subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2454 |
| AarI | CACCTGC | 2 cut(s) | 1232, 1859 |
| AbsI | CCTCGAGG | 1 cut(s) | 2793 |
| Acc36I | ACCTGC | 3 cut(s) | 474, 1232, 1859 |
| AccB1I | GGYRCC | 1 cut(s) | 2683 |
| AccII | CGCG | 2 cut(s) | 108, 195 |
| AccIII | TCCGGA | 1 cut(s) | 2255 |
| AciI | CCGC | 2 cut(s) | 66, 2060 |
| AclWI | GGATC | 7 cut(s) | 179, 836, 849, 1225, 1772, 1922, 2659 |
| AcoI | YGGCCR | 2 cut(s) | 1019, 1340 |
| AcsI | RAATTY | 8 cut(s) | 244, 491, 518, 703, 904, 1755, 2479, 2726 |
| AcuI | CTGAAG | 3 cut(s) | 554, 924, 2184 |
| AfaI | GTAC | 3 cut(s) | 418, 644, 2666 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 871, 900, 1234, 1352, 1547, 2603 |
| AflIII | ACRYGT | 1 cut(s) | 1975 |
| AjnI | CCWGG | 5 cut(s) | 421, 1255, 1546, 2134, 2656 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 376, 408, 2522, 2554 |
| Alw21I | GWGCWC | 5 cut(s) | 259, 655, 1774, 1941, 2494 |
| Alw26I | GTCTC | 3 cut(s) | 151, 1437, 2562 |
| AlwI | GGATC | 7 cut(s) | 179, 836, 849, 1225, 1772, 1922, 2659 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1874 |
| Ama87I | CYCGRG | 3 cut(s) | 2440, 2493, 2793 |
| Aor13HI | TCCGGA | 1 cut(s) | 2255 |
| AoxI | GGCC | 7 cut(s) | 175, 1019, 1168, 1340, 1857, 2087, 2174 |
| ApoI | RAATTY | 8 cut(s) | 244, 491, 518, 703, 904, 1755, 2479, 2726 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 554, 586, 2661, 2693 |
| AseI | ATTAAT | 1 cut(s) | 789 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 518, 2753 |
| AspA2I | CCTAGG | 1 cut(s) | 1781 |
| AspLEI | GCGC | 2 cut(s) | 197, 2432 |
| AspS9I | GGNCC | 8 cut(s) | 665, 1055, 1169, 1482, 1538, 2087, 2174, 2437 |
| AsuC2I | CCSGG | 5 cut(s) | 207, 895, 2204, 2441, 2442 |
| AsuHPI | GGTGA | 5 cut(s) | 518, 2152, 2646, 2753, 2828 |
| AsuII | TTCGAA | 1 cut(s) | 553 |
| AsuNHI | GCTAGC | 1 cut(s) | 1592 |
| AvaI | CYCGRG | 3 cut(s) | 2440, 2493, 2793 |
| AvaII | GGWCC | 5 cut(s) | 665, 1055, 1482, 1538, 2437 |
| AvrII | CCTAGG | 1 cut(s) | 1781 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 990, 1023 |
| BalI | TGGCCA | 2 cut(s) | 1021, 1342 |
| BamHI | GGATCC | 1 cut(s) | 841 |
| BanI | GGYRCC | 1 cut(s) | 2683 |
| BanII | GRGCYC | 3 cut(s) | 65, 1774, 2300 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2623, 2655 |
| BauI | CACGAG | 1 cut(s) | 258 |
| BbsI | GAAGAC | 3 cut(s) | 26, 109, 853 |
| Bbv12I | GWGCWC | 5 cut(s) | 259, 655, 1774, 1941, 2494 |
