Rroxscaffold_1G00006310

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000923
Physical Location & Seq
Forward (+)
8390363 .. 8394046
3684 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00006310.1

Sequence Viewer

Length: 2028 bp
ATGGATCCAAATGTGATATCAGTCTTCCTCAACAAGGGAAGAAAGCTGCAAACTACTCGGTCATGGAATTTTCTTGGATTAGAGAGAAATGGACTGATTCCTTCAGACTCCATTTGGAAGAAAGCAAGGTTTGGCGAAGATACAATAATTGCAAACATAGATACTGGTATTTGGCCAGAATCCAAAAGCTTTAGTGATGAAGGGTTGGGACCTGTCCCTTCCAAGTGGCGTGGAACTTGTCAACATGACACAAAGCAAGTTCATTGCAACAGAAAGCTGATTGGAACTAGGTACTTTAATAATGGTCTTGCCATGTATGCCGGCCCTCTCAACTCGTCGTATTCCACTGTTCGCGACTACGATGGTCATGGATCACATACCCTATCCACGGCTGCGGGTGGGTTTGTTCCAGGAGTTAGTGTTTTTGGCAATGGCAATGGAACCACCAAGGGTGGCTCTCCTCAAGCCCGTGTTGCTGCATATAAGGTATGCTGGCCACCATTTGAAGGTGTTGGGTGTTTCGAGGCAGACATCTTAGCTGCCTTTGATGCTGCAATAAGTGACTGTGTCGATGTAATCTCTGTGTCTCTTGGTGGAGGAACAGAGGAGTTTTTCAACAATGCAATTTCAATAGGGTCCTTCCATGCAGTTAAAAATGGCATTGTTGTGGTTAGTGCAGCTGGAAACTCTGGACCAAATCCAGGGACAGTATTAAACTTGTCGCCATGGTTAGTAACAGTTGGTGCTAGCACCATTGATCGAGAGTTCACAAGCTATGTTGTCCTCGGAAACAAAAAGCAATTGAAGGGAGCAAGCCTTTCAGCTAAAGGCTTACCATTTGAAATGTTTTACCCATTGATCAGTGCTGCAGATGCGAGAGATGCTAATGCATCCACTGGAGAAGCTGAAATTTGCAATGTTGGAGCCCTCGATCCTAGGAAAGTAAAAGGAAAGATTTTGGTGTGCGTTCGAGAATACAACGATAATGAAAGAACCGAGAAGAGCCTGCAGGCTGATATTGCAGGTGCTGTAGGAATGATCTTGGTTAATGATAAGCAAAGTGGAAACGATGTTGTAGCCGATCCTCATGTGCTCCTTGTTTCACATCTCAATTACACCGACGGCAAACATGTCTTTGATTACATTAAATCTACCAAAACTCCTGTGGCTTACCTTACTCCAGTAAAGACTGAATTGGGAACAAAGCCAGCTCCGCTTGTAGCTCCATTTTCATCAAGGGGGCCTAATCTTCTGGAGCAGGCAATCTTTAAGCCTGATATCATTGCACCAGGGGTGAGCATAATCGCTGCTTATACTGAAGCAACAGGGCCAACTTATCAATTATCTGACACTCGCCGGGTTTCTTTTAATGTTCAAACCGGCACTTCAATGGCGTGCCCTCATGTATCCGGAATTGCAGGGCTTCTGAGGAAAATCCATCCAGATTGGAGCCCAGCAGCTATTAAATCTGCAATCATGACAACTGCTACAACACAAGATGACAGCAATGAACCGATGCTCGACTCGTCTTATTTGAAGGCAACACCATTTGCTTATGGTGCAGGACACATTCAACCAAACAACGCAATGGACCCGGGGCTAATTTATAACCTAACAACACTTGATTACTTGAATTTCTTATGTGCTCGAGGATACAATGAAACAATGATCAAATCATTCTCCAATCCACCTTTCAAATGTCCCAAGTCTTTCAGTCTAGGAGACTTCAACTACCCAGCAATTGCAATTCCTAATCTCGGTGCAGAGTTATTGACCGTTACTAGAAGAAAAGTTACAAATGTCGGATCACCTGGCACGTACAAAGTGTGCGTCAAGGCACCACCAGAAGTAATGGTTTTGGTTAAACCTAGAAGCTTGAAATTTGAGAGTATTGGTGAAGTGAAAAAGTTCAAAGTTATATTCAAAGCTAAGGTTAAGGGTAAGCCTCGAGGGTATGCATTTGGAGAGTTGATGTGGTCAGATGGCAATCACCATGTAAAGAGTCCTCTTGCAGTGAAGCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

675

Amino Acids

72.58

