Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001673

Physical Location & Seq

Genomic Coordinates Forward (+)

252833 .. 255377

Gene Structure 2545 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g00470.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGGCTTATCATGTATCCTCCACACCCTCCTCTCTCTTTCTCTCTCACTCTCTCTCCTCCTCTAGAAGGCTCAGGCCAACCACCTCTGATACTCACACCTTCCCTACTCTTCACTCCAGAATTTCTCTCCAAATAAACCATGTTTCTTTACAAGAAACCCCAAAAACTGAAAACCCCGATGGAAATTCCGAGTCTTCCTCCAAAAGCTATGTTTGGGTCAATCCCAGAAGCCCCAGAGCTTCTCATCTTAGGAACAAGTCCTACGATTCCAGGTACACTTCTTTAGTCAAAGTAGCTGAGTCCTTGAACTCTGTCGGCCTAAGTGAAAGTGATGTTGTTGATATTTTAAAGACTTTGGGTGATGGGGTATTGGAACAAGATGCTGTCGTTGTTCTTAATACCATGGTTAACCCCGAAAATGCATTACTTGCTCTAAAGTACTTTCAGCAGAGGTTTAAACCTAAGAGGGAAGTGGTTCTCTACAATGTGACATTGAAAGTGTTTAGAAAGTGTAAGGATTTAGTGAGAGCAGAGAAGCTGTTTAATGAGATGATTGAGAGAGGTGTTACACCTGATAATGTTACGTTTTCGACTATGATTAGCTGTGCTAGGATGTGTTATCTGCCTGATAAGGCTGTGGAGTGGTTTGAGAAGATGCCTAGTTACGGGTGTAGTCCTGATGATGTTACCTATTCGGCTATGATTGATGCATATGGCCGTGCCGGTAATGTTGACAAGGCTTTTAGCTTGTATGACCGCGCTAGGACAGAGAAATGGCGCATTGATCCAGTGACATTTGCAACGTTGATTAAGATTCACGGGCAATCAGGGAATTATGATGGGTGCTTGAATGTTTATGAGGAAATGAAGGCAATTGGGGTTAAGCCGAACATGGTTATTTATAATACTTTGTTGGATGCTATGGGAAGAGCTAAGAGGCCTTGGCAGGCCAAGAAAATTTACCGAGAGATGATCAGCAAGGGGTCTTCGCCAAATTGGGTGACCTATGCATCTCTGTTAAGGGCCTTTGGTAGAGCTCGCTATGGTGATGATGCTCTGAATGTGTACAAGGAGATGAAGGAGAAGGGAATGGAGTTGAATGTAATTCTCTATAACACCCTGTTGTCTATGTGTGCTGATATTGGCTACACTGATCAAGCTATTGAGATTTTCAAAGACATGAAGAGTTCCGAAACTTTGAAGCCTGATAGCTGGACGTTTTCGTCCATGATTACCATATATTCCTGTAGCGGGAAAGTCTCAGAGGCAGAGAGCATGTTGAATGAGATGGTGGAAGCTGGTTTCCAGCCTAATATCTTTATTTTGACATCACTAATACAATGTTATGGCAAGGCCAAGCGTACTGATGATGTTGTCAGGGCATTCAATCAACTACTAGAATTGGGCATAACTCCTGATGAGCGATTCTGTGGCTGTCTCCTGAATGTGATGACCCTAACACCAAAAGATGAACTATCTAAGCTGACTGATTGCATCGAGCGGGGTAATGAGAAGCTTGGGTCTGTGGTAAGACTTCTTGTAGAGACGCAAGACAGTAGTGAAAACTTCAAGAAGGAAGCATCAGAACTTTTAACTTCAGTTGGTTCCGATGTAAAGAAGGCATACTGTAATTGCTTGATCGATCTTTGTGTGAATCTTGATCTCTTGGAGAGAGCCTGTGAGCTTCTAGACCTAGGGCTTACACTGGAGATATACACAGATATACAGTCAAGGTCTCCAACCCAGTGGTCTCTGTATCTCAAGGGTCTCTCTCTTGGAGCAGCTCTTACTGCATTACATGTTTGGATTAATGACTTGTCTCGTTCATTGGAATCTGGGGAGGAGCTTCCACCTCTGCTGGGAATTAATACCGGACATGGCAAACATAAATATTCAGACAAAGGTCTAGCTAGTGTGTTCGAGTCACATTTGAAGGAAGTAGATGCTCCGTTCCATGAGGCCCCAGACAAGGTTGGCTGGTTTTTGACTACAAAGGTTGCAGTCACGTCATGGTTGGAGTCTAGAAGGTCACCAGAAGTAGTTGCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016070 GO:0016787 GO:0016788 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031425 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

