MD11G1039600.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000923
Physical Location & Seq
Forward (+)
3417424 .. 3423071
5648 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1039600.v1.1.491

Sequence Viewer

Length: 2331 bp
ATGGGAAATTCAATTCTCCCTTCTTTTCTTCTCTCAGTTCTTCTCATCTCTTTGTTTCATATTCCAGCGCATGCTGCCAAAAGTTCTTACATTGTGTACTTAGGAGCAGGTCCTCATGTTCTGGACCCTTTGTCCGCCGATCTCGATAGCGTGACAAATTCTCATTACAATCTTCTTGGAACAGTTTTGGGAAGCAATGAAAGGGCACAGGAATCAATATTTTACTCATATACGAGAAATATCAATGGCTTTGCTGCAATTTTGGATGATGAAGAGGCTGCTCAGATTGCAAAGGATCCAAATGTTGTGTCTGTTTTTCCAAACAGAGGAAGAAAACTACAGACAACTCGTTCATGGGATTTTCTTCGATTGGAAGAAAACGGCGAAATCCGGGCCGGCTCTATTTGGAAGAAGGCACGATTAGGTGCAAATACAATTATCGGAAACCTTGATACTGGTGTTTGGCCGGAATCAAAGAGCTTTAGCGATGAAGGGATGGGACCAATCCCATCTAAATGGCGTGGAATCTGTCAGCTGGACACCAAAGATGGTTCTCGTTGCAATAGGAAGCTGATCGGAGCAAGGTACTTCAGCAAAGGATACGCCGTGTATGCTTCCACGGTTAACTCCTCTGCAGCCAAGTCCATCCAGCCCAACGCTCGTGACCATGCCGGTCATGGCTCACACACCCTTTCAACGGCAGGTGGTAATTTTGTTCCTAGAGCAAGTGTTTTCGGTAATGGCAATGGCACTGCAAAGGGTGGATCCCCAAAAGCTCGCGTGGCTGCGTATAAAGTATGCTGGCCTCCAATCGATGGCAACGAGTGCTTCGATGCAGACATCTTGGCCGCATTTGAAGCTGCAATTAATGATGGTGTTGATGTGCTCTCAGTGTCTCTTGGTGGTGAAGCTGCGGAGTTTTTAAGTGATGGGATTGCAATAGGGTCCTTCCATGCTGTTAAGAAGGGGATTACCGTGGTGAGCTCAGCTGGGAATTCAGGTCCTACTCCTGGGTCAGTGTCGAATGTGTCACCGTGGATGCTCACAGTTGGAGCTAGCACGATTGATCGCGAGTTCTCCAGTTATGTTACTCTTGGCAACAAGAAGCATCTCAAGGGAGCAAGTCTTTCCTCTACAGCTTTGCCAGCTAAAAAGTTCTACCCATTGATAAGTGCATGCGATGCTAAAGCTGCTAATGCGTCTGGTTCTGAAGCCCATCTCTGCAAACCTGGAACCCTCGATAAAAAGAAGGCAAAGGGGAAAATTTTGGTTTGCGTTCGAGGGCAAAATCCAAGAGCCAACAAGGGTCAACAAGCTATTCTTGCAGGCGCTGTTGGAATGATTTTGGTTAATGATAAGCTAAGCGGGAATGAAATCATAGCTGATCCTCACTTGCTTCCAGCTTCACATGTTAATTATTCTGACGGAAAATCTGTCTTTGCCTACATCAAGTCGACCAAGAATCCTATGGCTTACATTAATCGTGTAAAGACAGAACAAGGAACAAAACCAGCTCCATTTATGGCTTCATTCTCTTCAAGGGGACCCAACGCCATTGAGCAGTCAATCCTTAAGCCTGATATCACAGCGCCAGGAGTGAGTATAATTGCTGCTTATACTCAAGCAACCGGGCCAACTGGTCAGAAGTTCGATAACCGGCGTGTATCTTTCAATACACAATCCGGAACTTCCATGTCGTGCCCTCATGTATCTGGAATTGTCGGTCTTTTGAAAACCCTTCACCCGACATGGAGCCCTGCAGCTATTAAATCTGCAATCATGACCACCGCAAGTAAACGAGATAACAGCAAGGGCACAATGCTGGACTCATCCAAGGCCAGAGCAACACCGTTTGCTTATGGTGCAGGACATGTTCAACCAAACCGTGCGATGGACCCTGGACTTGTTTATGACCTAACTACTGATGATTACTTAAACTTCTTATGCGCTCGTGGCTACAATGCAACGCTGCTCAAAGTGTTTTCTAAAGAGCCACACAAATGTTCCAAGGCATATAGTCTTTTTGATTTTAACTATCCTTCTATAACAGTTCCCAATCTCCGTGACACGCCAGTGACTGTGACTAGAAGGGTTAAGAACGTCGGCTCTCCAGGCACATATGTAGTTCGTATTAAGGAACCGGTGGGAGTTTCTGTTACAGTTAAGCCTAGTACCTTGCAATTCAAGAACAGTGGCGAAGAGAAGAAGTTCAAGGTTGTTCTGAAGCCTAAGGTTCAAGGGCCTGAAGACTACGTATTTGGAGAGTTAAATTGGACGGATGGGAAGCACAATGTCAGGAGTCCTATTGTTGTCATGCACTATCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

