FvH4_3g42350

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000923
Physical Location & Seq
Reverse (-)
35357548 .. 35361466
3919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42350.t1

Sequence Viewer

Length: 2343 bp
ATGGGACATTCTTCACTTCTCCCTCCTTTTCTTCTCTCTGTTCTTATCCTCTCTTTGTTTCAGTCGCCGGCGGATGCAGCCAAACAGTCTTACATTGTGTACTTGGGAGCACATTCCCATGGCCTGAACCCTACGTCTGTCGAACTCGACGCCGCAACGACTTCTCACTACGATTTGCTTGGAACTGTTTTGGGAAGCAATGAGAGGGCCCAAGAAGCAATGTTTTACTCCTATACTGGTCAGATCAATGGCTTTGCTGCAGTTTTAGATGAAGAAGAGGCTTCACAGATTGCAAAGGATCCAAATGTTGTGTCTGTTTTTCCAAACAGAAAACTAAAACTACATACAACTCATTCATGGGAGTTTATGGGATTAGAAGACAAAGTTGGATTACCTCGTCACGGTTCGCTTTGGAAGAAAGCAAAGTTTGCTGAAAATACAATCATTGGAAACCTTGACACTGGTGTCTGGCCGGAATCAGAGAGCTTTAACGATCAAGGCTTTGGACCAATCCCATCCAAGTGGCGTGGAGCTTGTCACTTTGACACCAAAGACAAAGTTCGTTGCAACAGGAAGCTGATCGGAGCAAGATACTTCAACAAAGGATACTTGGCGTATGCGAAACTCAATAATGCCACCATCCCACCCAATGTTATGGTCTCGCCACGCGACCAGGAAGGCCATGGCTCACATACCCTTTCGACAGCTGGTGGCAGTTTTGTGCATGGGGCTAATGTTTTTGGTCATGGCAATGGTACTGCTAAAGGTGGATCCCCGAAAGCTCGTGTTGCTGCTTACAAAGTATGCTGGCCAAGTTTTCCTGGCACTACAGAAGGAGGGTGTTTCACTGCAGACATCCTGGCCGCCTTTGATGCTGCAATCAGTGATGGTGTTGATGTTCTTTCCTTGTCTGTTGGTGGTGATGCTGTAGAGTTTTCTGAAGATGGGATCGCAATAGGGTCATTCCATGCGGATAAGAATGGAATTGTTGTGGTGTGCTCAGCTGGAAATTCAGGACCTGCTCCGAGTACAGTGTCTAATGTCGCACCCTGGATGTTAACAGTTGGAGCTAGCACCCTTGATCGTGAGTTCTCTAGTTACATATCCTTCGGAAAGGGGAAGCATATGAAGGGAAAAAGTCTTTCATCAAGAAGTTTGCCGTCTAGAAAGTTTTACCCTGTGATAAGCGGAGCCAATGCTAATGCAGCTACTGCGAGCCCTGAAGATTCCTTGCTATGTTTGAATGGAACCCTCGATCCAAAGAAGGTGAAGGGAAAAATCTTGGTCTGCCAACGTGGGAAAAATGCGAGAACAGAAAAGGGCGAGATGGCAGCTCTTGCTGGGGCAGTTGGAATGGTTTTGGTTAACGATGAAGCCAGTGGAAATGAAATTATAGCTGATCCTCATGTGCTGCCGGCTACACATCTTACTTACGCTGATGGAAAACGTGTCTTTCAGTACCTGAATTCTACCAAGACCCCTTTTGCTCACATTACTCCTGTCAAGACGGTATACGGAACAAAGCCAGCTCCATTTATGGCTGCATTCTCATCTCGGGGACCAAGTGCCATTGAGCCATCAATACTTAAGCCTGATGTCACAGCACCAGGGGTGGATATAATTGCTGCCTATACAGGAGCAGCTGGTCCAACTGAATCAAAGTCTGATAAACGTCGCCCACGTTACATTACAGAATCTGGAACTTCTATGTCATGCCCTCATGTATCTGGAATCGTCGGTCTTCTGAAAACACTCTACCCGTCATGGAGCCCTGCCGCTATTAGATCTGCAATCATAACCACAGCAAGAAGACGCGACAACAACGAGGAGTCAATTCTGGATTCAACCAAGGAGCAGGCTACACCACTTGCTTATGGTGCAGGCCATGTCAATCCAAATCGTGCAGCAAACCCGGGACTTGTCTATGACTTGACTACCGATGATTACTTGAATTTCTTATGTGCTCGTGGATACAATGCATCACTTCTCAAACTATTTTCCGATAAGCCAGTCAAATGTTCCAACACCTCTAGCATTGCTGATCTGAACTATCCTACAATTGCAATTCCTGATCTTCAAGACAAACCAATCACCGTTACCAGAAGAGTGAAGAATGTTGGCACTCCAGGCACGTATGTCGCACATGTTCATACACCAGCTGGAGTTTCTATTTCGGTTAAGCCTCAGAAGTTGGAATTCAAGTCTGTTGGTGAAGAGAAGGAATTCAAGGTTACGCTGAAGGCGAATGGTAAGGTTGGAAATCCAGACTATGTGTTTGGAGAATTGAAATGGTCGGATGGCAAGCACAGTGTGAGGAGTCCCATTGCTGTGATGCGAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

781

Amino Acids

83.15

Weight (kDa)

8.9

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 30 - 115 9.3e-18 Peptidase inhibitor I9
MORF_dom PF21864 57 - 114 8e-07 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 146 - 626 5.5e-40 Subtilase family
PA PF02225 407 - 483 1.9e-15 PA domain
fn3_6 PF17766 681 - 777 3.7e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 1027
AccI GTMKAC 1 cut(s) 1512
AccII CGCG 2 cut(s) 669, 1815
AciI CCGC 6 cut(s) 71, 153, 864, 971, 1188, 1776
AclWI GGATC 7 cut(s) 293, 306, 765, 778, 956, 1250, 1394
AcoI YGGCCR 3 cut(s) 470, 809, 861
AcsI RAATTY 5 cut(s) 1009, 1465, 1951, 2195, 2222
AcuI CTGAAG 3 cut(s) 960, 1242, 2258
AcyI GRCGYC 1 cut(s) 150
AdeI CACNNNGTG 1 cut(s) 2311
AfaI GTAC 4 cut(s) 101, 757, 1030, 1460
AfiI CCNNNNNNNGG 1 cut(s) 401
AflII CTTAAG 1 cut(s) 1586
AflIII ACRYGT 2 cut(s) 1447, 2143
AgsI TTSAA 8 cut(s) 598, 1243, 1845, 1951, 2078, 2200, 2227, 2287
AjnI CCWGG 6 cut(s) 672, 820, 858, 1049, 1606, 2125
AjuI GAANNNNNNNTTGG 2 cut(s) 369, 401
AloI GAACNNNNNNTCC 2 cut(s) 1240, 1272
Alw21I GWGCWC 3 cut(s) 112, 1001, 1966
Alw26I GTCTC 1 cut(s) 664
AlwI GGATC 7 cut(s) 293, 306, 765, 778, 956, 1250, 1394
AlwNI CAGNNNCTG 4 cut(s) 1019, 1211, 1462, 1697
Ama87I CYCGRG 2 cut(s) 1554, 1912
AoxI GGCC 7 cut(s) 121, 207, 470, 679, 809, 861, 1882
ApaI GGGCCC 1 cut(s) 211
ApoI RAATTY 5 cut(s) 1009, 1465, 1951, 2195, 2222
ArsI GACNNNNNNTTYG 4 cut(s) 296, 328, 2258, 2290
Asp700I GAANNNNTTC 1 cut(s) 2222
AspS9I GGNCC 6 cut(s) 207, 208, 506, 1016, 1559, 1646
AsuC2I CCSGG 2 cut(s) 1913, 1914
AsuHPI GGTGA 4 cut(s) 932, 1279, 2083, 2222
AsuNHI GCTAGC 1 cut(s) 1070
AvaI CYCGRG 2 cut(s) 1554, 1912
AvaII GGWCC 4 cut(s) 506, 1016, 1559, 1646
BaeGI GKGCMC 1 cut(s) 211
BalI TGGCCA 1 cut(s) 811
BamHI GGATCC 2 cut(s) 298, 770
BanII GRGCYC 3 cut(s) 211, 1220, 1772
BauI CACGAG 2 cut(s) 783, 1965
