FvH4_6g34120

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000799
Physical Location & Seq
Reverse (-)
26949279 .. 26952582
3304 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g34120.t2

Sequence Viewer

Length: 1242 bp
ATGGCAGGTCTGGCTCCCGAAGGTGCTCAATTTGATGCTAAACAGTATGATTCTAAGATGAATGAGATCCTCTCATCTGAAGGCCAAGAGTTCTTTACATCGTATGATGAGGTTCACGACAGTTTTGATGCCATGGGTTTACAAGAAAATCTTCTGCGTGGAATCTATGCTTACGGTTTTGAGAAGCCTTCTGCAATTCAGCAAAGGGGAATTGTTCCTTTTTGTAAAGGTTTGGATGTGATTCAACAAGCTCAGTCAGGAACAGGCAAAACAGCAACCTTCTGCTCGGGAATTTTGCAGCAACTTGACTATGGTTTGGTCCAGTGCCAGGCTTTGGTGTTGGCACCTACCAGGGAGTTGGCACAGCAGATTGAGAAGGTTATGCGGGCACTGGGTGACTATCTTGGTGTTAAGGTTCATGCTTGCGTAGGTGGGACTAGTGTTCGTGAGGATCAGCGAATTCTTCAAGCTGGTGTGCATGTGGTGGTTGGCACTCCTGGACGTGTGTTCGACATGTTGAGGAGGCAATCACTTCGTGCAGATTACATTAAGATGTTTGTTTTGGATGAAGCTGATGAAATGCTATCTAGGGGTTTCAAGGATCAGATCTATGACATTTTTCAGCTTCTGCCATCAAAAGTTCAAGTTGGTGTTTTCTCTGCTACTATGCCTCCTGAAGCCCTTGAGATCACCAGAAAGTTTATGAACAAACCTGTTAGGATTTTGGTTAAGCGTGATGAGCTCACCCTTGAGGGAATCAAGCAGTTTTATGTTAATGTTGACAAGGAAGAATGGAAGCTTGAGACACTTTGTGATCTCTACGAGACCCTTGCTATCACTCAGAGTGTCATTTTTGTTAATACAAGGCGCAAAGTGGATTGGCTTACAGATAAGATGCGAAGCCGTGACCACACAGTCTCTGCTACCCATGGTGACATGGACCAGAACCAGCGTGACATAATCATGCGTGAGTTTCGTTCTGGTTCCTCCCGTGTTCTCATCACAACTGATCTGTTGGCACGTGGTATTGATGTGCAGCAAGTGTCACTTGTAATTAATTATGATTTGCCAACTCAACCAGAAAACTACCTCCATCGTATTGGTCGAAGTGGTCGATTTGGAAGAAAAGGTGTTGCCATCAATTTTGTAACAAGGGATGATGAAAGAATGCTATTTGACATTCAGAGGTTTTATAATGTGGTGATTGAGGAGCTGCCTTCCAATGTGGCTGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

414

Amino Acids

46.88

Weight (kDa)

