FvH4_3g42040

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000923
Physical Location & Seq
Reverse (-)
35151608 .. 35155598
3991 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42040.t3

Sequence Viewer

Length: 2325 bp
ATGGTGCTTTCATCTCTTTCACCCTTGCTATTATTATCAGCAGTCTTGTTTTCTCTATTGCAGACACCTACCCTTTCCATAGAAAAGCCATATATAGTGTACTTGGGAGCACATTCTCATGGTCCAAACCCATCTTCGGTTGATCTCGGCTACGTCAGAAATTTTCACTACGATTTTCTGGGGTCATTCTTGAGAAGCAATAGAAGTGCCAAAGATGTGATTTTCTACTCCTATACTAAACACATTAATGGTTTTGCTGCAATTCTTGAAGAAGAAGAAGCTGCTCAGATTGCTAAAGATCCAAATGTGATATCAGTTTTCCTAAACAAGGGAAAACAGCTGCAAACTACGCGGTCATGGAATTTTCTCGGACTAGAGAGAAATGGACTAATTCCTTCAGACTCCATTTGGAAGAAATCAAGATTTGGTGAAGATACAATAATTGCAAACATTGATACTGGTGTTTGGCCAGAATCAAAAAGCTTTAGTGATGAAGGATTGGGACCTGTCCCTTCCAAGTGGCATGGAACTTGTCAACGTGACACAAAGCAAGTTCAATGCAACAGAAAGCTGATTGGAACTAGGTACTTCAACAATGGTCTTGCCATGTATGCCGGCCCTCTCAACTCGTCATATTCCACTGTCCGGGACCATGATGGTCATGGATCACATACCTTATCCACGGCTGCAGGTGGTTTTGTTCCAGGAGTTAGTGTTTTTGGCAATGGCAATGGAACCGCCAAGGGTGGCTCACCTCAAGCACGTGTTGCTGCATATAAGGTATGCTGGCCAACATTTGAAGGTGTTCAATGCTCTGAGGCAGACATCTTAGCTGCCTTTGATGCTGCAATAAGTGATGGTGTCGATGTAATCTCCGTGTCTCTTGGTGGAGGAACAGAGGAGTATTTCAACGATGCTATCTCAATAGGGTCCTTCCATGCAGTTAAAAATGGCATTGTTGTGGTAAGTGCAGCTGGAAACTCTGGACCAAATCCGGGGACAGTATTAAACTTGTCGCCATGGTTAGTGACAGTCGGCGCTAGCACAGTTGATCGAGAGTTCACAAGCTCTGTTTTCCTCGGAAACAAAAAGCAATTCAAGGGAGCAAGCCTTTCAGCAAAAGACTTACCATCTGAAATGTTTTACCCGTTGATCAGTGCTGCAGATGCGAGAAATGCTAATGCATCCACTAGAGAAGCTGAAGTTTGCAATGTTGGTGCCCTTGATCCTAGGAAAGTAAACGGAAAGATTTTGGTTTGCCTTCGAGAATACAACAATAATGAAAGAACCGAAAAAAGCCAGCAGGCTGATATTGCAGGTGCTTTAGGAATGATCTTGGTAAATGATGAGCAAAGTGGAAATGATGTTGTAGCCGATCCTCATGTGCTCCTTGTTTCGCATCTCAACTATAGCGATGGCAAACATGTCTTTGATTACATTAAATCCACCAAGCTTCCTGTGGCTTACCTAACTCCAGTAAAGGCTGAATTGGGAGTGAGGCCAGCTCCAACTGTAGCTCCATTTTCATCACGGGGGCCTAATCTTCTGGAGCAGGCAATCTTAAAGCCTGATATCATTGCACCAGGGGTGAGTATAATCGCTGCTTATACTGAAGCAACAGGGCCAACTTATCAATCATCTGATACTCGTCGCGTTTCTTTTAATGTTCAAACCGGCACTTCAATGGCGTGCCCTCATGTATCCGGAGTTGCAGGGCTTTTGAGAACACTGCATCCAGATTGGAGTCCAGCAGTTATTAAATCTGCAATCATGACAACTGCTACAACACAAGATGACAGCAATGAACAGATGTTTGACTCGTCTGCTTATCTTAAGGCAACACCATTTGCTTATGGTGCAGGACACATTCAACCAAACAAGGCTATGGACCCAGGACTAATCTATAACCTAACAACACTTGATTACTTGGATCTCTTATGTGCTCGAGGATACAACGAAACAATGATCAAATCATTTTCTGATTCACCTTTTAAATGTCCCAAGTCTTTCAGTTTAGGAGACTTCAACTATCCAGCAATTGTAGTTCCTAACCTCGGTGCAACATTAGTAACCATTACTAGAAGAAGAGTTACAAATGTCGGATCACCTGGCACGTACAAAGTGCGCGTCAAGGCACCACCAGAAGTAGAGGTTTTGGTTAAACCTAGAAGCTTGAAGTTTGAGAGTATTGGCGAAGTGAAAAAGTTTGAAGTCATATTCAAAGCCAAGGTTAAGGGTAAGCCTCGAGGGTATGCATTTGGAGAGTTGATGTGGTCAGATGGCAATCACCATGTAAAGACTCCTCTTGCGGTGAAGCACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

775

Amino Acids

83.47

Weight (kDa)

8.17

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 111 1.8e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 593 5.6e-39 Subtilase family
PA PF02225 394 - 474 1.4e-09 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 680, 1307
AbsI CCTCGAGG 1 cut(s) 2244
Acc36I ACCTGC 2 cut(s) 680, 1307
AccB1I GGYRCC 2 cut(s) 1217, 2134
AccII CGCG 3 cut(s) 352, 1653, 2127
AccIII TCCGGA 1 cut(s) 1703
AciI CCGC 3 cut(s) 352, 738, 2309
AclWI GGATC 6 cut(s) 293, 673, 1220, 1370, 1938, 2110
AcoI YGGCCR 2 cut(s) 467, 788
AcsI RAATTY 2 cut(s) 160, 361
AcuI CTGAAG 3 cut(s) 381, 1221, 1632
AcvI CACGTG 1 cut(s) 764
AfaI GTAC 3 cut(s) 101, 587, 2117
AfiI CCNNNNNNNGG 5 cut(s) 136, 328, 645, 995, 2054
AflII CTTAAG 1 cut(s) 1832
AflIII ACRYGT 2 cut(s) 763, 1423
AjnI CCWGG 4 cut(s) 703, 1582, 1891, 2107
AjuI GAANNNNNNNTTGG 2 cut(s) 118, 150
AloI GAACNNNNNNTCC 2 cut(s) 1717, 1749
Alw21I GWGCWC 3 cut(s) 112, 1389, 1945
Alw26I GTCTC 2 cut(s) 885, 2013
AlwI GGATC 6 cut(s) 293, 673, 1220, 1370, 1938, 2110
Ama87I CYCGRG 2 cut(s) 1944, 2244
Aor13HI TCCGGA 1 cut(s) 1703
AoxI GGCC 6 cut(s) 467, 616, 788, 1499, 1535, 1622
ApoI RAATTY 2 cut(s) 160, 361
ArsI GACNNNNNNTTYG 2 cut(s) 2112, 2144
AseI ATTAAT 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 804
AspA2I CCTAGG 1 cut(s) 1229
AspLEI GCGC 2 cut(s) 1040, 2127
AspS9I GGNCC 9 cut(s) 122, 503, 617, 649, 930, 986, 1535, 1622, 1888
AsuC2I CCSGG 2 cut(s) 647, 996
AsuHPI GGTGA 8 cut(s) 12, 440, 744, 1600, 1977, 2097, 2279, 2323
AsuNHI GCTAGC 1 cut(s) 1040
AvaI CYCGRG 2 cut(s) 1944, 2244
AvaII GGWCC 6 cut(s) 122, 503, 649, 930, 986, 1888
AvrII CCTAGG 1 cut(s) 1229
BaeGI GKGCMC 2 cut(s) 1222, 1694
BaeI ACNNNNGTAYC 2 cut(s) 447, 480
BalI TGGCCA 2 cut(s) 469, 790
BanI GGYRCC 2 cut(s) 1217, 2134
BarI GAAGNNNNNNTAC 2 cut(s) 2074, 2106