BbsI GAAGAC 3 cut(s) 384, 1733, 1816
Bbv12I GWGCWC 3 cut(s) 112, 1001, 1966
BccI CCATC 8 cut(s) 523, 647, 881, 938, 1321, 1433, 1585, 2291
BceAI ACGGC 1 cut(s) 1144
BcgI CGANNNNNNTGC 2 cut(s) 2119, 2153
BciT130I CCWGG 6 cut(s) 674, 822, 860, 1051, 1608, 2127
BciVI GTATCC 2 cut(s) 599, 1964
BcnI CCSGG 2 cut(s) 1913, 1914
BcoDI GTCTC 1 cut(s) 664
BfaI CTAG 5 cut(s) 1071, 1095, 1164, 2031, 2341
BfmI CTRYAG 4 cut(s) 258, 828, 849, 927
BfrI CTTAAG 1 cut(s) 1586
BfuAI ACCTGC 1 cut(s) 1027
BfuI GTATCC 2 cut(s) 599, 1964
BglII AGATCT 1 cut(s) 1784
BlpI GCTNAGC 1 cut(s) 1000
Bme1390I CCNGG 8 cut(s) 674, 822, 860, 1051, 1608, 1913, 1914, 2127
Bme18I GGWCC 4 cut(s) 506, 1016, 1559, 1646
BmeT110I CYCGRG 2 cut(s) 1554, 1912
BmgT120I GGNCC 6 cut(s) 207, 208, 506, 1016, 1559, 1646
BmiI GGNNCC 7 cut(s) 209, 300, 772, 1192, 1249, 1560, 1769
BmrFI CCNGG 8 cut(s) 674, 822, 860, 1051, 1608, 1913, 1914, 2127
BmsI GCATC 5 cut(s) 64, 862, 913, 1988, 2322
BmtI GCTAGC 1 cut(s) 1074
BpiI GAAGAC 3 cut(s) 384, 1733, 1816
BpmI CTGGAG 2 cut(s) 2109, 2181
Bpu1102I GCTNAGC 1 cut(s) 1000
BpuMI CCSGG 2 cut(s) 1913, 1914
BsaAI YACGTR 1 cut(s) 2133
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 664
BsaJI CCNNGG 6 cut(s) 118, 682, 1049, 1607, 1848, 1912
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 2 cut(s) 67, 1414
Bse1I ACTGG 4 cut(s) 241, 466, 1377, 2009
Bse3DI GCAATG 5 cut(s) 205, 225, 757, 2034, 2322
BseBI CCWGG 6 cut(s) 674, 822, 860, 1051, 1608, 2127
BseDI CCNNGG 6 cut(s) 118, 682, 1049, 1607, 1848, 1912
BseGI GGATG 6 cut(s) 79, 515, 639, 855, 1059, 2302
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 5 cut(s) 205, 225, 757, 2034, 2322
BseMII CTCAG 2 cut(s) 1014, 2198
BseNI ACTGG 4 cut(s) 241, 466, 1377, 2009
BseRI GAGGAG 2 cut(s) 1841, 2329
BseSI GKGCMC 1 cut(s) 211
BseYI CCCAGC 1 cut(s) 1340
BsgI GTGCAG 2 cut(s) 1899, 1923
Bsh1236I CGCG 2 cut(s) 669, 1815
BshFI GGCC 7 cut(s) 123, 209, 472, 681, 811, 863, 1884
BsiHKAI GWGCWC 3 cut(s) 112, 1001, 1966
BsiHKCI CYCGRG 2 cut(s) 1554, 1912
BsiSI CCGG 4 cut(s) 68, 473, 1415, 1913
BslFI GGGAC 4 cut(s) 18, 1572, 1929, 2304
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 664
BsmFI GGGAC 4 cut(s) 18, 1572, 1929, 2304
BsmI GAATGC 1 cut(s) 1544
