RLG00000016437

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000799
Physical Location & Seq
Reverse (-)
6639881 .. 6646180
6300 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016437

Sequence Viewer

Length: 2118 bp
ATGTACGGCGACGCAAGGAACTGGGACGAAGAAGCCTACAGGGAAACCATTCTAAAGGAGCGAGAGATTCAAACCCGAACCGTTTTCCGAACCGCCTGGGCTCCCTCCCTTAACCCTAACCCCGACACCATCGTCGTCGCCTCCAGCGATGGATCCGTTTCGTCCTACTCCATCCCCTCCCTCGTCTCCAAGCTCCCACTAGGTTTCAGCAATGTGAAAGCTCCATACTTGTTAATGGCGGAACCTAATTGCTTCCTTAAAGCGCATGAGGGGCCTGCATATGACGTTAAATTTTATGGCCACGGGGGCGAGGAGTCTATGCTTCTCAGGGGTCCTTGGGGGGCTTTATCTCCAATTCCTGAAAACAATGCTCTTGCTGTTGATAATCAAGGAGGGTCCATTTTCTCTGCTGCTGGCGATTCTTGTGTCTATTGCTGGGATGTGGAAACAGGTCAAGTCAAAATGATTTTTAAGGGGCACTCGGATTACTTGCATTCCATAGTCGCCCGAAACTCTACAAATCAGATCATAACTGGTTCAGAGGATGGGACGGCACGTATATGGGATTGCAAAAGTGGAAAGTCAGTTCAAGTGATTGAGCCATTAAAGAATAATAAATTGAAAGGGTCCAGAGTAGCTTTCTTCCATATTTCTCTAAAAGAAAATTCTATATTAGCAGTTGGAGCAGAGCCTCTACTTAGCCGTTTTGACATAAATGGGGTGATTCTTTCACAAATGCAATGTGCCCCTCAGTCAGCCTTTTCTGTCTCATTACACGCATCTGGGGTCACAGCAATAGGAGGTTATGGAGGAGTTGTGGATTTCGATTTCCTTGTCCTTCTTTTTCACTCTTCGAACAAATCAACATCATCATCCACAGTCATGGCGGCACCTGAAGGTTCACAGTTTGATACGCGTCAGTTCGACACCAAGATGAATGAGTTGCTTGCAACTGATGGACAAGATTTCTTTACATCATACGATGAAGTTTACGACAGTTTTGATTCCATGGGCTTGCAAGAGAACCTGCTGAGGGGCATTTATGCTTACGGTTTTGAGAAACCCTCTGCCATTCAGCAAAGGGGAATTGTCCCCTTTATTAAGGGTCTTGATGTGATTCAGCAGGCTCAATCTGGAACTGGGAAGACAGCGACCTTCTGTTCTGGTATTCTTCAACAGCTTGAGTATAGTTTGATGGAGTGCCAGGGCTTGGTTCTGGCACCCACTCGAGAGCTTGCCCAACAAATAGAGAAGGTCATGAGGGCCCTTGGAGATTATCTTGGTGTTAAGGTGCATGCATGTGTTGGTGGAACTAGTGTTCGTGAAGACCAACGTATTCTGTCAAATGGAGTTCATGCTGTTGTTGGAACTCCAGGTCGTGTTTTTGATATGCTAAGAAGACAGTCACTTCGCCCTGATCATATCAAGATGTTTGTCCTTGATGAGGCTGATGAAATGCTCTCTCGGGGTTTCAAGGACCAGATCTATGATATATTCCAGCTTCTCCCACCAAAGATTCAGGTTGGTGTGTTTTCTGCCACCATGCCACCTGAGGCCCTGGAGATCACAAGGAAGTTCATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAACTCACCCTGGAAGGAATTAAGCAGTTTTATGTCAATGTGGACAAGGAGGAGTGGAAGCTTGACACTTTGTGTGATCTTTATGAAACCTTGGCCATTACCCAGAGTGTGATTTTCGTGAATACTCGACGCAAGGTTGATTGGTTGACCGACAAAATGAGAAGCCGGGACCATACAGTCTCTGCAACCCATGGAGACATGGACCAGAACAACAGAGACATCATCATGAGGGAATTCCGTTCTGGGTCATCTCGTGTCTTGATCACCACCGATCTGTTGGCTCGTGGTATTGATGTACAACAAGTTTCCCTTGTTATTAACTATGATCTGCCTACCCAGCCTGAGAACTACCTCCATAGAATTGGTCGTAGTGGAAGATTTGGTAGGAAGGGAGTTGCAATCAACTTTGTTACAAAGGATGATGAGAGAATGCTGTTTGATATCCAGAAGTTCTACAATGTGGTAGTAGAGGAGCTGCCTTCAAATGTTGCTGATCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

