RchiOBHm_Chr5g0075251

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000923
Physical Location & Seq
Reverse (-)
81053389 .. 81057285
3897 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34998

Sequence Viewer

Length: 2340 bp
ATGAAAAGATGGGATTTCTTTCTCAAAATCGTAGTGATTTTAGCATTTAAGTACAAATCTAGTCTCTATCCATTAGGTTTGGATTCTTACATTGTGTACTTGGGAACACATTCCCATGGCTTGAACCCTACGTCTGTCGAACTCGAAGCGGCAACGAATTCTCAGTACAATCTGCTTGGAACTGTTTTGGGAAGCAATGAGAGGGCCCAAGAAGCATTGTTTTACTCATATACTAGAGAGATCAATGGATTTGCTGCAGTTTTAGAAGAAGAAGAGGCTTCACAGATTGCAAAGGATCCAAGTGTTGTGTCTGTTTTTCCAAACAGAAAATTAAAACTACATACAACTCATTCATGGGAGTTCATGGGACTAGAAGACCAAAATGGAGTACCTCGTCACGGATCGATTTGGAAGAAAGCTAAGTTTGGTGAAAATACAATCATTGGAAACCTTGACACTGGTGTCTGGCCGGAATCACCGAGCTTTAGCGATGAAGGGTTTGGACCAATCCCATCCAAGTGGCGTGGAACTTGTCAACTTGACACCAAAGACAAAGTTCGTTGCAACAGGAAGCTGATCGGAGCAAGGTACTTTAACAAAGGCTTCTTGGAGTCTGCCAGGCTCAATAATGCCACCATTCCACCAGAAATTCTGGTGTCACCGCGTGACCAGGAAGGCCATGGCTCACACACCCTTTCAACAGCTGGTGGCAGTTATGTGCCGGGGGCTAATGTTTTTGGTCATGGCAATGGCACTGCTAAAGGTGGATCCCCAAAAGCTCGCGTGGCTGCTTACAAAGTATGTTGGCCAAGCTTTGGCAATACAGATGGAGGGTGTACTAATGCAGACATCATGGCCGCCTTTGATGCTGCAATCAGTGACGGTGTTGATGTTCTTTCGCTGTCCGTTGGTGGTGATTCTGTTGAGTTTTTTGAGGATGGGATCGCAATAGGTTCATTCCATGCGGCTAAGCATGGAATCGTTGTTGTTTGCTCAGCTGGCAATTCAGGGCCTTCCCAAGGGTCAGTGACTAATGTGGCACCCTGGATATTAACAGTTGGAGCTAGCACGATTGATCGCGAGTTCACTAGTTATTTATCCTTTGGAAAGGGGAAGCATCTAAAGGGAAAAAGTCTTTCTTCCAGAAGTTTGCCATATAAGAAGTTTTATCCTGTGATAAGTGGAGCCAATGCTAATGCAGCTACTTCGCTTCCTGAAGATTCCTTGCTATGTTTGAATGGAACCCTTGATCCAAAGAAGGTGAAGGGGAAGATCTTGGTCTGCCTCCGCGGGCAGAATGGGAGAACTGAAAAAGCCGTTGTAGCTGCTCTTGCCGGCGCTGTTGGAATGGTTTTGGTTAACGATGAGCAAAGTGGAGATGATCTTATGGCTGATCCTTATCAGCTCCCCGCTACACATCTGACTTACACTGATGGAAAACGTGTCTTTGAGTACCTGAATTCTACCAAGAACCCTTTTGGTCACGTTACTCCTGTCAAGACGGTAGAAGGAACAAAGCCGGCTCCATTTATGGCTTCATTCTCATCTCGTGGACCGAGTAGCATTGAGCCATCAATCCTTAAGCCTGATATCACAGCACCAGGAGTGGATATAATTGCTGCTTATACTGGAGCACCTCTCCTAACTGATTCGGACATTGGTAAGCGTCGTCCACTTTACATTACAGAATCAGGAACATCTATGTCATGCCCTCATGTATCTGGAATAGTCGGTCTTCTGAAAACACTTTACCCGTCATGGAGCCCCGCTGCTATTAAGTCTGCAATCTTGACCACAGCGAGAAGACGCGACAACAAACGGGAGTCAATTCTTGATTCAACCAAGGTCTCAGCTACACCACTTGACTATGGTGCAGGCCATGTTGATCCAAATCGCGCAGCAAACCCGGGACTTGTCTATGACGTGACTACCGATGATTACTTGAATTTCTTATGTGCTCGTGGATACAATGCATCACTTCTCAAAATATTTTCCGACAAGCCACACAAATGTTCCAACACCTCTAGCATTATTGCTGATTTAAACTATCCTACAATTGCTGTTCCTGATCTCCAAGACAAACCAATTACCATTTCTAGAAAAGTGAAGAATGTCGGTACTCCAGGCACATATGTAGCATATGTTCGTGCACCAGCCAGAGTTTCTGTTTCGGTTCAGCCTAGCAGGTTGAAATTCAAGTCCATTGGTGAAGAGAAGGAATTCAAGGTTACGCTGAAGGCAGATGGTAAGGTTCAAAATCCAGACTATGTGTTTGGAGAGTTGAAATGGTCGGATGGCAAGCACACTGTGAGGAGTCCTATTGCTGTGATGCGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

779

Amino Acids

83.87

Weight (kDa)

8.93

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 29 - 114 5.9e-17 Peptidase inhibitor I9
MORF_dom PF21864 57 - 113 1.8e-06 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 143 - 605 6.2e-41 Subtilase family
PA PF02225 408 - 481 2.2e-13 PA domain
fn3_6 PF17766 680 - 776 2.1e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 2175
AccB1I GGYRCC 1 cut(s) 1039
AccB7I CCANNNNNTGG 1 cut(s) 815
AccII CGCG 6 cut(s) 664, 783, 1080, 1290, 1809, 1896
AciI CCGC 8 cut(s) 149, 662, 858, 965, 1288, 1290, 1410, 1767
AclWI GGATC 9 cut(s) 290, 303, 409, 762, 775, 950, 1244, 1388, 1880
AcoI YGGCCR 3 cut(s) 467, 806, 855
AcsI RAATTY 6 cut(s) 157, 648, 1459, 1945, 2192, 2219
AcuI CTGAAG 2 cut(s) 1236, 2255
AdeI CACNNNGTG 2 cut(s) 665, 2308
AfaI GTAC 8 cut(s) 53, 98, 167, 390, 590, 838, 1454, 2119
AfiI CCNNNNNNNGG 3 cut(s) 398, 815, 1019
AflII CTTAAG 1 cut(s) 1580
AflIII ACRYGT 1 cut(s) 1441
AhlI ACTAGT 1 cut(s) 1088
AjiI CACGTC 1 cut(s) 1924
AjnI CCWGG 5 cut(s) 617, 669, 1043, 1600, 2122
AjuI GAANNNNNNNTTGG 2 cut(s) 2077, 2109
AloI GAACNNNNNNTCC 2 cut(s) 1234, 1266
Alw21I GWGCWC 3 cut(s) 1636, 1960, 2152
Alw26I GTCTC 2 cut(s) 68, 1852
Alw44I GTGCAC 1 cut(s) 2148
AlwI GGATC 9 cut(s) 290, 303, 409, 762, 775, 950, 1244, 1388, 1880
Ama87I CYCGRG 1 cut(s) 1906
AoxI GGCC 7 cut(s) 204, 467, 676, 806, 855, 1010, 1876
ApaI GGGCCC 1 cut(s) 208
ApaLI GTGCAC 1 cut(s) 2148
ApeKI GCWGC 8 cut(s) 254, 788, 869, 1199, 1325, 1619, 1769, 1898
ApoI RAATTY 6 cut(s) 157, 648, 1459, 1945, 2192, 2219
ArsI GACNNNNNNTTYG 2 cut(s) 2255, 2287
Asp700I GAANNNNTTC 2 cut(s) 109, 2219
AspLEI GCGC 2 cut(s) 1340, 1898