684

Amino Acids

76.6

Weight (kDa)

6.25

Isoelectric Point (pI)

35.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	157 - 202	2.4e-11	PPR repeat family
PPR_long	PF17177	167 - 267	8.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	209 - 238	2.8e-07	PPR repeat family
TPR_24	PF23276	211 - 324	1.3e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	223 - 253	5.2e-10	PPR repeat
PPR_2	PF13041	229 - 271	7.6e-07	PPR repeat family
PPR	PF01535	229 - 253	5.3e-07	PPR repeat
PPR_3	PF13812	260 - 309	1.1e-07	Pentatricopeptide repeat domain
TPR_24	PF23276	275 - 394	2.3e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	296 - 344	1e-11	PPR repeat family
PPR_3	PF13812	319 - 379	2e-11	Pentatricopeptide repeat domain
PPR	PF01535	334 - 364	7.4e-06	PPR repeat
PPR_long	PF17177	351 - 485	6.1e-14	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	356 - 414	1.7e-09	Pentatricopeptide repeat domain
PPR_2	PF13041	367 - 412	1.5e-09	PPR repeat family
PPR_2	PF13041	402 - 440	7.5e-06	PPR repeat family
PPR	PF01535	406 - 435	7.8e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001673)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16390
fragaria_vesca	FvH4_3g00440 FvH4_3g00470
malus_domestica	MD00G1221000.v1.1 MD05G1360400.v1.1 MD08G1234300.v1.1 MD10G1337400.v1.1 MD10G1337800.v1.1 MD10G1338000.v1.1
prunus_persica	Prupe.3G138500_v2.0.a1 Prupe.4G005200_v2.0.a1
pyrus_communis	pycom05g32720 pycom10g28680 pycom10g28700 pycom10g28720 pycom10g28750
rosa_chinensis	RchiOBHm_Chr1g0364151 RchiOBHm_Chr2g0155431 RchiOBHm_Chr4g0402181 RchiOBHm_Chr5g0000631 RchiOBHm_Chr6g0271321
rosa_laevigata	RLG00000004254 RLG00000013858 RLG00000028931 RLG00000030883
rosa_multiflora	Rmu_co8004710.1_g000001 Rmu_co8021096.1_g000001 Rmu_co8161794.1_g000001 Rmu_sc0000795.1_g000110 Rmu_sc0001141.1_g000016 Rmu_sc0003662.1_g000007 Rmu_sc0003699.1_g000009 Rmu_sc0006695.1_g000081 Rmu_sc0010255.1_g000007 Rmu_sc0011992.1_g000014 Rmu_sc0016929.1_g000002 Rmu_sc0017523.1_g000008 Rmu_sc0021532.1_g000001 Rmu_sc0029877.1_g000001
