Prupe.3G045500_v2.0.a1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000799
Physical Location & Seq
Forward (+)
3270174 .. 3273732
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G045500.1

Sequence Viewer

Length: 1242 bp
ATGGCAGGTGTTGCACCCGAAGGATCACAGTTTGATGCAAAGCAATATGACACTAAAATGAGTGAATTGCTCTCAACTGATGGACAAGAGTTTTTCACATCATATGATGAAGTTTATGAAAGTTTTGATTCCATGGGGTTGCAAGAAAATCTTCTGAGGGGAATTTATGCTTACGGTTTTGAGAAGCCTTCTGCAATTCAGCAAAGGGGAATTGTTCCATTCTGCAAAGGCCTCGATGTGATTCAACAGGCTCAGTCTGGAACCGGAAAAACTGCAACCTTCTGCTCTGGAATTTTGCAGCAACTTGATTATGCTGTAGTCCAGTGCCAGGCTTTGGTTTTGGCACCTACCAGGGAGTTGGCACAGCAGATTGAGAAGGTTATGCGAGCACTTGGTGACTATCTTGGCGTGAAGGTTCATGCCTGTGTAGGCGGGACAAGTGTTCGTGAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTTGTTGTTGGAACCCCTGGTCGTGTATTTGACATGTTGCGGAGGCAGTCTCTTCGTCCAGATTACATCAAGATGTTTGTGTTGGATGAGGCCGATGAAATGCTTTCTCGGGGTTTTAAGGACCAGATCTATGACATTTTCCAGCTTCTGCCATCAAAAGTTCAGGTTGGGGTGTTCTCTGCTACAATGCCACCTGAAGCACTTGAAATCACGAGGAAGTTTATGAACAAACCTGTGAGGATCTTGGTGAAGCGTGATGAGCTCACCCTTGAGGGTATCAAGCAATTTTATGTCAATGTTGATAAAGAAGAATGGAAGCTTGAGACACTTTGTGATCTGTATGAGACCCTGGCCATCACCCAGAGTGTCATTTTTGTTAATACACGGCGAAAGGTGGACTGGCTTACTGACAAGATGCGAAGCCGTGACCACACTGTCTCAGCTACTCATGGTGACATGGACCAGAACACTCGTGACATTATCATGCGTGAATTCCGCTCTGGCTCGTCCCGTGTTCTCATAACCACTGATCTTTTGGCTCGTGGTATTGATGTGCAGCAGGTATCTCTCGTCATAAATTATGATTTGCCGACCCAACCAGAGAATTACCTTCATCGTATTGGTCGAAGTGGAAGGTTTGGAAGGAAGGGTGTTGCCATTAACTTTGTGACTAGGGATGACGAGAGAATGCTCTATGACATTCAGAGGTTTTACAATGTGGTCATTGAGGAGCTGCCATCAAATGTCGCTGATCTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

414

Amino Acids

46.94

Weight (kDa)