AspS9I GGNCC 5 cut(s) 204, 205, 503, 1010, 1553
AsuC2I CCSGG 3 cut(s) 723, 1907, 1908
AsuHPI GGTGA 6 cut(s) 440, 468, 651, 926, 1273, 2219
AsuNHI GCTAGC 1 cut(s) 1064
AvaI CYCGRG 1 cut(s) 1906
AvaII GGWCC 2 cut(s) 503, 1553
BaeGI GKGCMC 2 cut(s) 208, 2152
BalI TGGCCA 1 cut(s) 808
BamHI GGATCC 2 cut(s) 295, 767
BanI GGYRCC 1 cut(s) 1039
BanII GRGCYC 2 cut(s) 208, 1766
BauI CACGAG 2 cut(s) 1548, 1959
BbsI GAAGAC 3 cut(s) 381, 1727, 1810
Bbv12I GWGCWC 3 cut(s) 1636, 1960, 2152
BbvI GCAGC 8 cut(s) 241, 775, 856, 1211, 1312, 1606, 1756, 1910
BccI CCATC 8 cut(s) 3, 520, 821, 932, 1427, 1579, 2237, 2288
BceAI ACGGC 1 cut(s) 1301
BcgI CGANNNNNNTGC 2 cut(s) 1188, 1222
BciT130I CCWGG 5 cut(s) 619, 671, 1045, 1602, 2124
BciVI GTATCC 1 cut(s) 1958
BcnI CCSGG 3 cut(s) 723, 1907, 1908
BcoDI GTCTC 2 cut(s) 68, 1852
BcuI ACTAGT 1 cut(s) 1088
BfaI CTAG 9 cut(s) 60, 234, 371, 1065, 1089, 2025, 2097, 2181, 2338
BfmI CTRYAG 1 cut(s) 255
BfoI RGCGCY 1 cut(s) 1341
BfrI CTTAAG 1 cut(s) 1580
BfuAI ACCTGC 1 cut(s) 2175
BfuI GTATCC 1 cut(s) 1958
BglII AGATCT 1 cut(s) 1272
BlpI GCTNAGC 2 cut(s) 969, 994
Bme1390I CCNGG 8 cut(s) 619, 671, 723, 1045, 1602, 1907, 1908, 2124
Bme18I GGWCC 2 cut(s) 503, 1553
BmeT110I CYCGRG 1 cut(s) 1906
BmgBI CACGTC 1 cut(s) 1924
BmgT120I GGNCC 5 cut(s) 204, 205, 503, 1010, 1553
BmiI GGNNCC 8 cut(s) 206, 297, 769, 1041, 1186, 1243, 1524, 1763
BmrFI CCNGG 8 cut(s) 619, 671, 723, 1045, 1602, 1907, 1908, 2124
BmsI GCATC 4 cut(s) 856, 1126, 1982, 2319
BmtI GCTAGC 1 cut(s) 1068
BpiI GAAGAC 3 cut(s) 381, 1727, 1810
BplI GAGNNNNNCTC 2 cut(s) 1623, 1655
BpmI CTGGAG 2 cut(s) 1650, 2106
Bpu1102I GCTNAGC 2 cut(s) 969, 994
BpuMI CCSGG 3 cut(s) 723, 1907, 1908
Bsa29I ATCGAT 1 cut(s) 404
BsaBI GATNNNNATC 2 cut(s) 1398, 1890
BsaI GGTCTC 1 cut(s) 1852
BsaJI CCNNGG 8 cut(s) 115, 679, 722, 1018, 1043, 1288, 1842, 1906
Bsc4I CCNNNNNNNGG 3 cut(s) 398, 815, 1019
Bse118I RCCGGY 2 cut(s) 1334, 1519
Bse1I ACTGG 2 cut(s) 463, 1633
Bse3DI GCAATG 2 cut(s) 202, 754
Bse8I GATNNNNATC 2 cut(s) 1398, 1890
BseBI CCWGG 5 cut(s) 619, 671, 1045, 1602, 2124
BseCI ATCGAT 1 cut(s) 404
BseDI CCNNGG 8 cut(s) 115, 679, 722, 1018, 1043, 1288, 1842, 1906
