pycom02g06560

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000799
Physical Location & Seq
Reverse (-)
4562370 .. 4564406
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06560.4

Sequence Viewer

Length: 1185 bp
ATGAGCGAAATACTTGCAACTGATGGGCAGGAATTCTTTACATCGTATGATGAAGTTTATGATAGTTTTGATGCTATGGGTCTTCAAGAGAATCTTCTGAGGGGAATCTATGCTTACGGTTTTGAAAAACCCTCGGCTATTCAGCAAAGAGGAATTGTTCCCTTCTGCAAGGGTCTTGATGTGATTCAACAGGCTCAGTCTGGAACTGGAAAGACAGCAACTTTCTGTTCTGGTATTCTTCAACAGCTTGATTATAGTTTGACAGAATGCCAAGCCTTGGTTCTTGCACCCACTCGAGAGCTTGCTCAACAGATTGAGAAGGTTATGAGGGCTCTTGGAGACTATCTAGGTGTGAGGGTGCATGCTTGTGTTGGTGGAACTAGTGTCCGTGAGGACCAACGCATTCTGTCAAATGGAGTTCATGTTGTTGTTGGAACTCCAGGTCGTGTTTTTGATATGCTTAGAAGGCAGTCACTTCGTCCTGGTAGCATCAAGATGTTTGTTTTGGATGAGGCTGATGAAATGCTCTCCCGTGGTTTCAAGGATCAGATCTACGACATATTCCAGCTTCTTCCATCAAAGATTCAGGTTGGTGTGTTCTCAGCCACAATGCCGCCTGAGGCCCTCGAGATCACAAGGAAGTTCATGAACAAACCCGTAAGGATTCTTGTGAAGCGTGATGAACTCACCCTGGAAGGTATAAAGCAATTCTATGTCAATGTCGACAAGGAGGAATGGAAGCTTGAGACTCTTTGCGATCTGTATGAGACCTTGGCTATTACCCAGAGTGTCATTTTTGTTAATACTCGACGCAAGGTTGACTGGTTGACTGACAAGATGAGGAGCCGAGACCATACAGTTTCTGCCACCCATGGAGACATGGACCAGAACACCAGAGACATCATCATGCGGGAGTTTCGTTCTGGATCATCTCGTGTCTTGATCACCACTGATCTTTTGGCTCGTGGTATTGATGTTCAGCAAGTCTCCCTAGTTATTAACTATGATCTGCCCACCCAGCCCGAGAACTACCTTCACAGAATTGGTCGTAGTGGACGTTTTGGAAGGAAGGGTGTTGCCATCAACTTCGTAACGAAGGATGATGAGAGGATGCTGTTTGACATCCAGAAGTTCTATAACGTGGTTGTGGAGGAGCTGCCTTCAAATGTTGCGGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

395

Amino Acids

44.72

Weight (kDa)

