MD02G1082700.v1.1

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000799
Physical Location & Seq
Reverse (-)
6471186 .. 6474046
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1082700.v1.1.491

Sequence Viewer

Length: 1524 bp
ATGGGTACCCGCCACGTACCCGGATGGAATCTGGACTGGAGGGACGCGAAGACCATACACAAACCCTCACTCGCTGAGTTTTCACTCATCAATCATCATCCGCCCAACCCCTCTCTCTCTCTCTCGCTTTCTCCCTCGCGCAATCCTCAACTCCGCGGCTTAGATCCGAGGAGTTTGGGAAACCCTAATTTCCAGAAAACCAGCACAAAGCTCTCTTCCGACGCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCGCCTTCAACTTTCTCACTCATCACTCGCAGTCATGGCAGCAGCACCTGAAGGATCGCAATTTGACACTCGTCAGTATGATACCAAAATGAGCGAAATACTTGCAACTGATCAGCAGGAATTCTTTACATCGTATGATGAAGTTTATGATAGTTTTGATGCTATGGGTCTTCAAGAGAATCTTCTGAGGGGAATCTATGCTTACGGTTTTGAAAAGCCCTCGGCTATTCAGCAAAGAGGAATTGTTCCCTTCTGCAAGGGTCTTGATGTGATTCAACAGGCTCAGTCTGGAACTGGAAAGACAGCGACTTTCTGTTCTGGTATTCTTCAACAGCTTGATTATAGTTTGACGGAATGCCAAGCCTTGGTTCTTGCACCCACTCGAGAGCTTGCTCAACAGATTGAGAAGGTTATGAGGGCTCTTGGAGATTATCTAGGTGTGAGGGTGCATGCTTGTGTTGGTGGAACTAGTGTCCGTGAGGACCAACGCATTCTGTCAAATGGAGTTCATGTTGTTGTTGGAACTCCAGGTCGTGTTTTTGACATGCTTAGAAGGCAGTCACTTCGTCCTGATAGCATCAAGATGTTTGTTTTGGATGAGGCTGATGAAATGCTTTCCCGTGGTTTCAAGGATCAGATCTATGACATATTCCAGCTTCTTCCATCAAAGATTCAGGTTGGTGTGTTCTCTGCCACAATGCCACCTGAGGCCCTCGAGATCACAAGGAAGTTCATGAACAAACCCGTGAGGATTCTTGTGAAGCGTGATGAACTCACCCTGGAAGGTATAAAGCAATTCTATGTCAATGTCGACAAGGAGGAATGGAAGCTTGAGACTCTTTGCGATCTGTATGAGACCTTGGCTATTACCCAGAGTGTCATTTTTGTTAATACTCGACGCAAGGTTGACTGGTTGACTGACAAGATGAGGAGCCGAGACCATACAGTTTCTGCCACCCATGGAGACATGGACCAGAACACCAGAGACATCATCATGCGAGAGTTTCGTTCTGGATCATCTCGTGTCTTGATCACCACTGATCTTTTGGCTCGTGGTATTGATGTTCAGCAAGTCTCCCTAGTTATTAACTATGATCTGCCCACCCAGCCCGAGAACTACCTTCACAGAATTGGTCGTAGTGGACGTTTTGGAAGGAAGGGTGTTGCAATCAACTTCGTAACAAAGGATGATGAGAGGATGCTGTTTGACATCCAGAAGTTCTATAACGTGGTTGTGGAGGAGCTGCCATCAAACGTCGCGGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C WD40

Protein Analysis

508

Amino Acids

57.24

Weight (kDa)