BseGI GGATG 3 cut(s) 512, 943, 2299
BseJI GATNNNNATC 2 cut(s) 1398, 1890
BseLI CCNNNNNNNGG 3 cut(s) 398, 815, 1019
BseMI GCAATG 2 cut(s) 202, 754
BseMII CTCAG 3 cut(s) 176, 1008, 1863
BseNI ACTGG 2 cut(s) 463, 1633
BseRI GAGGAG 1 cut(s) 2326
BseSI GKGCMC 2 cut(s) 208, 2152
BseXI GCAGC 8 cut(s) 241, 775, 856, 1211, 1312, 1606, 1756, 1910
BsgI GTGCAG 1 cut(s) 1893
Bsh1236I CGCG 6 cut(s) 664, 783, 1080, 1290, 1809, 1896
BshFI GGCC 7 cut(s) 206, 469, 678, 808, 857, 1012, 1878
BshNI GGYRCC 1 cut(s) 1039
BshVI ATCGAT 1 cut(s) 404
BsiHKAI GWGCWC 3 cut(s) 1636, 1960, 2152
BsiHKCI CYCGRG 1 cut(s) 1906
BsiSI CCGG 5 cut(s) 470, 722, 1335, 1520, 1907
BslFI GGGAC 2 cut(s) 381, 1923
BslI CCNNNNNNNGG 3 cut(s) 398, 815, 1019
BsmAI GTCTC 2 cut(s) 68, 1852
BsmFI GGGAC 2 cut(s) 381, 1923
BsnI GGCC 7 cut(s) 206, 469, 678, 808, 857, 1012, 1878
Bso31I GGTCTC 1 cut(s) 1852
BsoBI CYCGRG 1 cut(s) 1906
Bsp120I GGGCCC 1 cut(s) 204
Bsp1286I GDGCHC 5 cut(s) 208, 1636, 1766, 1960, 2152
Bsp1720I GCTNAGC 2 cut(s) 969, 994
Bsp19I CCATGG 2 cut(s) 115, 679
Bsp68I TCGCGA 1 cut(s) 1080
BspACI CCGC 8 cut(s) 149, 662, 858, 965, 1288, 1290, 1410, 1767
BspANI GGCC 7 cut(s) 206, 469, 678, 808, 857, 1012, 1878
BspCNI CTCAG 3 cut(s) 175, 1007, 1862
BspDI ATCGAT 1 cut(s) 404
BspFNI CGCG 6 cut(s) 664, 783, 1080, 1290, 1809, 1896
BspLI GGNNCC 8 cut(s) 206, 297, 769, 1041, 1186, 1243, 1524, 1763
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 2175
BspOI GCTAGC 1 cut(s) 1068
BspPI GGATC 9 cut(s) 290, 303, 409, 762, 775, 950, 1244, 1388, 1880
BspT107I GGYRCC 1 cut(s) 1039
BspTI CTTAAG 1 cut(s) 1580
BspTNI GGTCTC 1 cut(s) 1852
BsrDI GCAATG 2 cut(s) 202, 754
BsrFI RCCGGY 2 cut(s) 1334, 1519
BsrI ACTGG 2 cut(s) 463, 1633
BssAI RCCGGY 2 cut(s) 1334, 1519
BssECI CCNNGG 8 cut(s) 115, 679, 722, 1018, 1043, 1288, 1842, 1906
BssSI CACGAG 2 cut(s) 1548, 1959
BssT1I CCWWGG 4 cut(s) 115, 679, 1018, 1842
Bst2BI CACGAG 2 cut(s) 1548, 1959
Bst2UI CCWGG 5 cut(s) 619, 671, 1045, 1602, 2124
Bst4CI ACNGT 5 cut(s) 184, 884, 1057, 1504, 2308
Bst6I CTCTTC 2 cut(s) 267, 2205
BstAFI CTTAAG 1 cut(s) 1580
BstC8I GCNNGC 8 cut(s) 781, 1000, 1066, 1292, 1336, 1521, 1876, 2300
