Rh2DG458800

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000799
Physical Location & Seq
Reverse (-)
66336564 .. 66339604
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG458800.1

Sequence Viewer

Length: 1209 bp
ATGTCACTATTGATATTTTGCCTAATTTGTTGCAGCCTCTCATCTGAAGGTCAAGAGTTCTTCACATCGTATGATGAGGTTCATGACAGTTTTGATGCCATGGGTTTACAAGAAAATCTTTTAAGGGGAATATATGCTTATGGATTTGAGAAGCCTTCTGCGATTCAGCAAAGAGGAATTGTTCCTTTCTGTAAAGGTTTGGATGTGATTCAACAAGCTCAGTCAGGAACAGGCAAAACAGCAACCTTTTGCTCTGGAATTTTGCAGCAACTTGACTATGGTTTGGTCCAGTGCCAGGCTTTGGTGTTGGCACCTACCAGGGAGTTAGCTCAGCAGATTGAGAAGGTTATGCGGGCACTTGGAGACTATCTCGGCGTGAAGGTTCATGCTTGTGTAGGTGGGACTAGTGTTCGTGAGGATCAGCGAATTCTTCAGACTGGAGTGCATGTGGTGGTTGGCACTCCTGGACGTGTGTTTGACATGTTGCGGAGGCAATCTCTTCGTGCAGATTACATTAAGATGTTTGTATTGGATGAAGCCGATGAAATGCTATCTAGGGGTTTCAAAGATCAGATCTATGACATTTTTCAGCTTCTGCCTTCAAAAGTTCAAGTTGGTGTGTTCTCTGCTACAATGCCTCCTGAAGCCCTTGAGATCACCAGAAAGTTTATGAACAAACCTGTTAGGATTTTGGTTAAGCGTGATGAGCTCACCCTGGAGGGAATCAAGCAATTTTATGTTAATGTCGATAAGGAAGAATGGAAGCTGGAGACACTCTGCGATCTCTATGAGACCCTTGCAATCACTCAGAGTGTCATTTTTGTTAATACAAGGCGCAAAGTGGATTGGCTTACAGATAAGATGCGAAGCCGTGACCACACTGTGTCTGCTACCCATGGTGATATGGACCAGAACCAGCGTGACATTATCATGCGTGAATTCCGATCTGGTTCCTCCCGTGTTCTCATCACTACTGATCTGTTGGCACGTGGTATTGATGTGCAGCAAGTGTCGCTTGTAATTAATTATGATTTGCCGACTCAACCAGAAAACTACCTTCATCGTATCGGACGAAGTGGGCGATTTGGAAGAAAAGGTGTTGCCATCAATTTCGTGACTAGGGATGACGATAGAATGCTATATGACATTCAGAGGTTTTATAATGTGGTGATTGAGGAGCTGCCTTCCAACGTGGCTGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

402

Amino Acids

45.74

Weight (kDa)

