Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 288 |
| AccB1I | GGYRCC | 1 cut(s) | 769 |
| AccIII | TCCGGA | 1 cut(s) | 99 |
| AciI | CCGC | 3 cut(s) | 42, 110, 373 |
| AclWI | GGATC | 6 cut(s) | 91, 99, 104, 178, 190, 461 |
| AcsI | RAATTY | 2 cut(s) | 604, 846 |
| AfaI | GTAC | 1 cut(s) | 121 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 476 |
| AgsI | TTSAA | 5 cut(s) | 131, 485, 585, 783, 814 |
| AleI | CACNNNNGTG | 2 cut(s) | 329, 831 |
| AluBI | AGCT | 4 cut(s) | 19, 106, 227, 232 |
| AluI | AGCT | 4 cut(s) | 19, 106, 227, 232 |
| Alw21I | GWGCWC | 1 cut(s) | 324 |
| Alw26I | GTCTC | 2 cut(s) | 268, 617 |
| AlwI | GGATC | 6 cut(s) | 91, 99, 104, 178, 190, 461 |
| Aor13HI | TCCGGA | 1 cut(s) | 99 |
| AoxI | GGCC | 1 cut(s) | 61 |
| ApeKI | GCWGC | 1 cut(s) | 16 |
| ApoI | RAATTY | 2 cut(s) | 604, 846 |
| AseI | ATTAAT | 1 cut(s) | 525 |
| AspS9I | GGNCC | 1 cut(s) | 706 |
| AvaII | GGWCC | 1 cut(s) | 706 |
| AxyI | CCTNAGG | 1 cut(s) | 258 |
| BamHI | GGATCC | 1 cut(s) | 96 |
| BanI | GGYRCC | 1 cut(s) | 769 |
| Bbv12I | GWGCWC | 1 cut(s) | 324 |
| BbvI | GCAGC | 1 cut(s) | 28 |
| BccI | CCATC | 2 cut(s) | 259, 365 |
| BceAI | ACGGC | 1 cut(s) | 137 |
| BcoDI | GTCTC | 2 cut(s) | 268, 617 |
| BisI | GCNGC | 2 cut(s) | 17, 373 |
| BlpI | GCTNAGC | 1 cut(s) | 228 |
| BlsI | GCNGC | 2 cut(s) | 18, 374 |
| Bme18I | GGWCC | 1 cut(s) | 706 |
| BmgT120I | GGNCC | 1 cut(s) | 706 |
| BmiI | GGNNCC | 2 cut(s) | 98, 771 |
| BmrI | ACTGGG | 1 cut(s) | 132 |
| BmsI | GCATC | 1 cut(s) | 625 |
| BmuI | ACTGGG | 1 cut(s) | 132 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 521, 553 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 228 |
| BsaI | GGTCTC | 1 cut(s) | 268 |
| BsaWI | WCCGGW | 1 cut(s) | 99 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 476 |
| Bse1I | ACTGG | 2 cut(s) | 127, 172 |
| Bse21I | CCTNAGG | 1 cut(s) | 258 |
| Bse3DI | GCAATG | 1 cut(s) | 738 |
| BseAI | TCCGGA | 1 cut(s) | 99 |
| BseGI | GGATG | 1 cut(s) | 502 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 476 |
| BseMI | GCAATG | 1 cut(s) | 738 |
| BseMII | CTCAG | 2 cut(s) | 272, 443 |
| BseNI | ACTGG | 2 cut(s) | 127, 172 |
| BseRI | GAGGAG | 1 cut(s) | 246 |
| BseXI | GCAGC | 1 cut(s) | 28 |
| BshFI | GGCC | 1 cut(s) | 63 |
| BshNI | GGYRCC | 1 cut(s) | 769 |
| BsiHKAI | GWGCWC | 1 cut(s) | 324 |
| BsiSI | CCGG | 1 cut(s) | 100 |
| BslFI | GGGAC | 2 cut(s) | 130, 178 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 476 |
| BsmAI | GTCTC | 2 cut(s) | 268, 617 |
| BsmFI | GGGAC | 2 cut(s) | 130, 178 |
| BsnI | GGCC | 1 cut(s) | 63 |
| Bso31I | GGTCTC | 1 cut(s) | 268 |
| Bsp1286I | GDGCHC | 1 cut(s) | 324 |
| Bsp13I | TCCGGA | 1 cut(s) | 99 |
