Rh5BG516200

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000923
Physical Location & Seq
Reverse (-)
81738721 .. 81743010
4290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG516200.1

Sequence Viewer

Length: 2343 bp
ATGGGGCATTGTTCACGTCTCCCTCTTTTTCTTCTATCTCTTCTTCTCCTCTCTCTGTTTCAGACGCCAGCCGATGCAGCCAAACAGTCTTACATTGTGTACTTGGGAACACATTCCCATGGCTTGAACCCTACGTCTGTCGAACTCGAAGCTGCAACGAATTCTCAGTACAATCTGCTTGGAACTGTTTTGGGAAGCAATGAGAGGGCCCAAGAAGCATTGTTTTACTCATATACTAGAGAGATCAATGGATTTGCTGCAGTTTTAGAAGAAGAAGAGGCTTCACAGATTGCAAAGGATCCAAGTGTTGTGTCTGTTTTTCCAAACAGAAAATTAAAACTACATACAACTCATTCATGGGAGTTCCTGGGACTAGAAGACCAAAATGGAGTACCTCGTCACGGATCGATTTGGAAGAAAGCTAAGTTTGGTGAAAATACAATCATTGGAAACCTTGACACTGGTGTCTGGCCGGAATCACCGAGCTTTAGCGATGAAGGGTTTGGACCAATCCCATCTAAGTGGCGTGGCGCTTGTCAGCTTGACACCAAAGACAAAGTTCGTTGCAACAGGAAGCTGATCGGAGCAAGGTACTTTAACAAAGGCTTCTTGGAGTCTGCCAGGCTCAATAATGCCACCATTCCACCAGAAATTCTGGTGTCACCGCGTGACCAGGAAGGCCATGGCTCACACACCCTTTCAACAGCTGGTGGCATTTATGTGCCGGGGGCTAATGTTTTTGGTCATGGCAATGGCACTGCTAAAGGTGGATCCCCAAAAGCTCGCGTGGCTGCTTACAAAGTATGTTGGCCAAGCTTTGGCAATACAGAAGGAGGGTGTACTAATGCAGACATCATGGCCGCCTTTGATGCTGCAATCAGTGACGGTGTTGATGTTCTTTCGCTGTCCGTTGGTGGTGATTCTGTAGAGTTTTTTGAGGATGGGATCGCAATAGGGTCATTCCATGCGGCTAAGCATGGAATCGTTGTTGTTTGCTCAGCTGGCAATTCAGGGCCTGCCCAAGGAACAGTGACTAATGTGGCACCCTGGATATTAACAGTTGGAGCTAGCACGATTGATCGCGAGTTCACTAGTTATTTATCCTTTGGAAAGGGGAAGCATTTCAAGGGAAAAAGTCTTTCTTCCAGAAGTTTTGCATCTAAGAAGTTTTATCCTGTGATAAGTGGAGCCAATGCTAATGCAGCTACTTCGCCTCCTGAAGATTCCTTGCTATGTTTGAATGGAACCCTTGATCCAAAGAAGGTCAAGGGGAAGATCTTGGTCTGCCTCCGCGGGCAGAATGGGAGAACTGAAAAAGCCAATATAGCTGCTCTTGCCGGCGCTGTTGGAATGGTTTTGGTTAACGATGAGCAAAGTGGAGATGATCTTATGGCTGATCCTTATCTGCTCCCCGCTACACATCTGACTTACACTGATGGAAAACGTGTCTTTGAGTACCTGAATTCTACCAAGAACCCTTTTGGTCACATTACTCCTGTTAAGACGGTAGAAGGAACAAAGCCAGCTCCATTTATGGCTTCATTCTCATCTCGTGGACCGAGTATCATTGAGCCATCAATCCTTAAGCCTGATATCACAGCACCAGGAGTGGATATAATTGCTGCTTATACTGGAGCACCTCTCCCAAGTGATTCGGACATTGGTAACCGTCGCCCACTTTACATTACAGAATCAGGAACATCTATGTCATGCCCTCATGTATCTGGAATAGTCGGTCTTCTGAAAACACTTTACCCGTCATGGAGCCCCGCTGCTATTAAGTCTGCAATCTTGACCACAGCGAGAAGACGCGACAACAAACGGGAGTCAATTCTTGATTCAACCAAGGTCTCAGCTACACCACTTGACTATGGTGCAGGCCATGTTGATCCAAATCGCGCAGCAAACCCCGGACTTGTCTATGACTTGACTACCGATGATTACTTGAATTTCTTATGTGCTCGTGGATACAATGCATCACTTCTCAAAATATTTTCCGACAAGCCACACAAATGTTCCAACACCTCTAGCATTGTTGCTGATTTAAACTATCCTACAATTGCTGTTCCTGATCTCCAAGACAAACCAATTACCATTTCTAGAAAAGTGAAGAATGTCGGTACTCCAGGCACATATGTAGCATATGTTCGTGCACCAGCTAGAGTTTCTGTTTCGGTTCAGCCTAGCAGGTTGAAATTCAAGTCCATTGGTGAAGAGAAGGAATTCAAGGTTACGCTGAAGGCAGATGGTAAGGTTCGAAATCCAGACTATGTGTTTGGAGAGTTGAAATGGTCGGATGGCAAGCACACTGTGAGGAGTCCTATTGCTGTGATGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

780

Amino Acids

83.64

Weight (kDa)

8.82

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 30 - 115 5.9e-17 Peptidase inhibitor I9
MORF_dom PF21864 58 - 114 1.8e-06 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 144 - 606 4.8e-40 Subtilase family
PA PF02225 407 - 482 3.1e-13 PA domain
fn3_6 PF17766 681 - 777 2.8e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 2178
AccB1I GGYRCC 1 cut(s) 1042
AccB7I CCANNNNNTGG 1 cut(s) 818
AccII CGCG 6 cut(s) 667, 786, 1083, 1293, 1812, 1899
AciI CCGC 7 cut(s) 665, 861, 968, 1291, 1293, 1413, 1770
AclWI GGATC 9 cut(s) 293, 306, 412, 765, 778, 953, 1247, 1391, 1883
AcoI YGGCCR 3 cut(s) 470, 809, 858
AcsI RAATTY 6 cut(s) 160, 651, 1462, 1948, 2195, 2222
AcuI CTGAAG 2 cut(s) 1239, 2258
AcyI GRCGYC 1 cut(s) 65
AdeI CACNNNGTG 2 cut(s) 668, 2311
AfaI GTAC 7 cut(s) 101, 170, 393, 593, 841, 1457, 2122
AfiI CCNNNNNNNGG 3 cut(s) 401, 818, 1022
AflII CTTAAG 1 cut(s) 1583
AflIII ACRYGT 1 cut(s) 1444
AhlI ACTAGT 1 cut(s) 1091
AjiI CACGTC 1 cut(s) 17
AjnI CCWGG 6 cut(s) 366, 620, 672, 1046, 1603, 2125
AjuI GAANNNNNNNTTGG 2 cut(s) 2080, 2112
AloI GAACNNNNNNTCC 2 cut(s) 1237, 1269
Alw21I GWGCWC 3 cut(s) 1639, 1963, 2155
Alw26I GTCTC 2 cut(s) 23, 1855
Alw44I GTGCAC 1 cut(s) 2151
AlwI GGATC 9 cut(s) 293, 306, 412, 765, 778, 953, 1247, 1391, 1883
AlwNI CAGNNNCTG 1 cut(s) 1016
AoxI GGCC 7 cut(s) 207, 470, 679, 809, 858, 1013, 1879
ApaI GGGCCC 1 cut(s) 211
ApaLI GTGCAC 1 cut(s) 2151
ApoI RAATTY 6 cut(s) 160, 651, 1462, 1948, 2195, 2222
ArsI GACNNNNNNTTYG 2 cut(s) 2258, 2290
Asp700I GAANNNNTTC 3 cut(s) 112, 1121, 2222
AspLEI GCGC 3 cut(s) 533, 1343, 1901