5.8

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 54 - 216 1.6e-43 DEAD/DEAH box helicase
ResIII PF04851 72 - 212 7.6e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 255 - 363 2.8e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1161
AccB1I GGYRCC 1 cut(s) 310
AccB7I CCANNNNNTGG 1 cut(s) 301
AciI CCGC 2 cut(s) 352, 487
AclWI GGATC 1 cut(s) 426
AcsI RAATTY 3 cut(s) 258, 426, 938
AcuI CTGAAG 3 cut(s) 66, 416, 663
AcvI CACGTG 1 cut(s) 989
AdeI CACNNNGTG 1 cut(s) 883
AfiI CCNNNNNNNGG 2 cut(s) 295, 301
AflIII ACRYGT 2 cut(s) 469, 480
AgsI TTSAA 4 cut(s) 212, 565, 603, 611
AhlI ACTAGT 1 cut(s) 404
AjiI CACGTC 1 cut(s) 470
AjnI CCWGG 4 cut(s) 294, 317, 463, 714
AluBI AGCT 6 cut(s) 218, 329, 592, 709, 766, 1180
AluI AGCT 6 cut(s) 218, 329, 592, 709, 766, 1180
Alw21I GWGCWC 1 cut(s) 711
Alw26I GTCTC 3 cut(s) 357, 764, 785
AlwI GGATC 1 cut(s) 426
AlwNI CAGNNNCTG 1 cut(s) 595
ApeKI GCWGC 4 cut(s) 33, 265, 1003, 1180
ApoI RAATTY 3 cut(s) 258, 426, 938
AseI ATTAAT 1 cut(s) 1023
AspLEI GCGC 1 cut(s) 837
AspS9I GGNCC 2 cut(s) 286, 907
AsuHPI GGTGA 4 cut(s) 649, 703, 911, 1180
AvaII GGWCC 2 cut(s) 286, 907
BaeGI GKGCMC 1 cut(s) 358
BanI GGYRCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 711
BbrPI CACGTG 1 cut(s) 989
Bbv12I GWGCWC 1 cut(s) 711
BbvI GCAGC 4 cut(s) 45, 277, 1015, 1167
BccI CCATC 1 cut(s) 1112
BceAI ACGGC 1 cut(s) 855
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 4 cut(s) 296, 319, 465, 716
BcoDI GTCTC 3 cut(s) 357, 764, 785
BcuI ACTAGT 1 cut(s) 404
BfaI CTAG 3 cut(s) 405, 555, 1119
BglII AGATCT 1 cut(s) 573
BisI GCNGC 4 cut(s) 34, 266, 1004, 1181
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 4 cut(s) 35, 267, 1005, 1182
Bme1390I CCNGG 4 cut(s) 296, 319, 465, 716
Bme18I GGWCC 2 cut(s) 286, 907
BmgBI CACGTC 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 286, 907
BmiI GGNNCC 2 cut(s) 312, 952
BmrFI CCNGG 4 cut(s) 296, 319, 465, 716
BmsI GCATC 2 cut(s) 85, 852
BplI GAGNNNNNCTC 4 cut(s) 354, 386, 481, 513
BpmI CTGGAG 3 cut(s) 459, 737, 788
Bpu1102I GCTNAGC 1 cut(s) 330
BpuEI CTTGAG 1 cut(s) 671
BsaAI YACGTR 1 cut(s) 989
BsaI GGTCTC 1 cut(s) 785
BsaJI CCNNGG 4 cut(s) 99, 318, 714, 895
BsaXI ACNNNNNCTCC 4 cut(s) 432, 462, 622, 652
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 301
Bse1I ACTGG 2 cut(s) 289, 442
BseBI CCWGG 4 cut(s) 296, 319, 465, 716
BseDI CCNNGG 4 cut(s) 99, 318, 714, 895
BseGI GGATG 3 cut(s) 208, 538, 1129
BseLI CCNNNNNNNGG 2 cut(s) 295, 301
BseMII CTCAG 3 cut(s) 233, 344, 821
BseNI ACTGG 2 cut(s) 289, 442
BseRI GAGGAG 1 cut(s) 1190
BseSI GKGCMC 1 cut(s) 358
BseXI GCAGC 4 cut(s) 45, 277, 1015, 1167
BsgI GTGCAG 2 cut(s) 525, 1022
BshNI GGYRCC 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 711
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 2 cut(s) 295, 301
BsmAI GTCTC 3 cut(s) 357, 764, 785
BsmFI GGGAC 1 cut(s) 415
BsmI GAATGC 1 cut(s) 1140
Bso31I GGTCTC 1 cut(s) 785
Bsp1286I GDGCHC 2 cut(s) 358, 711
Bsp143I GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
Bsp1720I GCTNAGC 1 cut(s) 330
Bsp19I CCATGG 2 cut(s) 99, 895
BspACI CCGC 2 cut(s) 352, 487
BspCNI CTCAG 3 cut(s) 232, 343, 820
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 2 cut(s) 312, 952
BspPI GGATC 1 cut(s) 426
BspT107I GGYRCC 1 cut(s) 310
