RchiOBHm_Chr2g0093791

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000799
Physical Location & Seq
Reverse (-)
6948954 .. 6952319
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46883

Sequence Viewer

Length: 1236 bp
ATGGCGGCACCTGAAGGTTCACAGTTTGATACACGTCAGTTCGACACCAAGATGAATGAGTTGCTTGCAACTGATGGACAAGATTTCTTTACATCATACGATGAAGTTTACGACAGTTTTGATTCCATGGGCTTGCAAGAGAACCTGCTGAGGGGCATTTATGCTTACGGTTTTGAGAAACCCTCTGCCATTCAGCAAAGGGGAATTGTCCCCTTTATTAAGGGTCTTGATGTGATTCAGCAGGCTCAATCTGGAACTGGGAAGACAGCGACCTTCTGTTCTGGTATTCTTCAACAGCTTGAATATAGTTTGACAGAGTGCCAGGGCTTGGTTCTGGCACCCACTCGAGAGCTTGCCCAACAAATAGAGAAGGTCATGAGGGCCCTTGGAGATTATCTTGGTGTTAAGGTGCATGCATGTGTTGGTGGAACTAGTGTTCGTGAAGACCAACGTATTCTGTCAAATGGAGTTCATGCTGTTGTTGGAACTCCAGGTCGTGTTTTTGATATGCTAAGAAGACAGTCACTTCGCCCTGATCATATCAAGATGTTTGTTCTTGATGAGGCTGATGAAATGCTCTCTCGGGGTTTCAAGGACCAGATCTATGATATATTCCAGCTTCTCCCACCAAAGATTCAGGTTGGTGTGTTTTCTGCCACCATGCCACCTGAGGCCCTGGAGATCACAAGGAAGTTTATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAACTCACCCTGGAAGGAATTAAGCAGTTTTATGTCAATGTGGACAAGGAGGAGTGGAAGCTTGACACTTTGTGTGATCTTTATGAAACCTTGGCCATTACCCAGAGTGTGATTTTCGTGAATACTCGACGCAAGGTTGATTGGTTGACCGACAAAATGAGAAGCCGGGACCATACAGTCTCTGCAACCCATGGAGACATGGACCAGAACAACAGAGACATCATCATGAGGGAATTCCGTTCTGGGTCATCTCGTGTCTTGATCACCACCGATCTGTTGGCTCGTGGTATTGATGTACAACAAGTTTCCCTTGTTATTAACTATGATCTGCCTACCCAGCCTGAGAACTACCTCCATAGAATTGGTCGTAGTGGAAGATTTGGTAGGAAGGGAGTTGCAATCAACTTTGTTACAAAGGATGATGAGAGAATGCTGTTTGATATCCAGAAGTTCTACAATGTGGTAGTAGAGGAGCTGCCCTCAAATGTTGCTGATCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

411

Amino Acids

46.73

Weight (kDa)

5.46

Isoelectric Point (pI)

