RLG00000020079

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000799
Physical Location & Seq
Reverse (-)
60794395 .. 60797304
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020079

Sequence Viewer

Length: 1308 bp
ATGAAATTTGCTCTCTCTATCTCTCTTTGCTTTTCTCCGTCAAGCTTTTCATCAACTCAATCAGATATGGCAGGTCTTGCTCCGGAAGGTGCACAGTTTGATGCTAAACAGTATGATTCTAAGATGAATGAGATTCTCTCATCTGAAGGCCAAGAGTTCTTCACATCGTATGATGAGGTTCATGACAGTTTTGATGCCATGGGCTTACAAGAAAATCTTTTAAGGGGAATATATGCTTATGGATTTGAGAAGCCTTCTGCGATTCAGCAAAGAGGAATTGTTCCTTTCTGTAAAGGTTTGGATGTGATTCAACAAGCTCAGTCAGGAACAGGCAAAACAGCAACCTTTTGCTCTGGAATTTTGCAGCAACTTGACTATGGTTTGGTCCAGTGCCAGGCTTTGGTGTTGGCACCTACCAGGGAGTTAGCTCAGCAGATTGAGAAGGTTATGCGGGCACTGGGAGACTATCTCGGCGTGAAGGTTCATGCTTGCGTAGGTGGGACTAGTGTTCGTGAGGATCAGCGAATTCTTCAGACTGGTGTGCATGTGGTGGTTGGCACTCCTGGACGTGTGTTTGACATGTTGCGGAGGCAATCTCTTCGTGCAGATTACATTAAGATGTTTGTATTGGATGAAGCCGATGAAATGCTATCTAGGGGTTTCAAGGATCAGATCTATGACATTTTTCAGCTTCTGCCTTCAAAAGTTCAAGTTGGTGTGTTCTCTGCTACCATGCCTCCTGAAGCCCTTGAGATCACCAGAAAGTTTATGAACAAACCTGTTAGGATTTTGGTTAAGCGTGATGAGCTCACCCTTGAGGGAATCAAGCAATTTTATGTTAATGTCGATAAGGAAGAATGGAAGCTTGAGACACTTTGCGATCTCTATGAGACCCTTGCAATCACTCAGAGTGTTATTTTTGTTAATACAAGGCGCAAAGTGGATTGGCTTACAGATAAGATGCGAAGCCGTGACCACACTGTGTCTGCTACCCATGGTGATATGGACCAGAACCAGCGTGACATTATCATGCGTGAATTCCGATCTGGTTCCTCCCGTGTTCTCATCACTACTGATCTGTTGGCACGTGGTATTGATGTGCAGCAAGTGTCGCTTGTAATTAATTATGATTTGCCGACTCAACCAGAAAACTACCTTCATCGTATTGGACGAAGTGGGCGATTTGGAAGAAAAGGTGTTGCCATCAATTTCGTGACTAGGGATGACGATAGAATGCTATATGACATTCAGAGGTTTTATAATGTGGTGATTGAGGAGCTGCCTTCCAACGTGGCCGATCTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

436

Amino Acids

49.26

Weight (kDa)

