Prupe.6G031500_v2.0.a1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000923
Physical Location & Seq
Forward (+)
2443556 .. 2448841
5286 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G031500.5

Sequence Viewer

Length: 2319 bp
ATGGTGCTTTTAACTCTTCCTTCCTTGCTTCTACCAGTTCTTTTGTTCTTTCTGTTGCAGACACCTACCCTATCCATAAAAAAGTCATTCATAGTGTACTTAGGAGCACATTCACATGGCCCCGACCCCTCATCCGTGGATCTCGATTCTGTGAGAAAATTTCATTATGATTTTCTGGGGTCTTTCTTGAGAAGCAATAAGAGTGCCAAAGATGTGATCTTCTACTCCTACACTAGACATATTAATGGTTTTGCTGCAATTCTTGAAGAGGAAGAAGCTGCAGATATTGCAGAGCATCCAAATGTGATATCGGTTTTCTTAAACAAGGGAAGCAAACTGGAAACAACTAGATCATGGAATTTTCTTGGATTGGAGAGAAATGGACTAATTCCTTCATACTCCATTTGGATGAAGGCAAGGTTGGGAGAAGATACAATAATTGCAAACATAGATACTGGTGTTTGGCCAGAATCAAAAAGCTTTAGTGATGAAGGGTTGGGACCCGTCCCTTCAAAGTGGCGTGGAATTTGTCAACATGATACAAAGCGAGTTCGTTGTAACAGAAAGCTGATTGGGACAAGGTACTTTAACAATGGTCTTGCCATGTATGCTGGCCCTCTCAATTCCTCCTTTTCCACCGCTCGTGACTATGATGGCCATGGATCCCACACCCTTGCCACGGCTGCTGGCAACTTTGTTCCCGGAGTAAGTGTTTTTGGAAATGGCAATGGAACTGCCAAAGGTGGATCGCCTCGAGCCCGCGTTGCTGCGTATAAGGTATGCTGGGCACCATATGAAGGTGTCCAGTGCTTTGATGCAGACGTATTAGCTGCCTTTGATGCTGCAATAAGTGATGGAGTCGATATAATCTCTGTGTCTCTTGGTGGAGGAGCACAAGAATTTTTTAAAAATTCAATTTCAATAGGGGCCTTCCATGCAGTTAAGCATGGCATTGTTGTGGTTAGTGCAGCTGGCAATTCAGGACCAAATCCAGGGACAGTGTTAAATCTGTCGCCCTGGTTATTAACAGTTGGTGCTGGCACGATCGATCGGGAGTTCACAAGTTATGTCTCTCTTGGAAACAAGAAGCATTTGAAGGGAGTTAGCCTTTCAGCTAAAGGCTTGCCATCGGAAAAGTTCTACCCATTGGTCAGCGCAGCAGAAGCGAAACATGCTAATGCATCCACCGCAGAAGCAATAATTTGCCAGGGTGGAACCCTTGATCCTAGGAAGGTGAAAGGAAAGATCTTGGTTTGCCTTCAAGAATATAACGATAATGCAAGAACTGAGAAGAGCTGGCAGGCTGATATGGCAGGTGCTGTAGGAATGATCTTGGTTAATGATGAGCAAAGTGGAAATGATGTTGTAGCCGATCCTCATGTGCTCCTTGTTTCACATGTCAACTACACTGACGGCAAATATATCTTTGATTACATTAAATCTACCAAAACCCCTATGGCTTACTTAACTCGAGTCAAGACTGAATTTGGATCAAAGCCAGCTCCATTTGTGGCTACATTTTCATCTAGGGGGCCTAACCTCCTGGAACAGGGAATCTTAAAGCCTGATATCATTGCACCAGGGGTGAGCATAATTGCTGCTTATACTGAAGCAGCAGGGCCAACTTCTCAAATTTCCGATACTCGCCGGGTTCCTTTTAATGTGCAAACTGGGAGTTCAATGGCATGCCCTCATGCATCTGGAATTGCAGGGCTTCTCAGGACACTCCACCCTGATTGGAGTCCGGCAGCTATTAAATCTGCAATCATGACCACTGCTACAACAGAGGATGACAGCATGGAACCAATACCTGATGACTCTTCTTATGTGAAGGCAACACCATTTGCTTATGGCTCAGGACACATTCAACCAAACAAAGCAATGGATCCGGGACTAGTTTACAACCTAACAACGCTAGATTACTTGAACTTCTTATGCGCTCGCGGATATAACGAAACCATGATCAAATCATTCTCCAACTCAATTTACAAGTGTTCCAAGTCATTCAGCCTAGCAGACTTCAACTACCCTTCAATTTCAGTTCCTAATCTCAGTGAAGACTCAGTGACCATAAACAGAAAAGTTACAAATGTTGGTTCTCCCGGCACATACAAAGTGCATGTCAAGGAGCCATCAGAAGTAGAAGTTTTGGTTAAACCTAGAAGATTAAAATTTAAGAGAATTGGTGAAGTGAAGATGTTCAAGGTTATATTGAAAGCTAAGGTTAAGGGTAAGCCTCAAGGGTATGTATTTGGAGAGTTGATATGGTCAGATGGCAGTCACTATGTAAAGAGCCCTCTTGCAGTGAAGCACTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

773

Amino Acids

83.57

Weight (kDa)

8.85

Isoelectric Point (pI)

30.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1304
Acc36I ACCTGC 1 cut(s) 1304
AccB1I GGYRCC 1 cut(s) 787
AccBSI CCGCTC 1 cut(s) 641
AccII CGCG 2 cut(s) 762, 1944
AciI CCGC 4 cut(s) 639, 760, 1188, 1944
AclWI GGATC 9 cut(s) 147, 657, 670, 754, 1217, 1367, 1498, 1880, 1893
AcoI YGGCCR 2 cut(s) 464, 655
AcsI RAATTY 8 cut(s) 158, 358, 525, 899, 910, 1484, 1632, 2171
AcuI CTGAAG 1 cut(s) 1629
AfaI GTAC 2 cut(s) 98, 584
AfiI CCNNNNNNNGG 4 cut(s) 679, 797, 992, 1549
AflIII ACRYGT 1 cut(s) 1396
AhlI ACTAGT 1 cut(s) 1894
AjnI CCWGG 5 cut(s) 991, 1016, 1206, 1542, 1579
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AloI GAACNNNNNNTCC 2 cut(s) 1207, 1239
Alw21I GWGCWC 3 cut(s) 109, 895, 1386
Alw26I GTCTC 2 cut(s) 882, 1075
AlwI GGATC 9 cut(s) 147, 657, 670, 754, 1217, 1367, 1498, 1880, 1893
AlwNI CAGNNNCTG 1 cut(s) 1319
Ama87I CYCGRG 2 cut(s) 753, 1470
AoxI GGCC 7 cut(s) 118, 464, 613, 655, 927, 1532, 1619
ApoI RAATTY 8 cut(s) 158, 358, 525, 899, 910, 1484, 1632, 2171
ArsI GACNNNNNNTTYG 2 cut(s) 2106, 2138
AseI ATTAAT 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 2198
AspA2I CCTAGG 1 cut(s) 1226
AspLEI GCGC 2 cut(s) 1157, 1940
AspS9I GGNCC 7 cut(s) 119, 500, 614, 927, 983, 1532, 1619
AsuC2I CCSGG 4 cut(s) 702, 1649, 1890, 2103
AsuHPI GGTGA 3 cut(s) 1246, 1597, 2198
AvaI CYCGRG 2 cut(s) 753, 1470
AvaII GGWCC 2 cut(s) 500, 983
AvrII CCTAGG 1 cut(s) 1226
BaeGI GKGCMC 1 cut(s) 790
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BalI TGGCCA 2 cut(s) 466, 657