44.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 63 - 225 4.2e-43 DEAD/DEAH box helicase
ResIII PF04851 81 - 221 6.6e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 264 - 372 8.3e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 908
Acc36I ACCTGC 1 cut(s) 153
AccB1I GGYRCC 2 cut(s) 7, 337
AccB7I CCANNNNNTGG 1 cut(s) 328
AciI CCGC 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 825
AcsI RAATTY 1 cut(s) 965
AcuI CTGAAG 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 804
AfaI GTAC 1 cut(s) 1029
AfiI CCNNNNNNNGG 2 cut(s) 151, 328
AflIII ACRYGT 1 cut(s) 32
AgsI TTSAA 3 cut(s) 293, 302, 592
AhlI ACTAGT 1 cut(s) 431
AjiI CACGTC 1 cut(s) 35
AjnI CCWGG 4 cut(s) 321, 490, 675, 741
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
AluBI AGCT 5 cut(s) 298, 352, 619, 793, 1207
AluI AGCT 5 cut(s) 298, 352, 619, 793, 1207
Alw26I GTCTC 3 cut(s) 916, 921, 942
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 2 cut(s) 345, 582
AoxI GGCC 3 cut(s) 381, 672, 825
ApaI GGGCCC 1 cut(s) 385
ApeKI GCWGC 1 cut(s) 1207
ApoI RAATTY 1 cut(s) 965
ArsI GACNNNNNNTTYG 2 cut(s) 510, 542
AspS9I GGNCC 6 cut(s) 381, 382, 595, 673, 901, 934
AsuC2I CCSGG 1 cut(s) 899
AsuHPI GGTGA 2 cut(s) 730, 988
AvaI CYCGRG 2 cut(s) 345, 582
AvaII GGWCC 3 cut(s) 595, 901, 934
AxyI CCTNAGG 1 cut(s) 669
BaeGI GKGCMC 1 cut(s) 385
BalI TGGCCA 1 cut(s) 827
BanI GGYRCC 2 cut(s) 7, 337
BanII GRGCYC 1 cut(s) 385
BauI CACGAG 2 cut(s) 984, 1014
BbsI GAAGAC 3 cut(s) 269, 450, 523
BbvCI CCTCAGC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 1194
BccI CCATC 1 cut(s) 68
BciT130I CCWGG 4 cut(s) 323, 492, 677, 743
BclI TGATCA 2 cut(s) 535, 993
BcnI CCSGG 1 cut(s) 899
BcoDI GTCTC 3 cut(s) 916, 921, 942
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 1 cut(s) 432
BfuAI ACCTGC 1 cut(s) 153
BglII AGATCT 1 cut(s) 600
BisI GCNGC 2 cut(s) 6, 1208
BlsI GCNGC 2 cut(s) 7, 1209
Bme1390I CCNGG 5 cut(s) 323, 492, 677, 743, 899
Bme18I GGWCC 3 cut(s) 595, 901, 934
BmeT110I CYCGRG 2 cut(s) 345, 582
BmgBI CACGTC 1 cut(s) 35
BmgT120I GGNCC 6 cut(s) 381, 382, 595, 673, 901, 934
BmiI GGNNCC 4 cut(s) 9, 339, 383, 902
BmrFI CCNGG 5 cut(s) 323, 492, 677, 743, 899
BmrI ACTGGG 1 cut(s) 267
BmuI ACTGGG 1 cut(s) 267
BpiI GAAGAC 3 cut(s) 269, 450, 523
BplI GAGNNNNNCTC 4 cut(s) 167, 199, 1196, 1228
BpmI CTGGAG 2 cut(s) 474, 698
Bpu10I CCTNAGC 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 899
BsaJI CCNNGG 7 cut(s) 126, 322, 385, 675, 741, 822, 922
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 328
Bse1I ACTGG 1 cut(s) 262
Bse21I CCTNAGG 1 cut(s) 669
BseBI CCWGG 4 cut(s) 323, 492, 677, 743
