Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0001673

Physical Location & Seq

Genomic Coordinates Reverse (-)

30137209 .. 30139439

Gene Structure 2231 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1234300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 954 bp

ATGAACAATCCGGAAAATGCTTTGCTTGCGCTTAACTACTTTCAGCAGAGGTTGAAACCGAAGAGGGAGGTGATTTTGTATAATGTGACTTTAAAAGTGTGTAGGAAGGGTAAGAATTTGGAGAGAGCAGAGAAGCTGTTCGATGAAATACTCCAGAGAGGTGTTAAACCCAATAATGTTACCTTTTCGACTTCGATTAGTTGTGCTGGGATGTGTTCTTTGCCTGATAAGGCAGTGGAGTGGTTTGAGAAGATGCCCAGTTTTGGGTGTAATCCGGATGATGTTACCTATTCGGCTATGATTGATGCCTATGGACGTGCAGGTAAGGTTGAAATGGCTTTCAGTTTGTATGACCGGTCCAGGACTGAGAAGTGGCGCATTGATCCAGTGACGTTTTCTACATTGATCAAAATTCACGGGCAATCGGGGAATTTTGATGGGTGCTTGAATGTTTATGAGGAAATGAAAGCTATAGCGGCTAAGCCAAACTTGGCAGAAGCAACATTGAATGAGATGTTGGAAGCTGATTTTGAGCCAAATACCTTTATTTTGACATCACTCGTCCAATTCTACGGGAAGGCAAAACGCACTGATGACGTTGTTAAGATATTCAATCAACTCCTAGAATTGGGTATAACACCAGATGAGCGTTTCTGTGGTTGTCTCCTGAATGTGATGACTCAAACACCAAAGGAAGAACTTTGCAAGCTTGTGAATTGCATTGAGAGGGCTGATGAAAAACTAGGGTATGTGGTAAGACTTTTAGTGGAGAAGCAAGAAAGTAGTGAAAACTTCAAGAACGAAGCATCACAACTTTTTAACTCGATTGGTTCGGATGTAAAGAAGGCTTACTGCAATTGCCTGATTGATCTTTGTGTGAACCTCAATCTCTTGGAGAGAGCCTGTGAGCTACTAGACTTGGGGATCACACTTCAGATATATATAGGCTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006417 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016070 GO:0016787 GO:0016788 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031425 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045727 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:2000112

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

318

Amino Acids

35.89

Weight (kDa)

5.3

Isoelectric Point (pI)