Weight (kDa)

8.91

Isoelectric Point (pI)

25.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 45 - 509 5.3e-39 Subtilase family
PA PF02225 299 - 376 5.5e-10 PA domain
fn3_6 PF17766 575 - 672 1.6e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1608
AarI CACCTGC 1 cut(s) 1013
AbsI CCTCGAGG 1 cut(s) 1947
Acc36I ACCTGC 1 cut(s) 1013
AccB1I GGYRCC 1 cut(s) 1837
AccII CGCG 1 cut(s) 354
AccIII TCCGGA 1 cut(s) 1409
AciI CCGC 2 cut(s) 395, 1214
AclWI GGATC 5 cut(s) 12, 379, 926, 1076, 1813
AcoI YGGCCR 2 cut(s) 173, 494
AcsI RAATTY 4 cut(s) 67, 909, 1633, 1880
AcuI CTGAAG 2 cut(s) 87, 1338
AfaI GTAC 2 cut(s) 293, 1820
AfiI CCNNNNNNNGG 5 cut(s) 34, 388, 506, 701, 1757
AflIII ACRYGT 1 cut(s) 1129
AjnI CCWGG 4 cut(s) 409, 700, 1288, 1810
AjuI GAANNNNNNNTTGG 2 cut(s) 1676, 1708
Alw21I GWGCWC 2 cut(s) 1095, 1648
Alw26I GTCTC 2 cut(s) 591, 1716
AlwI GGATC 5 cut(s) 12, 379, 926, 1076, 1813
AlwNI CAGNNNCTG 1 cut(s) 1028
Ama87I CYCGRG 3 cut(s) 1594, 1647, 1947
Aor13HI TCCGGA 1 cut(s) 1409
AoxI GGCC 5 cut(s) 173, 322, 494, 1241, 1328
ApeKI GCWGC 9 cut(s) 46, 392, 476, 539, 551, 677, 866, 1307, 1457
ApoI RAATTY 4 cut(s) 67, 909, 1633, 1880
ArsI GACNNNNNNTTYG 2 cut(s) 1815, 1847
Asp700I GAANNNNTTC 1 cut(s) 1907
AspA2I CCTAGG 1 cut(s) 935
AspS9I GGNCC 7 cut(s) 209, 323, 636, 692, 1241, 1328, 1591
AsuC2I CCSGG 3 cut(s) 1358, 1595, 1596
AsuHPI GGTGA 4 cut(s) 1306, 1800, 1907, 1982
AsuNHI GCTAGC 1 cut(s) 746
AvaI CYCGRG 3 cut(s) 1594, 1647, 1947
AvaII GGWCC 4 cut(s) 209, 636, 692, 1591
AvrII CCTAGG 1 cut(s) 935
BaeGI GKGCMC 1 cut(s) 1400
BalI TGGCCA 2 cut(s) 175, 496
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 1837
BanII GRGCYC 2 cut(s) 928, 1454
BarI GAAGNNNNNNTAC 2 cut(s) 1777, 1809
BbsI GAAGAC 1 cut(s) 16
Bbv12I GWGCWC 2 cut(s) 1095, 1648
BbvI GCAGC 9 cut(s) 33, 379, 463, 526, 538, 689, 853, 1294, 1469
BccI CCATC 3 cut(s) 356, 1446, 1976
BceAI ACGGC 2 cut(s) 405, 1138
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 4 cut(s) 411, 702, 1290, 1812
BciVI GTATCC 2 cut(s) 1417, 1646
BclI TGATCA 2 cut(s) 858, 1668
BcnI CCSGG 3 cut(s) 1358, 1595, 1596
BcoDI GTCTC 2 cut(s) 591, 1716
BfaI CTAG 7 cut(s) 288, 747, 936, 1718, 1782, 1869, 2026
BfmI CTRYAG 3 cut(s) 867, 1007, 1029
BfuAI ACCTGC 1 cut(s) 1013
BfuI GTATCC 2 cut(s) 1417, 1646
BisI GCNGC 9 cut(s) 47, 393, 477, 540, 552, 678, 867, 1308, 1458
BlnI CCTAGG 1 cut(s) 935