BbrPI CACGTG 1 cut(s) 764
Bbv12I GWGCWC 3 cut(s) 112, 1389, 1945
BccI CCATC 6 cut(s) 139, 650, 851, 1138, 1409, 2273
BceAI ACGGC 1 cut(s) 699
BciT130I CCWGG 4 cut(s) 705, 1584, 1893, 2109
BciVI GTATCC 2 cut(s) 1711, 1943
BclI TGATCA 2 cut(s) 1152, 1965
BcnI CCSGG 2 cut(s) 647, 996
BcoDI GTCTC 2 cut(s) 885, 2013
BfaI CTAG 8 cut(s) 374, 582, 1041, 1191, 1230, 2079, 2166, 2323
BfmI CTRYAG 4 cut(s) 687, 1161, 1408, 1512
BfoI RGCGCY 1 cut(s) 1041
BfrI CTTAAG 1 cut(s) 1832
BfuAI ACCTGC 2 cut(s) 680, 1307
BfuI GTATCC 2 cut(s) 1711, 1943
BlnI CCTAGG 1 cut(s) 1229
Bme1390I CCNGG 6 cut(s) 647, 705, 996, 1584, 1893, 2109
Bme18I GGWCC 6 cut(s) 122, 503, 649, 930, 986, 1888
BmeT110I CYCGRG 2 cut(s) 1944, 2244
BmgT120I GGNCC 9 cut(s) 122, 503, 617, 649, 930, 986, 1535, 1622, 1888
BmiI GGNNCC 8 cut(s) 504, 650, 736, 931, 1219, 1536, 1890, 2136
BmrFI CCNGG 6 cut(s) 647, 705, 996, 1584, 1893, 2109
BmsI GCATC 6 cut(s) 832, 904, 1156, 1193, 1408, 1741
BmtI GCTAGC 1 cut(s) 1044
BplI GAGNNNNNCTC 2 cut(s) 1489, 1521
BpmI CTGGAG 2 cut(s) 1458, 1568
BpuEI CTTGAG 2 cut(s) 211, 741
BpuMI CCSGG 2 cut(s) 647, 996
BsaAI YACGTR 2 cut(s) 764, 2115
BsaBI GATNNNNATC 1 cut(s) 2283
BsaWI WCCGGW 1 cut(s) 1703
BsaXI ACNNNNNCTCC 4 cut(s) 699, 729, 1501, 1531
Bsc4I CCNNNNNNNGG 5 cut(s) 136, 328, 645, 995, 2054
Bse118I RCCGGY 2 cut(s) 614, 1673
Bse1I ACTGG 2 cut(s) 463, 1475
Bse3DI GCAATG 5 cut(s) 730, 736, 1216, 1575, 1807
Bse8I GATNNNNATC 1 cut(s) 2283
BseAI TCCGGA 1 cut(s) 1703
BseBI CCWGG 4 cut(s) 705, 1584, 1893, 2109
BseGI GGATG 2 cut(s) 1184, 1732
BseJI GATNNNNATC 1 cut(s) 2283
BseLI CCNNNNNNNGG 5 cut(s) 136, 328, 645, 995, 2054
BseMI GCAATG 5 cut(s) 730, 736, 1216, 1575, 1807
BseMII CTCAG 2 cut(s) 299, 807
BseNI ACTGG 2 cut(s) 463, 1475
BseRI GAGGAG 2 cut(s) 914, 2292
BseSI GKGCMC 2 cut(s) 1222, 1694
BsgI GTGCAG 2 cut(s) 990, 1878
Bsh1236I CGCG 3 cut(s) 352, 1653, 2127
BshFI GGCC 6 cut(s) 469, 618, 790, 1501, 1537, 1624
BshNI GGYRCC 2 cut(s) 1217, 2134
BsiHKAI GWGCWC 3 cut(s) 112, 1389, 1945
BsiHKCI CYCGRG 2 cut(s) 1944, 2244
BsiSI CCGG 5 cut(s) 615, 646, 995, 1674, 1704
BslFI GGGAC 5 cut(s) 494, 516, 662, 1012, 1983
BslI CCNNNNNNNGG 5 cut(s) 136, 328, 645, 995, 2054
