Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0000799

Physical Location & Seq

Genomic Coordinates Reverse (-)

60791038 .. 60792633

Gene Structure 1596 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020078

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

ATGACAGGTGTTGCTAGTGAAAGATCACAATTTGATGCTCATCAATATGATGCTAAGATGAATCAGTTACTTTCAACTGATGGACAAGAATTCTTCACCTCATTCAATGAGGTTTATGAGAGTTTCGACTCTATGGGATTGCAAGAAAATCTCCTTAGAGGCCTTTATGCATATGGTTTTGAGAAGCCTTCTGCGATTCAGCAGAGGGGCATTGTTCCTTTCTGCAGAGGTCTTGATGTGATTCAACAGGCTCAATCTGGAACCGGGAAAACAGCAACTTTCTGCTCTGGAATTTTGCAAAACATTGATTATGATCTTGTCCAATGCCAGGCTTTGGCTTTGGTACCAACCAGAGAGTTGGCACGACAGGTCGAGAAGGTTATGCGAGCACTTGGTGACTATCTTGGCGTGAAGGTTCATGCTTGCGTTGGTGGGACAAGTGTTCGTGAGGATCAACGAATTCTTCAAGCTGGTGTCCATGTTGTTATTGGAACTCCAGGGCGTGTTCTTGACATGTTGAAGAGGCAATGTCTTAGGTCAGATTGCATTAAGATGTTTGTATTGGATGAAGCTGATGAAATGCTTTCTCTTGGTTTCAAGGATCAGATCTATGATATTTTCCAGCAGCTGTCATCGAATATTCAGGTGGGGGTGTTTTCTGCTACTATGCCATCTGAAGCCCTTGAACTCACAAAGAAGTTTATGAACAAACCTGTGAGGATCTTAGTAAAGCGGGATGAGCTCACTCTTGAGGGTATCAAACAATTTTATGTCAATATTGATAAGGAACAATGGAAGTTCGACACACTGTGTGATCTCTACGAGACCTTAACCATCACCCAATGTGTCATCTTTGTCAACACAAGGCGCAATGTGGACTGGCTTACTGACAAGATGCGCAGCCGTGATCACACTGTCTCTGCTATCCATGGTGAAATGGAACAGAACACTCGTGATATCATTATGCGTGAATTCAGATCCGGCTCTTCTCGTGTTCTAATAACCACTGATCTCTTGGCTCGCGGTATTGATGTGCAGCAAGTATCTCTTGTGATAAATTATGATTTGCCAACTCAACCAGAAAATTACCTTCATCGTATAGGACGAAGTGGAAGATTTGGAAGAAAAGGTGTGGCGATCAACTTTGTGACATCAGACAGCGAGAGAATGCTGCATGACATTAAGAACTTTTACCAAGTTGTCATTGAGGAGCTGCCGGACAGATTTGGTGATCTCCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006412 GO:0006413 GO:0006518 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0010468 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019538 GO:0031974 GO:0031981 GO:0034641 GO:0034645 GO:0042623 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0060255 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

DEAD DUF4283 Helicase_C

Protein Analysis

414

Amino Acids

47.01

Weight (kDa)

