Rh5AG494600

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000923
Physical Location & Seq
Reverse (-)
84137988 .. 84143093
5106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG494600.1

Sequence Viewer

Length: 2154 bp
ATGGATACGCCGGCCAATGCAGCCAAACAGTCTTACATTGTGTACTTGGGAACACATTCCCATGGCTTGAACCCTACGTCTGTCGAACTCGAAGCGGCAACGAATTCTCAGTACAATCTGCTTGGAACTGTTTTGGGAAGCAATGAGAGGGCCCAAGAAGCATTGTTTTACTCCTATACTAGAGAGATCAATGGATTTGCTGCAGTTTTAGAAGAAGAAGAGGCTTCACAGATTGCAAAGGATCCAAGTGTTGTGTCTGTTTTTCCAAACAGAAAATTAAAACTACATACAACTCATTCATGGGAGTTCATGGGACTAGAAGACCAAAATGGAGTACCTCGTCACGGATCGATTTGGAAGAAAGCTAAGTTTGGTGAAAATACAATCATTGGAAACCTTGACACTGGTGTCTGGCCAGAATCACCGAGCTTTAGCGATGAAGGGTTTGGACCAATCCCATCCAAGTGGCGTGGCACTTGTCAGCTTGACACCAAAGACAAAGTTCGTTGCAACAGGAAGCTGATCGGAGCAAGGTACTTTAACAAAGGCTTCTTGGAGTCTGCCAGGCTCAATAATGCCACCATTCCACCAGAGATTCTGGTGTCACCACGTGACCAGGAAGGCCATGGCTCACACACCCTTTCAACAGCTGGTGGTATTTATGTGCCGGGGGCTAATGTTTTTGGTCATGGCAATGGCACTGCTAAAGGTGGATCCCCAAAAGCTCGCGTGGCTGCTTACAAAGTATGTTGGCCAAGCTTTGGCAATACAGAAGGAGGGTGTACTAATGCAGACATCATGGCCGCCTTTGATGCTGCAATCAGTGACGGTGTTGATGTTCTTTCGCTGTCCATTGGTGGTGATTCTGTTGAGTTTTTTGAGGATGGGATCGCAATAGGGTCATTCCATGCGGCTAAGCATGGAATCGTTGTTGTTTGCTCAGCTGGCAATTCAGGGCCTACCCAAGGGACAGTGACTAATGTGGCACCCTGGATATTAACAGTTGGAGCTAGCACGATTGATCGTGAGTTCACTAGTTATTTATCCTTTGGAAAGGGGAAGCATCTAAAGGGAAAAAGTCTTTCTTCCAGAAGTTTGCCATCTAAGAAGTTTTATCCTGTGATAAGTGGAGCCAACGCTAATGCAGCTACTTCGCCTCCTGAAGATTCCTTGCTATGTTTGAATGGAACCCTTGATCCAAAGAAGGTTAAGGGGAAAATCTTGGTCTGCCTCCGCGGGCAGAATGGGAGAACTGAAAAAGCCAATATAGCTGCTCTTGCCGGCGCTGTTGGAATGGTTTTGGTTAACGATGAGGAAAGTGGAGATGATCTTATGGCTGATCCTTATCAGATCCCCGCTACACATCTGACTTACACTGATGGAAAACGTGTCTTTGAGTACCTGAATTCTACCAAGAACCCTTTTGGTCACGTTACTCCTGTCAAGACGGTAGAAGGAACAAAGCCAGCTCCATTTATGGCTTCATTCTCATCTCGTGGACCGAGTACCATTGAGCCATCAATCCTTAAGCCTGATATCACAGCACCAGGAGTGGATATAATTGCTGCTTATACTGGAGCACCTCTCCCACCTGATTTGGACATTGGCAAGCCGAGAAGACGCGACAACAAACGGGAGTCAATTCTTGATTCAACCAAGGTCTCAGCTACACCACTTGACTATGGTGCAGGCCATGTTGATCCAAATCGCGCAGCAAACCCCGGACTTGTCTATGACGTGACTACCGATGATTACTTGAATTTCTTATGTGCTCGTGGATACAATGCATCACTTCTCAAAATATTTTCCGACAAGCCACACAAATGTTCCAACACCTCTAGCATTATTGCTGATTTAAACTATCCTACAATTGCTGTTCCTGATCTCCAAGACAAACCAATTACCATTTCTAGAAAAGTGAAGAATGTCGGTACTCCAGGCACATATGTAGCATACGTTCGTGCACCAGCTAGAGTTTCTGTTAAGGTTCAGCCTAGCAGGTTGAAATTCAAGTCCATTGATGAAGAGAAGGAATTCAAGGTTACGCTGAAGGCAGATGGTAAGGTTCGAAATCCAGACTATGTGTTTGGAGAGTTGAAATGGTCGGATGGCAAGCACACTGTGAGGAGTCCTATTGCTGTGATGCGTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

