pycom11g03370

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000923
Physical Location & Seq
Forward (+)
2627596 .. 2628568
973 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03370.3

Sequence Viewer

Length: 810 bp
ATGGCTTCATTCTCTTCGAGGGGACCTAACGCCATTGAGCAGTCAATCCTTAAGCCTGATATCACAGCGCCAGGAGTGAGTATAATTGCTGCTTATACTCAAGCAACCGGGCCAACTGGTCAGAAGTTCGATAACCGGCGTGTATCTTTCAATACACAATCCGGAACTTCCATGTCGTGCCCTCATGTATCTGGAATTGTCGGTCTTTTGAAAACCCTTCACCCGACATGGAGCCCTGCAGCTATTAAATCTGCAATCATGACCACCGCAAGAAAACGAGATAACAGCAAGGGCACAATGCTGGACTCATCCAAGGCCAGAGCAACACCGTTTGCTTATGGTGCAGGACATGTTCAACCAAACCGTGCAATGGACCCTGGACTTGTTTATGACCTAACTACCGATGATTACTTAAACTTCTTATGCGCTCGTGGCTACAATGCAACGCTGCTCAAAGTGTTTGCTAAAGAGCCACACAAATGTTCCAAGGCATATAGTCTTTCTGATTTTAACTATCCTTCTATAACAGTTCCCAATCTCCGTGACACGCCAGTGACCGTGACTAGAAGGGTTAAGAACGTCGGCTCTCCAGGCACATATGTAGTTCGTATTAAGGAACCGGTGGGAGTTTCTGTTACAGTTAAGCCTAGTACCTTGCAATTCAAGAGCAGTGGCGAAGAGAAGAAGTTCAAGGTTGTTCTGAAGCCTAAGGTTCAAGGGCCTGAAGACTACGTATTTGGAGAGTTAAATTGGATGGATGGGAAGCACAATGTCAGGAGTCCTATTGTTGTCATGCACTATCAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

270

Amino Acids

29.41

Weight (kDa)