5.58

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 277
AccI GTMKAC 1 cut(s) 723
AciI CCGC 3 cut(s) 614, 910, 1172
AclWI GGATC 3 cut(s) 552, 934, 1182
AcsI RAATTY 1 cut(s) 32
AfiI CCNNNNNNNGG 1 cut(s) 277
AgsI TTSAA 6 cut(s) 86, 125, 188, 242, 541, 1164
AhlI ACTAGT 1 cut(s) 380
AjnI CCWGG 3 cut(s) 439, 481, 690
AjuI GAANNNNNNNTTGG 2 cut(s) 264, 296
AluBI AGCT 5 cut(s) 247, 301, 568, 742, 1156
AluI AGCT 5 cut(s) 247, 301, 568, 742, 1156
Alw26I GTCTC 7 cut(s) 333, 740, 761, 843, 870, 891, 991
AlwI GGATC 3 cut(s) 552, 934, 1182
AlwNI CAGNNNCTG 1 cut(s) 863
Ama87I CYCGRG 3 cut(s) 294, 626, 1022
AoxI GGCC 1 cut(s) 621
ApeKI GCWGC 1 cut(s) 1156
ApoI RAATTY 1 cut(s) 32
AspS9I GGNCC 3 cut(s) 394, 622, 883
AsuHPI GGTGA 2 cut(s) 679, 937
AvaI CYCGRG 3 cut(s) 294, 626, 1022
AvaII GGWCC 2 cut(s) 394, 883
AxyI CCTNAGG 1 cut(s) 618
BanII GRGCYC 1 cut(s) 334
BauI CACGAG 2 cut(s) 933, 963
BbsI GAAGAC 1 cut(s) 74
BbvI GCAGC 1 cut(s) 1143
BccI CCATC 3 cut(s) 17, 583, 1088
BcgI CGANNNNNNTGC 3 cut(s) 30, 458, 492
BciT130I CCWGG 3 cut(s) 441, 483, 692
BclI TGATCA 1 cut(s) 942
BcoDI GTCTC 7 cut(s) 333, 740, 761, 843, 870, 891, 991
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 3 cut(s) 347, 381, 992
BglII AGATCT 1 cut(s) 549
BisI GCNGC 2 cut(s) 614, 1157
BlsI GCNGC 2 cut(s) 615, 1158
Bme1390I CCNGG 3 cut(s) 441, 483, 692
Bme18I GGWCC 2 cut(s) 394, 883
BmeT110I CYCGRG 3 cut(s) 294, 626, 1022
BmgT120I GGNCC 3 cut(s) 394, 622, 883
BmiI GGNNCC 1 cut(s) 845
BmrFI CCNGG 3 cut(s) 441, 483, 692
BmsI GCATC 3 cut(s) 61, 498, 1101
BpiI GAAGAC 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 764
BsaI GGTCTC 2 cut(s) 761, 843
BsaJI CCNNGG 6 cut(s) 132, 276, 532, 690, 771, 871
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 277
Bse1I ACTGG 2 cut(s) 211, 827
Bse21I CCTNAGG 1 cut(s) 618
BseBI CCWGG 3 cut(s) 441, 483, 692
BseDI CCNNGG 6 cut(s) 132, 276, 532, 690, 771, 871
BseGI GGATG 4 cut(s) 514, 1105, 1116, 1122
BseLI CCNNNNNNNGG 1 cut(s) 277
BseMII CTCAG 4 cut(s) 89, 209, 609, 615
BseNI ACTGG 2 cut(s) 211, 827
BseRI GAGGAG 2 cut(s) 856, 1166
BseXI GCAGC 1 cut(s) 1143
BseYI CCCAGC 1 cut(s) 1017
BshFI GGCC 1 cut(s) 623
BsiHKCI CYCGRG 3 cut(s) 294, 626, 1022
BslI CCNNNNNNNGG 1 cut(s) 277
BsmAI GTCTC 7 cut(s) 333, 740, 761, 843, 870, 891, 991
BsmI GAATGC 2 cut(s) 272, 402
BsnI GGCC 1 cut(s) 623
Bso31I GGTCTC 2 cut(s) 761, 843
BsoBI CYCGRG 3 cut(s) 294, 626, 1022
Bsp1286I GDGCHC 1 cut(s) 334
Bsp143I GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
Bsp19I CCATGG 1 cut(s) 871
BspACI CCGC 3 cut(s) 614, 910, 1172
BspANI GGCC 1 cut(s) 623
BspCNI CTCAG 4 cut(s) 90, 208, 610, 614
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 1 cut(s) 845
BspPI GGATC 3 cut(s) 552, 934, 1182
BspTNI GGTCTC 2 cut(s) 761, 843
BsrI ACTGG 2 cut(s) 211, 827
BssECI CCNNGG 6 cut(s) 132, 276, 532, 690, 771, 871
BssMI GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
BssSI CACGAG 2 cut(s) 933, 963
BssT1I CCWWGG 3 cut(s) 276, 771, 871
Bst2BI CACGAG 2 cut(s) 933, 963
Bst2UI CCWGG 3 cut(s) 441, 483, 692
