MD17G1172700.v1.1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000799
Physical Location & Seq
Forward (+)
17810856 .. 17813663
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1172700.v1.1.491

Sequence Viewer

Length: 1185 bp
ATGAGTGAGCTGCTCTCAACTGATGGGCAAGATTTCTTCACATCGTATGATGAAGTTTTTGAAAGTTTTGATGCCATGGGATTGCAGGAAAATCTTCTGAGAGGCATTTATGCGTATGGGTTTGAGAAGCCTTCTGCCATTCAGCAAAGGGGAATTGTTCCATTCTGCAAAGGCCTTGATGTGATTCAACAGGCTCAGTCCGGAACCGGGAAAACAGCAACCTTTTGCTCGGGGATTTTGCAGCAACTTGATTATGGTGTTGTCCAGTGCCAGGCTTTGGTTTTGGCACCTACCAGGGAGTTGGCACAGCAGATTGAGAAGGTTATGCGAGCACTTGGTGACTACCTTGGTGTAAAGGTTCATGCTTGTGTTGGTGGGACAAGTGTTCGTGAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTTGTTGTTGGAACCCCTGGTCGTGTGTTTGACATGTTGAGAAGGCAGTCACTTCGCCCAGATTACATCAAGATGTTTGTATTGGATGAGGCTGATGAAATGCTTTCTCGTGGTTTTAAGGATCAGATCTACGACATTTTCCAGCTCCTGCCATCAAAGGTTCAGGTTGGGGTGTTTTCTGCTACCATGCCACCTGAAGCCCTGGAAATCACAAGGAAGTTTATGAACAAACCCGTGAGGATCTTGGTAAAGCGTGATGAGCTCACCCTTGAGGGTATCAAGCAGTTCTATGTCAATGTCGATAAAGAAGAATGGAAGCTCGAGACCCTTTGTGATCTGTATGAGACCCTGGCCATCACCCAGAGTGTCATTTTCGTTAACACCAGGCGCAAGGTGGACTGGCTTACTGACAAGATGAGAAGCCGCGACCACACTGTCTCCGCCACCCACGGTGACATGGACCAGAACACTCGTGACATCATCATGCGTGAATTCCGATCTGGCTCATCCCGCGTTCTCATCACCACTGATCTCTTGGCTCGTGGTATCGATGTGCAGCAGGTATCTCTCGTAATAAACTATGATTTGCCAACTCAACCCGAGAACTACCTTCATCGTATTGGACGAAGTGGAAGGTTTGGGAGAAAGGGTGTCGCTATTAACTTTGTGACAAGGGACGACGAGAGAATGCTCTATGACATCCAGAGGTTTTACAATGTGGTGATCGAGGAGCTCCCATCGAATGTTGCCGATCTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

395

Amino Acids

44.85

Weight (kDa)

5.58

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 46 - 208 6.6e-43 DEAD/DEAH box helicase
ResIII PF04851 64 - 204 6.7e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 247 - 355 7.8e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 973
AccB1I GGYRCC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 277
AccII CGCG 2 cut(s) 849, 936
AccIII TCCGGA 1 cut(s) 200
AciI CCGC 3 cut(s) 847, 864, 934
AclWI GGATC 3 cut(s) 402, 552, 671
AcoI YGGCCR 1 cut(s) 774
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 1 cut(s) 639
AdeI CACNNNGTG 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 207, 271, 277
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 3 cut(s) 62, 188, 410
AjnI CCWGG 6 cut(s) 270, 293, 439, 624, 771, 806
AluBI AGCT 6 cut(s) 10, 413, 568, 685, 742, 1156
AluI AGCT 6 cut(s) 10, 413, 568, 685, 742, 1156
Alw21I GWGCWC 3 cut(s) 334, 687, 1158
Alw26I GTCTC 3 cut(s) 740, 761, 865
AlwI GGATC 3 cut(s) 402, 552, 671
