(s) 1018
AsuHPI GGTGA 6 cut(s) 88, 407, 682, 739, 829, 944
AvaI CYCGRG 1 cut(s) 351
BanII GRGCYC 2 cut(s) 391, 744
BauI CACGAG 1 cut(s) 990
Bbv12I GWGCWC 2 cut(s) 744, 999
BbvI GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BccI CCATC 2 cut(s) 74, 1145
BceAI ACGGC 1 cut(s) 888
BciT130I CCWGG 4 cut(s) 329, 498, 694, 865
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 5 cut(s) 30, 534, 818, 922, 1050
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 3 cut(s) 438, 468, 951
BfmI CTRYAG 1 cut(s) 140
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 2 cut(s) 1030, 1098
BglII AGATCT 1 cut(s) 606
BisI GCNGC 6 cut(s) 12, 299, 302, 626, 901, 1214
BlsI GCNGC 6 cut(s) 13, 300, 303, 627, 902, 1215
Bme1390I CCNGG 4 cut(s) 329, 498, 694, 865
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 1018
BmrFI CCNGG 4 cut(s) 329, 498, 694, 865
BmsI GCATC 3 cut(s) 40, 885, 1102
BoxI GACNNNNGTC 1 cut(s) 440
BplI GAGNNNNNCTC 4 cut(s) 173, 205, 514, 546
BpuEI CTTGAG 2 cut(s) 571, 770
BsaI GGTCTC 3 cut(s) 30, 818, 922
BsaJI CCNNGG 5 cut(s) 403, 828, 928, 1021, 1152
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 1114, 1144
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse1I ACTGG 1 cut(s) 268
BseBI CCWGG 4 cut(s) 329, 498, 694, 865
BseDI CCNNGG 5 cut(s) 403, 828, 928, 1021, 1152
BseGI GGATG 4 cut(s) 558, 1162, 1173, 1179
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 41
BseNI ACTGG 1 cut(s) 268
BseRI GAGGAG 1 cut(s) 1223
BseXI GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BshFI GGCC 2 cut(s) 501, 1019
BsiHKAI GWGCWC 2 cut(s) 744, 999
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 2 cut(s) 17, 1079
BslFI GGGAC 4 cut(s) 150, 243, 448, 968
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 5 cut(s) 30, 534, 818, 922, 1050
BsmFI GGGAC 4 cut(s) 150, 243, 448, 968
BsnI GGCC 2 cut(s) 501, 1019
Bso31I GGTCTC 3 cut(s) 30, 818, 922
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 3 cut(s) 391, 744, 999
Bsp143I GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
Bsp19I CCATGG 1 cut(s) 928
BspACI CCGC 3 cut(s) 385, 471, 520
BspANI GGCC 2 cut(s) 501, 1019
BspCNI CTCAG 1 cut(s) 40
BspHI TCATGA 3 cut(s) 115, 702, 963
BspMAI CTGCAG 1 cut(s) 144
BspMI ACCTGC 2 cut(s) 1030, 1098
BspPI GGATC 2 cut(s) 82, 609
BspTNI GGTCTC 3 cut(s) 30, 818, 922
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 5 cut(s) 403, 828, 928, 1021, 1152
BssMI GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
BssSI CACGAG 1 cut(s) 990
BssT1I CCWWGG 3 cut(s) 828, 928, 1152
Bst2BI CACGAG 1 cut(s) 990
Bst2UI CCWGG 4 cut(s) 329, 498, 694, 865
Bst4CI ACNGT 2 cut(s) 122, 916
Bst6I CTCTTC 1 cut(s) 783
BstC8I GCNNGC 3 cut(s) 7, 387, 1218
BstDEI CTNAG 2 cut(s) 27, 155
BstDSI CCRYGG 2 cut(s) 928, 1021
BstF5I GGATG 4 cut(s) 558, 1162, 1173, 1179
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 3 cut(s) 11, 870, 900
BstKTI GATC 8 cut(s) 77, 454, 604, 609, 817, 910, 1012, 1234
BstMAI GTCTC 5 cut(s) 30, 534, 818, 922, 1050
