Rmu_sc0003662.1_g000007

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003662.1
OG0001673
Physical Location & Seq
Reverse (-)
21971 .. 23761
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003662.1_g000007.1.cds

Sequence Viewer

Length: 1491 bp
atggcataccatggctacgatcccggggatcgtaacaactggtatcagagcccgcaattgggcttgtttccggtgagtccggcggtgggctctccggcgcttgtaccacacacatgcgtctcatcctcacgtccccacctttttaccccatctccaatttccgaacccatttccatcattcagagtcggatctcccaccacaccaaccaccactaccgtagctttgacacttgccttcggctcaactatatcacagcctatgtgagctcaaccccatctcagtccttgtttggggcttcctcgtcaccgacgtcaatttccaatattccagagcagaatgaggtgctcaactctaagcccgtcgtccctgccatcatggacgagctcgagaagctacgccatcaggaacatggcattcagcctgatggccaaataccgagtgataagactatcgacggaggagacgatgcctttaacactttcttcagtgaaactggagctagaaaacacgttcctcgcgccgtgttcttggatctggagcccaccgtcatcgacaaggtgaggaacggaacttactgtcagctgttccaccccgagcagctcattagcggcaaagaggacgctgccaataacttcactagaggtcattacacaatttggaagaagattgtggatctgtgcttggatctgatccagaagcttgcggacaactgtactgggcttcaagggtttctggtgttccatgctgtcggaagtgggactggatctgggcttggatctctgctcttggagaggctatctgtggactatgggaagaaatctaagcttggattcactgtttacccatctcctcaggtctcaacctctgttgttgagccttacaatagtgttttgaccactcactcgctcttggagcacactgatgtggctgtgcttctggacaatgaagccatctatgatatctactgcagatcgcttgacattgagcgacctacctacaccaatctcaacaggcttgtttctcaggtcatttactcattcactgcttctctgcgttttgatccagcgtcaggtgttcaagcaccattagagtgtcatccattatcagttgttagaattgatgagtgcttgtcttcgcttgattttgttttgatttttgagaagaatgagaagttttcaagtcgtagtcctgccatgaattttgtaaattgcttggagactatagaagatgctaaaaaattgtttctgaaaatgcatgaaaggaacattgtttcatggaatactttgattagtgcattggtccgaattggtcaggaggaaaaggcaatggctgtttattgtgctatggtttcggagggagtggtgcctcctaattcaaatctctacatgaggcctcctgatgatttcaaggttgataatcacagatgtgatttccaaagaattttgattcaactgctgactgctttctacttgagatacacaaataagtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04145 ko04210 ko04530 ko04540 ko05130 map04145 map04210 map04530 map04540 map05130
Pfam Domains
Protein Families
Tubulin Tubulin_C

Protein Analysis

496

Amino Acids

55.45

Weight (kDa)

