Rh6CG517800

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000799
Physical Location & Seq
Reverse (-)
67459195 .. 67462400
3206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG517800.1

Sequence Viewer

Length: 1242 bp
ATGGCTGGCGCTGCACCGGAAGGATCTCAATTTGACGCTCGTCAATACGATGCGAAGATGAGCGAGCTACTTGGATCTGATGGACAAGACTTCTTCACCTCGTATGATGAGGTTCATGACAGTTTTGATGTCATGGGACTGCAGGAGAACCTTCTCAGGGGCATCTATGCATATGGTTTTGAGAAGCCCTCTGCAATTCAGCAAAGGGGAATTGTTCCCTTCTGCAAGGGACTTGATGTGATTCAACAGGCACAATCTGGAACTGGAAAAACAGCCACTTTCTGCTCTGGAATTTTACAGCAGCTTGATTATGGCCTACTCGATTGTCAGGCACTAGTTCTTGCACCTACCCGTGAACTTGCTCAACAGATTGAGAAGGTTATGCGGGCTCTGGGTGACTATCTCGGTGTCAAAGTTCATGCTTGTGTTGGCGGGACCAGTGTTCGTGAAGATCAACGTGTCCTCTCTAGTGGAGTCCATGTTGTTGTTGGTACACCTGGTCGAGTATTTGACATGTTGCGGAGACAGTCTCTCCGTCCAGATTGCATTAGGATGTTTGTGTTAGATGAAGCTGATGAAATGCTCTCACGAGGTTTCAAGGATCAGATCTATGACATTTTCCAGCTGCTACCACCCAAAATTCAGGTGGGAGTTTTTTCTGCCACAATGCCACCAGAAGCCCTTGATATCACCAGGAAGTTCATGAACAAACCTGTGAGGATTTTGGTGAAACGTGATGAGCTCACTCTTGAGGGTATCAAGCAGTTCCATGTCAATGTTGACAAGGAGGAGTGGAAACTCGAGACGCTTTGCGATCTTTATGAGACCTTGGCAATCACCCAGAGTGTCATCTTTGTGAACACCAGGCGCAAAGTTGATTGGCTGACAGACAAGATGCGCAGCCGTGATCACACGGTCTCCGCCACCCATGGTGACATGGAGCAAAACACTAGGGACATCATCATGAGAGAGTTTAGGTCTGGTTCTTCTCGTGTGCTCATAACAACTGATCTGTTGGCCCGTGGTATTGATGTCCAGCAGGTCTCTCTTGTGATTAACTATGACCTTCCAACACAGCCGGAAAACTACCTCCATCGTATTGGTCGTAGTGGTAGGTTTGGGAGGAAGGGTGTTGCCATCAACTTTGTGACCAAGGATGATGAAAGGATGTTGTTCGACATCCAGAGGTTCTACAATGTTGTAATTGAGGAGCTGCCGGCTAATGTTGCTGATCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

413

Amino Acids

46.69

Weight (kDa)