717

Amino Acids

77.05

Weight (kDa)

8.67

Isoelectric Point (pI)

28.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 11 - 96 5.2e-17 Peptidase inhibitor I9
MORF_dom PF21864 39 - 95 1.6e-06 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 125 - 519 3e-22 Subtilase family
PA PF02225 388 - 463 3.8e-14 PA domain
fn3_6 PF17766 618 - 714 7.1e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 1989
AccB1I GGYRCC 1 cut(s) 985
AccB7I CCANNNNNTGG 1 cut(s) 761
AccII CGCG 4 cut(s) 729, 1236, 1623, 1710
AciI CCGC 6 cut(s) 95, 804, 911, 1234, 1236, 1356
AcoI YGGCCR 4 cut(s) 12, 413, 752, 801
AcsI RAATTY 5 cut(s) 103, 1405, 1759, 2006, 2033
AcuI CTGAAG 2 cut(s) 1182, 2069
AcvI CACGTG 1 cut(s) 611
AdeI CACNNNGTG 2 cut(s) 611, 2122
AfaI GTAC 8 cut(s) 44, 113, 336, 536, 784, 1400, 1507, 1933
AfiI CCNNNNNNNGG 3 cut(s) 344, 761, 965
AflII CTTAAG 1 cut(s) 1526
AflIII ACRYGT 1 cut(s) 1387
AgsI TTSAA 9 cut(s) 70, 645, 1183, 1653, 1759, 2005, 2011, 2038, 2098
AhlI ACTAGT 1 cut(s) 1034
AjiI CACGTC 1 cut(s) 1738
AjnI CCWGG 5 cut(s) 563, 615, 989, 1546, 1936
AjuI GAANNNNNNNTTGG 2 cut(s) 1891, 1923
AloI GAACNNNNNNTCC 2 cut(s) 1180, 1212
Alw21I GWGCWC 3 cut(s) 1582, 1774, 1966
Alw26I GTCTC 1 cut(s) 1666
Alw44I GTGCAC 1 cut(s) 1962
AoxI GGCC 8 cut(s) 12, 150, 413, 622, 752, 801, 956, 1690
ApaI GGGCCC 1 cut(s) 154
ApaLI GTGCAC 1 cut(s) 1962
ApeKI GCWGC 8 cut(s) 20, 200, 734, 815, 1145, 1271, 1565, 1712
ApoI RAATTY 5 cut(s) 103, 1405, 1759, 2006, 2033
ArsI GACNNNNNNTTYG 2 cut(s) 2069, 2101
Asp700I GAANNNNTTC 2 cut(s) 55, 2033
AspLEI GCGC 2 cut(s) 1286, 1712
AspS9I GGNCC 5 cut(s) 150, 151, 449, 956, 1499
AsuC2I CCSGG 2 cut(s) 669, 1722
AsuHPI GGTGA 4 cut(s) 386, 414, 597, 872
AsuII TTCGAA 1 cut(s) 2068
AsuNHI GCTAGC 1 cut(s) 1010
AvaII GGWCC 2 cut(s) 449, 1499
BaeGI GKGCMC 2 cut(s) 154, 1966
