Rw2G035880

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000799
Physical Location & Seq
Reverse (-)
58972743 .. 58974637
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G035880.1

Sequence Viewer

Length: 1293 bp
ATGAGGTATCTACTGAATCTTAAAGTAGCAAATGTTTTCCTGTTCACAGATATGGCAGGTCTTGCTCCCGAAGGTGCACAGTTTGATGCTAAACAGTATGATTCTAAGATGAATGAGATCCTCTCATCTGAAGGCCAAGAGTTCTTCACATCGTATGATGAGGTTCATGACAGTTTTGATGCCATGGGTTTACAAGAAAATCTTTTAAGGGGAATATATGCTTATGGATTTGAGAAGCCTTCTGCGATTCAGCAAAGAGGAATTGTTCCTTTCTGTAAAGGTTTGGATGTGATTCAACAAGCTCAGTCAGGAACAGGCAAAACAGCAACCTTTTGCTCTGGAATTTTGCAGCAACTTGACTATGGTTTGGTCCAGTGCCAGGCTTTGGTGTTGGCACCTACCAGGGAGTTAGCTCAGCAGATTGAGAAGGTTATGCGGGCACTTGGAGACTATCTCGGCGTGAAGGTTCATGCTTGTGTAGGTGGGACTAGTGTTCGTGAGGATCAGCGAATTCTTCAGACTGGAGTGCATGTGGTGGTTGGCACTCCTGGACGTGTGTTTGACATGTTGCGGAGGCAATCTCTTCGTGCAGATTACATTAAGATGTTTGTATTGGATGAAGCCGATGAAATGCTATCTAGGGGTTTCAAAGATCAGATCTATGACATTTTTCAGCTTCTGCCTTCAAAAGTTCAAGTTGGTGTGTTCTCTGCTACAATGCCTCCTGAAGCCCTTGAGATCACCAGAAAGTTTATGAACAAACCTGTTAGGATTTTGGTTAAGCGTGATGAGCTCACCCTGGAGGGAATCAAGCAATTTTATGTTAATGTCGATAAGGAAGAATGGAAGCTGGAGACACTCTGCGATCTCTATGAGACCCTTGCAATCACTCAGAGTGTCATTTTTGTTAATACAAGGCGCAAAGTGGATTGGCTTACAGATAAGATGCGAAGCCGTGACCACACTGTGTCTGCTACCCATGGTGATATGGACCAGAACCAGCGTGACATTATCATGCGTGAATTCCGATCTGGTTCCTCCCGTGTTCTCATCACTACTGATCTGTTGGCACGTGGTATTGATGTGCAGCAAGTGTCGCTTGTAATTAATTATGATTTGCCGACTCAACCAGAAAACTACCTTCATCGTATCGGACGAAGTGGGCGATTTGGAAGAAAAGGTGTTGCCATCAATTTCGTGACTAGGGATGACGATAGAATGCTATATGACATTCAGAGGTTTTATAATGTGGTGATTGAGGAGCTGCCTTCCAACGTGGCTGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

430

Amino Acids

48.95

Weight (kDa)

5.69

Isoelectric Point (pI)

