Rorug02G0386400

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000799
Physical Location & Seq
Reverse (-)
49308595 .. 49310489
1895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0386400.1

Sequence Viewer

Length: 1284 bp
ATGGCTACAGATGTAATACTGCAAAACACTGTGGAAGAGGAGGAAAACCAGCATGGGGATCAAGTCTCTGAACAGCTCAAAGAGAATCAACTAGTTTCAAGCCAAAAGTTTCAAAGATTTGACTCCTTGGAGGCTTGGACATCGAGTCTTGCAGTATCGCTGATCACAGGCACTCCAAGTTTGTCTCATGAAGTGACGTGGTCCGTAATACTGACCCTGGCATTTCAGAGCATTGGAGTTGTATATGGAGATCTTGGTACTTCACCACTTTATGTGTTCTCGAGCACCTTCCCCAATGGAATCAAAGATAAAGATGACATCTTGGGTGTTCTTTCTTTGATTCTTTATACAGTCACATTAATCCCCTTGATTAAGTATGTTCTCATCGTCCTACAAATGATAACGGCGATGGGATGGCGAATTCTTTCCAACCCTCAATCTTTGATTGCCAGGCTATATAAGGCTAAATACTTCCCAAATTGTTCCTTTTGGGAAGCAGAAGTTGGGGACTCACCTTCCTTTTCATGGAGAAGTATTCTGCACGGTAGGTCGGTTCTCAAGGCAGGAGTGCAGTGGAAGATTGGAGATGGGAAATCTGTTAACATATGGCATGACAATTGGTTGCCAATTCAGGGTTCTCCCATCATTCACCAACTGCCAAACTCAACCCCAAGTACTGTGGCTGAACTGATTGATTCAACCACTAGGCAATGGAAAACTGACATTGTGTATAGCCTATTCCCAACAGAGGTTGCGGATCGCATTTTATGCATTCCAATTAGTAGACAGCCTACAGTGGATAGGCTTATTTGGAATCCGGAAAAACGCGGTTTCTACTCGGTTAAATCTGCTTATTGGATTGCCCGCAATGAGGTAATGCACCATGTCCTAGTCTCAACATCTACTGGAGACCCCTATAAGGATTTGTGGCGCAGTCTTTGGAGGGCTAAAGTTCCTGGTAAGGTTAGCATTTGTGTTTGGCGTGCATGCCTGAACCTTTTACCTACACGTGATCGTCTATTTACTAAAGGTTATGAGGGGCCTATGAGATGTGTGATATGCAACAATCAAGTTGAAGATGTTGTTCATGTGTTCTGCAAGTGTCCGGTAGCTTCTGCGATTCTACATGCTTCACCATTTAACTTACAGCACACCATCCTCCCTGCCCTTAACTTTAAGGAATGGATGCTAGAAAGGGCAACCACTATGCAGCCTCATCTCTTTGCTCAACTACTCATGACACTACACCAATTTTGTTATCAGACAACACTTTTTGCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

427

Amino Acids

48.49

Weight (kDa)

