RLG00000010423

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000799
Physical Location & Seq
Forward (+)
1524460 .. 1528991
4532 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010423

Sequence Viewer

Length: 1305 bp
ATGAATACAGTCTTTATGTCACCGACTCTCACTTCTCCGAGCTCTCAACTACCTCTTTCAGTCATGGCTGGTGCTGCACCGGAAGGATCTCAATTTGACGCTCGTCAATATGATGCGAAGATGAACGAGCTACTTGGATCTGATGGACAAGACTTCTTCACCTCGTATGATGAGGTTCATGACAGTTTTGATGTCATGGGACTGCAGGAGAACCTTCTCAGGGGCATCTATGCATATGGTTTTGAGAAGCCCTCTGCAATTCAGCAAAGGGGAATTGTTCCCTTCTGCAAGGGACTTGATGTGATTCAACAGGCACAATCTGGAACTGGAAAAACAGCCACTTTCTGCTCTGGAATTTTACAGCAGCTTGATTATGGCCTACTCGATTGTCAGGCACTGGTTCTTGCACCTACCCGTGAACTTGCTCAACAGATTGAGAAGGTTATGCGGGCTCTGGGTGACTATCTCGGTGTCAAAGTTCATGCTTGTGTTGGCGGGACCAGTGTTCGTGAAGATCAACGTGTCCTCTCTAGTGGAGTCCATGTTGTTGTTGGTACGCCTGGTCGAGTATTTGACATGTTGCGGAGACAGTCTCTCCGTCCAGATTGCATTAGGATGTTTGTGTTAGATGAAGCTGATGAAATGCTCTCACGAGGTTTCAAGGATCAGATCTATGACATTTTCCAGCTGCTACCACCCAAAATTCAGGTGGGAGTTTTTTCTGCCACAATGCCACCAGAAGCCCTTGATATCACCAGGAAGTTCATGAACAAACCTGTGAGGATTTTGGTGAAACGTGATGAGCTCACTCTTGAGGGTATCAAGCAGTTCCATGTCAATGTTGACAAGGAGGAGTGGAAACTTGAGACGCTTTGCGATCTTTATGAGACCTTGGCAATCACCCAGAGTGTCATCTTTGTGAACACCAGGCGCAAAGTTGATTGGCTGACAGACAAGATGCGCAGCCGTGATCACACGGTCTCTGCCACCCATGGTGACATGGAGCAAAACACTAGGGACATCATCATGAGAGAGTTTAGGTCTGGTTCTTCTCGTGTGCTCATAACAACTGATCTGTTGGCCCGTGGTATTGATGTCCAGCAGGTCTCTCTTGTGATTAACTATGACCTTCCAACACAGCCGGAAAACTACCTCCATCGTATTGGTCGTAGTGGTAGGTTTGGGAGGAAGGGTGTTGCCATCAACTTTGTGACCAAGGATGATGAAAGGATGTTGTTCGACATCCAGAGGTTCTACAATGTTGTAATTGAGGAGCTGCCGGCTAATGTTGCTGATCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

435

Amino Acids

48.93

Weight (kDa)