BstDEI CTNAG 5 cut(s) 162, 420, 969, 994, 1849
BstDSI CCRYGG 3 cut(s) 115, 679, 1288
BstENI CCTNNNNNAGG 1 cut(s) 1017
BstF5I GGATG 3 cut(s) 512, 943, 2299
BstFNI CGCG 6 cut(s) 664, 783, 1080, 1290, 1809, 1896
BstH2I RGCGCY 1 cut(s) 1341
BstHHI GCGC 2 cut(s) 1340, 1898
BstMAI GTCTC 2 cut(s) 68, 1852
BstMWI GCNNNNNNNGC 7 cut(s) 212, 785, 866, 999, 1199, 1322, 1331
BstNI CCWGG 5 cut(s) 619, 671, 1045, 1602, 2124
BstSCI CCNGG 8 cut(s) 617, 669, 721, 1043, 1600, 1905, 1906, 2122
BstSFI CTRYAG 1 cut(s) 255
BstSLI GKGCMC 2 cut(s) 208, 2152
BstUI CGCG 6 cut(s) 664, 783, 1080, 1290, 1809, 1896
BstV1I GCAGC 8 cut(s) 241, 775, 856, 1211, 1312, 1606, 1756, 1910
BstV2I GAAGAC 3 cut(s) 381, 1727, 1810
BstX2I RGATCY 3 cut(s) 295, 767, 1272
BstXI CCANNNNNNTGG 1 cut(s) 519
BstYI RGATCY 3 cut(s) 295, 767, 1272
Bsu15I ATCGAT 1 cut(s) 404
BsuI GTATCC 1 cut(s) 1958
BsuRI GGCC 7 cut(s) 206, 469, 678, 808, 857, 1012, 1878
BsuTUI ATCGAT 1 cut(s) 404
BtgI CCRYGG 3 cut(s) 115, 679, 1288
BtgZI GCGATG 1 cut(s) 504
BtrI CACGTC 1 cut(s) 1924
BtsCI GGATG 3 cut(s) 512, 943, 2299
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 6 cut(s) 456, 753, 883, 1032, 1428, 2304
BtuMI TCGCGA 1 cut(s) 1080
BveI ACCTGC 1 cut(s) 2175
Cac8I GCNNGC 8 cut(s) 781, 1000, 1066, 1292, 1336, 1521, 1876, 2300
CfoI GCGC 2 cut(s) 1340, 1898
Cfr10I RCCGGY 2 cut(s) 1334, 1519
Cfr13I GGNCC 5 cut(s) 204, 205, 503, 1010, 1553
Cfr42I CCGCGG 1 cut(s) 1291
Cfr9I CCCGGG 1 cut(s) 1906
ClaI ATCGAT 1 cut(s) 404
CseI GACGC 3 cut(s) 1655, 1815, 2321
Csp6I GTAC 8 cut(s) 52, 97, 166, 389, 589, 837, 1453, 2118
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviQI GTAC 8 cut(s) 52, 97, 166, 389, 589, 837, 1453, 2118
DdeI CTNAG 5 cut(s) 162, 420, 969, 994, 1849
DraI TTTAAA 1 cut(s) 2043
DraIII CACNNNGTG 2 cut(s) 665, 2308
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 3 cut(s) 467, 806, 855
Eam1104I CTCTTC 2 cut(s) 267, 2205
EarI CTCTTC 2 cut(s) 267, 2205
Eco130I CCWWGG 4 cut(s) 115, 679, 1018, 1842
Eco24I GRGCYC 2 cut(s) 208, 1766
Eco31I GGTCTC 1 cut(s) 1852
Eco32I GATATC 1 cut(s) 1591
Eco47I GGWCC 2 cut(s) 503, 1553
Eco57I CTGAAG 2 cut(s) 1236, 2255