5.53

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 65 - 227 2.3e-42 DEAD/DEAH box helicase
ResIII PF04851 83 - 223 6.5e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 266 - 374 6.8e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 1030
AciI CCGC 4 cut(s) 385, 432, 520, 921
AclWI GGATC 3 cut(s) 31, 82, 609
AcsI RAATTY 2 cut(s) 291, 639
AfaI GTAC 1 cut(s) 493
AfiI CCNNNNNNNGG 1 cut(s) 157
AflIII ACRYGT 2 cut(s) 457, 513
AgsI TTSAA 2 cut(s) 245, 598
AhlI ACTAGT 1 cut(s) 334
AjnI CCWGG 3 cut(s) 496, 692, 863
AluBI AGCT 6 cut(s) 67, 304, 572, 625, 742, 1213
AluI AGCT 6 cut(s) 67, 304, 572, 625, 742, 1213
Alw21I GWGCWC 2 cut(s) 744, 999
Alw26I GTCTC 6 cut(s) 517, 534, 797, 818, 922, 1048
AlwI GGATC 3 cut(s) 31, 82, 609
Ama87I CYCGRG 1 cut(s) 800
AoxI GGCC 2 cut(s) 313, 1017
ApeKI GCWGC 5 cut(s) 11, 301, 625, 900, 1213
ApoI RAATTY 2 cut(s) 291, 639
AspLEI GCGC 3 cut(s) 11, 870, 900
AspS9I GGNCC 2 cut(s) 435, 1018
AsuHPI GGTGA 6 cut(s) 88, 407, 682, 739, 829, 944
AvaI CYCGRG 1 cut(s) 800
AvaII GGWCC 1 cut(s) 435
BanII GRGCYC 2 cut(s) 391, 744
BauI CACGAG 2 cut(s) 588, 990
Bbv12I GWGCWC 2 cut(s) 744, 999
BbvI GCAGC 4 cut(s) 313, 612, 912, 1200
BccI CCATC 3 cut(s) 74, 1101, 1145
BceAI ACGGC 1 cut(s) 888
BciT130I CCWGG 3 cut(s) 498, 694, 865
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 6 cut(s) 517, 534, 797, 818, 922, 1048
BcuI ACTAGT 1 cut(s) 334
BfaI CTAG 3 cut(s) 335, 468, 951
BfmI CTRYAG 1 cut(s) 140
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 1030
BglII AGATCT 1 cut(s) 606
BisI GCNGC 5 cut(s) 12, 302, 626, 901, 1214
BlsI GCNGC 5 cut(s) 13, 303, 627, 902, 1215
Bme1390I CCNGG 3 cut(s) 498, 694, 865
Bme18I GGWCC 1 cut(s) 435
BmeT110I CYCGRG 1 cut(s) 800
BmgT120I GGNCC 2 cut(s) 435, 1018
BmiI GGNNCC 1 cut(s) 436
BmrFI CCNGG 3 cut(s) 498, 694, 865
BmsI GCATC 3 cut(s) 40, 171, 885
BoxI GACNNNNGTC 1 cut(s) 39
BplI GAGNNNNNCTC 4 cut(s) 173, 205, 514, 546
BpuEI CTTGAG 1 cut(s) 770
BsaI GGTCTC 3 cut(s) 818, 922, 1048
BsaJI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 8 cut(s) 465, 495, 516, 546, 902, 932, 1114, 1144
Bsc4I CCNNNNNNNGG 1 cut(s) 157
Bse118I RCCGGY 1 cut(s) 1216
Bse1I ACTGG 2 cut(s) 268, 438
BseBI CCWGG 3 cut(s) 498, 694, 865
BseDI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BseGI GGATG 4 cut(s) 558, 1162, 1173, 1179
BseLI CCNNNNNNNGG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 169
BseNI ACTGG 2 cut(s) 268, 438
BseRI GAGGAG 2 cut(s) 803, 1223
BseXI GCAGC 4 cut(s) 313, 612, 912, 1200
BshFI GGCC 2 cut(s) 315, 1019
BsiHKAI GWGCWC 2 cut(s) 744, 999
BsiHKCI CYCGRG 1 cut(s) 800
BsiSI CCGG 3 cut(s) 17, 1079, 1217
BslFI GGGAC 4 cut(s) 150, 243, 448, 968
BslI CCNNNNNNNGG 1 cut(s) 157
BsmAI GTCTC 6 cut(s) 517, 534, 797, 818, 922, 1048
BsmBI CGTCTC 1 cut(s) 797
BsmFI GGGAC 4 cut(s) 150, 243, 448, 968
BsnI GGCC 2 cut(s) 315, 1019
Bso31I GGTCTC 3 cut(s) 818, 922, 1048
BsoBI CYCGRG 1 cut(s) 800
Bsp1286I GDGCHC 3 cut(s) 391, 744, 999
Bsp143I GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
Bsp19I CCATGG 1 cut(s) 928
BspACI CCGC 4 cut(s) 385, 432, 520, 921
BspANI GGCC 2 cut(s) 315, 1019
BspCNI CTCAG 1 cut(s) 168
BspHI TCATGA 3 cut(s) 115, 702, 963
BspLI GGNNCC 1 cut(s) 436
BspMAI CTGCAG 1 cut(s) 144
BspMI ACCTGC 1 cut(s) 1030
BspPI GGATC 3 cut(s) 31, 82, 609
BspTNI GGTCTC 3 cut(s) 818, 922, 1048
BsrFI RCCGGY 1 cut(s) 1216
BsrI ACTGG 2 cut(s) 268, 438
BssAI RCCGGY 1 cut(s) 1216
BssECI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BssMI GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
