pycom08g05400

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000799
Physical Location & Seq
Reverse (-)
4133446 .. 4136848
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05400.3

Sequence Viewer

Length: 1602 bp
ATGAGCAACATACCATCAGTTGTTGTGAGAGAGAATGAGGGGGAGATCATCTCTGCAAATGTTGGAGTTGAAGATGTATTTGAATTATTTCCTTTAAGTTTTTTTAATTATGTAACATCCTCTTTCAACTTCTCTAGTCATCTTCTTCTGCATTGCTGCGTTCCTCCTCAACTCCTCCAACCAATATTACACATAAAGAGATCCGAGGAGTTTGGGAAAACCCTAACCCTGATTCAATCCACAATCCCGTCATTAAAACGCATCACTTTGTCCAACTCCCAACTGCTATCAGTTATGGCTGCTGTGGCACCGGAAGCATCCCAATTTGATACTCATCAGTATGATGCAAAAATGAGTGAGTTGTGGTTCATAATTTGTGTTGCCAACGATAAGGATTTATTCTTGGTTTACTGGATTGATGGGAGTAGAGTTGGAGCTGAAGGGCAAGACTTCTTCACATCGTATGATGAGGTTCATGACAGCTTTGACGCTATGGGATTGCAGGAGAACCTTCTTCGGGGCATCTATGCATATGGTTTTGAGAAGCCCTCTGCAATTCAACAAAGGGGAATTGTTCCTTTCAGCAAGGGACTTGATGTGATTCAGCAGGCACAATCTGGAACTGGAAAAACAGCGACTTTCTGCTCTGGGATTTTGCAGCAGCTTGATTATGGCTTACTTGATTGCCAAGCATTGGTTCTTGCACCCACACGAGAGCTTGCTCAACAGATTGAAAAGGTTACGAGAGCACTGGGTGACTATCTTGGTGTCAAGGTCCATGCTTGTGTTGGTGGGACCAGTGTTCGTGAAGATCAACGCATTCTCTCCAGTGGAGTCCATGTCGTTGTTGGCACTCCTGGTCGTGTTTTTGACATGCTACGAAGGCAGTCTCTTTGTTGTGATTACATTAAGATGTTTGTTTTAGATGAAGCAGATGAAATGCTTTCACGAGGTTTCAAGGATCAGATCTATGACATTTTCCAGCTGCTACCACCGAAAATTCAGGTCGGAGTTTTCTCCGCCACAATGCCACCGGAGGCCCTTGATATCACTAGGAAGTTCTTGAACAAACCTGTAAGGATTTTGGTGAAACGTGACGAGCTCACCCTTGAAGGTATCAAGCAGTTTCATGTCAATGTTGAGAAAGAGGATTGGAAACTCGAGACACTTTGTGATCTTTATGAGACCTTGGCAATTACACAGAGTGTCATCTTTGCGAACACCAGGCGCAAAGTTGATTGGTTAACAGACAAGATGCGCAGTCGTGATCACACAGTTTCTGCCACCCACGGAGACATGGACCAAAACACGAGGGATATCATCATGAGAGAGTTTCGGTCTGGTTCTTCTCGTGTGCTCATAACCACTGATCTGTTGGCCCGTGGTATTGACGTCCAACAGGTCTCTCTTGTGATTAATTACGACCTTCCAACACAACCGGAGAACTACCTCCATCGTATTGGTCGTAGTGGACGGTTTGGGAGGAAGGGTGTTGCCATCAATTTTGTCACCAAGGACGACGACAGGACGTTGTATGATATCCAGAAGTTTTATAACGTTGTCATTGAGGAGCTGCCGGCAAATGTTGCTGATCTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

534

Amino Acids

60.15

Weight (kDa)

5.42

Isoelectric Point (pI)

46.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1554
AatII GACGTC 1 cut(s) 1395
Acc16I TGCGCA 1 cut(s) 1259
AccB1I GGYRCC 1 cut(s) 307
AccB7I CCANNNNNTGG 1 cut(s) 694
AciI CCGC 1 cut(s) 1020
AclI AACGTT 1 cut(s) 1557
AclWI GGATC 2 cut(s) 195, 969
AcsI RAATTY 1 cut(s) 999