7.62

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 73 - 133 1.2e-18 K+ potassium transporter integral membrane domain
zf-RVT PF13966 279 - 373 7.5e-17 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 784
AccII CGCG 1 cut(s) 828
AccIII TCCGGA 1 cut(s) 817
AciI CCGC 3 cut(s) 755, 828, 865
AclWI GGATC 2 cut(s) 66, 765
AcsI RAATTY 1 cut(s) 420
AcvI CACGTG 1 cut(s) 1010
AfaI GTAC 2 cut(s) 259, 676
AfiI CCNNNNNNNGG 6 cut(s) 55, 525, 632, 748, 871, 919
AflIII ACRYGT 1 cut(s) 1007
AgsI TTSAA 4 cut(s) 99, 113, 699, 1076
AhdI GACNNNNNGTC 1 cut(s) 144
AhlI ACTAGT 1 cut(s) 91
AjiI CACGTC 1 cut(s) 198
AjnI CCWGG 3 cut(s) 216, 449, 955
AjuI GAANNNNNNNTTGG 4 cut(s) 486, 518, 619, 651
AluBI AGCT 2 cut(s) 76, 1112
AluI AGCT 2 cut(s) 76, 1112
Alw21I GWGCWC 1 cut(s) 287
Alw26I GTCTC 4 cut(s) 70, 189, 898, 903
AlwI GGATC 2 cut(s) 66, 765
Ama87I CYCGRG 1 cut(s) 280
Aor13HI TCCGGA 1 cut(s) 817
AoxI GGCC 1 cut(s) 1040
ApeKI GCWGC 1 cut(s) 1210
ApoI RAATTY 1 cut(s) 420
AseI ATTAAT 1 cut(s) 359
Asp700I GAANNNNTTC 1 cut(s) 424
AspLEI GCGC 1 cut(s) 933
AspS9I GGNCC 2 cut(s) 201, 1040
AsuHPI GGTGA 4 cut(s) 255, 504, 641, 1125
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 1 cut(s) 201
BbrPI CACGTG 1 cut(s) 1010
Bbv12I GWGCWC 1 cut(s) 287
BbvI GCAGC 1 cut(s) 1222
BccI CCATC 5 cut(s) 403, 408, 581, 650, 1163
BceAI ACGGC 1 cut(s) 420
BciT130I CCWGG 3 cut(s) 218, 451, 957
BclI TGATCA 1 cut(s) 162
BcoDI GTCTC 4 cut(s) 70, 189, 898, 903
BcuI ACTAGT 1 cut(s) 91
BfaI CTAG 4 cut(s) 92, 705, 890, 1190
BfmI CTRYAG 2 cut(s) 6, 792
BglII AGATCT 1 cut(s) 250
BisI GCNGC 1 cut(s) 1211
BlsI GCNGC 1 cut(s) 1212
BmcAI AGTACT 1 cut(s) 676
Bme1390I CCNGG 3 cut(s) 218, 451, 957
Bme18I GGWCC 1 cut(s) 201
BmeRI GACNNNNNGTC 1 cut(s) 144
BmeT110I CYCGRG 1 cut(s) 280
BmgBI CACGTC 1 cut(s) 198
BmgT120I GGNCC 2 cut(s) 201, 1040
BmiI GGNNCC 1 cut(s) 1041
BmrFI CCNGG 3 cut(s) 218, 451, 957
BmsI GCATC 1 cut(s) 1176
BpmI CTGGAG 1 cut(s) 927
BpuEI CTTGAG 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 1010
BsaI GGTCTC 1 cut(s) 903
BsaJI CCNNGG 2 cut(s) 126, 216
BsaWI WCCGGW 2 cut(s) 817, 1105
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bsc4I CCNNNNNNNGG 6 cut(s) 55, 525, 632, 748, 871, 919
Bse1I ACTGG 1 cut(s) 910
Bse3DI GCAATG 2 cut(s) 716, 874
BseAI TCCGGA 1 cut(s) 817
BseBI CCWGG 3 cut(s) 218, 451, 957
BseDI CCNNGG 2 cut(s) 126, 216
BseGI GGATG 3 cut(s) 419, 1155, 1191
BseLI CCNNNNNNNGG 6 cut(s) 55, 525, 632, 748, 871, 919
BseMI GCAATG 2 cut(s) 716, 874
