Rh2CG425800

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000799
Physical Location & Seq
Reverse (-)
57946996 .. 57949624
2629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG425800.1

Sequence Viewer

Length: 1389 bp
ATGTTTTCCCATATATTGAGTGATAAGGGCAAGGGCATCCGAGGAGTTTGGCAAACCCTAGCTCTCTCTCTTAGCTCTCTACCTATTCTTCAGCTCTCTATCTCTCTTTGCTTTTCTCCGTCAAGCTTTTCATCAACTCAATCAGATATGGCAGGTCTTGCTCCCGAAGGTGCACAGTTTGATGCTAAACAGTATGATTCTAAGATGAATGAGATCCTCTCATCTGAAGGCCAAGAGTTCTTCACATCGTATGATGAGGTTCATGACAGTTTTGATGCCATGGGTTTACAAGAAAATCTTTTAAGGGGAATATATGCTTATGGATTTGAGAAGCCTTCTGCGATTCAGCAAAGAGGAATTGTTCCTTTCTGTAAAGGTTTGGATGTGATTCAACAAGCTCAGTCAGGAACAGGCAAAACAGCAACCTTTTGCTCGGGAATTTTGCAGCAACTTGACTATGGTTTGGTCCAGTGCCAGGCTTTGGTGTTGGCACCTACCAGGGAGTTAGCTCAGCAGATTGAGAAGGTTATGCGGGCACTTGGAGACTATCTCGGCGTGAAGGTTCATGCTTGCGTAGGTGGGACTAGTGTTCGTGAGGATCAGCGAATTCTTCAGACTGGAGTGCATGTGGTGGTTGGCACTCCTGGACGTGTGTTTGACATGTTGCGGAGGCAATCTCTTCGTGCAGATTACATTAAGATGTTTGTATTGGATGAAGCCGATGAAATGCTATCTAGGGGTTTCAAGGATCAGATCTATGACATTTTTCAGCTTCTGCCTTCAAAAGTTCAAGTTGGTGTGTTCTCTGCTACAATGCCTCCTGAAGCCCTTGAGATCACCAGAAAGTTTATGAACAAACCTGTTAGGATTTTGGTTAAGCGTGATGAGCTCACCCTTGAGGGAATCAAGCAATTTTATGTTAATGTCGATAAGGAAGAATGGAAGCTTGAGACACTTTGCGATCTCTATGAGACCCTTGCAATCACTCAGAGTGTCATTTTTGTTAATACAAGGCGCAAAGTGGATTGGCTTACAGATAAGATGCGAAGCCGTGACCACACTGTGTCTGCTACCCATGGTGATATGGACCAGAACCAGCGTGACATTATCATGCGTGAATTCCGATCTGGTTCCTCCCGTGTTCTCATCACAACTGATCTGTTGGCACGTGGTATTGATGTGCAGCAAGTGTCGCTTGTAATTAATTATGATTTGCCGACTCAACCAGAAAACTACCTTCATCGTATTGGACGAAGTGGGCGATTTGGAAGAAAAGGTGTTGCCATCAATTTCGTGACTAGGGATGACGATAGAATGCTATATGACATTCAGAGGTTTTATAATGTGGTGATTGAGGAGCTGCCTTCCAACGTGGCTGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

462

Amino Acids

52.16

Weight (kDa)