AcuI CTGAAG 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 1392
AdeI CACNNNGTG 3 cut(s) 755, 1172, 1205
AfiI CCNNNNNNNGG 2 cut(s) 517, 694
AgsI TTSAA 9 cut(s) 71, 83, 127, 236, 560, 734, 958, 1066, 1112
AjnI CCWGG 2 cut(s) 856, 1223
AjuI GAANNNNNNNTTGG 2 cut(s) 681, 713
AluBI AGCT 7 cut(s) 437, 483, 664, 718, 985, 1102, 1573
AluI AGCT 7 cut(s) 437, 483, 664, 718, 985, 1102, 1573
Alw21I GWGCWC 3 cut(s) 751, 1104, 1359
Alw26I GTCTC 5 cut(s) 894, 1157, 1178, 1287, 1408
AlwI GGATC 2 cut(s) 195, 969
AlwNI CAGNNNCTG 1 cut(s) 1280
Ama87I CYCGRG 1 cut(s) 1160
AoxI GGCC 2 cut(s) 1038, 1377
ApeKI GCWGC 6 cut(s) 156, 299, 658, 661, 985, 1573
ApoI RAATTY 1 cut(s) 999
AseI ATTAAT 1 cut(s) 1416
Asp700I GAANNNNTTC 1 cut(s) 87
AspLEI GCGC 2 cut(s) 1230, 1260
AspS9I GGNCC 5 cut(s) 775, 795, 1039, 1300, 1378
AsuHPI GGTGA 4 cut(s) 767, 1096, 1099, 1501
AvaI CYCGRG 1 cut(s) 1160
AvaII GGWCC 3 cut(s) 775, 795, 1300
BanI GGYRCC 1 cut(s) 307
BanII GRGCYC 1 cut(s) 1104
BauI CACGAG 4 cut(s) 711, 948, 1309, 1350
Bbv12I GWGCWC 3 cut(s) 751, 1104, 1359
BbvI GCAGC 6 cut(s) 143, 286, 670, 673, 972, 1560
BccI CCATC 4 cut(s) 22, 413, 1461, 1505
BciT130I CCWGG 2 cut(s) 858, 1225
BclI TGATCA 1 cut(s) 1267
BcoDI GTCTC 5 cut(s) 894, 1157, 1178, 1287, 1408
BfaI CTAG 2 cut(s) 135, 1053
BglII AGATCT 1 cut(s) 966
BisI GCNGC 6 cut(s) 157, 300, 659, 662, 986, 1574
BlsI GCNGC 6 cut(s) 158, 301, 660, 663, 987, 1575
Bme1390I CCNGG 2 cut(s) 858, 1225
Bme18I GGWCC 3 cut(s) 775, 795, 1300
BmeT110I CYCGRG 1 cut(s) 1160
BmgT120I GGNCC 5 cut(s) 775, 795, 1039, 1300, 1378
BmiI GGNNCC 2 cut(s) 309, 796
BmrFI CCNGG 2 cut(s) 858, 1225
BmrI ACTGGG 1 cut(s) 761
BmsI GCATC 5 cut(s) 270, 326, 334, 531, 1245
BmuI ACTGGG 1 cut(s) 761
BplI GAGNNNNNCTC 4 cut(s) 35, 67, 533, 565
BpmI CTGGAG 1 cut(s) 811
BsaBI GATNNNNATC 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 1392
BsaI GGTCTC 2 cut(s) 1178, 1408
BsaJI CCNNGG 5 cut(s) 204, 1188, 1288, 1381, 1512
BsaWI WCCGGW 3 cut(s) 310, 1033, 1438
BsaXI ACNNNNNCTCC 4 cut(s) 825, 855, 1474, 1504
Bsc4I CCNNNNNNNGG 2 cut(s) 517, 694
Bse118I RCCGGY 1 cut(s) 1576
Bse1I ACTGG 5 cut(s) 416, 628, 756, 798, 828
Bse3DI GCAATG 1 cut(s) 151
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 2 cut(s) 858, 1225