5.56

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 87 - 249 1.9e-43 DEAD/DEAH box helicase
ResIII PF04851 105 - 245 9e-07 Type III restriction enzyme, res subunit
Helicase_C PF00271 288 - 396 3.2e-32 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1260
Acc36I ACCTGC 1 cut(s) 62
AccB1I GGYRCC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 400
AccIII TCCGGA 1 cut(s) 82
AciI CCGC 2 cut(s) 451, 586
AclWI GGATC 2 cut(s) 525, 675
AcoI YGGCCR 1 cut(s) 1293
AcsI RAATTY 4 cut(s) 5, 357, 525, 1037
AcuI CTGAAG 3 cut(s) 165, 515, 762
AcvI CACGTG 1 cut(s) 1088
AdeI CACNNNGTG 1 cut(s) 982
AfiI CCNNNNNNNGG 2 cut(s) 394, 400
AflIII ACRYGT 2 cut(s) 568, 579
AgsI TTSAA 4 cut(s) 311, 664, 702, 710
AhlI ACTAGT 1 cut(s) 503
AjiI CACGTC 1 cut(s) 569
AjnI CCWGG 3 cut(s) 393, 416, 562
AluBI AGCT 7 cut(s) 45, 317, 428, 691, 808, 865, 1279
AluI AGCT 7 cut(s) 45, 317, 428, 691, 808, 865, 1279
Alw21I GWGCWC 2 cut(s) 94, 810
Alw26I GTCTC 3 cut(s) 456, 863, 884
Alw44I GTGCAC 1 cut(s) 90
AlwI GGATC 2 cut(s) 525, 675
AlwNI CAGNNNCTG 1 cut(s) 694
Aor13HI TCCGGA 1 cut(s) 82
AoxI GGCC 2 cut(s) 148, 1293
ApaLI GTGCAC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 364, 1102, 1279
ApoI RAATTY 4 cut(s) 5, 357, 525, 1037
AseI ATTAAT 1 cut(s) 1122
AspLEI GCGC 1 cut(s) 936
AspS9I GGNCC 2 cut(s) 385, 1006
AsuHPI GGTGA 4 cut(s) 748, 802, 1010, 1279
AvaII GGWCC 2 cut(s) 385, 1006
BaeGI GKGCMC 2 cut(s) 94, 457
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 810
BbrPI CACGTG 1 cut(s) 1088
Bbv12I GWGCWC 2 cut(s) 94, 810
BbvI GCAGC 3 cut(s) 376, 1114, 1266
BccI CCATC 1 cut(s) 1211
BceAI ACGGC 1 cut(s) 954
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BciT130I CCWGG 3 cut(s) 395, 418, 564
BcoDI GTCTC 3 cut(s) 456, 863, 884
BcuI ACTAGT 1 cut(s) 503
BfaI CTAG 3 cut(s) 504, 654, 1218
BfuAI ACCTGC 1 cut(s) 62
BglII AGATCT 1 cut(s) 672
BisI GCNGC 3 cut(s) 365, 1103, 1280
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 3 cut(s) 366, 1104, 1281
Bme1390I CCNGG 3 cut(s) 395, 418, 564
Bme18I GGWCC 2 cut(s) 385, 1006
BmgBI CACGTC 1 cut(s) 569
BmgT120I GGNCC 2 cut(s) 385, 1006
BmiI GGNNCC 2 cut(s) 411, 1051
BmrFI CCNGG 3 cut(s) 395, 418, 564
BmrI ACTGGG 1 cut(s) 467
BmsI GCATC 3 cut(s) 91, 184, 951
BmuI ACTGGG 1 cut(s) 467
BplI GAGNNNNNCTC 6 cut(s) 122, 154, 453, 485, 580, 612
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 3 cut(s) 770, 836, 887
BsaAI YACGTR 1 cut(s) 1088
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 3 cut(s) 198, 417, 994
BsaWI WCCGGW 1 cut(s) 82
BsaXI ACNNNNNCTCC 2 cut(s) 721, 751
Bsc4I CCNNNNNNNGG 2 cut(s) 394, 400
Bse1I ACTGG 3 cut(s) 388, 462, 541
BseAI TCCGGA 1 cut(s) 82
BseBI CCWGG 3 cut(s) 395, 418, 564
