MD09G1189100.v1.1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000799
Physical Location & Seq
Forward (+)
16512562 .. 16515929
3368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1189100.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGAGTGAGCTGCTCTCAACTGATGGGCAAGATTTCTTCACATCGTATGATGAAGTTTTTGAAAGTTTTGATGCCATGGGATTGCAGGAAAATCTGCTGAGAGGCATTTATGCTTATGGTTTTGAGAAGCCTTCTGCAATTCAGCAAAGGGGAATTGTTCCATTCTGCAAAGGCCTTGATGTGATTCAACAGGCTCAGTCTGGAACCGGGAAAACAGCAACCTTTTGCTCAGGGATTTTGCAGCAACTTGATTATGGTGTTGTCCAGTGCCAGGCCTTGGTTTTGGCTCCTACCAGGGAGTTGGCACAGCAGATTGAGAAGGTTATGCGAGCACTTGGTGACTACCTTGGCGTTAAGGTTCATGCTTGTGTTGGTGGGACAAGTGTTCGTGAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTTGTTGTTGGAACCCCTGGTCGTGTGTTTGACATGTTGAGGAGGCAGTCGCTTCGCCCAGATTACATTAAGATGTTTGTATTGGATGAGGCTGATGAAATGCTTTCTCGTGGTTTTAAGGATCAGATCTACGACATTTTCCAGCTTTTGCCATCAAAGGTTCAGGTTGGGGTGTTCTCTGCTACCATGCCACCTGAAGCCCTTGAAATCACTAGAAAGTTTATGAACAAACCTGTGAGGATTTTGGTAAAGCGTGATGAGCTCACCCTTGAGGGTATCAAGCAGTTCTATGTCAATGTCGATAAAGAAGAATGGAAGCTCGAGACCCTTTGTGATTTGTATGAGACTCTGGCCATCACCCAGAGTGTCATTTTTGTTAACACCAGGCGCAAGGTGGACTGGCTTACCGACAAGATGAGAAGCCGTGACCACACTGTCTCTGCCACCCATGGTGACATGGACCAGAACACTCGTGATATCATCATGCGTGAATTCCGATCAGGCTCATCCCGTGTTCTCATCACCACTGATCTCTTGGCTCGTGGTATTGATGTGCAGCAGGTATCTCTCGTAATCAACTATGATTTGCCGACTCAACCTGAAAACTACCTTCATCGTATTGGACGAAGTGGTAGATTTGGAAGGAAGGGTGTTGCTATTAACTTTGTGACAAAAGACGACGAGAGAATGCTCTATGACATCCAGAGGTTTTACAACGTGGTGATTGAGGAGCTCCCATCGAATGTGGCCGATCTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

395

Amino Acids

44.82

Weight (kDa)

5.58

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 46 - 208 6.6e-43 DEAD/DEAH box helicase
ResIII PF04851 64 - 204 6.7e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 247 - 355 7.8e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 973
AccB7I CCANNNNNTGG 1 cut(s) 277
AclWI GGATC 2 cut(s) 402, 552
AcoI YGGCCR 2 cut(s) 774, 1170
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 1 cut(s) 639
AdeI CACNNNGTG 1 cut(s) 338
AfiI CCNNNNNNNGG 2 cut(s) 271, 277
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 4 cut(s) 62, 188, 410, 629
AjnI CCWGG 4 cut(s) 270, 293, 439, 806
AluBI AGCT 6 cut(s) 10, 413, 568, 685, 742, 1156
AluI AGCT 6 cut(s) 10, 413, 568, 685, 742, 1156
Alw21I GWGCWC 3 cut(s) 334, 687, 1158
Alw26I GTCTC 3 cut(s) 740, 761, 865
AlwI GGATC 2 cut(s) 402, 552
Ama87I CYCGRG 1 cut(s) 743
AoxI GGCC 4 cut(s) 172, 273, 774, 1170
ApeKI GCWGC 3 cut(s) 10, 241, 979
ApoI RAATTY 1 cut(s) 914
AspLEI GCGC 2 cut(s) 402, 813
AspS9I GGNCC 1 cut(s) 883
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 6 cut(s) 350, 679, 772, 887, 937, 1156
AvaI CYCGRG 1 cut(s) 743
AvaII GGWCC 1 cut(s) 883
BalI TGGCCA 1 cut(s) 776
BanII GRGCYC 2 cut(s) 687, 1158
BauI CACGAG 3 cut(s) 531, 894, 963
Bbv12I GWGCWC 3 cut(s) 334, 687, 1158
BbvI GCAGC 2 cut(s) 253, 991
BccI CCATC 4 cut(s) 17, 583, 785, 1168