AspS9I GGNCC 5 cut(s) 207, 208, 506, 1013, 1556
AsuC2I CCSGG 2 cut(s) 726, 1911
AsuHPI GGTGA 5 cut(s) 443, 471, 654, 929, 2222
AsuII TTCGAA 1 cut(s) 2257
AsuNHI GCTAGC 1 cut(s) 1067
AvaII GGWCC 2 cut(s) 506, 1556
BaeGI GKGCMC 2 cut(s) 211, 2155
BalI TGGCCA 1 cut(s) 811
BamHI GGATCC 2 cut(s) 298, 770
BanI GGYRCC 1 cut(s) 1042
BanII GRGCYC 2 cut(s) 211, 1769
BauI CACGAG 2 cut(s) 1551, 1962
BbsI GAAGAC 3 cut(s) 384, 1730, 1813
Bbv12I GWGCWC 3 cut(s) 1639, 1963, 2155
BccI CCATC 6 cut(s) 523, 935, 1430, 1582, 2240, 2291
BcgI CGANNNNNNTGC 2 cut(s) 1191, 1225
BciT130I CCWGG 6 cut(s) 368, 622, 674, 1048, 1605, 2127
BciVI GTATCC 1 cut(s) 1961
BcnI CCSGG 2 cut(s) 726, 1911
BcoDI GTCTC 2 cut(s) 23, 1855
BcuI ACTAGT 1 cut(s) 1091
BfaI CTAG 9 cut(s) 237, 374, 1068, 1092, 2028, 2100, 2160, 2184, 2341
BfmI CTRYAG 2 cut(s) 258, 924
BfoI RGCGCY 2 cut(s) 534, 1344
BfrI CTTAAG 1 cut(s) 1583
BfuAI ACCTGC 1 cut(s) 2178
BfuI GTATCC 1 cut(s) 1961
BglII AGATCT 1 cut(s) 1275
BlpI GCTNAGC 2 cut(s) 972, 997
Bme1390I CCNGG 8 cut(s) 368, 622, 674, 726, 1048, 1605, 1911, 2127
Bme18I GGWCC 2 cut(s) 506, 1556
BmgBI CACGTC 1 cut(s) 17
BmgT120I GGNCC 5 cut(s) 207, 208, 506, 1013, 1556
BmiI GGNNCC 7 cut(s) 209, 300, 772, 1044, 1189, 1246, 1766
BmrFI CCNGG 8 cut(s) 368, 622, 674, 726, 1048, 1605, 1911, 2127
BmsI GCATC 5 cut(s) 64, 859, 1166, 1985, 2322
BmtI GCTAGC 1 cut(s) 1071
BpiI GAAGAC 3 cut(s) 384, 1730, 1813
BplI GAGNNNNNCTC 2 cut(s) 1626, 1658
BpmI CTGGAG 2 cut(s) 1653, 2109
Bpu1102I GCTNAGC 2 cut(s) 972, 997
Bpu14I TTCGAA 1 cut(s) 2257
BpuMI CCSGG 2 cut(s) 726, 1911
Bsa29I ATCGAT 1 cut(s) 407
BsaBI GATNNNNATC 2 cut(s) 1401, 1893
BsaHI GRCGYC 1 cut(s) 65
BsaI GGTCTC 1 cut(s) 1855
BsaJI CCNNGG 9 cut(s) 118, 367, 682, 725, 1021, 1046, 1291, 1845, 1909
BsaXI ACNNNNNCTCC 2 cut(s) 1198, 1228
Bsc4I CCNNNNNNNGG 3 cut(s) 401, 818, 1022
Bse118I RCCGGY 1 cut(s) 1337
Bse1I ACTGG 2 cut(s) 466, 1636
Bse3DI GCAATG 2 cut(s) 205, 757
Bse8I GATNNNNATC 2 cut(s) 1401, 1893
BseBI CCWGG 6 cut(s) 368, 622, 674, 1048, 1605, 2127
BseCI ATCGAT 1 cut(s) 407
BseDI CCNNGG 9 cut(s) 118, 367, 682, 725, 1021, 1046, 1291, 1845, 1909
BseGI GGATG 2 cut(s) 946, 2302