BseDI CCNNGG 5 cut(s) 204, 1188, 1288, 1381, 1512
BseGI GGATG 2 cut(s) 116, 317
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 517, 694
BseMI GCAATG 1 cut(s) 151
BseNI ACTGG 5 cut(s) 416, 628, 756, 798, 828
BseRI GAGGAG 4 cut(s) 156, 164, 221, 1583
BseXI GCAGC 6 cut(s) 143, 286, 670, 673, 972, 1560
BshFI GGCC 2 cut(s) 1040, 1379
BshNI GGYRCC 1 cut(s) 307
BsiHKAI GWGCWC 3 cut(s) 751, 1104, 1359
BsiHKCI CYCGRG 1 cut(s) 1160
BsiSI CCGG 4 cut(s) 311, 1034, 1439, 1577
BslFI GGGAC 2 cut(s) 603, 808
BslI CCNNNNNNNGG 2 cut(s) 517, 694
BsmAI GTCTC 5 cut(s) 894, 1157, 1178, 1287, 1408
BsmFI GGGAC 2 cut(s) 603, 808
BsmI GAATGC 1 cut(s) 819
BsnI GGCC 2 cut(s) 1040, 1379
Bso31I GGTCTC 2 cut(s) 1178, 1408
BsoBI CYCGRG 1 cut(s) 1160
Bsp1286I GDGCHC 3 cut(s) 751, 1104, 1359
Bsp143I GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
BspACI CCGC 1 cut(s) 1020
BspANI GGCC 2 cut(s) 1040, 1379
BspHI TCATGA 2 cut(s) 475, 1323
BspLI GGNNCC 2 cut(s) 309, 796
BspPI GGATC 2 cut(s) 195, 969
BspT107I GGYRCC 1 cut(s) 307
BspTNI GGTCTC 2 cut(s) 1178, 1408
BsrDI GCAATG 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 1576
BsrI ACTGG 5 cut(s) 416, 628, 756, 798, 828
BssAI RCCGGY 1 cut(s) 1576
BssECI CCNNGG 5 cut(s) 204, 1188, 1288, 1381, 1512
BssMI GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
BssNI GRCGYC 1 cut(s) 1392
BssSI CACGAG 4 cut(s) 711, 948, 1309, 1350
BssT1I CCWWGG 2 cut(s) 1188, 1512
Bst2BI CACGAG 4 cut(s) 711, 948, 1309, 1350
Bst2UI CCWGG 2 cut(s) 858, 1225
Bst4CI ACNGT 2 cut(s) 1276, 1476
BstACI GRCGYC 1 cut(s) 1392
BstAPI GCANNNNNTGC 1 cut(s) 1586
BstC8I GCNNGC 3 cut(s) 609, 720, 1578
BstDSI CCRYGG 2 cut(s) 1288, 1381
BstF5I GGATG 2 cut(s) 116, 317
BstHHI GCGC 2 cut(s) 1230, 1260
BstKTI GATC 9 cut(s) 48, 203, 814, 964, 969, 1177, 1270, 1372, 1594
BstMAI GTCTC 5 cut(s) 894, 1157, 1178, 1287, 1408
BstMBI GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
BstMWI GCNNNNNNNGC 4 cut(s) 305, 314, 883, 1586
BstNI CCWGG 2 cut(s) 858, 1225
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 2 cut(s) 856, 1223
BstV1I GCAGC 6 cut(s) 143, 286, 670, 673, 972, 1560
BstX2I RGATCY 2 cut(s) 200, 966
BstXI CCANNNNNNTGG 1 cut(s) 1460
BstYI RGATCY 2 cut(s) 200, 966
BsuRI GGCC 2 cut(s) 1040, 1379
