Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001673

Physical Location & Seq

Genomic Coordinates Forward (+)

52320506 .. 52321483

Gene Structure 978 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1221000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 978 bp

ATGAAGGAGAAGGGGCTAGAGTTAAATGTAATTCTTTATAACACCCTTTTAGCTATGTGTGCTGATGTTGGTAACACTGACGAAGCCGTTGACATTTTTGAAGAAATGAAGAGTTCTGAAACCTTGAAGCCCGACAGTTGGACCTTTTCATCCATGATCACCATATATTCCTGTAGCGGGAAAGTCACAGAGGCAGAAGCAACATTGAATGAGATGTTGGAGGCTGGTTTTGAACCTAATATCTTTATTTTGACATCACTCATCCAATGCTACGGGAAGGCAAAACGCACTGATGACGTTGTTAGGATATTCAATCAACTCCTAGATTTGGGTATAACACCAGATGAGCGTTTCTGTGGTTGTCTCCTGAATGTGATGACTCAAACACCAAAGGAAGAACTTTGCAAGCTTGCCAATTGCATTGAGAGGGCTGATGAAAAACTAGGGTATGTGGTAAGACTTTTGGTGGAGAAGCAAGACAGTAGTGAAAACTTCAAGAATGAAGCATCAGAACTTTTCAACTCAATCGGTTCGGATGTAAAGAAGGCTTATTGCAATTGCCTGATTGATCTTTGTGTGAACCTCGATCTATTGGAGAGAGCCTGTGAGCTATTAGACTTGGGGATTACACTTCAGATATACATAGGTTTACAGTCAAGGTCTCAAACCCAGTGGTCTCTGTATCTCAAAGGTCTGTCTCTTGGGGCCGCTCTGACTGCATTACATGTTTGGATCAATGACCTATCTCGGGTATTGGACTCTGCTGAGGAGCTTCCACCGTTGCTTGGAATCAATACTGGACATGGGAAACATAAGTATTCAGACAAGGGTCTGGCAAGTGTATTTGAGTCCCATTTGAAGGAATTAAATGCTCCATTCCATGAGGCCCCGGACAAGGCTGGCTGGTTTTTGACGACAAAGGTTGCAATCAAGTCATGGTTGGAGTCTAGGAGTTCATCCGAGTTAGTTACTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016070 GO:0016787 GO:0016788 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031425 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

326

Amino Acids

36.17

Weight (kDa)

4.82

Isoelectric Point (pI)