5.53

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 86 - 248 2.5e-42 DEAD/DEAH box helicase
ResIII PF04851 104 - 244 7.2e-06 Type III restriction enzyme, res subunit
Helicase_C PF00271 287 - 395 7.5e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 962
Acc36I ACCTGC 1 cut(s) 1093
AciI CCGC 3 cut(s) 448, 495, 583
AclWI GGATC 3 cut(s) 94, 145, 672
AcsI RAATTY 2 cut(s) 354, 702
AfaI GTAC 1 cut(s) 556
AfiI CCNNNNNNNGG 1 cut(s) 220
AflIII ACRYGT 2 cut(s) 520, 576
AgsI TTSAA 2 cut(s) 308, 661
AjnI CCWGG 3 cut(s) 559, 755, 926
AluBI AGCT 7 cut(s) 42, 130, 367, 635, 688, 805, 1276
AluI AGCT 7 cut(s) 42, 130, 367, 635, 688, 805, 1276
Alw21I GWGCWC 3 cut(s) 44, 807, 1062
Alw26I GTCTC 6 cut(s) 580, 597, 860, 881, 985, 1111
AlwI GGATC 3 cut(s) 94, 145, 672
AlwNI CAGNNNCTG 1 cut(s) 397
AoxI GGCC 2 cut(s) 376, 1080
ApeKI GCWGC 5 cut(s) 74, 364, 688, 963, 1276
ApoI RAATTY 2 cut(s) 354, 702
AspLEI GCGC 2 cut(s) 933, 963
AspS9I GGNCC 2 cut(s) 498, 1081
AsuHPI GGTGA 7 cut(s) 12, 151, 470, 745, 802, 892, 1007
AvaII GGWCC 1 cut(s) 498
BanII GRGCYC 3 cut(s) 44, 454, 807
BauI CACGAG 2 cut(s) 651, 1053
Bbv12I GWGCWC 3 cut(s) 44, 807, 1062
BbvI GCAGC 5 cut(s) 61, 376, 675, 975, 1263
BccI CCATC 3 cut(s) 137, 1164, 1208
BceAI ACGGC 1 cut(s) 951
BciT130I CCWGG 3 cut(s) 561, 757, 928
BclI TGATCA 1 cut(s) 970
BcoDI GTCTC 6 cut(s) 580, 597, 860, 881, 985, 1111
BfaI CTAG 2 cut(s) 531, 1014
BfmI CTRYAG 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 1093
BglII AGATCT 1 cut(s) 669
BisI GCNGC 5 cut(s) 75, 365, 689, 964, 1277
BlsI GCNGC 5 cut(s) 76, 366, 690, 965, 1278
Bme1390I CCNGG 3 cut(s) 561, 757, 928
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 498, 1081
BmiI GGNNCC 1 cut(s) 499
BmrFI CCNGG 3 cut(s) 561, 757, 928
BmsI GCATC 3 cut(s) 103, 234, 948
BoxI GACNNNNGTC 1 cut(s) 102
BplI GAGNNNNNCTC 4 cut(s) 236, 268, 577, 609
BpuEI CTTGAG 2 cut(s) 833, 884
BsaI GGTCTC 3 cut(s) 881, 985, 1111
BsaJI CCNNGG 4 cut(s) 891, 991, 1084, 1215
BsaWI WCCGGW 1 cut(s) 79
BsaXI ACNNNNNCTCC 6 cut(s) 528, 558, 579, 609, 1177, 1207
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse118I RCCGGY 1 cut(s) 1279
Bse1I ACTGG 3 cut(s) 331, 402, 501
BseBI CCWGG 3 cut(s) 561, 757, 928
BseDI CCNNGG 4 cut(s) 891, 991, 1084, 1215
BseGI GGATG 4 cut(s) 621, 1225, 1236, 1242
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMII CTCAG 1 cut(s) 232
BseNI ACTGG 3 cut(s) 331, 402, 501
BseRI GAGGAG 2 cut(s) 866, 1286
BseXI GCAGC 5 cut(s) 61, 376, 675, 975, 1263
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 2 cut(s) 378, 1082