BsmAI GTCTC 2 cut(s) 885, 2013
BsmFI GGGAC 5 cut(s) 494, 516, 662, 1012, 1983
BsnI GGCC 6 cut(s) 469, 618, 790, 1501, 1537, 1624
BsoBI CYCGRG 2 cut(s) 1944, 2244
Bsp1286I GDGCHC 5 cut(s) 112, 1222, 1389, 1694, 1945
Bsp13I TCCGGA 1 cut(s) 1703
Bsp19I CCATGG 1 cut(s) 1019
BspACI CCGC 3 cut(s) 352, 738, 2309
BspANI GGCC 6 cut(s) 469, 618, 790, 1501, 1537, 1624
BspCNI CTCAG 2 cut(s) 298, 808
BspEI TCCGGA 1 cut(s) 1703
BspFNI CGCG 3 cut(s) 352, 1653, 2127
BspHI TCATGA 1 cut(s) 1770
BspLI GGNNCC 8 cut(s) 504, 650, 736, 931, 1219, 1536, 1890, 2136
BspMAI CTGCAG 2 cut(s) 691, 1165
BspMI ACCTGC 2 cut(s) 680, 1307
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 6 cut(s) 293, 673, 1220, 1370, 1938, 2110
BspT107I GGYRCC 2 cut(s) 1217, 2134
BspTI CTTAAG 1 cut(s) 1832
BsrDI GCAATG 5 cut(s) 730, 736, 1216, 1575, 1807
BsrFI RCCGGY 2 cut(s) 614, 1673
BsrI ACTGG 2 cut(s) 463, 1475
BssAI RCCGGY 2 cut(s) 614, 1673
BssT1I CCWWGG 4 cut(s) 741, 1019, 1229, 2226
Bst2UI CCWGG 4 cut(s) 705, 1584, 1893, 2109
Bst4CI ACNGT 5 cut(s) 643, 1003, 1033, 1048, 1513
Bst6I CTCTTC 1 cut(s) 2080
BstAFI CTTAAG 1 cut(s) 1832
BstAPI GCANNNNNTGC 1 cut(s) 767
BstBAI YACGTR 2 cut(s) 764, 2115
BstC8I GCNNGC 9 cut(s) 616, 788, 1042, 1108, 1301, 1305, 1503, 1554, 1690
BstDEI CTNAG 3 cut(s) 285, 816, 829
BstDSI CCRYGG 2 cut(s) 681, 1019
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 1184, 1732
BstFNI CGCG 3 cut(s) 352, 1653, 2127
BstH2I RGCGCY 1 cut(s) 1041
BstHHI GCGC 2 cut(s) 1040, 2127
BstMAI GTCTC 2 cut(s) 885, 2013
BstMWI GCNNNNNNNGC 9 cut(s) 290, 349, 611, 767, 842, 1166, 1175, 1313, 1856
BstNI CCWGG 4 cut(s) 705, 1584, 1893, 2109
BstNSI RCATGY 1 cut(s) 1427
BstSCI CCNGG 6 cut(s) 645, 703, 994, 1582, 1891, 2107
BstSFI CTRYAG 4 cut(s) 687, 1161, 1408, 1512
BstSLI GKGCMC 2 cut(s) 1222, 1694
BstUI CGCG 3 cut(s) 352, 1653, 2127
BstX2I RGATCY 2 cut(s) 298, 1930
BstYI RGATCY 2 cut(s) 298, 1930
BsuI GTATCC 2 cut(s) 1711, 1943
BsuRI GGCC 6 cut(s) 469, 618, 790, 1501, 1537, 1624
BtgI CCRYGG 2 cut(s) 681, 1019
BtgZI GCGATG 1 cut(s) 1428
BtsCI GGATG 2 cut(s) 1184, 1732
BtsI GCAGTG 1 cut(s) 1727
BtsIMutI CAGTG 3 cut(s) 639, 1162, 1727
BveI ACCTGC 2 cut(s) 680, 1307
Cac8I GCNNGC 9 cut(s) 616, 788, 1042, 1108, 1301, 1305, 1503, 1554, 1690
