Rroxscaffold_2G00101330

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000799
Physical Location & Seq
Forward (+)
23317019 .. 23319923
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00101330.1

Sequence Viewer

Length: 1242 bp
ATGGCAGGTCTTGCTCCCGAAGGTGCACAGTTTGATGCTAAACAGTATGATTCTAAGATGAGTGAGATCCTCTCATCTGAAGGCCAAGAGTTCTTCACATCATATGATGAGGTTCATGACAGTTTTGATGCCATGGGTTTACAAGAAAATCTTTTAAGGGGAATATATGCTTATGGATTTGAGAAGCCTTCTGCGATTCAGCAAAGAGGAATTGTTCCTTTCTGTAAAGGTTTGGATGTGATTCAACAAGCTCAGTCAGGAACAGGCAAAACAGCAACCTTTTGCTCTGGAATTTTGCAGCAACTTGACTATGGTTTGGTCCAGTGCCAGGCTTTGGTGTTGGCACCTACCAGGGAGTTAGCTCAGCAGATTGAGAAGGTTATGCGGGCGCTTGGAGACTATCTCGGCGTGAAGGTTCATGCTTGCGTAGGTGGGACTAGTGTTCGTGAGGATCAGCGAATTCTTCAGACTGGTGTGCATGTGGTGGTTGGCACTCCTGGACGTGTGTTTGACATGTTGCGGAGGCAATCTCTTCGTGCAGATTACATTAAGATGTTTGTATTGGATGAAGCCGATGAAATGCTATCTAGGGGTTTCAAGGATCAGATCTATGACATTTTTCAGCTTCTGCCTTCAAAAGTTCAAGTTGGTGTGTTCTCTGCTACTATGCCTCCTGAAGCCCTTGAGATCACCAGAAAGTTTATGAACAAACCTGTTAGGATTTTGGTTAAGCGTGATGAGCTCACCCTTGAGGGAATCAAGCAATTTTATGTTAATGTCGATAAGGAAGAATGGAAGCTTGAGACACTTTGCGATCTCTATGAGACCCTTGCAATCACTCAGAGTGTCATTTTTGTTAATACAAGGCGCAAAGTGGATTGGCTTACAGATAAGATGCGAAGCCGTGACCACACTGTGTCTGCTACCCATGGTGATATGGACCAGAACCAGCGTGACATTATCATGCGTGAATTCCGATCTGGTTCCTCCCGTGTTCTCATCACTACTGATCTGTTGGCACGTGGTATTGATGTGCAGCAAGTGTCGCTTGTAATTAATTATGATTTGCCGACTCAACCAGAAAACTACCTTCATCGTATTGGACGAAGTGGGCGATTTGGAAGAAAAGGTGTTGCCATCAATTTCGTGACTAGGGATGACGATAGAATGCTATATGACATTCAGAGGTTTTATAATGTGGTGATTGAGGAGCTGCCTTCCAACGTGGCCGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

413

Amino Acids

46.88

Weight (kDa)

