RLG00000030391

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000799
Physical Location & Seq
Forward (+)
60757379 .. 60765924
8546 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030391

Sequence Viewer

Length: 1293 bp
ATGGCATCAATGTCCTCTCTCTCTCTCTTCTCTGGGATTGGTGGTGCAGAGATAGCTCTTTACCAGCTTGGTATCCTGATGAAGAATGTTGTGTCAGTGGAGATTTCACAGGTGAGCAGAAATGTTGTGAGGTGTTGGTGGGAGCAGACAAACCAGAGAGGAAACTTGTATCACCTTGCTGATGTGCAAGAGTTGAATGCTGACCGCGTAGAGCATTATATCAGTTCCGTTGGCGGGTTTGCACTGGTGATTGGCGGCAGTCCATGCAACAATCTTGCAGGTAGCAATAGACACCATCGGGATGGGCTTAAGGGTAAAGAGTCTTCTCTTTTTTATGACTATTTTCGTATATTGGATTTGCTGAATGATATTAAGGTTGTTAGAGACTTCAAATGCCCTGATGGCACAACAGAGAAGGAGATTGATGCATCAGGAGATGGTGATGTTCTGGGTTTCTTGGATTTGCATCAAGACGAATCGTTGTTTGAGGCAGGACGAAGGTTGCAACCATATTTGACCGAGCTGACAGATGCTGTCAAGGTGTATGACATTTCTTGCCAACTGACTCTGCAGCTTGACCCATACAGTGAGATGACATTATCCACAAGCAAGACAAACAGTGAAGCTAATGTTGCCAGGGCTAGGAAAGCTCTTGAATATTTGCAATTGGTGCCATCTATTCGTACTCCTATGGTACATATGGCAGGTCTTGCTCCGGAAGGTGCACAGTTTGATGCTAAACAGTATGATTCTAAGATGAATGAGATCCTCTCATCTGAAGGCCAAGAGTTCTTCACATCGTATGATGAGGTTCATGACAGTTTTGATGCCATGGGTTTACAAGAAAATCTTTTAAGGGGAATATATGCTTATGGATTTGAGAAGCCTTCTGCGATTCAGCAAAGAGGAATTGTTCCTTTCTGTAAAGGTTTGGATGTCATTCAACAAGCTCAGTCAGGAACAGGCAAAACAGCAACCTTTTGCTCTGGAATTTTGCAGCAACTTGACTATGGTTTGGTCCAGTGCCAGGCTTTGGTGTTGGCACCTACCAGGGAGTTAGCTCAGCAGATTGAGAAGGTTATGCGGGCACTGGGAGACTATCTCGGCGTGAAGGTTCATGCTTGCGTAGGTGGGACTAGTGTTCGTGAGGATCAGCGAATTCTTCAGACTGGTGTGCATGTGGTGGTTGGCACTCCTGGACGTGTGTTTGACATGTTGCGGAGGCAATCTCTTCGTGCAGATTACATTAAGATGTTTGTATTGGATGAAACCTTTTTGCATCAAGTTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

431

Amino Acids

47.71

Weight (kDa)