28.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	24 - 67	1.6e-08	PPR repeat family
PPR_long	PF17177	26 - 166	6.4e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	75 - 104	2.7e-07	PPR repeat family
PPR_1	PF12854	89 - 118	2e-08	PPR repeat
PPR_2	PF13041	95 - 137	1.1e-07	PPR repeat family
PPR	PF01535	95 - 119	3.7e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0001673)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16390
fragaria_vesca	FvH4_3g00440 FvH4_3g00470
malus_domestica	MD00G1221000.v1.1 MD05G1360400.v1.1 MD08G1234300.v1.1 MD10G1337400.v1.1 MD10G1337800.v1.1 MD10G1338000.v1.1
prunus_persica	Prupe.3G138500_v2.0.a1 Prupe.4G005200_v2.0.a1
pyrus_communis	pycom05g32720 pycom10g28680 pycom10g28700 pycom10g28720 pycom10g28750
rosa_chinensis	RchiOBHm_Chr1g0364151 RchiOBHm_Chr2g0155431 RchiOBHm_Chr4g0402181 RchiOBHm_Chr5g0000631 RchiOBHm_Chr6g0271321
rosa_laevigata	RLG00000004254 RLG00000013858 RLG00000028931 RLG00000030883
rosa_multiflora	Rmu_co8004710.1_g000001 Rmu_co8021096.1_g000001 Rmu_co8161794.1_g000001 Rmu_sc0000795.1_g000110 Rmu_sc0001141.1_g000016 Rmu_sc0003662.1_g000007 Rmu_sc0003699.1_g000009 Rmu_sc0006695.1_g000081 Rmu_sc0010255.1_g000007 Rmu_sc0011992.1_g000014 Rmu_sc0016929.1_g000002 Rmu_sc0017523.1_g000008 Rmu_sc0021532.1_g000001 Rmu_sc0029877.1_g000001
rosa_roxburghii	Rroxscaffold_1G00001310 Rroxscaffold_1G00075570 Rroxscaffold_4G00324290
rosa_rugosa	Rorug04G0385500 Rorug07G0309300.1
rosa_samantha	Rh5AG005400 Rh5BG007000 Rh5CG005700 Rh5DG005600
rosa_wichuraiana	Rw5G000530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	311
AccIII	TCCGGA	2 cut(s)	10, 274
AciI	CCGC	1 cut(s)	476
AclWI	GGATC	2 cut(s)	377, 932
AcsI	RAATTY	3 cut(s)	115, 411, 430
AcuI	CTGAAG	1 cut(s)	917
AfiI	CCNNNNNNNGG	3 cut(s)	263, 264, 628
AgeI	ACCGGT	1 cut(s)	354
AgsI	TTSAA	6 cut(s)	55, 332, 448, 508, 613, 796
AjiI	CACGTC	1 cut(s)	317
AjnI	CCWGG	1 cut(s)	359
AjuI	GAANNNNNNNTTGG	2 cut(s)	500, 532
AluBI	AGCT	5 cut(s)	136, 470, 524, 709, 910
AluI	AGCT	5 cut(s)	136, 470, 524, 709, 910
Alw26I	GTCTC	1 cut(s)	668
AlwI	GGATC	2 cut(s)	377, 932
Aor13HI	TCCGGA	2 cut(s)	10, 274
ApoI	RAATTY	3 cut(s)	115, 411, 430
AsiGI	ACCGGT	1 cut(s)	354
Asp700I	GAANNNNTTC	1 cut(s)	137
AspLEI	GCGC	2 cut(s)	31, 378
AspS9I	GGNCC	1 cut(s)	357
AsuHPI	GGTGA	1 cut(s)	82
AvaII	GGWCC	1 cut(s)	357
BccI	CCATC	1 cut(s)	431
BciT130I	CCWGG	1 cut(s)	361
BclI	TGATCA	1 cut(s)	405
BcoDI	GTCTC	1 cut(s)	668
BfaI	CTAG	3 cut(s)	623, 743, 914
BfmI	CTRYAG	1 cut(s)	471
BfuAI	ACCTGC	1 cut(s)	311
BisI	GCNGC	1 cut(s)	477
BlpI	GCTNAGC	1 cut(s)	480