5.56

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 65 - 227 1.7e-43 DEAD/DEAH box helicase
ResIII PF04851 83 - 223 8.1e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 266 - 374 2.9e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1194
AccB1I GGYRCC 1 cut(s) 343
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 2 cut(s) 385, 520
AclWI GGATC 3 cut(s) 61, 459, 609
AcoI YGGCCR 1 cut(s) 1227
AcsI RAATTY 3 cut(s) 291, 459, 971
AcuI CTGAAG 3 cut(s) 99, 449, 696
AcvI CACGTG 1 cut(s) 1022
AdeI CACNNNGTG 1 cut(s) 916
AfiI CCNNNNNNNGG 2 cut(s) 328, 334
AflIII ACRYGT 2 cut(s) 502, 513
AgsI TTSAA 4 cut(s) 245, 598, 636, 644
AhlI ACTAGT 1 cut(s) 437
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 3 cut(s) 327, 350, 496
AluBI AGCT 6 cut(s) 251, 362, 625, 742, 799, 1213
AluI AGCT 6 cut(s) 251, 362, 625, 742, 799, 1213
Alw21I GWGCWC 2 cut(s) 28, 744
Alw26I GTCTC 3 cut(s) 390, 797, 818
Alw44I GTGCAC 1 cut(s) 24
AlwI GGATC 3 cut(s) 61, 459, 609
AlwNI CAGNNNCTG 1 cut(s) 628
AoxI GGCC 2 cut(s) 82, 1227
ApaLI GTGCAC 1 cut(s) 24
ApeKI GCWGC 3 cut(s) 298, 1036, 1213
ApoI RAATTY 3 cut(s) 291, 459, 971
AseI ATTAAT 1 cut(s) 1056
AspLEI GCGC 2 cut(s) 391, 870
AspS9I GGNCC 2 cut(s) 319, 940
AsuHPI GGTGA 4 cut(s) 682, 736, 944, 1213
AvaII GGWCC 2 cut(s) 319, 940
BaeGI GKGCMC 1 cut(s) 28
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 744
BbrPI CACGTG 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 28, 744
BbvI GCAGC 3 cut(s) 310, 1048, 1200
BccI CCATC 1 cut(s) 1145
BceAI ACGGC 1 cut(s) 888
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 3 cut(s) 329, 352, 498
BcoDI GTCTC 3 cut(s) 390, 797, 818
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 3 cut(s) 438, 588, 1152
BfoI RGCGCY 1 cut(s) 392
BglII AGATCT 1 cut(s) 606
BisI GCNGC 3 cut(s) 299, 1037, 1214
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 3 cut(s) 300, 1038, 1215
Bme1390I CCNGG 3 cut(s) 329, 352, 498
Bme18I GGWCC 2 cut(s) 319, 940
BmgBI CACGTC 1 cut(s) 503
BmgT120I GGNCC 2 cut(s) 319, 940
BmiI GGNNCC 2 cut(s) 345, 985
BmrFI CCNGG 3 cut(s) 329, 352, 498
BmsI GCATC 3 cut(s) 25, 118, 885
BplI GAGNNNNNCTC 6 cut(s) 56, 88, 387, 419, 514, 546
Bpu1102I GCTNAGC 1 cut(s) 363
BpuEI CTTGAG 3 cut(s) 704, 770, 821
BsaAI YACGTR 1 cut(s) 1022
BsaI GGTCTC 1 cut(s) 818
BsaJI CCNNGG 3 cut(s) 132, 351, 928
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 334
Bse1I ACTGG 2 cut(s) 322, 475
BseBI CCWGG 3 cut(s) 329, 352, 498
BseDI CCNNGG 3 cut(s) 132, 351, 928
BseGI GGATG 3 cut(s) 241, 571, 1162
BseLI CCNNNNNNNGG 2 cut(s) 328, 334
BseMII CTCAG 3 cut(s) 266, 377, 854
BseNI ACTGG 2 cut(s) 322, 475
BseRI GAGGAG 1 cut(s) 1223
BseSI GKGCMC 1 cut(s) 28
BseXI GCAGC 3 cut(s) 310, 1048, 1200
BsgI GTGCAG 2 cut(s) 558, 1055
BshFI GGCC 2 cut(s) 84, 1229
BshNI GGYRCC 1 cut(s) 343
BsiHKAI GWGCWC 2 cut(s) 28, 744
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 2 cut(s) 328, 334
BsmAI GTCTC 3 cut(s) 390, 797, 818
BsmFI GGGAC 1 cut(s) 448
BsmI GAATGC 1 cut(s) 1173
BsnI GGCC 2 cut(s) 84, 1229
Bso31I GGTCTC 1 cut(s) 818
Bsp1286I GDGCHC 2 cut(s) 28, 744
Bsp143I GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
Bsp1720I GCTNAGC 1 cut(s) 363
Bsp19I CCATGG 2 cut(s) 132, 928
BspACI CCGC 2 cut(s) 385, 520
BspANI GGCC 2 cut(s) 84, 1229
BspCNI CTCAG 3 cut(s) 265, 376, 853
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 345, 985
BspPI GGATC 3 cut(s) 61, 459, 609