Bst4CI ACNGT 2 cut(s) 119, 859
BstC8I GCNNGC 2 cut(s) 303, 363
BstDEI CTNAG 5 cut(s) 98, 195, 461, 601, 618
BstDSI CCRYGG 2 cut(s) 532, 871
BstF5I GGATG 4 cut(s) 514, 1105, 1116, 1122
BstKTI GATC 9 cut(s) 547, 552, 633, 760, 929, 945, 955, 1009, 1177
BstMAI GTCTC 7 cut(s) 333, 740, 761, 843, 870, 891, 991
BstMBI GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
BstMWI GCNNNNNNNGC 2 cut(s) 466, 1018
BstNI CCWGG 3 cut(s) 441, 483, 692
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 3 cut(s) 439, 481, 690
BstV1I GCAGC 1 cut(s) 1143
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 2 cut(s) 549, 1174
BstYI RGATCY 2 cut(s) 549, 1174
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 1 cut(s) 623
BtgI CCRYGG 2 cut(s) 532, 871
BtsCI GGATG 4 cut(s) 514, 1105, 1116, 1122
BtsIMutI CAGTG 1 cut(s) 948
Cac8I GCNNGC 2 cut(s) 303, 363
CaiI CAGNNNCTG 1 cut(s) 863
CciI TCATGA 1 cut(s) 645
Cfr13I GGNCC 3 cut(s) 394, 622, 883
CseI GACGC 1 cut(s) 819
CviAII CATG 6 cut(s) 362, 422, 646, 872, 880, 907
DdeI CTNAG 5 cut(s) 98, 195, 461, 601, 618
DpnI GATC 9 cut(s) 546, 551, 632, 759, 928, 944, 954, 1008, 1176
DpnII GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
Eco130I CCWWGG 3 cut(s) 276, 771, 871
Eco24I GRGCYC 1 cut(s) 334
Eco31I GGTCTC 2 cut(s) 761, 843
Eco47I GGWCC 2 cut(s) 394, 883
Eco81I CCTNAGG 1 cut(s) 618
Eco88I CYCGRG 3 cut(s) 294, 626, 1022
EcoO109I RGGNCCY 1 cut(s) 622
EcoRI GAATTC 1 cut(s) 32
EcoRII CCWGG 3 cut(s) 439, 481, 690
EcoT14I CCWWGG 3 cut(s) 276, 771, 871
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 3 cut(s) 276, 771, 871
FaeI CATG 6 cut(s) 365, 425, 649, 875, 883, 910
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 6 cut(s) 361, 421, 645, 871, 879, 906
FauI CCCGC 1 cut(s) 903
FbaI TGATCA 1 cut(s) 942
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 2 cut(s) 614, 1157
FokI GGATG 4 cut(s) 521, 1109, 1112, 1123
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 2 cut(s) 614, 1157
FspBI CTAG 3 cut(s) 347, 381, 992
GluI GCNGC 2 cut(s) 614, 1157
GsaI CCCAGC 1 cut(s) 1021
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 1 cut(s) 623
HgaI GACGC 1 cut(s) 819
Hin1II CATG 6 cut(s) 365, 425, 649, 875, 883, 910
HincII GTYRAC 3 cut(s) 724, 820, 828
HindII GTYRAC 3 cut(s) 724, 820, 828
HindIII AAGCTT 1 cut(s) 740
HinfI GANTC 6 cut(s) 91, 105, 184, 583, 664, 748
HphI GGTGA 2 cut(s) 679, 937
Hpy166II GTNNAC 4 cut(s) 724, 820, 828, 1055
Hpy188I TCNGA 2 cut(s) 99, 549
Hpy8I GTNNAC 4 cut(s) 724, 820, 828, 1055
Hpy99I CGWCG 1 cut(s) 813
HpyAV CCTTC 9 cut(s) 172, 313, 459, 689, 1043, 1059, 1063, 1090, 1170
HpyCH4III ACNGT 2 cut(s) 119, 859
HpyCH4IV ACGT 2 cut(s) 1057, 1140
HpyCH4V TGCA 4 cut(s) 17, 168, 287, 361
HpyF10VI GCNNNNNNNGC 2 cut(s) 466, 1018
HpyF3I CTNAG 5 cut(s) 98, 195, 461, 601, 618
HpySE526I ACGT 2 cut(s) 1057, 1140
Hsp92II CATG 6 cut(s) 365, 425, 649, 875, 883, 910
Ksp22I TGATCA 1 cut(s) 942
Kzo9I GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
LmnI GCTCC 2 cut(s) 843, 1153
Lsp1109I GCAGC 1 cut(s) 1143