5.57

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 82 - 244 2.2e-43 DEAD/DEAH box helicase
ResIII PF04851 100 - 240 1.5e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 283 - 391 3.1e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1194
AasI GACNNNNNNGTC 1 cut(s) 914
AccB1I GGYRCC 1 cut(s) 343
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 1 cut(s) 385
AclWI GGATC 3 cut(s) 61, 459, 609
AcsI RAATTY 2 cut(s) 291, 459
AcuI CTGAAG 2 cut(s) 99, 696
AcvI CACGTG 1 cut(s) 1022
AdeI CACNNNGTG 3 cut(s) 395, 536, 812
AfiI CCNNNNNNNGG 2 cut(s) 328, 334
AflIII ACRYGT 2 cut(s) 502, 513
AgsI TTSAA 4 cut(s) 245, 467, 598, 644
AhlI ACTAGT 1 cut(s) 437
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 3 cut(s) 327, 350, 496
AluBI AGCT 7 cut(s) 251, 470, 572, 625, 742, 799, 1213
AluI AGCT 7 cut(s) 251, 470, 572, 625, 742, 799, 1213
Alw21I GWGCWC 2 cut(s) 28, 744
Alw26I GTCTC 3 cut(s) 797, 818, 922
AlwI GGATC 3 cut(s) 61, 459, 609
AlwNI CAGNNNCTG 2 cut(s) 628, 920
Ama87I CYCGRG 1 cut(s) 286
AoxI GGCC 1 cut(s) 82
ApeKI GCWGC 3 cut(s) 298, 1036, 1213
ApoI RAATTY 2 cut(s) 291, 459
AseI ATTAAT 1 cut(s) 1056
Asp700I GAANNNNTTC 1 cut(s) 150
AspLEI GCGC 1 cut(s) 870
AspS9I GGNCC 2 cut(s) 319, 940
AsuHPI GGTGA 5 cut(s) 407, 682, 736, 944, 1213
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 2 cut(s) 319, 940
BaeGI GKGCMC 1 cut(s) 391
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 744
BbrPI CACGTG 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 28, 744
BbvI GCAGC 3 cut(s) 310, 1048, 1200
BccI CCATC 3 cut(s) 640, 1101, 1145
BceAI ACGGC 1 cut(s) 888
BcgI CGANNNNNNTGC 4 cut(s) 515, 549, 812, 846
BciT130I CCWGG 3 cut(s) 329, 352, 498
BcoDI GTCTC 3 cut(s) 797, 818, 922
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 2 cut(s) 438, 588
BglII AGATCT 1 cut(s) 606
BisI GCNGC 3 cut(s) 299, 1037, 1214
BlsI GCNGC 3 cut(s) 300, 1038, 1215
Bme1390I CCNGG 3 cut(s) 329, 352, 498
Bme18I GGWCC 2 cut(s) 319, 940
BmeT110I CYCGRG 1 cut(s) 286
BmgBI CACGTC 1 cut(s) 503
BmgT120I GGNCC 2 cut(s) 319, 940
BmiI GGNNCC 3 cut(s) 15, 345, 985
BmrFI CCNGG 3 cut(s) 329, 352, 498
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 3 cut(s) 25, 118, 885
BmuI ACTGGG 1 cut(s) 401
BplI GAGNNNNNCTC 2 cut(s) 56, 88
BpuEI CTTGAG 3 cut(s) 704, 770, 821
BsaAI YACGTR 1 cut(s) 1022
BsaI GGTCTC 1 cut(s) 818
BsaJI CCNNGG 3 cut(s) 132, 351, 928
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 334
Bse1I ACTGG 2 cut(s) 322, 396
BseBI CCWGG 3 cut(s) 329, 352, 498
BseDI CCNNGG 3 cut(s) 132, 351, 928
BseGI GGATG 3 cut(s) 241, 571, 1162
BseLI CCNNNNNNNGG 2 cut(s) 328, 334
BseMII CTCAG 2 cut(s) 266, 854
BseNI ACTGG 2 cut(s) 322, 396
BseRI GAGGAG 2 cut(s) 535, 1223
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 3 cut(s) 310, 1048, 1200
BsgI GTGCAG 2 cut(s) 558, 1055
BshFI GGCC 1 cut(s) 84
BshNI GGYRCC 1 cut(s) 343
BsiHKAI GWGCWC 2 cut(s) 28, 744
BsiHKCI CYCGRG 1 cut(s) 286
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 2 cut(s) 328, 334
BsmAI GTCTC 3 cut(s) 797, 818, 922
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 1 cut(s) 1173
BsnI GGCC 1 cut(s) 84
Bso31I GGTCTC 1 cut(s) 818
BsoBI CYCGRG 1 cut(s) 286
Bsp1286I GDGCHC 3 cut(s) 28, 391, 744
Bsp143I GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
Bsp19I CCATGG 2 cut(s) 132, 928
BspACI CCGC 1 cut(s) 385
BspANI GGCC 1 cut(s) 84