706

Amino Acids

78.54

Weight (kDa)

5.6

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 123 - 201 2.2e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 123 - 268 4.2e-10 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 123 - 207 2.1e-07 WDHD1 first WD40 domain
Beta-prop_WDR3_1st PF25173 124 - 200 1.9e-11 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 125 - 200 1.3e-12 WDR5 beta-propeller domain
WDR55 PF24796 125 - 239 8.8e-09 WDR55
EIF3I PF24805 128 - 200 1.2e-08 EIF3I
Beta-prop_TEP1_2nd PF25047 128 - 248 7.9e-07 TEP-1 second beta-propeller
WD40_Gbeta PF25391 133 - 199 4.6e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR41 PF25178 135 - 202 2.4e-08 WDR41
WD40_CDC20-Fz PF24807 135 - 202 2e-06 CDC20/Fizzy WD40 domain
DEAD PF00270 357 - 519 2.2e-42 DEAD/DEAH box helicase
ResIII PF04851 375 - 515 3e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 558 - 666 2e-31 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 131, 1790
Acc36I ACCTGC 1 cut(s) 1035
AccB1I GGYRCC 2 cut(s) 889, 1219
AccB7I CCANNNNNTGG 1 cut(s) 1210
AccII CGCG 1 cut(s) 916
AciI CCGC 3 cut(s) 93, 239, 887
AclWI GGATC 2 cut(s) 147, 160
AcoI YGGCCR 2 cut(s) 298, 1707
AcsI RAATTY 3 cut(s) 290, 664, 1847
AcuI CTGAAG 1 cut(s) 915
AdeI CACNNNGTG 1 cut(s) 1686
AfaI GTAC 2 cut(s) 5, 1911
AfiI CCNNNNNNNGG 3 cut(s) 1033, 1210, 1444
AflIII ACRYGT 1 cut(s) 914
AgsI TTSAA 6 cut(s) 71, 590, 622, 1175, 1474, 2097
AhlI ACTAGT 1 cut(s) 1313
AjnI CCWGG 5 cut(s) 95, 1203, 1372, 1557, 1623
AloI GAACNNNNNNTCC 4 cut(s) 570, 602, 1302, 1334
AluBI AGCT 8 cut(s) 193, 221, 638, 1180, 1234, 1501, 1675, 2089
AluI AGCT 8 cut(s) 193, 221, 638, 1180, 1234, 1501, 1675, 2089
Alw26I GTCTC 5 cut(s) 190, 772, 1798, 1803, 1824
AlwI GGATC 2 cut(s) 147, 160
AlwNI CAGNNNCTG 1 cut(s) 1796
Ama87I CYCGRG 2 cut(s) 1227, 1464
AoxI GGCC 5 cut(s) 272, 298, 1263, 1554, 1707
ApaI GGGCCC 1 cut(s) 1267
ApeKI GCWGC 2 cut(s) 410, 2089