AlwNI CAGNNNCTG 1 cut(s) 571
Ama87I CYCGRG 3 cut(s) 229, 743, 1022
Aor13HI TCCGGA 1 cut(s) 200
AoxI GGCC 2 cut(s) 172, 774
ApeKI GCWGC 3 cut(s) 10, 241, 979
ApoI RAATTY 1 cut(s) 914
Asp700I GAANNNNTTC 1 cut(s) 93
AspLEI GCGC 2 cut(s) 402, 813
AspS9I GGNCC 1 cut(s) 883
AsuC2I CCSGG 1 cut(s) 208
AsuHPI GGTGA 6 cut(s) 350, 679, 772, 887, 937, 1156
AvaI CYCGRG 3 cut(s) 229, 743, 1022
AvaII GGWCC 1 cut(s) 883
BalI TGGCCA 1 cut(s) 776
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 2 cut(s) 687, 1158
BauI CACGAG 3 cut(s) 531, 894, 963
Bbv12I GWGCWC 3 cut(s) 334, 687, 1158
BbvI GCAGC 2 cut(s) 253, 991
BccI CCATC 4 cut(s) 17, 583, 785, 1168
BcgI CGANNNNNNTGC 2 cut(s) 458, 492
BciT130I CCWGG 6 cut(s) 272, 295, 441, 626, 773, 808
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 3 cut(s) 740, 761, 865
BfuAI ACCTGC 1 cut(s) 973
BglII AGATCT 1 cut(s) 549
BisI GCNGC 4 cut(s) 11, 242, 847, 980
BlsI GCNGC 4 cut(s) 12, 243, 848, 981
Bme1390I CCNGG 7 cut(s) 208, 272, 295, 441, 626, 773, 808
Bme18I GGWCC 1 cut(s) 883
BmeT110I CYCGRG 3 cut(s) 229, 743, 1022
BmgT120I GGNCC 1 cut(s) 883
BmiI GGNNCC 3 cut(s) 205, 288, 436
BmrFI CCNGG 7 cut(s) 208, 272, 295, 441, 626, 773, 808
BmsI GCATC 1 cut(s) 61
BplI GAGNNNNNCTC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 713
BpuMI CCSGG 1 cut(s) 208
Bsa29I ATCGAT 1 cut(s) 972
BsaI GGTCTC 2 cut(s) 740, 761
BsaJI CCNNGG 7 cut(s) 75, 294, 346, 439, 624, 771, 871
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 4 cut(s) 845, 875, 1057, 1087
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 271, 277
Bse1I ACTGG 2 cut(s) 265, 827
BseAI TCCGGA 1 cut(s) 200
BseBI CCWGG 6 cut(s) 272, 295, 441, 626, 773, 808
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 7 cut(s) 75, 294, 346, 439, 624, 771, 871
BseGI GGATG 3 cut(s) 514, 929, 1122
BseLI CCNNNNNNNGG 3 cut(s) 207, 271, 277
BseMII CTCAG 2 cut(s) 89, 209
BseNI ACTGG 2 cut(s) 265, 827
BseRI GAGGAG 1 cut(s) 1166
BseXI GCAGC 2 cut(s) 253, 991
BsgI GTGCAG 1 cut(s) 998
Bsh1236I CGCG 2 cut(s) 849, 936
BshFI GGCC 2 cut(s) 174, 776
BshNI GGYRCC 1 cut(s) 286
BshVI ATCGAT 1 cut(s) 972
BsiHKAI GWGCWC 3 cut(s) 334, 687, 1158
BsiHKCI CYCGRG 3 cut(s) 229, 743, 1022
BsiSI CCGG 2 cut(s) 201, 207
BslFI GGGAC 2 cut(s) 391, 1112
BslI CCNNNNNNNGG 3 cut(s) 207, 271, 277
BsmAI GTCTC 3 cut(s) 740, 761, 865
BsmFI GGGAC 2 cut(s) 391, 1112
BsmI GAATGC 2 cut(s) 402, 1116
BsnI GGCC 2 cut(s) 174, 776
Bso31I GGTCTC 2 cut(s) 740, 761
BsoBI CYCGRG 3 cut(s) 229, 743, 1022