| BccI | CCATC | 6 cut(s) | 536, 587, 682, 1202, 2292, 2822 |
| BceAI | ACGGC | 2 cut(s) | 1251, 1984 |
| BciT130I | CCWGG | 5 cut(s) | 423, 1257, 1548, 2136, 2658 |
| BciVI | GTATCC | 3 cut(s) | 257, 2263, 2492 |
| BclI | TGATCA | 2 cut(s) | 1704, 2514 |
| BcnI | CCSGG | 5 cut(s) | 207, 895, 2204, 2441, 2442 |
| BcoDI | GTCTC | 3 cut(s) | 151, 1437, 2562 |
| BfmI | CTRYAG | 3 cut(s) | 1239, 1713, 1875 |
| BfuAI | ACCTGC | 3 cut(s) | 474, 1232, 1859 |
| BfuI | GTATCC | 3 cut(s) | 257, 2263, 2492 |
| BlnI | CCTAGG | 1 cut(s) | 1781 |
| Bme18I | GGWCC | 5 cut(s) | 665, 1055, 1482, 1538, 2437 |
| BmeT110I | CYCGRG | 3 cut(s) | 2440, 2493, 2793 |
| BmgT120I | GGNCC | 8 cut(s) | 665, 1055, 1169, 1482, 1538, 2087, 2174, 2437 |
| BmiI | GGNNCC | 9 cut(s) | 62, 843, 1056, 1288, 1483, 2088, 2297, 2439, 2685 |
| BmsI | GCATC | 7 cut(s) | 281, 1384, 1708, 1717, 1723, 1745, 2352 |
| BmtI | GCTAGC | 1 cut(s) | 1596 |
| BpiI | GAAGAC | 3 cut(s) | 26, 109, 853 |
| BpmI | CTGGAG | 3 cut(s) | 405, 2010, 2120 |
| Bpu10I | CCTNAGC | 2 cut(s) | 837, 2775 |
| Bpu14I | TTCGAA | 1 cut(s) | 553 |
| BpuEI | CTTGAG | 2 cut(s) | 754, 1293 |
| BpuMI | CCSGG | 5 cut(s) | 207, 895, 2204, 2441, 2442 |
| BsaAI | YACGTR | 1 cut(s) | 2664 |
| BsaBI | GATNNNNATC | 2 cut(s) | 189, 2832 |
| BsaI | GGTCTC | 1 cut(s) | 151 |
| BsaWI | WCCGGW | 1 cut(s) | 2255 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1251, 1281, 1990, 2020 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 871, 900, 1234, 1352, 1547, 2603 |
| Bse118I | RCCGGY | 2 cut(s) | 1166, 2225 |
| Bse1I | ACTGG | 3 cut(s) | 1006, 1747, 2027 |
| Bse3DI | GCAATG | 7 cut(s) | 1108, 1282, 1288, 1768, 2127, 2359, 2439 |
| Bse8I | GATNNNNATC | 2 cut(s) | 189, 2832 |
| BseAI | TCCGGA | 1 cut(s) | 2255 |
| BseBI | CCWGG | 5 cut(s) | 423, 1257, 1548, 2136, 2658 |
| BseGI | GGATG | 3 cut(s) | 598, 1736, 2284 |
| BseJI | GATNNNNATC | 2 cut(s) | 189, 2832 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 871, 900, 1234, 1352, 1547, 2603 |
| BseMI | GCAATG | 7 cut(s) | 1108, 1282, 1288, 1768, 2127, 2359, 2439 |
| BseMII | CTCAG | 3 cut(s) | 162, 842, 2264 |
| BseNI | ACTGG | 3 cut(s) | 1006, 1747, 2027 |
| BseRI | GAGGAG | 2 cut(s) | 1296, 1466 |
| BseYI | CCCAGC | 3 cut(s) | 1051, 2299, 2581 |
| BsgI | GTGCAG | 4 cut(s) | 192, 1542, 2427, 2628 |
| Bsh1236I | CGCG | 2 cut(s) | 108, 195 |
| BshFI | GGCC | 7 cut(s) | 177, 1021, 1170, 1342, 1859, 2089, 2176 |
