Rmu_co8454423.1_g000001

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8454423.1
OG0000799
Physical Location & Seq
Reverse (-)
2 .. 1547
1546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8454423.1_g000001.1.cds

Sequence Viewer

Length: 1171 bp
atggcaggtcttgctcccgaaggtgcacagtttgatgctaaacagtatgattctaagatgaatgagatcctctcatctgaaggtcaagagttcttcacatcgtatgatgaggttcatgacagttttgatgccatgggtttacaagaaaatcttttaaggggaatatatgcttatggatttgagaagccttctgcgattcagcaaagaggaattgttcctttctgtaaaggtttggatgtgattcaacaagctcagtcaggaacaggcaaaacagcaaccttttgctctggaattttgcagcaacttgactatggtttggtccagtgccaggctttggtgttggcacctaccagggagttagctcagcagattgagaaggttatgcgggcacttggagactatctcggcgtgaaggttcatgcttgtgtaggtgggactagtgttcgtgaggatcagcgaattcttcagactggagtgcatgtggtggttggcactcctggacgtgtgtttgacatgttgcggaggcaatctcttcgtgcagattacattaagatgtttgtattggatgaagccgatgaaatgctatctaggggtttcaaagatcagatctatgacatttttcagcttctgccttcaaaagttcaagttggtgtgttctctgctacaatgcctcctgaagcccttgagatcaccagaaagtttatgaacaaacctgttaggattttggttaagcgtgatgagctcaccctggagggaatcaagcaattttatgttaatgtcgataaggaagaatggaagctggagacactctgcgatctctatgagacccttgcaatcactcagagtgtcatttttgttaatacaaggcgcaaagtggattggcttacagataagatgcgaagccgtgaccacactgtgtctgctacccatggtgatatggaccagaaccagcgtgacattatcatgcgtgaattccgatctggttcctcccgtgttctcatcactactgatctgttggcacgtggtattgatgtgcagcaagtgtcgcttgtaattaattatgatttgccgactcaaccagaaaactaccttcatcgtatcggacgaagtgggcgatttggaagaaaaggtgttgccatcaatttcgtgactagggatgacgatagaatgc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

390

Amino Acids

44.2

Weight (kDa)