BlsI GCNGC 9 cut(s) 48, 394, 478, 541, 553, 679, 868, 1309, 1459
Bme1390I CCNGG 7 cut(s) 411, 702, 1290, 1358, 1595, 1596, 1812
Bme18I GGWCC 4 cut(s) 209, 636, 692, 1591
BmeT110I CYCGRG 3 cut(s) 1594, 1647, 1947
BmgT120I GGNCC 7 cut(s) 209, 323, 636, 692, 1241, 1328, 1591
BmiI GGNNCC 9 cut(s) 6, 210, 442, 637, 925, 1242, 1451, 1593, 1839
BmrFI CCNGG 7 cut(s) 411, 702, 1290, 1358, 1595, 1596, 1812
BmsI GCATC 5 cut(s) 538, 862, 871, 899, 1506
BmtI GCTAGC 1 cut(s) 750
BpiI GAAGAC 1 cut(s) 16
BpmI CTGGAG 3 cut(s) 918, 1164, 1274
Bpu10I CCTNAGC 1 cut(s) 1929
BpuEI CTTGAG 1 cut(s) 447
BpuMI CCSGG 3 cut(s) 1358, 1595, 1596
BsaAI YACGTR 1 cut(s) 1818
BsaBI GATNNNNATC 1 cut(s) 1986
BsaJI CCNNGG 9 cut(s) 387, 447, 701, 725, 784, 935, 1289, 1594, 1595
BsaWI WCCGGW 1 cut(s) 1409
BsaXI ACNNNNNCTCC 4 cut(s) 405, 435, 1144, 1174
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 388, 506, 701, 1757
Bse118I RCCGGY 2 cut(s) 320, 1379
Bse1I ACTGG 3 cut(s) 169, 901, 1181
Bse3DI GCAATG 7 cut(s) 262, 436, 442, 922, 1281, 1513, 1593
Bse8I GATNNNNATC 1 cut(s) 1986
BseAI TCCGGA 1 cut(s) 1409
BseBI CCWGG 4 cut(s) 411, 702, 1290, 1812
BseDI CCNNGG 9 cut(s) 387, 447, 701, 725, 784, 935, 1289, 1594, 1595
BseGI GGATG 2 cut(s) 890, 1438
BseJI GATNNNNATC 1 cut(s) 1986
BseLI CCNNNNNNNGG 5 cut(s) 34, 388, 506, 701, 1757
BseMI GCAATG 7 cut(s) 262, 436, 442, 922, 1281, 1513, 1593
BseMII CTCAG 1 cut(s) 1418
BseNI ACTGG 3 cut(s) 169, 901, 1181
BseRI GAGGAG 2 cut(s) 450, 620
BseSI GKGCMC 1 cut(s) 1400
BseXI GCAGC 9 cut(s) 33, 379, 463, 526, 538, 689, 853, 1294, 1469
BseYI CCCAGC 2 cut(s) 1453, 1735
BsgI GTGCAG 3 cut(s) 696, 1581, 1782
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 5 cut(s) 175, 324, 496, 1243, 1330
BshNI GGYRCC 1 cut(s) 1837
BsiHKAI GWGCWC 2 cut(s) 1095, 1648
BsiHKCI CYCGRG 3 cut(s) 1594, 1647, 1947
BsiSI CCGG 5 cut(s) 321, 1357, 1380, 1410, 1595
BslFI GGGAC 4 cut(s) 200, 222, 718, 1686
BslI CCNNNNNNNGG 5 cut(s) 34, 388, 506, 701, 1757
BsmAI GTCTC 2 cut(s) 591, 1716
BsmFI GGGAC 4 cut(s) 200, 222, 718, 1686
BsnI GGCC 5 cut(s) 175, 324, 496, 1243, 1330
BsoBI CYCGRG 3 cut(s) 1594, 1647, 1947
Bsp1286I GDGCHC 5 cut(s) 928, 1095, 1400, 1454, 1648
Bsp13I TCCGGA 1 cut(s) 1409
Bsp143I GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
Bsp19I CCATGG 1 cut(s) 725
Bsp68I TCGCGA 1 cut(s) 354
BspACI CCGC 2 cut(s) 395, 1214