BseJI GATNNNNATC 2 cut(s) 1401, 1893
BseLI CCNNNNNNNGG 3 cut(s) 401, 818, 1022
BseMI GCAATG 2 cut(s) 205, 757
BseMII CTCAG 3 cut(s) 179, 1011, 1866
BseNI ACTGG 2 cut(s) 466, 1636
BseRI GAGGAG 2 cut(s) 38, 2329
BseSI GKGCMC 2 cut(s) 211, 2155
BsgI GTGCAG 1 cut(s) 1896
Bsh1236I CGCG 6 cut(s) 667, 786, 1083, 1293, 1812, 1899
BshFI GGCC 7 cut(s) 209, 472, 681, 811, 860, 1015, 1881
BshNI GGYRCC 1 cut(s) 1042
BshVI ATCGAT 1 cut(s) 407
BsiHKAI GWGCWC 3 cut(s) 1639, 1963, 2155
BsiSI CCGG 4 cut(s) 473, 725, 1338, 1911
BslFI GGGAC 1 cut(s) 384
BslI CCNNNNNNNGG 3 cut(s) 401, 818, 1022
BsmAI GTCTC 2 cut(s) 23, 1855
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 384
BsnI GGCC 7 cut(s) 209, 472, 681, 811, 860, 1015, 1881
Bso31I GGTCTC 1 cut(s) 1855
Bsp119I TTCGAA 1 cut(s) 2257
Bsp120I GGGCCC 1 cut(s) 207
Bsp1286I GDGCHC 5 cut(s) 211, 1639, 1769, 1963, 2155
Bsp1720I GCTNAGC 2 cut(s) 972, 997
Bsp19I CCATGG 2 cut(s) 118, 682
Bsp68I TCGCGA 1 cut(s) 1083
BspACI CCGC 7 cut(s) 665, 861, 968, 1291, 1293, 1413, 1770
BspANI GGCC 7 cut(s) 209, 472, 681, 811, 860, 1015, 1881
BspCNI CTCAG 3 cut(s) 178, 1010, 1865
BspDI ATCGAT 1 cut(s) 407
BspFNI CGCG 6 cut(s) 667, 786, 1083, 1293, 1812, 1899
BspLI GGNNCC 7 cut(s) 209, 300, 772, 1044, 1189, 1246, 1766
BspMAI CTGCAG 1 cut(s) 262
BspMI ACCTGC 1 cut(s) 2178
BspOI GCTAGC 1 cut(s) 1071
BspPI GGATC 9 cut(s) 293, 306, 412, 765, 778, 953, 1247, 1391, 1883
BspT104I TTCGAA 1 cut(s) 2257
BspT107I GGYRCC 1 cut(s) 1042
BspTI CTTAAG 1 cut(s) 1583
BspTNI GGTCTC 1 cut(s) 1855
BsrDI GCAATG 2 cut(s) 205, 757
BsrFI RCCGGY 1 cut(s) 1337
BsrI ACTGG 2 cut(s) 466, 1636
BssAI RCCGGY 1 cut(s) 1337
BssECI CCNNGG 9 cut(s) 118, 367, 682, 725, 1021, 1046, 1291, 1845, 1909
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 2 cut(s) 1551, 1962
BssT1I CCWWGG 4 cut(s) 118, 682, 1021, 1845
Bst2BI CACGAG 2 cut(s) 1551, 1962
Bst2UI CCWGG 6 cut(s) 368, 622, 674, 1048, 1605, 2127
Bst4CI ACNGT 8 cut(s) 87, 187, 887, 1030, 1060, 1507, 1670, 2311
Bst6I CTCTTC 3 cut(s) 45, 270, 2208
BstACI GRCGYC 1 cut(s) 65
BstAFI CTTAAG 1 cut(s) 1583
BstBI TTCGAA 1 cut(s) 2257