5.48

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 914
Acc36I ACCTGC 1 cut(s) 1030
AccB1I GGYRCC 1 cut(s) 343
AccB7I CCANNNNNTGG 1 cut(s) 334
AccBSI CCGCTC 1 cut(s) 978
AciI CCGC 3 cut(s) 432, 520, 976
AclWI GGATC 3 cut(s) 31, 459, 728
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 3 cut(s) 162, 291, 971
AcuI CTGAAG 1 cut(s) 696
AdeI CACNNNGTG 2 cut(s) 395, 812
AfiI CCNNNNNNNGG 2 cut(s) 328, 334
AflIII ACRYGT 1 cut(s) 513
AgsI TTSAA 3 cut(s) 245, 467, 686
AjnI CCWGG 4 cut(s) 327, 350, 496, 828
AluBI AGCT 6 cut(s) 470, 625, 742, 799, 923, 1213
AluI AGCT 6 cut(s) 470, 625, 742, 799, 923, 1213
Alw21I GWGCWC 2 cut(s) 391, 744
Alw26I GTCTC 4 cut(s) 534, 797, 818, 922
AlwI GGATC 3 cut(s) 31, 459, 728
AlwNI CAGNNNCTG 1 cut(s) 628
Ama87I CYCGRG 1 cut(s) 588
AoxI GGCC 3 cut(s) 229, 570, 831
ApeKI GCWGC 3 cut(s) 298, 1036, 1213
ApoI RAATTY 3 cut(s) 162, 291, 971
Asp700I GAANNNNTTC 1 cut(s) 150
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 2 cut(s) 601, 940
AsuHPI GGTGA 5 cut(s) 407, 736, 739, 829, 944
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 2 cut(s) 601, 940
BalI TGGCCA 1 cut(s) 833
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 744
BauI CACGAG 3 cut(s) 691, 951, 1020
Bbv12I GWGCWC 2 cut(s) 391, 744
BbvI GCAGC 3 cut(s) 310, 1048, 1200
BccI CCATC 4 cut(s) 74, 640, 842, 1225
BceAI ACGGC 2 cut(s) 881, 888
BcgI CGANNNNNNTGC 4 cut(s) 214, 248, 515, 549
BciT130I CCWGG 4 cut(s) 329, 352, 498, 830
BcoDI GTCTC 4 cut(s) 534, 797, 818, 922
BfaI CTAG 1 cut(s) 1152
BfmI CTRYAG 1 cut(s) 315
BfuAI ACCTGC 1 cut(s) 1030
BglII AGATCT 1 cut(s) 606
BisI GCNGC 3 cut(s) 299, 1037, 1214
BlsI GCNGC 3 cut(s) 300, 1038, 1215
Bme1390I CCNGG 4 cut(s) 329, 352, 498, 830
Bme18I GGWCC 2 cut(s) 601, 940
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 601, 940
BmiI GGNNCC 3 cut(s) 262, 345, 493
BmrFI CCNGG 4 cut(s) 329, 352, 498, 830
BmsI GCATC 2 cut(s) 25, 885
BplI GAGNNNNNCTC 2 cut(s) 514, 546
BpuEI CTTGAG 2 cut(s) 770, 821
BsaI GGTCTC 1 cut(s) 818
BsaJI CCNNGG 4 cut(s) 132, 351, 496, 828
BsaWI WCCGGW 1 cut(s) 263
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 334
Bse1I ACTGG 2 cut(s) 322, 884
BseBI CCWGG 4 cut(s) 329, 352, 498, 830
BseDI CCNNGG 4 cut(s) 132, 351, 496, 828
BseGI GGATG 2 cut(s) 571, 1162
BseLI CCNNNNNNNGG 2 cut(s) 328, 334
BseMII CTCAG 3 cut(s) 146, 266, 933
BseNI ACTGG 2 cut(s) 322, 884
BseRI GAGGAG 1 cut(s) 1223
BseXI GCAGC 3 cut(s) 310, 1048, 1200
BsgI GTGCAG 1 cut(s) 1055
BshFI GGCC 3 cut(s) 231, 572, 833
BshNI GGYRCC 1 cut(s) 343
BsiHKAI GWGCWC 2 cut(s) 391, 744
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 2 cut(s) 448, 973
BslI CCNNNNNNNGG 2 cut(s) 328, 334
BsmAI GTCTC 4 cut(s) 534, 797, 818, 922
BsmFI GGGAC 2 cut(s) 448, 973
BsmI GAATGC 2 cut(s) 459, 1173
BsnI GGCC 3 cut(s) 231, 572, 833
Bso31I GGTCTC 1 cut(s) 818
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 2 cut(s) 391, 744
Bsp143I GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 3 cut(s) 432, 520, 976
BspANI GGCC 3 cut(s) 231, 572, 833
BspCNI CTCAG 3 cut(s) 147, 265, 932
BspLI GGNNCC 3 cut(s) 262, 345, 493
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 3 cut(s) 31, 459, 728
BspT107I GGYRCC 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 818