| BshNI | GGYRCC | 1 cut(s) | 2683 |
| BsiHKAI | GWGCWC | 5 cut(s) | 259, 655, 1774, 1941, 2494 |
| BsiHKCI | CYCGRG | 3 cut(s) | 2440, 2493, 2793 |
| BsiSI | CCGG | 7 cut(s) | 207, 895, 1167, 2203, 2226, 2256, 2441 |
| BslFI | GGGAC | 4 cut(s) | 1046, 1068, 1564, 2532 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 871, 900, 1234, 1352, 1547, 2603 |
| BsmAI | GTCTC | 3 cut(s) | 151, 1437, 2562 |
| BsmFI | GGGAC | 4 cut(s) | 1046, 1068, 1564, 2532 |
| BsnI | GGCC | 7 cut(s) | 177, 1021, 1170, 1342, 1859, 2089, 2176 |
| Bso31I | GGTCTC | 1 cut(s) | 151 |
| BsoBI | CYCGRG | 3 cut(s) | 2440, 2493, 2793 |
| Bsp119I | TTCGAA | 1 cut(s) | 553 |
| Bsp1286I | GDGCHC | 7 cut(s) | 65, 259, 655, 1774, 1941, 2300, 2494 |
| Bsp13I | TCCGGA | 1 cut(s) | 2255 |
| Bsp19I | CCATGG | 1 cut(s) | 1571 |
| BspACI | CCGC | 2 cut(s) | 66, 2060 |
| BspANI | GGCC | 7 cut(s) | 177, 1021, 1170, 1342, 1859, 2089, 2176 |
| BspCNI | CTCAG | 3 cut(s) | 161, 841, 2265 |
| BspEI | TCCGGA | 1 cut(s) | 2255 |
| BspFNI | CGCG | 2 cut(s) | 108, 195 |
| BspHI | TCATGA | 1 cut(s) | 2322 |
| BspLI | GGNNCC | 9 cut(s) | 62, 843, 1056, 1288, 1483, 2088, 2297, 2439, 2685 |
| BspMAI | CTGCAG | 2 cut(s) | 1243, 1717 |
| BspMI | ACCTGC | 3 cut(s) | 474, 1232, 1859 |
| BspOI | GCTAGC | 1 cut(s) | 1596 |
| BspPI | GGATC | 7 cut(s) | 179, 836, 849, 1225, 1772, 1922, 2659 |
| BspQI | GCTCTTC | 1 cut(s) | 1841 |
| BspT104I | TTCGAA | 1 cut(s) | 553 |
| BspT107I | GGYRCC | 1 cut(s) | 2683 |
| BspTNI | GGTCTC | 1 cut(s) | 151 |
| BsrDI | GCAATG | 7 cut(s) | 1108, 1282, 1288, 1768, 2127, 2359, 2439 |
| BsrFI | RCCGGY | 2 cut(s) | 1166, 2225 |
| BsrI | ACTGG | 3 cut(s) | 1006, 1747, 2027 |
| BssAI | RCCGGY | 2 cut(s) | 1166, 2225 |
| BssSI | CACGAG | 1 cut(s) | 258 |
| BssT1I | CCWWGG | 4 cut(s) | 141, 1293, 1571, 1781 |
| Bst2BI | CACGAG | 1 cut(s) | 258 |
| Bst2UI | CCWGG | 5 cut(s) | 423, 1257, 1548, 2136, 2658 |
| Bst4CI | ACNGT | 5 cut(s) | 1195, 1412, 1555, 1585, 2623 |
| Bst6I | CTCTTC | 3 cut(s) | 504, 807, 1841 |
| BstBAI | YACGTR | 1 cut(s) | 2664 |
| BstBI | TTCGAA | 1 cut(s) | 553 |
| BstDEI | CTNAG | 7 cut(s) | 148, 646, 828, 837, 1381, 2273, 2775 |
| BstDSI | CCRYGG | 2 cut(s) | 1233, 1571 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 869 |
| BstF5I | GGATG | 3 cut(s) | 598, 1736, 2284 |
| BstFNI | CGCG | 2 cut(s) | 108, 195 |
| BstHHI | GCGC | 2 cut(s) | 197, 2432 |