BceAI ACGGC 1 cut(s) 831
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BciT130I CCWGG 4 cut(s) 272, 295, 441, 808
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 3 cut(s) 740, 761, 865
BfaI CTAG 1 cut(s) 636
BfuAI ACCTGC 1 cut(s) 973
BglII AGATCT 1 cut(s) 549
BisI GCNGC 3 cut(s) 11, 242, 980
BlsI GCNGC 3 cut(s) 12, 243, 981
Bme1390I CCNGG 5 cut(s) 208, 272, 295, 441, 808
Bme18I GGWCC 1 cut(s) 883
BmeT110I CYCGRG 1 cut(s) 743
BmgT120I GGNCC 1 cut(s) 883
BmiI GGNNCC 3 cut(s) 205, 288, 436
BmrFI CCNGG 5 cut(s) 208, 272, 295, 441, 808
BmsI GCATC 1 cut(s) 61
BplI GAGNNNNNCTC 1 cut(s) 31
Bpu10I CCTNAGC 1 cut(s) 229
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 208
BsaI GGTCTC 1 cut(s) 740
BsaJI CCNNGG 6 cut(s) 75, 276, 294, 346, 439, 871
Bsc4I CCNNNNNNNGG 2 cut(s) 271, 277
Bse1I ACTGG 2 cut(s) 265, 827
BseBI CCWGG 4 cut(s) 272, 295, 441, 808
BseDI CCNNGG 6 cut(s) 75, 276, 294, 346, 439, 871
BseGI GGATG 3 cut(s) 514, 929, 1122
BseLI CCNNNNNNNGG 2 cut(s) 271, 277
BseMII CTCAG 3 cut(s) 89, 209, 243
BseNI ACTGG 2 cut(s) 265, 827
BseRI GAGGAG 2 cut(s) 478, 1166
BseXI GCAGC 2 cut(s) 253, 991
BsgI GTGCAG 1 cut(s) 998
BshFI GGCC 4 cut(s) 174, 275, 776, 1172
BsiHKAI GWGCWC 3 cut(s) 334, 687, 1158
BsiHKCI CYCGRG 1 cut(s) 743
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 2 cut(s) 271, 277
BsmAI GTCTC 3 cut(s) 740, 761, 865
BsmFI GGGAC 1 cut(s) 391
BsmI GAATGC 2 cut(s) 402, 1116
BsnI GGCC 4 cut(s) 174, 275, 776, 1172
Bso31I GGTCTC 1 cut(s) 740
BsoBI CYCGRG 1 cut(s) 743
Bsp1286I GDGCHC 3 cut(s) 334, 687, 1158
Bsp143I GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
Bsp19I CCATGG 2 cut(s) 75, 871
BspANI GGCC 4 cut(s) 174, 275, 776, 1172
BspCNI CTCAG 3 cut(s) 90, 208, 242
BspLI GGNNCC 3 cut(s) 205, 288, 436
BspMI ACCTGC 1 cut(s) 973
BspPI GGATC 2 cut(s) 402, 552
BspTNI GGTCTC 1 cut(s) 740
BsrI ACTGG 2 cut(s) 265, 827
BssECI CCNNGG 6 cut(s) 75, 276, 294, 346, 439, 871
BssMI GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
BssSI CACGAG 3 cut(s) 531, 894, 963
BssT1I CCWWGG 4 cut(s) 75, 276, 346, 871
Bst2BI CACGAG 3 cut(s) 531, 894, 963
Bst2UI CCWGG 4 cut(s) 272, 295, 441, 808
Bst4CI ACNGT 1 cut(s) 859
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 3 cut(s) 98, 195, 229
BstDSI CCRYGG 2 cut(s) 75, 871
BstF5I GGATG 3 cut(s) 514, 929, 1122
BstHHI GCGC 2 cut(s) 402, 813
BstKTI GATC 6 cut(s) 397, 547, 552, 923, 955, 1177
BstMAI GTCTC 3 cut(s) 740, 761, 865
BstMBI GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
BstMWI GCNNNNNNNGC 1 cut(s) 682
BstNI CCWGG 4 cut(s) 272, 295, 441, 808
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 5 cut(s) 206, 270, 293, 439, 806
BstV1I GCAGC 2 cut(s) 253, 991
BstX2I RGATCY 1 cut(s) 549
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 1 cut(s) 549
BsuRI GGCC 4 cut(s) 174, 275, 776, 1172
BtgI CCRYGG 2 cut(s) 75, 871
BtsCI GGATG 3 cut(s) 514, 929, 1122