BtgI CCRYGG 2 cut(s) 1288, 1381
BtsCI GGATG 2 cut(s) 116, 317
BtsIMutI CAGTG 4 cut(s) 749, 805, 835, 1365
Cac8I GCNNGC 3 cut(s) 609, 720, 1578
CaiI CAGNNNCTG 1 cut(s) 1280
CciI TCATGA 2 cut(s) 475, 1323
CfoI GCGC 2 cut(s) 1230, 1260
Cfr10I RCCGGY 1 cut(s) 1576
Cfr13I GGNCC 5 cut(s) 775, 795, 1039, 1300, 1378
CseI GACGC 1 cut(s) 497
CviAII CATG 7 cut(s) 476, 779, 839, 874, 1130, 1297, 1324
DpnI GATC 9 cut(s) 47, 202, 813, 963, 968, 1176, 1269, 1371, 1593
DpnII GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
DraIII CACNNNGTG 3 cut(s) 755, 1172, 1205
EciI GGCGGA 1 cut(s) 1009
Ecl136II GAGCTC 1 cut(s) 1102
Eco130I CCWWGG 2 cut(s) 1188, 1512
Eco24I GRGCYC 1 cut(s) 1104
Eco31I GGTCTC 2 cut(s) 1178, 1408
Eco32I GATATC 3 cut(s) 1048, 1318, 1540
Eco47I GGWCC 3 cut(s) 775, 795, 1300
Eco53kI GAGCTC 1 cut(s) 1102
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 1160
EcoICRI GAGCTC 1 cut(s) 1102
EcoO109I RGGNCCY 1 cut(s) 1039
EcoRII CCWGG 2 cut(s) 856, 1223
EcoRV GATATC 3 cut(s) 1048, 1318, 1540
EcoT14I CCWWGG 2 cut(s) 1188, 1512
EcoT22I ATGCAT 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 1104
ErhI CCWWGG 2 cut(s) 1188, 1512
FaeI CATG 7 cut(s) 479, 782, 842, 877, 1133, 1300, 1327
FaqI GGGAC 2 cut(s) 603, 808
FatI CATG 7 cut(s) 475, 778, 838, 873, 1129, 1296, 1323
FauNDI CATATG 1 cut(s) 532
FbaI TGATCA 1 cut(s) 1267
Fnu4HI GCNGC 6 cut(s) 157, 300, 659, 662, 986, 1574
FokI GGATG 2 cut(s) 103, 304
FriOI GRGCYC 1 cut(s) 1104
Fsp4HI GCNGC 6 cut(s) 157, 300, 659, 662, 986, 1574
FspBI CTAG 2 cut(s) 135, 1053
FspI TGCGCA 1 cut(s) 1259
GlaI GCGC 2 cut(s) 1229, 1259
GluI GCNGC 6 cut(s) 157, 300, 659, 662, 986, 1574
GsuI CTGGAG 1 cut(s) 811
HaeIII GGCC 2 cut(s) 1040, 1379
HapII CCGG 4 cut(s) 311, 1034, 1439, 1577
HgaI GACGC 1 cut(s) 497
HhaI GCGC 2 cut(s) 1230, 1260
Hin1I GRCGYC 1 cut(s) 1392
Hin1II CATG 7 cut(s) 479, 782, 842, 877, 1133, 1300, 1327
Hin6I GCGC 2 cut(s) 1228, 1258
HinP1I GCGC 2 cut(s) 1228, 1258
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HinfI GANTC 3 cut(s) 232, 601, 834
HpaI GTTAAC 1 cut(s) 1245
HpaII CCGG 4 cut(s) 311, 1034, 1439, 1577
HphI GGTGA 4 cut(s) 767, 1096, 1099, 1501
Hpy166II GTNNAC 3 cut(s) 409, 1245, 1472
Hpy188I TCNGA 4 cut(s) 205, 966, 1010, 1601
