RchiOBHm_Chr5g0075241

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000923
Physical Location & Seq
Reverse (-)
81048978 .. 81052923
3946 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34997

Sequence Viewer

Length: 2322 bp
ATGGTGCTTTCATCTCTACCTCCATTACTATTATTATCAGCTCTTTTGTTCTCTCTGTTGCAGACACCTACCCTTTCCATTAAAAAGCCATATATAGTGTACTTAGGAGCACATTCTCATGGTCCAAGCCCATCATCTGTTGATCTCGATTTTGTCAGAAATTTTCACTACAATTTTCTGGGGTCATTCTTGAGAAGCAATAAAAGTGCCAAAGATGTGATGTTCTACTCCTATACTAGACACATTAATGGTTTTGCTGCAATTCTTGAAGAGGAAGAAGCTGCTCAGATTGCTAAGGATCCAAATGTGATATCAGTCTTCCTAAACAAGGGAAGAAAGCTACAAACTACCCGGTCATGGAATTTTCTTGGATTAGAGAGAAATGGACTTATTCCTTCAGACTCCATTTGGAAGAAAGCAAGGTTTGGCGAAGATACAATAATTGCAAACATAGATACTGGTGTTTGGCCAGAATCCAAAAGCTTTAGTGATGAAGGGCTGGGACCTGTCCCTTCCAAGTGGCGTGGAACTTGTCAACATGACACAAAGCAAGTTCATTGCAACAGAAAGCTGATTGGAACTAGGTTCTTTAATAATGGTCTTGCCATGTATGCCGGCCCTCTCAACTCGTCGTATTCCACTGTTCGCGACTACGATGGTCATGGATCACATACCCTATCCACGGCTGCGGGTGGCTTTGTTCCTGGAGTCAGTGTTTTTGGCAATGGCAATGGAACCACCAAGGGTGGCTCTCCTCAAGCCCGTGTTGCTGCATATAAGGTATGCTGGCCACCATTTGAAGGTGTTGGGTGCTTCGAGGCAGACATCTTAGCTGCCTTTGATGCTGCAATGAGTGACGGTGTCGATGTAATCTCTGTGTCTCTTGGTGGAGGAACAGAGGAGTTTTTCAACAATGCAATTTCAATAGGATCCTTCCATGCAGTTAAAAATGGCATTGTTGTGGTTAGTGCAGCTGGAAACTCTGGACCAAATCCAGGGACAGTATTAAATTTGTCGCCATGGTTAGTAACAGTTGGTGCTAGCACCATTGATCTAGAGTTCACAAGCTATGTTGTCCTCGGAAACAAAAAGCAATTGAAGGGAGCAAGCCTTTCAGCTAAAGGCTTACCATTTGAAGTGTTTTACCCGTTGATCAGTGCTGCAGATGCGAGAGATGCTGATGCATCCACTGGAAAAGCTGAAATTTGCAATGTTGGAGCTCTCGATCCTAGGAAAGTAAAAGGAAAGATTTTAGTGTGCGTTCGAGAATACAACGATAATGAAAGAACCGAGAAGAGCATGCAGGCTGATATTGCAGGTGCTGTAGGAATGATCTTGGTTAATGATAAGCAAAGTGGAAACGATGTTGTAGCCGATCCTCATGTGCTCCTTGTTTCACATCTCAATTACACCGACGGCAAACATGTCTTTGATTACATTAAATCTACCAAAACTCCTGTGGCTTACCTTACTCCAGTAAAGACTGAATTGGGAACAAAGCCAGCTCCGCTTGTAGCTCCATTTTCATCAAGGGGGCCTAATCTTCTGGAGCAGGCAATCTTTAAGCCTGATATCATTGCACCAGGGGTGAGTATAATTGCTGCTTATACTGAAGCAACAGGGCCAACTTATCAATTATCTGATACTCGCCGGGTTTCTTTTAATGTTCAAACCGGCACTTCAATGGCATGCCCTCATGTATCCGGAATTGCAGGGCTTCTGAGGAAACTCCATCCAGATTGGAGCCCAGCAGCTATTAAATCTGCAATCATGACAACTGCTACAACACAAGATGACAGCAATGAACCGATGCTTGACTCGTCTTATTTGAAGGCAACACCATTTGCTTATGGTGCAGGACACATTCAACCAAACAACGCAATGGACCCGGGGTTAATTTATAACCTAACAACACTTGATTACTTGAATTTCTTATGTGCTCGAGGATACAATGAAACAATGATCAAATCATTCTCCAATCCACCTTTCAAATGTCCCAAGTCTTTCAGTCTAGGAGACTTCAACTACCCAGCAATTGCAATTCCTAATATCGGTGCAGAGTTAGTGACCGTTACTAGAAGAAAAGTTACAAATGTCGGATCACCTGGCACGTACAAAGTGTGCCTCAAGGCACCACCAGAAGTAATGGTTTTGGTTAAACCTAGAAGCTTGAAATTTGAGAGTATTGGTGAAGTGAAAAAGTTCAAAGTTATATTCAAAGCTAAGGTTAAGGGTAAGCCTCGAGGGTATGCATTTGGAGAGTTGATGTGGTCAGATGGCAATCACCATGTAAAGATTCCTCTTGCAGTGAAGCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

773

Amino Acids

83.39

Weight (kDa)

8.95

Isoelectric Point (pI)

28.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 115 2.6e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 607 6e-40 Subtilase family
PA PF02225 382 - 474 4.1e-10 PA domain
fn3_6 PF17766 673 - 770 1.4e-23 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1902
AarI CACCTGC 1 cut(s) 1307
AbsI CCTCGAGG 1 cut(s) 2241
Acc36I ACCTGC 1 cut(s) 1307
AccB1I GGYRCC 1 cut(s) 2131
AccII CGCG 1 cut(s) 648
AccIII TCCGGA 1 cut(s) 1703
AciI CCGC 2 cut(s) 689, 1508
AclWI GGATC 8 cut(s) 293, 306, 673, 924, 937, 1220, 1370, 2107
AcoI YGGCCR 2 cut(s) 467, 788
AcsI RAATTY 6 cut(s) 160, 361, 1009, 1203, 1927, 2174
AcuI CTGAAG 2 cut(s) 381, 1632
AfaI GTAC 2 cut(s) 101, 2114
AfiI CCNNNNNNNGG 6 cut(s) 328, 357, 682, 800, 995, 2051
AflIII ACRYGT 1 cut(s) 1423
AjnI CCWGG 4 cut(s) 703, 994, 1582, 2104
AjuI GAANNNNNNNTTGG 2 cut(s) 1970, 2002
Alw21I GWGCWC 4 cut(s) 112, 1222, 1389, 1942
Alw26I GTCTC 2 cut(s) 885, 2010
AlwI GGATC 8 cut(s) 293, 306, 673, 924, 937, 1220, 1370, 2107
AlwNI CAGNNNCTG 1 cut(s) 1322
Ama87I CYCGRG 3 cut(s) 1888, 1941, 2241
Aor13HI TCCGGA 1 cut(s) 1703
AoxI GGCC 5 cut(s) 467, 616, 788, 1535, 1622
ApoI RAATTY 6 cut(s) 160, 361, 1009, 1203, 1927, 2174
AseI ATTAAT 1 cut(s) 246
Asp700I GAANNNNTTC 1 cut(s) 2201
AspA2I CCTAGG 1 cut(s) 1229
AspS9I GGNCC 7 cut(s) 122, 503, 617, 986, 1535, 1622, 1885
AsuC2I CCSGG 4 cut(s) 352, 1652, 1889, 1890
AsuHPI GGTGA 4 cut(s) 1600, 2094, 2201, 2276
AsuNHI GCTAGC 1 cut(s) 1040
AvaI CYCGRG 3 cut(s) 1888, 1941, 2241
AvaII GGWCC 4 cut(s) 122, 503, 986, 1885
AvrII CCTAGG 1 cut(s) 1229
BaeI ACNNNNGTAYC 2 cut(s) 447, 480
BalI TGGCCA 2 cut(s) 469, 790
BamHI GGATCC 2 cut(s) 298, 929
BanI GGYRCC 1 cut(s) 2131
BanII GRGCYC 2 cut(s) 1222, 1748
BarI GAAGNNNNNNTAC 2 cut(s) 2071, 2103
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 4 cut(s) 112, 1222, 1389, 1942
BccI CCATC 4 cut(s) 139, 650, 1740, 2270