BsrBI CCGCTC 1 cut(s) 978
BsrI ACTGG 2 cut(s) 322, 884
BssECI CCNNGG 4 cut(s) 132, 351, 496, 828
BssMI GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
BssSI CACGAG 3 cut(s) 691, 951, 1020
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 3 cut(s) 691, 951, 1020
Bst2UI CCWGG 4 cut(s) 329, 352, 498, 830
Bst4CI ACNGT 3 cut(s) 30, 176, 916
Bst6I CTCTTC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 3 cut(s) 155, 252, 919
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 571, 1162
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 7 cut(s) 26, 454, 609, 723, 817, 1012, 1234
BstMAI GTCTC 4 cut(s) 534, 797, 818, 922
BstMBI GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 11, 739
BstNI CCWGG 4 cut(s) 329, 352, 498, 830
BstNSI RCATGY 1 cut(s) 517
BstSCI CCNGG 4 cut(s) 327, 350, 496, 828
BstSFI CTRYAG 1 cut(s) 315
BstV1I GCAGC 3 cut(s) 310, 1048, 1200
BstX2I RGATCY 2 cut(s) 606, 720
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 2 cut(s) 606, 720
BsuRI GGCC 3 cut(s) 231, 572, 833
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 571, 1162
BtsIMutI CAGTG 3 cut(s) 329, 912, 1005
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 628
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 2 cut(s) 601, 940
CviAII CATG 7 cut(s) 133, 419, 479, 514, 929, 937, 964
DdeI CTNAG 3 cut(s) 155, 252, 919
DpnI GATC 7 cut(s) 25, 453, 608, 722, 816, 1011, 1233
DpnII GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
DraIII CACNNNGTG 2 cut(s) 395, 812
DrdI GACNNNNNNGTC 1 cut(s) 914
DseDI GACNNNNNNGTC 1 cut(s) 914
EaeI YGGCCR 1 cut(s) 831
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 1 cut(s) 132
Eco147I AGGCCT 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 818
Eco47I GGWCC 2 cut(s) 601, 940
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 588
EcoICRI GAGCTC 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 971
EcoRII CCWGG 4 cut(s) 327, 350, 496, 828
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 2 cut(s) 448, 973
FatI CATG 7 cut(s) 132, 418, 478, 513, 928, 936, 963
FauI CCCGC 1 cut(s) 425
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 299, 1037, 1214
FokI GGATG 2 cut(s) 578, 1169
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 3 cut(s) 299, 1037, 1214
FspBI CTAG 1 cut(s) 1152
GlaI GCGC 1 cut(s) 458
GluI GCNGC 3 cut(s) 299, 1037, 1214
HaeIII GGCC 3 cut(s) 231, 572, 833
HapII CCGG 1 cut(s) 264
HhaI GCGC 1 cut(s) 459
Hin1II CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 2 cut(s) 128, 241
HpaII CCGG 1 cut(s) 264
HphI GGTGA 5 cut(s) 407, 736, 739, 829, 944
Hpy166II GTNNAC 1 cut(s) 877
Hpy188I TCNGA 3 cut(s) 156, 1185, 1241
Hpy188III TCNNGA 7 cut(s) 258, 288, 446, 539, 550, 691, 953
Hpy8I GTNNAC 1 cut(s) 877
HpyAV CCTTC 9 cut(s) 14, 198, 289, 370, 406, 1100, 1107, 1116, 1120
HpyCH4III ACNGT 3 cut(s) 30, 176, 916
HpyCH4V TGCA 8 cut(s) 14, 38, 142, 194, 225, 275, 298, 1036
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 739
HpyF3I CTNAG 3 cut(s) 155, 252, 919
Hsp92II CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
HspAI GCGC 1 cut(s) 457
Kzo9I GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
LmnI GCTCC 1 cut(s) 1210