BamHI GGATCC 2 cut(s) 662, 1885
BanI GGYRCC 1 cut(s) 787
BanII GRGCYC 2 cut(s) 760, 2297
BauI CACGAG 1 cut(s) 642
BbsI GAAGAC 1 cut(s) 2064
Bbv12I GWGCWC 3 cut(s) 109, 895, 1386
BccI CCATC 5 cut(s) 647, 848, 1135, 2140, 2267
BceAI ACGGC 2 cut(s) 696, 1429
BciT130I CCWGG 5 cut(s) 993, 1018, 1208, 1544, 1581
BclI TGATCA 1 cut(s) 1962
BcnI CCSGG 4 cut(s) 702, 1649, 1890, 2103
BcoDI GTCTC 2 cut(s) 882, 1075
BcuI ACTAGT 1 cut(s) 1894
BfaI CTAG 8 cut(s) 234, 348, 1227, 1527, 1895, 1916, 2012, 2160
BfmI CTRYAG 2 cut(s) 279, 1320
BfuAI ACCTGC 1 cut(s) 1304
BglII AGATCT 1 cut(s) 1245
BlnI CCTAGG 1 cut(s) 1226
Bme1390I CCNGG 9 cut(s) 702, 993, 1018, 1208, 1544, 1581, 1649, 1890, 2103
Bme18I GGWCC 2 cut(s) 500, 983
BmeT110I CYCGRG 2 cut(s) 753, 1470
BmgT120I GGNCC 7 cut(s) 119, 500, 614, 927, 983, 1532, 1619
BmrFI CCNGG 9 cut(s) 702, 993, 1018, 1208, 1544, 1581, 1649, 1890, 2103
BmrI ACTGGG 1 cut(s) 1680
BmsI GCATC 5 cut(s) 304, 805, 829, 1190, 1706
BmuI ACTGGG 1 cut(s) 1680
BpiI GAAGAC 1 cut(s) 2064
Bpu10I CCTNAGC 2 cut(s) 1855, 2220
BpuEI CTTGAG 2 cut(s) 208, 2223
BpuMI CCSGG 4 cut(s) 702, 1649, 1890, 2103
Bsa29I ATCGAT 1 cut(s) 1047
BsaJI CCNNGG 8 cut(s) 135, 658, 678, 992, 1016, 1207, 1226, 1580
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 4 cut(s) 679, 797, 992, 1549
Bse1I ACTGG 5 cut(s) 35, 342, 460, 805, 1675
Bse3DI GCAATG 3 cut(s) 733, 1572, 1887
BseBI CCWGG 5 cut(s) 993, 1018, 1208, 1544, 1581
BseCI ATCGAT 1 cut(s) 1047
BseDI CCNNGG 8 cut(s) 135, 658, 678, 992, 1016, 1207, 1226, 1580
BseGI GGATG 5 cut(s) 131, 295, 414, 1181, 1795
BseLI CCNNNNNNNGG 4 cut(s) 679, 797, 992, 1549
BseMI GCAATG 3 cut(s) 733, 1572, 1887
BseMII CTCAG 5 cut(s) 1278, 1732, 1869, 2065, 2076
BseNI ACTGG 5 cut(s) 35, 342, 460, 805, 1675
BseRI GAGGAG 1 cut(s) 903
BseSI GKGCMC 1 cut(s) 790
BseYI CCCAGC 1 cut(s) 783
BsgI GTGCAG 1 cut(s) 987
Bsh1236I CGCG 2 cut(s) 762, 1944
Bsh1285I CGRYCG 2 cut(s) 1047, 1051
BshFI GGCC 7 cut(s) 120, 466, 615, 657, 929, 1534, 1621
BshNI GGYRCC 1 cut(s) 787
BshVI ATCGAT 1 cut(s) 1047
BsiEI CGRYCG 2 cut(s) 1047, 1051
BsiHKAI GWGCWC 3 cut(s) 109, 895, 1386