5.64

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	65 - 227	4.5e-41	DEAD/DEAH box helicase
Helicase_C	PF00271	265 - 374	6.1e-33	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000799)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G54270 AT1G54270 AT1G72730 AT3G13920 AT3G13920 AT3G13920 AT3G13920 AT3G13920
fragaria_vesca	FvH4_1g07920 FvH4_2g31520 FvH4_6g34120 FvH4_6g34120
malus_domestica	MD02G1082700.v1.1 MD08G1065300.v1.1 MD09G1189100.v1.1 MD15G1210800.v1.1 MD17G1172700.v1.1
prunus_persica	Prupe.1G409000_v2.0.a1 Prupe.3G045500_v2.0.a1 Prupe.3G045500_v2.0.a1 Prupe.7G206000_v2.0.a1
pyrus_communis	pycom02g06560 pycom08g05400 pycom09g10600 pycom15g18670 pycom17g17070 pycom17g17080
rosa_chinensis	RchiOBHm_Chr2g0093791 RchiOBHm_Chr2g0144411 RchiOBHm_Chr2g0144421 RchiOBHm_Chr6g0311241
rosa_laevigata	RLG00000010423 RLG00000016437 RLG00000020078 RLG00000020079 RLG00000030391
rosa_multiflora	Rmu_co8454423.1_g000001 Rmu_sc0002126.1_g000004 Rmu_sc0003475.1_g000010 Rmu_sc0012046.1_g000007 Rmu_sc0012116.1_g000001 Rmu_sc0020003.1_g000001
rosa_roxburghii	Rroxscaffold_2G00101330 Rroxscaffold_2G00147800 Rroxscaffold_7G00157830
rosa_rugosa	Rorug02G0040100 Rorug02G0040100 Rorug02G0386200 Rorug02G0386300 Rorug02G0386400 Rorug06G0386800 Rorug06G0386900
rosa_samantha	Rh2AG086300 Rh2AG438600 Rh2AG438700 Rh2BG449000 Rh2CG089000 Rh2CG425700 Rh2CG425800 Rh2DG084700 Rh2DG084800 Rh2DG458700 Rh2DG458800 Rh6BG512000 Rh6CG517800 Rh6DG503900
rosa_wichuraiana	Rw2G035870 Rw2G035880 Rw6G043660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	899
Acc65I	GGTACC	1 cut(s)	343
AccB1I	GGYRCC	1 cut(s)	343
AccB7I	CCANNNNNTGG	1 cut(s)	334
AccII	CGCG	1 cut(s)	1023
AciI	CCGC	2 cut(s)	733, 1023
AclWI	GGATC	4 cut(s)	459, 609, 728, 972
AcsI	RAATTY	4 cut(s)	89, 291, 459, 971
AcuI	CTGAAG	1 cut(s)	696
AdeI	CACNNNGTG	3 cut(s)	395, 810, 812
AfaI	GTAC	1 cut(s)	345
AfiI	CCNNNNNNNGG	2 cut(s)	328, 334
AflIII	ACRYGT	1 cut(s)	513
AgsI	TTSAA	7 cut(s)	75, 106, 245, 467, 520, 598, 686
AjnI	CCWGG	2 cut(s)	327, 496
AluBI	AGCT	5 cut(s)	470, 572, 628, 742, 1213
AluI	AGCT	5 cut(s)	470, 572, 628, 742, 1213