Lsp1109I GCAGC 3 cut(s) 310, 1048, 1200
LweI GCATC 2 cut(s) 25, 885
MaeI CTAG 1 cut(s) 1152
MaeIII GTNAC 5 cut(s) 395, 905, 932, 953, 1147
MalI GATC 7 cut(s) 25, 453, 608, 722, 816, 1011, 1233
MbiI CCGCTC 1 cut(s) 978
MboI GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
MboII GAAGA 4 cut(s) 143, 455, 524, 800
MflI RGATCY 2 cut(s) 606, 720
MhlI GDGCHC 2 cut(s) 391, 744
MlsI TGGCCA 1 cut(s) 833
MluCI AATT 9 cut(s) 65, 162, 195, 210, 291, 764, 971, 1057, 1084
MluNI TGGCCA 1 cut(s) 833
MmeI TCCRAC 2 cut(s) 469, 543
Mox20I TGGCCA 1 cut(s) 833
MroXI GAANNNNTTC 1 cut(s) 150
MscI TGGCCA 1 cut(s) 833
MseI TTAA 3 cut(s) 597, 858, 1140
MslI CAYNNNNRTG 4 cut(s) 56, 423, 551, 962
Msp20I TGGCCA 1 cut(s) 833
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 4 cut(s) 329, 352, 498, 830
Mva1269I GAATGC 2 cut(s) 459, 1173
MvaI CCWGG 4 cut(s) 329, 352, 498, 830
MwoI GCNNNNNNNGC 2 cut(s) 11, 739
NcoI CCATGG 1 cut(s) 132
NdeI CATATG 1 cut(s) 103
NdeII GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
NlaIII CATG 7 cut(s) 136, 422, 482, 517, 932, 940, 967
NlaIV GGNNCC 3 cut(s) 262, 345, 493
NmuCI GTSAC 5 cut(s) 395, 905, 932, 953, 1147
NspI RCATGY 1 cut(s) 517
PceI AGGCCT 1 cut(s) 231
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 1102
PctI GAATGC 2 cut(s) 459, 1173
PdmI GAANNNNTTC 1 cut(s) 150
PfeI GAWTC 2 cut(s) 128, 241
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 3 cut(s) 300, 1038, 1215
PscI ACATGT 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 4 cut(s) 327, 350, 496, 828
PspGI CCWGG 4 cut(s) 327, 350, 496, 828
PspN4I GGNNCC 3 cut(s) 262, 345, 493
PspPI GGNCC 2 cut(s) 601, 940
PstNI CAGNNNCTG 1 cut(s) 628
PsuI RGATCY 2 cut(s) 606, 720
RseI CAYNNNNRTG 4 cut(s) 56, 423, 551, 962
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 3 cut(s) 597, 858, 1140
SatI GCNGC 3 cut(s) 299, 1037, 1214
Sau3AI GATC 7 cut(s) 23, 451, 606, 720, 814, 1009, 1231
Sau96I GGNCC 2 cut(s) 601, 940
ScrFI CCNGG 4 cut(s) 329, 352, 498, 830
SduI GDGCHC 2 cut(s) 391, 744
SfaNI GCATC 2 cut(s) 25, 885
SfcI CTRYAG 1 cut(s) 315
SinI GGWCC 2 cut(s) 601, 940
SmiMI CAYNNNNRTG 4 cut(s) 56, 423, 551, 962
SmlI CTYRAG 2 cut(s) 749, 800
SmoI CTYRAG 2 cut(s) 749, 800
Sse9I AATT 9 cut(s) 65, 162, 195, 210, 291, 764, 971, 1057, 1084
SseBI AGGCCT 1 cut(s) 231
SsiI CCGC 3 cut(s) 432, 520, 976
SspMI CTAG 1 cut(s) 1152
SstI GAGCTC 1 cut(s) 744
StuI AGGCCT 1 cut(s) 231
StyD4I CCNGG 4 cut(s) 327, 350, 496, 828
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 30, 176, 916
TaqI TCGA 2 cut(s) 234, 1105
TasI AATT 9 cut(s) 65, 162, 195, 210, 291, 764, 971, 1057, 1084
TfiI GAWTC 2 cut(s) 128, 241
Tru1I TTAA 3 cut(s) 597, 858, 1140
Tru9I TTAA 3 cut(s) 597, 858, 1140
TscAI CASTG 3 cut(s) 329, 919, 1012
TseFI GTSAC 5 cut(s) 395, 905, 932, 953, 1147
TseI GCWGC 3 cut(s) 298, 1036, 1213
Tsp45I GTSAC 5 cut(s) 395, 905, 932, 953, 1147
TspDTI ATGAA 7 cut(s) 123, 132, 407, 467, 591, 719, 1082
TspRI CASTG 3 cut(s) 329, 919, 1012
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 601, 940
XapI RAATTY 3 cut(s) 162, 291, 971
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 1012
XmnI GAANNNNTTC 1 cut(s) 150
XspI CTAG 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.