MD08G1065300.v1.1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000799
Physical Location & Seq
Reverse (-)
5204280 .. 5206292
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1065300.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGCTGCTGTGGCACCGGAAGGATCCCAATTTGATACTCATCAGTATGATGCAAAAATGAGTGAGTTACTTGGAGCTGATGGGCAAGACTTCTTCACATCGTATGACGAGATTCATGACAGCTTTGACGCTATGGGATTGCAGGAGAACCTTCTTCGGGGTATCTATGCATATGGTTTTGAGAAGCCCTCTGCAATTCAACAAAGGGGAATTGTTCCTTTCTGCAAGGGACTTGATGTGATTCAGCAGGCACAATCTGGAACTGGAAAAACAGCGACTTTCTGCTCTGGGATTCTGCAGCAGCTTGATTATGGCTTACTTGATTGCCAAGCATTGGTTCTTGCACCCACACGAGAGCTTGCTCAACAGATCGAAAAGGTTATGAGAGCACTGGGTGACTATCTTGGTGTCAAGGTCCATGCTTGTGTTGGTGGAACCAGTGTTCGTGAAGATCAACGCATTCTCTCCAGTGGAGTCCATGTCGTTGTTGGCACTCCTGGTCGTGTTTTTGACATGCTACGAAGGCAGTCTCTTCGTTCTGATTACATTAAGATGTTCGTGTTAGATGAAGCAGATGAAATGCTTTCACGAGGTTTCAAGGATCAGATCTATGACATTTTCCAGCTGCTACCACCTAAAATCCAGGTGGGGGTTTTCTCTGCCACAATGCCACCAGAGGCCCTTGATATCACTAGGAAGTTCTTGAACAAACCTGTAAGGATTTTGGTGAAACGTGACGAGCTCACCCTTGAAGGTATCAAGCAGTTTCATGTCAATGTTGAGAAAGACGATTGGAAACTTGAGACACTTTGTGATCTTTATGAGACCTTGGCAATTACACAGAGTGTCATCTTTGTGAACACCAGGCGCAAAGTTGAATGGTTAACAGACAAGATGCGCAGCCGTGATCACACAGTTTCTGCCACCCACGGAGACATGGACCAAAACACAAGGGATATCATCATGAGAGAGTTTCGGTCTGGTTCTTCTCGTGTGCTCATAACCACTGATCTGTTGGCCCGTGGTATTGACGTCCAACAGGTCTCTCTTGTGATTAATTACGACCTTCCAACACAACCAGAGAACTACCTCCATCGTATTGGTCGTAGTGGACGGTATGGGAGGAAGGGTGTTGCCATCAATTTCGTCACCAAGGATGACGACAGGATGTTGTTTGATATCCAGAAGTTTTATAACGTTGTCATTGAGGAGCTGCCGGCAAATGTTGCTGATCTGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

414

Amino Acids

46.69

Weight (kDa)

5.49

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 65 - 227 4.1e-43 DEAD/DEAH box helicase
ResIII PF04851 83 - 223 1e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 266 - 374 1e-31 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1194
AatII GACGTC 1 cut(s) 1035
Acc16I TGCGCA 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 13
AccB7I CCANNNNNTGG 1 cut(s) 334
AclI AACGTT 1 cut(s) 1197
AclWI GGATC 3 cut(s) 18, 31, 609
AcyI GRCGYC 1 cut(s) 1032
AdeI CACNNNGTG 3 cut(s) 395, 812, 845
AfiI CCNNNNNNNGG 3 cut(s) 157, 334, 649
AgsI TTSAA 5 cut(s) 200, 598, 706, 752, 878
AjnI CCWGG 3 cut(s) 496, 642, 863
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AloI GAACNNNNNNTCC 2 cut(s) 426, 458
AluBI AGCT 7 cut(s) 77, 123, 304, 358, 625, 742, 1213
AluI AGCT 7 cut(s) 77, 123, 304, 358, 625, 742, 1213
Alw21I GWGCWC 3 cut(s) 391, 744, 999
Alw26I GTCTC 5 cut(s) 534, 797, 818, 927, 1048
AlwI GGATC 3 cut(s) 18, 31, 609