Bsp1286I GDGCHC 3 cut(s) 334, 687, 1158
Bsp13I TCCGGA 1 cut(s) 200
Bsp143I GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 3 cut(s) 847, 864, 934
BspANI GGCC 2 cut(s) 174, 776
BspCNI CTCAG 2 cut(s) 90, 208
BspDI ATCGAT 1 cut(s) 972
BspEI TCCGGA 1 cut(s) 200
BspFNI CGCG 2 cut(s) 849, 936
BspLI GGNNCC 3 cut(s) 205, 288, 436
BspMI ACCTGC 1 cut(s) 973
BspPI GGATC 3 cut(s) 402, 552, 671
BspT107I GGYRCC 1 cut(s) 286
BspTNI GGTCTC 2 cut(s) 740, 761
BsrI ACTGG 2 cut(s) 265, 827
BssECI CCNNGG 7 cut(s) 75, 294, 346, 439, 624, 771, 871
BssMI GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
BssSI CACGAG 3 cut(s) 531, 894, 963
BssT1I CCWWGG 2 cut(s) 75, 346
Bst2BI CACGAG 3 cut(s) 531, 894, 963
Bst2UI CCWGG 6 cut(s) 272, 295, 441, 626, 773, 808
Bst4CI ACNGT 2 cut(s) 859, 875
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 2 cut(s) 98, 195
BstDSI CCRYGG 2 cut(s) 75, 871
BstF5I GGATG 3 cut(s) 514, 929, 1122
BstFNI CGCG 2 cut(s) 849, 936
BstHHI GCGC 2 cut(s) 402, 813
BstKTI GATC 9 cut(s) 397, 547, 552, 666, 760, 923, 955, 1149, 1177
BstMAI GTCTC 3 cut(s) 740, 761, 865
BstMBI GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
BstMWI GCNNNNNNNGC 2 cut(s) 682, 933
BstNI CCWGG 6 cut(s) 272, 295, 441, 626, 773, 808
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 7 cut(s) 206, 270, 293, 439, 624, 771, 806
BstUI CGCG 2 cut(s) 849, 936
BstV1I GCAGC 2 cut(s) 253, 991
BstX2I RGATCY 2 cut(s) 549, 663
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 2 cut(s) 549, 663
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 2 cut(s) 174, 776
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 2 cut(s) 75, 871
BtsCI GGATG 3 cut(s) 514, 929, 1122
BtsIMutI CAGTG 3 cut(s) 272, 855, 948
BveI ACCTGC 1 cut(s) 973
Cac8I GCNNGC 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 571
CfoI GCGC 2 cut(s) 402, 813
Cfr13I GGNCC 1 cut(s) 883
ClaI ATCGAT 1 cut(s) 972
CviAII CATG 7 cut(s) 76, 362, 422, 457, 610, 880, 907
DdeI CTNAG 2 cut(s) 98, 195
DpnI GATC 9 cut(s) 396, 546, 551, 665, 759, 922, 954, 1148, 1176
DpnII GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
DraIII CACNNNGTG 1 cut(s) 338
DrdI GACNNNNNNGTC 1 cut(s) 857
DseDI GACNNNNNNGTC 1 cut(s) 857
EaeI YGGCCR 1 cut(s) 774
EciI GGCGGA 1 cut(s) 853
Ecl136II GAGCTC 2 cut(s) 685, 1156
Eco130I CCWWGG 2 cut(s) 75, 346
Eco147I AGGCCT 1 cut(s) 174
Eco24I GRGCYC 2 cut(s) 687, 1158
Eco31I GGTCTC 2 cut(s) 740, 761
Eco47I GGWCC 1 cut(s) 883
Eco53kI GAGCTC 2 cut(s) 685, 1156