rosa_roxburghii	Rroxscaffold_1G00001310 Rroxscaffold_1G00075570 Rroxscaffold_4G00324290
rosa_rugosa	Rorug04G0385500 Rorug07G0309300.1
rosa_samantha	Rh5AG005400 Rh5BG007000 Rh5CG005700 Rh5DG005600
rosa_wichuraiana	Rw5G000530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	903
AasI	GACNNNNNNGTC	1 cut(s)	1222
AccBSI	CCGCTC	1 cut(s)	1501
AccII	CGCG	1 cut(s)	759
AciI	CCGC	3 cut(s)	757, 1251, 1501
AclI	AACGTT	1 cut(s)	803
AclWI	GGATC	1 cut(s)	779
AcoI	YGGCCR	1 cut(s)	715
AcsI	RAATTY	3 cut(s)	120, 184, 957
AcuI	CTGAAG	1 cut(s)	1581
AfaI	GTAC	4 cut(s)	275, 440, 1067, 1363
AfiI	CCNNNNNNNGG	5 cut(s)	66, 665, 1251, 1859, 1969
AflIII	ACRYGT	1 cut(s)	1798
AjiI	CACGTC	1 cut(s)	2007
AjnI	CCWGG	1 cut(s)	269
Alw21I	GWGCWC	1 cut(s)	1039
Alw26I	GTCTC	7 cut(s)	1264, 1442, 1538, 1740, 1755, 1772, 1824
AlwI	GGATC	1 cut(s)	779
AoxI	GGCC	8 cut(s)	74, 316, 715, 938, 948, 1023, 1353, 1959
ApeKI	GCWGC	2 cut(s)	1781, 2045
ApoI	RAATTY	3 cut(s)	120, 184, 957
AseI	ATTAAT	2 cut(s)	1809, 1866
Asp700I	GAANNNNTTC	1 cut(s)	474
AspA2I	CCTAGG	1 cut(s)	1693
AspLEI	GCGC	2 cut(s)	761, 780
AspS9I	GGNCC	2 cut(s)	1023, 1960
AsuHPI	GGTGA	4 cut(s)	371, 1012, 1058, 2022
AvrII	CCTAGG	1 cut(s)	1693
BanII	GRGCYC	1 cut(s)	1039
BbsI	GAAGAC	2 cut(s)	186, 978
Bbv12I	GWGCWC	1 cut(s)	1039
BbvI	GCAGC	2 cut(s)	1793, 2032
BccI	CCATC	4 cut(s)	173, 356, 833, 1282
BceAI	ACGGC	1 cut(s)	702
BciT130I	CCWGG	1 cut(s)	271
BciVI	GTATCC	1 cut(s)	25
BclI	TGATCA	2 cut(s)	972, 1153
BcoDI	GTCTC	7 cut(s)	1264, 1442, 1538, 1740, 1755, 1772, 1824
BfmI	CTRYAG	1 cut(s)	1246
BfuI	GTATCC	1 cut(s)	25
BisI	GCNGC	2 cut(s)	1782, 2046
BlnI	CCTAGG	1 cut(s)	1693
BlsI	GCNGC	2 cut(s)	1783, 2047
BmcAI	AGTACT	1 cut(s)	440
Bme1390I	CCNGG	1 cut(s)	271
BmgBI	CACGTC	1 cut(s)	2007
BmgT120I	GGNCC	2 cut(s)	1023, 1960
BmiI	GGNNCC	2 cut(s)	1606, 1962
BmrFI	CCNGG	1 cut(s)	271
BmrI	ACTGGG	1 cut(s)	1738
BmsI	GCATC	9 cut(s)	370, 645, 697, 907, 1019, 1042, 1503, 1589, 1933
BmuI	ACTGGG	1 cut(s)	1738
BoxI	GACNNNNGTC	1 cut(s)	1902
BpiI	GAAGAC	2 cut(s)	186, 978
BplI	GAGNNNNNCTC	2 cut(s)	182, 214
BpmI	CTGGAG	2 cut(s)	100, 1727
Bpu10I	CCTNAGC	1 cut(s)	71
BpuEI	CTTGAG	1 cut(s)	1745
Bsa29I	ATCGAT	1 cut(s)	1641
BsaI	GGTCTC	3 cut(s)	1740, 1755, 1772
BsaJI	CCNNGG	3 cut(s)	402, 941, 1693