Alw21I	GWGCWC	2 cut(s)	391, 744
Alw26I	GTCTC	2 cut(s)	818, 922
AlwI	GGATC	4 cut(s)	459, 609, 728, 972
AlwNI	CAGNNNCTG	1 cut(s)	628
AoxI	GGCC	1 cut(s)	160
ApeKI	GCWGC	5 cut(s)	625, 900, 1036, 1171, 1213
ApoI	RAATTY	4 cut(s)	89, 291, 459, 971
Asp718I	GGTACC	1 cut(s)	343
AspLEI	GCGC	2 cut(s)	870, 900
AsuC2I	CCSGG	1 cut(s)	265
AsuHPI	GGTGA	5 cut(s)	88, 407, 829, 944, 1241
BanI	GGYRCC	1 cut(s)	343
BanII	GRGCYC	1 cut(s)	744
BauI	CACGAG	2 cut(s)	951, 990
Bbv12I	GWGCWC	2 cut(s)	391, 744
BbvI	GCAGC	5 cut(s)	637, 912, 1048, 1158, 1200
BccI	CCATC	3 cut(s)	74, 679, 842
BceAI	ACGGC	1 cut(s)	888
BciT130I	CCWGG	2 cut(s)	329, 498
BclI	TGATCA	1 cut(s)	907
BcnI	CCSGG	1 cut(s)	265
BcoDI	GTCTC	2 cut(s)	818, 922
BfaI	CTAG	1 cut(s)	15
BfmI	CTRYAG	1 cut(s)	223
BglII	AGATCT	1 cut(s)	606
BisI	GCNGC	5 cut(s)	626, 901, 1037, 1172, 1214
BlsI	GCNGC	5 cut(s)	627, 902, 1038, 1173, 1215
Bme1390I	CCNGG	3 cut(s)	265, 329, 498
BmiI	GGNNCC	2 cut(s)	262, 345
BmrFI	CCNGG	3 cut(s)	265, 329, 498
BmsI	GCATC	3 cut(s)	25, 40, 885
BpmI	CTGGAG	1 cut(s)	480
BpuEI	CTTGAG	1 cut(s)	770
BpuMI	CCSGG	1 cut(s)	265
BsaBI	GATNNNNATC	2 cut(s)	39, 312
BsaI	GGTCTC	1 cut(s)	818
BsaJI	CCNNGG	2 cut(s)	497, 928
Bsc4I	CCNNNNNNNGG	2 cut(s)	328, 334
Bse1I	ACTGG	1 cut(s)	884
Bse3DI	GCAATG	2 cut(s)	533, 877
Bse8I	GATNNNNATC	2 cut(s)	39, 312
BseBI	CCWGG	2 cut(s)	329, 498
BseDI	CCNNGG	2 cut(s)	497, 928
BseGI	GGATG	2 cut(s)	571, 742
BseJI	GATNNNNATC	2 cut(s)	39, 312
BseLI	CCNNNNNNNGG	2 cut(s)	328, 334
BseMI	GCAATG	2 cut(s)	533, 877
BseNI	ACTGG	1 cut(s)	884
BseRI	GAGGAG	2 cut(s)	1223, 1226
BseXI	GCAGC	5 cut(s)	637, 912, 1048, 1158, 1200
BsgI	GTGCAG	1 cut(s)	1055
Bsh1236I	CGCG	1 cut(s)	1023
BshFI	GGCC	1 cut(s)	162
BshNI	GGYRCC	1 cut(s)	343
BsiHKAI	GWGCWC	2 cut(s)	391, 744
BsiSI	CCGG	3 cut(s)	264, 981, 1217
BslFI	GGGAC	1 cut(s)	448
BslI	CCNNNNNNNGG	2 cut(s)	328, 334
BsmAI	GTCTC	2 cut(s)	818, 922
BsmFI	GGGAC	1 cut(s)	448
BsmI	GAATGC	1 cut(s)	1173
BsnI	GGCC	1 cut(s)	162
Bso31I	GGTCTC	1 cut(s)	818
Bsp1286I	GDGCHC	2 cut(s)	391, 744
Bsp19I	CCATGG	1 cut(s)	928
BspACI	CCGC	2 cut(s)	733, 1023
BspANI	GGCC	1 cut(s)	162