777

Amino Acids

82.95

Weight (kDa)

9.5

Isoelectric Point (pI)

29.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 29 - 114 7e-18 Peptidase inhibitor I9
MORF_dom PF21864 52 - 109 5.4e-07 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 142 - 609 8.5e-40 Subtilase family
PA PF02225 387 - 478 3.5e-13 PA domain
fn3_6 PF17766 676 - 771 6.3e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 692
Acc36I ACCTGC 2 cut(s) 98, 692
AccB7I CCANNNNNTGG 1 cut(s) 815
AccI GTMKAC 1 cut(s) 1454
AccII CGCG 2 cut(s) 780, 1071
AccIII TCCGGA 1 cut(s) 1682
AciI CCGC 5 cut(s) 135, 849, 914, 1365, 1788
AclWI GGATC 5 cut(s) 290, 303, 759, 772, 1379
AcoI YGGCCR 2 cut(s) 464, 846
AcsI RAATTY 4 cut(s) 7, 157, 994, 1263
AcuI CTGAAG 4 cut(s) 574, 1230, 2243, 2265
AfaI GTAC 3 cut(s) 98, 587, 2173
AfiI CCNNNNNNNGG 5 cut(s) 326, 697, 815, 1010, 1891
AflII CTTAAG 1 cut(s) 1571
AflIII ACRYGT 2 cut(s) 1408, 1870
AgeI ACCGGT 1 cut(s) 2140
AhdI GACNNNNNGTC 1 cut(s) 130
AjnI CCWGG 5 cut(s) 1009, 1228, 1591, 1897, 2110
AleI CACNNNNGTG 1 cut(s) 2072
Alw21I GWGCWC 2 cut(s) 888, 986
Alw26I GTCTC 1 cut(s) 900
AlwI GGATC 5 cut(s) 290, 303, 759, 772, 1379
AlwNI CAGNNNCTG 2 cut(s) 1331, 2078
Aor13HI TCCGGA 1 cut(s) 1682
AoxI GGCC 7 cut(s) 393, 464, 803, 846, 1631, 1836, 2240
ApoI RAATTY 4 cut(s) 7, 157, 994, 1263
ArsI GACNNNNNNTTYG 4 cut(s) 293, 325, 2240, 2272
AseI ATTAAT 2 cut(s) 867, 1479
AsiGI ACCGGT 1 cut(s) 2140
Asp700I GAANNNNTTC 1 cut(s) 2207
AspLEI GCGC 4 cut(s) 70, 1331, 1591, 1949
AsuC2I CCSGG 2 cut(s) 392, 1630
AsuHPI GGTGA 4 cut(s) 917, 991, 1023, 1733
AsuNHI GCTAGC 1 cut(s) 1055
AvaII GGWCC 7 cut(s) 110, 124, 500, 945, 1001, 1544, 1894
AxyI CCTNAGG 1 cut(s) 2229
BaeGI GKGCMC 3 cut(s) 208, 1703, 1817
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BamHI GGATCC 2 cut(s) 295, 764
BanII GRGCYC 2 cut(s) 986, 1757
BauI CACGAG 2 cut(s) 660, 1950
BbsI GAAGAC 1 cut(s) 2253
Bbv12I GWGCWC 2 cut(s) 888, 986
BceAI ACGGC 3 cut(s) 397, 590, 714
BciT130I CCWGG 5 cut(s) 1011, 1230, 1593, 1899, 2112