Eco88I CYCGRG 1 cut(s) 1906
EcoNI CCTNNNNNAGG 1 cut(s) 1017
EcoO109I RGGNCCY 2 cut(s) 204, 1010
EcoRI GAATTC 3 cut(s) 157, 1459, 2219
EcoRII CCWGG 5 cut(s) 617, 669, 1043, 1600, 2122
EcoRV GATATC 1 cut(s) 1591
EcoT14I CCWWGG 4 cut(s) 115, 679, 1018, 1842
EcoT22I ATGCAT 1 cut(s) 1975
EcoT38I GRGCYC 2 cut(s) 208, 1766
ErhI CCWWGG 4 cut(s) 115, 679, 1018, 1842
FalI AAGNNNNNCTT 2 cut(s) 1123, 1155
FaqI GGGAC 2 cut(s) 381, 1923
FauI CCCGC 3 cut(s) 1283, 1417, 1774
FauNDI CATATG 2 cut(s) 2131, 2140
FokI GGATG 3 cut(s) 499, 950, 2306
FriOI GRGCYC 2 cut(s) 208, 1766
FspBI CTAG 9 cut(s) 60, 234, 371, 1065, 1089, 2025, 2097, 2181, 2338
GlaI GCGC 2 cut(s) 1339, 1897
GsuI CTGGAG 2 cut(s) 1650, 2106
HaeII RGCGCY 1 cut(s) 1341
HaeIII GGCC 7 cut(s) 206, 469, 678, 808, 857, 1012, 1878
HapII CCGG 5 cut(s) 470, 722, 1335, 1520, 1907
HgaI GACGC 3 cut(s) 1655, 1815, 2321
HhaI GCGC 2 cut(s) 1340, 1898
Hin6I GCGC 2 cut(s) 1338, 1896
HinP1I GCGC 2 cut(s) 1338, 1896
HincII GTYRAC 2 cut(s) 536, 1360
HindII GTYRAC 2 cut(s) 536, 1360
HindIII AAGCTT 1 cut(s) 811
HpaI GTTAAC 1 cut(s) 1360
HpaII CCGG 5 cut(s) 470, 722, 1335, 1520, 1907
HphI GGTGA 6 cut(s) 440, 468, 651, 926, 1273, 2219
Hpy166II GTNNAC 8 cut(s) 97, 536, 837, 1086, 1360, 1553, 1673, 2150
Hpy188I TCNGA 6 cut(s) 581, 1422, 1654, 1740, 1996, 2293
Hpy8I GTNNAC 8 cut(s) 97, 536, 837, 1086, 1360, 1553, 1673, 2150
Hpy99I CGWCG 1 cut(s) 1671
HpyAV CCTTC 8 cut(s) 488, 668, 1023, 1252, 1258, 1502, 2209, 2230
HpyCH4III ACNGT 5 cut(s) 184, 884, 1057, 1504, 2308
HpyCH4IV ACGT 4 cut(s) 131, 1441, 1485, 1923
HpyF10VI GCNNNNNNNGC 7 cut(s) 212, 785, 866, 999, 1199, 1322, 1331
HpyF3I CTNAG 5 cut(s) 162, 420, 969, 994, 1849
HpySE526I ACGT 4 cut(s) 131, 1441, 1485, 1923
HspAI GCGC 2 cut(s) 1338, 1896
KroI GCCGGC 2 cut(s) 1334, 1519
KroNI GCCGGC 2 cut(s) 1336, 1521
KspAI GTTAAC 1 cut(s) 1360
KspI CCGCGG 1 cut(s) 1291
LmnI GCTCC 7 cut(s) 581, 1061, 1184, 1410, 1528, 1631, 1761
Lsp1109I GCAGC 8 cut(s) 241, 775, 856, 1211, 1312, 1606, 1756, 1910
LweI GCATC 4 cut(s) 856, 1126, 1982, 2319
MaeI CTAG 9 cut(s) 60, 234, 371, 1065, 1089, 2025, 2097, 2181, 2338
MaeII ACGT 4 cut(s) 131, 1441, 1485, 1923