BspCNI CTCAG 2 cut(s) 265, 853
BspLI GGNNCC 3 cut(s) 15, 345, 985
BspPI GGATC 3 cut(s) 61, 459, 609
BspT107I GGYRCC 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 818
BsrI ACTGG 2 cut(s) 322, 396
BssECI CCNNGG 3 cut(s) 132, 351, 928
BssMI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
BssT1I CCWWGG 2 cut(s) 132, 928
Bst2UI CCWGG 3 cut(s) 329, 352, 498
Bst4CI ACNGT 4 cut(s) 45, 122, 176, 916
BstBAI YACGTR 1 cut(s) 1022
BstC8I GCNNGC 2 cut(s) 387, 424
BstDEI CTNAG 3 cut(s) 54, 252, 840
BstDSI CCRYGG 2 cut(s) 132, 928
BstF5I GGATG 3 cut(s) 241, 571, 1162
BstHHI GCGC 1 cut(s) 870
BstKTI GATC 8 cut(s) 69, 454, 604, 609, 690, 817, 1012, 1234
BstMAI GTCTC 3 cut(s) 797, 818, 922
BstMBI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 11, 739
BstNI CCWGG 3 cut(s) 329, 352, 498
BstNSI RCATGY 2 cut(s) 482, 517
BstSCI CCNGG 3 cut(s) 327, 350, 496
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 3 cut(s) 310, 1048, 1200
BstX2I RGATCY 2 cut(s) 66, 606
BstXI CCANNNNNNTGG 2 cut(s) 358, 1100
BstYI RGATCY 2 cut(s) 66, 606
BsuRI GGCC 1 cut(s) 84
BtgI CCRYGG 2 cut(s) 132, 928
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 3 cut(s) 241, 571, 1162
BtsIMutI CAGTG 2 cut(s) 329, 389
Cac8I GCNNGC 2 cut(s) 387, 424
CaiI CAGNNNCTG 2 cut(s) 628, 920
CfoI GCGC 1 cut(s) 870
Cfr13I GGNCC 2 cut(s) 319, 940
CviAII CATG 7 cut(s) 133, 419, 479, 514, 929, 937, 964
DdeI CTNAG 3 cut(s) 54, 252, 840
DpnI GATC 8 cut(s) 68, 453, 603, 608, 689, 816, 1011, 1233
DpnII GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
DraIII CACNNNGTG 3 cut(s) 395, 536, 812
DrdI GACNNNNNNGTC 1 cut(s) 914
DseDI GACNNNNNNGTC 1 cut(s) 914
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 2 cut(s) 132, 928
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 818
Eco47I GGWCC 2 cut(s) 319, 940
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 2 cut(s) 99, 696
Eco72I CACGTG 1 cut(s) 1022
Eco88I CYCGRG 1 cut(s) 286
EcoICRI GAGCTC 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 459
EcoRII CCWGG 3 cut(s) 327, 350, 496
EcoT14I CCWWGG 2 cut(s) 132, 928
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 2 cut(s) 132, 928
FaeI CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 1032, 1064
FaqI GGGAC 1 cut(s) 448
FatI CATG 7 cut(s) 132, 418, 478, 513, 928, 936, 963
FauI CCCGC 1 cut(s) 378
Fnu4HI GCNGC 3 cut(s) 299, 1037, 1214
FokI GGATG 3 cut(s) 248, 578, 1169
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 3 cut(s) 299, 1037, 1214
FspBI CTAG 2 cut(s) 438, 588
GlaI GCGC 1 cut(s) 869
GluI GCNGC 3 cut(s) 299, 1037, 1214
HaeIII GGCC 1 cut(s) 84
HhaI GCGC 1 cut(s) 870
Hin1II CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
Hin6I GCGC 1 cut(s) 868
HinP1I GCGC 1 cut(s) 868
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 4 cut(s) 50, 162, 241, 756
HphI GGTGA 5 cut(s) 407, 682, 736, 944, 1213
Hpy166II GTNNAC 3 cut(s) 115, 140, 781
Hpy188I TCNGA 4 cut(s) 79, 606, 843, 1185
Hpy188III TCNNGA 6 cut(s) 17, 116, 258, 288, 446, 674
Hpy8I GTNNAC 3 cut(s) 115, 140, 781
HpyAV CCTTC 6 cut(s) 14, 74, 198, 289, 370, 1227
HpyCH4III ACNGT 4 cut(s) 45, 122, 176, 916
HpyCH4IV ACGT 2 cut(s) 502, 1021
HpyCH4V TGCA 5 cut(s) 194, 298, 478, 539, 1036
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 739
HpyF3I CTNAG 3 cut(s) 54, 252, 840
HpySE526I ACGT 2 cut(s) 502, 1021
Hsp92II CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