BlsI	GCNGC	1 cut(s)	478
Bme1390I	CCNGG	1 cut(s)	361
Bme18I	GGWCC	1 cut(s)	357
BmgBI	CACGTC	1 cut(s)	317
BmgT120I	GGNCC	1 cut(s)	357
BmrFI	CCNGG	1 cut(s)	361
BmrI	ACTGGG	1 cut(s)	252
BmsI	GCATC	3 cut(s)	243, 295, 815
BmuI	ACTGGG	1 cut(s)	252
BpmI	CTGGAG	1 cut(s)	137
Bpu1102I	GCTNAGC	1 cut(s)	480
BsaWI	WCCGGW	3 cut(s)	10, 274, 354
Bsc4I	CCNNNNNNNGG	3 cut(s)	263, 264, 628
Bse118I	RCCGGY	1 cut(s)	354
Bse1I	ACTGG	2 cut(s)	258, 386
BseAI	TCCGGA	2 cut(s)	10, 274
BseBI	CCWGG	1 cut(s)	361
BseGI	GGATG	3 cut(s)	216, 283, 841
BseLI	CCNNNNNNNGG	3 cut(s)	263, 264, 628
BseMII	CTCAG	1 cut(s)	357
BseNI	ACTGG	2 cut(s)	258, 386
BseYI	CCCAGC	1 cut(s)	206
BsgI	GTGCAG	1 cut(s)	339
BshTI	ACCGGT	1 cut(s)	354
BsiSI	CCGG	3 cut(s)	11, 275, 355
BslI	CCNNNNNNNGG	3 cut(s)	263, 264, 628
BsmAI	GTCTC	1 cut(s)	668
Bsp13I	TCCGGA	2 cut(s)	10, 274
Bsp143I	GATC	4 cut(s)	382, 405, 868, 924
Bsp1720I	GCTNAGC	1 cut(s)	480
BspACI	CCGC	1 cut(s)	476
BspCNI	CTCAG	1 cut(s)	358
BspEI	TCCGGA	2 cut(s)	10, 274
BspMI	ACCTGC	1 cut(s)	311
BspPI	GGATC	2 cut(s)	377, 932
BsrFI	RCCGGY	1 cut(s)	354
BsrI	ACTGG	2 cut(s)	258, 386
BssAI	RCCGGY	1 cut(s)	354
BssMI	GATC	4 cut(s)	382, 405, 868, 924
Bst2UI	CCWGG	1 cut(s)	361
Bst6I	CTCTTC	1 cut(s)	56
BstC8I	GCNNGC	2 cut(s)	27, 707
BstDEI	CTNAG	2 cut(s)	366, 480
BstF5I	GGATG	3 cut(s)	216, 283, 841
BstHHI	GCGC	2 cut(s)	31, 378
BstKTI	GATC	4 cut(s)	385, 408, 871, 927
BstMAI	GTCTC	1 cut(s)	668
BstMBI	GATC	4 cut(s)	382, 405, 868, 924
BstMWI	GCNNNNNNNGC	2 cut(s)	26, 476
BstNI	CCWGG	1 cut(s)	361
BstSCI	CCNGG	1 cut(s)	359
BstSFI	CTRYAG	1 cut(s)	471
BtrI	CACGTC	1 cut(s)	317
BtsCI	GGATG	3 cut(s)	216, 283, 841
BtsI	GCAGTG	1 cut(s)	240
BtsIMutI	CAGTG	3 cut(s)	240, 393, 588
BveI	ACCTGC	1 cut(s)	311
Cac8I	GCNNGC	2 cut(s)	27, 707
CfoI	GCGC	2 cut(s)	31, 378
Cfr10I	RCCGGY	1 cut(s)	354
Cfr13I	GGNCC	1 cut(s)	357
CspAI	ACCGGT	1 cut(s)	354
DdeI	CTNAG	2 cut(s)	366, 480
DpnI	GATC	4 cut(s)	384, 407, 870, 926
DpnII	GATC	4 cut(s)	382, 405, 868, 924
DraI	TTTAAA	1 cut(s)	93
Eam1104I	CTCTTC	1 cut(s)	56
EarI	CTCTTC	1 cut(s)	56
Eco47I	GGWCC	1 cut(s)	357
Eco57I	CTGAAG	1 cut(s)	917
EcoRII	CCWGG	1 cut(s)	359
FalI	AAGNNNNNCTT	2 cut(s)	473, 505
FbaI	TGATCA	1 cut(s)	405
Fnu4HI	GCNGC	1 cut(s)	477
FokI	GGATG	3 cut(s)	223, 290, 848
Fsp4HI	GCNGC	1 cut(s)	477
FspBI	CTAG	3 cut(s)	623, 743, 914
GlaI	GCGC	2 cut(s)	30, 377
GluI	GCNGC	1 cut(s)	477
GsaI	CCCAGC	1 cut(s)	210
GsuI	CTGGAG	1 cut(s)	137
HapII	CCGG	3 cut(s)	11, 275, 355
HhaI	GCGC	2 cut(s)	31, 378
Hin6I	GCGC	2 cut(s)	29, 376
HinP1I	GCGC	2 cut(s)	29, 376
HindIII	AAGCTT	1 cut(s)	707