BseDI CCNNGG 7 cut(s) 126, 322, 385, 675, 741, 822, 922
BseGI GGATG 1 cut(s) 1156
BseLI CCNNNNNNNGG 2 cut(s) 151, 328
BseMII CTCAG 3 cut(s) 140, 660, 1065
BseNI ACTGG 1 cut(s) 262
BseRI GAGGAG 3 cut(s) 797, 1217, 1220
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 1 cut(s) 1194
BseYI CCCAGC 1 cut(s) 1068
BshFI GGCC 3 cut(s) 383, 674, 827
BshNI GGYRCC 2 cut(s) 7, 337
BsiHKCI CYCGRG 2 cut(s) 345, 582
BsiSI CCGG 1 cut(s) 898
BslFI GGGAC 2 cut(s) 194, 914
BslI CCNNNNNNNGG 2 cut(s) 151, 328
BsmAI GTCTC 3 cut(s) 916, 921, 942
BsmFI GGGAC 2 cut(s) 194, 914
BsmI GAATGC 1 cut(s) 1167
BsnI GGCC 3 cut(s) 383, 674, 827
BsoBI CYCGRG 2 cut(s) 345, 582
Bsp120I GGGCCC 1 cut(s) 381
Bsp1286I GDGCHC 1 cut(s) 385
Bsp1407I TGTACA 1 cut(s) 1027
Bsp143I GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
Bsp19I CCATGG 2 cut(s) 126, 922
BspACI CCGC 1 cut(s) 5
BspANI GGCC 3 cut(s) 383, 674, 827
BspCNI CTCAG 3 cut(s) 141, 661, 1066
BspHI TCATGA 2 cut(s) 375, 957
BspLI GGNNCC 4 cut(s) 9, 339, 383, 902
BspMI ACCTGC 1 cut(s) 153
BspT107I GGYRCC 2 cut(s) 7, 337
BsrGI TGTACA 1 cut(s) 1027
BsrI ACTGG 1 cut(s) 262
BssECI CCNNGG 7 cut(s) 126, 322, 385, 675, 741, 822, 922
BssMI GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
BssSI CACGAG 2 cut(s) 984, 1014
BssT1I CCWWGG 4 cut(s) 126, 385, 822, 922
Bst2BI CACGAG 2 cut(s) 984, 1014
Bst2UI CCWGG 4 cut(s) 323, 492, 677, 743
Bst4CI ACNGT 5 cut(s) 24, 116, 170, 522, 910
BstAUI TGTACA 1 cut(s) 1027
BstC8I GCNNGC 5 cut(s) 66, 134, 243, 354, 414
BstDEI CTNAG 4 cut(s) 149, 512, 669, 1074
BstDSI CCRYGG 2 cut(s) 126, 922
BstF5I GGATG 1 cut(s) 1156
BstKTI GATC 8 cut(s) 538, 603, 684, 811, 996, 1006, 1060, 1228
BstMAI GTCTC 3 cut(s) 916, 921, 942
BstMBI GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
BstMWI GCNNNNNNNGC 1 cut(s) 1069
BstNI CCWGG 4 cut(s) 323, 492, 677, 743
BstNSI RCATGY 2 cut(s) 416, 420
BstSCI CCNGG 5 cut(s) 321, 490, 675, 741, 897
BstSLI GKGCMC 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 1194
BstV2I GAAGAC 3 cut(s) 269, 450, 523
BstX2I RGATCY 1 cut(s) 600
BstXI CCANNNNNNTGG 1 cut(s) 1094
BstYI RGATCY 1 cut(s) 600
Bsu36I CCTNAGG 1 cut(s) 669
BsuRI GGCC 3 cut(s) 383, 674, 827
BtgI CCRYGG 2 cut(s) 126, 922
BtrI CACGTC 1 cut(s) 35
BtsCI GGATG 1 cut(s) 1156
BveI ACCTGC 1 cut(s) 153
Cac8I GCNNGC 5 cut(s) 66, 134, 243, 354, 414
CaiI CAGNNNCTG 1 cut(s) 914
CciI TCATGA 2 cut(s) 375, 957
Cfr13I GGNCC 6 cut(s) 381, 382, 595, 673, 901, 934
CseI GACGC 1 cut(s) 870
Csp6I GTAC 1 cut(s) 1028
CviAII CATG 9 cut(s) 127, 376, 413, 417, 473, 661, 923, 931, 958
CviQI GTAC 1 cut(s) 1028
DdeI CTNAG 4 cut(s) 149, 512, 669, 1074
DpnI GATC 8 cut(s) 537, 602, 683, 810, 995, 1005, 1059, 1227