BceAI ACGGC 2 cut(s) 699, 1432
BciT130I CCWGG 4 cut(s) 705, 996, 1584, 2106
BciVI GTATCC 2 cut(s) 1711, 1940
BclI TGATCA 2 cut(s) 1152, 1962
BcnI CCSGG 4 cut(s) 352, 1652, 1889, 1890
BcoDI GTCTC 2 cut(s) 885, 2010
BfaI CTAG 9 cut(s) 237, 582, 1041, 1055, 1230, 2012, 2076, 2163, 2320
BfmI CTRYAG 2 cut(s) 1161, 1323
BfuAI ACCTGC 1 cut(s) 1307
BfuI GTATCC 2 cut(s) 1711, 1940
BlnI CCTAGG 1 cut(s) 1229
Bme1390I CCNGG 8 cut(s) 352, 705, 996, 1584, 1652, 1889, 1890, 2106
Bme18I GGWCC 4 cut(s) 122, 503, 986, 1885
BmeT110I CYCGRG 3 cut(s) 1888, 1941, 2241
BmgT120I GGNCC 7 cut(s) 122, 503, 617, 986, 1535, 1622, 1885
BmiI GGNNCC 8 cut(s) 300, 504, 736, 931, 1536, 1745, 1887, 2133
BmrFI CCNGG 8 cut(s) 352, 705, 996, 1584, 1652, 1889, 1890, 2106
BmsI GCATC 6 cut(s) 832, 1156, 1165, 1171, 1193, 1800
BmtI GCTAGC 1 cut(s) 1044
BpiI GAAGAC 1 cut(s) 310
BpmI CTGGAG 3 cut(s) 726, 1458, 1568
Bpu10I CCTNAGC 2 cut(s) 294, 2223
BpuEI CTTGAG 3 cut(s) 211, 741, 2111
BpuMI CCSGG 4 cut(s) 352, 1652, 1889, 1890
BsaAI YACGTR 1 cut(s) 2112
BsaBI GATNNNNATC 1 cut(s) 2280
BsaJI CCNNGG 9 cut(s) 681, 741, 995, 1019, 1078, 1229, 1583, 1888, 1889
BsaWI WCCGGW 1 cut(s) 1703
BsaXI ACNNNNNCTCC 4 cut(s) 699, 729, 1438, 1468
Bsc4I CCNNNNNNNGG 6 cut(s) 328, 357, 682, 800, 995, 2051
Bse118I RCCGGY 2 cut(s) 614, 1673
Bse1I ACTGG 3 cut(s) 463, 1195, 1475
Bse3DI GCAATG 8 cut(s) 556, 730, 736, 855, 1216, 1575, 1807, 1887
Bse8I GATNNNNATC 1 cut(s) 2280
BseAI TCCGGA 1 cut(s) 1703
BseBI CCWGG 4 cut(s) 705, 996, 1584, 2106
BseDI CCNNGG 9 cut(s) 681, 741, 995, 1019, 1078, 1229, 1583, 1888, 1889
BseGI GGATG 2 cut(s) 1184, 1732
BseJI GATNNNNATC 1 cut(s) 2280
BseLI CCNNNNNNNGG 6 cut(s) 328, 357, 682, 800, 995, 2051
BseMI GCAATG 8 cut(s) 556, 730, 736, 855, 1216, 1575, 1807, 1887
BseMII CTCAG 2 cut(s) 299, 1712
BseNI ACTGG 3 cut(s) 463, 1195, 1475
BseRI GAGGAG 2 cut(s) 744, 914
BseYI CCCAGC 3 cut(s) 499, 1747, 2029
BsgI GTGCAG 3 cut(s) 990, 1875, 2076
Bsh1236I CGCG 1 cut(s) 648
BshFI GGCC 5 cut(s) 469, 618, 790, 1537, 1624
BshNI GGYRCC 1 cut(s) 2131
BsiHKAI GWGCWC 4 cut(s) 112, 1222, 1389, 1942
BsiHKCI CYCGRG 3 cut(s) 1888, 1941, 2241
BsiSI CCGG 6 cut(s) 352, 615, 1651, 1674, 1704, 1889
BslFI GGGAC 4 cut(s) 494, 516, 1012, 1980
BslI CCNNNNNNNGG 6 cut(s) 328, 357, 682, 800, 995, 2051