HinfI	GANTC	1 cut(s)	679
HpaII	CCGG	3 cut(s)	11, 275, 355
HphI	GGTGA	1 cut(s)	82
Hpy166II	GTNNAC	1 cut(s)	880
Hpy188I	TCNGA	2 cut(s)	835, 936
Hpy188III	TCNNGA	5 cut(s)	11, 154, 275, 667, 796
Hpy8I	GTNNAC	1 cut(s)	880
HpyAV	CCTTC	3 cut(s)	100, 571, 838
HpyCH4IV	ACGT	3 cut(s)	316, 392, 597
HpyCH4V	TGCA	4 cut(s)	320, 705, 720, 855
HpyF10VI	GCNNNNNNNGC	2 cut(s)	26, 476
HpyF3I	CTNAG	2 cut(s)	366, 480
HpySE526I	ACGT	3 cut(s)	316, 392, 597
HspAI	GCGC	2 cut(s)	29, 376
Kpn2I	TCCGGA	2 cut(s)	10, 274
Ksp22I	TGATCA	1 cut(s)	405
Kzo9I	GATC	4 cut(s)	382, 405, 868, 924
LweI	GCATC	3 cut(s)	243, 295, 815
MaeI	CTAG	3 cut(s)	623, 743, 914
MaeII	ACGT	3 cut(s)	316, 392, 597
MaeIII	GTNAC	4 cut(s)	85, 178, 283, 388
MalI	GATC	4 cut(s)	384, 407, 870, 926
MboI	GATC	4 cut(s)	382, 405, 868, 924
MboII	GAAGA	3 cut(s)	73, 262, 707
MfeI	CAATTG	1 cut(s)	856
MluCI	AATT	7 cut(s)	115, 411, 430, 566, 626, 715, 856
MlyI	GAGTC	1 cut(s)	673
MmeI	TCCRAC	1 cut(s)	498
MnlI	CCTC	7 cut(s)	42, 57, 61, 152, 451, 720, 893
MroI	TCCGGA	2 cut(s)	10, 274
MroXI	GAANNNNTTC	1 cut(s)	137
MseI	TTAA	5 cut(s)	33, 92, 165, 603, 819
MspI	CCGG	3 cut(s)	11, 275, 355
MspR9I	CCNGG	1 cut(s)	361
MunI	CAATTG	1 cut(s)	856
MvaI	CCWGG	1 cut(s)	361
MwoI	GCNNNNNNNGC	2 cut(s)	26, 476
NdeII	GATC	4 cut(s)	382, 405, 868, 924
NmuCI	GTSAC	2 cut(s)	85, 388
PdmI	GAANNNNTTC	1 cut(s)	137
PfoI	TCCNGGA	1 cut(s)	359
PinAI	ACCGGT	1 cut(s)	354
PkrI	GCNGC	1 cut(s)	478
PleI	GAGTC	1 cut(s)	673
PpsI	GAGTC	1 cut(s)	673
Psp6I	CCWGG	1 cut(s)	359
PspFI	CCCAGC	1 cut(s)	206
PspGI	CCWGG	1 cut(s)	359
PspPI	GGNCC	1 cut(s)	357
SaqAI	TTAA	5 cut(s)	33, 92, 165, 603, 819
SatI	GCNGC	1 cut(s)	477
Sau3AI	GATC	4 cut(s)	382, 405, 868, 924
Sau96I	GGNCC	1 cut(s)	357
SchI	GAGTC	1 cut(s)	673
ScrFI	CCNGG	1 cut(s)	361
SfaNI	GCATC	3 cut(s)	243, 295, 815
SfcI	CTRYAG	1 cut(s)	471
SinI	GGWCC	1 cut(s)	357
Sse9I	AATT	7 cut(s)	115, 411, 430, 566, 626, 715, 856
SsiI	CCGC	1 cut(s)	476
SspMI	CTAG	3 cut(s)	623, 743, 914
StyD4I	CCNGG	1 cut(s)	359
TaiI	ACGT	3 cut(s)	319, 395, 600
TaqI	TCGA	4 cut(s)	141, 188, 194, 824
TasI	AATT	7 cut(s)	115, 411, 430, 566, 626, 715, 856
TauI	GCSGC	1 cut(s)	479
Tru1I	TTAA	5 cut(s)	33, 92, 165, 603, 819
Tru9I	TTAA	5 cut(s)	33, 92, 165, 603, 819
TscAI	CASTG	3 cut(s)	240, 393, 595
TseFI	GTSAC	2 cut(s)	85, 388
Tsp45I	GTSAC	2 cut(s)	85, 388
TspDTI	ATGAA	4 cut(s)	17, 159, 479, 750
TspRI	CASTG	3 cut(s)	240, 393, 595
VpaK11BI	GGWCC	1 cut(s)	357
XapI	RAATTY	3 cut(s)	115, 411, 430
XmnI	GAANNNNTTC	1 cut(s)	137
XspI	CTAG	3 cut(s)	623, 743, 914

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.