BsnI GGCC 7 cut(s) 123, 209, 472, 681, 811, 863, 1884
Bso31I GGTCTC 1 cut(s) 664
BsoBI CYCGRG 2 cut(s) 1554, 1912
Bsp120I GGGCCC 1 cut(s) 207
Bsp1286I GDGCHC 6 cut(s) 112, 211, 1001, 1220, 1772, 1966
Bsp1720I GCTNAGC 1 cut(s) 1000
Bsp19I CCATGG 2 cut(s) 118, 682
BspACI CCGC 6 cut(s) 71, 153, 864, 971, 1188, 1776
BspANI GGCC 7 cut(s) 123, 209, 472, 681, 811, 863, 1884
BspCNI CTCAG 2 cut(s) 1013, 2197
BspFNI CGCG 2 cut(s) 669, 1815
BspLI GGNNCC 7 cut(s) 209, 300, 772, 1192, 1249, 1560, 1769
BspMAI CTGCAG 2 cut(s) 262, 853
BspMI ACCTGC 1 cut(s) 1027
BspOI GCTAGC 1 cut(s) 1074
BspPI GGATC 7 cut(s) 293, 306, 765, 778, 956, 1250, 1394
BspTI CTTAAG 1 cut(s) 1586
BspTNI GGTCTC 1 cut(s) 664
BsrDI GCAATG 5 cut(s) 205, 225, 757, 2034, 2322
BsrFI RCCGGY 2 cut(s) 67, 1414
BsrI ACTGG 4 cut(s) 241, 466, 1377, 2009
BssAI RCCGGY 2 cut(s) 67, 1414
BssECI CCNNGG 6 cut(s) 118, 682, 1049, 1607, 1848, 1912
BssNAI GTATAC 1 cut(s) 1513
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 2 cut(s) 783, 1965
BssT1I CCWWGG 3 cut(s) 118, 682, 1848
Bst1107I GTATAC 1 cut(s) 1513
Bst2BI CACGAG 2 cut(s) 783, 1965
Bst2UI CCWGG 6 cut(s) 674, 822, 860, 1051, 1608, 2127
Bst4CI ACNGT 8 cut(s) 87, 187, 404, 1033, 1063, 1510, 2095, 2309
Bst6I CTCTTC 3 cut(s) 270, 2098, 2208
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 1586
BstAPI GCANNNNNTGC 3 cut(s) 428, 1211, 1337
BstBAI YACGTR 1 cut(s) 2133
BstC8I GCNNGC 9 cut(s) 69, 809, 1072, 1216, 1416, 1527, 1857, 1882, 2303
BstDEI CTNAG 2 cut(s) 1000, 2184
BstDSI CCRYGG 2 cut(s) 118, 682
BstF5I GGATG 6 cut(s) 79, 515, 639, 855, 1059, 2302
BstFNI CGCG 2 cut(s) 669, 1815
BstMAI GTCTC 1 cut(s) 664
BstNI CCWGG 6 cut(s) 674, 822, 860, 1051, 1608, 2127
BstNSI RCATGY 1 cut(s) 2147
BstSCI CCNGG 8 cut(s) 672, 820, 858, 1049, 1606, 1911, 1912, 2125
BstSFI CTRYAG 4 cut(s) 258, 828, 849, 927
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 2 cut(s) 669, 1815
BstV2I GAAGAC 3 cut(s) 384, 1733, 1816
BstX2I RGATCY 3 cut(s) 298, 770, 1784
BstXI CCANNNNNNTGG 2 cut(s) 522, 655
BstYI RGATCY 3 cut(s) 298, 770, 1784
BstZ17I GTATAC 1 cut(s) 1513
BsuI GTATCC 2 cut(s) 599, 1964
BsuRI GGCC 7 cut(s) 123, 209, 472, 681, 811, 863, 1884
BtgI CCRYGG 2 cut(s) 118, 682
BtsCI GGATG 6 cut(s) 79, 515, 639, 855, 1059, 2302
