Rroxscaffold_2G00147800

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000799
Physical Location & Seq
Forward (+)
85496251 .. 85498148
1898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147800.1

Sequence Viewer

Length: 1098 bp
ATGATTGCACACATGATTATTCTCTATCCAGGTTTTGAGAAACCCTCTGCCATTCAGCAAAGGGGAATTGTCCCCTTTATTAAGGGTCTTGATGTGATTCAGCAGGCTCAATCTGGAACTGGGAAGACAGCGACCTTCTGTTCTGGTATTCTTCAACAGCTTGATTATAGTTTGACAGATTGCCAGGGCTTGGTTCTGGCACCCACTCGAGAGCTTGCCCAACAAATAGAGAAGGTCATGAGGGCCCTTGGAGATTATCTTGGTGTTAAGGTGCATGCATGTGTTGGTGGAACTAGTGTTCGTGAAGACCAACGTATTCTGTCAAATGGAGTTCATGCTGTTGTTGGAACTCCAGGTCGTGTTTTTGATATGCTAAGAAGACAGTCACTTCGCCCTGATCATATCAAGATGTTTGTTCTTGATGAGGCTGATGAAATGCTTTCTCGGGGTTTCAAGGACCAGATCTATGATATATTCCAGCTTCTCCCACCAAAGATTCAGGTTGGTGTGTTTTCTGCCACCATGCCACCTGAGGCCCTGGAGATCACAAGGAAGTTCATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAACTCACCCTGGAAGGAATTAAGCAGTTTTATGTCAATGTGGACAAGGAGGAGTGGAAGCTTGACACTTTGTGTGATCTTTATGAAACCTTGGCCATTACCCAGAGTGTGATTTTCGTGAATACTCGACGCAAGGTTGATTGGTTGACCGACAAAATGAGAAGCCGGGACCATACAGTCTCTGCAACCCATGGAGACATGGACCAGAACAACAGAGACATCATCATGAGGGAATTCCGTTCTGGGTCATCTCGTGTCTTGATCACCACCGATCTGTTGGCTCGTGGTATTGATGTACAACAAGTTTCCCTTGTTATTAACTATGATCTGCCTACCCAGCCTGAGAACTACCTCCATAGAATTGGTCGTAGTGGAAGATTTGGTAGGAAGGGAGTTGCAATCAACTTTGTTACAAAGGATGATGAGAGAATGCTGTTTGATATCCAGAAGTTCTACAATGTGGTAGTAGAGGAGCTGCCTTCAAATGTTGCTGATCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

365

Amino Acids

41.46

Weight (kDa)

