Prupe.1G409000_v2.0.a1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000799
Physical Location & Seq
Forward (+)
35801076 .. 35804374
3299 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G409000.1

Sequence Viewer

Length: 1242 bp
ATGGCTGCTGTGGCACCGGAAGGATCCCAATTTGATGCTCGTCAGTATGATGCTAAAATGAGTGAGTTACTTGGAGCTGATGGGCAAGAATTCTTCACATCATATGATGAGGTTCATGAGAGCTTTGATGCTATGGGATTGCAGGAGAACCTTCTTAGGGGCATTTATGCATATGGTTTTGAGAAGCCCTCTGCAATTCAGCAAAGGGGAATTGTTCCTTTCTGCAAGGGACTTGATGTGATTCAACAGGCACAGTCTGGAACTGGAAAAACTGCGACTTTCTGCTCTGGAATTCTGCAGCAGCTTGATTATGCCTTACCTGATTGCCAGGCATTGGTTCTTGCACCCACACGAGAACTTGCTCAACAGATTGAGAAGGTTATGCGAGCACTGGGGGACTATCTTGGTGTCAAGGTTCATGCTTGTGTTGGTGGGACCAGTGTTCGTGAAGATCAACGCATTCTCTCCAGTGGGGTCCATGTCGTTGTTGGTACACCTGGTCGTGTATTTGACATGTTACGGAGACAGTCTCTTCGTCCAGATTACATTAAGATGTTTGTCTTAGATGAAGCAGATGAAATGCTTTCACGAGGTTTCAAGGATCAGATCTATGACATTTTCCAGCTGCTACCATCGAAAATTCAGGTGGGGGTTTTCTCTGCCACAATGCCACCGGAGGCCCTTGACATCACTAGGAAGTTCATGAATAAACCTGTGAGGATTTTGGTGAAACGTGATGAGCTCACCCTTGAGGGTATCAAGCAATTCCATGTCAATGTTGATAAAGAGGAATGGAAACTTGAGACACTTTGTGATCTTTATGAGACCTTGGCAATTACTCAGAGTGTCATCTTTGTGAACACCAGGCGCAAAGTTGATTGGCTCACTGACAAGATGCGCAGCCGCGATCACACAGTCTCTGCCACCCATGGAGACATGGACCAAAACACAAGAGACATTATCATGAGAGAGTTTCGGTCTGGTTCTTCTCGTGTGCTCATAACAACTGATCTCTTGGCCCGTGGTATTGATGTCCAGCAGGTCTCTTTAGTGATTAATTTTGATCTTCCAACACAACCGGAAAACTACCTCCACCGCATTGGTCGTAGTGGTCGTTTCGGGAGGAAGGGTGTTGCCATCAATTTTGTGACCAAGGATGACGACAGGATGTTGTTTGACATCCAGAAGTTTTATAATGTTGTGATTGAGGAGCTGCCGGCAAATGTTGCTGATCTGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

414

Amino Acids

46.67

Weight (kDa)