BstDEI CTNAG 7 cut(s) 165, 423, 519, 972, 997, 1161, 1852
BstDSI CCRYGG 3 cut(s) 118, 682, 1291
BstEII GGTNACC 1 cut(s) 1664
BstENI CCTNNNNNAGG 1 cut(s) 1020
BstF5I GGATG 2 cut(s) 946, 2302
BstFNI CGCG 6 cut(s) 667, 786, 1083, 1293, 1812, 1899
BstH2I RGCGCY 2 cut(s) 534, 1344
BstHHI GCGC 3 cut(s) 533, 1343, 1901
BstMAI GTCTC 2 cut(s) 23, 1855
BstMWI GCNNNNNNNGC 8 cut(s) 77, 215, 788, 869, 1002, 1202, 1325, 1334
BstNI CCWGG 6 cut(s) 368, 622, 674, 1048, 1605, 2127
BstPI GGTNACC 1 cut(s) 1664
BstSCI CCNGG 8 cut(s) 366, 620, 672, 724, 1046, 1603, 1909, 2125
BstSFI CTRYAG 2 cut(s) 258, 924
BstSLI GKGCMC 2 cut(s) 211, 2155
BstUI CGCG 6 cut(s) 667, 786, 1083, 1293, 1812, 1899
BstV2I GAAGAC 3 cut(s) 384, 1730, 1813
BstX2I RGATCY 3 cut(s) 298, 770, 1275
BstXI CCANNNNNNTGG 1 cut(s) 522
BstYI RGATCY 3 cut(s) 298, 770, 1275
Bsu15I ATCGAT 1 cut(s) 407
BsuI GTATCC 1 cut(s) 1961
BsuRI GGCC 7 cut(s) 209, 472, 681, 811, 860, 1015, 1881
BsuTUI ATCGAT 1 cut(s) 407
BtgI CCRYGG 3 cut(s) 118, 682, 1291
BtgZI GCGATG 1 cut(s) 507
BtrI CACGTC 1 cut(s) 17
BtsCI GGATG 2 cut(s) 946, 2302
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 6 cut(s) 459, 756, 886, 1035, 1431, 2307
BtuMI TCGCGA 1 cut(s) 1083
BveI ACCTGC 1 cut(s) 2178
CaiI CAGNNNCTG 1 cut(s) 1016
CfoI GCGC 3 cut(s) 533, 1343, 1901
Cfr10I RCCGGY 1 cut(s) 1337
Cfr13I GGNCC 5 cut(s) 207, 208, 506, 1013, 1556
Cfr42I CCGCGG 1 cut(s) 1294
ClaI ATCGAT 1 cut(s) 407
CseI GACGC 3 cut(s) 73, 1818, 2324
Csp6I GTAC 7 cut(s) 100, 169, 392, 592, 840, 1456, 2121
CviQI GTAC 7 cut(s) 100, 169, 392, 592, 840, 1456, 2121
DdeI CTNAG 7 cut(s) 165, 423, 519, 972, 997, 1161, 1852
DraI TTTAAA 1 cut(s) 2046
DraIII CACNNNGTG 2 cut(s) 668, 2311
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
EaeI YGGCCR 3 cut(s) 470, 809, 858
Eam1104I CTCTTC 3 cut(s) 45, 270, 2208
EarI CTCTTC 3 cut(s) 45, 270, 2208
Eco130I CCWWGG 4 cut(s) 118, 682, 1021, 1845
Eco24I GRGCYC 2 cut(s) 211, 1769
Eco31I GGTCTC 1 cut(s) 1855
Eco32I GATATC 1 cut(s) 1594
Eco47I GGWCC 2 cut(s) 506, 1556
Eco57I CTGAAG 2 cut(s) 1239, 2258
Eco91I GGTNACC 1 cut(s) 1664
EcoNI CCTNNNNNAGG 1 cut(s) 1020
EcoO109I RGGNCCY 2 cut(s) 207, 1013
