Rh2DG084800

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000799
Physical Location & Seq
Reverse (-)
6606365 .. 6607356
992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG084800.1

Sequence Viewer

Length: 843 bp
ATGAGTTGTAAGAATACTTTTTTCTGTTTCTGTACTTTATTACATGTTTTGATGTACGAAACACATCTTTTGTTGGGAAGTGATGTCGGCATGTGTGATTCTACTATTGTCTTTTTGTTACGTTCCAATGGGTATTTATATCGTGGTGCTAATGTATGGGGAATTCATTGTTACAGGCTTGCAACTGATGGACAAGATTTCTTTACATCATACGATGAAGTTTACGACAGTTTTGATTCCATGGGCTTGCAAGAGAACCTGCTGAGGGGCATTTATGCTTACGGTTTTGAGAAACCCTCTGCCATTCAGCAAAGGGGAATTGTCCCCTTTATTAAGGGTCTTGATGTGATTCAGCAGGCTCAATCTGGAACTGGGAAGACAGCGACCTTCTGTTCTGGTATTCTTCAACAGCTTGAATATAGTTTGACAGAGTGCCAGGGCTTGGTTCTGGCACCCACTCGAGAGCTTGCCCAACAAATAGAGAAGGTCATGAGGGCCCTTGGAGATTATCTTGGTGTTAAGGTGCATGCATGTGTTGGTGGAACTAGTGTTCGTGAAGACCAACGTATTCTGTCAAATGGAGTTCATGCTGTTGTTGGAACTCCAGGTCGTGTTTTTGATATGCTAAGAAGACAGTCACTTCGCCCTGATCATATCAAGATGTTTGTTCTTGATGAGGCTGATGAAATGCTCTCTCGGGGTTTCAAGGACCAGGTTTGTTTCCAAGACAATTTCTTCATATTATTTATGTGTTTTTATGATCTACGAGTATATCCTGCAGTATTTCATATGCATGAAATGAAAAGAATCATGAGTATGTTAAAAGTCATATTATTGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

280

Amino Acids

31.83

Weight (kDa)