BciVI GTATCC 1 cut(s) 593
BcnI CCSGG 2 cut(s) 392, 1630
BcoDI GTCTC 1 cut(s) 900
BfaI CTAG 4 cut(s) 720, 1056, 2085, 2169
BfmI CTRYAG 4 cut(s) 338, 633, 1134, 1758
BfoI RGCGCY 2 cut(s) 1332, 1592
BfrI CTTAAG 1 cut(s) 1571
BfuAI ACCTGC 2 cut(s) 98, 692
BfuI GTATCC 1 cut(s) 593
BlpI GCTNAGC 2 cut(s) 985, 1361
Bme1390I CCNGG 7 cut(s) 392, 1011, 1230, 1593, 1630, 1899, 2112
Bme18I GGWCC 7 cut(s) 110, 124, 500, 945, 1001, 1544, 1894
BmeRI GACNNNNNGTC 1 cut(s) 130
BmrFI CCNGG 7 cut(s) 392, 1011, 1230, 1593, 1630, 1899, 2112
BmsI GCATC 4 cut(s) 823, 1029, 1117, 1171
BmtI GCTAGC 1 cut(s) 1059
BpiI GAAGAC 1 cut(s) 2253
BpmI CTGGAG 2 cut(s) 1063, 2094
Bpu1102I GCTNAGC 2 cut(s) 985, 1361
BpuEI CTTGAG 2 cut(s) 1097, 1605
BpuMI CCSGG 2 cut(s) 392, 1630
Bsa29I ATCGAT 1 cut(s) 813
BsaAI YACGTR 1 cut(s) 2254
BsaJI CCNNGG 7 cut(s) 618, 975, 1010, 1034, 1833, 1897, 2007
BsaWI WCCGGW 2 cut(s) 1682, 2140
BsaXI ACNNNNNCTCC 2 cut(s) 2139, 2169
Bsc4I CCNNNNNNNGG 5 cut(s) 326, 697, 815, 1010, 1891
Bse118I RCCGGY 4 cut(s) 395, 671, 1656, 2140
Bse1I ACTGG 4 cut(s) 460, 1080, 1642, 2072
Bse21I CCTNAGG 1 cut(s) 2229
Bse3DI GCAATG 2 cut(s) 202, 751
BseAI TCCGGA 1 cut(s) 1682
BseBI CCWGG 5 cut(s) 1011, 1230, 1593, 1899, 2112
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 7 cut(s) 618, 975, 1010, 1034, 1833, 1897, 2007
BseGI GGATG 6 cut(s) 271, 501, 645, 1044, 1829, 2284
BseLI CCNNNNNNNGG 5 cut(s) 326, 697, 815, 1010, 1891
BseMI GCAATG 2 cut(s) 202, 751
BseMII CTCAG 4 cut(s) 48, 296, 903, 999
BseNI ACTGG 4 cut(s) 460, 1080, 1642, 2072
BseRI GAGGAG 1 cut(s) 619
BseSI GKGCMC 3 cut(s) 208, 1703, 1817
BseYI CCCAGC 1 cut(s) 989
BsgI GTGCAG 1 cut(s) 1884
Bsh1236I CGCG 2 cut(s) 780, 1071
BshFI GGCC 7 cut(s) 395, 466, 805, 848, 1633, 1838, 2242
BshTI ACCGGT 1 cut(s) 2140
BshVI ATCGAT 1 cut(s) 813
BsiHKAI GWGCWC 2 cut(s) 888, 986
BsiSI CCGG 8 cut(s) 391, 396, 467, 672, 1629, 1657, 1683, 2141
BslFI GGGAC 2 cut(s) 513, 1557
BslI CCNNNNNNNGG 5 cut(s) 326, 697, 815, 1010, 1891
BsmAI GTCTC 1 cut(s) 900
BsmFI GGGAC 2 cut(s) 513, 1557
BsnI GGCC 7 cut(s) 395, 466, 805, 848, 1633, 1838, 2242
Bsp1286I GDGCHC 6 cut(s) 208, 888, 986, 1703, 1757, 1817
Bsp13I TCCGGA 1 cut(s) 1682
Bsp143I GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
Bsp1720I GCTNAGC 2 cut(s) 985, 1361
Bsp68I TCGCGA 1 cut(s) 1071