7.06

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 17 - 179 3.1e-43 DEAD/DEAH box helicase
ResIII PF04851 35 - 175 1.1e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 218 - 326 6.6e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 770
AccB1I GGYRCC 1 cut(s) 199
AccB7I CCANNNNNTGG 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 687
AcsI RAATTY 1 cut(s) 827
AdeI CACNNNGTG 1 cut(s) 666
AfaI GTAC 1 cut(s) 891
AfiI CCNNNNNNNGG 1 cut(s) 190
AgsI TTSAA 3 cut(s) 155, 454, 1077
AhlI ACTAGT 1 cut(s) 293
AjnI CCWGG 5 cut(s) 28, 183, 352, 537, 603
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
AluBI AGCT 5 cut(s) 160, 214, 481, 655, 1069
AluI AGCT 5 cut(s) 160, 214, 481, 655, 1069
Alw26I GTCTC 3 cut(s) 778, 783, 804
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 2 cut(s) 207, 444
AoxI GGCC 3 cut(s) 243, 534, 687
ApaI GGGCCC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 1069
ApoI RAATTY 1 cut(s) 827
ArsI GACNNNNNNTTYG 2 cut(s) 372, 404
AspS9I GGNCC 6 cut(s) 243, 244, 457, 535, 763, 796
AsuC2I CCSGG 1 cut(s) 761
AsuHPI GGTGA 2 cut(s) 592, 850
AvaI CYCGRG 2 cut(s) 207, 444
AvaII GGWCC 3 cut(s) 457, 763, 796
AxyI CCTNAGG 1 cut(s) 531
BaeGI GKGCMC 1 cut(s) 247
BalI TGGCCA 1 cut(s) 689
BanI GGYRCC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 247
BauI CACGAG 2 cut(s) 846, 876
BbsI GAAGAC 3 cut(s) 131, 312, 385
BbvI GCAGC 1 cut(s) 1056
BciT130I CCWGG 5 cut(s) 30, 185, 354, 539, 605
BclI TGATCA 2 cut(s) 397, 855
BcnI CCSGG 1 cut(s) 761
BcoDI GTCTC 3 cut(s) 778, 783, 804
BcuI ACTAGT 1 cut(s) 293
BfaI CTAG 1 cut(s) 294
BglII AGATCT 1 cut(s) 462
BisI GCNGC 1 cut(s) 1070
BlsI GCNGC 1 cut(s) 1071
Bme1390I CCNGG 6 cut(s) 30, 185, 354, 539, 605, 761
Bme18I GGWCC 3 cut(s) 457, 763, 796
BmeT110I CYCGRG 2 cut(s) 207, 444
BmgT120I GGNCC 6 cut(s) 243, 244, 457, 535, 763, 796
BmiI GGNNCC 3 cut(s) 201, 245, 764
BmrFI CCNGG 6 cut(s) 30, 185, 354, 539, 605, 761
BmrI ACTGGG 1 cut(s) 129
BmuI ACTGGG 1 cut(s) 129
BpiI GAAGAC 3 cut(s) 131, 312, 385
BplI GAGNNNNNCTC 2 cut(s) 29, 61
BpmI CTGGAG 2 cut(s) 336, 560
BpuMI CCSGG 1 cut(s) 761
BsaJI CCNNGG 6 cut(s) 184, 247, 537, 603, 684, 784
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 124
Bse21I CCTNAGG 1 cut(s) 531
BseBI CCWGG 5 cut(s) 30, 185, 354, 539, 605
BseDI CCNNGG 6 cut(s) 184, 247, 537, 603, 684, 784
BseGI GGATG 1 cut(s) 1018
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 522, 927
BseNI ACTGG 1 cut(s) 124
BseRI GAGGAG 3 cut(s) 659, 1079, 1082
BseSI GKGCMC 1 cut(s) 247
BseXI GCAGC 1 cut(s) 1056
BseYI CCCAGC 1 cut(s) 930
BshFI GGCC 3 cut(s) 245, 536, 689
BshNI GGYRCC 1 cut(s) 199
BsiHKCI CYCGRG 2 cut(s) 207, 444
BsiSI CCGG 1 cut(s) 760
BslFI GGGAC 2 cut(s) 56, 776
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 3 cut(s) 778, 783, 804
BsmFI GGGAC 2 cut(s) 56, 776
BsmI GAATGC 1 cut(s) 1029
BsnI GGCC 3 cut(s) 245, 536, 689
BsoBI CYCGRG 2 cut(s) 207, 444
Bsp120I GGGCCC 1 cut(s) 243
Bsp1286I GDGCHC 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 889
Bsp143I GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
Bsp19I CCATGG 1 cut(s) 784
BspANI GGCC 3 cut(s) 245, 536, 689
BspCNI CTCAG 2 cut(s) 523, 928