6.23

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 101 - 239 1.5e-35 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 267
AccB1I GGYRCC 1 cut(s) 451
AccB7I CCANNNNNTGG 1 cut(s) 442
AcsI RAATTY 1 cut(s) 162
AfaI GTAC 2 cut(s) 34, 56
AfiI CCNNNNNNNGG 2 cut(s) 265, 442
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 3 cut(s) 407, 416, 706
AhlI ACTAGT 1 cut(s) 545
AjnI CCWGG 3 cut(s) 435, 604, 711
AloI GAACNNNNNNTCC 2 cut(s) 534, 566
AluBI AGCT 2 cut(s) 412, 466
AluI AGCT 2 cut(s) 412, 466
Ama87I CYCGRG 2 cut(s) 459, 696
AoxI GGCC 1 cut(s) 495
ApaI GGGCCC 1 cut(s) 499
ApoI RAATTY 1 cut(s) 162
ArsI GACNNNNNNTTYG 2 cut(s) 624, 656
AspS9I GGNCC 3 cut(s) 495, 496, 709
AvaI CYCGRG 2 cut(s) 459, 696
AvaII GGWCC 1 cut(s) 709
BaeGI GKGCMC 1 cut(s) 499
BanI GGYRCC 1 cut(s) 451
BanII GRGCYC 1 cut(s) 499
BbsI GAAGAC 3 cut(s) 383, 564, 637
BbvCI CCTCAGC 1 cut(s) 263
BccI CCATC 1 cut(s) 182
BciT130I CCWGG 3 cut(s) 437, 606, 713
BclI TGATCA 1 cut(s) 649
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 1 cut(s) 777
BfuAI ACCTGC 1 cut(s) 267
Bme1390I CCNGG 3 cut(s) 437, 606, 713
Bme18I GGWCC 1 cut(s) 709
BmeT110I CYCGRG 2 cut(s) 459, 696
BmgT120I GGNCC 3 cut(s) 495, 496, 709
BmiI GGNNCC 2 cut(s) 453, 497
BmrFI CCNGG 3 cut(s) 437, 606, 713
BmrI ACTGGG 1 cut(s) 381
BmuI ACTGGG 1 cut(s) 381
BpiI GAAGAC 3 cut(s) 383, 564, 637
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpmI CTGGAG 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 240, 436, 499
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 442
Bse1I ACTGG 1 cut(s) 376
BseBI CCWGG 3 cut(s) 437, 606, 713
BseDI CCNNGG 3 cut(s) 240, 436, 499
BseLI CCNNNNNNNGG 2 cut(s) 265, 442
BseMII CTCAG 1 cut(s) 254
BseNI ACTGG 1 cut(s) 376
BseSI GKGCMC 1 cut(s) 499
BshFI GGCC 1 cut(s) 497
BshNI GGYRCC 1 cut(s) 451
BsiHKCI CYCGRG 2 cut(s) 459, 696
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 2 cut(s) 265, 442
BsmFI GGGAC 1 cut(s) 308
BsnI GGCC 1 cut(s) 497
BsoBI CYCGRG 2 cut(s) 459, 696
Bsp120I GGGCCC 1 cut(s) 495
Bsp1286I GDGCHC 1 cut(s) 499
Bsp143I GATC 2 cut(s) 649, 760
Bsp19I CCATGG 1 cut(s) 240
BspANI GGCC 1 cut(s) 497
BspCNI CTCAG 1 cut(s) 255
BspHI TCATGA 2 cut(s) 489, 810
BspLI GGNNCC 2 cut(s) 453, 497
BspMAI CTGCAG 1 cut(s) 781
BspMI ACCTGC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 451
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 3 cut(s) 240, 436, 499
BssMI GATC 2 cut(s) 649, 760
BssT1I CCWWGG 2 cut(s) 240, 499
Bst2UI CCWGG 3 cut(s) 437, 606, 713
Bst4CI ACNGT 3 cut(s) 230, 284, 636
BstC8I GCNNGC 5 cut(s) 180, 248, 357, 468, 528
BstDEI CTNAG 2 cut(s) 263, 626
BstDSI CCRYGG 1 cut(s) 240
BstKTI GATC 2 cut(s) 652, 763
BstMBI GATC 2 cut(s) 649, 760
BstNI CCWGG 3 cut(s) 437, 606, 713
BstNSI RCATGY 4 cut(s) 47, 94, 530, 534
BstSCI CCNGG 3 cut(s) 435, 604, 711
BstSFI CTRYAG 1 cut(s) 777
BstSLI GKGCMC 1 cut(s) 499
BstV2I GAAGAC 3 cut(s) 383, 564, 637
BsuRI GGCC 1 cut(s) 497
BtgI CCRYGG 1 cut(s) 240
BveI ACCTGC 1 cut(s) 267
Cac8I GCNNGC 5 cut(s) 180, 248, 357, 468, 528
CciI TCATGA 2 cut(s) 489, 810
Cfr13I GGNCC 3 cut(s) 495, 496, 709
CsiI ACCWGGT 1 cut(s) 711
Csp6I GTAC 2 cut(s) 33, 55
CviAII CATG 9 cut(s) 44, 91, 241, 490, 527, 531, 587, 794, 811
CviJI RGCY 8 cut(s) 178, 246, 359, 412, 441, 466, 497, 680
CviKI_1 RGCY 8 cut(s) 178, 246, 359, 412, 441, 466, 497, 680
CviQI GTAC 2 cut(s) 33, 55
DdeI CTNAG 2 cut(s) 263, 626
DpnI GATC 2 cut(s) 651, 762
DpnII GATC 2 cut(s) 649, 760
Eco130I CCWWGG 2 cut(s) 240, 499
Eco24I GRGCYC 1 cut(s) 499
Eco47I GGWCC 1 cut(s) 709
Eco88I CYCGRG 2 cut(s) 459, 696