CciI TCATGA 1 cut(s) 1770
CfoI GCGC 2 cut(s) 1040, 2127
Cfr10I RCCGGY 2 cut(s) 614, 1673
Cfr13I GGNCC 9 cut(s) 122, 503, 617, 649, 930, 986, 1535, 1622, 1888
CseI GACGC 1 cut(s) 2116
Csp6I GTAC 3 cut(s) 100, 586, 2116
CviQI GTAC 3 cut(s) 100, 586, 2116
DdeI CTNAG 3 cut(s) 285, 816, 829
DraI TTTAAA 1 cut(s) 1993
EaeI YGGCCR 2 cut(s) 467, 788
Eam1104I CTCTTC 1 cut(s) 2080
EarI CTCTTC 1 cut(s) 2080
Eco130I CCWWGG 4 cut(s) 741, 1019, 1229, 2226
Eco32I GATATC 2 cut(s) 312, 1573
Eco47I GGWCC 6 cut(s) 122, 503, 649, 930, 986, 1888
Eco57I CTGAAG 3 cut(s) 381, 1221, 1632
Eco72I CACGTG 1 cut(s) 764
Eco88I CYCGRG 2 cut(s) 1944, 2244
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoO109I RGGNCCY 3 cut(s) 503, 930, 1535
EcoRII CCWGG 4 cut(s) 703, 1582, 1891, 2107
EcoRV GATATC 2 cut(s) 312, 1573
EcoT14I CCWWGG 4 cut(s) 741, 1019, 1229, 2226
EcoT22I ATGCAT 2 cut(s) 1186, 2257
ErhI CCWWGG 4 cut(s) 741, 1019, 1229, 2226
FaqI GGGAC 5 cut(s) 494, 516, 662, 1012, 1983
FbaI TGATCA 2 cut(s) 1152, 1965
FokI GGATG 2 cut(s) 1171, 1719
FspBI CTAG 8 cut(s) 374, 582, 1041, 1191, 1230, 2079, 2166, 2323
GlaI GCGC 2 cut(s) 1039, 2126
GsuI CTGGAG 2 cut(s) 1458, 1568
HaeII RGCGCY 1 cut(s) 1041
HaeIII GGCC 6 cut(s) 469, 618, 790, 1501, 1537, 1624
HapII CCGG 5 cut(s) 615, 646, 995, 1674, 1704
HgaI GACGC 1 cut(s) 2116
HhaI GCGC 2 cut(s) 1040, 2127
Hin6I GCGC 2 cut(s) 1038, 2125
HinP1I GCGC 2 cut(s) 1038, 2125
HincII GTYRAC 1 cut(s) 536
HindII GTYRAC 1 cut(s) 536
HindIII AAGCTT 3 cut(s) 481, 1451, 2170
HinfI GANTC 6 cut(s) 401, 473, 1744, 1817, 1982, 2299
HpaII CCGG 5 cut(s) 615, 646, 995, 1674, 1704
HphI GGTGA 8 cut(s) 12, 440, 744, 1600, 1977, 2097, 2279, 2323
Hpy166II GTNNAC 4 cut(s) 100, 536, 1062, 1240
Hpy8I GTNNAC 4 cut(s) 100, 536, 1062, 1240
Hpy99I CGWCG 1 cut(s) 1653
HpyAV CCTTC 6 cut(s) 405, 488, 522, 794, 943, 1271
HpyCH4III ACNGT 5 cut(s) 643, 1003, 1033, 1048, 1513
HpyCH4IV ACGT 4 cut(s) 153, 538, 763, 2114
HpyF10VI GCNNNNNNNGC 9 cut(s) 290, 349, 611, 767, 842, 1166, 1175, 1313, 1856
HpyF3I CTNAG 3 cut(s) 285, 816, 829
HpySE526I ACGT 4 cut(s) 153, 538, 763, 2114
HspAI GCGC 2 cut(s) 1038, 2125
Kpn2I TCCGGA 1 cut(s) 1703
KroI GCCGGC 1 cut(s) 614
KroNI GCCGGC 1 cut(s) 616