BspHI TCATGA 3 cut(s) 237, 558, 819
BspLI GGNNCC 3 cut(s) 201, 245, 764
BspT107I GGYRCC 1 cut(s) 199
BsrGI TGTACA 1 cut(s) 889
BsrI ACTGG 1 cut(s) 124
BssECI CCNNGG 6 cut(s) 184, 247, 537, 603, 684, 784
BssMI GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
BssSI CACGAG 2 cut(s) 846, 876
BssT1I CCWWGG 3 cut(s) 247, 684, 784
Bst2BI CACGAG 2 cut(s) 846, 876
Bst2UI CCWGG 5 cut(s) 30, 185, 354, 539, 605
Bst4CI ACNGT 2 cut(s) 384, 772
BstAUI TGTACA 1 cut(s) 889
BstC8I GCNNGC 3 cut(s) 105, 216, 276
BstDEI CTNAG 3 cut(s) 374, 531, 936
BstDSI CCRYGG 1 cut(s) 784
BstF5I GGATG 1 cut(s) 1018
BstKTI GATC 8 cut(s) 400, 465, 546, 673, 858, 868, 922, 1090
BstMAI GTCTC 3 cut(s) 778, 783, 804
BstMBI GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
BstMWI GCNNNNNNNGC 1 cut(s) 931
BstNI CCWGG 5 cut(s) 30, 185, 354, 539, 605
BstNSI RCATGY 2 cut(s) 278, 282
BstSCI CCNGG 6 cut(s) 28, 183, 352, 537, 603, 759
BstSLI GKGCMC 1 cut(s) 247
BstV1I GCAGC 1 cut(s) 1056
BstV2I GAAGAC 3 cut(s) 131, 312, 385
BstX2I RGATCY 1 cut(s) 462
BstXI CCANNNNNNTGG 1 cut(s) 956
BstYI RGATCY 1 cut(s) 462
Bsu36I CCTNAGG 1 cut(s) 531
BsuRI GGCC 3 cut(s) 245, 536, 689
BtgI CCRYGG 1 cut(s) 784
BtsCI GGATG 1 cut(s) 1018
Cac8I GCNNGC 3 cut(s) 105, 216, 276
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 3 cut(s) 237, 558, 819
Cfr13I GGNCC 6 cut(s) 243, 244, 457, 535, 763, 796
CseI GACGC 1 cut(s) 732
Csp6I GTAC 1 cut(s) 890
CviQI GTAC 1 cut(s) 890
DdeI CTNAG 3 cut(s) 374, 531, 936
DpnI GATC 8 cut(s) 399, 464, 545, 672, 857, 867, 921, 1089
DpnII GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
DraIII CACNNNGTG 1 cut(s) 666
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
EaeI YGGCCR 1 cut(s) 687
Eco130I CCWWGG 3 cut(s) 247, 684, 784
Eco24I GRGCYC 1 cut(s) 247
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 3 cut(s) 457, 763, 796
Eco81I CCTNAGG 1 cut(s) 531
Eco88I CYCGRG 2 cut(s) 207, 444
EcoO109I RGGNCCY 3 cut(s) 243, 244, 535
EcoRI GAATTC 1 cut(s) 827
EcoRII CCWGG 5 cut(s) 28, 183, 352, 537, 603
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 3 cut(s) 247, 684, 784
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 247
ErhI CCWWGG 3 cut(s) 247, 684, 784
FalI AAGNNNNNCTT 2 cut(s) 888, 920
FaqI GGGAC 2 cut(s) 56, 776
FbaI TGATCA 2 cut(s) 397, 855
Fnu4HI GCNGC 1 cut(s) 1070
FokI GGATG 1 cut(s) 1025
FriOI GRGCYC 1 cut(s) 247
Fsp4HI GCNGC 1 cut(s) 1070
FspBI CTAG 1 cut(s) 294
GluI GCNGC 1 cut(s) 1070
GsaI CCCAGC 1 cut(s) 934
GsuI CTGGAG 2 cut(s) 336, 560
HaeIII GGCC 3 cut(s) 245, 536, 689
HapII CCGG 1 cut(s) 760
HgaI GACGC 1 cut(s) 732
HincII GTYRAC 1 cut(s) 741
HindII GTYRAC 1 cut(s) 741
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 3 cut(s) 97, 496, 577
HpaII CCGG 1 cut(s) 760
HphI GGTGA 2 cut(s) 592, 850
Hpy166II GTNNAC 2 cut(s) 637, 741
Hpy188I TCNGA 1 cut(s) 1097
Hpy8I GTNNAC 2 cut(s) 637, 741
Hpy99I CGWCG 1 cut(s) 726
HpyAV CCTTC 5 cut(s) 145, 226, 602, 976, 1083
HpyCH4III ACNGT 2 cut(s) 384, 772
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 5 cut(s) 8, 274, 278, 779, 992
HpyF10VI GCNNNNNNNGC 1 cut(s) 931
HpyF3I CTNAG 3 cut(s) 374, 531, 936
HpySE526I ACGT 1 cut(s) 313
Ksp22I TGATCA 2 cut(s) 397, 855