BalI TGGCCA 2 cut(s) 415, 754
BamHI GGATCC 2 cut(s) 241, 713
BanI GGYRCC 1 cut(s) 985
BanII GRGCYC 1 cut(s) 154
BauI CACGAG 2 cut(s) 1494, 1773
BbrPI CACGTG 1 cut(s) 611
BbsI GAAGAC 2 cut(s) 327, 1624
Bbv12I GWGCWC 3 cut(s) 1582, 1774, 1966
BbvI GCAGC 8 cut(s) 32, 187, 721, 802, 1157, 1258, 1552, 1724
BccI CCATC 7 cut(s) 466, 878, 1108, 1373, 1525, 2051, 2102
BcgI CGANNNNNNTGC 2 cut(s) 1134, 1168
BciT130I CCWGG 5 cut(s) 565, 617, 991, 1548, 1938
BciVI GTATCC 1 cut(s) 1772
BcnI CCSGG 2 cut(s) 669, 1722
BcoDI GTCTC 1 cut(s) 1666
BcuI ACTAGT 1 cut(s) 1034
BfaI CTAG 9 cut(s) 180, 317, 1011, 1035, 1839, 1911, 1971, 1995, 2152
BfmI CTRYAG 1 cut(s) 201
BfoI RGCGCY 1 cut(s) 1287
BfrI CTTAAG 1 cut(s) 1526
BfuAI ACCTGC 1 cut(s) 1989
BfuI GTATCC 1 cut(s) 1772
BlpI GCTNAGC 2 cut(s) 915, 940
Bme1390I CCNGG 7 cut(s) 565, 617, 669, 991, 1548, 1722, 1938
Bme18I GGWCC 2 cut(s) 449, 1499
BmgBI CACGTC 1 cut(s) 1738
BmgT120I GGNCC 5 cut(s) 150, 151, 449, 956, 1499
BmiI GGNNCC 6 cut(s) 152, 243, 715, 987, 1132, 1189
BmrFI CCNGG 7 cut(s) 565, 617, 669, 991, 1548, 1722, 1938
BmsI GCATC 4 cut(s) 802, 1072, 1796, 2133
BmtI GCTAGC 1 cut(s) 1014
BpiI GAAGAC 2 cut(s) 327, 1624
BplI GAGNNNNNCTC 2 cut(s) 1569, 1601
BpmI CTGGAG 2 cut(s) 1596, 1920
Bpu1102I GCTNAGC 2 cut(s) 915, 940
Bpu14I TTCGAA 1 cut(s) 2068
BpuMI CCSGG 2 cut(s) 669, 1722
Bsa29I ATCGAT 1 cut(s) 350
BsaAI YACGTR 1 cut(s) 611
BsaBI GATNNNNATC 2 cut(s) 1344, 1704
BsaI GGTCTC 1 cut(s) 1666
BsaJI CCNNGG 8 cut(s) 61, 625, 668, 964, 989, 1234, 1656, 1720
BsaXI ACNNNNNCTCC 2 cut(s) 1141, 1171
Bsc4I CCNNNNNNNGG 3 cut(s) 344, 761, 965
Bse118I RCCGGY 2 cut(s) 10, 1280
Bse1I ACTGG 2 cut(s) 409, 1579
Bse3DI GCAATG 2 cut(s) 148, 700
Bse8I GATNNNNATC 2 cut(s) 1344, 1704
BseBI CCWGG 5 cut(s) 565, 617, 991, 1548, 1938
BseCI ATCGAT 1 cut(s) 350
BseDI CCNNGG 8 cut(s) 61, 625, 668, 964, 989, 1234, 1656, 1720
BseGI GGATG 3 cut(s) 458, 889, 2113
BseJI GATNNNNATC 2 cut(s) 1344, 1704
BseLI CCNNNNNNNGG 3 cut(s) 344, 761, 965
BseMI GCAATG 2 cut(s) 148, 700
BseMII CTCAG 3 cut(s) 122, 954, 1677
BseNI ACTGG 2 cut(s) 409, 1579
BseRI GAGGAG 1 cut(s) 2140