EcoO65I GGTNACC 1 cut(s) 1664
EcoRI GAATTC 3 cut(s) 160, 1462, 2222
EcoRII CCWGG 6 cut(s) 366, 620, 672, 1046, 1603, 2125
EcoRV GATATC 1 cut(s) 1594
EcoT14I CCWWGG 4 cut(s) 118, 682, 1021, 1845
EcoT22I ATGCAT 1 cut(s) 1978
EcoT38I GRGCYC 2 cut(s) 211, 1769
ErhI CCWWGG 4 cut(s) 118, 682, 1021, 1845
Esp3I CGTCTC 1 cut(s) 23
FalI AAGNNNNNCTT 2 cut(s) 1126, 1158
FaqI GGGAC 1 cut(s) 384
FauI CCCGC 3 cut(s) 1286, 1420, 1777
FauNDI CATATG 2 cut(s) 2134, 2143
FokI GGATG 2 cut(s) 953, 2309
FriOI GRGCYC 2 cut(s) 211, 1769
FspBI CTAG 9 cut(s) 237, 374, 1068, 1092, 2028, 2100, 2160, 2184, 2341
GlaI GCGC 3 cut(s) 532, 1342, 1900
GsuI CTGGAG 2 cut(s) 1653, 2109
HaeII RGCGCY 2 cut(s) 534, 1344
HaeIII GGCC 7 cut(s) 209, 472, 681, 811, 860, 1015, 1881
HapII CCGG 4 cut(s) 473, 725, 1338, 1911
HgaI GACGC 3 cut(s) 73, 1818, 2324
HhaI GCGC 3 cut(s) 533, 1343, 1901
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 3 cut(s) 531, 1341, 1899
HinP1I GCGC 3 cut(s) 531, 1341, 1899
HincII GTYRAC 1 cut(s) 1363
HindII GTYRAC 1 cut(s) 1363
HindIII AAGCTT 1 cut(s) 814
HpaI GTTAAC 1 cut(s) 1363
HpaII CCGG 4 cut(s) 473, 725, 1338, 1911
HphI GGTGA 5 cut(s) 443, 471, 654, 929, 2222
Hpy166II GTNNAC 7 cut(s) 14, 100, 840, 1089, 1363, 1556, 2153
Hpy188I TCNGA 7 cut(s) 63, 584, 1425, 1657, 1743, 1999, 2296
Hpy8I GTNNAC 7 cut(s) 14, 100, 840, 1089, 1363, 1556, 2153
Hpy99I CGWCG 1 cut(s) 1674
HpyAV CCTTC 7 cut(s) 491, 671, 824, 1255, 1505, 2212, 2233
HpyCH4III ACNGT 8 cut(s) 87, 187, 887, 1030, 1060, 1507, 1670, 2311
HpyCH4IV ACGT 3 cut(s) 16, 134, 1444
HpyF10VI GCNNNNNNNGC 8 cut(s) 77, 215, 788, 869, 1002, 1202, 1325, 1334
HpyF3I CTNAG 7 cut(s) 165, 423, 519, 972, 997, 1161, 1852
HpySE526I ACGT 3 cut(s) 16, 134, 1444
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 3 cut(s) 531, 1341, 1899
KroI GCCGGC 1 cut(s) 1337
KroNI GCCGGC 1 cut(s) 1339
KspAI GTTAAC 1 cut(s) 1363
KspI CCGCGG 1 cut(s) 1294
LmnI GCTCC 7 cut(s) 584, 1064, 1187, 1413, 1531, 1634, 1764
LweI GCATC 5 cut(s) 64, 859, 1166, 1985, 2322
MaeI CTAG 9 cut(s) 237, 374, 1068, 1092, 2028, 2100, 2160, 2184, 2341
MaeII ACGT 3 cut(s) 16, 134, 1444