ApoI RAATTY 3 cut(s) 290, 664, 1847
ArsI GACNNNNNNTTYG 2 cut(s) 1392, 1424
Asp700I GAANNNNTTC 1 cut(s) 48
AspLEI GCGC 1 cut(s) 265
AsuC2I CCSGG 1 cut(s) 1781
AsuHPI GGTGA 3 cut(s) 733, 1612, 1870
AsuII TTCGAA 1 cut(s) 854
AvaI CYCGRG 2 cut(s) 1227, 1464
AvaII GGWCC 6 cut(s) 332, 396, 627, 1477, 1783, 1816
AxyI CCTNAGG 1 cut(s) 1551
BaeGI GKGCMC 3 cut(s) 480, 748, 1267
BalI TGGCCA 2 cut(s) 300, 1709
BamHI GGATCC 1 cut(s) 152
BanI GGYRCC 2 cut(s) 889, 1219
BanII GRGCYC 2 cut(s) 103, 1267
BauI CACGAG 2 cut(s) 1866, 1896
BbsI GAAGAC 3 cut(s) 1151, 1332, 1405
BbvCI CCTCAGC 1 cut(s) 1031
BbvI GCAGC 2 cut(s) 397, 2076
BccI CCATC 6 cut(s) 137, 143, 179, 539, 950, 1189
BceAI ACGGC 3 cut(s) 22, 567, 687
BciT130I CCWGG 5 cut(s) 97, 1205, 1374, 1559, 1625
BclI TGATCA 2 cut(s) 1417, 1875
BcnI CCSGG 1 cut(s) 1781
BcoDI GTCTC 5 cut(s) 190, 772, 1798, 1803, 1824
BcuI ACTAGT 1 cut(s) 1313
BfaI CTAG 2 cut(s) 200, 1314
BfmI CTRYAG 1 cut(s) 37
BfuAI ACCTGC 1 cut(s) 1035
BglI GCCNNNNNGGC 1 cut(s) 306
BglII AGATCT 1 cut(s) 1482
BisI GCNGC 3 cut(s) 411, 888, 2090
BlsI GCNGC 3 cut(s) 412, 889, 2091
Bme1390I CCNGG 6 cut(s) 97, 1205, 1374, 1559, 1625, 1781
Bme18I GGWCC 6 cut(s) 332, 396, 627, 1477, 1783, 1816
BmeT110I CYCGRG 2 cut(s) 1227, 1464
BmrFI CCNGG 6 cut(s) 97, 1205, 1374, 1559, 1625, 1781
BmrI ACTGGG 2 cut(s) 31, 1149
BmsI GCATC 1 cut(s) 788
BmuI ACTGGG 2 cut(s) 31, 1149
BpiI GAAGAC 3 cut(s) 1151, 1332, 1405
BplI GAGNNNNNCTC 2 cut(s) 1049, 1081
BpmI CTGGAG 3 cut(s) 127, 1356, 1580
Bpu10I CCTNAGC 1 cut(s) 1031
Bpu14I TTCGAA 1 cut(s) 854
BpuEI CTTGAG 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 1781
BsaAI YACGTR 1 cut(s) 557
BsaXI ACNNNNNCTCC 2 cut(s) 801, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 1033, 1210, 1444
Bse1I ACTGG 3 cut(s) 26, 538, 1144
Bse21I CCTNAGG 1 cut(s) 1551
Bse3DI GCAATG 2 cut(s) 217, 746
BseBI CCWGG 5 cut(s) 97, 1205, 1374, 1559, 1625
BseGI GGATG 5 cut(s) 171, 445, 550, 872, 2038
BseLI CCNNNNNNNGG 3 cut(s) 1033, 1210, 1444
BseMI GCAATG 2 cut(s) 217, 746
BseMII CTCAG 5 cut(s) 340, 764, 1022, 1542, 1947
BseNI ACTGG 3 cut(s) 26, 538, 1144
BseRI GAGGAG 5 cut(s) 326, 825, 1679, 2099, 2102
BseSI GKGCMC 3 cut(s) 480, 748, 1267