32.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_3	PF13812	1 - 56	1.3e-10	Pentatricopeptide repeat domain
PPR_long	PF17177	1 - 133	6e-10	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	4 - 37	2.1e-06	PPR repeat
PPR_2	PF13041	9 - 54	7.6e-11	PPR repeat family
PPR	PF01535	12 - 39	1e-05	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001673)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16390
fragaria_vesca	FvH4_3g00440 FvH4_3g00470
malus_domestica	MD00G1221000.v1.1 MD05G1360400.v1.1 MD08G1234300.v1.1 MD10G1337400.v1.1 MD10G1337800.v1.1 MD10G1338000.v1.1
prunus_persica	Prupe.3G138500_v2.0.a1 Prupe.4G005200_v2.0.a1
pyrus_communis	pycom05g32720 pycom10g28680 pycom10g28700 pycom10g28720 pycom10g28750
rosa_chinensis	RchiOBHm_Chr1g0364151 RchiOBHm_Chr2g0155431 RchiOBHm_Chr4g0402181 RchiOBHm_Chr5g0000631 RchiOBHm_Chr6g0271321
rosa_laevigata	RLG00000004254 RLG00000013858 RLG00000028931 RLG00000030883
rosa_multiflora	Rmu_co8004710.1_g000001 Rmu_co8021096.1_g000001 Rmu_co8161794.1_g000001 Rmu_sc0000795.1_g000110 Rmu_sc0001141.1_g000016 Rmu_sc0003662.1_g000007 Rmu_sc0003699.1_g000009 Rmu_sc0006695.1_g000081 Rmu_sc0010255.1_g000007 Rmu_sc0011992.1_g000014 Rmu_sc0016929.1_g000002 Rmu_sc0017523.1_g000008 Rmu_sc0021532.1_g000001 Rmu_sc0029877.1_g000001
rosa_roxburghii	Rroxscaffold_1G00001310 Rroxscaffold_1G00075570 Rroxscaffold_4G00324290
rosa_rugosa	Rorug04G0385500 Rorug07G0309300.1
rosa_samantha	Rh5AG005400 Rh5BG007000 Rh5CG005700 Rh5DG005600
rosa_wichuraiana	Rw5G000530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	39
AccBSI	CCGCTC	1 cut(s)	710
AciI	CCGC	2 cut(s)	177, 708
AclWI	GGATC	1 cut(s)	740
AcuI	CTGAAG	1 cut(s)	617
AfiI	CCNNNNNNNGG	7 cut(s)	138, 177, 328, 748, 785, 859, 895
AflIII	ACRYGT	1 cut(s)	724
AgsI	TTSAA	8 cut(s)	101, 127, 208, 233, 313, 496, 520, 859
AjuI	GAANNNNNNNTTGG	2 cut(s)	200, 232
AluBI	AGCT	4 cut(s)	53, 409, 610, 772
AluI	AGCT	4 cut(s)	53, 409, 610, 772
Alw26I	GTCTC	4 cut(s)	368, 666, 681, 702
AlwI	GGATC	1 cut(s)	740
Ama87I	CYCGRG	1 cut(s)	747
AoxI	GGCC	2 cut(s)	705, 885
AspS9I	GGNCC	3 cut(s)	141, 705, 886
AsuC2I	CCSGG	1 cut(s)	890
AsuHPI	GGTGA	1 cut(s)	151
AvaI	CYCGRG	1 cut(s)	747
AvaII	GGWCC	1 cut(s)	141
BbvCI	CCTCAGC	1 cut(s)	765
BceAI	ACGGC	1 cut(s)	71
BclI	TGATCA	1 cut(s)	156
BcnI	CCSGG	1 cut(s)	890
BcoDI	GTCTC	4 cut(s)	368, 666, 681, 702
BfaI	CTAG	4 cut(s)	17, 323, 443, 948
BfmI	CTRYAG	1 cut(s)	172
BisI	GCNGC	1 cut(s)	708
BlsI	GCNGC	1 cut(s)	709
Bme1390I	CCNGG	1 cut(s)	890
Bme18I	GGWCC	1 cut(s)	141
BmeT110I	CYCGRG	1 cut(s)	747
BmgT120I	GGNCC	3 cut(s)	141, 705, 886
BmiI	GGNNCC	2 cut(s)	706, 888
BmrFI	CCNGG	1 cut(s)	890
BmrI	ACTGGG	1 cut(s)	664