5.29

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_methylase PF00145 4 - 119 3.6e-07 C-5 cytosine-specific DNA methylase
DEAD PF00270 298 - 423 3.3e-30 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 269, 695
AccB1I GGYRCC 2 cut(s) 670, 1042
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccII CGCG 1 cut(s) 207
AccIII TCCGGA 1 cut(s) 715
AciI CCGC 5 cut(s) 205, 234, 255, 1084, 1219
AclWI GGATC 2 cut(s) 760, 1158
AcsI RAATTY 2 cut(s) 990, 1158
AcuI CTGAAG 2 cut(s) 798, 1148
AfaI GTAC 2 cut(s) 685, 696
AfiI CCNNNNNNNGG 4 cut(s) 234, 642, 1027, 1033
AflII CTTAAG 1 cut(s) 308
AflIII ACRYGT 2 cut(s) 1201, 1212
AgsI TTSAA 4 cut(s) 196, 391, 656, 944
AhlI ACTAGT 1 cut(s) 1136
AjiI CACGTC 1 cut(s) 1202
AjnI CCWGG 4 cut(s) 635, 1026, 1049, 1195
AluBI AGCT 9 cut(s) 56, 67, 523, 574, 626, 650, 950, 1061, 1290
AluI AGCT 9 cut(s) 56, 67, 523, 574, 626, 650, 950, 1061, 1290
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 2 cut(s) 378, 1089
Alw44I GTGCAC 1 cut(s) 723
AlwI GGATC 2 cut(s) 760, 1158
AlwNI CAGNNNCTG 1 cut(s) 533
Aor13HI TCCGGA 1 cut(s) 715
AoxI GGCC 1 cut(s) 781
ApaLI GTGCAC 1 cut(s) 723
ApeKI GCWGC 2 cut(s) 571, 997
ApoI RAATTY 2 cut(s) 990, 1158
AspS9I GGNCC 1 cut(s) 1018
AsuHPI GGTGA 4 cut(s) 124, 164, 259, 452
AvaII GGWCC 1 cut(s) 1018
BaeGI GKGCMC 2 cut(s) 727, 1090
BanI GGYRCC 2 cut(s) 670, 1042
BarI GAAGNNNNNNTAC 2 cut(s) 307, 339
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 2 cut(s) 583, 1009
BccI CCATC 5 cut(s) 296, 303, 395, 431, 682
BcgI CGANNNNNNTGC 2 cut(s) 1214, 1248
BciT130I CCWGG 4 cut(s) 637, 1028, 1051, 1197
BciVI GTATCC 1 cut(s) 83
BcoDI GTCTC 2 cut(s) 378, 1089
BcuI ACTAGT 1 cut(s) 1136
BfaI CTAG 2 cut(s) 642, 1137
BfmI CTRYAG 1 cut(s) 569
BfrI CTTAAG 1 cut(s) 308
BfuAI ACCTGC 2 cut(s) 269, 695
BfuI GTATCC 1 cut(s) 83
BglI GCCNNNNNGGC 1 cut(s) 402
BisI GCNGC 3 cut(s) 256, 572, 998
BlpI GCTNAGC 1 cut(s) 1062
BlsI GCNGC 3 cut(s) 257, 573, 999
Bme1390I CCNGG 4 cut(s) 637, 1028, 1051, 1197
Bme18I GGWCC 1 cut(s) 1018
BmgBI CACGTC 1 cut(s) 1202
BmgT120I GGNCC 1 cut(s) 1018
BmiI GGNNCC 2 cut(s) 672, 1044
BmrFI CCNGG 4 cut(s) 637, 1028, 1051, 1197
BmrI ACTGGG 1 cut(s) 1100
BmsI GCATC 8 cut(s) 14, 415, 437, 475, 520, 724, 817, 1288
BmuI ACTGGG 1 cut(s) 1100
BpiI GAAGAC 1 cut(s) 315
BplI GAGNNNNNCTC 6 cut(s) 755, 787, 1086, 1118, 1213, 1245
Bpu1102I GCTNAGC 1 cut(s) 1062
BsaBI GATNNNNATC 1 cut(s) 465
BsaJI CCNNGG 3 cut(s) 636, 831, 1050
BsaWI WCCGGW 1 cut(s) 715
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 642, 1027, 1033