BseNI ACTGG 1 cut(s) 910
BseRI GAGGAG 1 cut(s) 53
BseXI GCAGC 1 cut(s) 1222
BsgI GTGCAG 2 cut(s) 524, 590
Bsh1236I CGCG 1 cut(s) 828
BshFI GGCC 1 cut(s) 1042
BsiHKAI GWGCWC 1 cut(s) 287
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 818, 1106
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 6 cut(s) 55, 525, 632, 748, 871, 919
BsmAI GTCTC 4 cut(s) 70, 189, 898, 903
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 1 cut(s) 771
BsnI GGCC 1 cut(s) 1042
Bso31I GGTCTC 1 cut(s) 903
BsoBI CYCGRG 1 cut(s) 280
Bsp1286I GDGCHC 1 cut(s) 287
Bsp13I TCCGGA 1 cut(s) 817
Bsp143I GATC 5 cut(s) 58, 162, 250, 757, 1012
BspACI CCGC 3 cut(s) 755, 828, 865
BspANI GGCC 1 cut(s) 1042
BspEI TCCGGA 1 cut(s) 817
BspFNI CGCG 1 cut(s) 828
BspHI TCATGA 2 cut(s) 187, 1236
BspLI GGNNCC 1 cut(s) 1041
BspPI GGATC 2 cut(s) 66, 765
BspTNI GGTCTC 1 cut(s) 903
BsrDI GCAATG 2 cut(s) 716, 874
BsrI ACTGG 1 cut(s) 910
BssECI CCNNGG 2 cut(s) 126, 216
BssMI GATC 5 cut(s) 58, 162, 250, 757, 1012
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 3 cut(s) 218, 451, 957
Bst4CI ACNGT 5 cut(s) 31, 352, 545, 679, 796
Bst6I CTCTTC 1 cut(s) 30
BstAPI GCANNNNNTGC 1 cut(s) 768
BstBAI YACGTR 1 cut(s) 1010
BstC8I GCNNGC 3 cut(s) 865, 984, 988
BstF5I GGATG 3 cut(s) 419, 1155, 1191
BstFNI CGCG 1 cut(s) 828
BstHHI GCGC 1 cut(s) 933
BstKTI GATC 5 cut(s) 61, 165, 253, 760, 1015
BstMAI GTCTC 4 cut(s) 70, 189, 898, 903
BstMBI GATC 5 cut(s) 58, 162, 250, 757, 1012
BstMWI GCNNNNNNNGC 1 cut(s) 768
BstNI CCWGG 3 cut(s) 218, 451, 957
BstNSI RCATGY 2 cut(s) 990, 1130
BstSCI CCNGG 3 cut(s) 216, 449, 955
BstSFI CTRYAG 2 cut(s) 6, 792
BstUI CGCG 1 cut(s) 828
BstV1I GCAGC 1 cut(s) 1222
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuRI GGCC 1 cut(s) 1042
BtgZI GCGATG 1 cut(s) 422
BtrI CACGTC 1 cut(s) 198
BtsCI GGATG 3 cut(s) 419, 1155, 1191
BtsI GCAGTG 1 cut(s) 578
BtsIMutI CAGTG 3 cut(s) 27, 578, 801
Cac8I GCNNGC 3 cut(s) 865, 984, 988
CciI TCATGA 2 cut(s) 187, 1236
CfoI GCGC 1 cut(s) 933
Cfr13I GGNCC 2 cut(s) 201, 1040
Csp6I GTAC 2 cut(s) 258, 675
CspCI CAANNNNNGTGG 2 cut(s) 660, 695
CviAII CATG 9 cut(s) 53, 188, 525, 611, 884, 987, 1088, 1127, 1237
CviQI GTAC 2 cut(s) 258, 675
DpnI GATC 5 cut(s) 60, 164, 252, 759, 1014
DpnII GATC 5 cut(s) 58, 162, 250, 757, 1012
DriI GACNNNNNGTC 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 30
Eam1105I GACNNNNNGTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 903
Eco47I GGWCC 1 cut(s) 201
Eco72I CACGTG 1 cut(s) 1010