5.98

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 1363
AccII CGCG 1 cut(s) 519
AciI CCGC 4 cut(s) 53, 83, 609, 704
AcoI YGGCCR 1 cut(s) 427
AcsI RAATTY 2 cut(s) 1198, 1440
AcuI CTGAAG 1 cut(s) 469
AcyI GRCGYC 1 cut(s) 311
AfaI GTAC 2 cut(s) 105, 715
AfiI CCNNNNNNNGG 5 cut(s) 58, 59, 86, 291, 1070
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 6 cut(s) 725, 1079, 1179, 1377, 1408, 1451
AjiI CACGTC 1 cut(s) 131
AleI CACNNNNGTG 2 cut(s) 923, 1425
AluBI AGCT 9 cut(s) 222, 267, 385, 394, 500, 583, 601, 700, 826
AluI AGCT 9 cut(s) 222, 267, 385, 394, 500, 583, 601, 700, 826
Alw21I GWGCWC 4 cut(s) 269, 348, 387, 918
Alw26I GTCTC 4 cut(s) 124, 456, 862, 1211
Ama87I CYCGRG 3 cut(s) 23, 386, 593
AoxI GGCC 2 cut(s) 427, 1391
ApeKI GCWGC 2 cut(s) 598, 623
ApoI RAATTY 2 cut(s) 1198, 1440
AspLEI GCGC 2 cut(s) 100, 521
AspS9I GGNCC 1 cut(s) 1300
AsuC2I CCSGG 2 cut(s) 24, 25
AsuHPI GGTGA 3 cut(s) 85, 297, 571
AvaI CYCGRG 3 cut(s) 23, 386, 593
AvaII GGWCC 1 cut(s) 1300
AxyI CCTNAGG 1 cut(s) 852
BalI TGGCCA 1 cut(s) 429
BanI GGYRCC 1 cut(s) 1363
BanII GRGCYC 5 cut(s) 53, 92, 269, 387, 543
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 4 cut(s) 269, 348, 387, 918
BbvI GCAGC 2 cut(s) 610, 610
BccI CCATC 8 cut(s) 157, 182, 283, 380, 408, 419, 853, 959
BceAI ACGGC 1 cut(s) 506
BcnI CCSGG 2 cut(s) 24, 25
BcoDI GTCTC 4 cut(s) 124, 456, 862, 1211
BfaI CTAG 2 cut(s) 501, 639
BfmI CTRYAG 2 cut(s) 967, 1221
BfoI RGCGCY 1 cut(s) 101
BisI GCNGC 3 cut(s) 599, 610, 624
BlsI GCNGC 3 cut(s) 600, 611, 625
Bme1390I CCNGG 2 cut(s) 24, 25
Bme18I GGWCC 1 cut(s) 1300
BmeT110I CYCGRG 3 cut(s) 23, 386, 593
BmgBI CACGTC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 1300
BmiI GGNNCC 2 cut(s) 540, 1365
BmrFI CCNGG 2 cut(s) 24, 25
BmrI ACTGGG 1 cut(s) 726
BmsI GCATC 2 cut(s) 457, 1219
BmuI ACTGGG 1 cut(s) 726
BpiI GAAGAC 1 cut(s) 1125
BpmI CTGGAG 2 cut(s) 516, 557
BpuMI CCSGG 2 cut(s) 24, 25
BsaHI GRCGYC 1 cut(s) 311
BsaI GGTCTC 1 cut(s) 862
BsaJI CCNNGG 3 cut(s) 10, 23, 24
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 59, 86, 291, 1070
Bse1I ACTGG 4 cut(s) 44, 499, 721, 766
Bse21I CCTNAGG 1 cut(s) 852
Bse3DI GCAATG 1 cut(s) 1332
BseDI CCNNGG 3 cut(s) 10, 23, 24
BseGI GGATG 2 cut(s) 122, 1096
BseLI CCNNNNNNNGG 5 cut(s) 58, 59, 86, 291, 1070
BseMI GCAATG 1 cut(s) 1332
BseMII CTCAG 3 cut(s) 293, 866, 1037
BseNI ACTGG 4 cut(s) 44, 499, 721, 766
BseRI GAGGAG 2 cut(s) 474, 840
BseXI GCAGC 2 cut(s) 610, 610
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 2 cut(s) 429, 1393
BshNI GGYRCC 1 cut(s) 1363