HspAI GCGC 1 cut(s) 868
Kzo9I GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
LmnI GCTCC 2 cut(s) 19, 1210
Lsp1109I GCAGC 3 cut(s) 310, 1048, 1200
LweI GCATC 3 cut(s) 25, 118, 885
MaeI CTAG 2 cut(s) 438, 588
MaeII ACGT 2 cut(s) 502, 1021
MaeIII GTNAC 6 cut(s) 395, 905, 932, 953, 1044, 1147
MalI GATC 8 cut(s) 68, 453, 603, 608, 689, 816, 1011, 1233
MboI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
MboII GAAGA 5 cut(s) 143, 455, 800, 1134, 1226
MflI RGATCY 2 cut(s) 66, 606
MhlI GDGCHC 3 cut(s) 28, 391, 744
MluCI AATT 8 cut(s) 29, 195, 210, 291, 459, 1053, 1057, 1141
MroXI GAANNNNTTC 1 cut(s) 150
MseI TTAA 6 cut(s) 411, 549, 729, 774, 858, 1056
MslI CAYNNNNRTG 2 cut(s) 551, 962
MspR9I CCNGG 3 cut(s) 329, 352, 498
Mva1269I GAATGC 1 cut(s) 1173
MvaI CCWGG 3 cut(s) 329, 352, 498
MwoI GCNNNNNNNGC 2 cut(s) 11, 739
NcoI CCATGG 2 cut(s) 132, 928
NdeII GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
NlaIII CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
NlaIV GGNNCC 3 cut(s) 15, 345, 985
NmuCI GTSAC 5 cut(s) 395, 905, 932, 953, 1044
NspI RCATGY 2 cut(s) 482, 517
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 2 cut(s) 1102, 1111
PctI GAATGC 1 cut(s) 1173
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 4 cut(s) 50, 162, 241, 756
PflMI CCANNNNNTGG 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 3 cut(s) 300, 1038, 1215
PmaCI CACGTG 1 cut(s) 1022
PmlI CACGTG 1 cut(s) 1022
Ppu21I YACGTR 1 cut(s) 1022
PscI ACATGT 1 cut(s) 513
PshBI ATTAAT 1 cut(s) 1056
PsiI TTATAA 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 3 cut(s) 327, 350, 496
PspCI CACGTG 1 cut(s) 1022
PspGI CCWGG 3 cut(s) 327, 350, 496
PspN4I GGNNCC 3 cut(s) 15, 345, 985
PspPI GGNCC 2 cut(s) 319, 940
PstNI CAGNNNCTG 2 cut(s) 628, 920
PsuI RGATCY 2 cut(s) 66, 606
RseI CAYNNNNRTG 2 cut(s) 551, 962
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 6 cut(s) 411, 549, 729, 774, 858, 1056
SatI GCNGC 3 cut(s) 299, 1037, 1214
Sau3AI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 1009, 1231
Sau96I GGNCC 2 cut(s) 319, 940
ScrFI CCNGG 3 cut(s) 329, 352, 498
SduI GDGCHC 3 cut(s) 28, 391, 744
SfaNI GCATC 3 cut(s) 25, 118, 885
SinI GGWCC 2 cut(s) 319, 940
SmiMI CAYNNNNRTG 2 cut(s) 551, 962
SmlI CTYRAG 3 cut(s) 683, 749, 800
SmoI CTYRAG 3 cut(s) 683, 749, 800
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 8 cut(s) 29, 195, 210, 291, 459, 1053, 1057, 1141
SsiI CCGC 1 cut(s) 385
SspMI CTAG 2 cut(s) 438, 588
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 327, 350, 496
StyI CCWWGG 2 cut(s) 132, 928
TaaI ACNGT 4 cut(s) 45, 122, 176, 916
TaiI ACGT 2 cut(s) 505, 1024
TaqI TCGA 3 cut(s) 510, 1105, 1114
TasI AATT 8 cut(s) 29, 195, 210, 291, 459, 1053, 1057, 1141
TfiI GAWTC 4 cut(s) 50, 162, 241, 756
Tru1I TTAA 6 cut(s) 411, 549, 729, 774, 858, 1056
Tru9I TTAA 6 cut(s) 411, 549, 729, 774, 858, 1056
TscAI CASTG 2 cut(s) 329, 396
TseFI GTSAC 5 cut(s) 395, 905, 932, 953, 1044
TseI GCWGC 3 cut(s) 298, 1036, 1213
Tsp45I GTSAC 5 cut(s) 395, 905, 932, 953, 1044
TspDTI ATGAA 6 cut(s) 74, 407, 582, 591, 719, 1176
TspRI CASTG 2 cut(s) 329, 396
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 319, 940
VspI ATTAAT 1 cut(s) 1056
XapI RAATTY 2 cut(s) 291, 459
XceI RCATGY 2 cut(s) 482, 517
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 2 cut(s) 438, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.