BsaWI	WCCGGW	1 cut(s)	1871
BsaXI	ACNNNNNCTCC	2 cut(s)	38, 68
Bsc4I	CCNNNNNNNGG	5 cut(s)	66, 665, 1251, 1859, 1969
Bse118I	RCCGGY	1 cut(s)	722
Bse1I	ACTGG	3 cut(s)	788, 1710, 1744
BseBI	CCWGG	1 cut(s)	271
BseCI	ATCGAT	1 cut(s)	1641
BseDI	CCNNGG	3 cut(s)	402, 941, 1693
BseGI	GGATG	2 cut(s)	618, 922
BseLI	CCNNNNNNNGG	5 cut(s)	66, 665, 1251, 1859, 1969
BseMII	CTCAG	3 cut(s)	85, 288, 1275
BseNI	ACTGG	3 cut(s)	788, 1710, 1744
BseRI	GAGGAG	4 cut(s)	19, 46, 49, 1856
BseXI	GCAGC	2 cut(s)	1793, 2032
BseYI	CCCAGC	1 cut(s)	1858
Bsh1236I	CGCG	1 cut(s)	759
BshFI	GGCC	8 cut(s)	76, 318, 717, 940, 950, 1025, 1355, 1961
BshVI	ATCGAT	1 cut(s)	1641
BsiHKAI	GWGCWC	1 cut(s)	1039
BsiSI	CCGG	2 cut(s)	723, 1872
BslI	CCNNNNNNNGG	5 cut(s)	66, 665, 1251, 1859, 1969
BsmAI	GTCTC	7 cut(s)	1264, 1442, 1538, 1740, 1755, 1772, 1824
BsmBI	CGTCTC	1 cut(s)	1538
BsmI	GAATGC	1 cut(s)	1382
BsnI	GGCC	8 cut(s)	76, 318, 717, 940, 950, 1025, 1355, 1961
Bso31I	GGTCTC	3 cut(s)	1740, 1755, 1772
Bsp1286I	GDGCHC	1 cut(s)	1039
Bsp1407I	TGTACA	1 cut(s)	1065
Bsp143I	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
Bsp19I	CCATGG	1 cut(s)	402
BspACI	CCGC	3 cut(s)	757, 1251, 1501
BspANI	GGCC	8 cut(s)	76, 318, 717, 940, 950, 1025, 1355, 1961
BspCNI	CTCAG	3 cut(s)	84, 289, 1274
BspDI	ATCGAT	1 cut(s)	1641
BspFNI	CGCG	1 cut(s)	759
BspLI	GGNNCC	2 cut(s)	1606, 1962
BspPI	GGATC	1 cut(s)	779
BspQI	GCTCTTC	1 cut(s)	922
BspTNI	GGTCTC	3 cut(s)	1740, 1755, 1772
BsrBI	CCGCTC	1 cut(s)	1501
BsrFI	RCCGGY	1 cut(s)	722
BsrGI	TGTACA	1 cut(s)	1065
BsrI	ACTGG	3 cut(s)	788, 1710, 1744
BssAI	RCCGGY	1 cut(s)	722
BssECI	CCNNGG	3 cut(s)	402, 941, 1693
BssMI	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
BssT1I	CCWWGG	3 cut(s)	402, 941, 1693
Bst2UI	CCWGG	1 cut(s)	271
Bst4CI	ACNGT	3 cut(s)	1556, 1628, 1728
Bst6I	CTCTTC	3 cut(s)	114, 922, 1178
BstAPI	GCANNNNNTGC	1 cut(s)	428
BstAUI	TGTACA	1 cut(s)	1065
BstC8I	GCNNGC	2 cut(s)	948, 1039
BstDEI	CTNAG	9 cut(s)	71, 248, 297, 320, 462, 933, 1261, 1479, 2049
BstDSI	CCRYGG	1 cut(s)	402
BstEII	GGTNACC	2 cut(s)	1000, 2028
BstENI	CCTNNNNNAGG	1 cut(s)	64
BstF5I	GGATG	2 cut(s)	618, 922
BstFNI	CGCG	1 cut(s)	759
BstHHI	GCGC	2 cut(s)	761, 780
BstKTI	GATC	6 cut(s)	787, 975, 1156, 1641, 1645, 1663
BstMAI	GTCTC	7 cut(s)	1264, 1442, 1538, 1740, 1755, 1772, 1824