BsiHKAI GWGCWC 4 cut(s) 269, 348, 387, 918
BsiHKCI CYCGRG 3 cut(s) 23, 386, 593
BsiSI CCGG 4 cut(s) 24, 71, 80, 95
BslFI GGGAC 3 cut(s) 117, 350, 772
BslI CCNNNNNNNGG 5 cut(s) 58, 59, 86, 291, 1070
BsmAI GTCTC 4 cut(s) 124, 456, 862, 1211
BsmBI CGTCTC 2 cut(s) 124, 456
BsmFI GGGAC 3 cut(s) 117, 350, 772
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 2 cut(s) 429, 1393
Bso31I GGTCTC 1 cut(s) 862
BsoBI CYCGRG 3 cut(s) 23, 386, 593
Bsp1286I GDGCHC 7 cut(s) 53, 92, 269, 348, 387, 543, 918
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 4 cut(s) 53, 83, 609, 704
BspANI GGCC 2 cut(s) 429, 1393
BspCNI CTCAG 3 cut(s) 292, 865, 1036
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 2 cut(s) 540, 1365
BspMAI CTGCAG 1 cut(s) 971
BspT107I GGYRCC 1 cut(s) 1363
BspTNI GGTCTC 1 cut(s) 862
BsrDI GCAATG 1 cut(s) 1332
BsrI ACTGG 4 cut(s) 44, 499, 721, 766
BssECI CCNNGG 3 cut(s) 10, 23, 24
BssNI GRCGYC 1 cut(s) 311
BssT1I CCWWGG 1 cut(s) 10
Bst4CI ACNGT 5 cut(s) 218, 547, 578, 713, 838
BstACI GRCGYC 1 cut(s) 311
BstC8I GCNNGC 2 cut(s) 53, 702
BstDEI CTNAG 5 cut(s) 279, 354, 822, 852, 1023
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 2 cut(s) 122, 1096
BstFNI CGCG 1 cut(s) 519
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 2 cut(s) 100, 521
BstMAI GTCTC 4 cut(s) 124, 456, 862, 1211
BstMWI GCNNNNNNNGC 2 cut(s) 391, 913
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 2 cut(s) 22, 23
BstSFI CTRYAG 2 cut(s) 967, 1221
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 2 cut(s) 610, 610
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 6 cut(s) 189, 532, 673, 685, 764, 776
BstYI RGATCY 6 cut(s) 189, 532, 673, 685, 764, 776
Bsu36I CCTNAGG 1 cut(s) 852
BsuRI GGCC 2 cut(s) 429, 1393
BtgI CCRYGG 1 cut(s) 10
BtrI CACGTC 1 cut(s) 131
BtsCI GGATG 2 cut(s) 122, 1096
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 4 cut(s) 493, 834, 918, 1041
Cac8I GCNNGC 2 cut(s) 53, 702
CfoI GCGC 2 cut(s) 100, 521
Cfr13I GGNCC 1 cut(s) 1300
Cfr9I CCCGGG 1 cut(s) 23
CseI GACGC 3 cut(s) 106, 629, 1056
Csp6I GTAC 2 cut(s) 104, 714
CviAII CATG 9 cut(s) 11, 114, 376, 410, 743, 1195, 1256, 1275, 1387
CviQI GTAC 2 cut(s) 104, 714
DdeI CTNAG 5 cut(s) 279, 354, 822, 852, 1023
EaeI YGGCCR 1 cut(s) 427
Ecl136II GAGCTC 2 cut(s) 267, 385
Eco130I CCWWGG 1 cut(s) 10
Eco147I AGGCCT 1 cut(s) 1393
Eco24I GRGCYC 5 cut(s) 53, 92, 269, 387, 543
Eco31I GGTCTC 1 cut(s) 862
Eco32I GATATC 1 cut(s) 961
Eco47I GGWCC 1 cut(s) 1300
Eco53kI GAGCTC 2 cut(s) 267, 385
Eco57I CTGAAG 1 cut(s) 469
Eco81I CCTNAGG 1 cut(s) 852
Eco88I CYCGRG 3 cut(s) 23, 386, 593