Eco88I CYCGRG 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 1040
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 3 cut(s) 216, 449, 955
EcoT14I CCWWGG 1 cut(s) 126
EcoT22I ATGCAT 1 cut(s) 773
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 9 cut(s) 56, 191, 528, 614, 887, 990, 1091, 1130, 1240
FaqI GGGAC 1 cut(s) 521
FatI CATG 9 cut(s) 52, 187, 524, 610, 883, 986, 1087, 1126, 1236
FauI CCCGC 1 cut(s) 872
FauNDI CATATG 1 cut(s) 605
FbaI TGATCA 1 cut(s) 162
FblI GTMKAC 1 cut(s) 784
Fnu4HI GCNGC 1 cut(s) 1211
FokI GGATG 3 cut(s) 426, 1142, 1198
Fsp4HI GCNGC 1 cut(s) 1211
FspBI CTAG 4 cut(s) 92, 705, 890, 1190
GlaI GCGC 1 cut(s) 932
GluI GCNGC 1 cut(s) 1211
GsuI CTGGAG 1 cut(s) 927
HaeIII GGCC 1 cut(s) 1042
HapII CCGG 2 cut(s) 818, 1106
HhaI GCGC 1 cut(s) 933
Hin1II CATG 9 cut(s) 56, 191, 528, 614, 887, 990, 1091, 1130, 1240
Hin6I GCGC 1 cut(s) 931
HinP1I GCGC 1 cut(s) 931
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 9 cut(s) 85, 122, 145, 300, 340, 509, 695, 814, 1120
HpaI GTTAAC 1 cut(s) 601
HpaII CCGG 2 cut(s) 818, 1106
HphI GGTGA 4 cut(s) 255, 504, 641, 1125
Hpy166II GTNNAC 2 cut(s) 601, 785
Hpy188I TCNGA 3 cut(s) 70, 228, 1263
Hpy188III TCNNGA 4 cut(s) 188, 280, 818, 1237
Hpy8I GTNNAC 2 cut(s) 601, 785
HpyAV CCTTC 2 cut(s) 298, 525
HpyCH4III ACNGT 5 cut(s) 31, 352, 545, 679, 796
HpyCH4IV ACGT 2 cut(s) 197, 1009
HpyF10VI GCNNNNNNNGC 1 cut(s) 768
HpySE526I ACGT 2 cut(s) 197, 1009
Hsp92II CATG 9 cut(s) 56, 191, 528, 614, 887, 990, 1091, 1130, 1240
HspAI GCGC 1 cut(s) 931
Kpn2I TCCGGA 1 cut(s) 817
Ksp22I TGATCA 1 cut(s) 162
KspAI GTTAAC 1 cut(s) 601
Kzo9I GATC 5 cut(s) 58, 162, 250, 757, 1012
Lsp1109I GCAGC 1 cut(s) 1222
LweI GCATC 1 cut(s) 1176
MaeI CTAG 4 cut(s) 92, 705, 890, 1190
MaeII ACGT 2 cut(s) 197, 1009
MaeIII GTNAC 2 cut(s) 193, 352
MalI GATC 5 cut(s) 60, 164, 252, 759, 1014
MboI GATC 5 cut(s) 58, 162, 250, 757, 1012
MboII GAAGA 3 cut(s) 47, 589, 1088
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 6 cut(s) 420, 478, 616, 627, 777, 1250
MlyI GAGTC 3 cut(s) 116, 154, 503
MmeI TCCRAC 1 cut(s) 453
Mph1103I ATGCAT 1 cut(s) 773
MroI TCCGGA 1 cut(s) 817
MroXI GAANNNNTTC 1 cut(s) 424
MseI TTAA 7 cut(s) 359, 372, 600, 843, 1140, 1170, 1176
MspI CCGG 2 cut(s) 818, 1106
MspR9I CCNGG 3 cut(s) 218, 451, 957
MunI CAATTG 1 cut(s) 616
Mva1269I GAATGC 1 cut(s) 771
MvaI CCWGG 3 cut(s) 218, 451, 957
MvnI CGCG 1 cut(s) 828
MwoI GCNNNNNNNGC 1 cut(s) 768
NdeI CATATG 1 cut(s) 605
NdeII GATC 5 cut(s) 58, 162, 250, 757, 1012