Hpy188III TCNNGA 9 cut(s) 476, 618, 806, 948, 1063, 1162, 1265, 1324, 1543
Hpy8I GTNNAC 3 cut(s) 409, 1245, 1472
Hpy99I CGWCG 1 cut(s) 1523
HpyAV CCTTC 6 cut(s) 434, 521, 876, 1106, 1436, 1480
HpyCH4III ACNGT 2 cut(s) 1276, 1476
HpyCH4IV ACGT 4 cut(s) 1093, 1392, 1529, 1557
HpyCH4V TGCA 8 cut(s) 56, 151, 347, 502, 530, 554, 658, 704
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 314, 883, 1586
HpySE526I ACGT 4 cut(s) 1093, 1392, 1529, 1557
Hsp92I GRCGYC 1 cut(s) 1392
Hsp92II CATG 7 cut(s) 479, 782, 842, 877, 1133, 1300, 1327
HspAI GCGC 2 cut(s) 1228, 1258
KroI GCCGGC 1 cut(s) 1576
KroNI GCCGGC 1 cut(s) 1578
Ksp22I TGATCA 1 cut(s) 1267
KspAI GTTAAC 1 cut(s) 1245
Kzo9I GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
LmnI GCTCC 2 cut(s) 434, 1570
Lsp1109I GCAGC 6 cut(s) 143, 286, 670, 673, 972, 1560
LweI GCATC 5 cut(s) 270, 326, 334, 531, 1245
MaeI CTAG 2 cut(s) 135, 1053
MaeII ACGT 4 cut(s) 1093, 1392, 1529, 1557
MaeIII GTNAC 5 cut(s) 112, 739, 755, 1094, 1507
MalI GATC 9 cut(s) 47, 202, 813, 963, 968, 1176, 1269, 1371, 1593
MboI GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
MboII GAAGA 7 cut(s) 83, 134, 137, 445, 506, 821, 1338
MflI RGATCY 2 cut(s) 200, 966
MhlI GDGCHC 3 cut(s) 751, 1104, 1359
MlyI GAGTC 1 cut(s) 843
MmeI TCCRAC 7 cut(s) 43, 202, 297, 412, 988, 1420, 1454
Mph1103I ATGCAT 1 cut(s) 532
MroNI GCCGGC 1 cut(s) 1576
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 6 cut(s) 95, 105, 254, 909, 1244, 1416
MslI CAYNNNNRTG 4 cut(s) 339, 783, 911, 1134
MspA1I CMGCKG 1 cut(s) 985
MspI CCGG 4 cut(s) 311, 1034, 1439, 1577
MspR9I CCNGG 2 cut(s) 858, 1225
Mva1269I GAATGC 1 cut(s) 819
MvaI CCWGG 2 cut(s) 858, 1225
MwoI GCNNNNNNNGC 4 cut(s) 305, 314, 883, 1586
NaeI GCCGGC 1 cut(s) 1578
NdeI CATATG 1 cut(s) 532
NdeII GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
NgoMIV GCCGGC 1 cut(s) 1576
NlaIII CATG 7 cut(s) 479, 782, 842, 877, 1133, 1300, 1327
NlaIV GGNNCC 2 cut(s) 309, 796
NmuCI GTSAC 3 cut(s) 755, 1094, 1507
NsbI TGCGCA 1 cut(s) 1259
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 1 cut(s) 877
PaeR7I CTCGAG 1 cut(s) 1160
PagI TCATGA 2 cut(s) 475, 1323
PcsI WCGNNNNNNNCGW 1 cut(s) 1462
PctI GAATGC 1 cut(s) 819
PdiI GCCGGC 1 cut(s) 1578