EcoICRI GAGCTC 2 cut(s) 267, 385
EcoRV GATATC 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 10
EcoT22I ATGCAT 1 cut(s) 1257
EcoT38I GRGCYC 5 cut(s) 53, 92, 269, 387, 543
ErhI CCWWGG 1 cut(s) 10
Esp3I CGTCTC 2 cut(s) 124, 456
FaeI CATG 9 cut(s) 14, 117, 379, 413, 746, 1198, 1259, 1278, 1390
FaqI GGGAC 3 cut(s) 117, 350, 772
FatI CATG 9 cut(s) 10, 113, 375, 409, 742, 1194, 1255, 1274, 1386
FauI CCCGC 1 cut(s) 60
Fnu4HI GCNGC 3 cut(s) 599, 610, 624
FokI GGATG 2 cut(s) 109, 1083
FriOI GRGCYC 5 cut(s) 53, 92, 269, 387, 543
Fsp4HI GCNGC 3 cut(s) 599, 610, 624
FspBI CTAG 2 cut(s) 501, 639
GlaI GCGC 2 cut(s) 99, 520
GluI GCNGC 3 cut(s) 599, 610, 624
GsuI CTGGAG 2 cut(s) 516, 557
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 2 cut(s) 429, 1393
HapII CCGG 4 cut(s) 24, 71, 80, 95
HgaI GACGC 3 cut(s) 106, 629, 1056
HhaI GCGC 2 cut(s) 100, 521
Hin1I GRCGYC 1 cut(s) 311
Hin1II CATG 9 cut(s) 14, 117, 379, 413, 746, 1198, 1259, 1278, 1390
Hin6I GCGC 2 cut(s) 98, 519
HinP1I GCGC 2 cut(s) 98, 519
HindIII AAGCTT 2 cut(s) 698, 824
HinfI GANTC 4 cut(s) 76, 184, 831, 1447
HpaII CCGG 4 cut(s) 24, 71, 80, 95
HphI GGTGA 3 cut(s) 85, 297, 571
Hpy166II GTNNAC 2 cut(s) 805, 841
Hpy188I TCNGA 9 cut(s) 48, 163, 183, 189, 690, 752, 1248, 1304, 1354
Hpy188III TCNNGA 8 cut(s) 329, 388, 404, 536, 694, 938, 1313, 1397
Hpy8I GTNNAC 2 cut(s) 805, 841
Hpy99I CGWCG 3 cut(s) 313, 365, 458
HpyAV CCTTC 1 cut(s) 245
HpyCH4III ACNGT 5 cut(s) 218, 547, 578, 713, 838
HpyCH4IV ACGT 3 cut(s) 130, 311, 510
HpyCH4V TGCA 3 cut(s) 969, 1255, 1295
HpyF10VI GCNNNNNNNGC 2 cut(s) 391, 913
HpyF3I CTNAG 5 cut(s) 279, 354, 822, 852, 1023
HpySE526I ACGT 3 cut(s) 130, 311, 510
Hsp92I GRCGYC 1 cut(s) 311
Hsp92II CATG 9 cut(s) 14, 117, 379, 413, 746, 1198, 1259, 1278, 1390
HspAI GCGC 2 cut(s) 98, 519
LmnI GCTCC 3 cut(s) 497, 538, 913
Lsp1109I GCAGC 2 cut(s) 610, 610
LweI GCATC 2 cut(s) 457, 1219
MaeI CTAG 2 cut(s) 501, 639
MaeII ACGT 3 cut(s) 130, 311, 510
MaeIII GTNAC 2 cut(s) 32, 303
MboII GAAGA 7 cut(s) 475, 673, 676, 826, 1125, 1174, 1238
MfeI CAATTG 1 cut(s) 56
MflI RGATCY 6 cut(s) 189, 532, 673, 685, 764, 776
MhlI GDGCHC 7 cut(s) 53, 92, 269, 348, 387, 543, 918
MlsI TGGCCA 1 cut(s) 429
MluNI TGGCCA 1 cut(s) 429
MlyI GAGTC 2 cut(s) 85, 193
MmeI TCCRAC 2 cut(s) 167, 730
Mox20I TGGCCA 1 cut(s) 429
Mph1103I ATGCAT 1 cut(s) 1257
MscI TGGCCA 1 cut(s) 429
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 5 cut(s) 112, 921, 923, 1090, 1425
Msp20I TGGCCA 1 cut(s) 429
MspA1I CMGCKG 1 cut(s) 583
MspI CCGG 4 cut(s) 24, 71, 80, 95
MspR9I CCNGG 2 cut(s) 24, 25