Bse1I ACTGG 4 cut(s) 249, 1021, 1095, 1174
Bse8I GATNNNNATC 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 715
BseBI CCWGG 4 cut(s) 637, 1028, 1051, 1197
BseDI CCNNGG 3 cut(s) 636, 831, 1050
BseGI GGATG 3 cut(s) 307, 940, 1270
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 4 cut(s) 234, 642, 1027, 1033
BseMII CTCAG 2 cut(s) 965, 1076
BseNI ACTGG 4 cut(s) 249, 1021, 1095, 1174
BseSI GKGCMC 2 cut(s) 727, 1090
BseXI GCAGC 2 cut(s) 583, 1009
BsgI GTGCAG 2 cut(s) 66, 1257
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 783
BshNI GGYRCC 2 cut(s) 670, 1042
BsiHKAI GWGCWC 1 cut(s) 727
BsiSI CCGG 1 cut(s) 716
BslFI GGGAC 1 cut(s) 1147
BslI CCNNNNNNNGG 4 cut(s) 234, 642, 1027, 1033
BsmAI GTCTC 2 cut(s) 378, 1089
BsmFI GGGAC 1 cut(s) 1147
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 1 cut(s) 783
Bsp1286I GDGCHC 2 cut(s) 727, 1090
Bsp13I TCCGGA 1 cut(s) 715
Bsp143I GATC 2 cut(s) 765, 1150
Bsp1720I GCTNAGC 1 cut(s) 1062
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 5 cut(s) 205, 234, 255, 1084, 1219
BspANI GGCC 1 cut(s) 783
BspCNI CTCAG 2 cut(s) 964, 1075
BspEI TCCGGA 1 cut(s) 715
BspFNI CGCG 1 cut(s) 207
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 2 cut(s) 672, 1044
BspMAI CTGCAG 1 cut(s) 573
BspMI ACCTGC 2 cut(s) 269, 695
BspPI GGATC 2 cut(s) 760, 1158
BspT107I GGYRCC 2 cut(s) 670, 1042
BspTI CTTAAG 1 cut(s) 308
BsrI ACTGG 4 cut(s) 249, 1021, 1095, 1174
BssECI CCNNGG 3 cut(s) 636, 831, 1050
BssMI GATC 2 cut(s) 765, 1150
BssT1I CCWWGG 1 cut(s) 831
Bst2UI CCWGG 4 cut(s) 637, 1028, 1051, 1197
Bst4CI ACNGT 5 cut(s) 587, 620, 729, 744, 821
Bst6I CTCTTC 2 cut(s) 32, 1236
BstAFI CTTAAG 1 cut(s) 308
BstAPI GCANNNNNTGC 3 cut(s) 264, 670, 710
BstC8I GCNNGC 2 cut(s) 1086, 1123
BstDEI CTNAG 3 cut(s) 753, 951, 1062
BstDSI CCRYGG 1 cut(s) 831
BstF5I GGATG 3 cut(s) 307, 940, 1270
BstFNI CGCG 1 cut(s) 207
BstKTI GATC 2 cut(s) 768, 1153
BstMAI GTCTC 2 cut(s) 378, 1089
BstMBI GATC 2 cut(s) 765, 1150
BstMWI GCNNNNNNNGC 7 cut(s) 53, 264, 402, 632, 647, 670, 710
BstNI CCWGG 4 cut(s) 637, 1028, 1051, 1197
BstNSI RCATGY 2 cut(s) 1181, 1216
BstSCI CCNGG 4 cut(s) 635, 1026, 1049, 1195
BstSFI CTRYAG 1 cut(s) 569
BstSLI GKGCMC 2 cut(s) 727, 1090
BstUI CGCG 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 583, 1009
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 765
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 1 cut(s) 765
BsuI GTATCC 1 cut(s) 83
BsuRI GGCC 1 cut(s) 783
BtgI CCRYGG 1 cut(s) 831
BtrI CACGTC 1 cut(s) 1202
BtsCI GGATG 3 cut(s) 307, 940, 1270
