Rh2DG084700

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000799
Physical Location & Seq
Reverse (-)
6605422 .. 6605997
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG084700.1

Sequence Viewer

Length: 576 bp
ATGCCACCTGAGGCCCTGGAGATCACAAGGAAGTTTATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAACTCACCCTGGAAGGAATTAAGCAGTTTTATGTCAATGTGGACAAGGAGGAGTGGAAGCTTGACACTTTGTGTGATCTTTATGAAACCTTGGCCATTACCCAGAGTGTGATTTTCGTGAATACTCGACGCAAGGTTGATTGGTTGACCGACAAAATGAGAAGCCGGGACCATACAGTCTCTGCAACCCATGGAGACATGGACCAGAACAACAGAGACATCATCATGAGGGAATTCCGTTCTGGGTCATCTCGTGTCTTGATCACCACCGATCTGTTGGCTCGTGGTATTGATGTACAACAAGTTTCCCTTGTTATTAACTATGATCTGCCTACCCAGCCTGAGAACTACCTCCATAGAATTGGTCGTAGTGGAAGATTTGGTAGGAAGGGAGTTGCAATCAACTTTGTTACAAAGGATGATGAGAGAATGCTGTTTGATATCCAGAAGTTCTACAATGTGGTAGTAGAGGAGCTGCCCTCAAATGTTGCTGATCTCCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

191

Amino Acids

22.26

Weight (kDa)