MunI CAATTG 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 414
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 2 cut(s) 391, 913
NciI CCSGG 2 cut(s) 24, 25
NcoI CCATGG 1 cut(s) 10
NlaIII CATG 9 cut(s) 14, 117, 379, 413, 746, 1198, 1259, 1278, 1390
NlaIV GGNNCC 2 cut(s) 540, 1365
NmuCI GTSAC 1 cut(s) 303
NsiI ATGCAT 1 cut(s) 1257
NspI RCATGY 1 cut(s) 117
OliI CACNNNNGTG 2 cut(s) 923, 1425
PaeR7I CTCGAG 1 cut(s) 386
PceI AGGCCT 1 cut(s) 1393
PcsI WCGNNNNNNNCGW 2 cut(s) 308, 462
PctI GAATGC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 831, 1447
PkrI GCNGC 3 cut(s) 600, 611, 625
PleI GAGTC 2 cut(s) 84, 192
PpsI GAGTC 2 cut(s) 84, 192
Psp124BI GAGCTC 2 cut(s) 269, 387
PspN4I GGNNCC 2 cut(s) 540, 1365
PspPI GGNCC 1 cut(s) 1300
PsrI GAACNNNNNNTAC 2 cut(s) 557, 589
PstI CTGCAG 1 cut(s) 971
PsuI RGATCY 6 cut(s) 189, 532, 673, 685, 764, 776
PvuII CAGCTG 1 cut(s) 583
RsaI GTAC 2 cut(s) 105, 715
RsaNI GTAC 2 cut(s) 104, 714
RseI CAYNNNNRTG 5 cut(s) 112, 921, 923, 1090, 1425
SacI GAGCTC 2 cut(s) 269, 387
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 3 cut(s) 599, 610, 624
Sau96I GGNCC 1 cut(s) 1300
SchI GAGTC 2 cut(s) 85, 193
ScrFI CCNGG 2 cut(s) 24, 25
SduI GDGCHC 7 cut(s) 53, 92, 269, 348, 387, 543, 918
SfaNI GCATC 2 cut(s) 457, 1219
SfcI CTRYAG 2 cut(s) 967, 1221
Sfr274I CTCGAG 1 cut(s) 386
SinI GGWCC 1 cut(s) 1300
SlaI CTCGAG 1 cut(s) 386
SmaI CCCGGG 1 cut(s) 25
SmiMI CAYNNNNRTG 5 cut(s) 112, 921, 923, 1090, 1425
SmlI CTYRAG 2 cut(s) 386, 1471
SmoI CTYRAG 2 cut(s) 386, 1471
SseBI AGGCCT 1 cut(s) 1393
SsiI CCGC 4 cut(s) 53, 83, 609, 704
SspI AATATT 1 cut(s) 325
SspMI CTAG 2 cut(s) 501, 639
SstI GAGCTC 2 cut(s) 269, 387
StuI AGGCCT 1 cut(s) 1393
StyD4I CCNGG 2 cut(s) 22, 23
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 5 cut(s) 218, 547, 578, 713, 838
TaiI ACGT 3 cut(s) 133, 314, 513
TaqI TCGA 3 cut(s) 387, 453, 552
TatI WGTACW 1 cut(s) 713
TauI GCSGC 1 cut(s) 612
TfiI GAWTC 2 cut(s) 831, 1447
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 4 cut(s) 493, 841, 925, 1048
TseFI GTSAC 1 cut(s) 303
TseI GCWGC 2 cut(s) 598, 623
Tsp45I GTSAC 1 cut(s) 303
TspDTI ATGAA 4 cut(s) 960, 1211, 1263, 1272
TspGWI ACGGA 2 cut(s) 471, 582
TspMI CCCGGG 1 cut(s) 23
TspRI CASTG 4 cut(s) 493, 841, 925, 1048
VpaK11BI GGWCC 1 cut(s) 1300
XapI RAATTY 2 cut(s) 1198, 1440
XceI RCATGY 1 cut(s) 117
XcmI CCANNNNNNNNNTGG 1 cut(s) 407
XhoI CTCGAG 1 cut(s) 386
XmaI CCCGGG 1 cut(s) 23
XspI CTAG 2 cut(s) 501, 639
ZraI GACGTC 1 cut(s) 312
Zsp2I ATGCAT 1 cut(s) 1257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.