5.96

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 343
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 2 cut(s) 385, 520
AclWI GGATC 2 cut(s) 61, 459
AcsI RAATTY 3 cut(s) 291, 459, 971
AcuI CTGAAG 3 cut(s) 99, 449, 696
AcvI CACGTG 1 cut(s) 1022
AdeI CACNNNGTG 1 cut(s) 916
AfiI CCNNNNNNNGG 2 cut(s) 328, 334
AflIII ACRYGT 2 cut(s) 502, 513
AgsI TTSAA 4 cut(s) 245, 598, 636, 644
AhlI ACTAGT 1 cut(s) 437
AjiI CACGTC 1 cut(s) 503
AjnI CCWGG 4 cut(s) 327, 350, 496, 747
AluBI AGCT 5 cut(s) 251, 362, 625, 742, 799
AluI AGCT 5 cut(s) 251, 362, 625, 742, 799
Alw21I GWGCWC 2 cut(s) 28, 744
Alw26I GTCTC 3 cut(s) 390, 797, 818
Alw44I GTGCAC 1 cut(s) 24
AlwI GGATC 2 cut(s) 61, 459
AlwNI CAGNNNCTG 1 cut(s) 628
ApaLI GTGCAC 1 cut(s) 24
ApeKI GCWGC 2 cut(s) 298, 1036
ApoI RAATTY 3 cut(s) 291, 459, 971
AseI ATTAAT 1 cut(s) 1056
AspLEI GCGC 1 cut(s) 870
AspS9I GGNCC 2 cut(s) 319, 940
AsuHPI GGTGA 3 cut(s) 682, 736, 944
AvaII GGWCC 2 cut(s) 319, 940
BaeGI GKGCMC 2 cut(s) 28, 391
BanI GGYRCC 1 cut(s) 343
BanII GRGCYC 1 cut(s) 744
BbrPI CACGTG 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 28, 744
BbvI GCAGC 2 cut(s) 310, 1048
BccI CCATC 1 cut(s) 1145
BceAI ACGGC 1 cut(s) 888
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 4 cut(s) 329, 352, 498, 749
BcoDI GTCTC 3 cut(s) 390, 797, 818
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 3 cut(s) 438, 588, 1152
BglII AGATCT 1 cut(s) 606
BisI GCNGC 2 cut(s) 299, 1037
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 2 cut(s) 300, 1038
Bme1390I CCNGG 4 cut(s) 329, 352, 498, 749
Bme18I GGWCC 2 cut(s) 319, 940
BmgBI CACGTC 1 cut(s) 503
BmgT120I GGNCC 2 cut(s) 319, 940
BmiI GGNNCC 2 cut(s) 345, 985
BmrFI CCNGG 4 cut(s) 329, 352, 498, 749
BmsI GCATC 3 cut(s) 25, 118, 885
BplI GAGNNNNNCTC 6 cut(s) 56, 88, 387, 419, 514, 546
BpmI CTGGAG 3 cut(s) 492, 770, 821
Bpu1102I GCTNAGC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 704
BsaAI YACGTR 1 cut(s) 1022
BsaI GGTCTC 1 cut(s) 818
BsaJI CCNNGG 4 cut(s) 132, 351, 747, 928
BsaXI ACNNNNNCTCC 4 cut(s) 465, 495, 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 328, 334
Bse1I ACTGG 2 cut(s) 322, 475
BseBI CCWGG 4 cut(s) 329, 352, 498, 749
BseDI CCNNGG 4 cut(s) 132, 351, 747, 928
BseGI GGATG 3 cut(s) 241, 571, 1162
BseLI CCNNNNNNNGG 2 cut(s) 328, 334
BseMII CTCAG 3 cut(s) 266, 377, 854
BseNI ACTGG 2 cut(s) 322, 475
BseSI GKGCMC 2 cut(s) 28, 391
BseXI GCAGC 2 cut(s) 310, 1048
BsgI GTGCAG 2 cut(s) 558, 1055
BshNI GGYRCC 1 cut(s) 343
BsiHKAI GWGCWC 2 cut(s) 28, 744
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 2 cut(s) 328, 334
BsmAI GTCTC 3 cut(s) 390, 797, 818
BsmFI GGGAC 1 cut(s) 448
Bso31I GGTCTC 1 cut(s) 818
Bsp1286I GDGCHC 3 cut(s) 28, 391, 744
Bsp143I GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
Bsp1720I GCTNAGC 1 cut(s) 363
Bsp19I CCATGG 2 cut(s) 132, 928
BspACI CCGC 2 cut(s) 385, 520
BspCNI CTCAG 3 cut(s) 265, 376, 853
BspHI TCATGA 1 cut(s) 115
BspLI GGNNCC 2 cut(s) 345, 985
BspPI GGATC 2 cut(s) 61, 459
BspT107I GGYRCC 1 cut(s) 343
BspTNI GGTCTC 1 cut(s) 818
BsrI ACTGG 2 cut(s) 322, 475
BssECI CCNNGG 4 cut(s) 132, 351, 747, 928
BssMI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
BssT1I CCWWGG 2 cut(s) 132, 928