AlwNI CAGNNNCTG 1 cut(s) 920
AoxI GGCC 2 cut(s) 678, 1017
ApeKI GCWGC 6 cut(s) 5, 298, 301, 625, 900, 1213
AseI ATTAAT 1 cut(s) 1056
AspLEI GCGC 2 cut(s) 870, 900
AspS9I GGNCC 4 cut(s) 415, 679, 940, 1018
AsuHPI GGTGA 4 cut(s) 407, 736, 739, 1141
AvaII GGWCC 2 cut(s) 415, 940
BamHI GGATCC 1 cut(s) 23
BanI GGYRCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 744
BauI CACGAG 3 cut(s) 351, 588, 990
Bbv12I GWGCWC 3 cut(s) 391, 744, 999
BbvI GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BccI CCATC 3 cut(s) 74, 1101, 1145
BceAI ACGGC 1 cut(s) 888
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 3 cut(s) 498, 644, 865
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 5 cut(s) 534, 797, 818, 927, 1048
BfaI CTAG 1 cut(s) 693
BfmI CTRYAG 1 cut(s) 296
BglII AGATCT 1 cut(s) 606
BisI GCNGC 6 cut(s) 6, 299, 302, 626, 901, 1214
BlsI GCNGC 6 cut(s) 7, 300, 303, 627, 902, 1215
Bme1390I CCNGG 3 cut(s) 498, 644, 865
Bme18I GGWCC 2 cut(s) 415, 940
BmgT120I GGNCC 4 cut(s) 415, 679, 940, 1018
BmiI GGNNCC 3 cut(s) 15, 25, 436
BmrFI CCNGG 3 cut(s) 498, 644, 865
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 2 cut(s) 40, 885
BmuI ACTGGG 1 cut(s) 401
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpmI CTGGAG 1 cut(s) 451
BpuEI CTTGAG 1 cut(s) 821
BsaBI GATNNNNATC 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 1032
BsaI GGTCTC 2 cut(s) 818, 1048
BsaJI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 1114, 1144
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 334, 649
Bse118I RCCGGY 1 cut(s) 1216
Bse1I ACTGG 4 cut(s) 268, 396, 438, 468
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 498, 644, 865
BseDI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BseGI GGATG 2 cut(s) 1162, 1173
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 3 cut(s) 157, 334, 649
BseNI ACTGG 4 cut(s) 268, 396, 438, 468
BseRI GAGGAG 1 cut(s) 1223
BseXI GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BshFI GGCC 2 cut(s) 680, 1019
BshNI GGYRCC 1 cut(s) 13
BsiHKAI GWGCWC 3 cut(s) 391, 744, 999
BsiSI CCGG 2 cut(s) 17, 1217
BslFI GGGAC 1 cut(s) 243
BslI CCNNNNNNNGG 3 cut(s) 157, 334, 649
BsmAI GTCTC 5 cut(s) 534, 797, 818, 927, 1048
BsmFI GGGAC 1 cut(s) 243
BsmI GAATGC 1 cut(s) 459
BsnI GGCC 2 cut(s) 680, 1019
Bso31I GGTCTC 2 cut(s) 818, 1048
Bsp1286I GDGCHC 3 cut(s) 391, 744, 999
Bsp143I GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
BspANI GGCC 2 cut(s) 680, 1019
BspHI TCATGA 2 cut(s) 115, 963
BspLI GGNNCC 3 cut(s) 15, 25, 436