6.33

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 44 - 152 1.5e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 305
AdeI CACNNNGTG 1 cut(s) 144
AfaI GTAC 1 cut(s) 369
AjnI CCWGG 2 cut(s) 15, 81
AluBI AGCT 2 cut(s) 133, 547
AluI AGCT 2 cut(s) 133, 547
Alw26I GTCTC 3 cut(s) 256, 261, 282
AlwNI CAGNNNCTG 1 cut(s) 254
AoxI GGCC 2 cut(s) 12, 165
ApeKI GCWGC 1 cut(s) 547
ApoI RAATTY 1 cut(s) 305
AspS9I GGNCC 3 cut(s) 13, 241, 274
AsuC2I CCSGG 1 cut(s) 239
AsuHPI GGTGA 2 cut(s) 70, 328
AvaII GGWCC 2 cut(s) 241, 274
AxyI CCTNAGG 1 cut(s) 9
BalI TGGCCA 1 cut(s) 167
BauI CACGAG 2 cut(s) 324, 354
BbvI GCAGC 1 cut(s) 534
BciT130I CCWGG 2 cut(s) 17, 83
BclI TGATCA 1 cut(s) 333
BcnI CCSGG 1 cut(s) 239
BcoDI GTCTC 3 cut(s) 256, 261, 282
BisI GCNGC 1 cut(s) 548
BlsI GCNGC 1 cut(s) 549
Bme1390I CCNGG 3 cut(s) 17, 83, 239
Bme18I GGWCC 2 cut(s) 241, 274
BmgT120I GGNCC 3 cut(s) 13, 241, 274
BmiI GGNNCC 1 cut(s) 242
BmrFI CCNGG 3 cut(s) 17, 83, 239
BplI GAGNNNNNCTC 2 cut(s) 536, 568
BpmI CTGGAG 1 cut(s) 38
BpuMI CCSGG 1 cut(s) 239
BsaJI CCNNGG 4 cut(s) 15, 81, 162, 262
Bse21I CCTNAGG 1 cut(s) 9
BseBI CCWGG 2 cut(s) 17, 83
BseDI CCNNGG 4 cut(s) 15, 81, 162, 262
BseGI GGATG 1 cut(s) 496
BseMII CTCAG 1 cut(s) 405
BseRI GAGGAG 3 cut(s) 137, 557, 560
BseXI GCAGC 1 cut(s) 534
BseYI CCCAGC 1 cut(s) 408
BshFI GGCC 2 cut(s) 14, 167
BsiSI CCGG 1 cut(s) 238
BslFI GGGAC 1 cut(s) 254
BsmAI GTCTC 3 cut(s) 256, 261, 282
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 507
BsnI GGCC 2 cut(s) 14, 167
Bsp1407I TGTACA 1 cut(s) 367
Bsp143I GATC 6 cut(s) 21, 148, 333, 343, 397, 565
Bsp19I CCATGG 1 cut(s) 262
BspANI GGCC 2 cut(s) 14, 167
BspCNI CTCAG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 367
BssECI CCNNGG 4 cut(s) 15, 81, 162, 262
BssMI GATC 6 cut(s) 21, 148, 333, 343, 397, 565
BssSI CACGAG 2 cut(s) 324, 354
BssT1I CCWWGG 2 cut(s) 162, 262
Bst2BI CACGAG 2 cut(s) 324, 354
Bst2UI CCWGG 2 cut(s) 17, 83
Bst4CI ACNGT 1 cut(s) 250
BstAUI TGTACA 1 cut(s) 367
BstDEI CTNAG 2 cut(s) 9, 414
BstDSI CCRYGG 1 cut(s) 262
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 6 cut(s) 24, 151, 336, 346, 400, 568
BstMAI GTCTC 3 cut(s) 256, 261, 282
BstMBI GATC 6 cut(s) 21, 148, 333, 343, 397, 565
BstMWI GCNNNNNNNGC 1 cut(s) 409
BstNI CCWGG 2 cut(s) 17, 83
BstSCI CCNGG 3 cut(s) 15, 81, 237
BstV1I GCAGC 1 cut(s) 534
BstXI CCANNNNNNTGG 1 cut(s) 434
Bsu36I CCTNAGG 1 cut(s) 9
BsuRI GGCC 2 cut(s) 14, 167
BtgI CCRYGG 1 cut(s) 262
BtsCI GGATG 1 cut(s) 496
CaiI CAGNNNCTG 1 cut(s) 254
CciI TCATGA 1 cut(s) 297
Cfr13I GGNCC 3 cut(s) 13, 241, 274
CseI GACGC 1 cut(s) 210
Csp6I GTAC 1 cut(s) 368
CviAII CATG 3 cut(s) 263, 271, 298
CviJI RGCY 7 cut(s) 14, 133, 167, 237, 353, 412, 547
CviKI_1 RGCY 7 cut(s) 14, 133, 167, 237, 353, 412, 547
CviQI GTAC 1 cut(s) 368
DdeI CTNAG 2 cut(s) 9, 414
DpnI GATC 6 cut(s) 23, 150, 335, 345, 399, 567
DpnII GATC 6 cut(s) 21, 148, 333, 343, 397, 565
DraIII CACNNNGTG 1 cut(s) 144
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
EaeI YGGCCR 1 cut(s) 165
Eco130I CCWWGG 2 cut(s) 162, 262
Eco32I GATATC 1 cut(s) 514
Eco47I GGWCC 2 cut(s) 241, 274
Eco81I CCTNAGG 1 cut(s) 9
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 15, 81