BspT107I GGYRCC 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 818
BsrI ACTGG 2 cut(s) 322, 475
BssECI CCNNGG 3 cut(s) 132, 351, 928
BssMI GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
BssT1I CCWWGG 2 cut(s) 132, 928
Bst2UI CCWGG 3 cut(s) 329, 352, 498
Bst4CI ACNGT 4 cut(s) 30, 45, 122, 916
Bst6I CTCTTC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 1022
BstC8I GCNNGC 2 cut(s) 387, 424
BstDEI CTNAG 4 cut(s) 54, 252, 363, 840
BstDSI CCRYGG 2 cut(s) 132, 928
BstF5I GGATG 3 cut(s) 241, 571, 1162
BstH2I RGCGCY 1 cut(s) 392
BstHHI GCGC 2 cut(s) 391, 870
BstKTI GATC 9 cut(s) 69, 454, 604, 609, 690, 817, 980, 1012, 1234
BstMAI GTCTC 3 cut(s) 390, 797, 818
BstMBI GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
BstMWI GCNNNNNNNGC 3 cut(s) 11, 739, 1045
BstNI CCWGG 3 cut(s) 329, 352, 498
BstNSI RCATGY 2 cut(s) 482, 517
BstSCI CCNGG 3 cut(s) 327, 350, 496
BstSLI GKGCMC 1 cut(s) 28
BstV1I GCAGC 3 cut(s) 310, 1048, 1200
BstX2I RGATCY 2 cut(s) 66, 606
BstYI RGATCY 2 cut(s) 66, 606
BsuRI GGCC 2 cut(s) 84, 1229
BtgI CCRYGG 2 cut(s) 132, 928
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 3 cut(s) 241, 571, 1162
BtsIMutI CAGTG 2 cut(s) 329, 912
Cac8I GCNNGC 2 cut(s) 387, 424
CaiI CAGNNNCTG 1 cut(s) 628
CciI TCATGA 1 cut(s) 115
CfoI GCGC 2 cut(s) 391, 870
Cfr13I GGNCC 2 cut(s) 319, 940
CviAII CATG 7 cut(s) 116, 133, 419, 479, 514, 929, 964
DdeI CTNAG 4 cut(s) 54, 252, 363, 840
DpnI GATC 9 cut(s) 68, 453, 603, 608, 689, 816, 979, 1011, 1233
DpnII GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
DraIII CACNNNGTG 1 cut(s) 916
EaeI YGGCCR 1 cut(s) 1227
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 2 cut(s) 132, 928
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 818
Eco47I GGWCC 2 cut(s) 319, 940
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 3 cut(s) 99, 449, 696
Eco72I CACGTG 1 cut(s) 1022
EcoICRI GAGCTC 1 cut(s) 742
EcoRI GAATTC 2 cut(s) 459, 971
EcoRII CCWGG 3 cut(s) 327, 350, 496
EcoT14I CCWWGG 2 cut(s) 132, 928
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 2 cut(s) 132, 928
FaeI CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 1032, 1064
FaqI GGGAC 1 cut(s) 448
FatI CATG 7 cut(s) 115, 132, 418, 478, 513, 928, 963
FauI CCCGC 1 cut(s) 378
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 299, 1037, 1214
FokI GGATG 3 cut(s) 248, 578, 1169
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 3 cut(s) 299, 1037, 1214
FspBI CTAG 3 cut(s) 438, 588, 1152
GlaI GCGC 2 cut(s) 390, 869
GluI GCNGC 3 cut(s) 299, 1037, 1214
HaeII RGCGCY 1 cut(s) 392
HaeIII GGCC 2 cut(s) 84, 1229
HhaI GCGC 2 cut(s) 391, 870
Hin1II CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
Hin6I GCGC 2 cut(s) 389, 868
HinP1I GCGC 2 cut(s) 389, 868
HindIII AAGCTT 1 cut(s) 797
HinfI GANTC 5 cut(s) 50, 196, 241, 756, 1072
HphI GGTGA 4 cut(s) 682, 736, 944, 1213
Hpy166II GTNNAC 2 cut(s) 26, 140
Hpy188I TCNGA 6 cut(s) 79, 468, 606, 843, 977, 1185
Hpy188III TCNNGA 7 cut(s) 17, 116, 258, 288, 446, 674, 1147
Hpy8I GTNNAC 2 cut(s) 26, 140
HpyAV CCTTC 8 cut(s) 14, 74, 198, 370, 406, 642, 1100, 1227
HpyCH4III ACNGT 4 cut(s) 30, 45, 122, 916
HpyCH4IV ACGT 3 cut(s) 502, 1021, 1224
HpyCH4V TGCA 6 cut(s) 26, 298, 478, 539, 833, 1036
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 739, 1045
HpyF3I CTNAG 4 cut(s) 54, 252, 363, 840
HpySE526I ACGT 3 cut(s) 502, 1021, 1224
Hsp92II CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
HspAI GCGC 2 cut(s) 389, 868