MfeI CAATTG 1 cut(s) 2055
MflI RGATCY 3 cut(s) 295, 767, 1272
MhlI GDGCHC 5 cut(s) 208, 1636, 1766, 1960, 2152
MlsI TGGCCA 1 cut(s) 808
MluNI TGGCCA 1 cut(s) 808
MlyI GAGTC 3 cut(s) 620, 1832, 2323
MmeI TCCRAC 5 cut(s) 1039, 1324, 2019, 2040, 2271
Mox20I TGGCCA 1 cut(s) 808
Mph1103I ATGCAT 1 cut(s) 1975
MroNI GCCGGC 2 cut(s) 1334, 1519
MroXI GAANNNNTTC 2 cut(s) 109, 2219
MscI TGGCCA 1 cut(s) 808
MseI TTAA 8 cut(s) 48, 332, 594, 1052, 1359, 1581, 1776, 2042
MslI CAYNNNNRTG 4 cut(s) 114, 517, 747, 2008
Msp20I TGGCCA 1 cut(s) 808
MspA1I CMGCKG 4 cut(s) 704, 998, 1290, 1769
MspCI CTTAAG 1 cut(s) 1580
MspI CCGG 5 cut(s) 470, 722, 1335, 1520, 1907
MspR9I CCNGG 8 cut(s) 619, 671, 723, 1045, 1602, 1907, 1908, 2124
MunI CAATTG 1 cut(s) 2055
MvaI CCWGG 5 cut(s) 619, 671, 1045, 1602, 2124
MvnI CGCG 6 cut(s) 664, 783, 1080, 1290, 1809, 1896
MwoI GCNNNNNNNGC 7 cut(s) 212, 785, 866, 999, 1199, 1322, 1331
NaeI GCCGGC 2 cut(s) 1336, 1521
NciI CCSGG 3 cut(s) 723, 1907, 1908
NcoI CCATGG 2 cut(s) 115, 679
NdeI CATATG 2 cut(s) 2131, 2140
NgoMIV GCCGGC 2 cut(s) 1334, 1519
NheI GCTAGC 1 cut(s) 1064
NlaIV GGNNCC 8 cut(s) 206, 297, 769, 1041, 1186, 1243, 1524, 1763
NmuCI GTSAC 8 cut(s) 395, 657, 665, 878, 1027, 1481, 1924, 2333
NruI TCGCGA 1 cut(s) 1080
NsiI ATGCAT 1 cut(s) 1975
PcsI WCGNNNNNNNCGW 1 cut(s) 1929
PdiI GCCGGC 2 cut(s) 1336, 1521
PdmI GAANNNNTTC 2 cut(s) 109, 2219
PfeI GAWTC 8 cut(s) 83, 473, 917, 978, 1220, 1649, 1688, 1835
PflMI CCANNNNNTGG 1 cut(s) 815
PleI GAGTC 3 cut(s) 619, 1831, 2322
PpsI GAGTC 3 cut(s) 619, 1831, 2322
Psp6I CCWGG 5 cut(s) 617, 669, 1043, 1600, 2122
PspGI CCWGG 5 cut(s) 617, 669, 1043, 1600, 2122
PspN4I GGNNCC 8 cut(s) 206, 297, 769, 1041, 1186, 1243, 1524, 1763
PspOMI GGGCCC 1 cut(s) 204
PspPI GGNCC 5 cut(s) 204, 205, 503, 1010, 1553
PsrI GAACNNNNNNTAC 2 cut(s) 2127, 2159
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 3 cut(s) 295, 767, 1272
PvuII CAGCTG 2 cut(s) 704, 998
RruI TCGCGA 1 cut(s) 1080
RsaI GTAC 8 cut(s) 53, 98, 167, 390, 590, 838, 1454, 2119
RsaNI GTAC 8 cut(s) 52, 97, 166, 389, 589, 837, 1453, 2118
RseI CAYNNNNRTG 4 cut(s) 114, 517, 747, 2008
SacII CCGCGG 1 cut(s) 1291