BsiHKCI CYCGRG 2 cut(s) 753, 1470
BsiSI CCGG 5 cut(s) 702, 1648, 1745, 1889, 2103
BslFI GGGAC 5 cut(s) 491, 513, 589, 1009, 1905
BslI CCNNNNNNNGG 4 cut(s) 679, 797, 992, 1549
BsmAI GTCTC 2 cut(s) 882, 1075
BsmFI GGGAC 5 cut(s) 491, 513, 589, 1009, 1905
BsnI GGCC 7 cut(s) 120, 466, 615, 657, 929, 1534, 1621
BsoBI CYCGRG 2 cut(s) 753, 1470
Bsp1286I GDGCHC 6 cut(s) 109, 760, 790, 895, 1386, 2297
Bsp19I CCATGG 1 cut(s) 658
BspACI CCGC 4 cut(s) 639, 760, 1188, 1944
BspANI GGCC 7 cut(s) 120, 466, 615, 657, 929, 1534, 1621
BspCNI CTCAG 5 cut(s) 1279, 1731, 1868, 2064, 2075
BspDI ATCGAT 1 cut(s) 1047
BspFNI CGCG 2 cut(s) 762, 1944
BspHI TCATGA 1 cut(s) 1767
BspMAI CTGCAG 1 cut(s) 283
BspMI ACCTGC 1 cut(s) 1304
BspPI GGATC 9 cut(s) 147, 657, 670, 754, 1217, 1367, 1498, 1880, 1893
BspQI GCTCTTC 1 cut(s) 1286
BspT107I GGYRCC 1 cut(s) 787
BsrBI CCGCTC 1 cut(s) 641
BsrDI GCAATG 3 cut(s) 733, 1572, 1887
BsrI ACTGG 5 cut(s) 35, 342, 460, 805, 1675
BssECI CCNNGG 8 cut(s) 135, 658, 678, 992, 1016, 1207, 1226, 1580
BssSI CACGAG 1 cut(s) 642
BssT1I CCWWGG 2 cut(s) 658, 1226
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 5 cut(s) 993, 1018, 1208, 1544, 1581
Bst4CI ACNGT 2 cut(s) 1000, 1030
Bst6I CTCTTC 4 cut(s) 21, 261, 1286, 1825
BstAPI GCANNNNNTGC 1 cut(s) 287
BstDEI CTNAG 7 cut(s) 100, 1287, 1718, 1855, 2051, 2062, 2220
BstDSI CCRYGG 3 cut(s) 135, 658, 678
BstENI CCTNNNNNAGG 1 cut(s) 1547
BstF5I GGATG 5 cut(s) 131, 295, 414, 1181, 1795
BstFNI CGCG 2 cut(s) 762, 1944
BstHHI GCGC 2 cut(s) 1157, 1940
BstMAI GTCTC 2 cut(s) 882, 1075
BstMCI CGRYCG 2 cut(s) 1047, 1051
BstNI CCWGG 5 cut(s) 993, 1018, 1208, 1544, 1581
BstNSI RCATGY 4 cut(s) 1175, 1400, 1689, 2123
BstSCI CCNGG 9 cut(s) 700, 991, 1016, 1206, 1542, 1579, 1647, 1888, 2101
BstSFI CTRYAG 2 cut(s) 279, 1320
BstSLI GKGCMC 1 cut(s) 790
BstUI CGCG 2 cut(s) 762, 1944
BstV2I GAAGAC 1 cut(s) 2064
BstX2I RGATCY 4 cut(s) 139, 662, 1245, 1885
BstYI RGATCY 4 cut(s) 139, 662, 1245, 1885
Bsu15I ATCGAT 1 cut(s) 1047
BsuRI GGCC 7 cut(s) 120, 466, 615, 657, 929, 1534, 1621
BsuTUI ATCGAT 1 cut(s) 1047