BspACI CCGC 5 cut(s) 135, 849, 914, 1365, 1788
BspANI GGCC 7 cut(s) 395, 466, 805, 848, 1633, 1838, 2242
BspCNI CTCAG 4 cut(s) 47, 295, 902, 998
BspDI ATCGAT 1 cut(s) 813
BspEI TCCGGA 1 cut(s) 1682
BspFNI CGCG 2 cut(s) 780, 1071
BspHI TCATGA 1 cut(s) 1779
BspMAI CTGCAG 2 cut(s) 637, 1762
BspMI ACCTGC 2 cut(s) 98, 692
BspOI GCTAGC 1 cut(s) 1059
BspPI GGATC 5 cut(s) 290, 303, 759, 772, 1379
BspTI CTTAAG 1 cut(s) 1571
BsrDI GCAATG 2 cut(s) 202, 751
BsrFI RCCGGY 4 cut(s) 395, 671, 1656, 2140
BsrI ACTGG 4 cut(s) 460, 1080, 1642, 2072
BssAI RCCGGY 4 cut(s) 395, 671, 1656, 2140
BssECI CCNNGG 7 cut(s) 618, 975, 1010, 1034, 1833, 1897, 2007
BssMI GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
BssSI CACGAG 2 cut(s) 660, 1950
BssT1I CCWWGG 2 cut(s) 1833, 2007
Bst2BI CACGAG 2 cut(s) 660, 1950
Bst2UI CCWGG 5 cut(s) 1011, 1230, 1593, 1899, 2112
Bst6I CTCTTC 3 cut(s) 267, 1540, 2193
BstAFI CTTAAG 1 cut(s) 1571
BstAPI GCANNNNNTGC 2 cut(s) 825, 1181
BstBAI YACGTR 1 cut(s) 2254
BstC8I GCNNGC 8 cut(s) 72, 397, 778, 803, 1057, 1146, 1177, 1327
BstDEI CTNAG 7 cut(s) 34, 100, 282, 889, 985, 1361, 2229
BstDSI CCRYGG 3 cut(s) 618, 975, 1034
BstF5I GGATG 6 cut(s) 271, 501, 645, 1044, 1829, 2284
BstFNI CGCG 2 cut(s) 780, 1071
BstH2I RGCGCY 2 cut(s) 1332, 1592
BstHHI GCGC 4 cut(s) 70, 1331, 1591, 1949
BstKTI GATC 6 cut(s) 142, 298, 576, 767, 1069, 1387
BstMAI GTCTC 1 cut(s) 900
BstMBI GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
BstNI CCWGG 5 cut(s) 1011, 1230, 1593, 1899, 2112
BstNSI RCATGY 4 cut(s) 74, 1179, 1412, 1874
BstSCI CCNGG 7 cut(s) 390, 1009, 1228, 1591, 1628, 1897, 2110
BstSFI CTRYAG 4 cut(s) 338, 633, 1134, 1758
BstSLI GKGCMC 3 cut(s) 208, 1703, 1817
BstSNI TACGTA 1 cut(s) 2254
BstUI CGCG 2 cut(s) 780, 1071
BstV2I GAAGAC 1 cut(s) 2253
BstX2I RGATCY 2 cut(s) 295, 764
BstXI CCANNNNNNTGG 1 cut(s) 516
BstYI RGATCY 2 cut(s) 295, 764
Bsu15I ATCGAT 1 cut(s) 813
Bsu36I CCTNAGG 1 cut(s) 2229
BsuI GTATCC 1 cut(s) 593
BsuRI GGCC 7 cut(s) 395, 466, 805, 848, 1633, 1838, 2242
BsuTUI ATCGAT 1 cut(s) 813
BtgI CCRYGG 3 cut(s) 618, 975, 1034
BtgZI GCGATG 3 cut(s) 501, 1194, 1904
BtsCI GGATG 6 cut(s) 271, 501, 645, 1044, 1829, 2284
BtsI GCAGTG 1 cut(s) 750
BtsIMutI CAGTG 5 cut(s) 750, 897, 1023, 2079, 2197
BtuMI TCGCGA 1 cut(s) 1071
BveI ACCTGC 2 cut(s) 98, 692
Cac8I GCNNGC 8 cut(s) 72, 397, 778, 803, 1057, 1146, 1177, 1327