BspTNI GGTCTC 1 cut(s) 785
BsrI ACTGG 2 cut(s) 289, 442
BssECI CCNNGG 4 cut(s) 99, 318, 714, 895
BssMI GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
BssT1I CCWWGG 2 cut(s) 99, 895
Bst2UI CCWGG 4 cut(s) 296, 319, 465, 716
Bst4CI ACNGT 2 cut(s) 89, 883
Bst6I CTCTTC 1 cut(s) 504
BstBAI YACGTR 1 cut(s) 989
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 3 cut(s) 219, 330, 807
BstDSI CCRYGG 2 cut(s) 99, 895
BstF5I GGATG 3 cut(s) 208, 538, 1129
BstHHI GCGC 1 cut(s) 837
BstKTI GATC 8 cut(s) 421, 571, 576, 657, 784, 947, 979, 1201
BstMAI GTCTC 3 cut(s) 357, 764, 785
BstMBI GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
BstMWI GCNNNNNNNGC 2 cut(s) 706, 1012
BstNI CCWGG 4 cut(s) 296, 319, 465, 716
BstNSI RCATGY 2 cut(s) 449, 484
BstSCI CCNGG 4 cut(s) 294, 317, 463, 714
BstSLI GKGCMC 1 cut(s) 358
BstV1I GCAGC 4 cut(s) 45, 277, 1015, 1167
BstX2I RGATCY 1 cut(s) 573
BstYI RGATCY 1 cut(s) 573
BtgI CCRYGG 2 cut(s) 99, 895
BtrI CACGTC 1 cut(s) 470
BtsCI GGATG 3 cut(s) 208, 538, 1129
BtsIMutI CAGTG 2 cut(s) 296, 879
Cac8I GCNNGC 1 cut(s) 354
CaiI CAGNNNCTG 1 cut(s) 595
CciI TCATGA 1 cut(s) 82
CfoI GCGC 1 cut(s) 837
Cfr13I GGNCC 2 cut(s) 286, 907
CviAII CATG 7 cut(s) 83, 100, 386, 446, 481, 896, 931
DdeI CTNAG 3 cut(s) 219, 330, 807
DpnI GATC 8 cut(s) 420, 570, 575, 656, 783, 946, 978, 1200
DpnII GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
DraIII CACNNNGTG 1 cut(s) 883
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Ecl136II GAGCTC 1 cut(s) 709
Eco130I CCWWGG 2 cut(s) 99, 895
Eco24I GRGCYC 1 cut(s) 711
Eco31I GGTCTC 1 cut(s) 785
Eco47I GGWCC 2 cut(s) 286, 907
Eco53kI GAGCTC 1 cut(s) 709
Eco57I CTGAAG 3 cut(s) 66, 416, 663
Eco72I CACGTG 1 cut(s) 989
EcoICRI GAGCTC 1 cut(s) 709
EcoRI GAATTC 2 cut(s) 426, 938
EcoRII CCWGG 4 cut(s) 294, 317, 463, 714
EcoT14I CCWWGG 2 cut(s) 99, 895
EcoT38I GRGCYC 1 cut(s) 711
ErhI CCWWGG 2 cut(s) 99, 895
FaeI CATG 7 cut(s) 86, 103, 389, 449, 484, 899, 934
FalI AAGNNNNNCTT 4 cut(s) 102, 134, 999, 1031
FaqI GGGAC 1 cut(s) 415
FatI CATG 7 cut(s) 82, 99, 385, 445, 480, 895, 930
FauI CCCGC 1 cut(s) 345
Fnu4HI GCNGC 4 cut(s) 34, 266, 1004, 1181
FokI GGATG 3 cut(s) 215, 545, 1136
FriOI GRGCYC 1 cut(s) 711
Fsp4HI GCNGC 4 cut(s) 34, 266, 1004, 1181
FspBI CTAG 3 cut(s) 405, 555, 1119
GlaI GCGC 1 cut(s) 836
GluI GCNGC 4 cut(s) 34, 266, 1004, 1181
GsuI CTGGAG 3 cut(s) 459, 737, 788
HhaI GCGC 1 cut(s) 837
Hin1II CATG 7 cut(s) 86, 103, 389, 449, 484, 899, 934
Hin6I GCGC 1 cut(s) 835
HinP1I GCGC 1 cut(s) 835
HinfI GANTC 4 cut(s) 163, 208, 723, 1039
HphI GGTGA 4 cut(s) 649, 703, 911, 1180
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 7 cut(s) 46, 435, 573, 810, 944, 1070, 1152
Hpy188III TCNNGA 7 cut(s) 53, 83, 225, 255, 413, 641, 1114
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 7 cut(s) 41, 165, 337, 373, 609, 1067, 1194
HpyCH4III ACNGT 2 cut(s) 89, 883
HpyCH4IV ACGT 3 cut(s) 469, 988, 1191
HpyCH4V TGCA 6 cut(s) 33, 265, 445, 506, 800, 1003
HpyF10VI GCNNNNNNNGC 2 cut(s) 706, 1012
HpyF3I CTNAG 3 cut(s) 219, 330, 807
HpySE526I ACGT 3 cut(s) 469, 988, 1191
Hsp92II CATG 7 cut(s) 86, 103, 389, 449, 484, 899, 934
HspAI GCGC 1 cut(s) 835
Kzo9I GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
LmnI GCTCC 1 cut(s) 1177