Ksp22I TGATCA 2 cut(s) 1152, 1965
LmnI GCTCC 6 cut(s) 107, 1103, 1392, 1510, 1522, 1549
LweI GCATC 6 cut(s) 832, 904, 1156, 1193, 1408, 1741
MaeI CTAG 8 cut(s) 374, 582, 1041, 1191, 1230, 2079, 2166, 2323
MaeII ACGT 4 cut(s) 153, 538, 763, 2114
MaeIII GTNAC 4 cut(s) 539, 1027, 2068, 2089
MboII GAAGA 9 cut(s) 126, 281, 284, 287, 424, 443, 1535, 2094, 2097
MfeI CAATTG 1 cut(s) 2037
MflI RGATCY 2 cut(s) 298, 1930
MhlI GDGCHC 5 cut(s) 112, 1222, 1389, 1694, 1945
MlsI TGGCCA 2 cut(s) 469, 790
MluCI AATT 8 cut(s) 160, 261, 361, 390, 441, 1094, 1487, 2037
MluNI TGGCCA 2 cut(s) 469, 790
MlyI GAGTC 4 cut(s) 395, 1753, 1811, 2293
MmeI TCCRAC 2 cut(s) 1532, 2080
Mox20I TGGCCA 2 cut(s) 469, 790
Mph1103I ATGCAT 2 cut(s) 1186, 2257
MroI TCCGGA 1 cut(s) 1703
MroNI GCCGGC 1 cut(s) 614
MroXI GAANNNNTTC 1 cut(s) 804
MscI TGGCCA 2 cut(s) 469, 790
MslI CAYNNNNRTG 4 cut(s) 117, 246, 959, 1682
Msp20I TGGCCA 2 cut(s) 469, 790
MspA1I CMGCKG 2 cut(s) 340, 974
MspCI CTTAAG 1 cut(s) 1832
MspI CCGG 5 cut(s) 615, 646, 995, 1674, 1704
MspR9I CCNGG 6 cut(s) 647, 705, 996, 1584, 1893, 2109
MunI CAATTG 1 cut(s) 2037
MvaI CCWGG 4 cut(s) 705, 1584, 1893, 2109
MvnI CGCG 3 cut(s) 352, 1653, 2127
MwoI GCNNNNNNNGC 9 cut(s) 290, 349, 611, 767, 842, 1166, 1175, 1313, 1856
NaeI GCCGGC 1 cut(s) 616
NciI CCSGG 2 cut(s) 647, 996
NcoI CCATGG 1 cut(s) 1019
NgoMIV GCCGGC 1 cut(s) 614
NheI GCTAGC 1 cut(s) 1040
NlaIV GGNNCC 8 cut(s) 504, 650, 736, 931, 1219, 1536, 1890, 2136
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 2 cut(s) 539, 1027
NsiI ATGCAT 2 cut(s) 1186, 2257
NspI RCATGY 1 cut(s) 1427
PaeR7I CTCGAG 2 cut(s) 1944, 2244
PagI TCATGA 1 cut(s) 1770
PaqCI CACCTGC 2 cut(s) 680, 1307
PciI ACATGT 1 cut(s) 1423
PdiI GCCGGC 1 cut(s) 616
PdmI GAANNNNTTC 1 cut(s) 804
PfeI GAWTC 2 cut(s) 473, 1982
PfoI TCCNGGA 2 cut(s) 645, 703
PleI GAGTC 4 cut(s) 395, 1752, 1811, 2293
PmaCI CACGTG 1 cut(s) 764
PmlI CACGTG 1 cut(s) 764
PpsI GAGTC 4 cut(s) 395, 1752, 1811, 2293
Ppu21I YACGTR 2 cut(s) 764, 2115
PpuMI RGGWCCY 2 cut(s) 503, 930
PscI ACATGT 1 cut(s) 1423
PshBI ATTAAT 1 cut(s) 246
Psp5II RGGWCCY 2 cut(s) 503, 930
Psp6I CCWGG 4 cut(s) 703, 1582, 1891, 2107
PspCI CACGTG 1 cut(s) 764
PspGI CCWGG 4 cut(s) 703, 1582, 1891, 2107