BseDI CCNNGG 3 cut(s) 198, 417, 994
BseGI GGATG 3 cut(s) 307, 637, 1228
BseLI CCNNNNNNNGG 2 cut(s) 394, 400
BseMII CTCAG 3 cut(s) 332, 443, 920
BseNI ACTGG 3 cut(s) 388, 462, 541
BseRI GAGGAG 1 cut(s) 1289
BseSI GKGCMC 2 cut(s) 94, 457
BseXI GCAGC 3 cut(s) 376, 1114, 1266
BsgI GTGCAG 2 cut(s) 624, 1121
BshFI GGCC 2 cut(s) 150, 1295
BshNI GGYRCC 1 cut(s) 409
BsiHKAI GWGCWC 2 cut(s) 94, 810
BsiSI CCGG 1 cut(s) 83
BslFI GGGAC 1 cut(s) 514
BslI CCNNNNNNNGG 2 cut(s) 394, 400
BsmAI GTCTC 3 cut(s) 456, 863, 884
BsmFI GGGAC 1 cut(s) 514
BsmI GAATGC 1 cut(s) 1239
BsnI GGCC 2 cut(s) 150, 1295
Bso31I GGTCTC 1 cut(s) 884
Bsp1286I GDGCHC 3 cut(s) 94, 457, 810
Bsp13I TCCGGA 1 cut(s) 82
Bsp143I GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
Bsp1720I GCTNAGC 1 cut(s) 429
Bsp19I CCATGG 2 cut(s) 198, 994
BspACI CCGC 2 cut(s) 451, 586
BspANI GGCC 2 cut(s) 150, 1295
BspCNI CTCAG 3 cut(s) 331, 442, 919
BspEI TCCGGA 1 cut(s) 82
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 411, 1051
BspMI ACCTGC 1 cut(s) 62
BspPI GGATC 2 cut(s) 525, 675
BspT107I GGYRCC 1 cut(s) 409
BspTNI GGTCTC 1 cut(s) 884
BsrI ACTGG 3 cut(s) 388, 462, 541
BssECI CCNNGG 3 cut(s) 198, 417, 994
BssMI GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
BssT1I CCWWGG 2 cut(s) 198, 994
Bst2UI CCWGG 3 cut(s) 395, 418, 564
Bst4CI ACNGT 4 cut(s) 96, 111, 188, 982
Bst6I CTCTTC 1 cut(s) 603
BstAPI GCANNNNNTGC 1 cut(s) 77
BstBAI YACGTR 1 cut(s) 1088
BstC8I GCNNGC 2 cut(s) 453, 490
BstDEI CTNAG 4 cut(s) 120, 318, 429, 906
BstDSI CCRYGG 2 cut(s) 198, 994
BstF5I GGATG 3 cut(s) 307, 637, 1228
BstHHI GCGC 1 cut(s) 936
BstKTI GATC 8 cut(s) 520, 670, 675, 756, 883, 1046, 1078, 1300
BstMAI GTCTC 3 cut(s) 456, 863, 884
BstMBI GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
BstMWI GCNNNNNNNGC 3 cut(s) 77, 805, 1111
BstNI CCWGG 3 cut(s) 395, 418, 564
BstNSI RCATGY 2 cut(s) 548, 583
BstSCI CCNGG 3 cut(s) 393, 416, 562
BstSLI GKGCMC 2 cut(s) 94, 457
BstV1I GCAGC 3 cut(s) 376, 1114, 1266
BstX2I RGATCY 1 cut(s) 672
BstYI RGATCY 1 cut(s) 672
BsuRI GGCC 2 cut(s) 150, 1295
BtgI CCRYGG 2 cut(s) 198, 994
BtrI CACGTC 1 cut(s) 569
BtsCI GGATG 3 cut(s) 307, 637, 1228
BtsIMutI CAGTG 3 cut(s) 395, 455, 978
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 2 cut(s) 453, 490
CaiI CAGNNNCTG 1 cut(s) 694
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 936
Cfr13I GGNCC 2 cut(s) 385, 1006
CviAII CATG 8 cut(s) 182, 199, 485, 545, 580, 733, 995, 1030
DdeI CTNAG 4 cut(s) 120, 318, 429, 906
DpnI GATC 8 cut(s) 519, 669, 674, 755, 882, 1045, 1077, 1299
DpnII GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
DraIII CACNNNGTG 1 cut(s) 982
EaeI YGGCCR 1 cut(s) 1293
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 808