43.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 82 - 244 1.8e-43 DEAD/DEAH box helicase
ResIII PF04851 100 - 240 8.8e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 283 - 391 3.1e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1245
Acc36I ACCTGC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 394
AccB7I CCANNNNNTGG 1 cut(s) 385
AciI CCGC 2 cut(s) 436, 571
AclWI GGATC 2 cut(s) 112, 510
AcsI RAATTY 3 cut(s) 342, 510, 1022
AcuI CTGAAG 3 cut(s) 150, 500, 747
AcvI CACGTG 1 cut(s) 1073
AdeI CACNNNGTG 1 cut(s) 967
AfiI CCNNNNNNNGG 2 cut(s) 379, 385
AflIII ACRYGT 2 cut(s) 553, 564
AgsI TTSAA 4 cut(s) 296, 649, 687, 695
AhlI ACTAGT 1 cut(s) 488
AjiI CACGTC 1 cut(s) 554
AjnI CCWGG 4 cut(s) 378, 401, 547, 798
AluBI AGCT 6 cut(s) 302, 413, 676, 793, 850, 1264
AluI AGCT 6 cut(s) 302, 413, 676, 793, 850, 1264
Alw21I GWGCWC 2 cut(s) 79, 795
Alw26I GTCTC 3 cut(s) 441, 848, 869
Alw44I GTGCAC 1 cut(s) 75
AlwI GGATC 2 cut(s) 112, 510
AlwNI CAGNNNCTG 1 cut(s) 679
AoxI GGCC 1 cut(s) 133
ApaLI GTGCAC 1 cut(s) 75
ApeKI GCWGC 3 cut(s) 349, 1087, 1264
ApoI RAATTY 3 cut(s) 342, 510, 1022
AseI ATTAAT 1 cut(s) 1107
AspLEI GCGC 1 cut(s) 921
AspS9I GGNCC 2 cut(s) 370, 991
AsuHPI GGTGA 4 cut(s) 733, 787, 995, 1264
AvaII GGWCC 2 cut(s) 370, 991
BaeGI GKGCMC 2 cut(s) 79, 442
BanI GGYRCC 1 cut(s) 394
BanII GRGCYC 1 cut(s) 795
BbrPI CACGTG 1 cut(s) 1073
Bbv12I GWGCWC 2 cut(s) 79, 795
BbvI GCAGC 3 cut(s) 361, 1099, 1251
BccI CCATC 1 cut(s) 1196
BceAI ACGGC 1 cut(s) 939
BcgI CGANNNNNNTGC 2 cut(s) 566, 600
BciT130I CCWGG 4 cut(s) 380, 403, 549, 800
BcoDI GTCTC 3 cut(s) 441, 848, 869
BcuI ACTAGT 1 cut(s) 488
BfaI CTAG 3 cut(s) 489, 639, 1203
BfuAI ACCTGC 1 cut(s) 47
BglII AGATCT 1 cut(s) 657
BisI GCNGC 3 cut(s) 350, 1088, 1265
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 3 cut(s) 351, 1089, 1266
Bme1390I CCNGG 4 cut(s) 380, 403, 549, 800
Bme18I GGWCC 2 cut(s) 370, 991
BmgBI CACGTC 1 cut(s) 554
BmgT120I GGNCC 2 cut(s) 370, 991
BmiI GGNNCC 2 cut(s) 396, 1036
BmrFI CCNGG 4 cut(s) 380, 403, 549, 800
BmsI GCATC 3 cut(s) 76, 169, 936
BplI GAGNNNNNCTC 6 cut(s) 107, 139, 438, 470, 565, 597
BpmI CTGGAG 3 cut(s) 543, 821, 872
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 755
BsaAI YACGTR 1 cut(s) 1073
BsaI GGTCTC 1 cut(s) 869
BsaJI CCNNGG 4 cut(s) 183, 402, 798, 979
BsaXI ACNNNNNCTCC 4 cut(s) 516, 546, 706, 736
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 385
Bse1I ACTGG 2 cut(s) 373, 526
BseBI CCWGG 4 cut(s) 380, 403, 549, 800
BseDI CCNNGG 4 cut(s) 183, 402, 798, 979
BseGI GGATG 3 cut(s) 292, 622, 1213
BseLI CCNNNNNNNGG 2 cut(s) 379, 385