PspN4I GGNNCC 8 cut(s) 504, 650, 736, 931, 1219, 1536, 1890, 2136
PspPI GGNCC 9 cut(s) 122, 503, 617, 649, 930, 986, 1535, 1622, 1888
PspPPI RGGWCCY 2 cut(s) 503, 930
PspXI VCTCGAGB 2 cut(s) 1944, 2244
PstI CTGCAG 2 cut(s) 691, 1165
PsuI RGATCY 2 cut(s) 298, 1930
PvuII CAGCTG 2 cut(s) 340, 974
RsaI GTAC 3 cut(s) 101, 587, 2117
RsaNI GTAC 3 cut(s) 100, 586, 2116
RseI CAYNNNNRTG 4 cut(s) 117, 246, 959, 1682
Sau96I GGNCC 9 cut(s) 122, 503, 617, 649, 930, 986, 1535, 1622, 1888
SchI GAGTC 4 cut(s) 395, 1753, 1811, 2293
ScrFI CCNGG 6 cut(s) 647, 705, 996, 1584, 1893, 2109
SduI GDGCHC 5 cut(s) 112, 1222, 1389, 1694, 1945
SfaNI GCATC 6 cut(s) 832, 904, 1156, 1193, 1408, 1741
SfcI CTRYAG 4 cut(s) 687, 1161, 1408, 1512
Sfr274I CTCGAG 2 cut(s) 1944, 2244
SinI GGWCC 6 cut(s) 122, 503, 649, 930, 986, 1888
SlaI CTCGAG 2 cut(s) 1944, 2244
SmiMI CAYNNNNRTG 4 cut(s) 117, 246, 959, 1682
SmlI CTYRAG 5 cut(s) 190, 756, 1832, 1944, 2244
SmoI CTYRAG 5 cut(s) 190, 756, 1832, 1944, 2244
Sse9I AATT 8 cut(s) 160, 261, 361, 390, 441, 1094, 1487, 2037
SsiI CCGC 3 cut(s) 352, 738, 2309
SspMI CTAG 8 cut(s) 374, 582, 1041, 1191, 1230, 2079, 2166, 2323
StyD4I CCNGG 6 cut(s) 645, 703, 994, 1582, 1891, 2107
StyI CCWWGG 4 cut(s) 741, 1019, 1229, 2226
TaaI ACNGT 5 cut(s) 643, 1003, 1033, 1048, 1513
TaiI ACGT 4 cut(s) 156, 541, 766, 2117
TaqI TCGA 5 cut(s) 864, 1054, 1264, 1945, 2245
TasI AATT 8 cut(s) 160, 261, 361, 390, 441, 1094, 1487, 2037
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 2 cut(s) 473, 1982
TscAI CASTG 3 cut(s) 646, 1162, 1734
TseFI GTSAC 2 cut(s) 539, 1027
Tsp45I GTSAC 2 cut(s) 539, 1027
TspDTI ATGAA 4 cut(s) 507, 1296, 1515, 1818
TspGWI ACGGA 2 cut(s) 865, 1257
TspRI CASTG 3 cut(s) 646, 1162, 1734
Vha464I CTTAAG 1 cut(s) 1832
VpaK11BI GGWCC 6 cut(s) 122, 503, 649, 930, 986, 1888
VspI ATTAAT 1 cut(s) 246
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 2 cut(s) 160, 361
XceI RCATGY 1 cut(s) 1427
XcmI CCANNNNNNNNNTGG 2 cut(s) 659, 1456
XhoI CTCGAG 2 cut(s) 1944, 2244
XmaJI CCTAGG 1 cut(s) 1229
XmnI GAANNNNTTC 1 cut(s) 804
XspI CTAG 8 cut(s) 374, 582, 1041, 1191, 1230, 2079, 2166, 2323
Zsp2I ATGCAT 2 cut(s) 1186, 2257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.