MaeIII GTNAC 9 cut(s) 398, 660, 668, 881, 1030, 1484, 1664, 2230, 2336
MfeI CAATTG 1 cut(s) 2058
MflI RGATCY 3 cut(s) 298, 770, 1275
MhlI GDGCHC 5 cut(s) 211, 1639, 1769, 1963, 2155
MlsI TGGCCA 1 cut(s) 811
MluNI TGGCCA 1 cut(s) 811
MlyI GAGTC 3 cut(s) 623, 1835, 2326
MmeI TCCRAC 5 cut(s) 1042, 1327, 2022, 2043, 2274
Mox20I TGGCCA 1 cut(s) 811
Mph1103I ATGCAT 1 cut(s) 1978
MroNI GCCGGC 1 cut(s) 1337
MroXI GAANNNNTTC 3 cut(s) 112, 1121, 2222
MscI TGGCCA 1 cut(s) 811
MseI TTAA 8 cut(s) 335, 597, 1055, 1362, 1500, 1584, 1779, 2045
MslI CAYNNNNRTG 5 cut(s) 117, 520, 719, 750, 2011
Msp20I TGGCCA 1 cut(s) 811
MspA1I CMGCKG 4 cut(s) 707, 1001, 1293, 1772
MspCI CTTAAG 1 cut(s) 1583
MspI CCGG 4 cut(s) 473, 725, 1338, 1911
MspR9I CCNGG 8 cut(s) 368, 622, 674, 726, 1048, 1605, 1911, 2127
MunI CAATTG 1 cut(s) 2058
MvaI CCWGG 6 cut(s) 368, 622, 674, 1048, 1605, 2127
MvnI CGCG 6 cut(s) 667, 786, 1083, 1293, 1812, 1899
MwoI GCNNNNNNNGC 8 cut(s) 77, 215, 788, 869, 1002, 1202, 1325, 1334
NaeI GCCGGC 1 cut(s) 1339
NciI CCSGG 2 cut(s) 726, 1911
NcoI CCATGG 2 cut(s) 118, 682
NdeI CATATG 2 cut(s) 2134, 2143
NgoMIV GCCGGC 1 cut(s) 1337
NheI GCTAGC 1 cut(s) 1067
NlaIV GGNNCC 7 cut(s) 209, 300, 772, 1044, 1189, 1246, 1766
NmuCI GTSAC 7 cut(s) 398, 660, 668, 881, 1030, 1484, 2336
NruI TCGCGA 1 cut(s) 1083
NsiI ATGCAT 1 cut(s) 1978
NspV TTCGAA 1 cut(s) 2257
PdiI GCCGGC 1 cut(s) 1339
PdmI GAANNNNTTC 3 cut(s) 112, 1121, 2222
PfeI GAWTC 7 cut(s) 476, 920, 981, 1223, 1652, 1691, 1838
PflMI CCANNNNNTGG 1 cut(s) 818
PleI GAGTC 3 cut(s) 622, 1834, 2325
PpsI GAGTC 3 cut(s) 622, 1834, 2325
Psp6I CCWGG 6 cut(s) 366, 620, 672, 1046, 1603, 2125
PspEI GGTNACC 1 cut(s) 1664
PspGI CCWGG 6 cut(s) 366, 620, 672, 1046, 1603, 2125
PspN4I GGNNCC 7 cut(s) 209, 300, 772, 1044, 1189, 1246, 1766
PspOMI GGGCCC 1 cut(s) 207
PspPI GGNCC 5 cut(s) 207, 208, 506, 1013, 1556
PsrI GAACNNNNNNTAC 2 cut(s) 2130, 2162
PstI CTGCAG 1 cut(s) 262
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 3 cut(s) 298, 770, 1275
PvuII CAGCTG 2 cut(s) 707, 1001
RruI TCGCGA 1 cut(s) 1083
RsaI GTAC 7 cut(s) 101, 170, 393, 593, 841, 1457, 2122
RsaNI GTAC 7 cut(s) 100, 169, 392, 592, 840, 1456, 2121