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 2 cut(s) 232, 601
PflMI CCANNNNNTGG 1 cut(s) 694
PkrI GCNGC 6 cut(s) 158, 301, 660, 663, 987, 1575
PleI GAGTC 1 cut(s) 842
PpsI GAGTC 1 cut(s) 842
PshBI ATTAAT 1 cut(s) 1416
PsiI TTATAA 1 cut(s) 1554
Psp124BI GAGCTC 1 cut(s) 1104
Psp1406I AACGTT 1 cut(s) 1557
Psp6I CCWGG 2 cut(s) 856, 1223
PspGI CCWGG 2 cut(s) 856, 1223
PspN4I GGNNCC 2 cut(s) 309, 796
PspPI GGNCC 5 cut(s) 775, 795, 1039, 1300, 1378
PstNI CAGNNNCTG 1 cut(s) 1280
PsuI RGATCY 2 cut(s) 200, 966
PvuII CAGCTG 1 cut(s) 985
RseI CAYNNNNRTG 4 cut(s) 339, 783, 911, 1134
SacI GAGCTC 1 cut(s) 1104
SaqAI TTAA 6 cut(s) 95, 105, 254, 909, 1244, 1416
SatI GCNGC 6 cut(s) 157, 300, 659, 662, 986, 1574
Sau3AI GATC 9 cut(s) 45, 200, 811, 961, 966, 1174, 1267, 1369, 1591
Sau96I GGNCC 5 cut(s) 775, 795, 1039, 1300, 1378
SchI GAGTC 1 cut(s) 843
ScrFI CCNGG 2 cut(s) 858, 1225
SduI GDGCHC 3 cut(s) 751, 1104, 1359
SfaNI GCATC 5 cut(s) 270, 326, 334, 531, 1245
Sfr274I CTCGAG 1 cut(s) 1160
SinI GGWCC 3 cut(s) 775, 795, 1300
SlaI CTCGAG 1 cut(s) 1160
SmiMI CAYNNNNRTG 4 cut(s) 339, 783, 911, 1134
SmlI CTYRAG 1 cut(s) 1160
SmoI CTYRAG 1 cut(s) 1160
SsiI CCGC 1 cut(s) 1020
SspI AATATT 1 cut(s) 186
SspMI CTAG 2 cut(s) 135, 1053
SstI GAGCTC 1 cut(s) 1104
StyD4I CCNGG 2 cut(s) 856, 1223
StyI CCWWGG 2 cut(s) 1188, 1512
TaaI ACNGT 2 cut(s) 1276, 1476
TaiI ACGT 4 cut(s) 1096, 1395, 1532, 1560
TaqI TCGA 1 cut(s) 1161
TaqII GACCGA 1 cut(s) 1326
TfiI GAWTC 2 cut(s) 232, 601
Tru1I TTAA 6 cut(s) 95, 105, 254, 909, 1244, 1416
Tru9I TTAA 6 cut(s) 95, 105, 254, 909, 1244, 1416
TscAI CASTG 4 cut(s) 756, 805, 835, 1372
TseFI GTSAC 3 cut(s) 755, 1094, 1507
TseI GCWGC 6 cut(s) 156, 299, 658, 661, 985, 1573
Tsp45I GTSAC 3 cut(s) 755, 1094, 1507
TspDTI ATGAA 5 cut(s) 358, 464, 942, 951, 1118
TspGWI ACGGA 1 cut(s) 1305
TspRI CASTG 4 cut(s) 756, 805, 835, 1372
Van91I CCANNNNNTGG 1 cut(s) 694
VpaK11BI GGWCC 3 cut(s) 775, 795, 1300
VspI ATTAAT 1 cut(s) 1416
XapI RAATTY 1 cut(s) 999
XceI RCATGY 1 cut(s) 877
XcmI CCANNNNNNNNNTGG 3 cut(s) 785, 845, 1372
XhoI CTCGAG 1 cut(s) 1160
XmnI GAANNNNTTC 1 cut(s) 87
XspI CTAG 2 cut(s) 135, 1053
ZraI GACGTC 1 cut(s) 1393
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.