Belongs to the DEAD box helicase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1301 |
| Acc36I | ACCTGC | 1 cut(s) | 1432 |
| AccB1I | GGYRCC | 1 cut(s) | 7 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 157 |
| AccI | GTMKAC | 1 cut(s) | 252 |
| AccII | CGCG | 1 cut(s) | 231 |
| AciI | CCGC | 3 cut(s) | 163, 787, 922 |
| AclWI | GGATC | 3 cut(s) | 433, 484, 1011 |
| AcoI | YGGCCR | 1 cut(s) | 148 |
| AcsI | RAATTY | 2 cut(s) | 693, 1041 |
| AcyI | GRCGYC | 1 cut(s) | 8 |
| AfaI | GTAC | 1 cut(s) | 895 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 157, 270, 271, 559 |
| AflIII | ACRYGT | 3 cut(s) | 229, 859, 915 |
| AgsI | TTSAA | 4 cut(s) | 227, 602, 647, 1000 |
| AhlI | ACTAGT | 1 cut(s) | 736 |
| AjnI | CCWGG | 3 cut(s) | 898, 1094, 1265 |
| AluBI | AGCT | 9 cut(s) | 126, 286, 381, 469, 706, 974, 1027, 1144, 1615 |
| AluI | AGCT | 9 cut(s) | 126, 286, 381, 469, 706, 974, 1027, 1144, 1615 |
| Alw21I | GWGCWC | 3 cut(s) | 383, 1146, 1401 |
| Alw26I | GTCTC | 8 cut(s) | 61, 208, 919, 936, 1199, 1220, 1324, 1450 |
| AlwI | GGATC | 3 cut(s) | 433, 484, 1011 |
| Ama87I | CYCGRG | 1 cut(s) | 1202 |
| AoxI | GGCC | 5 cut(s) | 148, 160, 279, 715, 1419 |
| ApeKI | GCWGC | 5 cut(s) | 413, 703, 1027, 1302, 1615 |
| ApoI | RAATTY | 2 cut(s) | 693, 1041 |
| AspLEI | GCGC | 4 cut(s) | 10, 413, 1272, 1302 |
| AspS9I | GGNCC | 4 cut(s) | 160, 279, 837, 1420 |
| AsuHPI | GGTGA | 8 cut(s) | 64, 351, 490, 809, 1084, 1141, 1231, 1346 |
| AvaI | CYCGRG | 1 cut(s) | 1202 |
| AvaII | GGWCC | 1 cut(s) | 837 |
| BalI | TGGCCA | 1 cut(s) | 150 |
| BanI | GGYRCC | 1 cut(s) | 7 |
| BanII | GRGCYC | 3 cut(s) | 383, 793, 1146 |
| BauI | CACGAG | 2 cut(s) | 990, 1392 |
| BbsI | GAAGAC | 2 cut(s) | 17, 28 |
| Bbv12I | GWGCWC | 3 cut(s) | 383, 1146, 1401 |
| BbvI | GCAGC | 5 cut(s) | 400, 715, 1014, 1314, 1602 |
| BccI | CCATC | 2 cut(s) | 50, 476 |
| BceAI | ACGGC | 2 cut(s) | 21, 1290 |
| BciT130I | CCWGG | 3 cut(s) | 900, 1096, 1267 |
| BclI | TGATCA | 1 cut(s) | 1309 |
| BcoDI | GTCTC | 8 cut(s) | 61, 208, 919, 936, 1199, 1220, 1324, 1450 |
| BcuI | ACTAGT | 1 cut(s) | 736 |
| BfaI | CTAG | 6 cut(s) | 32, 119, 287, 737, 870, 1353 |
| BfmI | CTRYAG | 2 cut(s) | 51, 542 |
| BfoI | RGCGCY | 2 cut(s) | 11, 414 |
| BfuAI | ACCTGC | 1 cut(s) | 1432 |