BsmAI GTCTC 2 cut(s) 885, 2010
BsmFI GGGAC 4 cut(s) 494, 516, 1012, 1980
BsnI GGCC 5 cut(s) 469, 618, 790, 1537, 1624
BsoBI CYCGRG 3 cut(s) 1888, 1941, 2241
Bsp1286I GDGCHC 5 cut(s) 112, 1222, 1389, 1748, 1942
Bsp13I TCCGGA 1 cut(s) 1703
Bsp19I CCATGG 1 cut(s) 1019
Bsp68I TCGCGA 1 cut(s) 648
BspACI CCGC 2 cut(s) 689, 1508
BspANI GGCC 5 cut(s) 469, 618, 790, 1537, 1624
BspCNI CTCAG 2 cut(s) 298, 1713
BspEI TCCGGA 1 cut(s) 1703
BspFNI CGCG 1 cut(s) 648
BspHI TCATGA 1 cut(s) 1770
BspLI GGNNCC 8 cut(s) 300, 504, 736, 931, 1536, 1745, 1887, 2133
BspMAI CTGCAG 1 cut(s) 1165
BspMI ACCTGC 1 cut(s) 1307
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 8 cut(s) 293, 306, 673, 924, 937, 1220, 1370, 2107
BspQI GCTCTTC 1 cut(s) 1289
BspT107I GGYRCC 1 cut(s) 2131
BsrDI GCAATG 8 cut(s) 556, 730, 736, 855, 1216, 1575, 1807, 1887
BsrFI RCCGGY 2 cut(s) 614, 1673
BsrI ACTGG 3 cut(s) 463, 1195, 1475
BssAI RCCGGY 2 cut(s) 614, 1673
BssECI CCNNGG 9 cut(s) 681, 741, 995, 1019, 1078, 1229, 1583, 1888, 1889
BssT1I CCWWGG 3 cut(s) 741, 1019, 1229
Bst2UI CCWGG 4 cut(s) 705, 996, 1584, 2106
Bst4CI ACNGT 5 cut(s) 643, 860, 1003, 1033, 2071
Bst6I CTCTTC 2 cut(s) 264, 1289
BstBAI YACGTR 1 cut(s) 2112
BstC8I GCNNGC 9 cut(s) 616, 788, 1042, 1108, 1301, 1305, 1503, 1554, 1690
BstDEI CTNAG 6 cut(s) 103, 285, 294, 829, 1721, 2223
BstDSI CCRYGG 2 cut(s) 681, 1019
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 2 cut(s) 1184, 1732
BstFNI CGCG 1 cut(s) 648
BstMAI GTCTC 2 cut(s) 885, 2010
BstMWI GCNNNNNNNGC 9 cut(s) 290, 611, 767, 842, 1166, 1175, 1313, 1507, 1853
BstNI CCWGG 4 cut(s) 705, 996, 1584, 2106
BstNSI RCATGY 3 cut(s) 1303, 1427, 1692
BstSCI CCNGG 8 cut(s) 350, 703, 994, 1582, 1650, 1887, 1888, 2104
BstSFI CTRYAG 2 cut(s) 1161, 1323
BstUI CGCG 1 cut(s) 648
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 2 cut(s) 298, 929
BstYI RGATCY 2 cut(s) 298, 929
BsuI GTATCC 2 cut(s) 1711, 1940
BsuRI GGCC 5 cut(s) 469, 618, 790, 1537, 1624
BtgI CCRYGG 2 cut(s) 681, 1019
BtsCI GGATG 2 cut(s) 1184, 1732
BtsI GCAGTG 1 cut(s) 2313
BtsIMutI CAGTG 5 cut(s) 639, 718, 1162, 1188, 2313
BtuMI TCGCGA 1 cut(s) 648
BveI ACCTGC 1 cut(s) 1307
Cac8I GCNNGC 9 cut(s) 616, 788, 1042, 1108, 1301, 1305, 1503, 1554, 1690
CaiI CAGNNNCTG 1 cut(s) 1322
CciI TCATGA 1 cut(s) 1770