RseI CAYNNNNRTG 5 cut(s) 117, 520, 719, 750, 2011
SacII CCGCGG 1 cut(s) 1294
SaqAI TTAA 8 cut(s) 335, 597, 1055, 1362, 1500, 1584, 1779, 2045
Sau96I GGNCC 5 cut(s) 207, 208, 506, 1013, 1556
SchI GAGTC 3 cut(s) 623, 1835, 2326
ScrFI CCNGG 8 cut(s) 368, 622, 674, 726, 1048, 1605, 1911, 2127
SduI GDGCHC 5 cut(s) 211, 1639, 1769, 1963, 2155
SfaNI GCATC 5 cut(s) 64, 859, 1166, 1985, 2322
SfcI CTRYAG 2 cut(s) 258, 924
Sfr303I CCGCGG 1 cut(s) 1294
SfuI TTCGAA 1 cut(s) 2257
SgrBI CCGCGG 1 cut(s) 1294
SinI GGWCC 2 cut(s) 506, 1556
SmiMI CAYNNNNRTG 5 cut(s) 117, 520, 719, 750, 2011
SmlI CTYRAG 1 cut(s) 1583
SmoI CTYRAG 1 cut(s) 1583
SpeI ACTAGT 1 cut(s) 1091
SsiI CCGC 7 cut(s) 665, 861, 968, 1291, 1293, 1413, 1770
SspI AATATT 1 cut(s) 1992
SspMI CTAG 9 cut(s) 237, 374, 1068, 1092, 2028, 2100, 2160, 2184, 2341
StyD4I CCNGG 8 cut(s) 366, 620, 672, 724, 1046, 1603, 1909, 2125
StyI CCWWGG 4 cut(s) 118, 682, 1021, 1845
TaaI ACNGT 8 cut(s) 87, 187, 887, 1030, 1060, 1507, 1670, 2311
TaiI ACGT 3 cut(s) 19, 137, 1447
TaqI TCGA 4 cut(s) 141, 147, 407, 2257
TaqII GACCGA 2 cut(s) 1573, 1724
TatI WGTACW 3 cut(s) 99, 168, 839
TauI GCSGC 2 cut(s) 863, 971
TfiI GAWTC 7 cut(s) 476, 920, 981, 1223, 1652, 1691, 1838
Tru1I TTAA 8 cut(s) 335, 597, 1055, 1362, 1500, 1584, 1779, 2045
Tru9I TTAA 8 cut(s) 335, 597, 1055, 1362, 1500, 1584, 1779, 2045
TscAI CASTG 6 cut(s) 466, 763, 886, 1035, 1438, 2314
TseFI GTSAC 7 cut(s) 398, 660, 668, 881, 1030, 1484, 2336
Tsp45I GTSAC 7 cut(s) 398, 660, 668, 881, 1030, 1484, 2336
TspDTI ATGAA 3 cut(s) 345, 510, 1530
TspGWI ACGGA 2 cut(s) 417, 898
TspRI CASTG 6 cut(s) 466, 763, 886, 1035, 1438, 2314
Van91I CCANNNNNTGG 1 cut(s) 818
Vha464I CTTAAG 1 cut(s) 1583
VneI GTGCAC 1 cut(s) 2151
VpaK11BI GGWCC 2 cut(s) 506, 1556
XagI CCTNNNNNAGG 1 cut(s) 1020
XapI RAATTY 6 cut(s) 160, 651, 1462, 1948, 2195, 2222
XbaI TCTAGA 1 cut(s) 2099
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XmnI GAANNNNTTC 3 cut(s) 112, 1121, 2222
XspI CTAG 9 cut(s) 237, 374, 1068, 1092, 2028, 2100, 2160, 2184, 2341
Zsp2I ATGCAT 1 cut(s) 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.