BspANI GGCC 5 cut(s) 175, 324, 496, 1243, 1330
BspCNI CTCAG 1 cut(s) 1419
BspEI TCCGGA 1 cut(s) 1409
BspFNI CGCG 1 cut(s) 354
BspHI TCATGA 1 cut(s) 1476
BspLI GGNNCC 9 cut(s) 6, 210, 442, 637, 925, 1242, 1451, 1593, 1839
BspMAI CTGCAG 2 cut(s) 871, 1011
BspMI ACCTGC 1 cut(s) 1013
BspOI GCTAGC 1 cut(s) 750
BspPI GGATC 5 cut(s) 12, 379, 926, 1076, 1813
BspQI GCTCTTC 1 cut(s) 995
BspT107I GGYRCC 1 cut(s) 1837
BsrDI GCAATG 7 cut(s) 262, 436, 442, 922, 1281, 1513, 1593
BsrFI RCCGGY 2 cut(s) 320, 1379
BsrI ACTGG 3 cut(s) 169, 901, 1181
BssAI RCCGGY 2 cut(s) 320, 1379
BssECI CCNNGG 9 cut(s) 387, 447, 701, 725, 784, 935, 1289, 1594, 1595
BssMI GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
BssT1I CCWWGG 3 cut(s) 447, 725, 935
Bst2UI CCWGG 4 cut(s) 411, 702, 1290, 1812
Bst4CI ACNGT 5 cut(s) 349, 566, 709, 739, 1777
Bst6I CTCTTC 1 cut(s) 995
BstBAI YACGTR 1 cut(s) 1818
BstC8I GCNNGC 9 cut(s) 322, 494, 748, 814, 1007, 1011, 1209, 1260, 1396
BstDEI CTNAG 3 cut(s) 535, 1427, 1929
BstDSI CCRYGG 2 cut(s) 387, 725
BstENI CCTNNNNNAGG 1 cut(s) 32
BstF5I GGATG 2 cut(s) 890, 1438
BstFNI CGCG 1 cut(s) 354
BstKTI GATC 9 cut(s) 7, 374, 760, 861, 934, 1041, 1084, 1671, 1808
BstMAI GTCTC 2 cut(s) 591, 1716
BstMBI GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
BstMWI GCNNNNNNNGC 8 cut(s) 317, 473, 548, 872, 881, 1019, 1213, 1559
BstNI CCWGG 4 cut(s) 411, 702, 1290, 1812
BstNSI RCATGY 1 cut(s) 1133
BstSCI CCNGG 7 cut(s) 409, 700, 1288, 1356, 1593, 1594, 1810
BstSFI CTRYAG 3 cut(s) 867, 1007, 1029
BstSLI GKGCMC 1 cut(s) 1400
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 9 cut(s) 33, 379, 463, 526, 538, 689, 853, 1294, 1469
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 2 cut(s) 1417, 1646
BsuRI GGCC 5 cut(s) 175, 324, 496, 1243, 1330
BtgI CCRYGG 2 cut(s) 387, 725
BtsCI GGATG 2 cut(s) 890, 1438
BtsI GCAGTG 1 cut(s) 2019
BtsIMutI CAGTG 4 cut(s) 345, 868, 894, 2019
BtuMI TCGCGA 1 cut(s) 354
BveI ACCTGC 1 cut(s) 1013
Cac8I GCNNGC 9 cut(s) 322, 494, 748, 814, 1007, 1011, 1209, 1260, 1396
CaiI CAGNNNCTG 1 cut(s) 1028
CciI TCATGA 1 cut(s) 1476
Cfr10I RCCGGY 2 cut(s) 320, 1379
Cfr13I GGNCC 7 cut(s) 209, 323, 636, 692, 1241, 1328, 1591
Cfr9I CCCGGG 1 cut(s) 1594
CseI GACGC 1 cut(s) 1819
Csp6I GTAC 2 cut(s) 292, 1819
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviQI GTAC 2 cut(s) 292, 1819