Cfr10I RCCGGY 2 cut(s) 614, 1673
Cfr13I GGNCC 7 cut(s) 122, 503, 617, 986, 1535, 1622, 1885
Cfr9I CCCGGG 1 cut(s) 1888
Csp6I GTAC 2 cut(s) 100, 2113
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviQI GTAC 2 cut(s) 100, 2113
DdeI CTNAG 6 cut(s) 103, 285, 294, 829, 1721, 2223
EaeI YGGCCR 2 cut(s) 467, 788
Eam1104I CTCTTC 2 cut(s) 264, 1289
EarI CTCTTC 2 cut(s) 264, 1289
Ecl136II GAGCTC 1 cut(s) 1220
Eco130I CCWWGG 3 cut(s) 741, 1019, 1229
Eco24I GRGCYC 2 cut(s) 1222, 1748
Eco32I GATATC 2 cut(s) 312, 1573
Eco47I GGWCC 4 cut(s) 122, 503, 986, 1885
Eco53kI GAGCTC 1 cut(s) 1220
Eco57I CTGAAG 2 cut(s) 381, 1632
Eco88I CYCGRG 3 cut(s) 1888, 1941, 2241
EcoICRI GAGCTC 1 cut(s) 1220
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoO109I RGGNCCY 2 cut(s) 503, 1535
EcoRII CCWGG 4 cut(s) 703, 994, 1582, 2104
EcoRV GATATC 2 cut(s) 312, 1573
EcoT14I CCWWGG 3 cut(s) 741, 1019, 1229
EcoT22I ATGCAT 2 cut(s) 1186, 2254
EcoT38I GRGCYC 2 cut(s) 1222, 1748
ErhI CCWWGG 3 cut(s) 741, 1019, 1229
FaqI GGGAC 4 cut(s) 494, 516, 1012, 1980
FauI CCCGC 1 cut(s) 682
FbaI TGATCA 2 cut(s) 1152, 1962
FokI GGATG 2 cut(s) 1171, 1719
FriOI GRGCYC 2 cut(s) 1222, 1748
FspBI CTAG 9 cut(s) 237, 582, 1041, 1055, 1230, 2012, 2076, 2163, 2320
GsaI CCCAGC 3 cut(s) 503, 1751, 2033
GsuI CTGGAG 3 cut(s) 726, 1458, 1568
HaeIII GGCC 5 cut(s) 469, 618, 790, 1537, 1624
HapII CCGG 6 cut(s) 352, 615, 1651, 1674, 1704, 1889
HincII GTYRAC 1 cut(s) 536
HindII GTYRAC 1 cut(s) 536
HindIII AAGCTT 2 cut(s) 481, 2167
HinfI GANTC 5 cut(s) 401, 473, 708, 1817, 2296
HpaII CCGG 6 cut(s) 352, 615, 1651, 1674, 1704, 1889
HphI GGTGA 4 cut(s) 1600, 2094, 2201, 2276
Hpy166II GTNNAC 3 cut(s) 100, 536, 1062
Hpy188I TCNGA 8 cut(s) 158, 288, 400, 1082, 1642, 1722, 2099, 2275
Hpy8I GTNNAC 3 cut(s) 100, 536, 1062
Hpy99I CGWCG 2 cut(s) 634, 1418
HpyAV CCTTC 7 cut(s) 405, 488, 522, 794, 943, 1093, 1825
HpyCH4III ACNGT 5 cut(s) 643, 860, 1003, 1033, 2071
HpyCH4IV ACGT 1 cut(s) 2111
HpyF10VI GCNNNNNNNGC 9 cut(s) 290, 611, 767, 842, 1166, 1175, 1313, 1507, 1853
HpyF3I CTNAG 6 cut(s) 103, 285, 294, 829, 1721, 2223
HpySE526I ACGT 1 cut(s) 2111
Kpn2I TCCGGA 1 cut(s) 1703
KroI GCCGGC 1 cut(s) 614
KroNI GCCGGC 1 cut(s) 616
Ksp22I TGATCA 2 cut(s) 1152, 1962
LguI GCTCTTC 1 cut(s) 1289