5.77

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 114 - 276 2.1e-43 DEAD/DEAH box helicase
ResIII PF04851 132 - 272 1e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 315 - 423 3.6e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1341
Acc36I ACCTGC 1 cut(s) 143
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 481
AciI CCGC 2 cut(s) 532, 667
AclWI GGATC 3 cut(s) 208, 606, 756
AcsI RAATTY 3 cut(s) 438, 606, 1118
AcuI CTGAAG 4 cut(s) 74, 246, 596, 843
AcvI CACGTG 1 cut(s) 1169
AdeI CACNNNGTG 1 cut(s) 1063
AfiI CCNNNNNNNGG 2 cut(s) 475, 481
AflIII ACRYGT 2 cut(s) 649, 660
AgsI TTSAA 4 cut(s) 392, 745, 783, 791
AhlI ACTAGT 1 cut(s) 584
AjiI CACGTC 1 cut(s) 650
AjnI CCWGG 3 cut(s) 474, 497, 643
Alw21I GWGCWC 2 cut(s) 175, 891
Alw26I GTCTC 3 cut(s) 537, 944, 965
Alw44I GTGCAC 1 cut(s) 171
AlwI GGATC 3 cut(s) 208, 606, 756
AlwNI CAGNNNCTG 1 cut(s) 775
Ama87I CYCGRG 1 cut(s) 433
AoxI GGCC 1 cut(s) 229
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 3 cut(s) 445, 1183, 1360
ApoI RAATTY 3 cut(s) 438, 606, 1118
AseI ATTAAT 1 cut(s) 1203
AspLEI GCGC 1 cut(s) 1017
AspS9I GGNCC 2 cut(s) 466, 1087
AsuHPI GGTGA 4 cut(s) 829, 883, 1091, 1360
AvaI CYCGRG 1 cut(s) 433
AvaII GGWCC 2 cut(s) 466, 1087
BaeGI GKGCMC 2 cut(s) 175, 538
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 1 cut(s) 891
BbrPI CACGTG 1 cut(s) 1169
Bbv12I GWGCWC 2 cut(s) 175, 891
BbvI GCAGC 3 cut(s) 457, 1195, 1347
BccI CCATC 1 cut(s) 1292
BceAI ACGGC 1 cut(s) 1035
BcgI CGANNNNNNTGC 2 cut(s) 662, 696
BciT130I CCWGG 3 cut(s) 476, 499, 645
BcoDI GTCTC 3 cut(s) 537, 944, 965
BcuI ACTAGT 1 cut(s) 584
BfaI CTAG 4 cut(s) 59, 585, 735, 1299
BfuAI ACCTGC 1 cut(s) 143
BglII AGATCT 1 cut(s) 753
BisI GCNGC 3 cut(s) 446, 1184, 1361
BlpI GCTNAGC 1 cut(s) 510
BlsI GCNGC 3 cut(s) 447, 1185, 1362
Bme1390I CCNGG 3 cut(s) 476, 499, 645
Bme18I GGWCC 2 cut(s) 466, 1087
BmeT110I CYCGRG 1 cut(s) 433
BmgBI CACGTC 1 cut(s) 650
BmgT120I GGNCC 2 cut(s) 466, 1087
BmiI GGNNCC 2 cut(s) 492, 1132
BmrFI CCNGG 3 cut(s) 476, 499, 645
BmsI GCATC 4 cut(s) 45, 172, 265, 1032
BplI GAGNNNNNCTC 6 cut(s) 203, 235, 534, 566, 661, 693
BpmI CTGGAG 1 cut(s) 639
Bpu1102I GCTNAGC 1 cut(s) 510
BpuEI CTTGAG 3 cut(s) 851, 917, 968
BsaAI YACGTR 1 cut(s) 1169
BsaI GGTCTC 1 cut(s) 965
BsaJI CCNNGG 4 cut(s) 40, 279, 498, 1075
BsaXI ACNNNNNCTCC 4 cut(s) 612, 642, 802, 832
Bsc4I CCNNNNNNNGG 2 cut(s) 475, 481
Bse1I ACTGG 2 cut(s) 469, 622
BseBI CCWGG 3 cut(s) 476, 499, 645
BseDI CCNNGG 4 cut(s) 40, 279, 498, 1075
BseGI GGATG 4 cut(s) 36, 388, 718, 1309
BseLI CCNNNNNNNGG 2 cut(s) 475, 481
BseMII CTCAG 3 cut(s) 413, 524, 1001