BtsIMutI CAGTG 3 cut(s) 272, 855, 948
BveI ACCTGC 1 cut(s) 973
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 2 cut(s) 402, 813
Cfr13I GGNCC 1 cut(s) 883
CviAII CATG 8 cut(s) 76, 362, 422, 457, 610, 872, 880, 907
DdeI CTNAG 3 cut(s) 98, 195, 229
DpnI GATC 6 cut(s) 396, 546, 551, 922, 954, 1176
DpnII GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
DraIII CACNNNGTG 1 cut(s) 338
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 2 cut(s) 774, 1170
Ecl136II GAGCTC 2 cut(s) 685, 1156
Eco130I CCWWGG 4 cut(s) 75, 276, 346, 871
Eco147I AGGCCT 2 cut(s) 174, 275
Eco24I GRGCYC 2 cut(s) 687, 1158
Eco31I GGTCTC 1 cut(s) 740
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 1 cut(s) 883
Eco53kI GAGCTC 2 cut(s) 685, 1156
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 1 cut(s) 743
EcoICRI GAGCTC 2 cut(s) 685, 1156
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 4 cut(s) 270, 293, 439, 806
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 4 cut(s) 75, 276, 346, 871
EcoT38I GRGCYC 2 cut(s) 687, 1158
ErhI CCWWGG 4 cut(s) 75, 276, 346, 871
FaeI CATG 8 cut(s) 79, 365, 425, 460, 613, 875, 883, 910
FaqI GGGAC 1 cut(s) 391
FatI CATG 8 cut(s) 75, 361, 421, 456, 609, 871, 879, 906
Fnu4HI GCNGC 3 cut(s) 11, 242, 980
FokI GGATG 3 cut(s) 521, 916, 1109
FriOI GRGCYC 2 cut(s) 687, 1158
Fsp4HI GCNGC 3 cut(s) 11, 242, 980
FspBI CTAG 1 cut(s) 636
GlaI GCGC 2 cut(s) 401, 812
GluI GCNGC 3 cut(s) 11, 242, 980
HaeIII GGCC 4 cut(s) 174, 275, 776, 1172
HapII CCGG 1 cut(s) 207
HhaI GCGC 2 cut(s) 402, 813
Hin1II CATG 8 cut(s) 79, 365, 425, 460, 613, 875, 883, 910
Hin6I GCGC 2 cut(s) 400, 811
HinP1I GCGC 2 cut(s) 400, 811
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 3 cut(s) 184, 769, 1015
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 1 cut(s) 207
HphI GGTGA 6 cut(s) 350, 679, 772, 887, 937, 1156
Hpy166II GTNNAC 2 cut(s) 802, 820
Hpy188I TCNGA 3 cut(s) 549, 920, 1184
Hpy188III TCNNGA 5 cut(s) 201, 389, 745, 896, 1126
Hpy8I GTNNAC 2 cut(s) 802, 820
Hpy99I CGWCG 1 cut(s) 1106
HpyAV CCTTC 5 cut(s) 141, 313, 1043, 1059, 1063
HpyCH4III ACNGT 1 cut(s) 859
HpyCH4IV ACGT 1 cut(s) 1140
HpyCH4V TGCA 5 cut(s) 85, 137, 168, 241, 979
HpyF10VI GCNNNNNNNGC 1 cut(s) 682
HpyF3I CTNAG 3 cut(s) 98, 195, 229
HpySE526I ACGT 1 cut(s) 1140
Hsp92II CATG 8 cut(s) 79, 365, 425, 460, 613, 875, 883, 910
HspAI GCGC 2 cut(s) 400, 811
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
LmnI GCTCC 3 cut(s) 292, 1153, 1161
Lsp1109I GCAGC 2 cut(s) 253, 991
LweI GCATC 1 cut(s) 61
MaeI CTAG 1 cut(s) 636
MaeII ACGT 1 cut(s) 1140
MaeIII GTNAC 4 cut(s) 338, 848, 875, 1090
MalI GATC 6 cut(s) 396, 546, 551, 922, 954, 1176
MboI GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
MboII GAAGA 4 cut(s) 28, 398, 743, 1169
MflI RGATCY 1 cut(s) 549
MhlI GDGCHC 3 cut(s) 334, 687, 1158
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 3 cut(s) 138, 153, 914