| BglII | AGATCT | 1 cut(s) | 1008 |
| BisI | GCNGC | 6 cut(s) | 163, 414, 704, 1028, 1303, 1616 |
| BlsI | GCNGC | 6 cut(s) | 164, 415, 705, 1029, 1304, 1617 |
| Bme1390I | CCNGG | 3 cut(s) | 900, 1096, 1267 |
| Bme18I | GGWCC | 1 cut(s) | 837 |
| BmeT110I | CYCGRG | 1 cut(s) | 1202 |
| BmgT120I | GGNCC | 4 cut(s) | 160, 279, 837, 1420 |
| BmiI | GGNNCC | 2 cut(s) | 9, 838 |
| BmrFI | CCNGG | 3 cut(s) | 900, 1096, 1267 |
| BmsI | GCATC | 3 cut(s) | 442, 573, 1287 |
| BoxI | GACNNNNGTC | 1 cut(s) | 441 |
| BpiI | GAAGAC | 2 cut(s) | 17, 28 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 575, 607, 916, 948 |
| BpmI | CTGGAG | 1 cut(s) | 226 |
| BpuEI | CTTGAG | 1 cut(s) | 1172 |
| BsaHI | GRCGYC | 1 cut(s) | 8 |
| BsaI | GGTCTC | 3 cut(s) | 1220, 1324, 1450 |
| BsaJI | CCNNGG | 4 cut(s) | 1230, 1330, 1423, 1554 |
| BsaWI | WCCGGW | 1 cut(s) | 418 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 867, 897, 918, 948, 1516, 1546 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 157, 270, 271, 559 |
| Bse118I | RCCGGY | 1 cut(s) | 1618 |
| Bse1I | ACTGG | 3 cut(s) | 243, 670, 840 |
| BseBI | CCWGG | 3 cut(s) | 900, 1096, 1267 |
| BseDI | CCNNGG | 4 cut(s) | 1230, 1330, 1423, 1554 |
| BseGI | GGATG | 4 cut(s) | 960, 1564, 1575, 1581 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 157, 270, 271, 559 |
| BseMII | CTCAG | 2 cut(s) | 72, 571 |
| BseNI | ACTGG | 3 cut(s) | 243, 670, 840 |
| BseRI | GAGGAG | 3 cut(s) | 96, 1205, 1625 |
| BseXI | GCAGC | 5 cut(s) | 400, 715, 1014, 1314, 1602 |
| BseYI | CCCAGC | 1 cut(s) | 276 |
| BsgI | GTGCAG | 1 cut(s) | 399 |
| Bsh1236I | CGCG | 1 cut(s) | 231 |
| BshFI | GGCC | 5 cut(s) | 150, 162, 281, 717, 1421 |
| BshNI | GGYRCC | 1 cut(s) | 7 |
| BsiHKAI | GWGCWC | 3 cut(s) | 383, 1146, 1401 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1202 |
| BsiSI | CCGG | 4 cut(s) | 176, 419, 1481, 1619 |
| BslFI | GGGAC | 4 cut(s) | 552, 645, 850, 1370 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 157, 270, 271, 559 |
| BsmAI | GTCTC | 8 cut(s) | 61, 208, 919, 936, 1199, 1220, 1324, 1450 |
| BsmBI | CGTCTC | 2 cut(s) | 208, 1199 |
| BsmFI | GGGAC | 4 cut(s) | 552, 645, 850, 1370 |
| BsnI | GGCC | 5 cut(s) | 150, 162, 281, 717, 1421 |
| Bso31I | GGTCTC | 3 cut(s) | 1220, 1324, 1450 |
| BsoBI | CYCGRG | 1 cut(s) | 1202 |