| BstMAI | GTCTC | 3 cut(s) | 151, 1437, 2562 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 833, 1163, 1319, 1394, 1718, 1727, 1865, 2059, 2405 |
| BstNI | CCWGG | 5 cut(s) | 423, 1257, 1548, 2136, 2658 |
| BstNSI | RCATGY | 3 cut(s) | 1855, 1979, 2244 |
| BstSFI | CTRYAG | 3 cut(s) | 1239, 1713, 1875 |
| BstUI | CGCG | 2 cut(s) | 108, 195 |
| BstV2I | GAAGAC | 3 cut(s) | 26, 109, 853 |
| BstX2I | RGATCY | 1 cut(s) | 841 |
| BstYI | RGATCY | 1 cut(s) | 841 |
| BsuI | GTATCC | 3 cut(s) | 257, 2263, 2492 |
| BsuRI | GGCC | 7 cut(s) | 177, 1021, 1170, 1342, 1859, 2089, 2176 |
| BtgI | CCRYGG | 2 cut(s) | 1233, 1571 |
| BtsCI | GGATG | 3 cut(s) | 598, 1736, 2284 |
| BtsIMutI | CAGTG | 3 cut(s) | 1191, 1714, 1740 |
| BveI | ACCTGC | 3 cut(s) | 474, 1232, 1859 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1874 |
| CciI | TCATGA | 1 cut(s) | 2322 |
| CfoI | GCGC | 2 cut(s) | 197, 2432 |
| Cfr10I | RCCGGY | 2 cut(s) | 1166, 2225 |
| Cfr13I | GGNCC | 8 cut(s) | 665, 1055, 1169, 1482, 1538, 2087, 2174, 2437 |
| Cfr9I | CCCGGG | 1 cut(s) | 2440 |
| CseI | GACGC | 2 cut(s) | 114, 2665 |
| Csp6I | GTAC | 3 cut(s) | 417, 643, 2665 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1057, 1092 |
| CviQI | GTAC | 3 cut(s) | 417, 643, 2665 |
| DdeI | CTNAG | 7 cut(s) | 148, 646, 828, 837, 1381, 2273, 2775 |
| DraI | TTTAAA | 1 cut(s) | 342 |
| EaeI | YGGCCR | 2 cut(s) | 1019, 1340 |
| Eam1104I | CTCTTC | 3 cut(s) | 504, 807, 1841 |
| EarI | CTCTTC | 3 cut(s) | 504, 807, 1841 |
| Ecl136II | GAGCTC | 1 cut(s) | 1772 |
| Eco130I | CCWWGG | 4 cut(s) | 141, 1293, 1571, 1781 |
| Eco24I | GRGCYC | 3 cut(s) | 65, 1774, 2300 |
| Eco31I | GGTCTC | 1 cut(s) | 151 |
| Eco32I | GATATC | 2 cut(s) | 855, 2125 |
| Eco47I | GGWCC | 5 cut(s) | 665, 1055, 1482, 1538, 2437 |
| Eco53kI | GAGCTC | 1 cut(s) | 1772 |
| Eco57I | CTGAAG | 3 cut(s) | 554, 924, 2184 |
| Eco88I | CYCGRG | 3 cut(s) | 2440, 2493, 2793 |
| EcoICRI | GAGCTC | 1 cut(s) | 1772 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 869 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1055, 1482, 2087 |
| EcoRI | GAATTC | 1 cut(s) | 518 |
| EcoRII | CCWGG | 5 cut(s) | 421, 1255, 1546, 2134, 2656 |
| EcoRV | GATATC | 2 cut(s) | 855, 2125 |
| EcoT14I | CCWWGG | 4 cut(s) | 141, 1293, 1571, 1781 |
| EcoT22I | ATGCAT | 3 cut(s) | 274, 1738, 2806 |
| EcoT38I | GRGCYC | 3 cut(s) | 65, 1774, 2300 |
| ErhI | CCWWGG | 4 cut(s) | 141, 1293, 1571, 1781 |
| FaqI | GGGAC | 4 cut(s) | 1046, 1068, 1564, 2532 |
| FauI | CCCGC | 1 cut(s) | 73 |