6.4

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 159 - 321 1.2e-43 DEAD/DEAH box helicase
ResIII PF04851 177 - 317 2.4e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 360 - 468 1.2e-31 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 5
AccB7I CCANNNNNTGG 1 cut(s) 616
AccI GTMKAC 1 cut(s) 1062
AccII CGCG 4 cut(s) 47, 139, 156, 1511
AciI CCGC 5 cut(s) 10, 101, 154, 156, 1511
AclWI GGATC 5 cut(s) 158, 313, 891, 1273, 1521
AcsI RAATTY 1 cut(s) 371
AcuI CTGAAG 1 cut(s) 321
AfaI GTAC 2 cut(s) 7, 18
AfiI CCNNNNNNNGG 1 cut(s) 616
AgsI TTSAA 6 cut(s) 260, 425, 464, 527, 581, 880
AhlI ACTAGT 1 cut(s) 719
AjnI CCWGG 2 cut(s) 778, 1029
AjuI GAANNNNNNNTTGG 2 cut(s) 603, 635
AluBI AGCT 6 cut(s) 211, 586, 640, 907, 1081, 1495
AluI AGCT 6 cut(s) 211, 586, 640, 907, 1081, 1495
Alw26I GTCTC 6 cut(s) 1079, 1100, 1182, 1209, 1230, 1330
AlwI GGATC 5 cut(s) 158, 313, 891, 1273, 1521
AlwNI CAGNNNCTG 2 cut(s) 299, 1202
Ama87I CYCGRG 3 cut(s) 633, 965, 1361
AoxI GGCC 1 cut(s) 960
ApeKI GCWGC 3 cut(s) 290, 293, 1495
ApoI RAATTY 1 cut(s) 371
Asp718I GGTACC 1 cut(s) 5
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 3 cut(s) 733, 961, 1222
AsuC2I CCSGG 1 cut(s) 21
AsuHPI GGTGA 2 cut(s) 1018, 1276
AvaI CYCGRG 3 cut(s) 633, 965, 1361
AvaII GGWCC 2 cut(s) 733, 1222
AxyI CCTNAGG 1 cut(s) 957
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 1 cut(s) 673
BauI CACGAG 2 cut(s) 1272, 1302
BbsI GAAGAC 2 cut(s) 56, 413
BbvI GCAGC 3 cut(s) 302, 305, 1482
BccI CCATC 3 cut(s) 18, 922, 1507
BcgI CGANNNNNNTGC 4 cut(s) 335, 369, 797, 831
BciT130I CCWGG 2 cut(s) 780, 1031
BclI TGATCA 2 cut(s) 361, 1281
BcnI CCSGG 1 cut(s) 21
BcoDI GTCTC 6 cut(s) 1079, 1100, 1182, 1209, 1230, 1330
BcuI ACTAGT 1 cut(s) 719
BfaI CTAG 3 cut(s) 686, 720, 1331
BglII AGATCT 1 cut(s) 888
BisI GCNGC 4 cut(s) 157, 291, 294, 1496
BlsI GCNGC 4 cut(s) 158, 292, 295, 1497
Bme1390I CCNGG 3 cut(s) 21, 780, 1031
Bme18I GGWCC 2 cut(s) 733, 1222
BmeT110I CYCGRG 3 cut(s) 633, 965, 1361
BmgT120I GGNCC 3 cut(s) 733, 961, 1222
BmiI GGNNCC 2 cut(s) 7, 1184
BmrFI CCNGG 3 cut(s) 21, 780, 1031
BmsI GCATC 3 cut(s) 400, 837, 1440
BoxI GACNNNNGTC 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 56, 413
BpmI CTGGAG 2 cut(s) 58, 762
BpuEI CTTGAG 1 cut(s) 1103
BpuMI CCSGG 1 cut(s) 21
BsaAI YACGTR 1 cut(s) 16
BsaI GGTCTC 2 cut(s) 1100, 1182
BsaJI CCNNGG 8 cut(s) 154, 167, 471, 615, 871, 1029, 1110, 1210
BsaXI ACNNNNNCTCC 2 cut(s) 747, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse1I ACTGG 3 cut(s) 41, 550, 1166
Bse21I CCTNAGG 1 cut(s) 957
BseBI CCWGG 2 cut(s) 780, 1031
BseDI CCNNGG 8 cut(s) 154, 167, 471, 615, 871, 1029, 1110, 1210
BseGI GGATG 6 cut(s) 29, 97, 853, 1444, 1455, 1461