| Bsp1286I | GDGCHC | 4 cut(s) | 383, 793, 1146, 1401 |
| Bsp143I | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| Bsp19I | CCATGG | 1 cut(s) | 1330 |
| BspACI | CCGC | 3 cut(s) | 163, 787, 922 |
| BspANI | GGCC | 5 cut(s) | 150, 162, 281, 717, 1421 |
| BspCNI | CTCAG | 2 cut(s) | 71, 570 |
| BspFNI | CGCG | 1 cut(s) | 231 |
| BspHI | TCATGA | 3 cut(s) | 517, 1104, 1365 |
| BspLI | GGNNCC | 2 cut(s) | 9, 838 |
| BspMAI | CTGCAG | 2 cut(s) | 55, 546 |
| BspMI | ACCTGC | 1 cut(s) | 1432 |
| BspPI | GGATC | 3 cut(s) | 433, 484, 1011 |
| BspT107I | GGYRCC | 1 cut(s) | 7 |
| BspTNI | GGTCTC | 3 cut(s) | 1220, 1324, 1450 |
| BsrFI | RCCGGY | 1 cut(s) | 1618 |
| BsrI | ACTGG | 3 cut(s) | 243, 670, 840 |
| BssAI | RCCGGY | 1 cut(s) | 1618 |
| BssECI | CCNNGG | 4 cut(s) | 1230, 1330, 1423, 1554 |
| BssMI | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| BssNI | GRCGYC | 1 cut(s) | 8 |
| BssSI | CACGAG | 2 cut(s) | 990, 1392 |
| BssT1I | CCWWGG | 3 cut(s) | 1230, 1330, 1554 |
| Bst2BI | CACGAG | 2 cut(s) | 990, 1392 |
| Bst2UI | CCWGG | 3 cut(s) | 900, 1096, 1267 |
| Bst4CI | ACNGT | 3 cut(s) | 524, 930, 1318 |
| BstACI | GRCGYC | 1 cut(s) | 8 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 128 |
| BstC8I | GCNNGC | 5 cut(s) | 51, 124, 409, 789, 1620 |
| BstDEI | CTNAG | 2 cut(s) | 58, 557 |
| BstDSI | CCRYGG | 2 cut(s) | 1330, 1423 |
| BstF5I | GGATG | 4 cut(s) | 960, 1564, 1575, 1581 |
| BstFNI | CGCG | 1 cut(s) | 231 |
| BstH2I | RGCGCY | 2 cut(s) | 11, 414 |
| BstHHI | GCGC | 4 cut(s) | 10, 413, 1272, 1302 |
| BstKTI | GATC | 9 cut(s) | 428, 479, 856, 1006, 1011, 1219, 1312, 1414, 1636 |
| BstMAI | GTCTC | 8 cut(s) | 61, 208, 919, 936, 1199, 1220, 1324, 1450 |
| BstMBI | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 128, 168, 413, 1628 |
| BstNI | CCWGG | 3 cut(s) | 900, 1096, 1267 |
| BstNSI | RCATGY | 2 cut(s) | 169, 919 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 441 |
| BstSCI | CCNGG | 3 cut(s) | 898, 1094, 1265 |
| BstSFI | CTRYAG | 2 cut(s) | 51, 542 |
| BstUI | CGCG | 1 cut(s) | 231 |
| BstV1I | GCAGC | 5 cut(s) | 400, 715, 1014, 1314, 1602 |
| BstV2I | GAAGAC | 2 cut(s) | 17, 28 |
| BstX2I | RGATCY | 3 cut(s) | 425, 476, 1008 |
| BstYI | RGATCY | 3 cut(s) | 425, 476, 1008 |
| BsuRI | GGCC | 5 cut(s) | 150, 162, 281, 717, 1421 |