LmnI GCTCC 8 cut(s) 107, 1103, 1217, 1392, 1510, 1522, 1549, 1743
LweI GCATC 6 cut(s) 832, 1156, 1165, 1171, 1193, 1800
MaeI CTAG 9 cut(s) 237, 582, 1041, 1055, 1230, 2012, 2076, 2163, 2320
MaeII ACGT 1 cut(s) 2111
MaeIII GTNAC 5 cut(s) 854, 1027, 2065, 2071, 2086
MboII GAAGA 9 cut(s) 281, 287, 310, 345, 424, 443, 1306, 1535, 2091
MfeI CAATTG 2 cut(s) 1094, 2034
MflI RGATCY 2 cut(s) 298, 929
MhlI GDGCHC 5 cut(s) 112, 1222, 1389, 1748, 1942
MlsI TGGCCA 2 cut(s) 469, 790
MluNI TGGCCA 2 cut(s) 469, 790
MlyI GAGTC 3 cut(s) 395, 717, 1811
MmeI TCCRAC 2 cut(s) 1195, 2077
Mox20I TGGCCA 2 cut(s) 469, 790
Mph1103I ATGCAT 2 cut(s) 1186, 2254
MroI TCCGGA 1 cut(s) 1703
MroNI GCCGGC 1 cut(s) 614
MroXI GAANNNNTTC 1 cut(s) 2201
MscI TGGCCA 2 cut(s) 469, 790
MslI CAYNNNNRTG 4 cut(s) 117, 246, 959, 1682
Msp20I TGGCCA 2 cut(s) 469, 790
MspA1I CMGCKG 1 cut(s) 974
MspI CCGG 6 cut(s) 352, 615, 1651, 1674, 1704, 1889
MspR9I CCNGG 8 cut(s) 352, 705, 996, 1584, 1652, 1889, 1890, 2106
MunI CAATTG 2 cut(s) 1094, 2034
MvaI CCWGG 4 cut(s) 705, 996, 1584, 2106
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 9 cut(s) 290, 611, 767, 842, 1166, 1175, 1313, 1507, 1853
NaeI GCCGGC 1 cut(s) 616
NciI CCSGG 4 cut(s) 352, 1652, 1889, 1890
NcoI CCATGG 1 cut(s) 1019
NgoMIV GCCGGC 1 cut(s) 614
NheI GCTAGC 1 cut(s) 1040
NlaIV GGNNCC 8 cut(s) 300, 504, 736, 931, 1536, 1745, 1887, 2133
NmuCI GTSAC 2 cut(s) 854, 2065
NruI TCGCGA 1 cut(s) 648
NsiI ATGCAT 2 cut(s) 1186, 2254
NspI RCATGY 3 cut(s) 1303, 1427, 1692
PaeI GCATGC 2 cut(s) 1303, 1692
PaeR7I CTCGAG 2 cut(s) 1941, 2241
PagI TCATGA 1 cut(s) 1770
PaqCI CACCTGC 1 cut(s) 1307
PciI ACATGT 1 cut(s) 1423
PciSI GCTCTTC 1 cut(s) 1289
PdiI GCCGGC 1 cut(s) 616
PdmI GAANNNNTTC 1 cut(s) 2201
PfeI GAWTC 2 cut(s) 473, 2296
PflFI GACNNNGTC 1 cut(s) 860
PfoI TCCNGGA 1 cut(s) 703
PleI GAGTC 3 cut(s) 395, 716, 1811
PpsI GAGTC 3 cut(s) 395, 716, 1811
Ppu21I YACGTR 1 cut(s) 2112
PpuMI RGGWCCY 1 cut(s) 503
PscI ACATGT 1 cut(s) 1423
PshBI ATTAAT 1 cut(s) 246
PsiI TTATAA 1 cut(s) 1902
Psp124BI GAGCTC 1 cut(s) 1222
Psp5II RGGWCCY 1 cut(s) 503
Psp6I CCWGG 4 cut(s) 703, 994, 1582, 2104
PspFI CCCAGC 3 cut(s) 499, 1747, 2029
PspGI CCWGG 4 cut(s) 703, 994, 1582, 2104
PspN4I GGNNCC 8 cut(s) 300, 504, 736, 931, 1536, 1745, 1887, 2133