LweI GCATC 3 cut(s) 61, 498, 1101
MaeI CTAG 3 cut(s) 347, 381, 992
MaeII ACGT 2 cut(s) 1057, 1140
MaeIII GTNAC 2 cut(s) 471, 1090
MalI GATC 9 cut(s) 546, 551, 632, 759, 928, 944, 954, 1008, 1176
MboI GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
MboII GAAGA 5 cut(s) 74, 86, 230, 563, 1169
MflI RGATCY 2 cut(s) 549, 1174
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 4 cut(s) 32, 153, 707, 1041
MlyI GAGTC 1 cut(s) 742
MmeI TCCRAC 1 cut(s) 412
MseI TTAA 2 cut(s) 801, 999
MslI CAYNNNNRTG 3 cut(s) 366, 494, 905
MspR9I CCNGG 3 cut(s) 441, 483, 692
Mva1269I GAATGC 2 cut(s) 272, 402
MvaI CCWGG 3 cut(s) 441, 483, 692
MwoI GCNNNNNNNGC 2 cut(s) 466, 1018
NcoI CCATGG 1 cut(s) 871
NdeII GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
NlaIII CATG 6 cut(s) 365, 425, 649, 875, 883, 910
NlaIV GGNNCC 1 cut(s) 845
NmeAIII GCCGAG 2 cut(s) 113, 872
NmuCI GTSAC 1 cut(s) 471
NspI RCATGY 1 cut(s) 365
PaeI GCATGC 1 cut(s) 365
PaeR7I CTCGAG 2 cut(s) 294, 626
PagI TCATGA 1 cut(s) 645
PcsI WCGNNNNNNNCGW 1 cut(s) 1054
PctI GAATGC 2 cut(s) 272, 402
PfeI GAWTC 5 cut(s) 91, 105, 184, 583, 664
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 2 cut(s) 615, 1158
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
Psp6I CCWGG 3 cut(s) 439, 481, 690
PspFI CCCAGC 1 cut(s) 1017
PspGI CCWGG 3 cut(s) 439, 481, 690
PspN4I GGNNCC 1 cut(s) 845
PspPI GGNCC 3 cut(s) 394, 622, 883
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 2 cut(s) 549, 1174
RseI CAYNNNNRTG 3 cut(s) 366, 494, 905
SalI GTCGAC 1 cut(s) 722
SaqAI TTAA 2 cut(s) 801, 999
SatI GCNGC 2 cut(s) 614, 1157
Sau3AI GATC 9 cut(s) 544, 549, 630, 757, 926, 942, 952, 1006, 1174
Sau96I GGNCC 3 cut(s) 394, 622, 883
SchI GAGTC 1 cut(s) 742
ScrFI CCNGG 3 cut(s) 441, 483, 692
SduI GDGCHC 1 cut(s) 334
SfaNI GCATC 3 cut(s) 61, 498, 1101
Sfr274I CTCGAG 2 cut(s) 294, 626
SinI GGWCC 2 cut(s) 394, 883
SlaI CTCGAG 2 cut(s) 294, 626
SmiMI CAYNNNNRTG 3 cut(s) 366, 494, 905
SmlI CTYRAG 3 cut(s) 294, 626, 743
SmoI CTYRAG 3 cut(s) 294, 626, 743
SpeI ACTAGT 1 cut(s) 380
SphI GCATGC 1 cut(s) 365
Sse9I AATT 4 cut(s) 32, 153, 707, 1041
SsiI CCGC 3 cut(s) 614, 910, 1172
SspMI CTAG 3 cut(s) 347, 381, 992
StyD4I CCNGG 3 cut(s) 439, 481, 690
StyI CCWWGG 3 cut(s) 276, 771, 871
TaaI ACNGT 2 cut(s) 119, 859
TaiI ACGT 2 cut(s) 1060, 1143
TaqI TCGA 4 cut(s) 295, 627, 723, 808
TasI AATT 4 cut(s) 32, 153, 707, 1041
TauI GCSGC 1 cut(s) 616
TfiI GAWTC 5 cut(s) 91, 105, 184, 583, 664
Tru1I TTAA 2 cut(s) 801, 999
Tru9I TTAA 2 cut(s) 801, 999
TscAI CASTG 1 cut(s) 955
TseFI GTSAC 1 cut(s) 471
TseI GCWGC 1 cut(s) 1156
Tsp45I GTSAC 1 cut(s) 471
TspDTI ATGAA 6 cut(s) 66, 410, 534, 634, 662, 696
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 1 cut(s) 955
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 2 cut(s) 394, 883
XapI RAATTY 1 cut(s) 32
XceI RCATGY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XhoI CTCGAG 2 cut(s) 294, 626
XmiI GTMKAC 1 cut(s) 723
XspI CTAG 3 cut(s) 347, 381, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.