| BtgI | CCRYGG | 2 cut(s) | 1330, 1423 |
| BtsCI | GGATG | 4 cut(s) | 960, 1564, 1575, 1581 |
| BtsIMutI | CAGTG | 1 cut(s) | 847 |
| BveI | ACCTGC | 1 cut(s) | 1432 |
| Cac8I | GCNNGC | 5 cut(s) | 51, 124, 409, 789, 1620 |
| CciI | TCATGA | 3 cut(s) | 517, 1104, 1365 |
| CfoI | GCGC | 4 cut(s) | 10, 413, 1272, 1302 |
| Cfr10I | RCCGGY | 1 cut(s) | 1618 |
| Cfr13I | GGNCC | 4 cut(s) | 160, 279, 837, 1420 |
| CseI | GACGC | 4 cut(s) | 190, 220, 446, 1216 |
| CsiI | ACCWGGT | 1 cut(s) | 898 |
| Csp6I | GTAC | 1 cut(s) | 894 |
| CviQI | GTAC | 1 cut(s) | 894 |
| DdeI | CTNAG | 2 cut(s) | 58, 557 |
| DinI | GGCGCC | 1 cut(s) | 9 |
| DpnI | GATC | 9 cut(s) | 427, 478, 855, 1005, 1010, 1218, 1311, 1413, 1635 |
| DpnII | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| EaeI | YGGCCR | 1 cut(s) | 148 |
| Ecl136II | GAGCTC | 2 cut(s) | 381, 1144 |
| Eco130I | CCWWGG | 3 cut(s) | 1230, 1330, 1554 |
| Eco24I | GRGCYC | 3 cut(s) | 383, 793, 1146 |
| Eco31I | GGTCTC | 3 cut(s) | 1220, 1324, 1450 |
| Eco32I | GATATC | 1 cut(s) | 1090 |
| Eco47I | GGWCC | 1 cut(s) | 837 |
| Eco53kI | GAGCTC | 2 cut(s) | 381, 1144 |
| Eco88I | CYCGRG | 1 cut(s) | 1202 |
| EcoICRI | GAGCTC | 2 cut(s) | 381, 1144 |
| EcoRII | CCWGG | 3 cut(s) | 898, 1094, 1265 |
| EcoRV | GATATC | 1 cut(s) | 1090 |
| EcoT14I | CCWWGG | 3 cut(s) | 1230, 1330, 1554 |
| EcoT22I | ATGCAT | 1 cut(s) | 574 |
| EcoT38I | GRGCYC | 3 cut(s) | 383, 793, 1146 |
| EgeI | GGCGCC | 1 cut(s) | 9 |
| EheI | GGCGCC | 1 cut(s) | 9 |
| ErhI | CCWWGG | 3 cut(s) | 1230, 1330, 1554 |
| Esp3I | CGTCTC | 2 cut(s) | 208, 1199 |
| FaqI | GGGAC | 4 cut(s) | 552, 645, 850, 1370 |
| FauI | CCCGC | 1 cut(s) | 780 |
| FauNDI | CATATG | 1 cut(s) | 574 |
| FbaI | TGATCA | 1 cut(s) | 1309 |
| FblI | GTMKAC | 1 cut(s) | 252 |
| Fnu4HI | GCNGC | 6 cut(s) | 163, 414, 704, 1028, 1303, 1616 |
| FokI | GGATG | 4 cut(s) | 967, 1568, 1571, 1582 |
| FriOI | GRGCYC | 3 cut(s) | 383, 793, 1146 |
| Fsp4HI | GCNGC | 6 cut(s) | 163, 414, 704, 1028, 1303, 1616 |
| FspBI | CTAG | 6 cut(s) | 32, 119, 287, 737, 870, 1353 |
| FspI | TGCGCA | 1 cut(s) | 1301 |
| GlaI | GCGC | 4 cut(s) | 9, 412, 1271, 1301 |
| GluI | GCNGC | 6 cut(s) | 163, 414, 704, 1028, 1303, 1616 |
| GsaI | CCCAGC | 1 cut(s) | 280 |
| GsuI | CTGGAG | 1 cut(s) | 226 |