BseSI GKGCMC 2 cut(s) 154, 1966
BseXI GCAGC 8 cut(s) 32, 187, 721, 802, 1157, 1258, 1552, 1724
BsgI GTGCAG 1 cut(s) 1707
Bsh1236I CGCG 4 cut(s) 729, 1236, 1623, 1710
BshFI GGCC 8 cut(s) 14, 152, 415, 624, 754, 803, 958, 1692
BshNI GGYRCC 1 cut(s) 985
BshVI ATCGAT 1 cut(s) 350
BsiHKAI GWGCWC 3 cut(s) 1582, 1774, 1966
BsiSI CCGG 4 cut(s) 11, 668, 1281, 1722
BslFI GGGAC 2 cut(s) 327, 982
BslI CCNNNNNNNGG 3 cut(s) 344, 761, 965
BsmAI GTCTC 1 cut(s) 1666
BsmFI GGGAC 2 cut(s) 327, 982
BsnI GGCC 8 cut(s) 14, 152, 415, 624, 754, 803, 958, 1692
Bso31I GGTCTC 1 cut(s) 1666
Bsp119I TTCGAA 1 cut(s) 2068
Bsp120I GGGCCC 1 cut(s) 150
Bsp1286I GDGCHC 4 cut(s) 154, 1582, 1774, 1966
Bsp1720I GCTNAGC 2 cut(s) 915, 940
Bsp19I CCATGG 2 cut(s) 61, 625
BspACI CCGC 6 cut(s) 95, 804, 911, 1234, 1236, 1356
BspANI GGCC 8 cut(s) 14, 152, 415, 624, 754, 803, 958, 1692
BspCNI CTCAG 3 cut(s) 121, 953, 1676
BspDI ATCGAT 1 cut(s) 350
BspFNI CGCG 4 cut(s) 729, 1236, 1623, 1710
BspLI GGNNCC 6 cut(s) 152, 243, 715, 987, 1132, 1189
BspMAI CTGCAG 1 cut(s) 205
BspMI ACCTGC 1 cut(s) 1989
BspOI GCTAGC 1 cut(s) 1014
BspT104I TTCGAA 1 cut(s) 2068
BspT107I GGYRCC 1 cut(s) 985
BspTI CTTAAG 1 cut(s) 1526
BspTNI GGTCTC 1 cut(s) 1666
BsrDI GCAATG 2 cut(s) 148, 700
BsrFI RCCGGY 2 cut(s) 10, 1280
BsrI ACTGG 2 cut(s) 409, 1579
BssAI RCCGGY 2 cut(s) 10, 1280
BssECI CCNNGG 8 cut(s) 61, 625, 668, 964, 989, 1234, 1656, 1720
BssSI CACGAG 2 cut(s) 1494, 1773
BssT1I CCWWGG 4 cut(s) 61, 625, 964, 1656
Bst2BI CACGAG 2 cut(s) 1494, 1773
Bst2UI CCWGG 5 cut(s) 565, 617, 991, 1548, 1938
Bst4CI ACNGT 7 cut(s) 30, 130, 830, 973, 1003, 1450, 2122
Bst6I CTCTTC 2 cut(s) 213, 2019
BstAFI CTTAAG 1 cut(s) 1526
BstBAI YACGTR 1 cut(s) 611
BstBI TTCGAA 1 cut(s) 2068
BstDEI CTNAG 6 cut(s) 108, 366, 915, 940, 1104, 1663
BstDSI CCRYGG 3 cut(s) 61, 625, 1234
BstENI CCTNNNNNAGG 1 cut(s) 963
BstF5I GGATG 3 cut(s) 458, 889, 2113
BstFNI CGCG 4 cut(s) 729, 1236, 1623, 1710
BstH2I RGCGCY 1 cut(s) 1287
BstHHI GCGC 2 cut(s) 1286, 1712
BstMAI GTCTC 1 cut(s) 1666
BstMWI GCNNNNNNNGC 8 cut(s) 20, 158, 731, 812, 945, 1145, 1268, 1277
BstNI CCWGG 5 cut(s) 565, 617, 991, 1548, 1938