BstMBI GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
BstMWI GCNNNNNNNGC 3 cut(s) 11, 805, 1226
BstNI CCWGG 4 cut(s) 329, 498, 694, 865
BstNSI RCATGY 1 cut(s) 517
BstPAI GACNNNNGTC 1 cut(s) 440
BstSCI CCNGG 4 cut(s) 327, 496, 692, 863
BstSFI CTRYAG 1 cut(s) 140
BstV1I GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BstX2I RGATCY 2 cut(s) 74, 606
BstYI RGATCY 2 cut(s) 74, 606
BsuRI GGCC 2 cut(s) 501, 1019
BtgI CCRYGG 2 cut(s) 928, 1021
BtsCI GGATG 4 cut(s) 558, 1162, 1173, 1179
BveI ACCTGC 2 cut(s) 1030, 1098
Cac8I GCNNGC 3 cut(s) 7, 387, 1218
CciI TCATGA 3 cut(s) 115, 702, 963
CfoI GCGC 3 cut(s) 11, 870, 900
Cfr13I GGNCC 1 cut(s) 1018
Csp6I GTAC 1 cut(s) 492
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 2 cut(s) 27, 155
DpnI GATC 8 cut(s) 76, 453, 603, 608, 816, 909, 1011, 1233
DpnII GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
EaeI YGGCCR 1 cut(s) 499
Eam1104I CTCTTC 1 cut(s) 783
EarI CTCTTC 1 cut(s) 783
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 3 cut(s) 828, 928, 1152
Eco24I GRGCYC 2 cut(s) 391, 744
Eco31I GGTCTC 3 cut(s) 30, 818, 922
Eco32I GATATC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 742
Eco88I CYCGRG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 742
EcoRII CCWGG 4 cut(s) 327, 496, 692, 863
EcoRV GATATC 1 cut(s) 688
EcoT14I CCWWGG 3 cut(s) 828, 928, 1152
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 2 cut(s) 391, 744
ErhI CCWWGG 3 cut(s) 828, 928, 1152
FaqI GGGAC 4 cut(s) 150, 243, 448, 968
FauI CCCGC 1 cut(s) 378
FauNDI CATATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 6 cut(s) 12, 299, 302, 626, 901, 1214
FokI GGATG 4 cut(s) 565, 1166, 1169, 1180
FriOI GRGCYC 2 cut(s) 391, 744
Fsp4HI GCNGC 6 cut(s) 12, 299, 302, 626, 901, 1214
FspBI CTAG 3 cut(s) 438, 468, 951
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 3 cut(s) 10, 869, 899
GluI GCNGC 6 cut(s) 12, 299, 302, 626, 901, 1214
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 501, 1019
HapII CCGG 2 cut(s) 17, 1079
HhaI GCGC 3 cut(s) 11, 870, 900
Hin6I GCGC 3 cut(s) 9, 868, 898
HinP1I GCGC 3 cut(s) 9, 868, 898
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 3 cut(s) 162, 474, 504
HpaII CCGG 2 cut(s) 17, 1079
HphI GGTGA 6 cut(s) 88, 407, 682, 739, 829, 944
Hpy166II GTNNAC 3 cut(s) 494, 781, 859
Hpy188I TCNGA 2 cut(s) 79, 606
Hpy188III TCNNGA 9 cut(s) 116, 258, 288, 539, 588, 703, 749, 964, 1183
Hpy8I GTNNAC 3 cut(s) 494, 781, 859
HpyAV CCTTC 6 cut(s) 14, 161, 229, 370, 1076, 1120
HpyCH4III ACNGT 2 cut(s) 122, 916
HpyCH4IV ACGT 2 cut(s) 457, 733
HpyCH4V TGCA 9 cut(s) 14, 142, 170, 194, 225, 298, 344, 546, 1093
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 805, 1226
HpyF3I CTNAG 2 cut(s) 27, 155
HpySE526I ACGT 2 cut(s) 457, 733
HspAI GCGC 3 cut(s) 9, 868, 898
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
LmnI GCTCC 3 cut(s) 633, 940, 1210
Lsp1109I GCAGC 5 cut(s) 310, 313, 612, 912, 1200
LweI GCATC 3 cut(s) 40, 885, 1102
MaeI CTAG 3 cut(s) 438, 468, 951
MaeII ACGT 2 cut(s) 457, 733