Lsp1109I GCAGC 4 cut(s) 45, 277, 1015, 1167
LweI GCATC 2 cut(s) 85, 852
MaeI CTAG 3 cut(s) 405, 555, 1119
MaeII ACGT 3 cut(s) 469, 988, 1191
MaeIII GTNAC 4 cut(s) 3, 872, 920, 1114
MalI GATC 8 cut(s) 420, 570, 575, 656, 783, 946, 978, 1200
MboI GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
MboII GAAGA 6 cut(s) 52, 422, 491, 767, 1101, 1193
MflI RGATCY 1 cut(s) 573
MhlI GDGCHC 2 cut(s) 358, 711
MluCI AATT 9 cut(s) 24, 177, 258, 426, 731, 938, 1020, 1024, 1108
MlyI GAGTC 1 cut(s) 1033
MseI TTAA 6 cut(s) 122, 516, 696, 741, 825, 1023
MslI CAYNNNNRTG 3 cut(s) 390, 518, 929
MspR9I CCNGG 4 cut(s) 296, 319, 465, 716
Mva1269I GAATGC 1 cut(s) 1140
MvaI CCWGG 4 cut(s) 296, 319, 465, 716
MwoI GCNNNNNNNGC 2 cut(s) 706, 1012
NcoI CCATGG 2 cut(s) 99, 895
NdeII GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
NlaIII CATG 7 cut(s) 86, 103, 389, 449, 484, 899, 934
NlaIV GGNNCC 2 cut(s) 312, 952
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 4 cut(s) 3, 872, 920, 1114
NspI RCATGY 2 cut(s) 449, 484
PagI TCATGA 1 cut(s) 82
PciI ACATGT 1 cut(s) 480
PcsI WCGNNNNNNNCGW 2 cut(s) 1069, 1078
PctI GAATGC 1 cut(s) 1140
PfeI GAWTC 3 cut(s) 163, 208, 723
PflMI CCANNNNNTGG 1 cut(s) 301
PfoI TCCNGGA 1 cut(s) 463
PkrI GCNGC 4 cut(s) 35, 267, 1005, 1182
PleI GAGTC 1 cut(s) 1033
PmaCI CACGTG 1 cut(s) 989
PmlI CACGTG 1 cut(s) 989
PpsI GAGTC 1 cut(s) 1033
Ppu21I YACGTR 1 cut(s) 989
PscI ACATGT 1 cut(s) 480
PshBI ATTAAT 1 cut(s) 1023
PsiI TTATAA 1 cut(s) 1161
Psp124BI GAGCTC 1 cut(s) 711
Psp6I CCWGG 4 cut(s) 294, 317, 463, 714
PspCI CACGTG 1 cut(s) 989
PspGI CCWGG 4 cut(s) 294, 317, 463, 714
PspN4I GGNNCC 2 cut(s) 312, 952
PspPI GGNCC 2 cut(s) 286, 907
PstNI CAGNNNCTG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 573
RseI CAYNNNNRTG 3 cut(s) 390, 518, 929
SacI GAGCTC 1 cut(s) 711
SaqAI TTAA 6 cut(s) 122, 516, 696, 741, 825, 1023
SatI GCNGC 4 cut(s) 34, 266, 1004, 1181
Sau3AI GATC 8 cut(s) 418, 568, 573, 654, 781, 944, 976, 1198
Sau96I GGNCC 2 cut(s) 286, 907
SchI GAGTC 1 cut(s) 1033
ScrFI CCNGG 4 cut(s) 296, 319, 465, 716
SduI GDGCHC 2 cut(s) 358, 711
SfaNI GCATC 2 cut(s) 85, 852
SinI GGWCC 2 cut(s) 286, 907
SmiMI CAYNNNNRTG 3 cut(s) 390, 518, 929
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
SpeI ACTAGT 1 cut(s) 404
Sse9I AATT 9 cut(s) 24, 177, 258, 426, 731, 938, 1020, 1024, 1108
SsiI CCGC 2 cut(s) 352, 487
SspMI CTAG 3 cut(s) 405, 555, 1119
SstI GAGCTC 1 cut(s) 711
StyD4I CCNGG 4 cut(s) 294, 317, 463, 714
StyI CCWWGG 2 cut(s) 99, 895
TaaI ACNGT 2 cut(s) 89, 883
TaiI ACGT 3 cut(s) 472, 991, 1194
TaqI TCGA 1 cut(s) 747
TasI AATT 9 cut(s) 24, 177, 258, 426, 731, 938, 1020, 1024, 1108
TfiI GAWTC 3 cut(s) 163, 208, 723
Tru1I TTAA 6 cut(s) 122, 516, 696, 741, 825, 1023
Tru9I TTAA 6 cut(s) 122, 516, 696, 741, 825, 1023
TscAI CASTG 2 cut(s) 296, 886
TseFI GTSAC 4 cut(s) 3, 872, 920, 1114
TseI GCWGC 4 cut(s) 33, 265, 1003, 1180
Tsp45I GTSAC 4 cut(s) 3, 872, 920, 1114
TspDTI ATGAA 6 cut(s) 71, 374, 549, 558, 686, 1049
TspRI CASTG 2 cut(s) 296, 886
Van91I CCANNNNNTGG 1 cut(s) 301
VpaK11BI GGWCC 2 cut(s) 286, 907
VspI ATTAAT 1 cut(s) 1023
XapI RAATTY 3 cut(s) 258, 426, 938
XceI RCATGY 2 cut(s) 449, 484
XspI CTAG 3 cut(s) 405, 555, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.