BseNI ACTGG 2 cut(s) 469, 622
BseRI GAGGAG 2 cut(s) 57, 1370
BseSI GKGCMC 2 cut(s) 175, 538
BseXI GCAGC 3 cut(s) 457, 1195, 1347
BsgI GTGCAG 2 cut(s) 705, 1202
BshFI GGCC 1 cut(s) 231
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 2 cut(s) 175, 891
BsiHKCI CYCGRG 1 cut(s) 433
BslFI GGGAC 1 cut(s) 595
BslI CCNNNNNNNGG 2 cut(s) 475, 481
BsmAI GTCTC 3 cut(s) 537, 944, 965
BsmFI GGGAC 1 cut(s) 595
BsmI GAATGC 1 cut(s) 1320
BsnI GGCC 1 cut(s) 231
Bso31I GGTCTC 1 cut(s) 965
BsoBI CYCGRG 1 cut(s) 433
Bsp1286I GDGCHC 3 cut(s) 175, 538, 891
Bsp143I GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
Bsp1720I GCTNAGC 1 cut(s) 510
Bsp19I CCATGG 2 cut(s) 279, 1075
BspACI CCGC 2 cut(s) 532, 667
BspANI GGCC 1 cut(s) 231
BspCNI CTCAG 3 cut(s) 412, 523, 1000
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 492, 1132
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 3 cut(s) 208, 606, 756
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 965
BsrI ACTGG 2 cut(s) 469, 622
BssECI CCNNGG 4 cut(s) 40, 279, 498, 1075
BssMI GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
BssT1I CCWWGG 2 cut(s) 279, 1075
Bst2UI CCWGG 3 cut(s) 476, 499, 645
Bst4CI ACNGT 4 cut(s) 177, 192, 269, 1063
Bst6I CTCTTC 1 cut(s) 684
BstAPI GCANNNNNTGC 1 cut(s) 158
BstBAI YACGTR 1 cut(s) 1169
BstC8I GCNNGC 2 cut(s) 534, 571
BstDEI CTNAG 5 cut(s) 71, 201, 399, 510, 987
BstDSI CCRYGG 2 cut(s) 279, 1075
BstF5I GGATG 4 cut(s) 36, 388, 718, 1309
BstHHI GCGC 1 cut(s) 1017
BstKTI GATC 9 cut(s) 216, 601, 751, 756, 837, 964, 1127, 1159, 1381
BstMAI GTCTC 3 cut(s) 537, 944, 965
BstMBI GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
BstMWI GCNNNNNNNGC 3 cut(s) 158, 886, 1192
BstNI CCWGG 3 cut(s) 476, 499, 645
BstNSI RCATGY 2 cut(s) 629, 664
BstSCI CCNGG 3 cut(s) 474, 497, 643
BstSLI GKGCMC 2 cut(s) 175, 538
BstV1I GCAGC 3 cut(s) 457, 1195, 1347
BstX2I RGATCY 2 cut(s) 213, 753
BstYI RGATCY 2 cut(s) 213, 753
BsuRI GGCC 1 cut(s) 231
BtgI CCRYGG 2 cut(s) 279, 1075
BtrI CACGTC 1 cut(s) 650
BtsCI GGATG 4 cut(s) 36, 388, 718, 1309
BtsIMutI CAGTG 2 cut(s) 476, 1059
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 534, 571
CaiI CAGNNNCTG 1 cut(s) 775
CciI TCATGA 1 cut(s) 262
CfoI GCGC 1 cut(s) 1017
Cfr13I GGNCC 2 cut(s) 466, 1087
CviAII CATG 7 cut(s) 263, 280, 566, 626, 661, 1076, 1111
DdeI CTNAG 5 cut(s) 71, 201, 399, 510, 987
DpnI GATC 9 cut(s) 215, 600, 750, 755, 836, 963, 1126, 1158, 1380
DpnII GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
DraIII CACNNNGTG 1 cut(s) 1063
Eam1104I CTCTTC 1 cut(s) 684
EarI CTCTTC 1 cut(s) 684
Ecl136II GAGCTC 1 cut(s) 889
Eco130I CCWWGG 2 cut(s) 279, 1075