| HaeII | RGCGCY | 2 cut(s) | 11, 414 |
| HaeIII | GGCC | 5 cut(s) | 150, 162, 281, 717, 1421 |
| HapII | CCGG | 4 cut(s) | 176, 419, 1481, 1619 |
| HgaI | GACGC | 4 cut(s) | 190, 220, 446, 1216 |
| HhaI | GCGC | 4 cut(s) | 10, 413, 1272, 1302 |
| Hin1I | GRCGYC | 1 cut(s) | 8 |
| Hin6I | GCGC | 4 cut(s) | 8, 411, 1270, 1300 |
| HinP1I | GCGC | 4 cut(s) | 8, 411, 1270, 1300 |
| HincII | GTYRAC | 1 cut(s) | 1183 |
| HindII | GTYRAC | 1 cut(s) | 1183 |
| HinfI | GANTC | 4 cut(s) | 143, 364, 643, 876 |
| HpaII | CCGG | 4 cut(s) | 176, 419, 1481, 1619 |
| HphI | GGTGA | 8 cut(s) | 64, 351, 490, 809, 1084, 1141, 1231, 1346 |
| Hpy166II | GTNNAC | 5 cut(s) | 253, 758, 896, 1183, 1261 |
| Hpy188I | TCNGA | 5 cut(s) | 61, 139, 378, 481, 1008 |
| Hpy8I | GTNNAC | 5 cut(s) | 253, 758, 896, 1183, 1261 |
| Hpy99I | CGWCG | 2 cut(s) | 236, 260 |
| HpyCH4III | ACNGT | 3 cut(s) | 524, 930, 1318 |
| HpyCH4IV | ACGT | 5 cut(s) | 22, 258, 267, 859, 1135 |
| HpyCH4V | TGCA | 8 cut(s) | 53, 131, 416, 544, 572, 596, 627, 746 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 128, 168, 413, 1628 |
| HpyF3I | CTNAG | 2 cut(s) | 58, 557 |
| HpySE526I | ACGT | 5 cut(s) | 22, 258, 267, 859, 1135 |
| Hsp92I | GRCGYC | 1 cut(s) | 8 |
| HspAI | GCGC | 4 cut(s) | 8, 411, 1270, 1300 |
| KasI | GGCGCC | 1 cut(s) | 7 |
| KroI | GCCGGC | 1 cut(s) | 1618 |
| KroNI | GCCGGC | 1 cut(s) | 1620 |
| Ksp22I | TGATCA | 1 cut(s) | 1309 |
| Kzo9I | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| LmnI | GCTCC | 2 cut(s) | 1342, 1612 |
| Lsp1109I | GCAGC | 5 cut(s) | 400, 715, 1014, 1314, 1602 |
| LweI | GCATC | 3 cut(s) | 442, 573, 1287 |
| MabI | ACCWGGT | 1 cut(s) | 898 |
| MaeI | CTAG | 6 cut(s) | 32, 119, 287, 737, 870, 1353 |
| MaeII | ACGT | 5 cut(s) | 22, 258, 267, 859, 1135 |
| MaeIII | GTNAC | 4 cut(s) | 259, 797, 1334, 1549 |
| MalI | GATC | 9 cut(s) | 427, 478, 855, 1005, 1010, 1218, 1311, 1413, 1635 |
| MboI | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| MboII | GAAGA | 8 cut(s) | 17, 28, 211, 469, 487, 863, 1380, 1628 |
| MflI | RGATCY | 3 cut(s) | 425, 476, 1008 |
| MhlI | GDGCHC | 4 cut(s) | 383, 793, 1146, 1401 |
| MlsI | TGGCCA | 1 cut(s) | 150 |
| MluCI | AATT | 7 cut(s) | 132, 431, 597, 612, 693, 1041, 1605 |
| MluI | ACGCGT | 1 cut(s) | 229 |
| MluNI | TGGCCA | 1 cut(s) | 150 |