DpnII GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
DraIII CACNNNGTG 1 cut(s) 804
DrdI GACNNNNNNGTC 1 cut(s) 908
DseDI GACNNNNNNGTC 1 cut(s) 908
EaeI YGGCCR 1 cut(s) 825
Eco130I CCWWGG 4 cut(s) 126, 385, 822, 922
Eco24I GRGCYC 1 cut(s) 385
Eco32I GATATC 1 cut(s) 1174
Eco47I GGWCC 3 cut(s) 595, 901, 934
Eco57I CTGAAG 1 cut(s) 33
Eco81I CCTNAGG 1 cut(s) 669
Eco88I CYCGRG 2 cut(s) 345, 582
EcoO109I RGGNCCY 3 cut(s) 381, 382, 673
EcoRI GAATTC 1 cut(s) 965
EcoRII CCWGG 4 cut(s) 321, 490, 675, 741
EcoRV GATATC 1 cut(s) 1174
EcoT14I CCWWGG 4 cut(s) 126, 385, 822, 922
EcoT22I ATGCAT 1 cut(s) 418
EcoT38I GRGCYC 1 cut(s) 385
ErhI CCWWGG 4 cut(s) 126, 385, 822, 922
FaeI CATG 9 cut(s) 130, 379, 416, 420, 476, 664, 926, 934, 961
FalI AAGNNNNNCTT 2 cut(s) 1026, 1058
FaqI GGGAC 2 cut(s) 194, 914
FatI CATG 9 cut(s) 126, 375, 412, 416, 472, 660, 922, 930, 957
FbaI TGATCA 2 cut(s) 535, 993
Fnu4HI GCNGC 2 cut(s) 6, 1208
FokI GGATG 1 cut(s) 1163
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 2 cut(s) 6, 1208
FspBI CTAG 1 cut(s) 432
GluI GCNGC 2 cut(s) 6, 1208
GsaI CCCAGC 1 cut(s) 1072
GsuI CTGGAG 2 cut(s) 474, 698
HaeIII GGCC 3 cut(s) 383, 674, 827
HapII CCGG 1 cut(s) 898
HgaI GACGC 1 cut(s) 870
Hin1II CATG 9 cut(s) 130, 379, 416, 420, 476, 664, 926, 934, 961
HincII GTYRAC 1 cut(s) 879
HindII GTYRAC 1 cut(s) 879
HindIII AAGCTT 1 cut(s) 791
HinfI GANTC 4 cut(s) 122, 235, 634, 715
HpaII CCGG 1 cut(s) 898
HphI GGTGA 2 cut(s) 730, 988
Hpy166II GTNNAC 4 cut(s) 20, 109, 775, 879
Hpy188I TCNGA 1 cut(s) 1235
Hpy8I GTNNAC 4 cut(s) 20, 109, 775, 879
Hpy99I CGWCG 1 cut(s) 864
HpyAV CCTTC 5 cut(s) 8, 283, 364, 740, 1114
HpyCH4III ACNGT 5 cut(s) 24, 116, 170, 522, 910
HpyCH4IV ACGT 2 cut(s) 34, 451
HpyCH4V TGCA 6 cut(s) 68, 136, 412, 416, 917, 1130
HpyF10VI GCNNNNNNNGC 1 cut(s) 1069
HpyF3I CTNAG 4 cut(s) 149, 512, 669, 1074
HpySE526I ACGT 2 cut(s) 34, 451
Hsp92II CATG 9 cut(s) 130, 379, 416, 420, 476, 664, 926, 934, 961
Ksp22I TGATCA 2 cut(s) 535, 993
Kzo9I GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
LmnI GCTCC 1 cut(s) 1204
Lsp1109I GCAGC 1 cut(s) 1194
MaeI CTAG 1 cut(s) 432
MaeII ACGT 2 cut(s) 34, 451
MaeIII GTNAC 2 cut(s) 522, 1141
MalI GATC 8 cut(s) 537, 602, 683, 810, 995, 1005, 1059, 1227
MboI GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
MboII GAAGA 5 cut(s) 274, 281, 455, 528, 1119
MflI RGATCY 1 cut(s) 600
MhlI GDGCHC 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 827
MluCI AATT 4 cut(s) 204, 750, 965, 1092
MluNI TGGCCA 1 cut(s) 827
MmeI TCCRAC 1 cut(s) 463
Mox20I TGGCCA 1 cut(s) 827
Mph1103I ATGCAT 1 cut(s) 418
MscI TGGCCA 1 cut(s) 827