Eco24I GRGCYC 1 cut(s) 891
Eco31I GGTCTC 1 cut(s) 965
Eco47I GGWCC 2 cut(s) 466, 1087
Eco53kI GAGCTC 1 cut(s) 889
Eco57I CTGAAG 4 cut(s) 74, 246, 596, 843
Eco72I CACGTG 1 cut(s) 1169
Eco88I CYCGRG 1 cut(s) 433
EcoICRI GAGCTC 1 cut(s) 889
EcoRI GAATTC 2 cut(s) 606, 1118
EcoRII CCWGG 3 cut(s) 474, 497, 643
EcoT14I CCWWGG 2 cut(s) 279, 1075
EcoT38I GRGCYC 1 cut(s) 891
ErhI CCWWGG 2 cut(s) 279, 1075
FaeI CATG 7 cut(s) 266, 283, 569, 629, 664, 1079, 1114
FalI AAGNNNNNCTT 4 cut(s) 282, 314, 1179, 1211
FaqI GGGAC 1 cut(s) 595
FatI CATG 7 cut(s) 262, 279, 565, 625, 660, 1075, 1110
FauI CCCGC 1 cut(s) 525
Fnu4HI GCNGC 3 cut(s) 446, 1184, 1361
FokI GGATG 4 cut(s) 23, 395, 725, 1316
FriOI GRGCYC 1 cut(s) 891
Fsp4HI GCNGC 3 cut(s) 446, 1184, 1361
FspBI CTAG 4 cut(s) 59, 585, 735, 1299
GlaI GCGC 1 cut(s) 1016
GluI GCNGC 3 cut(s) 446, 1184, 1361
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 1 cut(s) 231
HhaI GCGC 1 cut(s) 1017
Hin1II CATG 7 cut(s) 266, 283, 569, 629, 664, 1079, 1114
Hin6I GCGC 1 cut(s) 1015
HinP1I GCGC 1 cut(s) 1015
HindIII AAGCTT 2 cut(s) 124, 944
HinfI GANTC 5 cut(s) 197, 343, 388, 903, 1219
HphI GGTGA 4 cut(s) 829, 883, 1091, 1360
Hpy166II GTNNAC 2 cut(s) 173, 287
Hpy188I TCNGA 8 cut(s) 41, 145, 226, 615, 753, 990, 1124, 1332
Hpy188III TCNNGA 7 cut(s) 164, 263, 405, 435, 593, 821, 1294
Hpy8I GTNNAC 2 cut(s) 173, 287
HpyAV CCTTC 8 cut(s) 161, 221, 345, 517, 553, 789, 1247, 1374
HpyCH4III ACNGT 4 cut(s) 177, 192, 269, 1063
HpyCH4IV ACGT 3 cut(s) 649, 1168, 1371
HpyCH4V TGCA 6 cut(s) 173, 445, 625, 686, 980, 1183
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 886, 1192
HpyF3I CTNAG 5 cut(s) 71, 201, 399, 510, 987
HpySE526I ACGT 3 cut(s) 649, 1168, 1371
Hsp92II CATG 7 cut(s) 266, 283, 569, 629, 664, 1079, 1114
HspAI GCGC 1 cut(s) 1015
Kzo9I GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
LmnI GCTCC 2 cut(s) 166, 1357
Lsp1109I GCAGC 3 cut(s) 457, 1195, 1347
LweI GCATC 4 cut(s) 45, 172, 265, 1032
MaeI CTAG 4 cut(s) 59, 585, 735, 1299
MaeII ACGT 3 cut(s) 649, 1168, 1371
MaeIII GTNAC 3 cut(s) 1052, 1100, 1294
MalI GATC 9 cut(s) 215, 600, 750, 755, 836, 963, 1126, 1158, 1380
MboI GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
MboII GAAGA 7 cut(s) 80, 232, 602, 671, 947, 1281, 1373
MflI RGATCY 2 cut(s) 213, 753
MhlI GDGCHC 3 cut(s) 175, 538, 891
MluCI AATT 8 cut(s) 357, 438, 606, 911, 1118, 1200, 1204, 1288
MlyI GAGTC 1 cut(s) 1213
MseI TTAA 6 cut(s) 302, 696, 876, 921, 1005, 1203
MslI CAYNNNNRTG 2 cut(s) 698, 1109
MspR9I CCNGG 3 cut(s) 476, 499, 645
Mva1269I GAATGC 1 cut(s) 1320
MvaI CCWGG 3 cut(s) 476, 499, 645
MwoI GCNNNNNNNGC 3 cut(s) 158, 886, 1192
