pycom17g17080

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000799
Physical Location & Seq
Forward (+)
14960232 .. 14960736
505 bp
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UTR
Exon/CDS
Intron
pycom17g17080.1

Sequence Viewer

Length: 408 bp
ATGCTTTCTCGTGGTTTTAAGGATCAGATCTACGACATTTTCCAGCTTCTGCCATCAAAGGTTCAGGTTGGGGTGTTTTCTGCTACCATGCCACCTGAAGCCCTGGAAATCACAAGGAAGTTTATGAACAAACCCGTGAGGATCTTGGTAAAGCGTGATGAGCTCACCCTTGAGGGTATCAAGCAGTTCTATGTCAATGTCGATAAAGAAGAATGGAAGCTCGAGACCCTTTGTGATCTGTATGAGACCCTGGCCATCACCCAGAGTGTCATTTTCGTTAACACCAGGCGCAAGGTGGACTGGCTTACTGACAAGATGAGAAGCCGTGACCACACTGTCTCCGCCACCCACGGTGACATGGACCAGAACACTCGTGACATCATCATGCGTGAATTCCGATCGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

136

Amino Acids

15.93

Weight (kDa)

7.85

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 72 - 133 2e-08 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AciI CCGC 1 cut(s) 342
AclWI GGATC 2 cut(s) 30, 149
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 117
AjnI CCWGG 3 cut(s) 102, 249, 284
AluBI AGCT 3 cut(s) 46, 163, 220
AluI AGCT 3 cut(s) 46, 163, 220
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 3 cut(s) 218, 239, 343
AlwI GGATC 2 cut(s) 30, 149
AlwNI CAGNNNCTG 1 cut(s) 49
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 1 cut(s) 252
ApoI RAATTY 1 cut(s) 392
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 1 cut(s) 361
AsuHPI GGTGA 3 cut(s) 157, 250, 365
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 1 cut(s) 361
BalI TGGCCA 1 cut(s) 254
BanII GRGCYC 1 cut(s) 165
BauI CACGAG 2 cut(s) 9, 372
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 2 cut(s) 61, 263
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 3 cut(s) 104, 251, 286
BcoDI GTCTC 3 cut(s) 218, 239, 343
BglII AGATCT 1 cut(s) 27
Bme1390I CCNGG 3 cut(s) 104, 251, 286
Bme18I GGWCC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 361
BmrFI CCNGG 3 cut(s) 104, 251, 286
BpuEI CTTGAG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 401
BsaI GGTCTC 2 cut(s) 218, 239
BsaJI CCNNGG 3 cut(s) 102, 249, 349
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bse1I ACTGG 1 cut(s) 305
BseBI CCWGG 3 cut(s) 104, 251, 286
BseCI ATCGAT 1 cut(s) 401
BseDI CCNNGG 3 cut(s) 102, 249, 349
BseNI ACTGG 1 cut(s) 305
Bsh1285I CGRYCG 1 cut(s) 401
BshFI GGCC 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 401
BsiEI CGRYCG 1 cut(s) 401
BsiHKAI GWGCWC 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 221
BsmAI GTCTC 3 cut(s) 218, 239, 343
BsnI GGCC 1 cut(s) 254
Bso31I GGTCTC 2 cut(s) 218, 239
BsoBI CYCGRG 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 165
Bsp143I GATC 6 cut(s) 22, 27, 141, 235, 398, 402
BspACI CCGC 1 cut(s) 342
BspANI GGCC 1 cut(s) 254
BspDI ATCGAT 1 cut(s) 401
BspPI GGATC 2 cut(s) 30, 149
BspTNI GGTCTC 2 cut(s) 218, 239
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 3 cut(s) 102, 249, 349
BssMI GATC 6 cut(s) 22, 27, 141, 235, 398, 402
BssSI CACGAG 2 cut(s) 9, 372
Bst2BI CACGAG 2 cut(s) 9, 372
Bst2UI CCWGG 3 cut(s) 104, 251, 286
Bst4CI ACNGT 2 cut(s) 337, 353
BstDSI CCRYGG 1 cut(s) 349
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 6 cut(s) 25, 30, 144, 238, 401, 405
BstMAI GTCTC 3 cut(s) 218, 239, 343
BstMBI GATC 6 cut(s) 22, 27, 141, 235, 398, 402
BstMCI CGRYCG 1 cut(s) 401
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstNI CCWGG 3 cut(s) 104, 251, 286
BstSCI CCNGG 3 cut(s) 102, 249, 284
BstX2I RGATCY 2 cut(s) 27, 141
BstYI RGATCY 2 cut(s) 27, 141
Bsu15I ATCGAT 1 cut(s) 401
BsuRI GGCC 1 cut(s) 254