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMII CTCAG 4 cut(s) 66, 428, 548, 948
BseNI ACTGG 3 cut(s) 41, 550, 1166
BseRI GAGGAG 4 cut(s) 184, 240, 1195, 1505
BseXI GCAGC 3 cut(s) 302, 305, 1482
BseYI CCCAGC 1 cut(s) 1356
Bsh1236I CGCG 4 cut(s) 47, 139, 156, 1511
BshFI GGCC 1 cut(s) 962
BshNI GGYRCC 1 cut(s) 5
BsiHKCI CYCGRG 3 cut(s) 633, 965, 1361
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 6 cut(s) 1079, 1100, 1182, 1209, 1230, 1330
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 2 cut(s) 611, 741
BsnI GGCC 1 cut(s) 962
Bso31I GGTCTC 2 cut(s) 1100, 1182
BsoBI CYCGRG 3 cut(s) 633, 965, 1361
Bsp1286I GDGCHC 1 cut(s) 673
Bsp19I CCATGG 1 cut(s) 1210
BspACI CCGC 5 cut(s) 10, 101, 154, 156, 1511
BspANI GGCC 1 cut(s) 962
BspCNI CTCAG 4 cut(s) 67, 429, 547, 949
BspFNI CGCG 4 cut(s) 47, 139, 156, 1511
BspHI TCATGA 1 cut(s) 984
BspLI GGNNCC 2 cut(s) 7, 1184
BspPI GGATC 5 cut(s) 158, 313, 891, 1273, 1521
BspT107I GGYRCC 1 cut(s) 5
BspTNI GGTCTC 2 cut(s) 1100, 1182
BsrI ACTGG 3 cut(s) 41, 550, 1166
BssECI CCNNGG 8 cut(s) 154, 167, 471, 615, 871, 1029, 1110, 1210
BssSI CACGAG 2 cut(s) 1272, 1302
BssT1I CCWWGG 3 cut(s) 615, 1110, 1210
Bst2BI CACGAG 2 cut(s) 1272, 1302
Bst2UI CCWGG 2 cut(s) 780, 1031
Bst4CI ACNGT 2 cut(s) 458, 1198
Bst6I CTCTTC 1 cut(s) 220
BstBAI YACGTR 1 cut(s) 16
BstC8I GCNNGC 2 cut(s) 642, 702
BstDEI CTNAG 6 cut(s) 75, 160, 437, 534, 800, 957
BstDSI CCRYGG 3 cut(s) 154, 871, 1210
BstF5I GGATG 6 cut(s) 29, 97, 853, 1444, 1455, 1461
BstFNI CGCG 4 cut(s) 47, 139, 156, 1511
BstHHI GCGC 1 cut(s) 141
BstMAI GTCTC 6 cut(s) 1079, 1100, 1182, 1209, 1230, 1330
BstMWI GCNNNNNNNGC 3 cut(s) 287, 805, 1357
BstNI CCWGG 2 cut(s) 780, 1031
BstNSI RCATGY 2 cut(s) 704, 799
BstPAI GACNNNNGTC 1 cut(s) 321
BstSCI CCNGG 3 cut(s) 19, 778, 1029
BstUI CGCG 4 cut(s) 47, 139, 156, 1511
BstV1I GCAGC 3 cut(s) 302, 305, 1482
BstV2I GAAGAC 2 cut(s) 56, 413
BstX2I RGATCY 3 cut(s) 163, 888, 1513
BstYI RGATCY 3 cut(s) 163, 888, 1513
Bsu36I CCTNAGG 1 cut(s) 957
BsuRI GGCC 1 cut(s) 962
BtgI CCRYGG 3 cut(s) 154, 871, 1210
BtsCI GGATG 6 cut(s) 29, 97, 853, 1444, 1455, 1461
BtsIMutI CAGTG 1 cut(s) 1287
Cac8I GCNNGC 2 cut(s) 642, 702
CaiI CAGNNNCTG 2 cut(s) 299, 1202
CciI TCATGA 1 cut(s) 984
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 3 cut(s) 733, 961, 1222
Cfr42I CCGCGG 1 cut(s) 157
CseI GACGC 3 cut(s) 53, 230, 1158
Csp6I GTAC 2 cut(s) 6, 17
CviAII CATG 8 cut(s) 286, 701, 761, 796, 985, 1211, 1219, 1246
CviQI GTAC 2 cut(s) 6, 17
DdeI CTNAG 6 cut(s) 75, 160, 437, 534, 800, 957
Eam1104I CTCTTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 220
EciI GGCGGA 1 cut(s) 90
Eco130I CCWWGG 3 cut(s) 615, 1110, 1210
Eco24I GRGCYC 1 cut(s) 673
Eco31I GGTCTC 2 cut(s) 1100, 1182