Eco130I CCWWGG 2 cut(s) 198, 994
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 884
Eco47I GGWCC 2 cut(s) 385, 1006
Eco53kI GAGCTC 1 cut(s) 808
Eco57I CTGAAG 3 cut(s) 165, 515, 762
Eco72I CACGTG 1 cut(s) 1088
EcoICRI GAGCTC 1 cut(s) 808
EcoRI GAATTC 2 cut(s) 525, 1037
EcoRII CCWGG 3 cut(s) 393, 416, 562
EcoT14I CCWWGG 2 cut(s) 198, 994
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 2 cut(s) 198, 994
FaeI CATG 8 cut(s) 185, 202, 488, 548, 583, 736, 998, 1033
FalI AAGNNNNNCTT 4 cut(s) 201, 233, 1098, 1130
FaqI GGGAC 1 cut(s) 514
FatI CATG 8 cut(s) 181, 198, 484, 544, 579, 732, 994, 1029
FauI CCCGC 1 cut(s) 444
Fnu4HI GCNGC 3 cut(s) 365, 1103, 1280
FokI GGATG 3 cut(s) 314, 644, 1235
FriOI GRGCYC 1 cut(s) 810
Fsp4HI GCNGC 3 cut(s) 365, 1103, 1280
FspBI CTAG 3 cut(s) 504, 654, 1218
GlaI GCGC 1 cut(s) 935
GluI GCNGC 3 cut(s) 365, 1103, 1280
HaeIII GGCC 2 cut(s) 150, 1295
HapII CCGG 1 cut(s) 83
HhaI GCGC 1 cut(s) 936
Hin1II CATG 8 cut(s) 185, 202, 488, 548, 583, 736, 998, 1033
Hin6I GCGC 1 cut(s) 934
HinP1I GCGC 1 cut(s) 934
HindIII AAGCTT 2 cut(s) 43, 863
HinfI GANTC 6 cut(s) 116, 133, 262, 307, 822, 1138
HpaII CCGG 1 cut(s) 83
HphI GGTGA 4 cut(s) 748, 802, 1010, 1279
Hpy166II GTNNAC 1 cut(s) 92
Hpy188I TCNGA 7 cut(s) 64, 145, 534, 672, 909, 1043, 1251
Hpy188III TCNNGA 7 cut(s) 83, 182, 324, 354, 512, 740, 1213
Hpy8I GTNNAC 1 cut(s) 92
HpyAV CCTTC 8 cut(s) 80, 140, 264, 436, 472, 708, 1166, 1293
HpyCH4III ACNGT 4 cut(s) 96, 111, 188, 982
HpyCH4IV ACGT 3 cut(s) 568, 1087, 1290
HpyCH4V TGCA 6 cut(s) 92, 364, 544, 605, 899, 1102
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 805, 1111
HpyF3I CTNAG 4 cut(s) 120, 318, 429, 906
HpySE526I ACGT 3 cut(s) 568, 1087, 1290
Hsp92II CATG 8 cut(s) 185, 202, 488, 548, 583, 736, 998, 1033
HspAI GCGC 1 cut(s) 934
Kpn2I TCCGGA 1 cut(s) 82
Kzo9I GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
LmnI GCTCC 2 cut(s) 85, 1276
Lsp1109I GCAGC 3 cut(s) 376, 1114, 1266
LweI GCATC 3 cut(s) 91, 184, 951
MaeI CTAG 3 cut(s) 504, 654, 1218
MaeII ACGT 3 cut(s) 568, 1087, 1290
MaeIII GTNAC 3 cut(s) 971, 1019, 1213
MalI GATC 8 cut(s) 519, 669, 674, 755, 882, 1045, 1077, 1299
MboI GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
MboII GAAGA 6 cut(s) 151, 521, 590, 866, 1200, 1292
MflI RGATCY 1 cut(s) 672
MhlI GDGCHC 3 cut(s) 94, 457, 810
MluCI AATT 9 cut(s) 5, 276, 357, 525, 830, 1037, 1119, 1123, 1207
MlyI GAGTC 1 cut(s) 1132
MnlI CCTC 9 cut(s) 169, 266, 508, 582, 747, 811, 1063, 1245, 1267
MroI TCCGGA 1 cut(s) 82
MseI TTAA 6 cut(s) 221, 615, 795, 840, 924, 1122
MslI CAYNNNNRTG 2 cut(s) 617, 1028
MspI CCGG 1 cut(s) 83
MspR9I CCNGG 3 cut(s) 395, 418, 564
Mva1269I GAATGC 1 cut(s) 1239