CaiI CAGNNNCTG 2 cut(s) 1331, 2078
CciI TCATGA 1 cut(s) 1779
CfoI GCGC 4 cut(s) 70, 1331, 1591, 1949
Cfr10I RCCGGY 4 cut(s) 395, 671, 1656, 2140
ClaI ATCGAT 1 cut(s) 813
CseI GACGC 1 cut(s) 1188
Csp6I GTAC 3 cut(s) 97, 586, 2172
CspAI ACCGGT 1 cut(s) 2140
CspCI CAANNNNNGTGG 2 cut(s) 2173, 2208
CviQI GTAC 3 cut(s) 97, 586, 2172
DdeI CTNAG 7 cut(s) 34, 100, 282, 889, 985, 1361, 2229
DpnI GATC 6 cut(s) 141, 297, 575, 766, 1068, 1386
DpnII GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
DriI GACNNNNNGTC 1 cut(s) 130
EaeI YGGCCR 2 cut(s) 464, 846
Eam1104I CTCTTC 3 cut(s) 267, 1540, 2193
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 3 cut(s) 267, 1540, 2193
EciI GGCGGA 1 cut(s) 124
Ecl136II GAGCTC 1 cut(s) 984
Eco105I TACGTA 1 cut(s) 2254
Eco130I CCWWGG 2 cut(s) 1833, 2007
Eco24I GRGCYC 2 cut(s) 986, 1757
Eco32I GATATC 1 cut(s) 1582
Eco47I GGWCC 7 cut(s) 110, 124, 500, 945, 1001, 1544, 1894
Eco53kI GAGCTC 1 cut(s) 984
Eco57I CTGAAG 4 cut(s) 574, 1230, 2243, 2265
Eco81I CCTNAGG 1 cut(s) 2229
EcoICRI GAGCTC 1 cut(s) 984
EcoO109I RGGNCCY 5 cut(s) 110, 945, 1001, 1544, 2240
EcoRI GAATTC 1 cut(s) 994
EcoRII CCWGG 5 cut(s) 1009, 1228, 1591, 1897, 2110
EcoRV GATATC 1 cut(s) 1582
EcoT14I CCWWGG 2 cut(s) 1833, 2007
EcoT38I GRGCYC 2 cut(s) 986, 1757
ErhI CCWWGG 2 cut(s) 1833, 2007
FalI AAGNNNNNCTT 2 cut(s) 1305, 1337
FaqI GGGAC 2 cut(s) 513, 1557
FauI CCCGC 1 cut(s) 1358
FauNDI CATATG 1 cut(s) 2119
FblI GTMKAC 1 cut(s) 1454
FokI GGATG 6 cut(s) 278, 508, 632, 1051, 1816, 2291
FriOI GRGCYC 2 cut(s) 986, 1757
FspBI CTAG 4 cut(s) 720, 1056, 2085, 2169
GlaI GCGC 4 cut(s) 69, 1330, 1590, 1948
GsaI CCCAGC 1 cut(s) 993
GsuI CTGGAG 2 cut(s) 1063, 2094
HaeII RGCGCY 2 cut(s) 1332, 1592
HaeIII GGCC 7 cut(s) 395, 466, 805, 848, 1633, 1838, 2242
HapII CCGG 8 cut(s) 391, 396, 467, 672, 1629, 1657, 1683, 2141
HgaI GACGC 1 cut(s) 1188
HhaI GCGC 4 cut(s) 70, 1331, 1591, 1949
Hin6I GCGC 4 cut(s) 68, 1329, 1589, 1947
HinP1I GCGC 4 cut(s) 68, 1329, 1589, 1947
HincII GTYRAC 3 cut(s) 625, 1310, 1455
HindII GTYRAC 3 cut(s) 625, 1310, 1455
HinfI GANTC 6 cut(s) 212, 470, 525, 1462, 1826, 2299
HpaI GTTAAC 1 cut(s) 625
HpaII CCGG 8 cut(s) 391, 396, 467, 672, 1629, 1657, 1683, 2141
HphI GGTGA 4 cut(s) 917, 991, 1023, 1733
Hpy166II GTNNAC 5 cut(s) 97, 625, 1310, 1455, 1796