DdeI CTNAG 3 cut(s) 535, 1427, 1929
DpnI GATC 9 cut(s) 6, 373, 759, 860, 933, 1040, 1083, 1670, 1807
DpnII GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
EaeI YGGCCR 2 cut(s) 173, 494
Eam1104I CTCTTC 1 cut(s) 995
EarI CTCTTC 1 cut(s) 995
Eco130I CCWWGG 3 cut(s) 447, 725, 935
Eco24I GRGCYC 2 cut(s) 928, 1454
Eco32I GATATC 2 cut(s) 18, 1279
Eco47I GGWCC 4 cut(s) 209, 636, 692, 1591
Eco57I CTGAAG 2 cut(s) 87, 1338
Eco88I CYCGRG 3 cut(s) 1594, 1647, 1947
EcoNI CCTNNNNNAGG 1 cut(s) 32
EcoO109I RGGNCCY 3 cut(s) 209, 636, 1241
EcoRII CCWGG 4 cut(s) 409, 700, 1288, 1810
EcoRV GATATC 2 cut(s) 18, 1279
EcoT14I CCWWGG 3 cut(s) 447, 725, 935
EcoT22I ATGCAT 2 cut(s) 892, 1960
EcoT38I GRGCYC 2 cut(s) 928, 1454
ErhI CCWWGG 3 cut(s) 447, 725, 935
FaqI GGGAC 4 cut(s) 200, 222, 718, 1686
FauI CCCGC 1 cut(s) 388
FbaI TGATCA 2 cut(s) 858, 1668
Fnu4HI GCNGC 9 cut(s) 47, 393, 477, 540, 552, 678, 867, 1308, 1458
FokI GGATG 2 cut(s) 877, 1425
FriOI GRGCYC 2 cut(s) 928, 1454
Fsp4HI GCNGC 9 cut(s) 47, 393, 477, 540, 552, 678, 867, 1308, 1458
FspBI CTAG 7 cut(s) 288, 747, 936, 1718, 1782, 1869, 2026
GluI GCNGC 9 cut(s) 47, 393, 477, 540, 552, 678, 867, 1308, 1458
GsaI CCCAGC 2 cut(s) 1457, 1739
GsuI CTGGAG 3 cut(s) 918, 1164, 1274
HaeIII GGCC 5 cut(s) 175, 324, 496, 1243, 1330
HapII CCGG 5 cut(s) 321, 1357, 1380, 1410, 1595
HgaI GACGC 1 cut(s) 1819
HincII GTYRAC 1 cut(s) 242
HindII GTYRAC 1 cut(s) 242
HindIII AAGCTT 2 cut(s) 187, 1873
HinfI GANTC 5 cut(s) 97, 107, 179, 1523, 2002
HpaII CCGG 5 cut(s) 321, 1357, 1380, 1410, 1595
HphI GGTGA 4 cut(s) 1306, 1800, 1907, 1982
Hpy166II GTNNAC 2 cut(s) 242, 768
Hpy188I TCNGA 6 cut(s) 106, 788, 1348, 1428, 1805, 1981
Hpy188III TCNNGA 8 cut(s) 353, 690, 761, 971, 1253, 1410, 1442, 1477
Hpy8I GTNNAC 2 cut(s) 242, 768
Hpy99I CGWCG 2 cut(s) 340, 1124
HpyAV CCTTC 7 cut(s) 111, 194, 228, 500, 649, 799, 1531
HpyCH4III ACNGT 5 cut(s) 349, 566, 709, 739, 1777
HpyCH4IV ACGT 1 cut(s) 1817
HpyF10VI GCNNNNNNNGC 8 cut(s) 317, 473, 548, 872, 881, 1019, 1213, 1559
HpyF3I CTNAG 3 cut(s) 535, 1427, 1929
HpySE526I ACGT 1 cut(s) 1817
Kpn2I TCCGGA 1 cut(s) 1409
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Ksp22I TGATCA 2 cut(s) 858, 1668
Kzo9I GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
LguI GCTCTTC 1 cut(s) 995
LmnI GCTCC 7 cut(s) 809, 923, 1098, 1216, 1228, 1255, 1449