BspFNI	CGCG	1 cut(s)	1023
BspLI	GGNNCC	2 cut(s)	262, 345
BspMAI	CTGCAG	1 cut(s)	227
BspPI	GGATC	4 cut(s)	459, 609, 728, 972
BspQI	GCTCTTC	1 cut(s)	991
BspT107I	GGYRCC	1 cut(s)	343
BspTNI	GGTCTC	1 cut(s)	818
BsrDI	GCAATG	2 cut(s)	533, 877
BsrI	ACTGG	1 cut(s)	884
BssECI	CCNNGG	2 cut(s)	497, 928
BssSI	CACGAG	2 cut(s)	951, 990
BssT1I	CCWWGG	1 cut(s)	928
Bst2BI	CACGAG	2 cut(s)	951, 990
Bst2UI	CCWGG	2 cut(s)	329, 498
Bst4CI	ACNGT	2 cut(s)	810, 916
Bst6I	CTCTTC	2 cut(s)	515, 991
BstC8I	GCNNGC	3 cut(s)	387, 424, 1021
BstDEI	CTNAG	4 cut(s)	54, 155, 533, 724
BstDSI	CCRYGG	1 cut(s)	928
BstF5I	GGATG	2 cut(s)	571, 742
BstFNI	CGCG	1 cut(s)	1023
BstHHI	GCGC	2 cut(s)	870, 900
BstMAI	GTCTC	2 cut(s)	818, 922
BstMWI	GCNNNNNNNGC	1 cut(s)	739
BstNI	CCWGG	2 cut(s)	329, 498
BstNSI	RCATGY	1 cut(s)	517
BstSCI	CCNGG	3 cut(s)	263, 327, 496
BstSFI	CTRYAG	1 cut(s)	223
BstUI	CGCG	1 cut(s)	1023
BstV1I	GCAGC	5 cut(s)	637, 912, 1048, 1158, 1200
BstX2I	RGATCY	3 cut(s)	606, 720, 977
BstXI	CCANNNNNNTGG	1 cut(s)	358
BstYI	RGATCY	3 cut(s)	606, 720, 977
BsuRI	GGCC	1 cut(s)	162
BtgI	CCRYGG	1 cut(s)	928
BtsCI	GGATG	2 cut(s)	571, 742
BtsIMutI	CAGTG	3 cut(s)	806, 912, 1005
Cac8I	GCNNGC	3 cut(s)	387, 424, 1021
CaiI	CAGNNNCTG	1 cut(s)	628
CfoI	GCGC	2 cut(s)	870, 900
Csp6I	GTAC	1 cut(s)	344
CviAII	CATG	5 cut(s)	419, 479, 514, 929, 1175
CviQI	GTAC	1 cut(s)	344
DdeI	CTNAG	4 cut(s)	54, 155, 533, 724
DraIII	CACNNNGTG	3 cut(s)	395, 810, 812
Eam1104I	CTCTTC	2 cut(s)	515, 991
EarI	CTCTTC	2 cut(s)	515, 991
Ecl136II	GAGCTC	1 cut(s)	742
Eco130I	CCWWGG	1 cut(s)	928
Eco147I	AGGCCT	1 cut(s)	162
Eco24I	GRGCYC	1 cut(s)	744
Eco31I	GGTCTC	1 cut(s)	818
Eco32I	GATATC	1 cut(s)	958
Eco53kI	GAGCTC	1 cut(s)	742
Eco57I	CTGAAG	1 cut(s)	696
EcoICRI	GAGCTC	1 cut(s)	742
EcoRI	GAATTC	3 cut(s)	89, 459, 971
EcoRII	CCWGG	2 cut(s)	327, 496
EcoRV	GATATC	1 cut(s)	958
EcoT14I	CCWWGG	1 cut(s)	928
EcoT22I	ATGCAT	1 cut(s)	172
EcoT38I	GRGCYC	1 cut(s)	744
ErhI	CCWWGG	1 cut(s)	928
FaeI	CATG	5 cut(s)	422, 482, 517, 932, 1178
FalI	AAGNNNNNCTT	2 cut(s)	1032, 1064
FaqI	GGGAC	1 cut(s)	448
FatI	CATG	5 cut(s)	418, 478, 513, 928, 1174
FauI	CCCGC	1 cut(s)	726