BspMAI CTGCAG 1 cut(s) 300
BspPI GGATC 3 cut(s) 18, 31, 609
BspT107I GGYRCC 1 cut(s) 13
BspTNI GGTCTC 2 cut(s) 818, 1048
BsrFI RCCGGY 1 cut(s) 1216
BsrI ACTGG 4 cut(s) 268, 396, 438, 468
BssAI RCCGGY 1 cut(s) 1216
BssECI CCNNGG 4 cut(s) 828, 928, 1021, 1152
BssMI GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
BssNI GRCGYC 1 cut(s) 1032
BssSI CACGAG 3 cut(s) 351, 588, 990
BssT1I CCWWGG 2 cut(s) 828, 1152
Bst2BI CACGAG 3 cut(s) 351, 588, 990
Bst2UI CCWGG 3 cut(s) 498, 644, 865
Bst4CI ACNGT 2 cut(s) 916, 1116
Bst6I CTCTTC 1 cut(s) 537
BstACI GRCGYC 1 cut(s) 1032
BstAPI GCANNNNNTGC 1 cut(s) 1226
BstC8I GCNNGC 3 cut(s) 249, 360, 1218
BstDSI CCRYGG 2 cut(s) 928, 1021
BstF5I GGATG 2 cut(s) 1162, 1173
BstHHI GCGC 2 cut(s) 870, 900
BstKTI GATC 9 cut(s) 26, 372, 454, 604, 609, 817, 910, 1012, 1234
BstMAI GTCTC 5 cut(s) 534, 797, 818, 927, 1048
BstMBI GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
BstMWI GCNNNNNNNGC 3 cut(s) 11, 523, 1226
BstNI CCWGG 3 cut(s) 498, 644, 865
BstNSI RCATGY 1 cut(s) 517
BstSCI CCNGG 3 cut(s) 496, 642, 863
BstSFI CTRYAG 1 cut(s) 296
BstV1I GCAGC 5 cut(s) 310, 313, 612, 912, 1200
BstX2I RGATCY 2 cut(s) 23, 606
BstXI CCANNNNNNTGG 1 cut(s) 1100
BstYI RGATCY 2 cut(s) 23, 606
BsuRI GGCC 2 cut(s) 680, 1019
BtgI CCRYGG 2 cut(s) 928, 1021
BtsCI GGATG 2 cut(s) 1162, 1173
BtsIMutI CAGTG 4 cut(s) 389, 445, 475, 1005
Cac8I GCNNGC 3 cut(s) 249, 360, 1218
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 2 cut(s) 115, 963
CfoI GCGC 2 cut(s) 870, 900
Cfr10I RCCGGY 1 cut(s) 1216
Cfr13I GGNCC 4 cut(s) 415, 679, 940, 1018
CseI GACGC 1 cut(s) 137
CviAII CATG 7 cut(s) 116, 419, 479, 514, 770, 937, 964
DpnI GATC 9 cut(s) 25, 371, 453, 603, 608, 816, 909, 1011, 1233
DpnII GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
DraIII CACNNNGTG 3 cut(s) 395, 812, 845
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 2 cut(s) 828, 1152
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 2 cut(s) 818, 1048
Eco32I GATATC 3 cut(s) 688, 958, 1180
Eco47I GGWCC 2 cut(s) 415, 940
Eco53kI GAGCTC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 679
EcoRII CCWGG 3 cut(s) 496, 642, 863
EcoRV GATATC 3 cut(s) 688, 958, 1180
EcoT14I CCWWGG 2 cut(s) 828, 1152
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 2 cut(s) 828, 1152
FaeI CATG 7 cut(s) 119, 422, 482, 517, 773, 940, 967
FaqI GGGAC 1 cut(s) 243
FatI CATG 7 cut(s) 115, 418, 478, 513, 769, 936, 963
FauNDI CATATG 1 cut(s) 172
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 6 cut(s) 6, 299, 302, 626, 901, 1214