| FauNDI | CATATG | 2 cut(s) | 430, 439 |
| FbaI | TGATCA | 2 cut(s) | 1704, 2514 |
| FokI | GGATG | 3 cut(s) | 605, 1723, 2271 |
| FriOI | GRGCYC | 3 cut(s) | 65, 1774, 2300 |
| GlaI | GCGC | 2 cut(s) | 196, 2431 |
| GsaI | CCCAGC | 3 cut(s) | 1055, 2303, 2585 |
| GsuI | CTGGAG | 3 cut(s) | 405, 2010, 2120 |
| HaeIII | GGCC | 7 cut(s) | 177, 1021, 1170, 1342, 1859, 2089, 2176 |
| HapII | CCGG | 7 cut(s) | 207, 895, 1167, 2203, 2226, 2256, 2441 |
| HgaI | GACGC | 2 cut(s) | 114, 2665 |
| HhaI | GCGC | 2 cut(s) | 197, 2432 |
| Hin6I | GCGC | 2 cut(s) | 195, 2430 |
| HinP1I | GCGC | 2 cut(s) | 195, 2430 |
| HincII | GTYRAC | 1 cut(s) | 1088 |
| HindII | GTYRAC | 1 cut(s) | 1088 |
| HindIII | AAGCTT | 2 cut(s) | 1033, 2719 |
| HinfI | GANTC | 7 cut(s) | 122, 134, 934, 944, 1025, 2369, 2848 |
| HpaII | CCGG | 7 cut(s) | 207, 895, 1167, 2203, 2226, 2256, 2441 |
| HphI | GGTGA | 5 cut(s) | 518, 2152, 2646, 2753, 2828 |
| Hpy166II | GTNNAC | 3 cut(s) | 643, 1088, 1614 |
| Hpy8I | GTNNAC | 3 cut(s) | 643, 1088, 1614 |
| Hpy99I | CGWCG | 2 cut(s) | 1186, 1970 |
| HpyCH4III | ACNGT | 5 cut(s) | 1195, 1412, 1555, 1585, 2623 |
| HpyCH4IV | ACGT | 1 cut(s) | 2663 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 833, 1163, 1319, 1394, 1718, 1727, 1865, 2059, 2405 |
| HpyF3I | CTNAG | 7 cut(s) | 148, 646, 828, 837, 1381, 2273, 2775 |
| HpySE526I | ACGT | 1 cut(s) | 2663 |
| HspAI | GCGC | 2 cut(s) | 195, 2430 |
| Kpn2I | TCCGGA | 1 cut(s) | 2255 |
| KroI | GCCGGC | 1 cut(s) | 1166 |
| KroNI | GCCGGC | 1 cut(s) | 1168 |
| Ksp22I | TGATCA | 2 cut(s) | 1704, 2514 |
| LguI | GCTCTTC | 1 cut(s) | 1841 |
| LmnI | GCTCC | 9 cut(s) | 60, 650, 1655, 1769, 1944, 2062, 2074, 2101, 2295 |
| LweI | GCATC | 7 cut(s) | 281, 1384, 1708, 1717, 1723, 1745, 2352 |
| MaeII | ACGT | 1 cut(s) | 2663 |
| MaeIII | GTNAC | 7 cut(s) | 526, 1199, 1406, 1579, 2617, 2623, 2638 |
| MfeI | CAATTG | 3 cut(s) | 354, 1646, 2586 |
| MflI | RGATCY | 1 cut(s) | 841 |
| MhlI | GDGCHC | 7 cut(s) | 65, 259, 655, 1774, 1941, 2300, 2494 |
| MlsI | TGGCCA | 2 cut(s) | 1021, 1342 |
| MluNI | TGGCCA | 2 cut(s) | 1021, 1342 |
| MlyI | GAGTC | 3 cut(s) | 131, 938, 2363 |
| MmeI | TCCRAC | 5 cut(s) | 318, 339, 570, 1747, 2629 |
| Mox20I | TGGCCA | 2 cut(s) | 1021, 1342 |
| Mph1103I | ATGCAT | 3 cut(s) | 274, 1738, 2806 |
| MroI | TCCGGA | 1 cut(s) | 2255 |
| MroNI | GCCGGC | 1 cut(s) | 1166 |
| MroXI | GAANNNNTTC | 2 cut(s) | 518, 2753 |