BseMII CTCAG 3 cut(s) 317, 428, 905
BseNI ACTGG 2 cut(s) 373, 526
BseRI GAGGAG 1 cut(s) 1274
BseSI GKGCMC 2 cut(s) 79, 442
BseXI GCAGC 3 cut(s) 361, 1099, 1251
BsgI GTGCAG 2 cut(s) 609, 1106
BshFI GGCC 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 394
BsiHKAI GWGCWC 2 cut(s) 79, 795
BslFI GGGAC 1 cut(s) 499
BslI CCNNNNNNNGG 2 cut(s) 379, 385
BsmAI GTCTC 3 cut(s) 441, 848, 869
BsmFI GGGAC 1 cut(s) 499
BsmI GAATGC 1 cut(s) 1224
BsnI GGCC 1 cut(s) 135
Bso31I GGTCTC 1 cut(s) 869
Bsp1286I GDGCHC 3 cut(s) 79, 442, 795
Bsp143I GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
Bsp1720I GCTNAGC 1 cut(s) 414
Bsp19I CCATGG 2 cut(s) 183, 979
BspACI CCGC 2 cut(s) 436, 571
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 3 cut(s) 316, 427, 904
BspHI TCATGA 1 cut(s) 166
BspLI GGNNCC 2 cut(s) 396, 1036
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 2 cut(s) 112, 510
BspT107I GGYRCC 1 cut(s) 394
BspTNI GGTCTC 1 cut(s) 869
BsrI ACTGG 2 cut(s) 373, 526
BssECI CCNNGG 4 cut(s) 183, 402, 798, 979
BssMI GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
BssT1I CCWWGG 2 cut(s) 183, 979
Bst2UI CCWGG 4 cut(s) 380, 403, 549, 800
Bst4CI ACNGT 4 cut(s) 81, 96, 173, 967
Bst6I CTCTTC 1 cut(s) 588
BstAPI GCANNNNNTGC 1 cut(s) 62
BstBAI YACGTR 1 cut(s) 1073
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 4 cut(s) 105, 303, 414, 891
BstDSI CCRYGG 2 cut(s) 183, 979
BstF5I GGATG 3 cut(s) 292, 622, 1213
BstHHI GCGC 1 cut(s) 921
BstKTI GATC 9 cut(s) 120, 505, 655, 660, 741, 868, 1031, 1063, 1285
BstMAI GTCTC 3 cut(s) 441, 848, 869
BstMBI GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
BstMWI GCNNNNNNNGC 3 cut(s) 62, 790, 1096
BstNI CCWGG 4 cut(s) 380, 403, 549, 800
BstNSI RCATGY 2 cut(s) 533, 568
BstSCI CCNGG 4 cut(s) 378, 401, 547, 798
BstSLI GKGCMC 2 cut(s) 79, 442
BstV1I GCAGC 3 cut(s) 361, 1099, 1251
BstX2I RGATCY 2 cut(s) 117, 657
BstYI RGATCY 2 cut(s) 117, 657
BsuRI GGCC 1 cut(s) 135
BtgI CCRYGG 2 cut(s) 183, 979
BtrI CACGTC 1 cut(s) 554
BtsCI GGATG 3 cut(s) 292, 622, 1213
BtsIMutI CAGTG 2 cut(s) 380, 963
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 438
CaiI CAGNNNCTG 1 cut(s) 679
CciI TCATGA 1 cut(s) 166
CfoI GCGC 1 cut(s) 921
Cfr13I GGNCC 2 cut(s) 370, 991
CviAII CATG 7 cut(s) 167, 184, 470, 530, 565, 980, 1015
DdeI CTNAG 4 cut(s) 105, 303, 414, 891
DpnI GATC 9 cut(s) 119, 504, 654, 659, 740, 867, 1030, 1062, 1284
DpnII GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
DraIII CACNNNGTG 1 cut(s) 967
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Ecl136II GAGCTC 1 cut(s) 793