BstMBI	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
BstMWI	GCNNNNNNNGC	2 cut(s)	428, 1790
BstNI	CCWGG	1 cut(s)	271
BstNSI	RCATGY	2 cut(s)	1279, 1802
BstPAI	GACNNNNGTC	1 cut(s)	1902
BstPI	GGTNACC	2 cut(s)	1000, 2028
BstSCI	CCNGG	1 cut(s)	269
BstSFI	CTRYAG	1 cut(s)	1246
BstUI	CGCG	1 cut(s)	759
BstV1I	GCAGC	2 cut(s)	1793, 2032
BstV2I	GAAGAC	2 cut(s)	186, 978
BstXI	CCANNNNNNTGG	1 cut(s)	1746
Bsu15I	ATCGAT	1 cut(s)	1641
BsuI	GTATCC	1 cut(s)	25
BsuRI	GGCC	8 cut(s)	76, 318, 717, 940, 950, 1025, 1355, 1961
BsuTUI	ATCGAT	1 cut(s)	1641
BtgI	CCRYGG	1 cut(s)	402
BtrI	CACGTC	1 cut(s)	2007
BtsCI	GGATG	2 cut(s)	618, 922
BtsIMutI	CAGTG	4 cut(s)	795, 1149, 1703, 1751
Cac8I	GCNNGC	2 cut(s)	948, 1039
CfoI	GCGC	2 cut(s)	761, 780
Cfr10I	RCCGGY	1 cut(s)	722
Cfr13I	GGNCC	2 cut(s)	1023, 1960
ClaI	ATCGAT	1 cut(s)	1641
CseI	GACGC	1 cut(s)	1555
Csp6I	GTAC	4 cut(s)	274, 439, 1066, 1362
CviQI	GTAC	4 cut(s)	274, 439, 1066, 1362
DdeI	CTNAG	9 cut(s)	71, 248, 297, 320, 462, 933, 1261, 1479, 2049
DpnI	GATC	6 cut(s)	786, 974, 1155, 1640, 1644, 1662
DpnII	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
DraI	TTTAAA	2 cut(s)	348, 457
DrdI	GACNNNNNNGTC	1 cut(s)	1222
DseDI	GACNNNNNNGTC	1 cut(s)	1222
EaeI	YGGCCR	1 cut(s)	715
Eam1104I	CTCTTC	3 cut(s)	114, 922, 1178
EarI	CTCTTC	3 cut(s)	114, 922, 1178
Ecl136II	GAGCTC	1 cut(s)	1037
Eco130I	CCWWGG	3 cut(s)	402, 941, 1693
Eco147I	AGGCCT	1 cut(s)	940
Eco24I	GRGCYC	1 cut(s)	1039
Eco31I	GGTCTC	3 cut(s)	1740, 1755, 1772
Eco53kI	GAGCTC	1 cut(s)	1037
Eco57I	CTGAAG	1 cut(s)	1581
Eco91I	GGTNACC	2 cut(s)	1000, 2028
EcoICRI	GAGCTC	1 cut(s)	1037
EcoNI	CCTNNNNNAGG	1 cut(s)	64
EcoO109I	RGGNCCY	2 cut(s)	1023, 1960
EcoO65I	GGTNACC	2 cut(s)	1000, 2028
EcoRII	CCWGG	1 cut(s)	269
EcoT14I	CCWWGG	3 cut(s)	402, 941, 1693
EcoT22I	ATGCAT	3 cut(s)	424, 712, 1012
EcoT38I	GRGCYC	1 cut(s)	1039
ErhI	CCWWGG	3 cut(s)	402, 941, 1693
Esp3I	CGTCTC	1 cut(s)	1538
FauI	CCCGC	2 cut(s)	1244, 1494
FauNDI	CATATG	1 cut(s)	712
FbaI	TGATCA	2 cut(s)	972, 1153
Fnu4HI	GCNGC	2 cut(s)	1782, 2046
FokI	GGATG	2 cut(s)	625, 929
FriOI	GRGCYC	1 cut(s)	1039
Fsp4HI	GCNGC	2 cut(s)	1782, 2046
GlaI	GCGC	2 cut(s)	760, 779
GluI	GCNGC	2 cut(s)	1782, 2046
GsaI	CCCAGC	1 cut(s)	1862
GsuI	CTGGAG	2 cut(s)	100, 1727
HaeIII	GGCC	8 cut(s)	76, 318, 717, 940, 950, 1025, 1355, 1961