9.82

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 161
AciI CCGC 1 cut(s) 267
AcuI CTGAAG 2 cut(s) 722, 744
AfaI GTAC 1 cut(s) 652
AfiI CCNNNNNNNGG 1 cut(s) 370
AflII CTTAAG 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 349
AgeI ACCGGT 1 cut(s) 619
AgsI TTSAA 6 cut(s) 151, 211, 356, 664, 691, 716
AjnI CCWGG 3 cut(s) 70, 376, 589
AleI CACNNNNGTG 1 cut(s) 551
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 161
AoxI GGCC 3 cut(s) 110, 315, 719
ApeKI GCWGC 3 cut(s) 89, 239, 448
ArsI GACNNNNNNTTYG 2 cut(s) 719, 751
AsiGI ACCGGT 1 cut(s) 619
Asp700I GAANNNNTTC 1 cut(s) 686
AspLEI GCGC 2 cut(s) 70, 428
AspS9I GGNCC 4 cut(s) 23, 110, 373, 719
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 212
AvaII GGWCC 2 cut(s) 23, 373
AxyI CCTNAGG 1 cut(s) 708
BaeGI GKGCMC 2 cut(s) 182, 296
BanII GRGCYC 1 cut(s) 236
BauI CACGAG 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 732
BbvI GCAGC 3 cut(s) 76, 251, 435
BccI CCATC 2 cut(s) 748, 752
BciT130I CCWGG 3 cut(s) 72, 378, 591
BcnI CCSGG 1 cut(s) 109
BfaI CTAG 2 cut(s) 564, 648
BfmI CTRYAG 1 cut(s) 237
BfoI RGCGCY 1 cut(s) 71
BfrI CTTAAG 1 cut(s) 50
BisI GCNGC 3 cut(s) 90, 240, 449
BlsI GCNGC 3 cut(s) 91, 241, 450
Bme1390I CCNGG 4 cut(s) 72, 109, 378, 591
Bme18I GGWCC 2 cut(s) 23, 373
BmgT120I GGNCC 4 cut(s) 23, 110, 373, 719
BmiI GGNNCC 4 cut(s) 24, 233, 375, 618
BmrFI CCNGG 4 cut(s) 72, 109, 378, 591
BpiI GAAGAC 1 cut(s) 732
BpmI CTGGAG 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 109
BsaAI YACGTR 1 cut(s) 733
BsaJI CCNNGG 3 cut(s) 312, 376, 486
BsaWI WCCGGW 2 cut(s) 161, 619
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 370
Bse118I RCCGGY 2 cut(s) 135, 619
Bse1I ACTGG 2 cut(s) 121, 551
Bse21I CCTNAGG 1 cut(s) 708
Bse3DI GCAATG 1 cut(s) 375
BseAI TCCGGA 1 cut(s) 161
BseBI CCWGG 3 cut(s) 72, 378, 591
BseDI CCNNGG 3 cut(s) 312, 376, 486
BseGI GGATG 3 cut(s) 308, 759, 763
BseLI CCNNNNNNNGG 1 cut(s) 370
BseMI GCAATG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 121, 551
BseSI GKGCMC 2 cut(s) 182, 296
BseXI GCAGC 3 cut(s) 76, 251, 435
BsgI GTGCAG 1 cut(s) 363
BshFI GGCC 3 cut(s) 112, 317, 721
BshTI ACCGGT 1 cut(s) 619
BsiSI CCGG 4 cut(s) 108, 136, 162, 620
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 3 cut(s) 112, 317, 721
Bsp1286I GDGCHC 3 cut(s) 182, 236, 296
Bsp13I TCCGGA 1 cut(s) 161
BspACI CCGC 1 cut(s) 267
BspANI GGCC 3 cut(s) 112, 317, 721
BspEI TCCGGA 1 cut(s) 161
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 4 cut(s) 24, 233, 375, 618
BspMAI CTGCAG 1 cut(s) 241
BspTI CTTAAG 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 375
BsrFI RCCGGY 2 cut(s) 135, 619
BsrI ACTGG 2 cut(s) 121, 551
BssAI RCCGGY 2 cut(s) 135, 619
BssECI CCNNGG 3 cut(s) 312, 376, 486
BssSI CACGAG 1 cut(s) 429
BssT1I CCWWGG 2 cut(s) 312, 486