Kzo9I GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
LmnI GCTCC 1 cut(s) 1066
Lsp1109I GCAGC 1 cut(s) 1056
MaeI CTAG 1 cut(s) 294
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 384, 1003
MalI GATC 8 cut(s) 399, 464, 545, 672, 857, 867, 921, 1089
MboI GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
MboII GAAGA 5 cut(s) 136, 143, 317, 390, 981
MflI RGATCY 1 cut(s) 462
MhlI GDGCHC 1 cut(s) 247
MlsI TGGCCA 1 cut(s) 689
MluCI AATT 4 cut(s) 66, 612, 827, 954
MluNI TGGCCA 1 cut(s) 689
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 9 cut(s) 55, 234, 418, 526, 567, 637, 816, 956, 1057
Mox20I TGGCCA 1 cut(s) 689
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 689
MseI TTAA 4 cut(s) 81, 267, 615, 912
MslI CAYNNNNRTG 2 cut(s) 279, 818
Msp20I TGGCCA 1 cut(s) 689
MspI CCGG 1 cut(s) 760
MspR9I CCNGG 6 cut(s) 30, 185, 354, 539, 605, 761
Mva1269I GAATGC 1 cut(s) 1029
MvaI CCWGG 5 cut(s) 30, 185, 354, 539, 605
MwoI GCNNNNNNNGC 1 cut(s) 931
NciI CCSGG 1 cut(s) 761
NcoI CCATGG 1 cut(s) 784
NdeII GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
NlaIV GGNNCC 3 cut(s) 201, 245, 764
NmuCI GTSAC 1 cut(s) 384
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 2 cut(s) 278, 282
PaeI GCATGC 1 cut(s) 278
PaeR7I CTCGAG 1 cut(s) 207
PagI TCATGA 3 cut(s) 237, 558, 819
PctI GAATGC 1 cut(s) 1029
PfeI GAWTC 3 cut(s) 97, 496, 577
PflMI CCANNNNNTGG 1 cut(s) 190
PkrI GCNGC 1 cut(s) 1071
Psp6I CCWGG 5 cut(s) 28, 183, 352, 537, 603
PspFI CCCAGC 1 cut(s) 930
PspGI CCWGG 5 cut(s) 28, 183, 352, 537, 603
PspN4I GGNNCC 3 cut(s) 201, 245, 764
PspOMI GGGCCC 1 cut(s) 243
PspPI GGNCC 6 cut(s) 243, 244, 457, 535, 763, 796
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 1 cut(s) 462
RsaI GTAC 1 cut(s) 891
RsaNI GTAC 1 cut(s) 890
RseI CAYNNNNRTG 2 cut(s) 279, 818
SaqAI TTAA 4 cut(s) 81, 267, 615, 912
SatI GCNGC 1 cut(s) 1070
Sau3AI GATC 8 cut(s) 397, 462, 543, 670, 855, 865, 919, 1087
Sau96I GGNCC 6 cut(s) 243, 244, 457, 535, 763, 796
ScrFI CCNGG 6 cut(s) 30, 185, 354, 539, 605, 761
SduI GDGCHC 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 207
SinI GGWCC 3 cut(s) 457, 763, 796
SlaI CTCGAG 1 cut(s) 207
SmiMI CAYNNNNRTG 2 cut(s) 279, 818
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
SpeI ACTAGT 1 cut(s) 293
SphI GCATGC 1 cut(s) 278
Sse9I AATT 4 cut(s) 66, 612, 827, 954
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 6 cut(s) 28, 183, 352, 537, 603, 759
StyI CCWWGG 3 cut(s) 247, 684, 784
TaaI ACNGT 2 cut(s) 384, 772
TaiI ACGT 1 cut(s) 316
TaqI TCGA 2 cut(s) 208, 721
TaqII GACCGA 1 cut(s) 758
TasI AATT 4 cut(s) 66, 612, 827, 954
TatI WGTACW 1 cut(s) 889
TfiI GAWTC 3 cut(s) 97, 496, 577
Tru1I TTAA 4 cut(s) 81, 267, 615, 912
Tru9I TTAA 4 cut(s) 81, 267, 615, 912
TseFI GTSAC 1 cut(s) 384
TseI GCWGC 1 cut(s) 1069
Tsp45I GTSAC 1 cut(s) 384
TspDTI ATGAA 6 cut(s) 323, 447, 547, 575, 609, 693
TspGWI ACGGA 1 cut(s) 821
Van91I CCANNNNNTGG 1 cut(s) 190
VpaK11BI GGWCC 3 cut(s) 457, 763, 796
XapI RAATTY 1 cut(s) 827
XceI RCATGY 2 cut(s) 278, 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XhoI CTCGAG 1 cut(s) 207
XspI CTAG 1 cut(s) 294
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.