Bst2UI CCWGG 4 cut(s) 329, 352, 498, 749
Bst4CI ACNGT 4 cut(s) 30, 45, 122, 916
Bst6I CTCTTC 1 cut(s) 537
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 1022
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 4 cut(s) 54, 252, 363, 840
BstDSI CCRYGG 2 cut(s) 132, 928
BstF5I GGATG 3 cut(s) 241, 571, 1162
BstHHI GCGC 1 cut(s) 870
BstKTI GATC 8 cut(s) 69, 454, 604, 609, 690, 817, 980, 1012
BstMAI GTCTC 3 cut(s) 390, 797, 818
BstMBI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
BstMWI GCNNNNNNNGC 3 cut(s) 11, 739, 1045
BstNI CCWGG 4 cut(s) 329, 352, 498, 749
BstNSI RCATGY 2 cut(s) 482, 517
BstSCI CCNGG 4 cut(s) 327, 350, 496, 747
BstSLI GKGCMC 2 cut(s) 28, 391
BstV1I GCAGC 2 cut(s) 310, 1048
BstX2I RGATCY 2 cut(s) 66, 606
BstYI RGATCY 2 cut(s) 66, 606
BtgI CCRYGG 2 cut(s) 132, 928
BtrI CACGTC 1 cut(s) 503
BtsCI GGATG 3 cut(s) 241, 571, 1162
BtsIMutI CAGTG 2 cut(s) 329, 912
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 628
CciI TCATGA 1 cut(s) 115
CfoI GCGC 1 cut(s) 870
Cfr13I GGNCC 2 cut(s) 319, 940
CviAII CATG 7 cut(s) 116, 133, 419, 479, 514, 929, 964
DdeI CTNAG 4 cut(s) 54, 252, 363, 840
DpnI GATC 8 cut(s) 68, 453, 603, 608, 689, 816, 979, 1011
DpnII GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
DraIII CACNNNGTG 1 cut(s) 916
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 1 cut(s) 742
Eco130I CCWWGG 2 cut(s) 132, 928
Eco24I GRGCYC 1 cut(s) 744
Eco31I GGTCTC 1 cut(s) 818
Eco47I GGWCC 2 cut(s) 319, 940
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 3 cut(s) 99, 449, 696
Eco72I CACGTG 1 cut(s) 1022
EcoICRI GAGCTC 1 cut(s) 742
EcoRI GAATTC 2 cut(s) 459, 971
EcoRII CCWGG 4 cut(s) 327, 350, 496, 747
EcoT14I CCWWGG 2 cut(s) 132, 928
EcoT38I GRGCYC 1 cut(s) 744
ErhI CCWWGG 2 cut(s) 132, 928
FaeI CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
FalI AAGNNNNNCTT 4 cut(s) 135, 167, 1032, 1064
FaqI GGGAC 1 cut(s) 448
FatI CATG 7 cut(s) 115, 132, 418, 478, 513, 928, 963
FauI CCCGC 1 cut(s) 378
Fnu4HI GCNGC 2 cut(s) 299, 1037
FokI GGATG 2 cut(s) 248, 578
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 2 cut(s) 299, 1037
FspBI CTAG 3 cut(s) 438, 588, 1152
GlaI GCGC 1 cut(s) 869
GluI GCNGC 2 cut(s) 299, 1037
GsuI CTGGAG 3 cut(s) 492, 770, 821
HhaI GCGC 1 cut(s) 870
Hin1II CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
Hin6I GCGC 1 cut(s) 868
HinP1I GCGC 1 cut(s) 868
HinfI GANTC 5 cut(s) 50, 196, 241, 756, 1072
HphI GGTGA 3 cut(s) 682, 736, 944
Hpy166II GTNNAC 2 cut(s) 26, 140
Hpy188I TCNGA 6 cut(s) 79, 468, 606, 843, 977, 1103
Hpy188III TCNNGA 8 cut(s) 17, 86, 116, 258, 288, 446, 674, 1147
Hpy8I GTNNAC 2 cut(s) 26, 140
HpyAV CCTTC 7 cut(s) 14, 74, 198, 370, 406, 642, 1100
HpyCH4III ACNGT 4 cut(s) 30, 45, 122, 916
HpyCH4IV ACGT 2 cut(s) 502, 1021
HpyCH4V TGCA 6 cut(s) 26, 298, 478, 539, 833, 1036
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 739, 1045
HpyF3I CTNAG 4 cut(s) 54, 252, 363, 840
HpySE526I ACGT 2 cut(s) 502, 1021
Hsp92II CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
HspAI GCGC 1 cut(s) 868
Kzo9I GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
LmnI GCTCC 1 cut(s) 19
Lsp1109I GCAGC 2 cut(s) 310, 1048
LweI GCATC 3 cut(s) 25, 118, 885