Eco130I CCWWGG 2 cut(s) 183, 979
Eco24I GRGCYC 1 cut(s) 795
Eco31I GGTCTC 1 cut(s) 869
Eco47I GGWCC 2 cut(s) 370, 991
Eco53kI GAGCTC 1 cut(s) 793
Eco57I CTGAAG 3 cut(s) 150, 500, 747
Eco72I CACGTG 1 cut(s) 1073
EcoICRI GAGCTC 1 cut(s) 793
EcoRI GAATTC 2 cut(s) 510, 1022
EcoRII CCWGG 4 cut(s) 378, 401, 547, 798
EcoT14I CCWWGG 2 cut(s) 183, 979
EcoT38I GRGCYC 1 cut(s) 795
ErhI CCWWGG 2 cut(s) 183, 979
FaeI CATG 7 cut(s) 170, 187, 473, 533, 568, 983, 1018
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 1083, 1115
FaqI GGGAC 1 cut(s) 499
FatI CATG 7 cut(s) 166, 183, 469, 529, 564, 979, 1014
FauI CCCGC 1 cut(s) 429
Fnu4HI GCNGC 3 cut(s) 350, 1088, 1265
FokI GGATG 3 cut(s) 299, 629, 1220
FriOI GRGCYC 1 cut(s) 795
Fsp4HI GCNGC 3 cut(s) 350, 1088, 1265
FspBI CTAG 3 cut(s) 489, 639, 1203
GlaI GCGC 1 cut(s) 920
GluI GCNGC 3 cut(s) 350, 1088, 1265
GsuI CTGGAG 3 cut(s) 543, 821, 872
HaeIII GGCC 1 cut(s) 135
HhaI GCGC 1 cut(s) 921
Hin1II CATG 7 cut(s) 170, 187, 473, 533, 568, 983, 1018
Hin6I GCGC 1 cut(s) 919
HinP1I GCGC 1 cut(s) 919
HinfI GANTC 6 cut(s) 16, 101, 247, 292, 807, 1123
HphI GGTGA 4 cut(s) 733, 787, 995, 1264
Hpy166II GTNNAC 3 cut(s) 45, 77, 191
Hpy188I TCNGA 7 cut(s) 130, 519, 657, 894, 1028, 1154, 1236
Hpy188III TCNNGA 7 cut(s) 68, 167, 309, 339, 497, 725, 1198
Hpy8I GTNNAC 3 cut(s) 45, 77, 191
HpyAV CCTTC 8 cut(s) 65, 125, 249, 421, 457, 693, 1151, 1278
HpyCH4III ACNGT 4 cut(s) 81, 96, 173, 967
HpyCH4IV ACGT 3 cut(s) 553, 1072, 1275
HpyCH4V TGCA 6 cut(s) 77, 349, 529, 590, 884, 1087
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 790, 1096
HpyF3I CTNAG 4 cut(s) 105, 303, 414, 891
HpySE526I ACGT 3 cut(s) 553, 1072, 1275
Hsp92II CATG 7 cut(s) 170, 187, 473, 533, 568, 983, 1018
HspAI GCGC 1 cut(s) 919
Kzo9I GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
LmnI GCTCC 2 cut(s) 70, 1261
Lsp1109I GCAGC 3 cut(s) 361, 1099, 1251
LweI GCATC 3 cut(s) 76, 169, 936
MaeI CTAG 3 cut(s) 489, 639, 1203
MaeII ACGT 3 cut(s) 553, 1072, 1275
MaeIII GTNAC 3 cut(s) 956, 1004, 1198
MalI GATC 9 cut(s) 119, 504, 654, 659, 740, 867, 1030, 1062, 1284
MboI GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
MboII GAAGA 6 cut(s) 136, 506, 575, 851, 1185, 1277
MflI RGATCY 2 cut(s) 117, 657
MhlI GDGCHC 3 cut(s) 79, 442, 795
MluCI AATT 8 cut(s) 261, 342, 510, 815, 1022, 1104, 1108, 1192
MlyI GAGTC 1 cut(s) 1117
MseI TTAA 7 cut(s) 21, 206, 600, 780, 825, 909, 1107
MslI CAYNNNNRTG 4 cut(s) 50, 474, 602, 1013
MspR9I CCNGG 4 cut(s) 380, 403, 549, 800
Mva1269I GAATGC 1 cut(s) 1224
MvaI CCWGG 4 cut(s) 380, 403, 549, 800
MwoI GCNNNNNNNGC 3 cut(s) 62, 790, 1096