SaqAI TTAA 8 cut(s) 48, 332, 594, 1052, 1359, 1581, 1776, 2042
Sau96I GGNCC 5 cut(s) 204, 205, 503, 1010, 1553
SchI GAGTC 3 cut(s) 620, 1832, 2323
ScrFI CCNGG 8 cut(s) 619, 671, 723, 1045, 1602, 1907, 1908, 2124
SduI GDGCHC 5 cut(s) 208, 1636, 1766, 1960, 2152
SfaNI GCATC 4 cut(s) 856, 1126, 1982, 2319
SfcI CTRYAG 1 cut(s) 255
Sfr303I CCGCGG 1 cut(s) 1291
SgrBI CCGCGG 1 cut(s) 1291
SinI GGWCC 2 cut(s) 503, 1553
SmaI CCCGGG 1 cut(s) 1908
SmiMI CAYNNNNRTG 4 cut(s) 114, 517, 747, 2008
SmlI CTYRAG 1 cut(s) 1580
SmoI CTYRAG 1 cut(s) 1580
SpeI ACTAGT 1 cut(s) 1088
SsiI CCGC 8 cut(s) 149, 662, 858, 965, 1288, 1290, 1410, 1767
SspI AATATT 1 cut(s) 1989
SspMI CTAG 9 cut(s) 60, 234, 371, 1065, 1089, 2025, 2097, 2181, 2338
StyD4I CCNGG 8 cut(s) 617, 669, 721, 1043, 1600, 1905, 1906, 2122
StyI CCWWGG 4 cut(s) 115, 679, 1018, 1842
TaaI ACNGT 5 cut(s) 184, 884, 1057, 1504, 2308
TaiI ACGT 4 cut(s) 134, 1444, 1488, 1926
TaqI TCGA 3 cut(s) 138, 144, 404
TaqII GACCGA 2 cut(s) 1570, 1721
TatI WGTACW 4 cut(s) 51, 96, 165, 836
TauI GCSGC 3 cut(s) 152, 860, 968
TfiI GAWTC 8 cut(s) 83, 473, 917, 978, 1220, 1649, 1688, 1835
Tru1I TTAA 8 cut(s) 48, 332, 594, 1052, 1359, 1581, 1776, 2042
Tru9I TTAA 8 cut(s) 48, 332, 594, 1052, 1359, 1581, 1776, 2042
TscAI CASTG 6 cut(s) 463, 760, 883, 1032, 1435, 2311
TseFI GTSAC 8 cut(s) 395, 657, 665, 878, 1027, 1481, 1924, 2333
TseI GCWGC 8 cut(s) 254, 788, 869, 1199, 1325, 1619, 1769, 1898
Tsp45I GTSAC 8 cut(s) 395, 657, 665, 878, 1027, 1481, 1924, 2333
TspDTI ATGAA 6 cut(s) 17, 342, 352, 507, 945, 1527
TspGWI ACGGA 2 cut(s) 414, 895
TspMI CCCGGG 1 cut(s) 1906
TspRI CASTG 6 cut(s) 463, 760, 883, 1032, 1435, 2311
Van91I CCANNNNNTGG 1 cut(s) 815
Vha464I CTTAAG 1 cut(s) 1580
VneI GTGCAC 1 cut(s) 2148
VpaK11BI GGWCC 2 cut(s) 503, 1553
XagI CCTNNNNNAGG 1 cut(s) 1017
XapI RAATTY 6 cut(s) 157, 648, 1459, 1945, 2192, 2219
XbaI TCTAGA 1 cut(s) 2096
XcmI CCANNNNNNNNNTGG 1 cut(s) 677
XmaI CCCGGG 1 cut(s) 1906
XmnI GAANNNNTTC 2 cut(s) 109, 2219
XspI CTAG 9 cut(s) 60, 234, 371, 1065, 1089, 2025, 2097, 2181, 2338
Zsp2I ATGCAT 1 cut(s) 1975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.