BtgI CCRYGG 3 cut(s) 135, 658, 678
BtsCI GGATG 5 cut(s) 131, 295, 414, 1181, 1795
BtsI GCAGTG 2 cut(s) 1773, 2310
BtsIMutI CAGTG 7 cut(s) 812, 1005, 1407, 1773, 2059, 2070, 2310
BveI ACCTGC 1 cut(s) 1304
CaiI CAGNNNCTG 1 cut(s) 1319
CciI TCATGA 1 cut(s) 1767
CfoI GCGC 2 cut(s) 1157, 1940
Cfr13I GGNCC 7 cut(s) 119, 500, 614, 927, 983, 1532, 1619
ClaI ATCGAT 1 cut(s) 1047
Csp6I GTAC 2 cut(s) 97, 583
CspCI CAANNNNNGTGG 2 cut(s) 1718, 1753
CviQI GTAC 2 cut(s) 97, 583
DdeI CTNAG 7 cut(s) 100, 1287, 1718, 1855, 2051, 2062, 2220
DraI TTTAAA 1 cut(s) 907
EaeI YGGCCR 2 cut(s) 464, 655
Eam1104I CTCTTC 4 cut(s) 21, 261, 1286, 1825
EarI CTCTTC 4 cut(s) 21, 261, 1286, 1825
Eco130I CCWWGG 2 cut(s) 658, 1226
Eco24I GRGCYC 2 cut(s) 760, 2297
Eco32I GATATC 2 cut(s) 309, 1570
Eco47I GGWCC 2 cut(s) 500, 983
Eco57I CTGAAG 1 cut(s) 1629
Eco88I CYCGRG 2 cut(s) 753, 1470
EcoNI CCTNNNNNAGG 1 cut(s) 1547
EcoO109I RGGNCCY 3 cut(s) 500, 927, 1532
EcoRII CCWGG 5 cut(s) 991, 1016, 1206, 1542, 1579
EcoRV GATATC 2 cut(s) 309, 1570
EcoT14I CCWWGG 2 cut(s) 658, 1226
EcoT22I ATGCAT 2 cut(s) 1183, 1699
EcoT38I GRGCYC 2 cut(s) 760, 2297
ErhI CCWWGG 2 cut(s) 658, 1226
FaqI GGGAC 5 cut(s) 491, 513, 589, 1009, 1905
FauI CCCGC 1 cut(s) 767
FauNDI CATATG 1 cut(s) 793
FbaI TGATCA 1 cut(s) 1962
FokI GGATG 5 cut(s) 118, 282, 421, 1168, 1802
FriOI GRGCYC 2 cut(s) 760, 2297
FspBI CTAG 8 cut(s) 234, 348, 1227, 1527, 1895, 1916, 2012, 2160
GlaI GCGC 2 cut(s) 1156, 1939
GsaI CCCAGC 1 cut(s) 787
HaeIII GGCC 7 cut(s) 120, 466, 615, 657, 929, 1534, 1621
HapII CCGG 5 cut(s) 702, 1648, 1745, 1889, 2103
HhaI GCGC 2 cut(s) 1157, 1940
Hin6I GCGC 2 cut(s) 1155, 1938
HinP1I GCGC 2 cut(s) 1155, 1938
HincII GTYRAC 2 cut(s) 533, 1402
HindII GTYRAC 2 cut(s) 533, 1402
HindIII AAGCTT 1 cut(s) 478
HinfI GANTC 8 cut(s) 146, 470, 858, 1473, 1554, 1741, 1817, 2060
HpaII CCGG 5 cut(s) 702, 1648, 1745, 1889, 2103
HphI GGTGA 3 cut(s) 1246, 1597, 2198
Hpy166II GTNNAC 5 cut(s) 97, 533, 1059, 1402, 1900
Hpy188I TCNGA 4 cut(s) 1132, 1639, 2137, 2272
Hpy8I GTNNAC 5 cut(s) 97, 533, 1059, 1402, 1900
HpyCH4III ACNGT 2 cut(s) 1000, 1030