FauNDI	CATATG	1 cut(s)	172
FbaI	TGATCA	1 cut(s)	907
Fnu4HI	GCNGC	5 cut(s)	626, 901, 1037, 1172, 1214
FokI	GGATG	2 cut(s)	578, 749
FriOI	GRGCYC	1 cut(s)	744
Fsp4HI	GCNGC	5 cut(s)	626, 901, 1037, 1172, 1214
FspBI	CTAG	1 cut(s)	15
FspI	TGCGCA	1 cut(s)	899
GlaI	GCGC	2 cut(s)	869, 899
GluI	GCNGC	5 cut(s)	626, 901, 1037, 1172, 1214
GsuI	CTGGAG	1 cut(s)	480
HaeIII	GGCC	1 cut(s)	162
HapII	CCGG	3 cut(s)	264, 981, 1217
HhaI	GCGC	2 cut(s)	870, 900
Hin1II	CATG	5 cut(s)	422, 482, 517, 932, 1178
Hin6I	GCGC	2 cut(s)	868, 898
HinP1I	GCGC	2 cut(s)	868, 898
HincII	GTYRAC	1 cut(s)	859
HindII	GTYRAC	1 cut(s)	859
HinfI	GANTC	4 cut(s)	61, 128, 196, 241
HpaII	CCGG	3 cut(s)	264, 981, 1217
HphI	GGTGA	5 cut(s)	88, 407, 829, 944, 1241
Hpy166II	GTNNAC	2 cut(s)	859, 877
Hpy188I	TCNGA	6 cut(s)	541, 606, 676, 977, 1156, 1241
Hpy188III	TCNNGA	8 cut(s)	233, 258, 288, 373, 446, 509, 749, 953
Hpy8I	GTNNAC	2 cut(s)	859, 877
HpyAV	CCTTC	4 cut(s)	198, 370, 406, 1100
HpyCH4III	ACNGT	2 cut(s)	810, 916
HpyCH4V	TGCA	7 cut(s)	142, 170, 225, 298, 546, 1036, 1174
HpyF10VI	GCNNNNNNNGC	1 cut(s)	739
HpyF3I	CTNAG	4 cut(s)	54, 155, 533, 724
Hsp92II	CATG	5 cut(s)	422, 482, 517, 932, 1178
HspAI	GCGC	2 cut(s)	868, 898
KpnI	GGTACC	1 cut(s)	347
Ksp22I	TGATCA	1 cut(s)	907
LguI	GCTCTTC	1 cut(s)	991
LmnI	GCTCC	1 cut(s)	1210
Lsp1109I	GCAGC	5 cut(s)	637, 912, 1048, 1158, 1200
LweI	GCATC	3 cut(s)	25, 40, 885
MaeI	CTAG	1 cut(s)	15
MaeIII	GTNAC	3 cut(s)	66, 395, 1147
MboII	GAAGA	6 cut(s)	85, 455, 532, 978, 1125, 1134
MflI	RGATCY	3 cut(s)	606, 720, 977
MhlI	GDGCHC	2 cut(s)	391, 744
MluCI	AATT	8 cut(s)	29, 89, 291, 459, 764, 971, 1057, 1084
MlyI	GAGTC	1 cut(s)	122
Mph1103I	ATGCAT	1 cut(s)	172
MseI	TTAA	3 cut(s)	549, 830, 1182
MslI	CAYNNNNRTG	2 cut(s)	45, 551
MspA1I	CMGCKG	1 cut(s)	628
MspI	CCGG	3 cut(s)	264, 981, 1217
MspR9I	CCNGG	3 cut(s)	265, 329, 498
Mva1269I	GAATGC	1 cut(s)	1173
MvaI	CCWGG	2 cut(s)	329, 498
MvnI	CGCG	1 cut(s)	1023
MwoI	GCNNNNNNNGC	1 cut(s)	739
NciI	CCSGG	1 cut(s)	265
NcoI	CCATGG	1 cut(s)	928
NdeI	CATATG	1 cut(s)	172
NlaIII	CATG	5 cut(s)	422, 482, 517, 932, 1178
NlaIV	GGNNCC	2 cut(s)	262, 345