| Mly113I | GGCGCC | 1 cut(s) | 8 |
| MlyI | GAGTC | 3 cut(s) | 137, 358, 885 |
| MmeI | TCCRAC | 1 cut(s) | 1496 |
| Mox20I | TGGCCA | 1 cut(s) | 150 |
| Mph1103I | ATGCAT | 1 cut(s) | 574 |
| MroNI | GCCGGC | 1 cut(s) | 1618 |
| MscI | TGGCCA | 1 cut(s) | 150 |
| MseI | TTAA | 3 cut(s) | 350, 1458, 1642 |
| MslI | CAYNNNNRTG | 4 cut(s) | 825, 1176, 1256, 1364 |
| Msp20I | TGGCCA | 1 cut(s) | 150 |
| MspA1I | CMGCKG | 1 cut(s) | 1027 |
| MspI | CCGG | 4 cut(s) | 176, 419, 1481, 1619 |
| MspR9I | CCNGG | 3 cut(s) | 900, 1096, 1267 |
| MvaI | CCWGG | 3 cut(s) | 900, 1096, 1267 |
| MvnI | CGCG | 1 cut(s) | 231 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 128, 168, 413, 1628 |
| NaeI | GCCGGC | 1 cut(s) | 1620 |
| NarI | GGCGCC | 1 cut(s) | 8 |
| NcoI | CCATGG | 1 cut(s) | 1330 |
| NdeI | CATATG | 1 cut(s) | 574 |
| NdeII | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| NgoMIV | GCCGGC | 1 cut(s) | 1618 |
| NlaIV | GGNNCC | 2 cut(s) | 9, 838 |
| NmeAIII | GCCGAG | 1 cut(s) | 307 |
| NmuCI | GTSAC | 3 cut(s) | 797, 1334, 1549 |
| NsbI | TGCGCA | 1 cut(s) | 1301 |
| NsiI | ATGCAT | 1 cut(s) | 574 |
| NspI | RCATGY | 2 cut(s) | 169, 919 |
| PaeR7I | CTCGAG | 1 cut(s) | 1202 |
| PagI | TCATGA | 3 cut(s) | 517, 1104, 1365 |
| PciI | ACATGT | 1 cut(s) | 915 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 264, 1504 |
| PdiI | GCCGGC | 1 cut(s) | 1620 |
| PfeI | GAWTC | 1 cut(s) | 643 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 157 |
| PkrI | GCNGC | 6 cut(s) | 164, 415, 705, 1029, 1304, 1617 |
| PleI | GAGTC | 3 cut(s) | 137, 358, 884 |
| PluTI | GGCGCC | 1 cut(s) | 11 |
| PpsI | GAGTC | 3 cut(s) | 137, 358, 884 |
| PscI | ACATGT | 1 cut(s) | 915 |
| PshAI | GACNNNNGTC | 1 cut(s) | 441 |
| Psp124BI | GAGCTC | 2 cut(s) | 383, 1146 |
| Psp6I | CCWGG | 3 cut(s) | 898, 1094, 1265 |
| PspFI | CCCAGC | 1 cut(s) | 276 |
| PspGI | CCWGG | 3 cut(s) | 898, 1094, 1265 |
| PspN4I | GGNNCC | 2 cut(s) | 9, 838 |
| PspPI | GGNCC | 4 cut(s) | 160, 279, 837, 1420 |
| PstI | CTGCAG | 2 cut(s) | 55, 546 |
| PsuI | RGATCY | 3 cut(s) | 425, 476, 1008 |
| PvuII | CAGCTG | 1 cut(s) | 1027 |
| RsaI | GTAC | 1 cut(s) | 895 |
| RsaNI | GTAC | 1 cut(s) | 894 |
| RseI | CAYNNNNRTG | 4 cut(s) | 825, 1176, 1256, 1364 |
| SacI | GAGCTC | 2 cut(s) | 383, 1146 |