BseXI GCAGC 2 cut(s) 397, 2076
BseYI CCCAGC 2 cut(s) 435, 1950
Bsh1236I CGCG 1 cut(s) 916
BshFI GGCC 5 cut(s) 274, 300, 1265, 1556, 1709
BshNI GGYRCC 2 cut(s) 889, 1219
BsiHKCI CYCGRG 2 cut(s) 1227, 1464
BsiSI CCGG 1 cut(s) 1780
BslFI GGGAC 4 cut(s) 38, 562, 1076, 1796
BslI CCNNNNNNNGG 3 cut(s) 1033, 1210, 1444
BsmAI GTCTC 5 cut(s) 190, 772, 1798, 1803, 1824
BsmBI CGTCTC 1 cut(s) 190
BsmFI GGGAC 4 cut(s) 38, 562, 1076, 1796
BsmI GAATGC 2 cut(s) 493, 2049
BsnI GGCC 5 cut(s) 274, 300, 1265, 1556, 1709
BsoBI CYCGRG 2 cut(s) 1227, 1464
Bsp119I TTCGAA 1 cut(s) 854
Bsp120I GGGCCC 1 cut(s) 1263
Bsp1286I GDGCHC 4 cut(s) 103, 480, 748, 1267
Bsp1407I TGTACA 1 cut(s) 1909
Bsp19I CCATGG 2 cut(s) 1008, 1804
BspACI CCGC 3 cut(s) 93, 239, 887
BspANI GGCC 5 cut(s) 274, 300, 1265, 1556, 1709
BspCNI CTCAG 5 cut(s) 339, 763, 1023, 1543, 1948
BspFNI CGCG 1 cut(s) 916
BspHI TCATGA 3 cut(s) 1257, 1578, 1839
BspMI ACCTGC 1 cut(s) 1035
BspPI GGATC 2 cut(s) 147, 160
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 2 cut(s) 889, 1219
BsrDI GCAATG 2 cut(s) 217, 746
BsrGI TGTACA 1 cut(s) 1909
BsrI ACTGG 3 cut(s) 26, 538, 1144
BssSI CACGAG 2 cut(s) 1866, 1896
BssT1I CCWWGG 5 cut(s) 335, 1008, 1267, 1704, 1804
Bst2BI CACGAG 2 cut(s) 1866, 1896
Bst2UI CCWGG 5 cut(s) 97, 1205, 1374, 1559, 1625
Bst4CI ACNGT 7 cut(s) 82, 880, 906, 998, 1052, 1404, 1792
Bst6I CTCTTC 1 cut(s) 856
BstAUI TGTACA 1 cut(s) 1909
BstBAI YACGTR 1 cut(s) 557
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 7 cut(s) 276, 415, 948, 1016, 1125, 1236, 1296
BstDEI CTNAG 7 cut(s) 326, 698, 750, 1031, 1394, 1551, 1956
BstDSI CCRYGG 3 cut(s) 301, 1008, 1804
BstENI CCTNNNNNAGG 1 cut(s) 1442
BstF5I GGATG 5 cut(s) 171, 445, 550, 872, 2038
BstFNI CGCG 1 cut(s) 916
BstHHI GCGC 1 cut(s) 265
BstMAI GTCTC 5 cut(s) 190, 772, 1798, 1803, 1824
BstMWI GCNNNNNNNGC 4 cut(s) 271, 306, 683, 1951
BstNI CCWGG 5 cut(s) 97, 1205, 1374, 1559, 1625
BstNSI RCATGY 2 cut(s) 1298, 1302
BstSCI CCNGG 6 cut(s) 95, 1203, 1372, 1557, 1623, 1779
BstSFI CTRYAG 1 cut(s) 37
BstSLI GKGCMC 3 cut(s) 480, 748, 1267
BstUI CGCG 1 cut(s) 916
BstV1I GCAGC 2 cut(s) 397, 2076
BstV2I GAAGAC 3 cut(s) 1151, 1332, 1405
BstX2I RGATCY 2 cut(s) 152, 1482