5.53

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1194
Acc16I TGCGCA 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 1030
AccB1I GGYRCC 1 cut(s) 13
AccB7I CCANNNNNTGG 1 cut(s) 334
AccII CGCG 1 cut(s) 906
AciI CCGC 2 cut(s) 904, 1096
AclWI GGATC 3 cut(s) 18, 31, 609
AcsI RAATTY 3 cut(s) 89, 291, 639
AdeI CACNNNGTG 1 cut(s) 812
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 2 cut(s) 157, 334
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 2 cut(s) 245, 598
AjnI CCWGG 3 cut(s) 327, 496, 863
AluBI AGCT 6 cut(s) 77, 123, 304, 625, 742, 1213
AluI AGCT 6 cut(s) 77, 123, 304, 625, 742, 1213
Alw21I GWGCWC 3 cut(s) 391, 744, 999
Alw26I GTCTC 8 cut(s) 517, 534, 797, 818, 922, 927, 948, 1048
AlwI GGATC 3 cut(s) 18, 31, 609
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 2 cut(s) 678, 1017
ApeKI GCWGC 6 cut(s) 5, 298, 301, 625, 900, 1213
ApoI RAATTY 3 cut(s) 89, 291, 639
ArsI GACNNNNNNTTYG 2 cut(s) 516, 548
AseI ATTAAT 1 cut(s) 1056
AspLEI GCGC 2 cut(s) 870, 900
AspS9I GGNCC 5 cut(s) 435, 475, 679, 940, 1018
AsuHPI GGTGA 2 cut(s) 736, 739
AvaII GGWCC 3 cut(s) 435, 475, 940
BamHI GGATCC 1 cut(s) 23
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 744
BauI CACGAG 3 cut(s) 351, 588, 990
Bbv12I GWGCWC 3 cut(s) 391, 744, 999
BbvI GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BccI CCATC 3 cut(s) 74, 640, 1145
BciT130I CCWGG 3 cut(s) 329, 498, 865
BcoDI GTCTC 8 cut(s) 517, 534, 797, 818, 922, 927, 948, 1048
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 1 cut(s) 296
BfuAI ACCTGC 1 cut(s) 1030
BglII AGATCT 1 cut(s) 606
BisI GCNGC 7 cut(s) 6, 299, 302, 626, 901, 904, 1214
BlsI GCNGC 7 cut(s) 7, 300, 303, 627, 902, 905, 1215
Bme1390I CCNGG 3 cut(s) 329, 498, 865
Bme18I GGWCC 3 cut(s) 435, 475, 940
BmgT120I GGNCC 5 cut(s) 435, 475, 679, 940, 1018
BmiI GGNNCC 4 cut(s) 15, 25, 436, 476
BmrFI CCNGG 3 cut(s) 329, 498, 865
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 4 cut(s) 25, 40, 118, 885
BmuI ACTGGG 1 cut(s) 401
BplI GAGNNNNNCTC 4 cut(s) 173, 205, 514, 546
BpmI CTGGAG 1 cut(s) 451
BpuEI CTTGAG 2 cut(s) 770, 821
BsaI GGTCTC 2 cut(s) 818, 1048
BsaJI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BsaWI WCCGGW 3 cut(s) 16, 673, 1078
BsaXI ACNNNNNCTCC 2 cut(s) 1114, 1144
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 334
Bse118I RCCGGY 1 cut(s) 1216
Bse1I ACTGG 4 cut(s) 268, 396, 438, 468
BseBI CCWGG 3 cut(s) 329, 498, 865
BseDI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BseGI GGATG 3 cut(s) 1162, 1173, 1179
BseLI CCNNNNNNNGG 2 cut(s) 157, 334
BseMII CTCAG 1 cut(s) 854
BseNI ACTGG 4 cut(s) 268, 396, 438, 468
BseRI GAGGAG 1 cut(s) 1223
BseXI GCAGC 5 cut(s) 310, 313, 612, 912, 1200
Bsh1236I CGCG 1 cut(s) 906
BshFI GGCC 2 cut(s) 680, 1019
BshNI GGYRCC 1 cut(s) 13
BsiHKAI GWGCWC 3 cut(s) 391, 744, 999
BsiSI CCGG 4 cut(s) 17, 674, 1079, 1217
BslFI GGGAC 3 cut(s) 243, 410, 448
BslI CCNNNNNNNGG 2 cut(s) 157, 334
BsmAI GTCTC 8 cut(s) 517, 534, 797, 818, 922, 927, 948, 1048
BsmFI GGGAC 3 cut(s) 243, 410, 448
BsmI GAATGC 1 cut(s) 459
BsnI GGCC 2 cut(s) 680, 1019
Bso31I GGTCTC 2 cut(s) 818, 1048
Bsp1286I GDGCHC 3 cut(s) 391, 744, 999
Bsp143I GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
Bsp19I CCATGG 1 cut(s) 928
BspACI CCGC 2 cut(s) 904, 1096
BspANI GGCC 2 cut(s) 680, 1019
BspCNI CTCAG 1 cut(s) 853
BspFNI CGCG 1 cut(s) 906
BspHI TCATGA 3 cut(s) 115, 702, 963
BspLI GGNNCC 4 cut(s) 15, 25, 436, 476
BspMAI CTGCAG 1 cut(s) 300
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 3 cut(s) 18, 31, 609
BspT107I GGYRCC 1 cut(s) 13
BspTNI GGTCTC 2 cut(s) 818, 1048