Hpy188I TCNGA 8 cut(s) 285, 443, 578, 1210, 1423, 1644, 2223, 2325
Hpy188III TCNNGA 9 cut(s) 122, 143, 662, 1070, 1683, 1713, 1780, 2185, 2296
Hpy8I GTNNAC 5 cut(s) 97, 625, 1310, 1455, 1796
Hpy99I CGWCG 1 cut(s) 2105
HpyAV CCTTC 9 cut(s) 30, 406, 485, 958, 958, 1243, 1748, 2049, 2082
HpyCH4IV ACGT 2 cut(s) 2100, 2253
HpyF3I CTNAG 7 cut(s) 34, 100, 282, 889, 985, 1361, 2229
HpySE526I ACGT 2 cut(s) 2100, 2253
HspAI GCGC 4 cut(s) 68, 1329, 1589, 1947
KflI GGGWCCC 1 cut(s) 1544
Kpn2I TCCGGA 1 cut(s) 1682
KroI GCCGGC 1 cut(s) 395
KroNI GCCGGC 1 cut(s) 397
KspAI GTTAAC 1 cut(s) 625
Kzo9I GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
LmnI GCTCC 6 cut(s) 104, 578, 1052, 1118, 1519, 1752
LweI GCATC 4 cut(s) 823, 1029, 1117, 1171
MaeI CTAG 4 cut(s) 720, 1056, 2085, 2169
MaeII ACGT 2 cut(s) 2100, 2253
MaeIII GTNAC 8 cut(s) 151, 662, 1029, 1087, 2063, 2074, 2080, 2155
MalI GATC 6 cut(s) 141, 297, 575, 766, 1068, 1386
MboI GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
MflI RGATCY 2 cut(s) 295, 764
MhlI GDGCHC 6 cut(s) 208, 888, 986, 1703, 1757, 1817
MlyI GAGTC 2 cut(s) 1820, 2308
MmeI TCCRAC 2 cut(s) 1030, 1315
MroI TCCGGA 1 cut(s) 1682
MroNI GCCGGC 1 cut(s) 395
MroXI GAANNNNTTC 1 cut(s) 2207
MslI CAYNNNNRTG 3 cut(s) 514, 1999, 2072
MspA1I CMGCKG 2 cut(s) 535, 989
MspCI CTTAAG 1 cut(s) 1571
MspI CCGG 8 cut(s) 391, 396, 467, 672, 1629, 1657, 1683, 2141
MspR9I CCNGG 7 cut(s) 392, 1011, 1230, 1593, 1630, 1899, 2112
MvaI CCWGG 5 cut(s) 1011, 1230, 1593, 1899, 2112
MvnI CGCG 2 cut(s) 780, 1071
NaeI GCCGGC 1 cut(s) 397
NciI CCSGG 2 cut(s) 392, 1630
NdeI CATATG 1 cut(s) 2119
NdeII GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
NgoMIV GCCGGC 1 cut(s) 395
NheI GCTAGC 1 cut(s) 1055
NmuCI GTSAC 6 cut(s) 151, 662, 1029, 2063, 2074, 2080
NruI TCGCGA 1 cut(s) 1071
NspI RCATGY 4 cut(s) 74, 1179, 1412, 1874
OliI CACNNNNGTG 1 cut(s) 2072
PaeI GCATGC 2 cut(s) 74, 1179
PagI TCATGA 1 cut(s) 1779
PaqCI CACCTGC 1 cut(s) 692
PciI ACATGT 2 cut(s) 1408, 1870
PcsI WCGNNNNNNNCGW 2 cut(s) 819, 828
PdiI GCCGGC 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 2207
PfeI GAWTC 4 cut(s) 212, 470, 525, 1462
PflMI CCANNNNNTGG 1 cut(s) 815
PinAI ACCGGT 1 cut(s) 2140
PleI GAGTC 2 cut(s) 1820, 2307
PpsI GAGTC 2 cut(s) 1820, 2307
Ppu21I YACGTR 1 cut(s) 2254
PpuMI RGGWCCY 4 cut(s) 110, 945, 1001, 1544