NmuCI	GTSAC	2 cut(s)	395, 1147
NsbI	TGCGCA	1 cut(s)	899
NsiI	ATGCAT	1 cut(s)	172
NspI	RCATGY	1 cut(s)	517
PceI	AGGCCT	1 cut(s)	162
PciI	ACATGT	1 cut(s)	513
PciSI	GCTCTTC	1 cut(s)	991
PcsI	WCGNNNNNNNCGW	1 cut(s)	1102
PctI	GAATGC	1 cut(s)	1173
PfeI	GAWTC	3 cut(s)	61, 196, 241
PflMI	CCANNNNNTGG	1 cut(s)	334
PkrI	GCNGC	5 cut(s)	627, 902, 1038, 1173, 1215
PleI	GAGTC	1 cut(s)	122
PpsI	GAGTC	1 cut(s)	122
PscI	ACATGT	1 cut(s)	513
Psp124BI	GAGCTC	1 cut(s)	744
Psp6I	CCWGG	2 cut(s)	327, 496
PspGI	CCWGG	2 cut(s)	327, 496
PspN4I	GGNNCC	2 cut(s)	262, 345
PstI	CTGCAG	1 cut(s)	227
PstNI	CAGNNNCTG	1 cut(s)	628
PsuI	RGATCY	3 cut(s)	606, 720, 977
PvuII	CAGCTG	1 cut(s)	628
RsaI	GTAC	1 cut(s)	345
RsaNI	GTAC	1 cut(s)	344
RseI	CAYNNNNRTG	2 cut(s)	45, 551
SacI	GAGCTC	1 cut(s)	744
SapI	GCTCTTC	1 cut(s)	991
SaqAI	TTAA	3 cut(s)	549, 830, 1182
SatI	GCNGC	5 cut(s)	626, 901, 1037, 1172, 1214
SchI	GAGTC	1 cut(s)	122
ScrFI	CCNGG	3 cut(s)	265, 329, 498
SduI	GDGCHC	2 cut(s)	391, 744
SfaNI	GCATC	3 cut(s)	25, 40, 885
SfcI	CTRYAG	1 cut(s)	223
SmiMI	CAYNNNNRTG	2 cut(s)	45, 551
SmlI	CTYRAG	1 cut(s)	749
SmoI	CTYRAG	1 cut(s)	749
Sse9I	AATT	8 cut(s)	29, 89, 291, 459, 764, 971, 1057, 1084
SseBI	AGGCCT	1 cut(s)	162
SsiI	CCGC	2 cut(s)	733, 1023
SspI	AATATT	2 cut(s)	640, 778
SspMI	CTAG	1 cut(s)	15
SstI	GAGCTC	1 cut(s)	744
StuI	AGGCCT	1 cut(s)	162
StyD4I	CCNGG	3 cut(s)	263, 327, 496
StyI	CCWWGG	1 cut(s)	928
TaaI	ACNGT	2 cut(s)	810, 916
TaqI	TCGA	4 cut(s)	126, 372, 635, 801
TasI	AATT	8 cut(s)	29, 89, 291, 459, 764, 971, 1057, 1084
TfiI	GAWTC	3 cut(s)	61, 196, 241
Tru1I	TTAA	3 cut(s)	549, 830, 1182
Tru9I	TTAA	3 cut(s)	549, 830, 1182
TscAI	CASTG	3 cut(s)	813, 919, 1012
TseFI	GTSAC	2 cut(s)	395, 1147
TseI	GCWGC	5 cut(s)	625, 900, 1036, 1171, 1213
Tsp45I	GTSAC	2 cut(s)	395, 1147
TspDTI	ATGAA	6 cut(s)	74, 407, 582, 591, 719, 1082
TspRI	CASTG	3 cut(s)	813, 919, 1012
Van91I	CCANNNNNTGG	1 cut(s)	334
XapI	RAATTY	4 cut(s)	89, 291, 459, 971
XceI	RCATGY	1 cut(s)	517
XcmI	CCANNNNNNNNNTGG	2 cut(s)	485, 1012
XspI	CTAG	1 cut(s)	15
Zsp2I	ATGCAT	1 cut(s)	172

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.