BsrFI RCCGGY 1 cut(s) 1216
BsrI ACTGG 4 cut(s) 268, 396, 438, 468
BssAI RCCGGY 1 cut(s) 1216
BssECI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BssMI GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
BssSI CACGAG 3 cut(s) 351, 588, 990
BssT1I CCWWGG 3 cut(s) 828, 928, 1152
Bst2BI CACGAG 3 cut(s) 351, 588, 990
Bst2UI CCWGG 3 cut(s) 329, 498, 865
Bst4CI ACNGT 3 cut(s) 255, 528, 916
Bst6I CTCTTC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 1226
BstC8I GCNNGC 2 cut(s) 387, 1218
BstDEI CTNAG 3 cut(s) 155, 562, 840
BstDSI CCRYGG 2 cut(s) 928, 1021
BstF5I GGATG 3 cut(s) 1162, 1173, 1179
BstFNI CGCG 1 cut(s) 906
BstHHI GCGC 2 cut(s) 870, 900
BstKTI GATC 9 cut(s) 26, 454, 604, 609, 817, 910, 1012, 1066, 1234
BstMAI GTCTC 8 cut(s) 517, 534, 797, 818, 922, 927, 948, 1048
BstMBI GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 11, 1226
BstNI CCWGG 3 cut(s) 329, 498, 865
BstNSI RCATGY 1 cut(s) 517
BstSCI CCNGG 3 cut(s) 327, 496, 863
BstSFI CTRYAG 1 cut(s) 296
BstUI CGCG 1 cut(s) 906
BstV1I GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BstX2I RGATCY 2 cut(s) 23, 606
BstXI CCANNNNNNTGG 1 cut(s) 1100
BstYI RGATCY 2 cut(s) 23, 606
BsuRI GGCC 2 cut(s) 680, 1019
BtgI CCRYGG 2 cut(s) 928, 1021
BtsCI GGATG 3 cut(s) 1162, 1173, 1179
BtsIMutI CAGTG 4 cut(s) 389, 445, 475, 885
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 2 cut(s) 387, 1218
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 3 cut(s) 115, 702, 963
CfoI GCGC 2 cut(s) 870, 900
Cfr10I RCCGGY 1 cut(s) 1216
Cfr13I GGNCC 5 cut(s) 435, 475, 679, 940, 1018
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 1 cut(s) 492
CviAII CATG 9 cut(s) 116, 419, 479, 514, 703, 770, 929, 937, 964
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 3 cut(s) 155, 562, 840
DpnI GATC 9 cut(s) 25, 453, 603, 608, 816, 909, 1011, 1065, 1233
DpnII GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
DraIII CACNNNGTG 1 cut(s) 812
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 3 cut(s) 828, 928, 1152
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 2 cut(s) 818, 1048
Eco47I GGWCC 3 cut(s) 435, 475, 940
Eco53kI GAGCTC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 679
EcoRI GAATTC 2 cut(s) 89, 291
EcoRII CCWGG 3 cut(s) 327, 496, 863
EcoT14I CCWWGG 3 cut(s) 828, 928, 1152
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 3 cut(s) 828, 928, 1152
FaeI CATG 9 cut(s) 119, 422, 482, 517, 706, 773, 932, 940, 967
FaqI GGGAC 3 cut(s) 243, 410, 448
FatI CATG 9 cut(s) 115, 418, 478, 513, 702, 769, 928, 936, 963
FauNDI CATATG 2 cut(s) 103, 172
Fnu4HI GCNGC 7 cut(s) 6, 299, 302, 626, 901, 904, 1214
FokI GGATG 3 cut(s) 1166, 1169, 1180
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 7 cut(s) 6, 299, 302, 626, 901, 904, 1214
FspBI CTAG 1 cut(s) 693
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 2 cut(s) 869, 899
GluI GCNGC 7 cut(s) 6, 299, 302, 626, 901, 904, 1214
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 2 cut(s) 680, 1019
HapII CCGG 4 cut(s) 17, 674, 1079, 1217
HhaI GCGC 2 cut(s) 870, 900
Hin1II CATG 9 cut(s) 119, 422, 482, 517, 706, 773, 932, 940, 967
Hin6I GCGC 2 cut(s) 868, 898
HinP1I GCGC 2 cut(s) 868, 898
HinfI GANTC 1 cut(s) 241
HpaII CCGG 4 cut(s) 17, 674, 1079, 1217
HphI GGTGA 2 cut(s) 736, 739
Hpy166II GTNNAC 2 cut(s) 494, 859
Hpy188I TCNGA 2 cut(s) 606, 843
Hpy8I GTNNAC 2 cut(s) 494, 859
HpyAV CCTTC 4 cut(s) 14, 161, 370, 1120
HpyCH4III ACNGT 3 cut(s) 255, 528, 916
HpyCH4IV ACGT 1 cut(s) 733
HpyCH4V TGCA 6 cut(s) 142, 170, 194, 225, 298, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 1226
HpyF3I CTNAG 3 cut(s) 155, 562, 840