BsiHKAI GWGCWC 3 cut(s) 44, 807, 1062
BsiSI CCGG 3 cut(s) 80, 1142, 1280
BslFI GGGAC 4 cut(s) 213, 306, 511, 1031
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 6 cut(s) 580, 597, 860, 881, 985, 1111
BsmBI CGTCTC 1 cut(s) 860
BsmFI GGGAC 4 cut(s) 213, 306, 511, 1031
BsnI GGCC 2 cut(s) 378, 1082
Bso31I GGTCTC 3 cut(s) 881, 985, 1111
Bsp1286I GDGCHC 4 cut(s) 44, 454, 807, 1062
Bsp143I GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
Bsp19I CCATGG 1 cut(s) 991
BspACI CCGC 3 cut(s) 448, 495, 583
BspANI GGCC 2 cut(s) 378, 1082
BspCNI CTCAG 1 cut(s) 231
BspHI TCATGA 3 cut(s) 178, 765, 1026
BspLI GGNNCC 1 cut(s) 499
BspMAI CTGCAG 1 cut(s) 207
BspMI ACCTGC 1 cut(s) 1093
BspPI GGATC 3 cut(s) 94, 145, 672
BspTNI GGTCTC 3 cut(s) 881, 985, 1111
BsrFI RCCGGY 1 cut(s) 1279
BsrI ACTGG 3 cut(s) 331, 402, 501
BssAI RCCGGY 1 cut(s) 1279
BssECI CCNNGG 4 cut(s) 891, 991, 1084, 1215
BssMI GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
BssSI CACGAG 2 cut(s) 651, 1053
BssT1I CCWWGG 3 cut(s) 891, 991, 1215
Bst2BI CACGAG 2 cut(s) 651, 1053
Bst2UI CCWGG 3 cut(s) 561, 757, 928
Bst4CI ACNGT 4 cut(s) 10, 185, 591, 979
BstC8I GCNNGC 2 cut(s) 450, 1281
BstDEI CTNAG 1 cut(s) 218
BstDSI CCRYGG 2 cut(s) 991, 1084
BstF5I GGATG 4 cut(s) 621, 1225, 1236, 1242
BstHHI GCGC 2 cut(s) 933, 963
BstKTI GATC 9 cut(s) 89, 140, 517, 667, 672, 880, 973, 1075, 1297
BstMAI GTCTC 6 cut(s) 580, 597, 860, 881, 985, 1111
BstMBI GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
BstMWI GCNNNNNNNGC 2 cut(s) 74, 1289
BstNI CCWGG 3 cut(s) 561, 757, 928
BstNSI RCATGY 1 cut(s) 580
BstPAI GACNNNNGTC 1 cut(s) 102
BstSCI CCNGG 3 cut(s) 559, 755, 926
BstSFI CTRYAG 1 cut(s) 203
BstV1I GCAGC 5 cut(s) 61, 376, 675, 975, 1263
BstX2I RGATCY 3 cut(s) 86, 137, 669
BstXI CCANNNNNNTGG 1 cut(s) 1163
BstYI RGATCY 3 cut(s) 86, 137, 669
BsuRI GGCC 2 cut(s) 378, 1082
BtgI CCRYGG 2 cut(s) 991, 1084
BtsCI GGATG 4 cut(s) 621, 1225, 1236, 1242
BtsIMutI CAGTG 2 cut(s) 395, 508
BveI ACCTGC 1 cut(s) 1093
Cac8I GCNNGC 2 cut(s) 450, 1281
CaiI CAGNNNCTG 1 cut(s) 397
CciI TCATGA 3 cut(s) 178, 765, 1026
CfoI GCGC 2 cut(s) 933, 963
Cfr10I RCCGGY 1 cut(s) 1279
Cfr13I GGNCC 2 cut(s) 498, 1081
CseI GACGC 2 cut(s) 107, 877
Csp6I GTAC 1 cut(s) 555
CviQI GTAC 1 cut(s) 555
DdeI CTNAG 1 cut(s) 218
DpnI GATC 9 cut(s) 88, 139, 516, 666, 671, 879, 972, 1074, 1296
DpnII GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
Ecl136II GAGCTC 2 cut(s) 42, 805
Eco130I CCWWGG 3 cut(s) 891, 991, 1215
Eco24I GRGCYC 3 cut(s) 44, 454, 807
Eco31I GGTCTC 3 cut(s) 881, 985, 1111