Eco47I GGWCC 2 cut(s) 733, 1222
Eco57I CTGAAG 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 957
Eco88I CYCGRG 3 cut(s) 633, 965, 1361
EcoO109I RGGNCCY 1 cut(s) 961
EcoRI GAATTC 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 778, 1029
EcoT14I CCWWGG 3 cut(s) 615, 1110, 1210
EcoT38I GRGCYC 1 cut(s) 673
ErhI CCWWGG 3 cut(s) 615, 1110, 1210
FaeI CATG 8 cut(s) 289, 704, 764, 799, 988, 1214, 1222, 1249
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 1 cut(s) 56
FatI CATG 8 cut(s) 285, 700, 760, 795, 984, 1210, 1218, 1245
FauI CCCGC 1 cut(s) 17
FbaI TGATCA 2 cut(s) 361, 1281
FblI GTMKAC 1 cut(s) 1062
Fnu4HI GCNGC 4 cut(s) 157, 291, 294, 1496
FokI GGATG 6 cut(s) 36, 84, 860, 1448, 1451, 1462
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 4 cut(s) 157, 291, 294, 1496
FspBI CTAG 3 cut(s) 686, 720, 1331
GlaI GCGC 1 cut(s) 140
GluI GCNGC 4 cut(s) 157, 291, 294, 1496
GsaI CCCAGC 1 cut(s) 1360
GsuI CTGGAG 2 cut(s) 58, 762
HaeIII GGCC 1 cut(s) 962
HapII CCGG 1 cut(s) 21
HgaI GACGC 3 cut(s) 53, 230, 1158
HhaI GCGC 1 cut(s) 141
Hin1II CATG 8 cut(s) 289, 704, 764, 799, 988, 1214, 1222, 1249
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HincII GTYRAC 3 cut(s) 1063, 1159, 1167
HindII GTYRAC 3 cut(s) 1063, 1159, 1167
HindIII AAGCTT 1 cut(s) 1079
HinfI GANTC 7 cut(s) 28, 430, 444, 523, 922, 1003, 1087
HpaII CCGG 1 cut(s) 21
HphI GGTGA 2 cut(s) 1018, 1276
Hpy166II GTNNAC 4 cut(s) 1063, 1159, 1167, 1394
Hpy188I TCNGA 4 cut(s) 168, 220, 438, 888
Hpy8I GTNNAC 4 cut(s) 1063, 1159, 1167, 1394
Hpy99I CGWCG 3 cut(s) 224, 1152, 1511
HpyAV CCTTC 9 cut(s) 266, 296, 511, 652, 798, 1028, 1382, 1398, 1402
HpyCH4III ACNGT 2 cut(s) 458, 1198
HpyCH4IV ACGT 4 cut(s) 15, 1396, 1479, 1506
HpyCH4V TGCA 5 cut(s) 356, 507, 626, 700, 1418
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 805, 1357
HpyF3I CTNAG 6 cut(s) 75, 160, 437, 534, 800, 957
HpySE526I ACGT 4 cut(s) 15, 1396, 1479, 1506
Hsp92II CATG 8 cut(s) 289, 704, 764, 799, 988, 1214, 1222, 1249
HspAI GCGC 1 cut(s) 139
KpnI GGTACC 1 cut(s) 9
Ksp22I TGATCA 2 cut(s) 361, 1281
KspI CCGCGG 1 cut(s) 157
LmnI GCTCC 2 cut(s) 1182, 1492
Lsp1109I GCAGC 3 cut(s) 302, 305, 1482
LweI GCATC 3 cut(s) 400, 837, 1440
MaeI CTAG 3 cut(s) 686, 720, 1331
MaeII ACGT 4 cut(s) 15, 1396, 1479, 1506
MaeIII GTNAC 2 cut(s) 810, 1429
MboII GAAGA 7 cut(s) 61, 207, 413, 425, 569, 902, 1508
MflI RGATCY 3 cut(s) 163, 888, 1513
MhlI GDGCHC 1 cut(s) 673
MluCI AATT 6 cut(s) 187, 311, 371, 492, 1046, 1380
MlyI GAGTC 1 cut(s) 1081
MmeI TCCRAC 2 cut(s) 243, 751
MseI TTAA 2 cut(s) 1140, 1338
MslI CAYNNNNRTG 3 cut(s) 705, 833, 1244
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 3 cut(s) 21, 780, 1031
Mva1269I GAATGC 2 cut(s) 611, 741