BtsI GCAGTG 1 cut(s) 846
BtsIMutI CAGTG 6 cut(s) 459, 846, 889, 1038, 1384, 2314
BveI ACCTGC 1 cut(s) 1027
Cac8I GCNNGC 9 cut(s) 69, 809, 1072, 1216, 1416, 1527, 1857, 1882, 2303
CaiI CAGNNNCTG 4 cut(s) 1019, 1211, 1462, 1697
Cfr10I RCCGGY 2 cut(s) 67, 1414
Cfr13I GGNCC 6 cut(s) 207, 208, 506, 1016, 1559, 1646
Cfr9I CCCGGG 1 cut(s) 1912
CseI GACGC 2 cut(s) 158, 1821
Csp6I GTAC 4 cut(s) 100, 756, 1029, 1459
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviQI GTAC 4 cut(s) 100, 756, 1029, 1459
DdeI CTNAG 2 cut(s) 1000, 2184
DraIII CACNNNGTG 1 cut(s) 2311
DrdI GACNNNNNNGTC 1 cut(s) 464
DseDI GACNNNNNNGTC 1 cut(s) 464
EaeI YGGCCR 3 cut(s) 470, 809, 861
Eam1104I CTCTTC 3 cut(s) 270, 2098, 2208
EarI CTCTTC 3 cut(s) 270, 2098, 2208
EciI GGCGGA 1 cut(s) 86
Eco130I CCWWGG 3 cut(s) 118, 682, 1848
Eco24I GRGCYC 3 cut(s) 211, 1220, 1772
Eco31I GGTCTC 1 cut(s) 664
Eco47I GGWCC 4 cut(s) 506, 1016, 1559, 1646
Eco57I CTGAAG 3 cut(s) 960, 1242, 2258
Eco88I CYCGRG 2 cut(s) 1554, 1912
EcoO109I RGGNCCY 2 cut(s) 207, 1016
EcoRI GAATTC 3 cut(s) 1465, 2195, 2222
EcoRII CCWGG 6 cut(s) 672, 820, 858, 1049, 1606, 2125
EcoT14I CCWWGG 3 cut(s) 118, 682, 1848
EcoT22I ATGCAT 1 cut(s) 1981
EcoT38I GRGCYC 3 cut(s) 211, 1220, 1772
ErhI CCWWGG 3 cut(s) 118, 682, 1848
FaqI GGGAC 4 cut(s) 18, 1572, 1929, 2304
FauNDI CATATG 1 cut(s) 1125
FblI GTMKAC 1 cut(s) 1512
FokI GGATG 6 cut(s) 86, 502, 626, 842, 1066, 2309
FriOI GRGCYC 3 cut(s) 211, 1220, 1772
FspBI CTAG 5 cut(s) 1071, 1095, 1164, 2031, 2341
GsaI CCCAGC 1 cut(s) 1344
GsuI CTGGAG 2 cut(s) 2109, 2181
HaeIII GGCC 7 cut(s) 123, 209, 472, 681, 811, 863, 1884
HapII CCGG 4 cut(s) 68, 473, 1415, 1913
HgaI GACGC 2 cut(s) 158, 1821
Hin1I GRCGYC 1 cut(s) 150
HincII GTYRAC 2 cut(s) 1059, 1366
HindII GTYRAC 2 cut(s) 1059, 1366
HinfI GANTC 8 cut(s) 476, 1226, 1655, 1694, 1731, 1829, 1841, 2317
HpaI GTTAAC 2 cut(s) 1059, 1366
HpaII CCGG 4 cut(s) 68, 473, 1415, 1913
HphI GGTGA 4 cut(s) 932, 1279, 2083, 2222
Hpy166II GTNNAC 4 cut(s) 100, 1059, 1366, 1513
Hpy8I GTNNAC 4 cut(s) 100, 1059, 1366, 1513
Hpy99I CGWCG 3 cut(s) 152, 1677, 1739
HpyAV CCTTC 8 cut(s) 671, 827, 1117, 1123, 1258, 1264, 2212, 2233
HpyCH4III ACNGT 8 cut(s) 87, 187, 404, 1033, 1063, 1510, 2095, 2309