BtsIMutI CAGTG 6 cut(s) 102, 242, 592, 625, 1028, 1088
BveI ACCTGC 2 cut(s) 269, 695
Cac8I GCNNGC 2 cut(s) 1086, 1123
CaiI CAGNNNCTG 1 cut(s) 533
CciI TCATGA 1 cut(s) 814
Cfr13I GGNCC 1 cut(s) 1018
Csp6I GTAC 2 cut(s) 684, 695
CviAII CATG 6 cut(s) 264, 815, 832, 1118, 1178, 1213
CviQI GTAC 2 cut(s) 684, 695
DdeI CTNAG 3 cut(s) 753, 951, 1062
DpnI GATC 2 cut(s) 767, 1152
DpnII GATC 2 cut(s) 765, 1150
Eam1104I CTCTTC 2 cut(s) 32, 1236
EarI CTCTTC 2 cut(s) 32, 1236
Eco130I CCWWGG 1 cut(s) 831
Eco47I GGWCC 1 cut(s) 1018
Eco57I CTGAAG 2 cut(s) 798, 1148
EcoRI GAATTC 1 cut(s) 1158
EcoRII CCWGG 4 cut(s) 635, 1026, 1049, 1195
EcoT14I CCWWGG 1 cut(s) 831
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 831
FaeI CATG 6 cut(s) 267, 818, 835, 1121, 1181, 1216
FalI AAGNNNNNCTT 2 cut(s) 834, 866
FaqI GGGAC 1 cut(s) 1147
FatI CATG 6 cut(s) 263, 814, 831, 1117, 1177, 1212
FauI CCCGC 2 cut(s) 227, 1077
FauNDI CATATG 1 cut(s) 699
Fnu4HI GCNGC 3 cut(s) 256, 572, 998
FokI GGATG 3 cut(s) 314, 947, 1277
Fsp4HI GCNGC 3 cut(s) 256, 572, 998
FspBI CTAG 2 cut(s) 642, 1137
GluI GCNGC 3 cut(s) 256, 572, 998
HaeIII GGCC 1 cut(s) 783
HapII CCGG 1 cut(s) 716
Hin1II CATG 6 cut(s) 267, 818, 835, 1121, 1181, 1216
HinfI GANTC 5 cut(s) 320, 476, 565, 749, 895
HpaII CCGG 1 cut(s) 716
HphI GGTGA 4 cut(s) 124, 164, 259, 452
Hpy166II GTNNAC 2 cut(s) 725, 839
Hpy188I TCNGA 2 cut(s) 778, 1167
Hpy8I GTNNAC 2 cut(s) 725, 839
HpyAV CCTTC 7 cut(s) 409, 492, 713, 773, 897, 1069, 1105
HpyCH4III ACNGT 5 cut(s) 587, 620, 729, 744, 821
HpyCH4IV ACGT 1 cut(s) 1201
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 264, 402, 632, 647, 670, 710
HpyF3I CTNAG 3 cut(s) 753, 951, 1062
HpySE526I ACGT 1 cut(s) 1201
Hsp92II CATG 6 cut(s) 267, 818, 835, 1121, 1181, 1216
Kpn2I TCCGGA 1 cut(s) 715
Kzo9I GATC 2 cut(s) 765, 1150
LmnI GCTCC 2 cut(s) 142, 718
Lsp1109I GCAGC 2 cut(s) 583, 1009
LweI GCATC 8 cut(s) 14, 415, 437, 475, 520, 724, 817, 1288
MaeI CTAG 2 cut(s) 642, 1137
MaeII ACGT 1 cut(s) 1201
MalI GATC 2 cut(s) 767, 1152
MboI GATC 2 cut(s) 765, 1150
MboII GAAGA 6 cut(s) 19, 94, 315, 784, 1154, 1223
MfeI CAATTG 1 cut(s) 665
MflI RGATCY 1 cut(s) 765
MhlI GDGCHC 2 cut(s) 727, 1090
MluCI AATT 4 cut(s) 665, 909, 990, 1158
MlyI GAGTC 2 cut(s) 329, 559
MnlI CCTC 9 cut(s) 25, 123, 152, 481, 779, 802, 899, 1141, 1215
Mph1103I ATGCAT 1 cut(s) 430
MroI TCCGGA 1 cut(s) 715
MseI TTAA 4 cut(s) 309, 372, 854, 1248
MslI CAYNNNNRTG 2 cut(s) 300, 1250
MspCI CTTAAG 1 cut(s) 308
MspI CCGG 1 cut(s) 716
MspR9I CCNGG 4 cut(s) 637, 1028, 1051, 1197