| MscI | TGGCCA | 2 cut(s) | 1021, 1342 |
| MslI | CAYNNNNRTG | 5 cut(s) | 307, 660, 789, 1511, 2234 |
| Msp20I | TGGCCA | 2 cut(s) | 1021, 1342 |
| MspA1I | CMGCKG | 2 cut(s) | 68, 1526 |
| MspI | CCGG | 7 cut(s) | 207, 895, 1167, 2203, 2226, 2256, 2441 |
| MunI | CAATTG | 3 cut(s) | 354, 1646, 2586 |
| MvaI | CCWGG | 5 cut(s) | 423, 1257, 1548, 2136, 2658 |
| MvnI | CGCG | 2 cut(s) | 108, 195 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 833, 1163, 1319, 1394, 1718, 1727, 1865, 2059, 2405 |
| NaeI | GCCGGC | 1 cut(s) | 1168 |
| NciI | CCSGG | 5 cut(s) | 207, 895, 2204, 2441, 2442 |
| NcoI | CCATGG | 1 cut(s) | 1571 |
| NdeI | CATATG | 2 cut(s) | 430, 439 |
| NgoMIV | GCCGGC | 1 cut(s) | 1166 |
| NheI | GCTAGC | 1 cut(s) | 1592 |
| NlaIV | GGNNCC | 9 cut(s) | 62, 843, 1056, 1288, 1483, 2088, 2297, 2439, 2685 |
| NmuCI | GTSAC | 3 cut(s) | 1199, 1406, 2617 |
| NsiI | ATGCAT | 3 cut(s) | 274, 1738, 2806 |
| NspI | RCATGY | 3 cut(s) | 1855, 1979, 2244 |
| NspV | TTCGAA | 1 cut(s) | 553 |
| PaeI | GCATGC | 2 cut(s) | 1855, 2244 |
| PaeR7I | CTCGAG | 2 cut(s) | 2493, 2793 |
| PagI | TCATGA | 1 cut(s) | 2322 |
| PaqCI | CACCTGC | 2 cut(s) | 1232, 1859 |
| PciI | ACATGT | 1 cut(s) | 1975 |
| PciSI | GCTCTTC | 1 cut(s) | 1841 |
| PdiI | GCCGGC | 1 cut(s) | 1168 |
| PdmI | GAANNNNTTC | 2 cut(s) | 518, 2753 |
| PfeI | GAWTC | 4 cut(s) | 134, 934, 1025, 2848 |
| PflFI | GACNNNGTC | 1 cut(s) | 1412 |
| PfoI | TCCNGGA | 1 cut(s) | 1255 |
| PleI | GAGTC | 3 cut(s) | 130, 938, 2363 |
| PpsI | GAGTC | 3 cut(s) | 130, 938, 2363 |
| Ppu21I | YACGTR | 1 cut(s) | 2664 |
| PpuMI | RGGWCCY | 2 cut(s) | 1055, 1482 |
| PscI | ACATGT | 1 cut(s) | 1975 |
| PshBI | ATTAAT | 1 cut(s) | 789 |
| PsiI | TTATAA | 1 cut(s) | 2454 |
| Psp124BI | GAGCTC | 1 cut(s) | 1774 |
| Psp5II | RGGWCCY | 2 cut(s) | 1055, 1482 |
| Psp6I | CCWGG | 5 cut(s) | 421, 1255, 1546, 2134, 2656 |
| PspFI | CCCAGC | 3 cut(s) | 1051, 2299, 2581 |
| PspGI | CCWGG | 5 cut(s) | 421, 1255, 1546, 2134, 2656 |
| PspN4I | GGNNCC | 9 cut(s) | 62, 843, 1056, 1288, 1483, 2088, 2297, 2439, 2685 |
| PspPI | GGNCC | 8 cut(s) | 665, 1055, 1169, 1482, 1538, 2087, 2174, 2437 |
| PspPPI | RGGWCCY | 2 cut(s) | 1055, 1482 |
| PspXI | VCTCGAGB | 2 cut(s) | 2493, 2793 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 426, 458 |
| PstI | CTGCAG | 2 cut(s) | 1243, 1717 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1874 |
| PsuI | RGATCY | 1 cut(s) | 841 |