HpyCH4IV ACGT 6 cut(s) 134, 1294, 1447, 1672, 1681, 2132
HpyF3I CTNAG 2 cut(s) 1000, 2184
HpySE526I ACGT 6 cut(s) 134, 1294, 1447, 1672, 1681, 2132
Hsp92I GRCGYC 1 cut(s) 150
KroI GCCGGC 2 cut(s) 67, 1414
KroNI GCCGGC 2 cut(s) 69, 1416
KspAI GTTAAC 2 cut(s) 1059, 1366
LweI GCATC 5 cut(s) 64, 862, 913, 1988, 2322
MaeI CTAG 5 cut(s) 1071, 1095, 1164, 2031, 2341
MaeII ACGT 6 cut(s) 134, 1294, 1447, 1672, 1681, 2132
MaeIII GTNAC 7 cut(s) 398, 536, 1097, 1597, 1682, 2095, 2230
MfeI CAATTG 1 cut(s) 2058
MflI RGATCY 3 cut(s) 298, 770, 1784
MhlI GDGCHC 6 cut(s) 112, 211, 1001, 1220, 1772, 1966
MlsI TGGCCA 1 cut(s) 811
MluNI TGGCCA 1 cut(s) 811
MlyI GAGTC 2 cut(s) 1838, 2326
MmeI TCCRAC 8 cut(s) 367, 1045, 1330, 1673, 2046, 2172, 2236, 2274
Mox20I TGGCCA 1 cut(s) 811
Mph1103I ATGCAT 1 cut(s) 1981
MreI CGCCGGCG 1 cut(s) 67
MroNI GCCGGC 2 cut(s) 67, 1414
MroXI GAANNNNTTC 1 cut(s) 2222
MscI TGGCCA 1 cut(s) 811
MseI TTAA 5 cut(s) 489, 1058, 1365, 1587, 2178
MslI CAYNNNNRTG 4 cut(s) 117, 520, 750, 2327
Msp20I TGGCCA 1 cut(s) 811
MspA1I CMGCKG 4 cut(s) 707, 1004, 1643, 2159
MspCI CTTAAG 1 cut(s) 1586
MspI CCGG 4 cut(s) 68, 473, 1415, 1913
MspR9I CCNGG 8 cut(s) 674, 822, 860, 1051, 1608, 1913, 1914, 2127
MunI CAATTG 1 cut(s) 2058
Mva1269I GAATGC 1 cut(s) 1544
MvaI CCWGG 6 cut(s) 674, 822, 860, 1051, 1608, 2127
MvnI CGCG 2 cut(s) 669, 1815
NaeI GCCGGC 2 cut(s) 69, 1416
NciI CCSGG 2 cut(s) 1913, 1914
NcoI CCATGG 2 cut(s) 118, 682
NdeI CATATG 1 cut(s) 1125
NgoMIV GCCGGC 2 cut(s) 67, 1414
NheI GCTAGC 1 cut(s) 1070
NlaIV GGNNCC 7 cut(s) 209, 300, 772, 1192, 1249, 1560, 1769
NmuCI GTSAC 3 cut(s) 398, 536, 1597
NsiI ATGCAT 1 cut(s) 1981
NspI RCATGY 1 cut(s) 2147
PciI ACATGT 1 cut(s) 2143
PcsI WCGNNNNNNNCGW 2 cut(s) 1678, 2240
PctI GAATGC 1 cut(s) 1544
PdiI GCCGGC 2 cut(s) 69, 1416
PdmI GAANNNNTTC 1 cut(s) 2222
PfeI GAWTC 6 cut(s) 476, 1226, 1655, 1694, 1731, 1841
PleI GAGTC 2 cut(s) 1837, 2325
PpsI GAGTC 2 cut(s) 1837, 2325
Ppu21I YACGTR 1 cut(s) 2133
PpuMI RGGWCCY 1 cut(s) 1016
PscI ACATGT 1 cut(s) 2143
Psp5II RGGWCCY 1 cut(s) 1016
Psp6I CCWGG 6 cut(s) 672, 820, 858, 1049, 1606, 2125
PspFI CCCAGC 1 cut(s) 1340
PspGI CCWGG 6 cut(s) 672, 820, 858, 1049, 1606, 2125
PspN4I GGNNCC 7 cut(s) 209, 300, 772, 1192, 1249, 1560, 1769