BstXI CCANNNNNNTGG 2 cut(s) 883, 1976
BstYI RGATCY 2 cut(s) 152, 1482
Bsu36I CCTNAGG 1 cut(s) 1551
BsuRI GGCC 5 cut(s) 274, 300, 1265, 1556, 1709
BtgI CCRYGG 3 cut(s) 301, 1008, 1804
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 5 cut(s) 171, 445, 550, 872, 2038
BveI ACCTGC 1 cut(s) 1035
Cac8I GCNNGC 7 cut(s) 276, 415, 948, 1016, 1125, 1236, 1296
CaiI CAGNNNCTG 1 cut(s) 1796
CciI TCATGA 3 cut(s) 1257, 1578, 1839
CfoI GCGC 1 cut(s) 265
CseI GACGC 3 cut(s) 20, 905, 1752
Csp6I GTAC 2 cut(s) 4, 1910
CviQI GTAC 2 cut(s) 4, 1910
DdeI CTNAG 7 cut(s) 326, 698, 750, 1031, 1394, 1551, 1956
DraIII CACNNNGTG 1 cut(s) 1686
DrdI GACNNNNNNGTC 2 cut(s) 131, 1790
DseDI GACNNNNNNGTC 2 cut(s) 131, 1790
EaeI YGGCCR 2 cut(s) 298, 1707
Eam1104I CTCTTC 1 cut(s) 856
EarI CTCTTC 1 cut(s) 856
EciI GGCGGA 1 cut(s) 254
Eco130I CCWWGG 5 cut(s) 335, 1008, 1267, 1704, 1804
Eco24I GRGCYC 2 cut(s) 103, 1267
Eco32I GATATC 1 cut(s) 2056
Eco47I GGWCC 6 cut(s) 332, 396, 627, 1477, 1783, 1816
Eco57I CTGAAG 1 cut(s) 915
Eco81I CCTNAGG 1 cut(s) 1551
Eco88I CYCGRG 2 cut(s) 1227, 1464
EcoNI CCTNNNNNAGG 1 cut(s) 1442
EcoO109I RGGNCCY 5 cut(s) 272, 332, 1263, 1264, 1555
EcoRI GAATTC 1 cut(s) 1847
EcoRII CCWGG 5 cut(s) 95, 1203, 1372, 1557, 1623
EcoRV GATATC 1 cut(s) 2056
EcoT14I CCWWGG 5 cut(s) 335, 1008, 1267, 1704, 1804
EcoT22I ATGCAT 1 cut(s) 1300
EcoT38I GRGCYC 2 cut(s) 103, 1267
ErhI CCWWGG 5 cut(s) 335, 1008, 1267, 1704, 1804
Esp3I CGTCTC 1 cut(s) 190
FalI AAGNNNNNCTT 2 cut(s) 1908, 1940
FaqI GGGAC 4 cut(s) 38, 562, 1076, 1796
FauNDI CATATG 1 cut(s) 280
FbaI TGATCA 2 cut(s) 1417, 1875
Fnu4HI GCNGC 3 cut(s) 411, 888, 2090
FokI GGATG 5 cut(s) 158, 452, 557, 859, 2045
FriOI GRGCYC 2 cut(s) 103, 1267
Fsp4HI GCNGC 3 cut(s) 411, 888, 2090
FspBI CTAG 2 cut(s) 200, 1314
GlaI GCGC 1 cut(s) 264
GluI GCNGC 3 cut(s) 411, 888, 2090
GsaI CCCAGC 2 cut(s) 439, 1954
GsuI CTGGAG 3 cut(s) 127, 1356, 1580
HaeIII GGCC 5 cut(s) 274, 300, 1265, 1556, 1709
HapII CCGG 1 cut(s) 1780
HgaI GACGC 3 cut(s) 20, 905, 1752
HhaI GCGC 1 cut(s) 265
Hin6I GCGC 1 cut(s) 263
HinP1I GCGC 1 cut(s) 263
HincII GTYRAC 1 cut(s) 1761
HindII GTYRAC 1 cut(s) 1761
HindIII AAGCTT 1 cut(s) 1673