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 498
Eco53kI GAGCTC 2 cut(s) 42, 805
EcoICRI GAGCTC 2 cut(s) 42, 805
EcoRII CCWGG 3 cut(s) 559, 755, 926
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 3 cut(s) 891, 991, 1215
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 3 cut(s) 44, 454, 807
ErhI CCWWGG 3 cut(s) 891, 991, 1215
Esp3I CGTCTC 1 cut(s) 860
FaqI GGGAC 4 cut(s) 213, 306, 511, 1031
FauI CCCGC 2 cut(s) 441, 488
FauNDI CATATG 1 cut(s) 235
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 5 cut(s) 75, 365, 689, 964, 1277
FokI GGATG 4 cut(s) 628, 1229, 1232, 1243
FriOI GRGCYC 3 cut(s) 44, 454, 807
Fsp4HI GCNGC 5 cut(s) 75, 365, 689, 964, 1277
FspBI CTAG 2 cut(s) 531, 1014
FspI TGCGCA 1 cut(s) 962
GlaI GCGC 2 cut(s) 932, 962
GluI GCNGC 5 cut(s) 75, 365, 689, 964, 1277
HaeIII GGCC 2 cut(s) 378, 1082
HapII CCGG 3 cut(s) 80, 1142, 1280
HgaI GACGC 2 cut(s) 107, 877
HhaI GCGC 2 cut(s) 933, 963
Hin6I GCGC 2 cut(s) 931, 961
HinP1I GCGC 2 cut(s) 931, 961
HincII GTYRAC 1 cut(s) 844
HindII GTYRAC 1 cut(s) 844
HinfI GANTC 3 cut(s) 25, 304, 537
HpaII CCGG 3 cut(s) 80, 1142, 1280
HphI GGTGA 7 cut(s) 12, 151, 470, 745, 802, 892, 1007
Hpy166II GTNNAC 3 cut(s) 419, 844, 922
Hpy188I TCNGA 3 cut(s) 39, 142, 669
Hpy8I GTNNAC 3 cut(s) 419, 844, 922
HpyAV CCTTC 6 cut(s) 77, 224, 292, 433, 1139, 1183
HpyCH4III ACNGT 4 cut(s) 10, 185, 591, 979
HpyCH4IV ACGT 2 cut(s) 520, 796
HpyCH4V TGCA 7 cut(s) 77, 205, 233, 257, 288, 407, 609
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 1289
HpyF3I CTNAG 1 cut(s) 218
HpySE526I ACGT 2 cut(s) 520, 796
HspAI GCGC 2 cut(s) 931, 961
KroI GCCGGC 1 cut(s) 1279
KroNI GCCGGC 1 cut(s) 1281
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
LmnI GCTCC 2 cut(s) 1003, 1273
Lsp1109I GCAGC 5 cut(s) 61, 376, 675, 975, 1263
LweI GCATC 3 cut(s) 103, 234, 948
MaeI CTAG 2 cut(s) 531, 1014
MaeII ACGT 2 cut(s) 520, 796
MaeIII GTNAC 4 cut(s) 18, 458, 995, 1210
MalI GATC 9 cut(s) 88, 139, 516, 666, 671, 879, 972, 1074, 1296
MboI GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
MboII GAAGA 5 cut(s) 130, 148, 524, 1041, 1289
MflI RGATCY 3 cut(s) 86, 137, 669
MhlI GDGCHC 4 cut(s) 44, 454, 807, 1062
MluCI AATT 6 cut(s) 92, 258, 273, 354, 702, 1266
MlyI GAGTC 2 cut(s) 19, 546
MmeI TCCRAC 1 cut(s) 1157
Mph1103I ATGCAT 1 cut(s) 235
MroNI GCCGGC 1 cut(s) 1279
MseI TTAA 2 cut(s) 1119, 1303
MslI CAYNNNNRTG 5 cut(s) 486, 614, 837, 917, 1025
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 3 cut(s) 80, 1142, 1280
MspR9I CCNGG 3 cut(s) 561, 757, 928