EcoO109I RGGNCCY 2 cut(s) 495, 496
EcoRI GAATTC 1 cut(s) 162
EcoRII CCWGG 3 cut(s) 435, 604, 711
EcoT14I CCWWGG 2 cut(s) 240, 499
EcoT22I ATGCAT 2 cut(s) 532, 795
EcoT38I GRGCYC 1 cut(s) 499
ErhI CCWWGG 2 cut(s) 240, 499
FaeI CATG 9 cut(s) 47, 94, 244, 493, 530, 534, 590, 797, 814
FaqI GGGAC 1 cut(s) 308
FatI CATG 9 cut(s) 43, 90, 240, 489, 526, 530, 586, 793, 810
FauNDI CATATG 1 cut(s) 789
FbaI TGATCA 1 cut(s) 649
FriOI GRGCYC 1 cut(s) 499
FspBI CTAG 1 cut(s) 546
GsuI CTGGAG 1 cut(s) 588
HaeIII GGCC 1 cut(s) 497
Hin1II CATG 9 cut(s) 47, 94, 244, 493, 530, 534, 590, 797, 814
HinfI GANTC 4 cut(s) 98, 236, 349, 807
Hpy166II GTNNAC 1 cut(s) 223
Hpy188III TCNNGA 8 cut(s) 341, 366, 461, 490, 554, 658, 671, 811
Hpy8I GTNNAC 1 cut(s) 223
HpyAV CCTTC 2 cut(s) 397, 478
HpyCH4III ACNGT 3 cut(s) 230, 284, 636
HpyCH4IV ACGT 2 cut(s) 121, 565
HpyCH4V TGCA 6 cut(s) 182, 250, 526, 530, 779, 793
HpyF3I CTNAG 2 cut(s) 263, 626
HpySE526I ACGT 2 cut(s) 121, 565
Hsp92II CATG 9 cut(s) 47, 94, 244, 493, 530, 534, 590, 797, 814
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 2 cut(s) 649, 760
MabI ACCWGGT 1 cut(s) 711
MaeI CTAG 1 cut(s) 546
MaeII ACGT 2 cut(s) 121, 565
MaeIII GTNAC 3 cut(s) 117, 170, 636
MalI GATC 2 cut(s) 651, 762
MboI GATC 2 cut(s) 649, 760
MboII GAAGA 5 cut(s) 388, 395, 569, 642, 727
MhlI GDGCHC 1 cut(s) 499
MluCI AATT 3 cut(s) 162, 318, 730
MmeI TCCRAC 1 cut(s) 577
MnlI CCTC 4 cut(s) 258, 307, 486, 670
Mph1103I ATGCAT 2 cut(s) 532, 795
MseI TTAA 3 cut(s) 333, 519, 821
MslI CAYNNNNRTG 3 cut(s) 531, 792, 815
MspR9I CCNGG 3 cut(s) 437, 606, 713
MvaI CCWGG 3 cut(s) 437, 606, 713
NcoI CCATGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 789
NdeII GATC 2 cut(s) 649, 760
NlaIII CATG 9 cut(s) 47, 94, 244, 493, 530, 534, 590, 797, 814
NlaIV GGNNCC 2 cut(s) 453, 497
NmuCI GTSAC 1 cut(s) 636
NsiI ATGCAT 2 cut(s) 532, 795
NspI RCATGY 4 cut(s) 47, 94, 530, 534
PaeI GCATGC 1 cut(s) 530
PaeR7I CTCGAG 1 cut(s) 459
PagI TCATGA 2 cut(s) 489, 810
PciI ACATGT 1 cut(s) 43
PfeI GAWTC 4 cut(s) 98, 236, 349, 807
PflMI CCANNNNNTGG 1 cut(s) 442
PscI ACATGT 1 cut(s) 43
Psp6I CCWGG 3 cut(s) 435, 604, 711
PspGI CCWGG 3 cut(s) 435, 604, 711
PspN4I GGNNCC 2 cut(s) 453, 497
PspOMI GGGCCC 1 cut(s) 495
PspPI GGNCC 3 cut(s) 495, 496, 709
PstI CTGCAG 1 cut(s) 781
RsaI GTAC 2 cut(s) 34, 56
RsaNI GTAC 2 cut(s) 33, 55
RseI CAYNNNNRTG 3 cut(s) 531, 792, 815
SaqAI TTAA 3 cut(s) 333, 519, 821
Sau3AI GATC 2 cut(s) 649, 760
Sau96I GGNCC 3 cut(s) 495, 496, 709
ScrFI CCNGG 3 cut(s) 437, 606, 713
SduI GDGCHC 1 cut(s) 499
SexAI ACCWGGT 1 cut(s) 711
SfcI CTRYAG 1 cut(s) 777
Sfr274I CTCGAG 1 cut(s) 459
SinI GGWCC 1 cut(s) 709
SlaI CTCGAG 1 cut(s) 459
SmiMI CAYNNNNRTG 3 cut(s) 531, 792, 815
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
SpeI ACTAGT 1 cut(s) 545
SphI GCATGC 1 cut(s) 530
Sse9I AATT 3 cut(s) 162, 318, 730
SspMI CTAG 1 cut(s) 546
StyD4I CCNGG 3 cut(s) 435, 604, 711
StyI CCWWGG 2 cut(s) 240, 499
TaaI ACNGT 3 cut(s) 230, 284, 636
TaiI ACGT 2 cut(s) 124, 568
TaqI TCGA 1 cut(s) 460
TasI AATT 3 cut(s) 162, 318, 730
TatI WGTACW 1 cut(s) 32
TfiI GAWTC 4 cut(s) 98, 236, 349, 807
Tru1I TTAA 3 cut(s) 333, 519, 821
Tru9I TTAA 3 cut(s) 333, 519, 821
TseFI GTSAC 1 cut(s) 636
Tsp45I GTSAC 1 cut(s) 636
TspDTI ATGAA 8 cut(s) 155, 231, 575, 699, 727, 776, 810, 815
Van91I CCANNNNNTGG 1 cut(s) 442
VpaK11BI GGWCC 1 cut(s) 709
XapI RAATTY 1 cut(s) 162
XceI RCATGY 4 cut(s) 47, 94, 530, 534
XhoI CTCGAG 1 cut(s) 459
XspI CTAG 1 cut(s) 546
Zsp2I ATGCAT 2 cut(s) 532, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.