HinfI GANTC 8 cut(s) 67, 314, 419, 724, 1004, 1117, 1516, 1597
HpaII CCGG 1 cut(s) 1780
HphI GGTGA 3 cut(s) 733, 1612, 1870
Hpy166II GTNNAC 4 cut(s) 902, 991, 1657, 1761
Hpy188I TCNGA 5 cut(s) 89, 484, 525, 541, 2117
Hpy8I GTNNAC 4 cut(s) 902, 991, 1657, 1761
Hpy99I CGWCG 4 cut(s) 14, 137, 140, 1746
HpyAV CCTTC 7 cut(s) 848, 890, 1165, 1246, 1622, 1996, 2103
HpyCH4III ACNGT 7 cut(s) 82, 880, 906, 998, 1052, 1404, 1792
HpyCH4IV ACGT 3 cut(s) 285, 556, 1333
HpyF10VI GCNNNNNNNGC 4 cut(s) 271, 306, 683, 1951
HpyF3I CTNAG 7 cut(s) 326, 698, 750, 1031, 1394, 1551, 1956
HpySE526I ACGT 3 cut(s) 285, 556, 1333
HspAI GCGC 1 cut(s) 263
Ksp22I TGATCA 2 cut(s) 1417, 1875
LmnI GCTCC 6 cut(s) 58, 106, 198, 226, 683, 2086
Lsp1109I GCAGC 2 cut(s) 397, 2076
LweI GCATC 1 cut(s) 788
MaeI CTAG 2 cut(s) 200, 1314
MaeII ACGT 3 cut(s) 285, 556, 1333
MaeIII GTNAC 3 cut(s) 787, 1404, 2023
MboII GAAGA 8 cut(s) 41, 634, 843, 1156, 1163, 1337, 1410, 2001
MflI RGATCY 2 cut(s) 152, 1482
MhlI GDGCHC 4 cut(s) 103, 480, 748, 1267
MlsI TGGCCA 2 cut(s) 300, 1709
MluCI AATT 9 cut(s) 247, 290, 354, 617, 664, 1086, 1632, 1847, 1974
MluI ACGCGT 1 cut(s) 914
MluNI TGGCCA 2 cut(s) 300, 1709
MlyI GAGTC 1 cut(s) 323
MmeI TCCRAC 2 cut(s) 661, 1345
Mox20I TGGCCA 2 cut(s) 300, 1709
Mph1103I ATGCAT 1 cut(s) 1300
MroXI GAANNNNTTC 1 cut(s) 48
MscI TGGCCA 2 cut(s) 300, 1709
MslI CAYNNNNRTG 5 cut(s) 559, 881, 932, 1299, 1838
Msp20I TGGCCA 2 cut(s) 300, 1709
MspI CCGG 1 cut(s) 1780
MspR9I CCNGG 6 cut(s) 97, 1205, 1374, 1559, 1625, 1781
Mva1269I GAATGC 2 cut(s) 493, 2049
MvaI CCWGG 5 cut(s) 97, 1205, 1374, 1559, 1625
MvnI CGCG 1 cut(s) 916
MwoI GCNNNNNNNGC 4 cut(s) 271, 306, 683, 1951
NciI CCSGG 1 cut(s) 1781
NcoI CCATGG 2 cut(s) 1008, 1804
NdeI CATATG 1 cut(s) 280
NmuCI GTSAC 2 cut(s) 787, 1404
NsiI ATGCAT 1 cut(s) 1300
NspI RCATGY 2 cut(s) 1298, 1302
NspV TTCGAA 1 cut(s) 854
PaeI GCATGC 1 cut(s) 1298
PaeR7I CTCGAG 1 cut(s) 1227
PagI TCATGA 3 cut(s) 1257, 1578, 1839
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 2 cut(s) 493, 2049
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 7 cut(s) 67, 419, 724, 1004, 1117, 1516, 1597
PflMI CCANNNNNTGG 1 cut(s) 1210
PkrI GCNGC 3 cut(s) 412, 889, 2091