BsuTUI ATCGAT 1 cut(s) 401
BtgI CCRYGG 1 cut(s) 349
BtsIMutI CAGTG 1 cut(s) 333
CaiI CAGNNNCTG 1 cut(s) 49
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 1 cut(s) 361
ClaI ATCGAT 1 cut(s) 401
CviAII CATG 3 cut(s) 88, 358, 385
CviJI RGCY 7 cut(s) 46, 101, 163, 220, 254, 304, 324
CviKI_1 RGCY 7 cut(s) 46, 101, 163, 220, 254, 304, 324
DpnI GATC 6 cut(s) 24, 29, 143, 237, 400, 404
DpnII GATC 6 cut(s) 22, 27, 141, 235, 398, 402
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 252
EciI GGCGGA 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 163
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 2 cut(s) 218, 239
Eco47I GGWCC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 221
EcoICRI GAGCTC 1 cut(s) 163
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 3 cut(s) 102, 249, 284
EcoT38I GRGCYC 1 cut(s) 165
FaeI CATG 3 cut(s) 91, 361, 388
FaiI YATR 6 cut(s) 89, 125, 192, 243, 359, 386
FatI CATG 3 cut(s) 87, 357, 384
FriOI GRGCYC 1 cut(s) 165
GlaI GCGC 1 cut(s) 290
HaeIII GGCC 1 cut(s) 254
HhaI GCGC 1 cut(s) 291
Hin1II CATG 3 cut(s) 91, 361, 388
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HpaI GTTAAC 1 cut(s) 280
HphI GGTGA 3 cut(s) 157, 250, 365
Hpy166II GTNNAC 2 cut(s) 280, 298
Hpy188I TCNGA 3 cut(s) 27, 398, 407
Hpy188III TCNNGA 2 cut(s) 223, 374
Hpy8I GTNNAC 2 cut(s) 280, 298
HpyCH4III ACNGT 2 cut(s) 337, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
Hsp92II CATG 3 cut(s) 91, 361, 388
HspAI GCGC 1 cut(s) 289
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 6 cut(s) 22, 27, 141, 235, 398, 402
MaeIII GTNAC 3 cut(s) 326, 353, 374
MalI GATC 6 cut(s) 24, 29, 143, 237, 400, 404
MboI GATC 6 cut(s) 22, 27, 141, 235, 398, 402
MboII GAAGA 1 cut(s) 221
MflI RGATCY 2 cut(s) 27, 141
MhlI GDGCHC 1 cut(s) 165
MlsI TGGCCA 1 cut(s) 254
MluCI AATT 1 cut(s) 392
MluNI TGGCCA 1 cut(s) 254
MnlI CCTC 2 cut(s) 132, 166
Mox20I TGGCCA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 254
MseI TTAA 2 cut(s) 18, 279
MslI CAYNNNNRTG 1 cut(s) 383
Msp20I TGGCCA 1 cut(s) 254
MspR9I CCNGG 3 cut(s) 104, 251, 286
MvaI CCWGG 3 cut(s) 104, 251, 286
MwoI GCNNNNNNNGC 1 cut(s) 160
NdeII GATC 6 cut(s) 22, 27, 141, 235, 398, 402
NlaIII CATG 3 cut(s) 91, 361, 388
NmuCI GTSAC 3 cut(s) 326, 353, 374
PaeR7I CTCGAG 1 cut(s) 221
Ple19I CGATCG 1 cut(s) 401
Psp124BI GAGCTC 1 cut(s) 165
Psp6I CCWGG 3 cut(s) 102, 249, 284
PspGI CCWGG 3 cut(s) 102, 249, 284
PspPI GGNCC 1 cut(s) 361
PstNI CAGNNNCTG 1 cut(s) 49
PsuI RGATCY 2 cut(s) 27, 141
PvuI CGATCG 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 383
SacI GAGCTC 1 cut(s) 165
SaqAI TTAA 2 cut(s) 18, 279
Sau3AI GATC 6 cut(s) 22, 27, 141, 235, 398, 402
Sau96I GGNCC 1 cut(s) 361
ScrFI CCNGG 3 cut(s) 104, 251, 286
SduI GDGCHC 1 cut(s) 165
SetI ASST 7 cut(s) 48, 63, 69, 97, 165, 222, 297
Sfr274I CTCGAG 1 cut(s) 221
SinI GGWCC 1 cut(s) 361
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 2 cut(s) 170, 221
SmoI CTYRAG 2 cut(s) 170, 221
Sse9I AATT 1 cut(s) 392
SsiI CCGC 1 cut(s) 342
SstI GAGCTC 1 cut(s) 165
StyD4I CCNGG 3 cut(s) 102, 249, 284
TaaI ACNGT 2 cut(s) 337, 353
TaqI TCGA 3 cut(s) 201, 222, 401
TasI AATT 1 cut(s) 392
Tru1I TTAA 2 cut(s) 18, 279
Tru9I TTAA 2 cut(s) 18, 279
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 3 cut(s) 326, 353, 374
Tsp45I GTSAC 3 cut(s) 326, 353, 374
TspDTI ATGAA 1 cut(s) 140
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 1 cut(s) 361
XapI RAATTY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 292
XhoI CTCGAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.