| Bsp143I | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 228 |
| BspACI | CCGC | 3 cut(s) | 42, 110, 373 |
| BspANI | GGCC | 1 cut(s) | 63 |
| BspCNI | CTCAG | 2 cut(s) | 271, 442 |
| BspEI | TCCGGA | 1 cut(s) | 99 |
| BspLI | GGNNCC | 2 cut(s) | 98, 771 |
| BspPI | GGATC | 6 cut(s) | 91, 99, 104, 178, 190, 461 |
| BspT107I | GGYRCC | 1 cut(s) | 769 |
| BspTNI | GGTCTC | 1 cut(s) | 268 |
| BsrDI | GCAATG | 1 cut(s) | 738 |
| BsrI | ACTGG | 2 cut(s) | 127, 172 |
| BssMI | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| Bst4CI | ACNGT | 3 cut(s) | 119, 244, 293 |
| Bst6I | CTCTTC | 1 cut(s) | 544 |
| BstC8I | GCNNGC | 2 cut(s) | 108, 311 |
| BstDEI | CTNAG | 3 cut(s) | 228, 258, 429 |
| BstF5I | GGATG | 1 cut(s) | 502 |
| BstKTI | GATC | 5 cut(s) | 94, 99, 173, 185, 469 |
| BstMAI | GTCTC | 2 cut(s) | 268, 617 |
| BstMBI | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 319 |
| BstV1I | GCAGC | 1 cut(s) | 28 |
| BstX2I | RGATCY | 3 cut(s) | 96, 170, 182 |
| BstYI | RGATCY | 3 cut(s) | 96, 170, 182 |
| Bsu36I | CCTNAGG | 1 cut(s) | 258 |
| BsuRI | GGCC | 1 cut(s) | 63 |
| BtsCI | GGATG | 1 cut(s) | 502 |
| BtsI | GCAGTG | 1 cut(s) | 447 |
| BtsIMutI | CAGTG | 5 cut(s) | 31, 240, 298, 324, 447 |
| Cac8I | GCNNGC | 2 cut(s) | 108, 311 |
| Cfr13I | GGNCC | 1 cut(s) | 706 |
| Csp6I | GTAC | 1 cut(s) | 120 |
| CviAII | CATG | 4 cut(s) | 149, 601, 681, 793 |
| CviQI | GTAC | 1 cut(s) | 120 |
| DdeI | CTNAG | 3 cut(s) | 228, 258, 429 |
| DpnI | GATC | 5 cut(s) | 93, 98, 172, 184, 468 |
| DpnII | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| Eam1104I | CTCTTC | 1 cut(s) | 544 |
| EarI | CTCTTC | 1 cut(s) | 544 |
| Eco31I | GGTCTC | 1 cut(s) | 268 |
| Eco32I | GATATC | 1 cut(s) | 367 |
| Eco47I | GGWCC | 1 cut(s) | 706 |
| Eco81I | CCTNAGG | 1 cut(s) | 258 |
| EcoRV | GATATC | 1 cut(s) | 367 |
| FaeI | CATG | 4 cut(s) | 152, 604, 684, 796 |
| FaiI | YATR | 9 cut(s) | 71, 150, 216, 288, 363, 602, 682, 747, 794 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 216, 248 |
| FaqI | GGGAC | 2 cut(s) | 130, 178 |
| FatI | CATG | 4 cut(s) | 148, 600, 680, 792 |
| Fnu4HI | GCNGC | 2 cut(s) | 17, 373 |
| FokI | GGATG | 1 cut(s) | 489 |
| Fsp4HI | GCNGC | 2 cut(s) | 17, 373 |
| GluI | GCNGC | 2 cut(s) | 17, 373 |
| HaeIII | GGCC | 1 cut(s) | 63 |
| HapII | CCGG | 1 cut(s) | 100 |
| Hin1II | CATG | 4 cut(s) | 152, 604, 684, 796 |
| HindIII | AAGCTT | 2 cut(s) | 104, 230 |
| HinfI | GANTC | 2 cut(s) | 237, 796 |
| HpaII | CCGG | 1 cut(s) | 100 |
| Hpy166II | GTNNAC | 3 cut(s) | 211, 247, 303 |
| Hpy188I | TCNGA | 4 cut(s) | 96, 158, 654, 760 |
| Hpy188III | TCNNGA | 5 cut(s) | 100, 344, 470, 719, 803 |
| Hpy8I | GTNNAC | 3 cut(s) | 211, 247, 303 |
| Hpy99I | CGWCG | 1 cut(s) | 158 |