MseI TTAA 4 cut(s) 219, 405, 753, 1050
MslI CAYNNNNRTG 3 cut(s) 50, 417, 956
Msp20I TGGCCA 1 cut(s) 827
MspI CCGG 1 cut(s) 898
MspR9I CCNGG 5 cut(s) 323, 492, 677, 743, 899
Mva1269I GAATGC 1 cut(s) 1167
MvaI CCWGG 4 cut(s) 323, 492, 677, 743
MwoI GCNNNNNNNGC 1 cut(s) 1069
NciI CCSGG 1 cut(s) 899
NcoI CCATGG 2 cut(s) 126, 922
NdeII GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
NlaIII CATG 9 cut(s) 130, 379, 416, 420, 476, 664, 926, 934, 961
NlaIV GGNNCC 4 cut(s) 9, 339, 383, 902
NmuCI GTSAC 1 cut(s) 522
NsiI ATGCAT 1 cut(s) 418
NspI RCATGY 2 cut(s) 416, 420
PaeI GCATGC 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 345
PagI TCATGA 2 cut(s) 375, 957
PctI GAATGC 1 cut(s) 1167
PfeI GAWTC 4 cut(s) 122, 235, 634, 715
PflMI CCANNNNNTGG 1 cut(s) 328
PkrI GCNGC 2 cut(s) 7, 1209
Psp6I CCWGG 4 cut(s) 321, 490, 675, 741
PspFI CCCAGC 1 cut(s) 1068
PspGI CCWGG 4 cut(s) 321, 490, 675, 741
PspN4I GGNNCC 4 cut(s) 9, 339, 383, 902
PspOMI GGGCCC 1 cut(s) 381
PspPI GGNCC 6 cut(s) 381, 382, 595, 673, 901, 934
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 1 cut(s) 600
RsaI GTAC 1 cut(s) 1029
RsaNI GTAC 1 cut(s) 1028
RseI CAYNNNNRTG 3 cut(s) 50, 417, 956
SaqAI TTAA 4 cut(s) 219, 405, 753, 1050
SatI GCNGC 2 cut(s) 6, 1208
Sau3AI GATC 8 cut(s) 535, 600, 681, 808, 993, 1003, 1057, 1225
Sau96I GGNCC 6 cut(s) 381, 382, 595, 673, 901, 934
ScrFI CCNGG 5 cut(s) 323, 492, 677, 743, 899
SduI GDGCHC 1 cut(s) 385
Sfr274I CTCGAG 1 cut(s) 345
SinI GGWCC 3 cut(s) 595, 901, 934
SlaI CTCGAG 1 cut(s) 345
SmiMI CAYNNNNRTG 3 cut(s) 50, 417, 956
SmlI CTYRAG 1 cut(s) 345
SmoI CTYRAG 1 cut(s) 345
SpeI ACTAGT 1 cut(s) 431
SphI GCATGC 1 cut(s) 416
Sse9I AATT 4 cut(s) 204, 750, 965, 1092
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 5 cut(s) 321, 490, 675, 741, 897
StyI CCWWGG 4 cut(s) 126, 385, 822, 922
TaaI ACNGT 5 cut(s) 24, 116, 170, 522, 910
TaiI ACGT 2 cut(s) 37, 454
TaqI TCGA 3 cut(s) 42, 346, 859
TaqII GACCGA 1 cut(s) 896
TasI AATT 4 cut(s) 204, 750, 965, 1092
TatI WGTACW 1 cut(s) 1027
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 4 cut(s) 122, 235, 634, 715
Tru1I TTAA 4 cut(s) 219, 405, 753, 1050
Tru9I TTAA 4 cut(s) 219, 405, 753, 1050
TseFI GTSAC 1 cut(s) 522
TseI GCWGC 1 cut(s) 1207
Tsp45I GTSAC 1 cut(s) 522
TspDTI ATGAA 7 cut(s) 68, 117, 461, 585, 713, 747, 831
TspGWI ACGGA 1 cut(s) 959
Van91I CCANNNNNTGG 1 cut(s) 328
VpaK11BI GGWCC 3 cut(s) 595, 901, 934
XapI RAATTY 1 cut(s) 965
XceI RCATGY 2 cut(s) 416, 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XhoI CTCGAG 1 cut(s) 345
XspI CTAG 1 cut(s) 432
Zsp2I ATGCAT 1 cut(s) 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.