BstSCI CCNGG 7 cut(s) 563, 615, 667, 989, 1546, 1720, 1936
BstSFI CTRYAG 1 cut(s) 201
BstSLI GKGCMC 2 cut(s) 154, 1966
BstUI CGCG 4 cut(s) 729, 1236, 1623, 1710
BstV1I GCAGC 8 cut(s) 32, 187, 721, 802, 1157, 1258, 1552, 1724
BstV2I GAAGAC 2 cut(s) 327, 1624
BstX2I RGATCY 3 cut(s) 241, 713, 1350
BstXI CCANNNNNNTGG 1 cut(s) 465
BstYI RGATCY 3 cut(s) 241, 713, 1350
Bsu15I ATCGAT 1 cut(s) 350
BsuI GTATCC 1 cut(s) 1772
BsuRI GGCC 8 cut(s) 14, 152, 415, 624, 754, 803, 958, 1692
BsuTUI ATCGAT 1 cut(s) 350
BtgI CCRYGG 3 cut(s) 61, 625, 1234
BtgZI GCGATG 1 cut(s) 450
BtrI CACGTC 1 cut(s) 1738
BtsCI GGATG 3 cut(s) 458, 889, 2113
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 6 cut(s) 402, 699, 829, 978, 1374, 2118
BveI ACCTGC 1 cut(s) 1989
CfoI GCGC 2 cut(s) 1286, 1712
Cfr10I RCCGGY 2 cut(s) 10, 1280
Cfr13I GGNCC 5 cut(s) 150, 151, 449, 956, 1499
Cfr42I CCGCGG 1 cut(s) 1237
ClaI ATCGAT 1 cut(s) 350
CseI GACGC 2 cut(s) 1629, 2135
Csp6I GTAC 8 cut(s) 43, 112, 335, 535, 783, 1399, 1506, 1932
CspCI CAANNNNNGTGG 4 cut(s) 451, 486, 1578, 1613
CviAII CATG 9 cut(s) 62, 300, 310, 626, 689, 799, 908, 920, 1694
CviQI GTAC 8 cut(s) 43, 112, 335, 535, 783, 1399, 1506, 1932
DdeI CTNAG 6 cut(s) 108, 366, 915, 940, 1104, 1663
DraI TTTAAA 1 cut(s) 1857
DraIII CACNNNGTG 2 cut(s) 611, 2122
DrdI GACNNNNNNGTC 1 cut(s) 407
DseDI GACNNNNNNGTC 1 cut(s) 407
EaeI YGGCCR 4 cut(s) 12, 413, 752, 801
Eam1104I CTCTTC 2 cut(s) 213, 2019
EarI CTCTTC 2 cut(s) 213, 2019
Eco130I CCWWGG 4 cut(s) 61, 625, 964, 1656
Eco24I GRGCYC 1 cut(s) 154
Eco31I GGTCTC 1 cut(s) 1666
Eco32I GATATC 1 cut(s) 1537
Eco47I GGWCC 2 cut(s) 449, 1499
Eco57I CTGAAG 2 cut(s) 1182, 2069
Eco72I CACGTG 1 cut(s) 611
EcoNI CCTNNNNNAGG 1 cut(s) 963
EcoO109I RGGNCCY 2 cut(s) 150, 956
EcoRI GAATTC 3 cut(s) 103, 1405, 2033
EcoRII CCWGG 5 cut(s) 563, 615, 989, 1546, 1936
EcoRV GATATC 1 cut(s) 1537
EcoT14I CCWWGG 4 cut(s) 61, 625, 964, 1656
EcoT22I ATGCAT 1 cut(s) 1789
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 4 cut(s) 61, 625, 964, 1656
FaeI CATG 9 cut(s) 65, 303, 313, 629, 692, 802, 911, 923, 1697
FalI AAGNNNNNCTT 2 cut(s) 1069, 1101