| HpyAV | CCTTC | 1 cut(s) | 73 |
| HpyCH4III | ACNGT | 3 cut(s) | 119, 244, 293 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 319 |
| HpyF3I | CTNAG | 3 cut(s) | 228, 258, 429 |
| Hsp92II | CATG | 4 cut(s) | 152, 604, 684, 796 |
| Kpn2I | TCCGGA | 1 cut(s) | 99 |
| Kzo9I | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| LmnI | GCTCC | 1 cut(s) | 319 |
| Lsp1109I | GCAGC | 1 cut(s) | 28 |
| LweI | GCATC | 1 cut(s) | 625 |
| MalI | GATC | 5 cut(s) | 93, 98, 172, 184, 468 |
| MboI | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| MboII | GAAGA | 4 cut(s) | 232, 531, 580, 644 |
| MflI | RGATCY | 3 cut(s) | 96, 170, 182 |
| MhlI | GDGCHC | 1 cut(s) | 324 |
| MluCI | AATT | 8 cut(s) | 72, 522, 604, 613, 644, 711, 778, 846 |
| MmeI | TCCRAC | 1 cut(s) | 136 |
| MroI | TCCGGA | 1 cut(s) | 99 |
| MseI | TTAA | 3 cut(s) | 525, 555, 822 |
| MslI | CAYNNNNRTG | 4 cut(s) | 327, 329, 496, 831 |
| MspI | CCGG | 1 cut(s) | 100 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 319 |
| NdeII | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| NlaIII | CATG | 4 cut(s) | 152, 604, 684, 796 |
| NlaIV | GGNNCC | 2 cut(s) | 98, 771 |
| OliI | CACNNNNGTG | 2 cut(s) | 329, 831 |
| PfeI | GAWTC | 2 cut(s) | 237, 796 |
| PkrI | GCNGC | 2 cut(s) | 18, 374 |
| PshBI | ATTAAT | 1 cut(s) | 525 |
| PsiI | TTATAA | 1 cut(s) | 288 |
| PspN4I | GGNNCC | 2 cut(s) | 98, 771 |
| PspPI | GGNCC | 1 cut(s) | 706 |
| PsuI | RGATCY | 3 cut(s) | 96, 170, 182 |
| RsaI | GTAC | 1 cut(s) | 121 |
| RsaNI | GTAC | 1 cut(s) | 120 |
| RseI | CAYNNNNRTG | 4 cut(s) | 327, 329, 496, 831 |
| SaqAI | TTAA | 3 cut(s) | 525, 555, 822 |
| SatI | GCNGC | 2 cut(s) | 17, 373 |
| Sau3AI | GATC | 5 cut(s) | 91, 96, 170, 182, 466 |
| Sau96I | GGNCC | 1 cut(s) | 706 |
| SduI | GDGCHC | 1 cut(s) | 324 |
| SfaNI | GCATC | 1 cut(s) | 625 |
| SinI | GGWCC | 1 cut(s) | 706 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 327, 329, 496, 831 |
| Sse9I | AATT | 8 cut(s) | 72, 522, 604, 613, 644, 711, 778, 846 |
| SsiI | CCGC | 3 cut(s) | 42, 110, 373 |
| TaaI | ACNGT | 3 cut(s) | 119, 244, 293 |
| TasI | AATT | 8 cut(s) | 72, 522, 604, 613, 644, 711, 778, 846 |
| TatI | WGTACW | 1 cut(s) | 119 |
| TauI | GCSGC | 1 cut(s) | 375 |
| TfiI | GAWTC | 2 cut(s) | 237, 796 |
| Tru1I | TTAA | 3 cut(s) | 525, 555, 822 |
| Tru9I | TTAA | 3 cut(s) | 525, 555, 822 |
| TscAI | CASTG | 5 cut(s) | 31, 247, 298, 331, 454 |
| TseI | GCWGC | 1 cut(s) | 16 |
| TspDTI | ATGAA | 3 cut(s) | 366, 617, 669 |
| TspRI | CASTG | 5 cut(s) | 31, 247, 298, 331, 454 |
| VpaK11BI | GGWCC | 1 cut(s) | 706 |
| VspI | ATTAAT | 1 cut(s) | 525 |
| XapI | RAATTY | 2 cut(s) | 604, 846 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 71 |