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 3 cut(s) 229, 743, 1022
EcoICRI GAGCTC 2 cut(s) 685, 1156
EcoRI GAATTC 1 cut(s) 914
EcoRII CCWGG 6 cut(s) 270, 293, 439, 624, 771, 806
EcoT14I CCWWGG 2 cut(s) 75, 346
EcoT38I GRGCYC 2 cut(s) 687, 1158
ErhI CCWWGG 2 cut(s) 75, 346
FaeI CATG 7 cut(s) 79, 365, 425, 460, 613, 883, 910
FaqI GGGAC 2 cut(s) 391, 1112
FatI CATG 7 cut(s) 75, 361, 421, 456, 609, 879, 906
FauI CCCGC 1 cut(s) 941
Fnu4HI GCNGC 4 cut(s) 11, 242, 847, 980
FokI GGATG 3 cut(s) 521, 916, 1109
FriOI GRGCYC 2 cut(s) 687, 1158
Fsp4HI GCNGC 4 cut(s) 11, 242, 847, 980
GlaI GCGC 2 cut(s) 401, 812
GluI GCNGC 4 cut(s) 11, 242, 847, 980
HaeIII GGCC 2 cut(s) 174, 776
HapII CCGG 2 cut(s) 201, 207
HhaI GCGC 2 cut(s) 402, 813
Hin1II CATG 7 cut(s) 79, 365, 425, 460, 613, 883, 910
Hin6I GCGC 2 cut(s) 400, 811
HinP1I GCGC 2 cut(s) 400, 811
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 1 cut(s) 184
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 2 cut(s) 201, 207
HphI GGTGA 6 cut(s) 350, 679, 772, 887, 937, 1156
Hpy166II GTNNAC 2 cut(s) 802, 820
Hpy188I TCNGA 4 cut(s) 99, 549, 920, 1184
Hpy188III TCNNGA 6 cut(s) 201, 389, 493, 745, 896, 1126
Hpy8I GTNNAC 2 cut(s) 802, 820
Hpy99I CGWCG 1 cut(s) 1106
HpyAV CCTTC 5 cut(s) 141, 313, 459, 1043, 1050
HpyCH4III ACNGT 2 cut(s) 859, 875
HpyCH4V TGCA 4 cut(s) 85, 168, 241, 979
HpyF10VI GCNNNNNNNGC 2 cut(s) 682, 933
HpyF3I CTNAG 2 cut(s) 98, 195
Hsp92II CATG 7 cut(s) 79, 365, 425, 460, 613, 883, 910
HspAI GCGC 2 cut(s) 400, 811
Kpn2I TCCGGA 1 cut(s) 200
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
LmnI GCTCC 3 cut(s) 573, 1153, 1161
Lsp1109I GCAGC 2 cut(s) 253, 991
LweI GCATC 1 cut(s) 61
MaeIII GTNAC 5 cut(s) 338, 471, 875, 896, 1090
MalI GATC 9 cut(s) 396, 546, 551, 665, 759, 922, 954, 1148, 1176
MboI GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
MboII GAAGA 4 cut(s) 28, 86, 398, 743
MflI RGATCY 2 cut(s) 549, 663
MhlI GDGCHC 3 cut(s) 334, 687, 1158
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 2 cut(s) 153, 914
MluNI TGGCCA 1 cut(s) 776
MmeI TCCRAC 1 cut(s) 412
MnlI CCTC 7 cut(s) 95, 385, 505, 654, 688, 1122, 1144
Mox20I TGGCCA 1 cut(s) 776
MroI TCCGGA 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 93
MscI TGGCCA 1 cut(s) 776
MseI TTAA 3 cut(s) 540, 801, 1083
MslI CAYNNNNRTG 3 cut(s) 366, 494, 905
Msp20I TGGCCA 1 cut(s) 776
MspI CCGG 2 cut(s) 201, 207
MspR9I CCNGG 7 cut(s) 208, 272, 295, 441, 626, 773, 808
Mva1269I GAATGC 2 cut(s) 402, 1116