HapII	CCGG	2 cut(s)	723, 1872
HgaI	GACGC	1 cut(s)	1555
HhaI	GCGC	2 cut(s)	761, 780
Hin6I	GCGC	2 cut(s)	759, 778
HinP1I	GCGC	2 cut(s)	759, 778
HincII	GTYRAC	2 cut(s)	409, 733
HindII	GTYRAC	2 cut(s)	409, 733
HindIII	AAGCTT	1 cut(s)	1514
HinfI	GANTC	9 cut(s)	191, 266, 299, 814, 1425, 1654, 1832, 1922, 2018
HpaI	GTTAAC	1 cut(s)	409
HpaII	CCGG	2 cut(s)	723, 1872
HphI	GGTGA	4 cut(s)	371, 1012, 1058, 2022
Hpy166II	GTNNAC	4 cut(s)	276, 409, 733, 1066
Hpy188I	TCNGA	8 cut(s)	88, 190, 1059, 1192, 1264, 1585, 1609, 1897
Hpy188III	TCNNGA	9 cut(s)	63, 117, 677, 1415, 1441, 1570, 1658, 1688, 2022
Hpy8I	GTNNAC	4 cut(s)	276, 409, 733, 1066
HpyAV	CCTTC	9 cut(s)	60, 109, 862, 1072, 1078, 1567, 1612, 1927, 2019
HpyCH4III	ACNGT	3 cut(s)	1556, 1628, 1728
HpyCH4IV	ACGT	4 cut(s)	584, 803, 1217, 2006
HpyCH4V	TGCA	7 cut(s)	422, 710, 800, 1010, 1494, 1793, 2000
HpyF10VI	GCNNNNNNNGC	2 cut(s)	428, 1790
HpyF3I	CTNAG	9 cut(s)	71, 248, 297, 320, 462, 933, 1261, 1479, 2049
HpySE526I	ACGT	4 cut(s)	584, 803, 1217, 2006
HspAI	GCGC	2 cut(s)	759, 778
Ksp22I	TGATCA	2 cut(s)	972, 1153
KspAI	GTTAAC	1 cut(s)	409
Kzo9I	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
LguI	GCTCTTC	1 cut(s)	922
LmnI	GCTCC	3 cut(s)	1778, 1843, 1951
Lsp1109I	GCAGC	2 cut(s)	1793, 2032
LweI	GCATC	9 cut(s)	370, 645, 697, 907, 1019, 1042, 1503, 1589, 1933
MaeII	ACGT	4 cut(s)	584, 803, 1217, 2006
MalI	GATC	6 cut(s)	786, 974, 1155, 1640, 1644, 1662
MbiI	CCGCTC	1 cut(s)	1501
MboI	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
MboII	GAAGA	6 cut(s)	101, 186, 664, 939, 978, 1195
MfeI	CAATTG	1 cut(s)	873
MhlI	GDGCHC	1 cut(s)	1039
MlyI	GAGTC	4 cut(s)	200, 308, 1931, 2027
MmeI	TCCRAC	3 cut(s)	894, 1763, 1995
Mph1103I	ATGCAT	3 cut(s)	424, 712, 1012
MroXI	GAANNNNTTC	1 cut(s)	474
MspI	CCGG	2 cut(s)	723, 1872
MspR9I	CCNGG	1 cut(s)	271
MssI	GTTTAAAC	1 cut(s)	457
MunI	CAATTG	1 cut(s)	873
Mva1269I	GAATGC	1 cut(s)	1382
MvaI	CCWGG	1 cut(s)	271
MvnI	CGCG	1 cut(s)	759
MwoI	GCNNNNNNNGC	2 cut(s)	428, 1790
NcoI	CCATGG	1 cut(s)	402
NdeI	CATATG	1 cut(s)	712
NdeII	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
NlaIV	GGNNCC	2 cut(s)	1606, 1962
NmuCI	GTSAC	6 cut(s)	487, 790, 1000, 1923, 2002, 2028
NsiI	ATGCAT	3 cut(s)	424, 712, 1012
NspI	RCATGY	2 cut(s)	1279, 1802