Lsp1109I GCAGC 9 cut(s) 33, 379, 463, 526, 538, 689, 853, 1294, 1469
LweI GCATC 5 cut(s) 538, 862, 871, 899, 1506
MaeI CTAG 7 cut(s) 288, 747, 936, 1718, 1782, 1869, 2026
MaeII ACGT 1 cut(s) 1817
MaeIII GTNAC 4 cut(s) 560, 733, 1777, 1792
MalI GATC 9 cut(s) 6, 373, 759, 860, 933, 1040, 1083, 1670, 1807
MboI GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
MboII GAAGA 7 cut(s) 16, 51, 130, 149, 1012, 1241, 1797
MfeI CAATTG 2 cut(s) 800, 1740
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 5 cut(s) 928, 1095, 1400, 1454, 1648
MlsI TGGCCA 2 cut(s) 175, 496
MluNI TGGCCA 2 cut(s) 175, 496
MlyI GAGTC 3 cut(s) 101, 1517, 2011
MmeI TCCRAC 2 cut(s) 901, 1783
Mox20I TGGCCA 2 cut(s) 175, 496
Mph1103I ATGCAT 2 cut(s) 892, 1960
MroI TCCGGA 1 cut(s) 1409
MroNI GCCGGC 1 cut(s) 320
MroXI GAANNNNTTC 1 cut(s) 1907
MscI TGGCCA 2 cut(s) 175, 496
MslI CAYNNNNRTG 2 cut(s) 665, 1388
Msp20I TGGCCA 2 cut(s) 175, 496
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 5 cut(s) 321, 1357, 1380, 1410, 1595
MspR9I CCNGG 7 cut(s) 411, 702, 1290, 1358, 1595, 1596, 1812
MunI CAATTG 2 cut(s) 800, 1740
MvaI CCWGG 4 cut(s) 411, 702, 1290, 1812
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 8 cut(s) 317, 473, 548, 872, 881, 1019, 1213, 1559
NaeI GCCGGC 1 cut(s) 322
NciI CCSGG 3 cut(s) 1358, 1595, 1596
NcoI CCATGG 1 cut(s) 725
NdeII GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
NgoMIV GCCGGC 1 cut(s) 320
NheI GCTAGC 1 cut(s) 746
NlaIV GGNNCC 9 cut(s) 6, 210, 442, 637, 925, 1242, 1451, 1593, 1839
NmuCI GTSAC 1 cut(s) 560
NruI TCGCGA 1 cut(s) 354
NsiI ATGCAT 2 cut(s) 892, 1960
NspI RCATGY 1 cut(s) 1133
PaeR7I CTCGAG 2 cut(s) 1647, 1947
PagI TCATGA 1 cut(s) 1476
PaqCI CACCTGC 1 cut(s) 1013
PciI ACATGT 1 cut(s) 1129
PciSI GCTCTTC 1 cut(s) 995
PdiI GCCGGC 1 cut(s) 322
PdmI GAANNNNTTC 1 cut(s) 1907
PfeI GAWTC 2 cut(s) 97, 179
PflFI GACNNNGTC 1 cut(s) 566
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 9 cut(s) 48, 394, 478, 541, 553, 679, 868, 1309, 1459
PleI GAGTC 3 cut(s) 101, 1517, 2010
PpsI GAGTC 3 cut(s) 101, 1517, 2010
Ppu21I YACGTR 1 cut(s) 1818
PpuMI RGGWCCY 2 cut(s) 209, 636
PscI ACATGT 1 cut(s) 1129
PsiI TTATAA 1 cut(s) 1608
Psp5II RGGWCCY 2 cut(s) 209, 636
Psp6I CCWGG 4 cut(s) 409, 700, 1288, 1810
PspFI CCCAGC 2 cut(s) 1453, 1735
PspGI CCWGG 4 cut(s) 409, 700, 1288, 1810
PspN4I GGNNCC 9 cut(s) 6, 210, 442, 637, 925, 1242, 1451, 1593, 1839