MaeIII GTNAC 3 cut(s) 395, 932, 1147
MalI GATC 8 cut(s) 76, 453, 603, 608, 816, 909, 1011, 1233
MboI GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
MboII GAAGA 5 cut(s) 85, 461, 800, 978, 1226
MflI RGATCY 2 cut(s) 74, 606
MhlI GDGCHC 3 cut(s) 391, 744, 999
MluCI AATT 6 cut(s) 195, 210, 291, 639, 720, 1203
MlyI GAGTC 2 cut(s) 483, 513
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 9 cut(s) 103, 109, 199, 413, 516, 584, 745, 1116, 1201
Mph1103I ATGCAT 1 cut(s) 172
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 2 cut(s) 759, 1056
MslI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 2 cut(s) 17, 1079
MspR9I CCNGG 4 cut(s) 329, 498, 694, 865
MvaI CCWGG 4 cut(s) 329, 498, 694, 865
MwoI GCNNNNNNNGC 3 cut(s) 11, 805, 1226
NcoI CCATGG 1 cut(s) 928
NdeI CATATG 1 cut(s) 172
NdeII GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
NmeAIII GCCGAG 1 cut(s) 527
NmuCI GTSAC 3 cut(s) 395, 932, 1147
NsbI TGCGCA 1 cut(s) 899
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 517
PagI TCATGA 3 cut(s) 115, 702, 963
PaqCI CACCTGC 1 cut(s) 1030
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 1102
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 6 cut(s) 13, 300, 303, 627, 902, 1215
PleI GAGTC 2 cut(s) 482, 512
PpsI GAGTC 2 cut(s) 482, 512
PscI ACATGT 1 cut(s) 513
PshAI GACNNNNGTC 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 4 cut(s) 327, 496, 692, 863
PspGI CCWGG 4 cut(s) 327, 496, 692, 863
PspPI GGNCC 1 cut(s) 1018
PstI CTGCAG 1 cut(s) 144
PsuI RGATCY 2 cut(s) 74, 606
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 2 cut(s) 759, 1056
SatI GCNGC 6 cut(s) 12, 299, 302, 626, 901, 1214
Sau3AI GATC 8 cut(s) 74, 451, 601, 606, 814, 907, 1009, 1231
Sau96I GGNCC 1 cut(s) 1018
SchI GAGTC 2 cut(s) 483, 513
ScrFI CCNGG 4 cut(s) 329, 498, 694, 865
SduI GDGCHC 3 cut(s) 391, 744, 999
SfaNI GCATC 3 cut(s) 40, 885, 1102
SfcI CTRYAG 1 cut(s) 140
SmiMI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
SmlI CTYRAG 2 cut(s) 586, 749
SmoI CTYRAG 2 cut(s) 586, 749
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 6 cut(s) 195, 210, 291, 639, 720, 1203
SsiI CCGC 3 cut(s) 385, 471, 520
SspMI CTAG 3 cut(s) 438, 468, 951
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 4 cut(s) 327, 496, 692, 863
StyI CCWWGG 3 cut(s) 828, 928, 1152
TaaI ACNGT 2 cut(s) 122, 916
TaiI ACGT 2 cut(s) 460, 736
TaqI TCGA 1 cut(s) 321
TaqII GACCGA 1 cut(s) 77
TasI AATT 6 cut(s) 195, 210, 291, 639, 720, 1203
TfiI GAWTC 1 cut(s) 162
Tru1I TTAA 2 cut(s) 759, 1056
Tru9I TTAA 2 cut(s) 759, 1056
TseFI GTSAC 3 cut(s) 395, 932, 1147
TseI GCWGC 6 cut(s) 11, 298, 301, 625, 900, 1213
Tsp45I GTSAC 3 cut(s) 395, 932, 1147
TspDTI ATGAA 7 cut(s) 104, 407, 582, 591, 691, 719, 1176
TspGWI ACGGA 2 cut(s) 434, 524
XapI RAATTY 3 cut(s) 291, 639, 720
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 2 cut(s) 485, 643
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 3 cut(s) 438, 468, 951
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.