HpyCH4IV ACGT 1 cut(s) 822
HpyF3I CTNAG 7 cut(s) 100, 1287, 1718, 1855, 2051, 2062, 2220
HpySE526I ACGT 1 cut(s) 822
HspAI GCGC 2 cut(s) 1155, 1938
KflI GGGWCCC 1 cut(s) 500
Ksp22I TGATCA 1 cut(s) 1962
LguI GCTCTTC 1 cut(s) 1286
LmnI GCTCC 5 cut(s) 104, 890, 1389, 1507, 2128
LweI GCATC 5 cut(s) 304, 805, 829, 1190, 1706
MaeI CTAG 8 cut(s) 234, 348, 1227, 1527, 1895, 1916, 2012, 2160
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 5 cut(s) 557, 644, 2065, 2083, 2279
MbiI CCGCTC 1 cut(s) 641
MflI RGATCY 4 cut(s) 139, 662, 1245, 1885
MhlI GDGCHC 6 cut(s) 109, 760, 790, 895, 1386, 2297
MlsI TGGCCA 2 cut(s) 466, 657
MluNI TGGCCA 2 cut(s) 466, 657
MlyI GAGTC 5 cut(s) 867, 1482, 1750, 1811, 2054
MmeI TCCRAC 1 cut(s) 2001
Mox20I TGGCCA 2 cut(s) 466, 657
Mph1103I ATGCAT 2 cut(s) 1183, 1699
MroXI GAANNNNTTC 1 cut(s) 2198
MscI TGGCCA 2 cut(s) 466, 657
MslI CAYNNNNRTG 6 cut(s) 114, 243, 300, 407, 956, 1176
Msp20I TGGCCA 2 cut(s) 466, 657
MspA1I CMGCKG 1 cut(s) 971
MspI CCGG 5 cut(s) 702, 1648, 1745, 1889, 2103
MspR9I CCNGG 9 cut(s) 702, 993, 1018, 1208, 1544, 1581, 1649, 1890, 2103
MvaI CCWGG 5 cut(s) 993, 1018, 1208, 1544, 1581
MvnI CGCG 2 cut(s) 762, 1944
NciI CCSGG 4 cut(s) 702, 1649, 1890, 2103
NcoI CCATGG 1 cut(s) 658
NdeI CATATG 1 cut(s) 793
NmuCI GTSAC 3 cut(s) 644, 2065, 2279
NsiI ATGCAT 2 cut(s) 1183, 1699
NspI RCATGY 4 cut(s) 1175, 1400, 1689, 2123
PaeI GCATGC 1 cut(s) 1689
PaeR7I CTCGAG 2 cut(s) 753, 1470
PagI TCATGA 1 cut(s) 1767
PaqCI CACCTGC 1 cut(s) 1304
PciI ACATGT 1 cut(s) 1396
PciSI GCTCTTC 1 cut(s) 1286
PdmI GAANNNNTTC 1 cut(s) 2198
PfeI GAWTC 3 cut(s) 146, 470, 1554
PfoI TCCNGGA 3 cut(s) 700, 1542, 1888
Ple19I CGATCG 2 cut(s) 1047, 1051
PleI GAGTC 5 cut(s) 866, 1481, 1749, 1811, 2054
PpsI GAGTC 5 cut(s) 866, 1481, 1749, 1811, 2054
PpuMI RGGWCCY 1 cut(s) 500
PscI ACATGT 1 cut(s) 1396
PshBI ATTAAT 1 cut(s) 243
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 5 cut(s) 991, 1016, 1206, 1542, 1579
PspFI CCCAGC 1 cut(s) 783
PspGI CCWGG 5 cut(s) 991, 1016, 1206, 1542, 1579
PspPI GGNCC 7 cut(s) 119, 500, 614, 927, 983, 1532, 1619