PspOMI GGGCCC 1 cut(s) 207
PspPI GGNCC 6 cut(s) 207, 208, 506, 1016, 1559, 1646
PspPPI RGGWCCY 1 cut(s) 1016
PstI CTGCAG 2 cut(s) 262, 853
PstNI CAGNNNCTG 4 cut(s) 1019, 1211, 1462, 1697
PsuI RGATCY 3 cut(s) 298, 770, 1784
PvuII CAGCTG 4 cut(s) 707, 1004, 1643, 2159
RsaI GTAC 4 cut(s) 101, 757, 1030, 1460
RsaNI GTAC 4 cut(s) 100, 756, 1029, 1459
RseI CAYNNNNRTG 4 cut(s) 117, 520, 750, 2327
SaqAI TTAA 5 cut(s) 489, 1058, 1365, 1587, 2178
Sau96I GGNCC 6 cut(s) 207, 208, 506, 1016, 1559, 1646
SchI GAGTC 2 cut(s) 1838, 2326
ScrFI CCNGG 8 cut(s) 674, 822, 860, 1051, 1608, 1913, 1914, 2127
SduI GDGCHC 6 cut(s) 112, 211, 1001, 1220, 1772, 1966
SfaNI GCATC 5 cut(s) 64, 862, 913, 1988, 2322
SfcI CTRYAG 4 cut(s) 258, 828, 849, 927
SgrAI CRCCGGYG 1 cut(s) 67
SinI GGWCC 4 cut(s) 506, 1016, 1559, 1646
SmaI CCCGGG 1 cut(s) 1914
SmiMI CAYNNNNRTG 4 cut(s) 117, 520, 750, 2327
SmlI CTYRAG 1 cut(s) 1586
SmoI CTYRAG 1 cut(s) 1586
SsiI CCGC 6 cut(s) 71, 153, 864, 971, 1188, 1776
SspMI CTAG 5 cut(s) 1071, 1095, 1164, 2031, 2341
StyD4I CCNGG 8 cut(s) 672, 820, 858, 1049, 1606, 1911, 1912, 2125
StyI CCWWGG 3 cut(s) 118, 682, 1848
TaaI ACNGT 8 cut(s) 87, 187, 404, 1033, 1063, 1510, 2095, 2309
TaiI ACGT 6 cut(s) 137, 1297, 1450, 1675, 1684, 2135
TaqI TCGA 4 cut(s) 141, 147, 701, 1254
TaqII GACCGA 1 cut(s) 1727
TatI WGTACW 2 cut(s) 99, 1028
TauI GCSGC 3 cut(s) 155, 866, 1778
TfiI GAWTC 6 cut(s) 476, 1226, 1655, 1694, 1731, 1841
Tru1I TTAA 5 cut(s) 489, 1058, 1365, 1587, 2178
Tru9I TTAA 5 cut(s) 489, 1058, 1365, 1587, 2178
TscAI CASTG 6 cut(s) 466, 853, 889, 1038, 1384, 2314
TseFI GTSAC 3 cut(s) 398, 536, 1597
Tsp45I GTSAC 3 cut(s) 398, 536, 1597
TspDTI ATGAA 7 cut(s) 285, 345, 1134, 1142, 1386, 1401, 2138
TspGWI ACGGA 1 cut(s) 1530
TspMI CCCGGG 1 cut(s) 1912
TspRI CASTG 6 cut(s) 466, 853, 889, 1038, 1384, 2314
Vha464I CTTAAG 1 cut(s) 1586
VpaK11BI GGWCC 4 cut(s) 506, 1016, 1559, 1646
XapI RAATTY 5 cut(s) 1009, 1465, 1951, 2195, 2222
XbaI TCTAGA 1 cut(s) 1163
XceI RCATGY 1 cut(s) 2147
XcmI CCANNNNNNNNNTGG 1 cut(s) 680
XmaI CCCGGG 1 cut(s) 1912
XmiI GTMKAC 1 cut(s) 1512
XmnI GAANNNNTTC 1 cut(s) 2222
XspI CTAG 5 cut(s) 1071, 1095, 1164, 2031, 2341
Zsp2I ATGCAT 1 cut(s) 1981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.