Rroxscaffold_3G00267190

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000923
Physical Location & Seq
Reverse (-)
60278435 .. 60280934
2500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267190.1

Sequence Viewer

Length: 636 bp
ATGAAGGAACTCATCAAGTTGCAATTTGGAGAAGAAATCTTAGATGAGTGGTTTGACTTGTACTCCAAGAAAATTGAACAGCAACGGTCAATCCTCGAATCAGGGAAGATACAGGCAGACAGCCCGGATTTCTTGTCCCCAAGAGATGTTGTTGGACATGGAACACACACATCTAGAATTGCTGGTGGCTCCTTTGTGTACAATGCAAGCTACAAAGGGATTGGCCTTGGAATAATCAGAGGTGGAGCTCCCCATGCTCGTTTGGCTATCTACAAAGTGTGCTGGAATGTGCCAAGAGGACAGTGCTCAAATGCTGACATGCTGAAAGCTTTTGATGATGCAATTCATGATGGAGTTGATGTTATATCAGTATCTCTTGGCACTCAACTTCCACTGTTTTCAGAGGTTGATGACCGCGATGCAATTTCCATTGGTTCATTCCATGCTGTTACTAAAGGCATACCTGTTGTTGGTGGAGCTGCAAATGAAGGACCTTCTGCATACACTGTCGAAAACACAGCACCTTGGATCTTAACTGTAGCAGCTTCTACTATTGATCGGTCTTTTCCTACAAATATTACACTAGGGAACAAATCAACCATACTGGATGTTTCTACTGAAGAATACGGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

211

Amino Acids

22.73

Weight (kDa)

5.06

Isoelectric Point (pI)