BssSI CACGAG 2 cut(s) 588, 990
BssT1I CCWWGG 3 cut(s) 828, 928, 1152
Bst2BI CACGAG 2 cut(s) 588, 990
Bst2UI CCWGG 3 cut(s) 498, 694, 865
Bst4CI ACNGT 3 cut(s) 122, 528, 916
BstC8I GCNNGC 4 cut(s) 7, 65, 387, 1218
BstDEI CTNAG 1 cut(s) 155
BstDSI CCRYGG 2 cut(s) 928, 1021
BstF5I GGATG 4 cut(s) 558, 1162, 1173, 1179
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 3 cut(s) 11, 870, 900
BstKTI GATC 9 cut(s) 26, 77, 454, 604, 609, 817, 910, 1012, 1234
BstMAI GTCTC 6 cut(s) 517, 534, 797, 818, 922, 1048
BstMBI GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
BstMWI GCNNNNNNNGC 2 cut(s) 11, 1226
BstNI CCWGG 3 cut(s) 498, 694, 865
BstNSI RCATGY 1 cut(s) 517
BstPAI GACNNNNGTC 1 cut(s) 39
BstSCI CCNGG 3 cut(s) 496, 692, 863
BstSFI CTRYAG 1 cut(s) 140
BstV1I GCAGC 4 cut(s) 313, 612, 912, 1200
BstX2I RGATCY 3 cut(s) 23, 74, 606
BstXI CCANNNNNNTGG 1 cut(s) 1100
BstYI RGATCY 3 cut(s) 23, 74, 606
BsuRI GGCC 2 cut(s) 315, 1019
BtgI CCRYGG 2 cut(s) 928, 1021
BtsCI GGATG 4 cut(s) 558, 1162, 1173, 1179
BtsIMutI CAGTG 1 cut(s) 445
BveI ACCTGC 1 cut(s) 1030
Cac8I GCNNGC 4 cut(s) 7, 65, 387, 1218
CciI TCATGA 3 cut(s) 115, 702, 963
CfoI GCGC 3 cut(s) 11, 870, 900
Cfr10I RCCGGY 1 cut(s) 1216
Cfr13I GGNCC 2 cut(s) 435, 1018
CseI GACGC 2 cut(s) 44, 814
CsiI ACCWGGT 1 cut(s) 496
Csp6I GTAC 1 cut(s) 492
CviQI GTAC 1 cut(s) 492
DdeI CTNAG 1 cut(s) 155
DpnI GATC 9 cut(s) 25, 76, 453, 603, 608, 816, 909, 1011, 1233
DpnII GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
EciI GGCGGA 1 cut(s) 910
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 3 cut(s) 828, 928, 1152
Eco24I GRGCYC 2 cut(s) 391, 744
Eco31I GGTCTC 3 cut(s) 818, 922, 1048
Eco32I GATATC 1 cut(s) 688
Eco47I GGWCC 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 742
Eco88I CYCGRG 1 cut(s) 800
EcoICRI GAGCTC 1 cut(s) 742
EcoRII CCWGG 3 cut(s) 496, 692, 863
EcoRV GATATC 1 cut(s) 688
EcoT14I CCWWGG 3 cut(s) 828, 928, 1152
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 2 cut(s) 391, 744
ErhI CCWWGG 3 cut(s) 828, 928, 1152
Esp3I CGTCTC 1 cut(s) 797
FaqI GGGAC 4 cut(s) 150, 243, 448, 968
FauI CCCGC 2 cut(s) 378, 425
FauNDI CATATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 5 cut(s) 12, 302, 626, 901, 1214
FokI GGATG 4 cut(s) 565, 1166, 1169, 1180
FriOI GRGCYC 2 cut(s) 391, 744
Fsp4HI GCNGC 5 cut(s) 12, 302, 626, 901, 1214
FspBI CTAG 3 cut(s) 335, 468, 951
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 3 cut(s) 10, 869, 899
GluI GCNGC 5 cut(s) 12, 302, 626, 901, 1214
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 315, 1019
HapII CCGG 3 cut(s) 17, 1079, 1217
HgaI GACGC 2 cut(s) 44, 814
HhaI GCGC 3 cut(s) 11, 870, 900
Hin6I GCGC 3 cut(s) 9, 868, 898
HinP1I GCGC 3 cut(s) 9, 868, 898
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HinfI GANTC 2 cut(s) 241, 474
HpaII CCGG 3 cut(s) 17, 1079, 1217
HphI GGTGA 6 cut(s) 88, 407, 682, 739, 829, 944
Hpy166II GTNNAC 4 cut(s) 356, 494, 781, 859
Hpy188I TCNGA 2 cut(s) 79, 606
Hpy8I GTNNAC 4 cut(s) 356, 494, 781, 859
HpyAV CCTTC 6 cut(s) 14, 161, 229, 370, 1076, 1120
HpyCH4III ACNGT 3 cut(s) 122, 528, 916
HpyCH4IV ACGT 2 cut(s) 457, 733
HpyCH4V TGCA 7 cut(s) 14, 142, 170, 194, 225, 344, 546
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 1226
HpyF3I CTNAG 1 cut(s) 155
HpySE526I ACGT 2 cut(s) 457, 733
HspAI GCGC 3 cut(s) 9, 868, 898
KroI GCCGGC 1 cut(s) 1216
KroNI GCCGGC 1 cut(s) 1218
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