PspPI GGNCC 7 cut(s) 122, 503, 617, 986, 1535, 1622, 1885
PspPPI RGGWCCY 1 cut(s) 503
PspXI VCTCGAGB 2 cut(s) 1941, 2241
PstI CTGCAG 1 cut(s) 1165
PstNI CAGNNNCTG 1 cut(s) 1322
PsuI RGATCY 2 cut(s) 298, 929
PsyI GACNNNGTC 1 cut(s) 860
PvuII CAGCTG 1 cut(s) 974
RruI TCGCGA 1 cut(s) 648
RsaI GTAC 2 cut(s) 101, 2114
RsaNI GTAC 2 cut(s) 100, 2113
RseI CAYNNNNRTG 4 cut(s) 117, 246, 959, 1682
SacI GAGCTC 1 cut(s) 1222
SapI GCTCTTC 1 cut(s) 1289
Sau96I GGNCC 7 cut(s) 122, 503, 617, 986, 1535, 1622, 1885
SchI GAGTC 3 cut(s) 395, 717, 1811
ScrFI CCNGG 8 cut(s) 352, 705, 996, 1584, 1652, 1889, 1890, 2106
SduI GDGCHC 5 cut(s) 112, 1222, 1389, 1748, 1942
SfaNI GCATC 6 cut(s) 832, 1156, 1165, 1171, 1193, 1800
SfcI CTRYAG 2 cut(s) 1161, 1323
Sfr274I CTCGAG 2 cut(s) 1941, 2241
SinI GGWCC 4 cut(s) 122, 503, 986, 1885
SlaI CTCGAG 2 cut(s) 1941, 2241
SmaI CCCGGG 1 cut(s) 1890
SmiMI CAYNNNNRTG 4 cut(s) 117, 246, 959, 1682
SmlI CTYRAG 5 cut(s) 190, 756, 1941, 2126, 2241
SmoI CTYRAG 5 cut(s) 190, 756, 1941, 2126, 2241
SphI GCATGC 2 cut(s) 1303, 1692
SsiI CCGC 2 cut(s) 689, 1508
SspMI CTAG 9 cut(s) 237, 582, 1041, 1055, 1230, 2012, 2076, 2163, 2320
SstI GAGCTC 1 cut(s) 1222
StyD4I CCNGG 8 cut(s) 350, 703, 994, 1582, 1650, 1887, 1888, 2104
StyI CCWWGG 3 cut(s) 741, 1019, 1229
TaaI ACNGT 5 cut(s) 643, 860, 1003, 1033, 2071
TaiI ACGT 1 cut(s) 2114
TaqI TCGA 7 cut(s) 147, 816, 864, 1224, 1264, 1942, 2242
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 2 cut(s) 473, 2296
TscAI CASTG 5 cut(s) 646, 718, 1162, 1195, 2313
TseFI GTSAC 2 cut(s) 854, 2065
Tsp45I GTSAC 2 cut(s) 854, 2065
TspDTI ATGAA 6 cut(s) 507, 545, 1296, 1515, 1818, 1968
TspMI CCCGGG 1 cut(s) 1888
TspRI CASTG 5 cut(s) 646, 718, 1162, 1195, 2313
Tth111I GACNNNGTC 1 cut(s) 860
VpaK11BI GGWCC 4 cut(s) 122, 503, 986, 1885
VspI ATTAAT 1 cut(s) 246
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 6 cut(s) 160, 361, 1009, 1203, 1927, 2174
XbaI TCTAGA 1 cut(s) 1054
XceI RCATGY 3 cut(s) 1303, 1427, 1692
XcmI CCANNNNNNNNNTGG 1 cut(s) 1456
XhoI CTCGAG 2 cut(s) 1941, 2241
XmaI CCCGGG 1 cut(s) 1888
XmaJI CCTAGG 1 cut(s) 1229
XmnI GAANNNNTTC 1 cut(s) 2201
XspI CTAG 9 cut(s) 237, 582, 1041, 1055, 1230, 2012, 2076, 2163, 2320
Zsp2I ATGCAT 2 cut(s) 1186, 2254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.