31.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 46 - 186 6.6e-14 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 417
AciI CCGC 1 cut(s) 415
AclWI GGATC 1 cut(s) 536
AfaI GTAC 2 cut(s) 62, 200
AfiI CCNNNNNNNGG 1 cut(s) 470
AgsI TTSAA 1 cut(s) 77
AluBI AGCT 5 cut(s) 210, 248, 329, 479, 545
AluI AGCT 5 cut(s) 210, 248, 329, 479, 545
Alw21I GWGCWC 2 cut(s) 250, 308
AlwI GGATC 1 cut(s) 536
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 2 cut(s) 479, 542
AspS9I GGNCC 1 cut(s) 491
AsuC2I CCSGG 1 cut(s) 125
AvaII GGWCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 250, 308
BbvI GCAGC 2 cut(s) 466, 554
BccI CCATC 1 cut(s) 344
BcnI CCSGG 1 cut(s) 125
BfaI CTAG 2 cut(s) 174, 584
BfmI CTRYAG 1 cut(s) 537
BisI GCNGC 2 cut(s) 480, 543
BlsI GCNGC 2 cut(s) 481, 544
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 1 cut(s) 190
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 2 cut(s) 328, 409
BpuMI CCSGG 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 226, 524
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 345, 375
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 609
BseDI CCNNGG 2 cut(s) 226, 524
BseGI GGATG 1 cut(s) 613
BseLI CCNNNNNNNGG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 609
BseXI GCAGC 2 cut(s) 466, 554
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 250, 308
BsiSI CCGG 1 cut(s) 125
BslFI GGGAC 1 cut(s) 121
BslI CCNNNNNNNGG 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 121
BsnI GGCC 1 cut(s) 225
Bsp1286I GDGCHC 2 cut(s) 250, 308
Bsp1407I TGTACA 1 cut(s) 198
Bsp143I GATC 2 cut(s) 528, 556
BspACI CCGC 1 cut(s) 415
BspANI GGCC 1 cut(s) 225
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 1 cut(s) 190
BspPI GGATC 1 cut(s) 536
BsrGI TGTACA 1 cut(s) 198
BsrI ACTGG 1 cut(s) 609
BssECI CCNNGG 2 cut(s) 226, 524
BssMI GATC 2 cut(s) 528, 556
BssT1I CCWWGG 2 cut(s) 226, 524
Bst4CI ACNGT 6 cut(s) 87, 303, 396, 508, 538, 629
BstAUI TGTACA 1 cut(s) 198
BstC8I GCNNGC 1 cut(s) 208
BstDEI CTNAG 1 cut(s) 40
BstF5I GGATG 1 cut(s) 613
BstFNI CGCG 1 cut(s) 417
BstKTI GATC 2 cut(s) 531, 559
BstMBI GATC 2 cut(s) 528, 556
BstMWI GCNNNNNNNGC 2 cut(s) 254, 263
BstNSI RCATGY 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 537
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 2 cut(s) 466, 554
BstX2I RGATCY 1 cut(s) 528
BstYI RGATCY 1 cut(s) 528
BsuRI GGCC 1 cut(s) 225
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 1 cut(s) 613
BtsIMutI CAGTG 3 cut(s) 308, 392, 504
Cac8I GCNNGC 1 cut(s) 208
CciI TCATGA 1 cut(s) 346
Cfr13I GGNCC 1 cut(s) 491
Csp6I GTAC 2 cut(s) 61, 199
CviAII CATG 5 cut(s) 158, 254, 319, 347, 443
CviJI RGCY 9 cut(s) 123, 189, 210, 225, 248, 266, 329, 479, 545
CviKI_1 RGCY 9 cut(s) 123, 189, 210, 225, 248, 266, 329, 479, 545
CviQI GTAC 2 cut(s) 61, 199
DdeI CTNAG 1 cut(s) 40
DpnI GATC 2 cut(s) 530, 558
DpnII GATC 2 cut(s) 528, 556
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 2 cut(s) 226, 524
Eco24I GRGCYC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 248
EcoO109I RGGNCCY 1 cut(s) 491
EcoT14I CCWWGG 2 cut(s) 226, 524
EcoT38I GRGCYC 1 cut(s) 250
ErhI CCWWGG 2 cut(s) 226, 524
FaeI CATG 5 cut(s) 161, 257, 322, 350, 446
FaiI YATR 9 cut(s) 159, 255, 320, 348, 365, 444, 461, 502, 602
FaqI GGGAC 1 cut(s) 121
FatI CATG 5 cut(s) 157, 253, 318, 346, 442
Fnu4HI GCNGC 2 cut(s) 480, 543
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 1 cut(s) 250
Fsp4HI GCNGC 2 cut(s) 480, 543
FspBI CTAG 2 cut(s) 174, 584
GluI GCNGC 2 cut(s) 480, 543
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 125
Hin1II CATG 5 cut(s) 161, 257, 322, 350, 446
HindIII AAGCTT 1 cut(s) 327
HinfI GANTC 1 cut(s) 98
HpaII CCGG 1 cut(s) 125
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 2 cut(s) 239, 403
Hpy188III TCNNGA 2 cut(s) 174, 347
Hpy8I GTNNAC 1 cut(s) 199
HpyAV CCTTC 2 cut(s) 482, 504
HpyCH4III ACNGT 6 cut(s) 87, 303, 396, 508, 538, 629
HpyCH4V TGCA 6 cut(s) 22, 206, 341, 422, 482, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 263
HpyF3I CTNAG 1 cut(s) 40
Hsp92II CATG 5 cut(s) 161, 257, 322, 350, 446
Kzo9I GATC 2 cut(s) 528, 556
LmnI GCTCC 4 cut(s) 194, 245, 253, 476
LpnPI CCDG 7 cut(s) 87, 98, 138, 168, 268, 477, 590
Lsp1109I GCAGC 2 cut(s) 466, 554
LweI GCATC 2 cut(s) 328, 409
MaeI CTAG 2 cut(s) 174, 584
MaeIII GTNAC 2 cut(s) 448, 629
MalI GATC 2 cut(s) 530, 558
MboI GATC 2 cut(s) 528, 556
MboII GAAGA 3 cut(s) 44, 118, 632
MflI RGATCY 1 cut(s) 528
MhlI GDGCHC 2 cut(s) 250, 308
MluCI AATT 5 cut(s) 23, 72, 177, 342, 423
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 4 cut(s) 104, 233, 290, 397
MseI TTAA 1 cut(s) 533
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 1 cut(s) 125
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 2 cut(s) 254, 263
NciI CCSGG 1 cut(s) 125
NdeII GATC 2 cut(s) 528, 556
NlaIII CATG 5 cut(s) 161, 257, 322, 350, 446
NlaIV GGNNCC 1 cut(s) 190
NspI RCATGY 1 cut(s) 322
PagI TCATGA 1 cut(s) 346
PfeI GAWTC 1 cut(s) 98
PkrI GCNGC 2 cut(s) 481, 544
PpuMI RGGWCCY 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 250
Psp5II RGGWCCY 1 cut(s) 491
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 1 cut(s) 491
PspPPI RGGWCCY 1 cut(s) 491
PsuI RGATCY 1 cut(s) 528
RsaI GTAC 2 cut(s) 62, 200
RsaNI GTAC 2 cut(s) 61, 199
SacI GAGCTC 1 cut(s) 250
SaqAI TTAA 1 cut(s) 533
SatI GCNGC 2 cut(s) 480, 543
Sau3AI GATC 2 cut(s) 528, 556
Sau96I GGNCC 1 cut(s) 491
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 2 cut(s) 250, 308
SfaNI GCATC 2 cut(s) 328, 409
SfcI CTRYAG 1 cut(s) 537
SinI GGWCC 1 cut(s) 491
Sse9I AATT 5 cut(s) 23, 72, 177, 342, 423
SsiI CCGC 1 cut(s) 415
SspI AATATT 1 cut(s) 577
SspMI CTAG 2 cut(s) 174, 584
SstI GAGCTC 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 2 cut(s) 226, 524
TaaI ACNGT 6 cut(s) 87, 303, 396, 508, 538, 629
TaqI TCGA 2 cut(s) 96, 510
TaqII GACCGA 1 cut(s) 549
TasI AATT 5 cut(s) 23, 72, 177, 342, 423
TatI WGTACW 2 cut(s) 60, 198
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 1 cut(s) 533
Tru9I TTAA 1 cut(s) 533
TscAI CASTG 3 cut(s) 308, 399, 511
TseI GCWGC 2 cut(s) 479, 542
TspDTI ATGAA 4 cut(s) 17, 335, 426, 501
TspRI CASTG 3 cut(s) 308, 399, 511
VpaK11BI GGWCC 1 cut(s) 491
XbaI TCTAGA 1 cut(s) 173
XceI RCATGY 1 cut(s) 322
XspI CTAG 2 cut(s) 174, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.