Kzo9I GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
LmnI GCTCC 2 cut(s) 19, 1210
Lsp1109I GCAGC 3 cut(s) 310, 1048, 1200
LweI GCATC 3 cut(s) 25, 118, 885
MaeI CTAG 3 cut(s) 438, 588, 1152
MaeII ACGT 3 cut(s) 502, 1021, 1224
MaeIII GTNAC 3 cut(s) 905, 953, 1147
MalI GATC 9 cut(s) 68, 453, 603, 608, 689, 816, 979, 1011, 1233
MboI GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
MboII GAAGA 6 cut(s) 85, 455, 524, 800, 1134, 1226
MflI RGATCY 2 cut(s) 66, 606
MhlI GDGCHC 2 cut(s) 28, 744
MluCI AATT 8 cut(s) 210, 291, 459, 764, 971, 1053, 1057, 1141
MlyI GAGTC 1 cut(s) 1066
MseI TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
MslI CAYNNNNRTG 2 cut(s) 551, 962
MspR9I CCNGG 3 cut(s) 329, 352, 498
Mva1269I GAATGC 1 cut(s) 1173
MvaI CCWGG 3 cut(s) 329, 352, 498
MwoI GCNNNNNNNGC 3 cut(s) 11, 739, 1045
NcoI CCATGG 2 cut(s) 132, 928
NdeI CATATG 1 cut(s) 103
NdeII GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
NlaIII CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
NlaIV GGNNCC 2 cut(s) 345, 985
NmeAIII GCCGAG 1 cut(s) 384
NmuCI GTSAC 3 cut(s) 905, 953, 1147
NspI RCATGY 2 cut(s) 482, 517
PagI TCATGA 1 cut(s) 115
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 2 cut(s) 1102, 1111
PctI GAATGC 1 cut(s) 1173
PfeI GAWTC 4 cut(s) 50, 196, 241, 756
PflMI CCANNNNNTGG 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 3 cut(s) 300, 1038, 1215
PleI GAGTC 1 cut(s) 1066
PmaCI CACGTG 1 cut(s) 1022
PmlI CACGTG 1 cut(s) 1022
PpsI GAGTC 1 cut(s) 1066
Ppu21I YACGTR 1 cut(s) 1022
PscI ACATGT 1 cut(s) 513
PshBI ATTAAT 1 cut(s) 1056
PsiI TTATAA 1 cut(s) 1194
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 3 cut(s) 327, 350, 496
PspCI CACGTG 1 cut(s) 1022
PspGI CCWGG 3 cut(s) 327, 350, 496
PspN4I GGNNCC 2 cut(s) 345, 985
PspPI GGNCC 2 cut(s) 319, 940
PstNI CAGNNNCTG 1 cut(s) 628
PsuI RGATCY 2 cut(s) 66, 606
RseI CAYNNNNRTG 2 cut(s) 551, 962
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
SatI GCNGC 3 cut(s) 299, 1037, 1214
Sau3AI GATC 9 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009, 1231
Sau96I GGNCC 2 cut(s) 319, 940
SchI GAGTC 1 cut(s) 1066
ScrFI CCNGG 3 cut(s) 329, 352, 498
SduI GDGCHC 2 cut(s) 28, 744
SfaNI GCATC 3 cut(s) 25, 118, 885
SinI GGWCC 2 cut(s) 319, 940
SmiMI CAYNNNNRTG 2 cut(s) 551, 962
SmlI CTYRAG 3 cut(s) 683, 749, 800
SmoI CTYRAG 3 cut(s) 683, 749, 800
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 8 cut(s) 210, 291, 459, 764, 971, 1053, 1057, 1141
SsiI CCGC 2 cut(s) 385, 520
SspMI CTAG 3 cut(s) 438, 588, 1152
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 327, 350, 496
StyI CCWWGG 2 cut(s) 132, 928
TaaI ACNGT 4 cut(s) 30, 45, 122, 916
TaiI ACGT 3 cut(s) 505, 1024, 1227
TaqI TCGA 1 cut(s) 780
TasI AATT 8 cut(s) 210, 291, 459, 764, 971, 1053, 1057, 1141
TfiI GAWTC 4 cut(s) 50, 196, 241, 756
Tru1I TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
Tru9I TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
TscAI CASTG 2 cut(s) 329, 919
TseFI GTSAC 3 cut(s) 905, 953, 1147
TseI GCWGC 3 cut(s) 298, 1036, 1213
Tsp45I GTSAC 3 cut(s) 905, 953, 1147
TspDTI ATGAA 6 cut(s) 104, 407, 582, 591, 719, 1082
TspRI CASTG 2 cut(s) 329, 919
Van91I CCANNNNNTGG 1 cut(s) 334
VneI GTGCAC 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 319, 940
VspI ATTAAT 1 cut(s) 1056
XapI RAATTY 3 cut(s) 291, 459, 971
XceI RCATGY 2 cut(s) 482, 517
XspI CTAG 3 cut(s) 438, 588, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.