Bst2BI CACGAG 1 cut(s) 429
Bst2UI CCWGG 3 cut(s) 72, 378, 591
Bst4CI ACNGT 5 cut(s) 330, 365, 529, 559, 640
Bst6I CTCTTC 2 cut(s) 19, 672
BstAFI CTTAAG 1 cut(s) 50
BstBAI YACGTR 1 cut(s) 733
BstDEI CTNAG 1 cut(s) 708
BstF5I GGATG 3 cut(s) 308, 759, 763
BstH2I RGCGCY 1 cut(s) 71
BstHHI GCGC 2 cut(s) 70, 428
BstMWI GCNNNNNNNGC 3 cut(s) 341, 432, 591
BstNI CCWGG 3 cut(s) 72, 378, 591
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 4 cut(s) 70, 107, 376, 589
BstSFI CTRYAG 1 cut(s) 237
BstSLI GKGCMC 2 cut(s) 182, 296
BstSNI TACGTA 1 cut(s) 733
BstV1I GCAGC 3 cut(s) 76, 251, 435
BstV2I GAAGAC 1 cut(s) 732
Bsu36I CCTNAGG 1 cut(s) 708
BsuRI GGCC 3 cut(s) 112, 317, 721
BtsCI GGATG 3 cut(s) 308, 759, 763
BtsI GCAGTG 1 cut(s) 676
BtsIMutI CAGTG 2 cut(s) 558, 676
CciI TCATGA 1 cut(s) 258
CfoI GCGC 2 cut(s) 70, 428
Cfr10I RCCGGY 2 cut(s) 135, 619
Cfr13I GGNCC 4 cut(s) 23, 110, 373, 719
Csp6I GTAC 1 cut(s) 651
CspAI ACCGGT 1 cut(s) 619
CspCI CAANNNNNGTGG 2 cut(s) 652, 687
CviAII CATG 6 cut(s) 172, 185, 228, 259, 350, 793
CviQI GTAC 1 cut(s) 651
DdeI CTNAG 1 cut(s) 708
Eam1104I CTCTTC 2 cut(s) 19, 672
EarI CTCTTC 2 cut(s) 19, 672
Eco105I TACGTA 1 cut(s) 733
Eco130I CCWWGG 2 cut(s) 312, 486
Eco24I GRGCYC 1 cut(s) 236
Eco32I GATATC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 23, 373
Eco57I CTGAAG 2 cut(s) 722, 744
Eco81I CCTNAGG 1 cut(s) 708
EcoO109I RGGNCCY 2 cut(s) 23, 719
EcoRII CCWGG 3 cut(s) 70, 376, 589
EcoRV GATATC 1 cut(s) 61
EcoT14I CCWWGG 2 cut(s) 312, 486
EcoT38I GRGCYC 1 cut(s) 236
ErhI CCWWGG 2 cut(s) 312, 486
FaeI CATG 6 cut(s) 175, 188, 231, 262, 353, 796
FaqI GGGAC 1 cut(s) 36
FatI CATG 6 cut(s) 171, 184, 227, 258, 349, 792
FauNDI CATATG 1 cut(s) 598
Fnu4HI GCNGC 3 cut(s) 90, 240, 449
FokI GGATG 3 cut(s) 295, 766, 770
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 3 cut(s) 90, 240, 449
FspBI CTAG 2 cut(s) 564, 648
GlaI GCGC 2 cut(s) 69, 427
GluI GCNGC 3 cut(s) 90, 240, 449
GsuI CTGGAG 1 cut(s) 573
HaeII RGCGCY 1 cut(s) 71
HaeIII GGCC 3 cut(s) 112, 317, 721
HapII CCGG 4 cut(s) 108, 136, 162, 620
HhaI GCGC 2 cut(s) 70, 428
Hin1II CATG 6 cut(s) 175, 188, 231, 262, 353, 796
Hin6I GCGC 2 cut(s) 68, 426
HinP1I GCGC 2 cut(s) 68, 426
HinfI GANTC 2 cut(s) 305, 778
HpaII CCGG 4 cut(s) 108, 136, 162, 620
HphI GGTGA 1 cut(s) 212
Hpy188I TCNGA 4 cut(s) 123, 505, 702, 804
Hpy188III TCNNGA 5 cut(s) 162, 192, 259, 664, 775
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 3 cut(s) 227, 528, 561
HpyCH4III ACNGT 5 cut(s) 330, 365, 529, 559, 640
HpyCH4IV ACGT 2 cut(s) 579, 732
HpyCH4V TGCA 7 cut(s) 239, 254, 344, 368, 443, 658, 796
HpyF10VI GCNNNNNNNGC 3 cut(s) 341, 432, 591
HpyF3I CTNAG 1 cut(s) 708
HpySE526I ACGT 2 cut(s) 579, 732
Hsp92II CATG 6 cut(s) 175, 188, 231, 262, 353, 796
HspAI GCGC 2 cut(s) 68, 426
Kpn2I TCCGGA 1 cut(s) 161