MvaI CCWGG 3 cut(s) 561, 757, 928
MwoI GCNNNNNNNGC 2 cut(s) 74, 1289
NaeI GCCGGC 1 cut(s) 1281
NcoI CCATGG 1 cut(s) 991
NdeI CATATG 1 cut(s) 235
NdeII GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
NgoMIV GCCGGC 1 cut(s) 1279
NlaIV GGNNCC 1 cut(s) 499
NmuCI GTSAC 4 cut(s) 18, 458, 995, 1210
NsbI TGCGCA 1 cut(s) 962
NsiI ATGCAT 1 cut(s) 235
NspI RCATGY 1 cut(s) 580
PagI TCATGA 3 cut(s) 178, 765, 1026
PciI ACATGT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 1165
PdiI GCCGGC 1 cut(s) 1281
PfeI GAWTC 1 cut(s) 304
PkrI GCNGC 5 cut(s) 76, 366, 690, 965, 1278
PleI GAGTC 2 cut(s) 19, 545
PpsI GAGTC 2 cut(s) 19, 545
PscI ACATGT 1 cut(s) 576
PshAI GACNNNNGTC 1 cut(s) 102
Psp124BI GAGCTC 2 cut(s) 44, 807
Psp6I CCWGG 3 cut(s) 559, 755, 926
PspGI CCWGG 3 cut(s) 559, 755, 926
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 2 cut(s) 498, 1081
PstI CTGCAG 1 cut(s) 207
PstNI CAGNNNCTG 1 cut(s) 397
PsuI RGATCY 3 cut(s) 86, 137, 669
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 1 cut(s) 556
RsaNI GTAC 1 cut(s) 555
RseI CAYNNNNRTG 5 cut(s) 486, 614, 837, 917, 1025
SacI GAGCTC 2 cut(s) 44, 807
SaqAI TTAA 2 cut(s) 1119, 1303
SatI GCNGC 5 cut(s) 75, 365, 689, 964, 1277
Sau3AI GATC 9 cut(s) 86, 137, 514, 664, 669, 877, 970, 1072, 1294
Sau96I GGNCC 2 cut(s) 498, 1081
SchI GAGTC 2 cut(s) 19, 546
ScrFI CCNGG 3 cut(s) 561, 757, 928
SduI GDGCHC 4 cut(s) 44, 454, 807, 1062
SfaNI GCATC 3 cut(s) 103, 234, 948
SfcI CTRYAG 1 cut(s) 203
SinI GGWCC 1 cut(s) 498
SmiMI CAYNNNNRTG 5 cut(s) 486, 614, 837, 917, 1025
SmlI CTYRAG 2 cut(s) 812, 863
SmoI CTYRAG 2 cut(s) 812, 863
Sse9I AATT 6 cut(s) 92, 258, 273, 354, 702, 1266
SsiI CCGC 3 cut(s) 448, 495, 583
SspMI CTAG 2 cut(s) 531, 1014
SstI GAGCTC 2 cut(s) 44, 807
StyD4I CCNGG 3 cut(s) 559, 755, 926
StyI CCWWGG 3 cut(s) 891, 991, 1215
TaaI ACNGT 4 cut(s) 10, 185, 591, 979
TaiI ACGT 2 cut(s) 523, 799
TaqI TCGA 3 cut(s) 384, 565, 1239
TasI AATT 6 cut(s) 92, 258, 273, 354, 702, 1266
TfiI GAWTC 1 cut(s) 304
Tru1I TTAA 2 cut(s) 1119, 1303
Tru9I TTAA 2 cut(s) 1119, 1303
TscAI CASTG 2 cut(s) 402, 508
TseFI GTSAC 4 cut(s) 18, 458, 995, 1210
TseI GCWGC 5 cut(s) 74, 364, 688, 963, 1276
Tsp45I GTSAC 4 cut(s) 18, 458, 995, 1210
TspDTI ATGAA 9 cut(s) 17, 137, 167, 470, 645, 654, 754, 782, 1239
TspGWI ACGGA 1 cut(s) 587
TspRI CASTG 2 cut(s) 402, 508
VpaK11BI GGWCC 1 cut(s) 498
XapI RAATTY 2 cut(s) 354, 702
XceI RCATGY 1 cut(s) 580
XcmI CCANNNNNNNNNTGG 2 cut(s) 548, 706
XspI CTAG 2 cut(s) 531, 1014
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.