FaqI GGGAC 2 cut(s) 327, 982
FatI CATG 9 cut(s) 61, 299, 309, 625, 688, 798, 907, 919, 1693
FauI CCCGC 2 cut(s) 1229, 1363
FauNDI CATATG 1 cut(s) 1945
FokI GGATG 3 cut(s) 445, 896, 2120
FriOI GRGCYC 1 cut(s) 154
FspBI CTAG 9 cut(s) 180, 317, 1011, 1035, 1839, 1911, 1971, 1995, 2152
GlaI GCGC 2 cut(s) 1285, 1711
GsuI CTGGAG 2 cut(s) 1596, 1920
HaeII RGCGCY 1 cut(s) 1287
HaeIII GGCC 8 cut(s) 14, 152, 415, 624, 754, 803, 958, 1692
HapII CCGG 4 cut(s) 11, 668, 1281, 1722
HgaI GACGC 2 cut(s) 1629, 2135
HhaI GCGC 2 cut(s) 1286, 1712
Hin1II CATG 9 cut(s) 65, 303, 313, 629, 692, 802, 911, 923, 1697
Hin6I GCGC 2 cut(s) 1284, 1710
HinP1I GCGC 2 cut(s) 1284, 1710
HincII GTYRAC 1 cut(s) 1306
HindII GTYRAC 1 cut(s) 1306
HindIII AAGCTT 1 cut(s) 757
HinfI GANTC 9 cut(s) 419, 557, 595, 863, 924, 1166, 1637, 1649, 2128
HpaI GTTAAC 1 cut(s) 1306
HpaII CCGG 4 cut(s) 11, 668, 1281, 1722
HphI GGTGA 4 cut(s) 386, 414, 597, 872
Hpy166II GTNNAC 6 cut(s) 43, 783, 1032, 1306, 1499, 1964
Hpy188I TCNGA 5 cut(s) 527, 1350, 1368, 1810, 2107
Hpy188III TCNNGA 8 cut(s) 1025, 1089, 1160, 1444, 1646, 1880, 1911, 2075
Hpy8I GTNNAC 6 cut(s) 43, 783, 1032, 1306, 1499, 1964
Hpy99I CGWCG 1 cut(s) 2151
HpyAV CCTTC 7 cut(s) 434, 614, 767, 1198, 1448, 2023, 2044
HpyCH4III ACNGT 7 cut(s) 30, 130, 830, 973, 1003, 1450, 2122
HpyCH4IV ACGT 6 cut(s) 77, 610, 1387, 1431, 1737, 1956
HpyF10VI GCNNNNNNNGC 8 cut(s) 20, 158, 731, 812, 945, 1145, 1268, 1277
HpyF3I CTNAG 6 cut(s) 108, 366, 915, 940, 1104, 1663
HpySE526I ACGT 6 cut(s) 77, 610, 1387, 1431, 1737, 1956
Hsp92II CATG 9 cut(s) 65, 303, 313, 629, 692, 802, 911, 923, 1697
HspAI GCGC 2 cut(s) 1284, 1710
KroI GCCGGC 2 cut(s) 10, 1280
KroNI GCCGGC 2 cut(s) 12, 1282
KspAI GTTAAC 1 cut(s) 1306
KspI CCGCGG 1 cut(s) 1237
LmnI GCTCC 5 cut(s) 527, 1007, 1130, 1474, 1577
Lsp1109I GCAGC 8 cut(s) 32, 187, 721, 802, 1157, 1258, 1552, 1724
LweI GCATC 4 cut(s) 802, 1072, 1796, 2133
MaeI CTAG 9 cut(s) 180, 317, 1011, 1035, 1839, 1911, 1971, 1995, 2152
MaeII ACGT 6 cut(s) 77, 610, 1387, 1431, 1737, 1956
MaeIII GTNAC 9 cut(s) 341, 603, 611, 824, 973, 1427, 1432, 1738, 2041
MfeI CAATTG 1 cut(s) 1869