FokI GGATG 2 cut(s) 1169, 1180
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 6 cut(s) 6, 299, 302, 626, 901, 1214
FspBI CTAG 1 cut(s) 693
FspI TGCGCA 1 cut(s) 899
GlaI GCGC 2 cut(s) 869, 899
GluI GCNGC 6 cut(s) 6, 299, 302, 626, 901, 1214
GsuI CTGGAG 1 cut(s) 451
HaeIII GGCC 2 cut(s) 680, 1019
HapII CCGG 2 cut(s) 17, 1217
HgaI GACGC 1 cut(s) 137
HhaI GCGC 2 cut(s) 870, 900
Hin1I GRCGYC 1 cut(s) 1032
Hin1II CATG 7 cut(s) 119, 422, 482, 517, 773, 940, 967
Hin6I GCGC 2 cut(s) 868, 898
HinP1I GCGC 2 cut(s) 868, 898
HincII GTYRAC 1 cut(s) 885
HindII GTYRAC 1 cut(s) 885
HinfI GANTC 4 cut(s) 112, 241, 292, 474
HpaI GTTAAC 1 cut(s) 885
HpaII CCGG 2 cut(s) 17, 1217
HphI GGTGA 4 cut(s) 407, 736, 739, 1141
Hpy166II GTNNAC 3 cut(s) 859, 885, 1112
Hpy188I TCNGA 2 cut(s) 541, 606
Hpy188III TCNNGA 7 cut(s) 116, 258, 446, 588, 703, 964, 1183
Hpy8I GTNNAC 3 cut(s) 859, 885, 1112
HpyAV CCTTC 6 cut(s) 14, 161, 516, 746, 1076, 1120
HpyCH4III ACNGT 2 cut(s) 916, 1116
HpyCH4IV ACGT 3 cut(s) 733, 1032, 1197
HpyCH4V TGCA 7 cut(s) 53, 142, 170, 194, 225, 298, 344
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 523, 1226
HpySE526I ACGT 3 cut(s) 733, 1032, 1197
Hsp92I GRCGYC 1 cut(s) 1032
Hsp92II CATG 7 cut(s) 119, 422, 482, 517, 773, 940, 967
HspAI GCGC 2 cut(s) 868, 898
KroI GCCGGC 1 cut(s) 1216
KroNI GCCGGC 1 cut(s) 1218
Ksp22I TGATCA 1 cut(s) 907
KspAI GTTAAC 1 cut(s) 885
Kzo9I GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
LmnI GCTCC 2 cut(s) 74, 1210
Lsp1109I GCAGC 5 cut(s) 310, 313, 612, 912, 1200
LweI GCATC 2 cut(s) 40, 885
MaeI CTAG 1 cut(s) 693
MaeII ACGT 3 cut(s) 733, 1032, 1197
MaeIII GTNAC 4 cut(s) 66, 395, 734, 1147
MalI GATC 9 cut(s) 25, 371, 453, 603, 608, 816, 909, 1011, 1233
MboI GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
MboII GAAGA 5 cut(s) 85, 146, 461, 524, 978
MflI RGATCY 2 cut(s) 23, 606
MhlI GDGCHC 3 cut(s) 391, 744, 999
MluCI AATT 6 cut(s) 29, 195, 210, 834, 1057, 1141
MlyI GAGTC 1 cut(s) 483
MmeI TCCRAC 2 cut(s) 1060, 1094
MnlI CCTC 6 cut(s) 199, 584, 670, 1100, 1116, 1201
Mph1103I ATGCAT 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 1216
MseI TTAA 3 cut(s) 549, 884, 1056
MslI CAYNNNNRTG 5 cut(s) 45, 423, 551, 774, 854
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 2 cut(s) 17, 1217
MspR9I CCNGG 3 cut(s) 498, 644, 865
Mva1269I GAATGC 1 cut(s) 459
MvaI CCWGG 3 cut(s) 498, 644, 865
MwoI GCNNNNNNNGC 3 cut(s) 11, 523, 1226
NaeI GCCGGC 1 cut(s) 1218
NdeI CATATG 1 cut(s) 172
NdeII GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