PspPPI RGGWCCY 1 cut(s) 500
PspXI VCTCGAGB 2 cut(s) 753, 1470
PstI CTGCAG 1 cut(s) 283
PstNI CAGNNNCTG 1 cut(s) 1319
PsuI RGATCY 4 cut(s) 139, 662, 1245, 1885
PvuI CGATCG 2 cut(s) 1047, 1051
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 2 cut(s) 98, 584
RsaNI GTAC 2 cut(s) 97, 583
RseI CAYNNNNRTG 6 cut(s) 114, 243, 300, 407, 956, 1176
SapI GCTCTTC 1 cut(s) 1286
Sau96I GGNCC 7 cut(s) 119, 500, 614, 927, 983, 1532, 1619
SchI GAGTC 5 cut(s) 867, 1482, 1750, 1811, 2054
ScrFI CCNGG 9 cut(s) 702, 993, 1018, 1208, 1544, 1581, 1649, 1890, 2103
SduI GDGCHC 6 cut(s) 109, 760, 790, 895, 1386, 2297
SfaNI GCATC 5 cut(s) 304, 805, 829, 1190, 1706
SfcI CTRYAG 2 cut(s) 279, 1320
Sfr274I CTCGAG 2 cut(s) 753, 1470
SinI GGWCC 2 cut(s) 500, 983
SlaI CTCGAG 2 cut(s) 753, 1470
SmiMI CAYNNNNRTG 6 cut(s) 114, 243, 300, 407, 956, 1176
SmlI CTYRAG 4 cut(s) 187, 753, 1470, 2238
SmoI CTYRAG 4 cut(s) 187, 753, 1470, 2238
SpeI ACTAGT 1 cut(s) 1894
SphI GCATGC 1 cut(s) 1689
SsiI CCGC 4 cut(s) 639, 760, 1188, 1944
SspMI CTAG 8 cut(s) 234, 348, 1227, 1527, 1895, 1916, 2012, 2160
StyD4I CCNGG 9 cut(s) 700, 991, 1016, 1206, 1542, 1579, 1647, 1888, 2101
StyI CCWWGG 2 cut(s) 658, 1226
TaaI ACNGT 2 cut(s) 1000, 1030
TaiI ACGT 1 cut(s) 825
TaqI TCGA 5 cut(s) 144, 754, 861, 1047, 1471
TatI WGTACW 1 cut(s) 96
TfiI GAWTC 3 cut(s) 146, 470, 1554
TscAI CASTG 7 cut(s) 812, 1005, 1414, 1780, 2059, 2070, 2310
TseFI GTSAC 3 cut(s) 644, 2065, 2279
Tsp45I GTSAC 3 cut(s) 644, 2065, 2279
TspDTI ATGAA 7 cut(s) 79, 152, 384, 425, 504, 810, 1512
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 7 cut(s) 812, 1005, 1414, 1780, 2059, 2070, 2310
VpaK11BI GGWCC 2 cut(s) 500, 983
VspI ATTAAT 1 cut(s) 243
XagI CCTNNNNNAGG 1 cut(s) 1547
XapI RAATTY 8 cut(s) 158, 358, 525, 899, 910, 1484, 1632, 2171
XceI RCATGY 4 cut(s) 1175, 1400, 1689, 2123
XcmI CCANNNNNNNNNTGG 1 cut(s) 1453
XhoI CTCGAG 2 cut(s) 753, 1470
XmaJI CCTAGG 1 cut(s) 1226
XmnI GAANNNNTTC 1 cut(s) 2198
XspI CTAG 8 cut(s) 234, 348, 1227, 1527, 1895, 1916, 2012, 2160
Zsp2I ATGCAT 2 cut(s) 1183, 1699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.