| SaqAI | TTAA | 3 cut(s) | 350, 1458, 1642 |
| SatI | GCNGC | 6 cut(s) | 163, 414, 704, 1028, 1303, 1616 |
| Sau3AI | GATC | 9 cut(s) | 425, 476, 853, 1003, 1008, 1216, 1309, 1411, 1633 |
| Sau96I | GGNCC | 4 cut(s) | 160, 279, 837, 1420 |
| SchI | GAGTC | 3 cut(s) | 137, 358, 885 |
| ScrFI | CCNGG | 3 cut(s) | 900, 1096, 1267 |
| SduI | GDGCHC | 4 cut(s) | 383, 793, 1146, 1401 |
| SexAI | ACCWGGT | 1 cut(s) | 898 |
| SfaNI | GCATC | 3 cut(s) | 442, 573, 1287 |
| SfcI | CTRYAG | 2 cut(s) | 51, 542 |
| SfoI | GGCGCC | 1 cut(s) | 9 |
| Sfr274I | CTCGAG | 1 cut(s) | 1202 |
| SinI | GGWCC | 1 cut(s) | 837 |
| SlaI | CTCGAG | 1 cut(s) | 1202 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 825, 1176, 1256, 1364 |
| SmlI | CTYRAG | 2 cut(s) | 1151, 1202 |
| SmoI | CTYRAG | 2 cut(s) | 1151, 1202 |
| SpeI | ACTAGT | 1 cut(s) | 736 |
| Sse9I | AATT | 7 cut(s) | 132, 431, 597, 612, 693, 1041, 1605 |
| SsiI | CCGC | 3 cut(s) | 163, 787, 922 |
| SspDI | GGCGCC | 1 cut(s) | 7 |
| SspMI | CTAG | 6 cut(s) | 32, 119, 287, 737, 870, 1353 |
| SstI | GAGCTC | 2 cut(s) | 383, 1146 |
| StyD4I | CCNGG | 3 cut(s) | 898, 1094, 1265 |
| StyI | CCWWGG | 3 cut(s) | 1230, 1330, 1554 |
| TaaI | ACNGT | 3 cut(s) | 524, 930, 1318 |
| TaiI | ACGT | 5 cut(s) | 25, 261, 270, 862, 1138 |
| TaqI | TCGA | 4 cut(s) | 723, 904, 1203, 1578 |
| TasI | AATT | 7 cut(s) | 132, 431, 597, 612, 693, 1041, 1605 |
| TauI | GCSGC | 1 cut(s) | 165 |
| TfiI | GAWTC | 1 cut(s) | 643 |
| Tru1I | TTAA | 3 cut(s) | 350, 1458, 1642 |
| Tru9I | TTAA | 3 cut(s) | 350, 1458, 1642 |
| TscAI | CASTG | 1 cut(s) | 847 |
| TseFI | GTSAC | 3 cut(s) | 797, 1334, 1549 |
| TseI | GCWGC | 5 cut(s) | 413, 703, 1027, 1302, 1615 |
| Tsp45I | GTSAC | 3 cut(s) | 797, 1334, 1549 |
| TspDTI | ATGAA | 9 cut(s) | 476, 506, 809, 984, 993, 1093, 1121, 1484, 1578 |
| TspGWI | ACGGA | 1 cut(s) | 926 |
| TspRI | CASTG | 1 cut(s) | 847 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 157 |
| VpaK11BI | GGWCC | 1 cut(s) | 837 |
| XapI | RAATTY | 2 cut(s) | 693, 1041 |
| XceI | RCATGY | 2 cut(s) | 169, 919 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 887, 1045 |
| XhoI | CTCGAG | 1 cut(s) | 1202 |
| XmiI | GTMKAC | 1 cut(s) | 252 |
| XspI | CTAG | 6 cut(s) | 32, 119, 287, 737, 870, 1353 |
| Zsp2I | ATGCAT | 1 cut(s) | 574 |