MluNI TGGCCA 1 cut(s) 776
MlyI GAGTC 2 cut(s) 763, 1009
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 9 cut(s) 95, 385, 456, 459, 505, 654, 688, 1122, 1144
Mox20I TGGCCA 1 cut(s) 776
MscI TGGCCA 1 cut(s) 776
MseI TTAA 5 cut(s) 354, 492, 540, 801, 1083
MslI CAYNNNNRTG 2 cut(s) 366, 494
Msp20I TGGCCA 1 cut(s) 776
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 5 cut(s) 208, 272, 295, 441, 808
Mva1269I GAATGC 2 cut(s) 402, 1116
MvaI CCWGG 4 cut(s) 272, 295, 441, 808
MwoI GCNNNNNNNGC 1 cut(s) 682
NciI CCSGG 1 cut(s) 208
NcoI CCATGG 2 cut(s) 75, 871
NdeII GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
NlaIII CATG 8 cut(s) 79, 365, 425, 460, 613, 875, 883, 910
NlaIV GGNNCC 3 cut(s) 205, 288, 436
NmuCI GTSAC 4 cut(s) 338, 848, 875, 1090
NspI RCATGY 1 cut(s) 460
PaeR7I CTCGAG 1 cut(s) 743
PceI AGGCCT 2 cut(s) 174, 275
PciI ACATGT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 1045
PctI GAATGC 2 cut(s) 402, 1116
PfeI GAWTC 1 cut(s) 184
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 3 cut(s) 12, 243, 981
PleI GAGTC 2 cut(s) 763, 1009
PpsI GAGTC 2 cut(s) 763, 1009
PscI ACATGT 1 cut(s) 456
Psp124BI GAGCTC 2 cut(s) 687, 1158
Psp6I CCWGG 4 cut(s) 270, 293, 439, 806
PspGI CCWGG 4 cut(s) 270, 293, 439, 806
PspN4I GGNNCC 3 cut(s) 205, 288, 436
PspPI GGNCC 1 cut(s) 883
PsuI RGATCY 1 cut(s) 549
RseI CAYNNNNRTG 2 cut(s) 366, 494
SacI GAGCTC 2 cut(s) 687, 1158
SaqAI TTAA 5 cut(s) 354, 492, 540, 801, 1083
SatI GCNGC 3 cut(s) 11, 242, 980
Sau3AI GATC 6 cut(s) 394, 544, 549, 920, 952, 1174
Sau96I GGNCC 1 cut(s) 883
SchI GAGTC 2 cut(s) 763, 1009
ScrFI CCNGG 5 cut(s) 208, 272, 295, 441, 808
SduI GDGCHC 3 cut(s) 334, 687, 1158
SfaNI GCATC 1 cut(s) 61
Sfr274I CTCGAG 1 cut(s) 743
SinI GGWCC 1 cut(s) 883
SlaI CTCGAG 1 cut(s) 743
SmiMI CAYNNNNRTG 2 cut(s) 366, 494
SmlI CTYRAG 2 cut(s) 692, 743
SmoI CTYRAG 2 cut(s) 692, 743
Sse9I AATT 3 cut(s) 138, 153, 914
SseBI AGGCCT 2 cut(s) 174, 275
SspMI CTAG 1 cut(s) 636
SstI GAGCTC 2 cut(s) 687, 1158
StuI AGGCCT 2 cut(s) 174, 275
StyD4I CCNGG 5 cut(s) 206, 270, 293, 439, 806
StyI CCWWGG 4 cut(s) 75, 276, 346, 871
TaaI ACNGT 1 cut(s) 859
TaiI ACGT 1 cut(s) 1143
TaqI TCGA 3 cut(s) 723, 744, 1163
TasI AATT 3 cut(s) 138, 153, 914
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 5 cut(s) 354, 492, 540, 801, 1083
Tru9I TTAA 5 cut(s) 354, 492, 540, 801, 1083
TscAI CASTG 3 cut(s) 272, 862, 955
TseFI GTSAC 4 cut(s) 338, 848, 875, 1090
TseI GCWGC 3 cut(s) 10, 241, 979
Tsp45I GTSAC 4 cut(s) 338, 848, 875, 1090
TspDTI ATGAA 6 cut(s) 66, 350, 410, 534, 662, 1025
TspRI CASTG 3 cut(s) 272, 862, 955
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 883
XapI RAATTY 1 cut(s) 914
XceI RCATGY 1 cut(s) 460
XcmI CCANNNNNNNNNTGG 2 cut(s) 814, 955
XhoI CTCGAG 1 cut(s) 743
XspI CTAG 1 cut(s) 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.