PceI	AGGCCT	1 cut(s)	940
PciI	ACATGT	1 cut(s)	1798
PciSI	GCTCTTC	1 cut(s)	922
PctI	GAATGC	1 cut(s)	1382
PdmI	GAANNNNTTC	1 cut(s)	474
PfeI	GAWTC	5 cut(s)	266, 814, 1425, 1654, 1832
PkrI	GCNGC	2 cut(s)	1783, 2047
PleI	GAGTC	4 cut(s)	199, 307, 1930, 2026
PmeI	GTTTAAAC	1 cut(s)	457
PpsI	GAGTC	4 cut(s)	199, 307, 1930, 2026
PscI	ACATGT	1 cut(s)	1798
PshAI	GACNNNNGTC	1 cut(s)	1902
PshBI	ATTAAT	2 cut(s)	1809, 1866
PsiI	TTATAA	1 cut(s)	903
Psp124BI	GAGCTC	1 cut(s)	1039
Psp1406I	AACGTT	1 cut(s)	803
Psp6I	CCWGG	1 cut(s)	269
PspEI	GGTNACC	2 cut(s)	1000, 2028
PspFI	CCCAGC	1 cut(s)	1858
PspGI	CCWGG	1 cut(s)	269
PspN4I	GGNNCC	2 cut(s)	1606, 1962
PspPI	GGNCC	2 cut(s)	1023, 1960
PsrI	GAACNNNNNNTAC	2 cut(s)	245, 277
RsaI	GTAC	4 cut(s)	275, 440, 1067, 1363
RsaNI	GTAC	4 cut(s)	274, 439, 1066, 1362
SacI	GAGCTC	1 cut(s)	1039
SapI	GCTCTTC	1 cut(s)	922
SatI	GCNGC	2 cut(s)	1782, 2046
Sau3AI	GATC	6 cut(s)	784, 972, 1153, 1638, 1642, 1660
Sau96I	GGNCC	2 cut(s)	1023, 1960
ScaI	AGTACT	1 cut(s)	440
SchI	GAGTC	4 cut(s)	200, 308, 1931, 2027
ScrFI	CCNGG	1 cut(s)	271
SduI	GDGCHC	1 cut(s)	1039
SfaNI	GCATC	9 cut(s)	370, 645, 697, 907, 1019, 1042, 1503, 1589, 1933
SfcI	CTRYAG	1 cut(s)	1246
SmlI	CTYRAG	1 cut(s)	1760
SmoI	CTYRAG	1 cut(s)	1760
SseBI	AGGCCT	1 cut(s)	940
SsiI	CCGC	3 cut(s)	757, 1251, 1501
SspI	AATATT	1 cut(s)	1892
SstI	GAGCTC	1 cut(s)	1039
StuI	AGGCCT	1 cut(s)	940
StyD4I	CCNGG	1 cut(s)	269
StyI	CCWWGG	3 cut(s)	402, 941, 1693
TaaI	ACNGT	3 cut(s)	1556, 1628, 1728
TaiI	ACGT	4 cut(s)	587, 806, 1220, 2009
TaqI	TCGA	4 cut(s)	590, 1497, 1641, 1920
TatI	WGTACW	2 cut(s)	438, 1065
TfiI	GAWTC	5 cut(s)	266, 814, 1425, 1654, 1832
TscAI	CASTG	4 cut(s)	795, 1156, 1710, 1751
TseFI	GTSAC	6 cut(s)	487, 790, 1000, 1923, 2002, 2028
TseI	GCWGC	2 cut(s)	1781, 2045
Tsp45I	GTSAC	6 cut(s)	487, 790, 1000, 1923, 2002, 2028
TspDTI	ATGAA	5 cut(s)	881, 1091, 1196, 1485, 1815
TspGWI	ACGGA	1 cut(s)	1938
TspRI	CASTG	4 cut(s)	795, 1156, 1710, 1751
VspI	ATTAAT	2 cut(s)	1809, 1866
XagI	CCTNNNNNAGG	1 cut(s)	64
XapI	RAATTY	3 cut(s)	120, 184, 957
XbaI	TCTAGA	3 cut(s)	62, 1687, 2021
XceI	RCATGY	2 cut(s)	1279, 1802
XmaJI	CCTAGG	1 cut(s)	1693
XmnI	GAANNNNTTC	1 cut(s)	474
ZrmI	AGTACT	1 cut(s)	440
Zsp2I	ATGCAT	3 cut(s)	424, 712, 1012

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.