MflI RGATCY 3 cut(s) 241, 713, 1350
MhlI GDGCHC 4 cut(s) 154, 1582, 1774, 1966
MlsI TGGCCA 2 cut(s) 415, 754
MluNI TGGCCA 2 cut(s) 415, 754
MlyI GAGTC 3 cut(s) 566, 1646, 2137
MmeI TCCRAC 5 cut(s) 985, 1270, 1833, 1854, 2085
Mox20I TGGCCA 2 cut(s) 415, 754
Mph1103I ATGCAT 1 cut(s) 1789
MroNI GCCGGC 2 cut(s) 10, 1280
MroXI GAANNNNTTC 2 cut(s) 55, 2033
MscI TGGCCA 2 cut(s) 415, 754
MseI TTAA 8 cut(s) 278, 540, 998, 1209, 1305, 1527, 1856, 1983
MslI CAYNNNNRTG 4 cut(s) 60, 463, 693, 1822
Msp20I TGGCCA 2 cut(s) 415, 754
MspA1I CMGCKG 3 cut(s) 650, 944, 1236
MspCI CTTAAG 1 cut(s) 1526
MspI CCGG 4 cut(s) 11, 668, 1281, 1722
MspR9I CCNGG 7 cut(s) 565, 617, 669, 991, 1548, 1722, 1938
MunI CAATTG 1 cut(s) 1869
MvaI CCWGG 5 cut(s) 565, 617, 991, 1548, 1938
MvnI CGCG 4 cut(s) 729, 1236, 1623, 1710
MwoI GCNNNNNNNGC 8 cut(s) 20, 158, 731, 812, 945, 1145, 1268, 1277
NaeI GCCGGC 2 cut(s) 12, 1282
NciI CCSGG 2 cut(s) 669, 1722
NcoI CCATGG 2 cut(s) 61, 625
NdeI CATATG 1 cut(s) 1945
NgoMIV GCCGGC 2 cut(s) 10, 1280
NheI GCTAGC 1 cut(s) 1010
NlaIII CATG 9 cut(s) 65, 303, 313, 629, 692, 802, 911, 923, 1697
NlaIV GGNNCC 6 cut(s) 152, 243, 715, 987, 1132, 1189
NmeAIII GCCGAG 1 cut(s) 1638
NmuCI GTSAC 7 cut(s) 341, 603, 611, 824, 973, 1427, 1738
NsiI ATGCAT 1 cut(s) 1789
NspV TTCGAA 1 cut(s) 2068
PcsI WCGNNNNNNNCGW 1 cut(s) 1743
PdiI GCCGGC 2 cut(s) 12, 1282
PdmI GAANNNNTTC 2 cut(s) 55, 2033
PfeI GAWTC 6 cut(s) 419, 595, 863, 924, 1166, 1649
PflMI CCANNNNNTGG 1 cut(s) 761
PleI GAGTC 3 cut(s) 565, 1645, 2136
PmaCI CACGTG 1 cut(s) 611
PmlI CACGTG 1 cut(s) 611
PpsI GAGTC 3 cut(s) 565, 1645, 2136
Ppu21I YACGTR 1 cut(s) 611
Psp6I CCWGG 5 cut(s) 563, 615, 989, 1546, 1936
PspCI CACGTG 1 cut(s) 611
PspGI CCWGG 5 cut(s) 563, 615, 989, 1546, 1936
PspN4I GGNNCC 6 cut(s) 152, 243, 715, 987, 1132, 1189
PspOMI GGGCCC 1 cut(s) 150
PspPI GGNCC 5 cut(s) 150, 151, 449, 956, 1499
PsrI GAACNNNNNNTAC 2 cut(s) 1941, 1973
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 3 cut(s) 241, 713, 1350
PvuII CAGCTG 2 cut(s) 650, 944
RsaI GTAC 8 cut(s) 44, 113, 336, 536, 784, 1400, 1507, 1933
RsaNI GTAC 8 cut(s) 43, 112, 335, 535, 783, 1399, 1506, 1932