NcoI CCATGG 2 cut(s) 279, 1075
NdeII GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
NlaIII CATG 7 cut(s) 266, 283, 569, 629, 664, 1079, 1114
NlaIV GGNNCC 2 cut(s) 492, 1132
NmeAIII GCCGAG 1 cut(s) 531
NmuCI GTSAC 3 cut(s) 1052, 1100, 1294
NspI RCATGY 2 cut(s) 629, 664
PagI TCATGA 1 cut(s) 262
PciI ACATGT 1 cut(s) 660
PcsI WCGNNNNNNNCGW 2 cut(s) 1249, 1258
PctI GAATGC 1 cut(s) 1320
PfeI GAWTC 4 cut(s) 197, 343, 388, 903
PflMI CCANNNNNTGG 1 cut(s) 481
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 3 cut(s) 447, 1185, 1362
PleI GAGTC 1 cut(s) 1213
PmaCI CACGTG 1 cut(s) 1169
PmlI CACGTG 1 cut(s) 1169
PpsI GAGTC 1 cut(s) 1213
Ppu21I YACGTR 1 cut(s) 1169
PscI ACATGT 1 cut(s) 660
PshBI ATTAAT 1 cut(s) 1203
PsiI TTATAA 1 cut(s) 1341
Psp124BI GAGCTC 1 cut(s) 891
Psp6I CCWGG 3 cut(s) 474, 497, 643
PspCI CACGTG 1 cut(s) 1169
PspGI CCWGG 3 cut(s) 474, 497, 643
PspN4I GGNNCC 2 cut(s) 492, 1132
PspPI GGNCC 2 cut(s) 466, 1087
PstNI CAGNNNCTG 1 cut(s) 775
PsuI RGATCY 2 cut(s) 213, 753
RseI CAYNNNNRTG 2 cut(s) 698, 1109
SacI GAGCTC 1 cut(s) 891
SaqAI TTAA 6 cut(s) 302, 696, 876, 921, 1005, 1203
SatI GCNGC 3 cut(s) 446, 1184, 1361
Sau3AI GATC 9 cut(s) 213, 598, 748, 753, 834, 961, 1124, 1156, 1378
Sau96I GGNCC 2 cut(s) 466, 1087
SchI GAGTC 1 cut(s) 1213
ScrFI CCNGG 3 cut(s) 476, 499, 645
SduI GDGCHC 3 cut(s) 175, 538, 891
SfaNI GCATC 4 cut(s) 45, 172, 265, 1032
SinI GGWCC 2 cut(s) 466, 1087
SmiMI CAYNNNNRTG 2 cut(s) 698, 1109
SmlI CTYRAG 3 cut(s) 830, 896, 947
SmoI CTYRAG 3 cut(s) 830, 896, 947
SpeI ACTAGT 1 cut(s) 584
Sse9I AATT 8 cut(s) 357, 438, 606, 911, 1118, 1200, 1204, 1288
SsiI CCGC 2 cut(s) 532, 667
SspMI CTAG 4 cut(s) 59, 585, 735, 1299
SstI GAGCTC 1 cut(s) 891
StyD4I CCNGG 3 cut(s) 474, 497, 643
StyI CCWWGG 2 cut(s) 279, 1075
TaaI ACNGT 4 cut(s) 177, 192, 269, 1063
TaiI ACGT 3 cut(s) 652, 1171, 1374
TaqI TCGA 1 cut(s) 927
TasI AATT 8 cut(s) 357, 438, 606, 911, 1118, 1200, 1204, 1288
TfiI GAWTC 4 cut(s) 197, 343, 388, 903
Tru1I TTAA 6 cut(s) 302, 696, 876, 921, 1005, 1203
Tru9I TTAA 6 cut(s) 302, 696, 876, 921, 1005, 1203
TscAI CASTG 2 cut(s) 476, 1066
TseFI GTSAC 3 cut(s) 1052, 1100, 1294
TseI GCWGC 3 cut(s) 445, 1183, 1360
Tsp45I GTSAC 3 cut(s) 1052, 1100, 1294
TspDTI ATGAA 8 cut(s) 120, 221, 251, 554, 729, 738, 866, 1229
TspGWI ACGGA 1 cut(s) 108
TspRI CASTG 2 cut(s) 476, 1066
Van91I CCANNNNNTGG 1 cut(s) 481
VneI GTGCAC 1 cut(s) 171
VpaK11BI GGWCC 2 cut(s) 466, 1087
VspI ATTAAT 1 cut(s) 1203
XapI RAATTY 3 cut(s) 438, 606, 1118
XceI RCATGY 2 cut(s) 629, 664
XspI CTAG 4 cut(s) 59, 585, 735, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.