MaeI CTAG 3 cut(s) 438, 588, 1152
MaeII ACGT 2 cut(s) 502, 1021
MaeIII GTNAC 3 cut(s) 905, 953, 1147
MalI GATC 8 cut(s) 68, 453, 603, 608, 689, 816, 979, 1011
MboI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
MboII GAAGA 5 cut(s) 85, 455, 524, 800, 1134
MflI RGATCY 2 cut(s) 66, 606
MhlI GDGCHC 3 cut(s) 28, 391, 744
MluCI AATT 8 cut(s) 210, 291, 459, 764, 971, 1053, 1057, 1141
MlyI GAGTC 1 cut(s) 1066
MnlI CCTC 8 cut(s) 80, 103, 200, 442, 516, 681, 745, 997
MseI TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
MslI CAYNNNNRTG 3 cut(s) 423, 551, 962
MspR9I CCNGG 4 cut(s) 329, 352, 498, 749
MvaI CCWGG 4 cut(s) 329, 352, 498, 749
MwoI GCNNNNNNNGC 3 cut(s) 11, 739, 1045
NcoI CCATGG 2 cut(s) 132, 928
NdeII GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
NlaIII CATG 7 cut(s) 119, 136, 422, 482, 517, 932, 967
NlaIV GGNNCC 2 cut(s) 345, 985
NmeAIII GCCGAG 1 cut(s) 384
NmuCI GTSAC 3 cut(s) 905, 953, 1147
NspI RCATGY 2 cut(s) 482, 517
PagI TCATGA 1 cut(s) 115
PciI ACATGT 1 cut(s) 513
PcsI WCGNNNNNNNCGW 2 cut(s) 1102, 1111
PfeI GAWTC 4 cut(s) 50, 196, 241, 756
PflMI CCANNNNNTGG 1 cut(s) 334
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 2 cut(s) 300, 1038
PleI GAGTC 1 cut(s) 1066
PmaCI CACGTG 1 cut(s) 1022
PmlI CACGTG 1 cut(s) 1022
PpsI GAGTC 1 cut(s) 1066
Ppu21I YACGTR 1 cut(s) 1022
PscI ACATGT 1 cut(s) 513
PshBI ATTAAT 1 cut(s) 1056
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 4 cut(s) 327, 350, 496, 747
PspCI CACGTG 1 cut(s) 1022
PspGI CCWGG 4 cut(s) 327, 350, 496, 747
PspN4I GGNNCC 2 cut(s) 345, 985
PspPI GGNCC 2 cut(s) 319, 940
PstNI CAGNNNCTG 1 cut(s) 628
PsuI RGATCY 2 cut(s) 66, 606
RseI CAYNNNNRTG 3 cut(s) 423, 551, 962
SacI GAGCTC 1 cut(s) 744
SaqAI TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
SatI GCNGC 2 cut(s) 299, 1037
Sau3AI GATC 8 cut(s) 66, 451, 601, 606, 687, 814, 977, 1009
Sau96I GGNCC 2 cut(s) 319, 940
SchI GAGTC 1 cut(s) 1066
ScrFI CCNGG 4 cut(s) 329, 352, 498, 749
SduI GDGCHC 3 cut(s) 28, 391, 744
SfaNI GCATC 3 cut(s) 25, 118, 885
SinI GGWCC 2 cut(s) 319, 940
SmiMI CAYNNNNRTG 3 cut(s) 423, 551, 962
SmlI CTYRAG 1 cut(s) 683
SmoI CTYRAG 1 cut(s) 683
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 8 cut(s) 210, 291, 459, 764, 971, 1053, 1057, 1141
SsiI CCGC 2 cut(s) 385, 520
SspMI CTAG 3 cut(s) 438, 588, 1152
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 4 cut(s) 327, 350, 496, 747
StyI CCWWGG 2 cut(s) 132, 928
TaaI ACNGT 4 cut(s) 30, 45, 122, 916
TaiI ACGT 2 cut(s) 505, 1024
TaqI TCGA 1 cut(s) 780
TasI AATT 8 cut(s) 210, 291, 459, 764, 971, 1053, 1057, 1141
TfiI GAWTC 4 cut(s) 50, 196, 241, 756
Tru1I TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
Tru9I TTAA 6 cut(s) 155, 549, 729, 774, 858, 1056
TscAI CASTG 2 cut(s) 329, 919
TseFI GTSAC 3 cut(s) 905, 953, 1147
TseI GCWGC 2 cut(s) 298, 1036
Tsp45I GTSAC 3 cut(s) 905, 953, 1147
TspDTI ATGAA 7 cut(s) 74, 104, 407, 582, 591, 719, 1082
TspRI CASTG 2 cut(s) 329, 919
Van91I CCANNNNNTGG 1 cut(s) 334
VneI GTGCAC 1 cut(s) 24
VpaK11BI GGWCC 2 cut(s) 319, 940
VspI ATTAAT 1 cut(s) 1056
XapI RAATTY 3 cut(s) 291, 459, 971
XceI RCATGY 2 cut(s) 482, 517
XspI CTAG 3 cut(s) 438, 588, 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.