LmnI GCTCC 2 cut(s) 940, 1210
Lsp1109I GCAGC 4 cut(s) 313, 612, 912, 1200
LweI GCATC 3 cut(s) 40, 171, 885
MabI ACCWGGT 1 cut(s) 496
MaeI CTAG 3 cut(s) 335, 468, 951
MaeII ACGT 2 cut(s) 457, 733
MaeIII GTNAC 3 cut(s) 395, 932, 1147
MalI GATC 9 cut(s) 25, 76, 453, 603, 608, 816, 909, 1011, 1233
MboI GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
MboII GAAGA 5 cut(s) 67, 85, 461, 978, 1226
MflI RGATCY 3 cut(s) 23, 74, 606
MhlI GDGCHC 3 cut(s) 391, 744, 999
MluCI AATT 6 cut(s) 29, 195, 210, 291, 639, 1203
MlyI GAGTC 1 cut(s) 483
MmeI TCCRAC 1 cut(s) 1094
Mph1103I ATGCAT 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 1216
MseI TTAA 2 cut(s) 1056, 1240
MslI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 3 cut(s) 17, 1079, 1217
MspR9I CCNGG 3 cut(s) 498, 694, 865
MvaI CCWGG 3 cut(s) 498, 694, 865
MwoI GCNNNNNNNGC 2 cut(s) 11, 1226
NaeI GCCGGC 1 cut(s) 1218
NcoI CCATGG 1 cut(s) 928
NdeI CATATG 1 cut(s) 172
NdeII GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
NgoMIV GCCGGC 1 cut(s) 1216
NlaIV GGNNCC 1 cut(s) 436
NmuCI GTSAC 3 cut(s) 395, 932, 1147
NsbI TGCGCA 1 cut(s) 899
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 517
PaeR7I CTCGAG 1 cut(s) 800
PagI TCATGA 3 cut(s) 115, 702, 963
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 1 cut(s) 1102
PdiI GCCGGC 1 cut(s) 1218
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 5 cut(s) 13, 303, 627, 902, 1215
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PscI ACATGT 1 cut(s) 513
PshAI GACNNNNGTC 1 cut(s) 39
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 3 cut(s) 496, 692, 863
PspGI CCWGG 3 cut(s) 496, 692, 863
PspN4I GGNNCC 1 cut(s) 436
PspPI GGNCC 2 cut(s) 435, 1018
PstI CTGCAG 1 cut(s) 144
PsuI RGATCY 3 cut(s) 23, 74, 606
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 1 cut(s) 493
RsaNI GTAC 1 cut(s) 492
RseI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 2 cut(s) 1056, 1240
SatI GCNGC 5 cut(s) 12, 302, 626, 901, 1214
Sau3AI GATC 9 cut(s) 23, 74, 451, 601, 606, 814, 907, 1009, 1231
Sau96I GGNCC 2 cut(s) 435, 1018
SchI GAGTC 1 cut(s) 483
ScrFI CCNGG 3 cut(s) 498, 694, 865
SduI GDGCHC 3 cut(s) 391, 744, 999
SexAI ACCWGGT 1 cut(s) 496
SfaNI GCATC 3 cut(s) 40, 171, 885
SfcI CTRYAG 1 cut(s) 140
Sfr274I CTCGAG 1 cut(s) 800
SinI GGWCC 1 cut(s) 435
SlaI CTCGAG 1 cut(s) 800
SmiMI CAYNNNNRTG 5 cut(s) 423, 551, 774, 854, 962
SmlI CTYRAG 2 cut(s) 749, 800
SmoI CTYRAG 2 cut(s) 749, 800
SpeI ACTAGT 1 cut(s) 334
Sse9I AATT 6 cut(s) 29, 195, 210, 291, 639, 1203
SsiI CCGC 4 cut(s) 385, 432, 520, 921
SspMI CTAG 3 cut(s) 335, 468, 951
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 496, 692, 863
StyI CCWWGG 3 cut(s) 828, 928, 1152
TaaI ACNGT 3 cut(s) 122, 528, 916
TaiI ACGT 2 cut(s) 460, 736
TaqI TCGA 4 cut(s) 321, 502, 801, 1176
TasI AATT 6 cut(s) 29, 195, 210, 291, 639, 1203
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 2 cut(s) 1056, 1240
Tru9I TTAA 2 cut(s) 1056, 1240
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 3 cut(s) 395, 932, 1147
TseI GCWGC 5 cut(s) 11, 301, 625, 900, 1213
Tsp45I GTSAC 3 cut(s) 395, 932, 1147
TspDTI ATGAA 7 cut(s) 104, 407, 582, 591, 691, 719, 1176
TspGWI ACGGA 1 cut(s) 524
TspRI CASTG 1 cut(s) 445
VpaK11BI GGWCC 1 cut(s) 435
XapI RAATTY 2 cut(s) 291, 639
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 2 cut(s) 485, 643
XhoI CTCGAG 1 cut(s) 800
XspI CTAG 3 cut(s) 335, 468, 951
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.