HpySE526I ACGT 1 cut(s) 733
Hsp92II CATG 9 cut(s) 119, 422, 482, 517, 706, 773, 932, 940, 967
HspAI GCGC 2 cut(s) 868, 898
KroI GCCGGC 1 cut(s) 1216
KroNI GCCGGC 1 cut(s) 1218
Kzo9I GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
LmnI GCTCC 2 cut(s) 74, 1210
Lsp1109I GCAGC 5 cut(s) 310, 313, 612, 912, 1200
LweI GCATC 4 cut(s) 25, 40, 118, 885
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 1 cut(s) 693
MaeII ACGT 1 cut(s) 733
MaeIII GTNAC 3 cut(s) 66, 516, 1147
MalI GATC 9 cut(s) 25, 453, 603, 608, 816, 909, 1011, 1065, 1233
MboI GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
MboII GAAGA 5 cut(s) 85, 461, 524, 978, 1058
MflI RGATCY 2 cut(s) 23, 606
MhlI GDGCHC 3 cut(s) 391, 744, 999
MmeI TCCRAC 1 cut(s) 1094
Mph1103I ATGCAT 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 1216
MseI TTAA 3 cut(s) 549, 1056, 1240
MslI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 4 cut(s) 17, 674, 1079, 1217
MspR9I CCNGG 3 cut(s) 329, 498, 865
Mva1269I GAATGC 1 cut(s) 459
MvaI CCWGG 3 cut(s) 329, 498, 865
MvnI CGCG 1 cut(s) 906
MwoI GCNNNNNNNGC 2 cut(s) 11, 1226
NaeI GCCGGC 1 cut(s) 1218
NcoI CCATGG 1 cut(s) 928
NdeI CATATG 2 cut(s) 103, 172
NdeII GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
NgoMIV GCCGGC 1 cut(s) 1216
NlaIII CATG 9 cut(s) 119, 422, 482, 517, 706, 773, 932, 940, 967
NlaIV GGNNCC 4 cut(s) 15, 25, 436, 476
NmuCI GTSAC 1 cut(s) 1147
NsbI TGCGCA 1 cut(s) 899
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 517
PagI TCATGA 3 cut(s) 115, 702, 963
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 1111
PctI GAATGC 1 cut(s) 459
PdiI GCCGGC 1 cut(s) 1218
PfeI GAWTC 1 cut(s) 241
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 7 cut(s) 7, 300, 303, 627, 902, 905, 1215
PscI ACATGT 1 cut(s) 513
PshBI ATTAAT 1 cut(s) 1056
PsiI TTATAA 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 3 cut(s) 327, 496, 863
PspGI CCWGG 3 cut(s) 327, 496, 863
PspN4I GGNNCC 4 cut(s) 15, 25, 436, 476
PspPI GGNCC 5 cut(s) 435, 475, 679, 940, 1018
PstI CTGCAG 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 2 cut(s) 23, 606
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 3 cut(s) 549, 1056, 1240
SatI GCNGC 7 cut(s) 6, 299, 302, 626, 901, 904, 1214
Sau3AI GATC 9 cut(s) 23, 451, 601, 606, 814, 907, 1009, 1063, 1231
Sau96I GGNCC 5 cut(s) 435, 475, 679, 940, 1018
ScrFI CCNGG 3 cut(s) 329, 498, 865
SduI GDGCHC 3 cut(s) 391, 744, 999
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 4 cut(s) 25, 40, 118, 885
SfcI CTRYAG 1 cut(s) 296
SinI GGWCC 3 cut(s) 435, 475, 940
SmiMI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
SmlI CTYRAG 2 cut(s) 749, 800
SmoI CTYRAG 2 cut(s) 749, 800
SsiI CCGC 2 cut(s) 904, 1096
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 327, 496, 863
StyI CCWWGG 3 cut(s) 828, 928, 1152
TaaI ACNGT 3 cut(s) 255, 528, 916
TaiI ACGT 1 cut(s) 736
TaqI TCGA 1 cut(s) 635
TaqII GACCGA 1 cut(s) 966
TauI GCSGC 1 cut(s) 906
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 3 cut(s) 549, 1056, 1240
Tru9I TTAA 3 cut(s) 549, 1056, 1240
TscAI CASTG 4 cut(s) 396, 445, 475, 892
TseFI GTSAC 1 cut(s) 1147
TseI GCWGC 6 cut(s) 5, 298, 301, 625, 900, 1213
Tsp45I GTSAC 1 cut(s) 1147
TspDTI ATGAA 6 cut(s) 104, 407, 582, 591, 691, 719
TspGWI ACGGA 1 cut(s) 535
TspRI CASTG 4 cut(s) 396, 445, 475, 892
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 3 cut(s) 435, 475, 940
VspI ATTAAT 1 cut(s) 1056
XapI RAATTY 3 cut(s) 89, 291, 639
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XspI CTAG 1 cut(s) 693
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.