MvaI CCWGG 3 cut(s) 395, 418, 564
MwoI GCNNNNNNNGC 3 cut(s) 77, 805, 1111
NcoI CCATGG 2 cut(s) 198, 994
NdeII GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
NlaIII CATG 8 cut(s) 185, 202, 488, 548, 583, 736, 998, 1033
NlaIV GGNNCC 2 cut(s) 411, 1051
NmeAIII GCCGAG 1 cut(s) 450
NmuCI GTSAC 3 cut(s) 971, 1019, 1213
NspI RCATGY 2 cut(s) 548, 583
PagI TCATGA 1 cut(s) 181
PciI ACATGT 1 cut(s) 579
PcsI WCGNNNNNNNCGW 2 cut(s) 1168, 1177
PctI GAATGC 1 cut(s) 1239
PfeI GAWTC 5 cut(s) 116, 133, 262, 307, 822
PflMI CCANNNNNTGG 1 cut(s) 400
PfoI TCCNGGA 1 cut(s) 562
PkrI GCNGC 3 cut(s) 366, 1104, 1281
PleI GAGTC 1 cut(s) 1132
PmaCI CACGTG 1 cut(s) 1088
PmlI CACGTG 1 cut(s) 1088
PpsI GAGTC 1 cut(s) 1132
Ppu21I YACGTR 1 cut(s) 1088
PscI ACATGT 1 cut(s) 579
PshBI ATTAAT 1 cut(s) 1122
PsiI TTATAA 1 cut(s) 1260
Psp124BI GAGCTC 1 cut(s) 810
Psp6I CCWGG 3 cut(s) 393, 416, 562
PspCI CACGTG 1 cut(s) 1088
PspGI CCWGG 3 cut(s) 393, 416, 562
PspN4I GGNNCC 2 cut(s) 411, 1051
PspPI GGNCC 2 cut(s) 385, 1006
PstNI CAGNNNCTG 1 cut(s) 694
PsuI RGATCY 1 cut(s) 672
RseI CAYNNNNRTG 2 cut(s) 617, 1028
SacI GAGCTC 1 cut(s) 810
SaqAI TTAA 6 cut(s) 221, 615, 795, 840, 924, 1122
SatI GCNGC 3 cut(s) 365, 1103, 1280
Sau3AI GATC 8 cut(s) 517, 667, 672, 753, 880, 1043, 1075, 1297
Sau96I GGNCC 2 cut(s) 385, 1006
SchI GAGTC 1 cut(s) 1132
ScrFI CCNGG 3 cut(s) 395, 418, 564
SduI GDGCHC 3 cut(s) 94, 457, 810
SfaNI GCATC 3 cut(s) 91, 184, 951
SinI GGWCC 2 cut(s) 385, 1006
SmiMI CAYNNNNRTG 2 cut(s) 617, 1028
SmlI CTYRAG 3 cut(s) 749, 815, 866
SmoI CTYRAG 3 cut(s) 749, 815, 866
SpeI ACTAGT 1 cut(s) 503
Sse9I AATT 9 cut(s) 5, 276, 357, 525, 830, 1037, 1119, 1123, 1207
SsiI CCGC 2 cut(s) 451, 586
SspMI CTAG 3 cut(s) 504, 654, 1218
SstI GAGCTC 1 cut(s) 810
StyD4I CCNGG 3 cut(s) 393, 416, 562
StyI CCWWGG 2 cut(s) 198, 994
TaaI ACNGT 4 cut(s) 96, 111, 188, 982
TaiI ACGT 3 cut(s) 571, 1090, 1293
TaqI TCGA 1 cut(s) 846
TasI AATT 9 cut(s) 5, 276, 357, 525, 830, 1037, 1119, 1123, 1207
TfiI GAWTC 5 cut(s) 116, 133, 262, 307, 822
Tru1I TTAA 6 cut(s) 221, 615, 795, 840, 924, 1122
Tru9I TTAA 6 cut(s) 221, 615, 795, 840, 924, 1122
TscAI CASTG 3 cut(s) 395, 462, 985
TseFI GTSAC 3 cut(s) 971, 1019, 1213
TseI GCWGC 3 cut(s) 364, 1102, 1279
Tsp45I GTSAC 3 cut(s) 971, 1019, 1213
TspDTI ATGAA 9 cut(s) 17, 39, 140, 170, 473, 648, 657, 785, 1148
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 3 cut(s) 395, 462, 985
Van91I CCANNNNNTGG 1 cut(s) 400
VneI GTGCAC 1 cut(s) 90
VpaK11BI GGWCC 2 cut(s) 385, 1006
VspI ATTAAT 1 cut(s) 1122
XapI RAATTY 4 cut(s) 5, 357, 525, 1037
XceI RCATGY 2 cut(s) 548, 583
XspI CTAG 3 cut(s) 504, 654, 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.