LmnI GCTCC 1 cut(s) 231
Lsp1109I GCAGC 3 cut(s) 76, 251, 435
MaeI CTAG 2 cut(s) 564, 648
MaeII ACGT 2 cut(s) 579, 732
MaeIII GTNAC 4 cut(s) 542, 553, 559, 634
MboII GAAGA 4 cut(s) 6, 689, 694, 737
MhlI GDGCHC 3 cut(s) 182, 236, 296
MluCI AATT 4 cut(s) 84, 195, 659, 748
MlyI GAGTC 2 cut(s) 299, 787
MnlI CCTC 2 cut(s) 12, 192
MroI TCCGGA 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 686
MseI TTAA 8 cut(s) 51, 246, 413, 510, 573, 612, 642, 746
MslI CAYNNNNRTG 2 cut(s) 478, 551
MspCI CTTAAG 1 cut(s) 50
MspI CCGG 4 cut(s) 108, 136, 162, 620
MspR9I CCNGG 4 cut(s) 72, 109, 378, 591
MvaI CCWGG 3 cut(s) 72, 378, 591
MwoI GCNNNNNNNGC 3 cut(s) 341, 432, 591
NciI CCSGG 1 cut(s) 109
NdeI CATATG 1 cut(s) 598
NlaIII CATG 6 cut(s) 175, 188, 231, 262, 353, 796
NlaIV GGNNCC 4 cut(s) 24, 233, 375, 618
NmuCI GTSAC 3 cut(s) 542, 553, 559
NspI RCATGY 1 cut(s) 353
OliI CACNNNNGTG 1 cut(s) 551
PagI TCATGA 1 cut(s) 258
PciI ACATGT 1 cut(s) 349
PdmI GAANNNNTTC 1 cut(s) 686
PinAI ACCGGT 1 cut(s) 619
PkrI GCNGC 3 cut(s) 91, 241, 450
PleI GAGTC 2 cut(s) 299, 786
PpsI GAGTC 2 cut(s) 299, 786
Ppu21I YACGTR 1 cut(s) 733
PpuMI RGGWCCY 1 cut(s) 23
PscI ACATGT 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 23
Psp6I CCWGG 3 cut(s) 70, 376, 589
PspGI CCWGG 3 cut(s) 70, 376, 589
PspN4I GGNNCC 4 cut(s) 24, 233, 375, 618
PspPI GGNCC 4 cut(s) 23, 110, 373, 719
PspPPI RGGWCCY 1 cut(s) 23
PstI CTGCAG 1 cut(s) 241
RsaI GTAC 1 cut(s) 652
RsaNI GTAC 1 cut(s) 651
RseI CAYNNNNRTG 2 cut(s) 478, 551
SaqAI TTAA 8 cut(s) 51, 246, 413, 510, 573, 612, 642, 746
SatI GCNGC 3 cut(s) 90, 240, 449
Sau96I GGNCC 4 cut(s) 23, 110, 373, 719
SchI GAGTC 2 cut(s) 299, 787
ScrFI CCNGG 4 cut(s) 72, 109, 378, 591
SduI GDGCHC 3 cut(s) 182, 236, 296
SetI ASST 8 cut(s) 28, 244, 396, 582, 656, 696, 714, 735
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 2 cut(s) 23, 373
SmiMI CAYNNNNRTG 2 cut(s) 478, 551
SmlI CTYRAG 2 cut(s) 50, 99
SmoI CTYRAG 2 cut(s) 50, 99
SnaBI TACGTA 1 cut(s) 733
Sse9I AATT 4 cut(s) 84, 195, 659, 748
SsiI CCGC 1 cut(s) 267
SspMI CTAG 2 cut(s) 564, 648
StyD4I CCNGG 4 cut(s) 70, 107, 376, 589
StyI CCWWGG 2 cut(s) 312, 486
TaaI ACNGT 5 cut(s) 330, 365, 529, 559, 640
TaiI ACGT 2 cut(s) 582, 735
TaqI TCGA 2 cut(s) 17, 129
TaqII GACCGA 1 cut(s) 191
TasI AATT 4 cut(s) 84, 195, 659, 748
Tru1I TTAA 8 cut(s) 51, 246, 413, 510, 573, 612, 642, 746
Tru9I TTAA 8 cut(s) 51, 246, 413, 510, 573, 612, 642, 746
TscAI CASTG 2 cut(s) 558, 676
TseFI GTSAC 3 cut(s) 542, 553, 559
TseI GCWGC 3 cut(s) 89, 239, 448
Tsp45I GTSAC 3 cut(s) 542, 553, 559
TspGWI ACGGA 1 cut(s) 530
TspRI CASTG 2 cut(s) 558, 676
Vha464I CTTAAG 1 cut(s) 50
VpaK11BI GGWCC 2 cut(s) 23, 373
XceI RCATGY 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 2 cut(s) 564, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.