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 557
PpuMI RGGWCCY 1 cut(s) 332
Psp5II RGGWCCY 1 cut(s) 332
Psp6I CCWGG 5 cut(s) 95, 1203, 1372, 1557, 1623
PspFI CCCAGC 2 cut(s) 435, 1950
PspGI CCWGG 5 cut(s) 95, 1203, 1372, 1557, 1623
PspOMI GGGCCC 1 cut(s) 1263
PspPPI RGGWCCY 1 cut(s) 332
PstNI CAGNNNCTG 1 cut(s) 1796
PsuI RGATCY 2 cut(s) 152, 1482
RsaI GTAC 2 cut(s) 5, 1911
RsaNI GTAC 2 cut(s) 4, 1910
RseI CAYNNNNRTG 5 cut(s) 559, 881, 932, 1299, 1838
SatI GCNGC 3 cut(s) 411, 888, 2090
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 6 cut(s) 97, 1205, 1374, 1559, 1625, 1781
SduI GDGCHC 4 cut(s) 103, 480, 748, 1267
SfaNI GCATC 1 cut(s) 788
SfcI CTRYAG 1 cut(s) 37
Sfr274I CTCGAG 1 cut(s) 1227
SfuI TTCGAA 1 cut(s) 854
SinI GGWCC 6 cut(s) 332, 396, 627, 1477, 1783, 1816
SlaI CTCGAG 1 cut(s) 1227
SmiMI CAYNNNNRTG 5 cut(s) 559, 881, 932, 1299, 1838
SmlI CTYRAG 2 cut(s) 1181, 1227
SmoI CTYRAG 2 cut(s) 1181, 1227
SpeI ACTAGT 1 cut(s) 1313
SphI GCATGC 1 cut(s) 1298
Sse9I AATT 9 cut(s) 247, 290, 354, 617, 664, 1086, 1632, 1847, 1974
SsiI CCGC 3 cut(s) 93, 239, 887
SspMI CTAG 2 cut(s) 200, 1314
StyD4I CCNGG 6 cut(s) 95, 1203, 1372, 1557, 1623, 1779
StyI CCWWGG 5 cut(s) 335, 1008, 1267, 1704, 1804
TaaI ACNGT 7 cut(s) 82, 880, 906, 998, 1052, 1404, 1792
TaiI ACGT 3 cut(s) 288, 559, 1336
TaqI TCGA 5 cut(s) 825, 854, 924, 1228, 1741
TaqII GACCGA 1 cut(s) 1778
TasI AATT 9 cut(s) 247, 290, 354, 617, 664, 1086, 1632, 1847, 1974
TatI WGTACW 1 cut(s) 1909
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 7 cut(s) 67, 419, 724, 1004, 1117, 1516, 1597
TseFI GTSAC 2 cut(s) 787, 1404
TseI GCWGC 2 cut(s) 410, 2089
Tsp45I GTSAC 2 cut(s) 787, 1404
TspDTI ATGAA 8 cut(s) 950, 999, 1343, 1467, 1567, 1595, 1629, 1713
TspGWI ACGGA 2 cut(s) 145, 1841
Van91I CCANNNNNTGG 1 cut(s) 1210
VpaK11BI GGWCC 6 cut(s) 332, 396, 627, 1477, 1783, 1816
XagI CCTNNNNNAGG 1 cut(s) 1442
XapI RAATTY 3 cut(s) 290, 664, 1847
XceI RCATGY 2 cut(s) 1298, 1302
XcmI CCANNNNNNNNNTGG 1 cut(s) 1888
XhoI CTCGAG 1 cut(s) 1227
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 2 cut(s) 200, 1314
Zsp2I ATGCAT 1 cut(s) 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.