PspPI GGNCC 7 cut(s) 209, 323, 636, 692, 1241, 1328, 1591
PspPPI RGGWCCY 2 cut(s) 209, 636
PspXI VCTCGAGB 2 cut(s) 1647, 1947
PstI CTGCAG 2 cut(s) 871, 1011
PstNI CAGNNNCTG 1 cut(s) 1028
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 566
PvuII CAGCTG 1 cut(s) 680
RruI TCGCGA 1 cut(s) 354
RsaI GTAC 2 cut(s) 293, 1820
RsaNI GTAC 2 cut(s) 292, 1819
RseI CAYNNNNRTG 2 cut(s) 665, 1388
SapI GCTCTTC 1 cut(s) 995
SatI GCNGC 9 cut(s) 47, 393, 477, 540, 552, 678, 867, 1308, 1458
Sau3AI GATC 9 cut(s) 4, 371, 757, 858, 931, 1038, 1081, 1668, 1805
Sau96I GGNCC 7 cut(s) 209, 323, 636, 692, 1241, 1328, 1591
SbfI CCTGCAGG 1 cut(s) 1011
SchI GAGTC 3 cut(s) 101, 1517, 2011
ScrFI CCNGG 7 cut(s) 411, 702, 1290, 1358, 1595, 1596, 1812
SdaI CCTGCAGG 1 cut(s) 1011
SduI GDGCHC 5 cut(s) 928, 1095, 1400, 1454, 1648
SfaNI GCATC 5 cut(s) 538, 862, 871, 899, 1506
SfcI CTRYAG 3 cut(s) 867, 1007, 1029
Sfr274I CTCGAG 2 cut(s) 1647, 1947
SinI GGWCC 4 cut(s) 209, 636, 692, 1591
SlaI CTCGAG 2 cut(s) 1647, 1947
SmaI CCCGGG 1 cut(s) 1596
SmiMI CAYNNNNRTG 2 cut(s) 665, 1388
SmlI CTYRAG 3 cut(s) 462, 1647, 1947
SmoI CTYRAG 3 cut(s) 462, 1647, 1947
Sse8387I CCTGCAGG 1 cut(s) 1011
SsiI CCGC 2 cut(s) 395, 1214
SspMI CTAG 7 cut(s) 288, 747, 936, 1718, 1782, 1869, 2026
StyD4I CCNGG 7 cut(s) 409, 700, 1288, 1356, 1593, 1594, 1810
StyI CCWWGG 3 cut(s) 447, 725, 935
TaaI ACNGT 5 cut(s) 349, 566, 709, 739, 1777
TaiI ACGT 1 cut(s) 1820
TaqI TCGA 8 cut(s) 522, 570, 760, 930, 970, 1521, 1648, 1948
TaqII GACCGA 1 cut(s) 48
TfiI GAWTC 2 cut(s) 97, 179
TscAI CASTG 4 cut(s) 352, 868, 901, 2019
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 9 cut(s) 46, 392, 476, 539, 551, 677, 866, 1307, 1457
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 6 cut(s) 213, 251, 1002, 1221, 1524, 1674
TspMI CCCGGG 1 cut(s) 1594
TspRI CASTG 4 cut(s) 352, 868, 901, 2019
Tth111I GACNNNGTC 1 cut(s) 566
VpaK11BI GGWCC 4 cut(s) 209, 636, 692, 1591
XagI CCTNNNNNAGG 1 cut(s) 32
XapI RAATTY 4 cut(s) 67, 909, 1633, 1880
XceI RCATGY 1 cut(s) 1133
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XhoI CTCGAG 2 cut(s) 1647, 1947
XmaI CCCGGG 1 cut(s) 1594
XmaJI CCTAGG 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 1907
XspI CTAG 7 cut(s) 288, 747, 936, 1718, 1782, 1869, 2026
Zsp2I ATGCAT 2 cut(s) 892, 1960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.