BmsI	GCATC	1 cut(s)	515
BmuI	ACTGGG	1 cut(s)	664
BoxI	GACNNNNGTC	1 cut(s)	828
Bpu10I	CCTNAGC	1 cut(s)	765
BpuMI	CCSGG	1 cut(s)	890
BsaI	GGTCTC	2 cut(s)	666, 681
BsaJI	CCNNGG	1 cut(s)	888
Bsc4I	CCNNNNNNNGG	7 cut(s)	138, 177, 328, 748, 785, 859, 895
Bse1I	ACTGG	2 cut(s)	670, 802
BseDI	CCNNGG	1 cut(s)	888
BseGI	GGATG	4 cut(s)	149, 261, 541, 956
BseLI	CCNNNNNNNGG	7 cut(s)	138, 177, 328, 748, 785, 859, 895
BseMII	CTCAG	1 cut(s)	756
BseNI	ACTGG	2 cut(s)	670, 802
BseRI	GAGGAG	1 cut(s)	782
BshFI	GGCC	2 cut(s)	707, 887
BsiHKCI	CYCGRG	1 cut(s)	747
BsiSI	CCGG	1 cut(s)	890
BslFI	GGGAC	1 cut(s)	835
BslI	CCNNNNNNNGG	7 cut(s)	138, 177, 328, 748, 785, 859, 895
BsmAI	GTCTC	4 cut(s)	368, 666, 681, 702
BsmFI	GGGAC	1 cut(s)	835
BsnI	GGCC	2 cut(s)	707, 887
Bso31I	GGTCTC	2 cut(s)	666, 681
BsoBI	CYCGRG	1 cut(s)	747
Bsp143I	GATC	4 cut(s)	156, 568, 586, 732
BspACI	CCGC	2 cut(s)	177, 708
BspANI	GGCC	2 cut(s)	707, 887
BspCNI	CTCAG	1 cut(s)	757
BspLI	GGNNCC	2 cut(s)	706, 888
BspPI	GGATC	1 cut(s)	740
BspTNI	GGTCTC	2 cut(s)	666, 681
BsrBI	CCGCTC	1 cut(s)	710
BsrI	ACTGG	2 cut(s)	670, 802
BssECI	CCNNGG	1 cut(s)	888
BssMI	GATC	4 cut(s)	156, 568, 586, 732
Bst4CI	ACNGT	4 cut(s)	137, 482, 654, 780
Bst6I	CTCTTC	1 cut(s)	104
BstC8I	GCNNGC	3 cut(s)	407, 411, 901
BstDEI	CTNAG	2 cut(s)	765, 975
BstF5I	GGATG	4 cut(s)	149, 261, 541, 956
BstKTI	GATC	4 cut(s)	159, 571, 589, 735
BstMAI	GTCTC	4 cut(s)	368, 666, 681, 702
BstMBI	GATC	4 cut(s)	156, 568, 586, 732
BstMWI	GCNNNNNNNGC	2 cut(s)	59, 716
BstNSI	RCATGY	1 cut(s)	728
BstPAI	GACNNNNGTC	1 cut(s)	828
BstSCI	CCNGG	1 cut(s)	888
BstSFI	CTRYAG	1 cut(s)	172
BsuRI	GGCC	2 cut(s)	707, 887
BtsCI	GGATG	4 cut(s)	149, 261, 541, 956
BtsIMutI	CAGTG	3 cut(s)	75, 288, 677
Cac8I	GCNNGC	3 cut(s)	407, 411, 901
Cfr13I	GGNCC	3 cut(s)	141, 705, 886
CspCI	CAANNNNNGTGG	2 cut(s)	653, 688
CviAII	CATG	5 cut(s)	154, 725, 803, 881, 936
DdeI	CTNAG	2 cut(s)	765, 975
DpnI	GATC	4 cut(s)	158, 570, 588, 734
DpnII	GATC	4 cut(s)	156, 568, 586, 732
Eam1104I	CTCTTC	1 cut(s)	104
EarI	CTCTTC	1 cut(s)	104
Eco31I	GGTCTC	2 cut(s)	666, 681
Eco47I	GGWCC	1 cut(s)	141
Eco57I	CTGAAG	1 cut(s)	617
Eco88I	CYCGRG	1 cut(s)	747
EcoO109I	RGGNCCY	1 cut(s)	886
FaeI	CATG	5 cut(s)	157, 728, 806, 884, 939
FaqI	GGGAC	1 cut(s)	835
FatI	CATG	5 cut(s)	153, 724, 802, 880, 935
FauI	CCCGC	1 cut(s)	170
FbaI	TGATCA	1 cut(s)	156
Fnu4HI	GCNGC	1 cut(s)	708
FokI	GGATG	4 cut(s)	136, 248, 548, 943
Fsp4HI	GCNGC	1 cut(s)	708
FspBI	CTAG	4 cut(s)	17, 323, 443, 948
GluI	GCNGC	1 cut(s)	708
HaeIII	GGCC	2 cut(s)	707, 887
HapII	CCGG	1 cut(s)	890
Hin1II	CATG	5 cut(s)	157, 728, 806, 884, 939
HincII	GTYRAC	1 cut(s)	91
HindII	GTYRAC	1 cut(s)	91
HindIII	AAGCTT	1 cut(s)	407