NcoI CCATGG 2 cut(s) 183, 979
NdeII GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
NlaIII CATG 7 cut(s) 170, 187, 473, 533, 568, 983, 1018
NlaIV GGNNCC 2 cut(s) 396, 1036
NmeAIII GCCGAG 1 cut(s) 435
NmuCI GTSAC 3 cut(s) 956, 1004, 1198
NspI RCATGY 2 cut(s) 533, 568
PagI TCATGA 1 cut(s) 166
PciI ACATGT 1 cut(s) 564
PcsI WCGNNNNNNNCGW 2 cut(s) 1153, 1162
PctI GAATGC 1 cut(s) 1224
PfeI GAWTC 5 cut(s) 16, 101, 247, 292, 807
PflMI CCANNNNNTGG 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 547
PkrI GCNGC 3 cut(s) 351, 1089, 1266
PleI GAGTC 1 cut(s) 1117
PmaCI CACGTG 1 cut(s) 1073
PmlI CACGTG 1 cut(s) 1073
PpsI GAGTC 1 cut(s) 1117
Ppu21I YACGTR 1 cut(s) 1073
PscI ACATGT 1 cut(s) 564
PshBI ATTAAT 1 cut(s) 1107
PsiI TTATAA 1 cut(s) 1245
Psp124BI GAGCTC 1 cut(s) 795
Psp6I CCWGG 4 cut(s) 378, 401, 547, 798
PspCI CACGTG 1 cut(s) 1073
PspGI CCWGG 4 cut(s) 378, 401, 547, 798
PspN4I GGNNCC 2 cut(s) 396, 1036
PspPI GGNCC 2 cut(s) 370, 991
PstNI CAGNNNCTG 1 cut(s) 679
PsuI RGATCY 2 cut(s) 117, 657
RseI CAYNNNNRTG 4 cut(s) 50, 474, 602, 1013
SacI GAGCTC 1 cut(s) 795
SaqAI TTAA 7 cut(s) 21, 206, 600, 780, 825, 909, 1107
SatI GCNGC 3 cut(s) 350, 1088, 1265
Sau3AI GATC 9 cut(s) 117, 502, 652, 657, 738, 865, 1028, 1060, 1282
Sau96I GGNCC 2 cut(s) 370, 991
SchI GAGTC 1 cut(s) 1117
ScrFI CCNGG 4 cut(s) 380, 403, 549, 800
SduI GDGCHC 3 cut(s) 79, 442, 795
SfaNI GCATC 3 cut(s) 76, 169, 936
SinI GGWCC 2 cut(s) 370, 991
SmiMI CAYNNNNRTG 4 cut(s) 50, 474, 602, 1013
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
SpeI ACTAGT 1 cut(s) 488
Sse9I AATT 8 cut(s) 261, 342, 510, 815, 1022, 1104, 1108, 1192
SsiI CCGC 2 cut(s) 436, 571
SspMI CTAG 3 cut(s) 489, 639, 1203
SstI GAGCTC 1 cut(s) 795
StyD4I CCNGG 4 cut(s) 378, 401, 547, 798
StyI CCWWGG 2 cut(s) 183, 979
TaaI ACNGT 4 cut(s) 81, 96, 173, 967
TaiI ACGT 3 cut(s) 556, 1075, 1278
TaqI TCGA 1 cut(s) 831
TasI AATT 8 cut(s) 261, 342, 510, 815, 1022, 1104, 1108, 1192
TfiI GAWTC 5 cut(s) 16, 101, 247, 292, 807
Tru1I TTAA 7 cut(s) 21, 206, 600, 780, 825, 909, 1107
Tru9I TTAA 7 cut(s) 21, 206, 600, 780, 825, 909, 1107
TscAI CASTG 2 cut(s) 380, 970
TseFI GTSAC 3 cut(s) 956, 1004, 1198
TseI GCWGC 3 cut(s) 349, 1087, 1264
Tsp45I GTSAC 3 cut(s) 956, 1004, 1198
TspDTI ATGAA 7 cut(s) 125, 155, 458, 633, 642, 770, 1133
TspRI CASTG 2 cut(s) 380, 970
Van91I CCANNNNNTGG 1 cut(s) 385
VneI GTGCAC 1 cut(s) 75
VpaK11BI GGWCC 2 cut(s) 370, 991
VspI ATTAAT 1 cut(s) 1107
XapI RAATTY 3 cut(s) 342, 510, 1022
XceI RCATGY 2 cut(s) 533, 568
XspI CTAG 3 cut(s) 489, 639, 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.