MunI CAATTG 1 cut(s) 665
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 4 cut(s) 637, 1028, 1051, 1197
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 7 cut(s) 53, 264, 402, 632, 647, 670, 710
NcoI CCATGG 1 cut(s) 831
NdeI CATATG 1 cut(s) 699
NdeII GATC 2 cut(s) 765, 1150
NlaIII CATG 6 cut(s) 267, 818, 835, 1121, 1181, 1216
NlaIV GGNNCC 2 cut(s) 672, 1044
NmeAIII GCCGAG 1 cut(s) 1083
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 2 cut(s) 1181, 1216
PagI TCATGA 1 cut(s) 814
PciI ACATGT 1 cut(s) 1212
PctI GAATGC 1 cut(s) 202
PfeI GAWTC 3 cut(s) 476, 749, 895
PflMI CCANNNNNTGG 1 cut(s) 1033
PfoI TCCNGGA 1 cut(s) 1195
PkrI GCNGC 3 cut(s) 257, 573, 999
PleI GAGTC 2 cut(s) 328, 559
PpsI GAGTC 2 cut(s) 328, 559
PscI ACATGT 1 cut(s) 1212
Psp6I CCWGG 4 cut(s) 635, 1026, 1049, 1195
PspGI CCWGG 4 cut(s) 635, 1026, 1049, 1195
PspN4I GGNNCC 2 cut(s) 672, 1044
PspPI GGNCC 1 cut(s) 1018
PstI CTGCAG 1 cut(s) 573
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 1 cut(s) 765
RsaI GTAC 2 cut(s) 685, 696
RsaNI GTAC 2 cut(s) 684, 695
RseI CAYNNNNRTG 2 cut(s) 300, 1250
SaqAI TTAA 4 cut(s) 309, 372, 854, 1248
SatI GCNGC 3 cut(s) 256, 572, 998
Sau3AI GATC 2 cut(s) 765, 1150
Sau96I GGNCC 1 cut(s) 1018
SchI GAGTC 2 cut(s) 329, 559
ScrFI CCNGG 4 cut(s) 637, 1028, 1051, 1197
SduI GDGCHC 2 cut(s) 727, 1090
SfaNI GCATC 8 cut(s) 14, 415, 437, 475, 520, 724, 817, 1288
SfcI CTRYAG 1 cut(s) 569
SinI GGWCC 1 cut(s) 1018
SmiMI CAYNNNNRTG 2 cut(s) 300, 1250
SmlI CTYRAG 1 cut(s) 308
SmoI CTYRAG 1 cut(s) 308
SpeI ACTAGT 1 cut(s) 1136
Sse9I AATT 4 cut(s) 665, 909, 990, 1158
SsiI CCGC 5 cut(s) 205, 234, 255, 1084, 1219
SspI AATATT 1 cut(s) 659
SspMI CTAG 2 cut(s) 642, 1137
StyD4I CCNGG 4 cut(s) 635, 1026, 1049, 1195
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 5 cut(s) 587, 620, 729, 744, 821
TaiI ACGT 1 cut(s) 1204
TaqII GACCGA 1 cut(s) 533
TasI AATT 4 cut(s) 665, 909, 990, 1158
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 3 cut(s) 476, 749, 895
Tru1I TTAA 4 cut(s) 309, 372, 854, 1248
Tru9I TTAA 4 cut(s) 309, 372, 854, 1248
TscAI CASTG 6 cut(s) 102, 249, 592, 625, 1028, 1095
TseI GCWGC 2 cut(s) 571, 997
TspDTI ATGAA 5 cut(s) 95, 773, 803, 1106, 1281
TspGWI ACGGA 1 cut(s) 217
TspRI CASTG 6 cut(s) 102, 249, 592, 625, 1028, 1095
Van91I CCANNNNNTGG 1 cut(s) 1033
Vha464I CTTAAG 1 cut(s) 308
VneI GTGCAC 1 cut(s) 723
VpaK11BI GGWCC 1 cut(s) 1018
XapI RAATTY 2 cut(s) 990, 1158
XceI RCATGY 2 cut(s) 1181, 1216
XspI CTAG 2 cut(s) 642, 1137
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.