PscI ACATGT 2 cut(s) 1408, 1870
PshBI ATTAAT 2 cut(s) 867, 1479
Psp124BI GAGCTC 1 cut(s) 986
Psp5II RGGWCCY 4 cut(s) 110, 945, 1001, 1544
Psp6I CCWGG 5 cut(s) 1009, 1228, 1591, 1897, 2110
PspFI CCCAGC 1 cut(s) 989
PspGI CCWGG 5 cut(s) 1009, 1228, 1591, 1897, 2110
PspPPI RGGWCCY 4 cut(s) 110, 945, 1001, 1544
PstI CTGCAG 2 cut(s) 637, 1762
PstNI CAGNNNCTG 2 cut(s) 1331, 2078
PsuI RGATCY 2 cut(s) 295, 764
PvuII CAGCTG 2 cut(s) 535, 989
RruI TCGCGA 1 cut(s) 1071
RsaI GTAC 3 cut(s) 98, 587, 2173
RsaNI GTAC 3 cut(s) 97, 586, 2172
RseI CAYNNNNRTG 3 cut(s) 514, 1999, 2072
SacI GAGCTC 1 cut(s) 986
SalI GTCGAC 1 cut(s) 1453
Sau3AI GATC 6 cut(s) 139, 295, 573, 764, 1066, 1384
SchI GAGTC 2 cut(s) 1820, 2308
ScrFI CCNGG 7 cut(s) 392, 1011, 1230, 1593, 1630, 1899, 2112
SduI GDGCHC 6 cut(s) 208, 888, 986, 1703, 1757, 1817
SfaNI GCATC 4 cut(s) 823, 1029, 1117, 1171
SfcI CTRYAG 4 cut(s) 338, 633, 1134, 1758
SinI GGWCC 7 cut(s) 110, 124, 500, 945, 1001, 1544, 1894
SmiMI CAYNNNNRTG 3 cut(s) 514, 1999, 2072
SmlI CTYRAG 3 cut(s) 1112, 1571, 1620
SmoI CTYRAG 3 cut(s) 1112, 1571, 1620
SnaBI TACGTA 1 cut(s) 2254
SphI GCATGC 2 cut(s) 74, 1179
SsiI CCGC 5 cut(s) 135, 849, 914, 1365, 1788
SspI AATATT 1 cut(s) 219
SspMI CTAG 4 cut(s) 720, 1056, 2085, 2169
SstI GAGCTC 1 cut(s) 986
StyD4I CCNGG 7 cut(s) 390, 1009, 1228, 1591, 1628, 1897, 2110
StyI CCWWGG 2 cut(s) 1833, 2007
TaiI ACGT 2 cut(s) 2103, 2256
TaqI TCGA 9 cut(s) 144, 367, 813, 831, 1022, 1239, 1279, 1454, 1650
TaqII GACCGA 1 cut(s) 1712
TatI WGTACW 1 cut(s) 96
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 4 cut(s) 212, 470, 525, 1462
TscAI CASTG 5 cut(s) 757, 897, 1023, 2079, 2197
TseFI GTSAC 6 cut(s) 151, 662, 1029, 2063, 2074, 2080
Tsp45I GTSAC 6 cut(s) 151, 662, 1029, 2063, 2074, 2080
TspDTI ATGAA 7 cut(s) 47, 213, 285, 342, 504, 1386, 1518
TspGWI ACGGA 3 cut(s) 1440, 2051, 2291
TspRI CASTG 5 cut(s) 757, 897, 1023, 2079, 2197
Van91I CCANNNNNTGG 1 cut(s) 815
Vha464I CTTAAG 1 cut(s) 1571
VpaK11BI GGWCC 7 cut(s) 110, 124, 500, 945, 1001, 1544, 1894
VspI ATTAAT 2 cut(s) 867, 1479
XapI RAATTY 4 cut(s) 7, 157, 994, 1263
XceI RCATGY 4 cut(s) 74, 1179, 1412, 1874
XcmI CCANNNNNNNNNTGG 2 cut(s) 674, 1465
XmiI GTMKAC 1 cut(s) 1454
XmnI GAANNNNTTC 1 cut(s) 2207
XspI CTAG 4 cut(s) 720, 1056, 2085, 2169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.