EcoRV GATATC 1 cut(s) 514
EcoT14I CCWWGG 2 cut(s) 162, 262
ErhI CCWWGG 2 cut(s) 162, 262
FaeI CATG 3 cut(s) 266, 274, 301
FaiI YATR 9 cut(s) 38, 105, 156, 246, 264, 272, 299, 396, 429
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 1 cut(s) 254
FatI CATG 3 cut(s) 262, 270, 297
FbaI TGATCA 1 cut(s) 333
Fnu4HI GCNGC 1 cut(s) 548
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 1 cut(s) 548
GluI GCNGC 1 cut(s) 548
GsaI CCCAGC 1 cut(s) 412
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 2 cut(s) 14, 167
HapII CCGG 1 cut(s) 238
HgaI GACGC 1 cut(s) 210
Hin1II CATG 3 cut(s) 266, 274, 301
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 1 cut(s) 55
HpaII CCGG 1 cut(s) 238
HphI GGTGA 2 cut(s) 70, 328
Hpy166II GTNNAC 2 cut(s) 115, 219
Hpy188I TCNGA 1 cut(s) 575
Hpy188III TCNNGA 4 cut(s) 190, 298, 331, 517
Hpy8I GTNNAC 2 cut(s) 115, 219
Hpy99I CGWCG 1 cut(s) 204
HpyAV CCTTC 2 cut(s) 80, 454
HpyCH4III ACNGT 1 cut(s) 250
HpyCH4V TGCA 2 cut(s) 257, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 409
HpyF3I CTNAG 2 cut(s) 9, 414
Hsp92II CATG 3 cut(s) 266, 274, 301
Ksp22I TGATCA 1 cut(s) 333
Kzo9I GATC 6 cut(s) 21, 148, 333, 343, 397, 565
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 1 cut(s) 534
MaeIII GTNAC 1 cut(s) 481
MalI GATC 6 cut(s) 23, 150, 335, 345, 399, 567
MboI GATC 6 cut(s) 21, 148, 333, 343, 397, 565
MboII GAAGA 1 cut(s) 459
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 3 cut(s) 90, 305, 432
MluNI TGGCCA 1 cut(s) 167
MnlI CCTC 7 cut(s) 4, 45, 115, 294, 434, 535, 562
Mox20I TGGCCA 1 cut(s) 167
MscI TGGCCA 1 cut(s) 167
MseI TTAA 2 cut(s) 93, 390
MslI CAYNNNNRTG 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 167
MspI CCGG 1 cut(s) 238
MspR9I CCNGG 3 cut(s) 17, 83, 239
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 2 cut(s) 17, 83
MwoI GCNNNNNNNGC 1 cut(s) 409
NciI CCSGG 1 cut(s) 239
NcoI CCATGG 1 cut(s) 262
NdeII GATC 6 cut(s) 21, 148, 333, 343, 397, 565
NlaIII CATG 3 cut(s) 266, 274, 301
NlaIV GGNNCC 1 cut(s) 242
PagI TCATGA 1 cut(s) 297
PctI GAATGC 1 cut(s) 507
PfeI GAWTC 1 cut(s) 55
PkrI GCNGC 1 cut(s) 549
Psp6I CCWGG 2 cut(s) 15, 81
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 2 cut(s) 15, 81
PspN4I GGNNCC 1 cut(s) 242
PspPI GGNCC 3 cut(s) 13, 241, 274
PstNI CAGNNNCTG 1 cut(s) 254
RsaI GTAC 1 cut(s) 369
RsaNI GTAC 1 cut(s) 368
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 2 cut(s) 93, 390
SatI GCNGC 1 cut(s) 548
Sau3AI GATC 6 cut(s) 21, 148, 333, 343, 397, 565
Sau96I GGNCC 3 cut(s) 13, 241, 274
ScrFI CCNGG 3 cut(s) 17, 83, 239
SetI ASST 7 cut(s) 10, 49, 135, 164, 210, 426, 549
SinI GGWCC 2 cut(s) 241, 274
SmiMI CAYNNNNRTG 1 cut(s) 296
Sse9I AATT 3 cut(s) 90, 305, 432
StyD4I CCNGG 3 cut(s) 15, 81, 237
StyI CCWWGG 2 cut(s) 162, 262
TaaI ACNGT 1 cut(s) 250
TaqI TCGA 1 cut(s) 199
TaqII GACCGA 1 cut(s) 236
TasI AATT 3 cut(s) 90, 305, 432
TatI WGTACW 1 cut(s) 367
TfiI GAWTC 1 cut(s) 55
Tru1I TTAA 2 cut(s) 93, 390
Tru9I TTAA 2 cut(s) 93, 390
TseI GCWGC 1 cut(s) 547
TspDTI ATGAA 3 cut(s) 53, 87, 171
TspGWI ACGGA 1 cut(s) 299
VpaK11BI GGWCC 2 cut(s) 241, 274
XapI RAATTY 1 cut(s) 305
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.