MvaI CCWGG 6 cut(s) 272, 295, 441, 626, 773, 808
MvnI CGCG 2 cut(s) 849, 936
MwoI GCNNNNNNNGC 2 cut(s) 682, 933
NciI CCSGG 1 cut(s) 208
NcoI CCATGG 1 cut(s) 75
NdeII GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
NlaIII CATG 7 cut(s) 79, 365, 425, 460, 613, 883, 910
NlaIV GGNNCC 3 cut(s) 205, 288, 436
NmuCI GTSAC 5 cut(s) 338, 471, 875, 896, 1090
NspI RCATGY 1 cut(s) 460
PaeR7I CTCGAG 1 cut(s) 743
PceI AGGCCT 1 cut(s) 174
PciI ACATGT 1 cut(s) 456
PcsI WCGNNNNNNNCGW 1 cut(s) 1045
PctI GAATGC 2 cut(s) 402, 1116
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 184
PflMI CCANNNNNTGG 1 cut(s) 277
PkrI GCNGC 4 cut(s) 12, 243, 848, 981
PscI ACATGT 1 cut(s) 456
Psp124BI GAGCTC 2 cut(s) 687, 1158
Psp6I CCWGG 6 cut(s) 270, 293, 439, 624, 771, 806
PspGI CCWGG 6 cut(s) 270, 293, 439, 624, 771, 806
PspN4I GGNNCC 3 cut(s) 205, 288, 436
PspPI GGNCC 1 cut(s) 883
PstNI CAGNNNCTG 1 cut(s) 571
PsuI RGATCY 2 cut(s) 549, 663
RseI CAYNNNNRTG 3 cut(s) 366, 494, 905
SacI GAGCTC 2 cut(s) 687, 1158
SaqAI TTAA 3 cut(s) 540, 801, 1083
SatI GCNGC 4 cut(s) 11, 242, 847, 980
Sau3AI GATC 9 cut(s) 394, 544, 549, 663, 757, 920, 952, 1146, 1174
Sau96I GGNCC 1 cut(s) 883
ScrFI CCNGG 7 cut(s) 208, 272, 295, 441, 626, 773, 808
SduI GDGCHC 3 cut(s) 334, 687, 1158
SfaNI GCATC 1 cut(s) 61
Sfr274I CTCGAG 1 cut(s) 743
SinI GGWCC 1 cut(s) 883
SlaI CTCGAG 1 cut(s) 743
SmiMI CAYNNNNRTG 3 cut(s) 366, 494, 905
SmlI CTYRAG 2 cut(s) 692, 743
SmoI CTYRAG 2 cut(s) 692, 743
Sse9I AATT 2 cut(s) 153, 914
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 3 cut(s) 847, 864, 934
SstI GAGCTC 2 cut(s) 687, 1158
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 7 cut(s) 206, 270, 293, 439, 624, 771, 806
StyI CCWWGG 2 cut(s) 75, 346
TaaI ACNGT 2 cut(s) 859, 875
TaqI TCGA 5 cut(s) 723, 744, 972, 1149, 1163
TasI AATT 2 cut(s) 153, 914
TauI GCSGC 1 cut(s) 849
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 3 cut(s) 540, 801, 1083
Tru9I TTAA 3 cut(s) 540, 801, 1083
TscAI CASTG 3 cut(s) 272, 862, 955
TseFI GTSAC 5 cut(s) 338, 471, 875, 896, 1090
TseI GCWGC 3 cut(s) 10, 241, 979
Tsp45I GTSAC 5 cut(s) 338, 471, 875, 896, 1090
TspDTI ATGAA 6 cut(s) 66, 350, 410, 534, 662, 1025
TspRI CASTG 3 cut(s) 272, 862, 955
Van91I CCANNNNNTGG 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 883
XapI RAATTY 1 cut(s) 914
XceI RCATGY 1 cut(s) 460
XcmI CCANNNNNNNNNTGG 2 cut(s) 814, 955
XhoI CTCGAG 1 cut(s) 743
XmnI GAANNNNTTC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.