NlaIII CATG 9 cut(s) 56, 191, 528, 614, 887, 990, 1091, 1130, 1240
NlaIV GGNNCC 1 cut(s) 1041
NmuCI GTSAC 2 cut(s) 193, 352
NsiI ATGCAT 1 cut(s) 773
NspI RCATGY 2 cut(s) 990, 1130
PaeI GCATGC 1 cut(s) 990
PaeR7I CTCGAG 1 cut(s) 280
PagI TCATGA 2 cut(s) 187, 1236
PctI GAATGC 1 cut(s) 771
PdmI GAANNNNTTC 1 cut(s) 424
PfeI GAWTC 6 cut(s) 85, 300, 340, 695, 814, 1120
PflFI GACNNNGTC 1 cut(s) 199
PkrI GCNGC 1 cut(s) 1212
PleI GAGTC 3 cut(s) 116, 153, 503
PmaCI CACGTG 1 cut(s) 1010
PmlI CACGTG 1 cut(s) 1010
PpsI GAGTC 3 cut(s) 116, 153, 503
Ppu21I YACGTR 1 cut(s) 1010
PshBI ATTAAT 1 cut(s) 359
Psp6I CCWGG 3 cut(s) 216, 449, 955
PspCI CACGTG 1 cut(s) 1010
PspGI CCWGG 3 cut(s) 216, 449, 955
PspN4I GGNNCC 1 cut(s) 1041
PspPI GGNCC 2 cut(s) 201, 1040
PsuI RGATCY 1 cut(s) 250
PsyI GACNNNGTC 1 cut(s) 199
RsaI GTAC 2 cut(s) 259, 676
RsaNI GTAC 2 cut(s) 258, 675
SaqAI TTAA 7 cut(s) 359, 372, 600, 843, 1140, 1170, 1176
SatI GCNGC 1 cut(s) 1211
Sau3AI GATC 5 cut(s) 58, 162, 250, 757, 1012
Sau96I GGNCC 2 cut(s) 201, 1040
ScaI AGTACT 1 cut(s) 676
SchI GAGTC 3 cut(s) 116, 154, 503
ScrFI CCNGG 3 cut(s) 218, 451, 957
SduI GDGCHC 1 cut(s) 287
SfaNI GCATC 1 cut(s) 1176
SfcI CTRYAG 2 cut(s) 6, 792
Sfr274I CTCGAG 1 cut(s) 280
SinI GGWCC 1 cut(s) 201
SlaI CTCGAG 1 cut(s) 280
SmlI CTYRAG 2 cut(s) 280, 557
SmoI CTYRAG 2 cut(s) 280, 557
SpeI ACTAGT 1 cut(s) 91
SphI GCATGC 1 cut(s) 990
Sse9I AATT 6 cut(s) 420, 478, 616, 627, 777, 1250
SsiI CCGC 3 cut(s) 755, 828, 865
SspMI CTAG 4 cut(s) 92, 705, 890, 1190
StyD4I CCNGG 3 cut(s) 216, 449, 955
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 5 cut(s) 31, 352, 545, 679, 796
TaiI ACGT 2 cut(s) 200, 1012
TaqI TCGA 2 cut(s) 143, 281
TasI AATT 6 cut(s) 420, 478, 616, 627, 777, 1250
TatI WGTACW 1 cut(s) 674
TfiI GAWTC 6 cut(s) 85, 300, 340, 695, 814, 1120
Tru1I TTAA 7 cut(s) 359, 372, 600, 843, 1140, 1170, 1176
Tru9I TTAA 7 cut(s) 359, 372, 600, 843, 1140, 1170, 1176
TscAI CASTG 3 cut(s) 34, 578, 801
TseFI GTSAC 2 cut(s) 193, 352
TseI GCWGC 1 cut(s) 1210
Tsp45I GTSAC 2 cut(s) 193, 352
TspDTI ATGAA 3 cut(s) 204, 513, 1076
TspGWI ACGGA 1 cut(s) 193
TspRI CASTG 3 cut(s) 34, 578, 801
Tth111I GACNNNGTC 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 201
VspI ATTAAT 1 cut(s) 359
XapI RAATTY 1 cut(s) 420
XceI RCATGY 2 cut(s) 990, 1130
XhoI CTCGAG 1 cut(s) 280
XmiI GTMKAC 1 cut(s) 784
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 4 cut(s) 92, 705, 890, 1190
ZrmI AGTACT 1 cut(s) 676
Zsp2I ATGCAT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.