RseI CAYNNNNRTG 4 cut(s) 60, 463, 693, 1822
SacII CCGCGG 1 cut(s) 1237
SaqAI TTAA 8 cut(s) 278, 540, 998, 1209, 1305, 1527, 1856, 1983
Sau96I GGNCC 5 cut(s) 150, 151, 449, 956, 1499
SchI GAGTC 3 cut(s) 566, 1646, 2137
ScrFI CCNGG 7 cut(s) 565, 617, 669, 991, 1548, 1722, 1938
SduI GDGCHC 4 cut(s) 154, 1582, 1774, 1966
SfaNI GCATC 4 cut(s) 802, 1072, 1796, 2133
SfcI CTRYAG 1 cut(s) 201
Sfr303I CCGCGG 1 cut(s) 1237
SfuI TTCGAA 1 cut(s) 2068
SgrBI CCGCGG 1 cut(s) 1237
SinI GGWCC 2 cut(s) 449, 1499
SmiMI CAYNNNNRTG 4 cut(s) 60, 463, 693, 1822
SmlI CTYRAG 1 cut(s) 1526
SmoI CTYRAG 1 cut(s) 1526
SpeI ACTAGT 1 cut(s) 1034
SsiI CCGC 6 cut(s) 95, 804, 911, 1234, 1236, 1356
SspI AATATT 1 cut(s) 1803
SspMI CTAG 9 cut(s) 180, 317, 1011, 1035, 1839, 1911, 1971, 1995, 2152
StyD4I CCNGG 7 cut(s) 563, 615, 667, 989, 1546, 1720, 1936
StyI CCWWGG 4 cut(s) 61, 625, 964, 1656
TaaI ACNGT 7 cut(s) 30, 130, 830, 973, 1003, 1450, 2122
TaiI ACGT 6 cut(s) 80, 613, 1390, 1434, 1740, 1959
TaqI TCGA 4 cut(s) 84, 90, 350, 2068
TaqII GACCGA 1 cut(s) 1516
TatI WGTACW 3 cut(s) 42, 111, 782
TauI GCSGC 3 cut(s) 98, 806, 914
TfiI GAWTC 6 cut(s) 419, 595, 863, 924, 1166, 1649
Tru1I TTAA 8 cut(s) 278, 540, 998, 1209, 1305, 1527, 1856, 1983
Tru9I TTAA 8 cut(s) 278, 540, 998, 1209, 1305, 1527, 1856, 1983
TscAI CASTG 6 cut(s) 409, 706, 829, 978, 1381, 2125
TseFI GTSAC 7 cut(s) 341, 603, 611, 824, 973, 1427, 1738
TseI GCWGC 8 cut(s) 20, 200, 734, 815, 1145, 1271, 1565, 1712
Tsp45I GTSAC 7 cut(s) 341, 603, 611, 824, 973, 1427, 1738
TspDTI ATGAA 5 cut(s) 288, 298, 453, 1473, 2037
TspGWI ACGGA 1 cut(s) 360
TspRI CASTG 6 cut(s) 409, 706, 829, 978, 1381, 2125
Van91I CCANNNNNTGG 1 cut(s) 761
Vha464I CTTAAG 1 cut(s) 1526
VneI GTGCAC 1 cut(s) 1962
VpaK11BI GGWCC 2 cut(s) 449, 1499
XagI CCTNNNNNAGG 1 cut(s) 963
XapI RAATTY 5 cut(s) 103, 1405, 1759, 2006, 2033
XbaI TCTAGA 1 cut(s) 1910
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XmnI GAANNNNTTC 2 cut(s) 55, 2033
XspI CTAG 9 cut(s) 180, 317, 1011, 1035, 1839, 1911, 1971, 1995, 2152
Zsp2I ATGCAT 1 cut(s) 1789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.