NgoMIV GCCGGC 1 cut(s) 1216
NlaIII CATG 7 cut(s) 119, 422, 482, 517, 773, 940, 967
NlaIV GGNNCC 3 cut(s) 15, 25, 436
NmuCI GTSAC 3 cut(s) 395, 734, 1147
NsbI TGCGCA 1 cut(s) 899
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 517
PagI TCATGA 2 cut(s) 115, 963
PcsI WCGNNNNNNNCGW 1 cut(s) 1102
PctI GAATGC 1 cut(s) 459
PdiI GCCGGC 1 cut(s) 1218
PfeI GAWTC 3 cut(s) 112, 241, 292
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 6 cut(s) 7, 300, 303, 627, 902, 1215
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PshBI ATTAAT 1 cut(s) 1056
PsiI TTATAA 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 744
Psp1406I AACGTT 1 cut(s) 1197
Psp6I CCWGG 3 cut(s) 496, 642, 863
PspGI CCWGG 3 cut(s) 496, 642, 863
PspN4I GGNNCC 3 cut(s) 15, 25, 436
PspPI GGNCC 4 cut(s) 415, 679, 940, 1018
PstI CTGCAG 1 cut(s) 300
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 2 cut(s) 23, 606
PvuII CAGCTG 1 cut(s) 625
RseI CAYNNNNRTG 5 cut(s) 45, 423, 551, 774, 854
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 3 cut(s) 549, 884, 1056
SatI GCNGC 6 cut(s) 6, 299, 302, 626, 901, 1214
Sau3AI GATC 9 cut(s) 23, 369, 451, 601, 606, 814, 907, 1009, 1231
Sau96I GGNCC 4 cut(s) 415, 679, 940, 1018
SchI GAGTC 1 cut(s) 483
ScrFI CCNGG 3 cut(s) 498, 644, 865
SduI GDGCHC 3 cut(s) 391, 744, 999
SfaNI GCATC 2 cut(s) 40, 885
SfcI CTRYAG 1 cut(s) 296
SinI GGWCC 2 cut(s) 415, 940
SmiMI CAYNNNNRTG 5 cut(s) 45, 423, 551, 774, 854
SmlI CTYRAG 1 cut(s) 800
SmoI CTYRAG 1 cut(s) 800
Sse9I AATT 6 cut(s) 29, 195, 210, 834, 1057, 1141
SspMI CTAG 1 cut(s) 693
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 496, 642, 863
StyI CCWWGG 2 cut(s) 828, 1152
TaaI ACNGT 2 cut(s) 916, 1116
TaiI ACGT 3 cut(s) 736, 1035, 1200
TaqI TCGA 1 cut(s) 372
TaqII GACCGA 1 cut(s) 966
TasI AATT 6 cut(s) 29, 195, 210, 834, 1057, 1141
TfiI GAWTC 3 cut(s) 112, 241, 292
Tru1I TTAA 3 cut(s) 549, 884, 1056
Tru9I TTAA 3 cut(s) 549, 884, 1056
TscAI CASTG 4 cut(s) 396, 445, 475, 1012
TseFI GTSAC 3 cut(s) 395, 734, 1147
TseI GCWGC 6 cut(s) 5, 298, 301, 625, 900, 1213
Tsp45I GTSAC 3 cut(s) 395, 734, 1147
TspDTI ATGAA 4 cut(s) 104, 582, 591, 758
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 4 cut(s) 396, 445, 475, 1012
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 2 cut(s) 415, 940
VspI ATTAAT 1 cut(s) 1056
XceI RCATGY 1 cut(s) 517
XcmI CCANNNNNNNNNTGG 3 cut(s) 425, 485, 1012
XspI CTAG 1 cut(s) 693
ZraI GACGTC 1 cut(s) 1033
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.