HinfI	GANTC	5 cut(s)	379, 758, 789, 848, 944
HpaII	CCGG	1 cut(s)	890
HphI	GGTGA	1 cut(s)	151
Hpy166II	GTNNAC	3 cut(s)	91, 580, 650
Hpy188I	TCNGA	7 cut(s)	118, 511, 535, 636, 714, 823, 961
Hpy188III	TCNNGA	2 cut(s)	367, 496
Hpy8I	GTNNAC	3 cut(s)	91, 580, 650
HpyAV	CCTTC	4 cut(s)	4, 271, 538, 853
HpyCH4III	ACNGT	4 cut(s)	137, 482, 654, 780
HpyCH4IV	ACGT	1 cut(s)	297
HpyCH4V	TGCA	5 cut(s)	405, 420, 555, 719, 926
HpyF10VI	GCNNNNNNNGC	2 cut(s)	59, 716
HpyF3I	CTNAG	2 cut(s)	765, 975
HpySE526I	ACGT	1 cut(s)	297
Hsp92II	CATG	5 cut(s)	157, 728, 806, 884, 939
Ksp22I	TGATCA	1 cut(s)	156
Kzo9I	GATC	4 cut(s)	156, 568, 586, 732
LmnI	GCTCC	2 cut(s)	769, 877
LweI	GCATC	1 cut(s)	515
MaeI	CTAG	4 cut(s)	17, 323, 443, 948
MaeII	ACGT	1 cut(s)	297
MaeIII	GTNAC	3 cut(s)	71, 184, 967
MalI	GATC	4 cut(s)	158, 570, 588, 734
MbiI	CCGCTC	1 cut(s)	710
MboI	GATC	4 cut(s)	156, 568, 586, 732
MboII	GAAGA	3 cut(s)	113, 121, 407
MfeI	CAATTG	2 cut(s)	415, 556
MluCI	AATT	4 cut(s)	30, 415, 556, 863
MlyI	GAGTC	4 cut(s)	373, 752, 857, 953
MmeI	TCCRAC	3 cut(s)	119, 198, 921
MnlI	CCTC	6 cut(s)	184, 214, 420, 593, 760, 877
MseI	TTAA	2 cut(s)	23, 866
MspI	CCGG	1 cut(s)	890
MspR9I	CCNGG	1 cut(s)	890
MunI	CAATTG	2 cut(s)	415, 556
MwoI	GCNNNNNNNGC	2 cut(s)	59, 716
NciI	CCSGG	1 cut(s)	890
NdeII	GATC	4 cut(s)	156, 568, 586, 732
NlaIII	CATG	5 cut(s)	157, 728, 806, 884, 939
NlaIV	GGNNCC	2 cut(s)	706, 888
NmuCI	GTSAC	1 cut(s)	184
NspI	RCATGY	1 cut(s)	728
PciI	ACATGT	1 cut(s)	724
PfeI	GAWTC	1 cut(s)	789
PkrI	GCNGC	1 cut(s)	709
PleI	GAGTC	4 cut(s)	373, 752, 856, 952
PpsI	GAGTC	4 cut(s)	373, 752, 856, 952
PscI	ACATGT	1 cut(s)	724
PshAI	GACNNNNGTC	1 cut(s)	828
PsiI	TTATAA	1 cut(s)	39
PspN4I	GGNNCC	2 cut(s)	706, 888
PspPI	GGNCC	3 cut(s)	141, 705, 886
SaqAI	TTAA	2 cut(s)	23, 866
SatI	GCNGC	1 cut(s)	708
Sau3AI	GATC	4 cut(s)	156, 568, 586, 732
Sau96I	GGNCC	3 cut(s)	141, 705, 886
SchI	GAGTC	4 cut(s)	373, 752, 857, 953
ScrFI	CCNGG	1 cut(s)	890
SfaNI	GCATC	1 cut(s)	515
SfcI	CTRYAG	1 cut(s)	172
SinI	GGWCC	1 cut(s)	141
Sse9I	AATT	4 cut(s)	30, 415, 556, 863
SsiI	CCGC	2 cut(s)	177, 708
SspMI	CTAG	4 cut(s)	17, 323, 443, 948
StyD4I	CCNGG	1 cut(s)	888
TaaI	ACNGT	4 cut(s)	137, 482, 654, 780
TaiI	ACGT	1 cut(s)	300
TaqI	TCGA	1 cut(s)	585
TasI	AATT	4 cut(s)	30, 415, 556, 863
TauI	GCSGC	1 cut(s)	710
TfiI	GAWTC	1 cut(s)	789
Tru1I	TTAA	2 cut(s)	23, 866
Tru9I	TTAA	2 cut(s)	23, 866
TscAI	CASTG	3 cut(s)	82, 295, 677
TseFI	GTSAC	1 cut(s)	184
Tsp45I	GTSAC	1 cut(s)	184
TspDTI	ATGAA	6 cut(s)	17, 122, 138, 450, 516, 945
TspRI	CASTG	3 cut(s)	82, 295, 677
VpaK11BI	GGWCC	1 cut(s)	141
XceI	RCATGY	1 cut(s)	728
XspI	CTAG	4 cut(s)	17, 323, 443, 948

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.