| PsyI | GACNNNGTC | 1 cut(s) | 1412 |
| PvuII | CAGCTG | 1 cut(s) | 1526 |
| RsaI | GTAC | 3 cut(s) | 418, 644, 2666 |
| RsaNI | GTAC | 3 cut(s) | 417, 643, 2665 |
| RseI | CAYNNNNRTG | 5 cut(s) | 307, 660, 789, 1511, 2234 |
| SacI | GAGCTC | 1 cut(s) | 1774 |
| SapI | GCTCTTC | 1 cut(s) | 1841 |
| Sau96I | GGNCC | 8 cut(s) | 665, 1055, 1169, 1482, 1538, 2087, 2174, 2437 |
| SchI | GAGTC | 3 cut(s) | 131, 938, 2363 |
| SduI | GDGCHC | 7 cut(s) | 65, 259, 655, 1774, 1941, 2300, 2494 |
| SfaNI | GCATC | 7 cut(s) | 281, 1384, 1708, 1717, 1723, 1745, 2352 |
| SfcI | CTRYAG | 3 cut(s) | 1239, 1713, 1875 |
| Sfr274I | CTCGAG | 2 cut(s) | 2493, 2793 |
| SfuI | TTCGAA | 1 cut(s) | 553 |
| SinI | GGWCC | 5 cut(s) | 665, 1055, 1482, 1538, 2437 |
| SlaI | CTCGAG | 2 cut(s) | 2493, 2793 |
| SmaI | CCCGGG | 1 cut(s) | 2442 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 307, 660, 789, 1511, 2234 |
| SmlI | CTYRAG | 4 cut(s) | 733, 1308, 2493, 2793 |
| SmoI | CTYRAG | 4 cut(s) | 733, 1308, 2493, 2793 |
| SphI | GCATGC | 2 cut(s) | 1855, 2244 |
| SsiI | CCGC | 2 cut(s) | 66, 2060 |
| SspI | AATATT | 1 cut(s) | 288 |
| SstI | GAGCTC | 1 cut(s) | 1774 |
| StyI | CCWWGG | 4 cut(s) | 141, 1293, 1571, 1781 |
| TaaI | ACNGT | 5 cut(s) | 1195, 1412, 1555, 1585, 2623 |
| TaiI | ACGT | 1 cut(s) | 2666 |
| TaqI | TCGA | 9 cut(s) | 553, 690, 1368, 1416, 1606, 1776, 1816, 2494, 2794 |
| TaqII | GACCGA | 1 cut(s) | 20 |
| TatI | WGTACW | 1 cut(s) | 642 |
| TfiI | GAWTC | 4 cut(s) | 134, 934, 1025, 2848 |
| TscAI | CASTG | 3 cut(s) | 1198, 1714, 1747 |
| TseFI | GTSAC | 3 cut(s) | 1199, 1406, 2617 |
| Tsp45I | GTSAC | 3 cut(s) | 1199, 1406, 2617 |
| TspDTI | ATGAA | 6 cut(s) | 1059, 1097, 1848, 2067, 2370, 2520 |
| TspMI | CCCGGG | 1 cut(s) | 2440 |
| TspRI | CASTG | 3 cut(s) | 1198, 1714, 1747 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1412 |
| VpaK11BI | GGWCC | 5 cut(s) | 665, 1055, 1482, 1538, 2437 |
| VspI | ATTAAT | 1 cut(s) | 789 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 869 |
| XapI | RAATTY | 8 cut(s) | 244, 491, 518, 703, 904, 1755, 2479, 2726 |
| XbaI | TCTAGA | 1 cut(s) | 395 |
| XceI | RCATGY | 3 cut(s) | 1855, 1979, 2244 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2008 |
| XhoI | CTCGAG | 2 cut(s) | 2493, 2793 |
| XmaI | CCCGGG | 1 cut(s) | 2440 |
| XmaJI | CCTAGG | 1 cut(s) | 1781 |
| XmnI | GAANNNNTTC | 2 cut(s) | 518, 2753 |
| Zsp2I | ATGCAT | 3 cut(s) | 274, 1738, 2806 |