MvaI CCWGG 2 cut(s) 780, 1031
MvnI CGCG 4 cut(s) 47, 139, 156, 1511
MwoI GCNNNNNNNGC 3 cut(s) 287, 805, 1357
NciI CCSGG 1 cut(s) 21
NcoI CCATGG 1 cut(s) 1210
NlaIII CATG 8 cut(s) 289, 704, 764, 799, 988, 1214, 1222, 1249
NlaIV GGNNCC 2 cut(s) 7, 1184
NmeAIII GCCGAG 2 cut(s) 452, 1211
NmuCI GTSAC 1 cut(s) 810
NspI RCATGY 2 cut(s) 704, 799
PaeI GCATGC 1 cut(s) 704
PaeR7I CTCGAG 2 cut(s) 633, 965
PagI TCATGA 1 cut(s) 984
PcsI WCGNNNNNNNCGW 1 cut(s) 1393
PctI GAATGC 2 cut(s) 611, 741
PfeI GAWTC 6 cut(s) 28, 430, 444, 523, 922, 1003
PflMI CCANNNNNTGG 1 cut(s) 616
PkrI GCNGC 4 cut(s) 158, 292, 295, 1497
PleI GAGTC 1 cut(s) 1081
PpsI GAGTC 1 cut(s) 1081
Ppu21I YACGTR 1 cut(s) 16
PshAI GACNNNNGTC 1 cut(s) 321
Psp6I CCWGG 2 cut(s) 778, 1029
PspFI CCCAGC 1 cut(s) 1356
PspGI CCWGG 2 cut(s) 778, 1029
PspN4I GGNNCC 2 cut(s) 7, 1184
PspPI GGNCC 3 cut(s) 733, 961, 1222
PstNI CAGNNNCTG 2 cut(s) 299, 1202
PsuI RGATCY 3 cut(s) 163, 888, 1513
RsaI GTAC 2 cut(s) 7, 18
RsaNI GTAC 2 cut(s) 6, 17
RseI CAYNNNNRTG 3 cut(s) 705, 833, 1244
SacII CCGCGG 1 cut(s) 157
SalI GTCGAC 1 cut(s) 1061
SaqAI TTAA 2 cut(s) 1140, 1338
SatI GCNGC 4 cut(s) 157, 291, 294, 1496
Sau96I GGNCC 3 cut(s) 733, 961, 1222
SchI GAGTC 1 cut(s) 1081
ScrFI CCNGG 3 cut(s) 21, 780, 1031
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 3 cut(s) 400, 837, 1440
Sfr274I CTCGAG 2 cut(s) 633, 965
Sfr303I CCGCGG 1 cut(s) 157
SgrBI CCGCGG 1 cut(s) 157
SinI GGWCC 2 cut(s) 733, 1222
SlaI CTCGAG 2 cut(s) 633, 965
SmiMI CAYNNNNRTG 3 cut(s) 705, 833, 1244
SmlI CTYRAG 3 cut(s) 633, 965, 1082
SmoI CTYRAG 3 cut(s) 633, 965, 1082
SpeI ACTAGT 1 cut(s) 719
SphI GCATGC 1 cut(s) 704
Sse9I AATT 6 cut(s) 187, 311, 371, 492, 1046, 1380
SsiI CCGC 5 cut(s) 10, 101, 154, 156, 1511
SspMI CTAG 3 cut(s) 686, 720, 1331
StyD4I CCNGG 3 cut(s) 19, 778, 1029
StyI CCWWGG 3 cut(s) 615, 1110, 1210
TaaI ACNGT 2 cut(s) 458, 1198
TaiI ACGT 4 cut(s) 18, 1399, 1482, 1509
TaqI TCGA 4 cut(s) 634, 966, 1062, 1147
TasI AATT 6 cut(s) 187, 311, 371, 492, 1046, 1380
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 6 cut(s) 28, 430, 444, 523, 922, 1003
Tru1I TTAA 2 cut(s) 1140, 1338
Tru9I TTAA 2 cut(s) 1140, 1338
TscAI CASTG 1 cut(s) 1294
TseFI GTSAC 1 cut(s) 810
TseI GCWGC 3 cut(s) 290, 293, 1495
Tsp45I GTSAC 1 cut(s) 810
TspDTI ATGAA 6 cut(s) 405, 749, 873, 973, 1001, 1035
TspGWI ACGGA 2 cut(s) 617, 716
TspRI CASTG 1 cut(s) 1294
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 2 cut(s) 733, 1222
XapI RAATTY 1 cut(s) 371
XceI RCATGY 2 cut(s) 704, 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 1294
XhoI CTCGAG 2 cut(s) 633, 965
XmiI GTMKAC 1 cut(s) 1062
XspI CTAG 3 cut(s) 686, 720, 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.