Rh5DG528800

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000923
Physical Location & Seq
Reverse (-)
81593488 .. 81598096
4609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG528800.1

Sequence Viewer

Length: 2082 bp
ATGGTTTTGCTGCAATTCTTGAAGAGGAAGAAGCTGCTCAGATTGCTAGATGCAGAGGATCCAAATGTGATATCAGTCTTCCTAAACAAGGGAAGAAAGCTGCAAACTACCCGGTCATGGAATTTTCTTGGATTAGAGAGAAATGGACTGATTCCTTCAGACTCCATTTGGAAGAAAGCAAGGTTTGGCGAAGATACAATAATTGCAAACATAGATACTGGTGTTTGGCCAGAATCCAAAAGCTTAAGTGATGAAGGGTTGGGACCTGTCCCTTCCAAGTGGCGTGGAACTTGTCAACATGATACAAAGCAAGTTCATTGCAACAGAAAGCTGATTGGAACTAGGTACTTTAATAATGGTCTTGCCATGTATGCCGGCCCTCTCAACTCGTCGTATTCCACTGTTCGCGACTACGATGGTCATGGATCACATACCCTATCCACGGCTGCGGGTGGCTTTGTTCCAGGAGTTAGTGTTTTTGGCAATGGCAATGGAACCACCAAGGGTGGCTCTCCTCAAGCCCGTGTTGCTGCATATAAGGTATGCTGGCCACCATTTGAAGGTGTTGGGTGCTTCGAGGCAGACATCTTAGCTGCCTTTGATGCTGCAATAAGTGACGGTGTCGATGTAATCTCTGTGTCTCTTGGTGGAGGAACAGAGGAGTTTTTCAACAATGCAATTTCAATAGGGTCCTTCCATGCAGTTAAAAAGGGCATTGTTGTGGTTAGTGCAGCTGGAAACTCTGGACCAAATCCAGGGACAGTATTAAACTTGTCGCCATGGTTAGTAACAGTTGGTGCTAGCACCATTGATCGAGAGTTCACAAGCTATGTTGTCCTCGGAAACAAAAAGCAATTGAAGGGAGCAAGCCTTTCAGCTAAAGGCTTACCATCTGAAATGTTTTACGCGTTGATCAGTGCTGCAGATGCGAGAGATGCTGATGCATCCACTGGAGAAGCTGAAATTTGCAATGTTGGAGCCCTCGATCCTAGGAAAGTAAAAGGAAAGATTTTGGTGTGCGTTCGAGAATACAACGATAATGAAAGAACCGAGAAGAGCCTGCAGGCTGATATTGCAGGTGCTGTAGGAATGATCTTGGTTAATGATAAGCAAAGTGGAAACGATGTTGTAGCCGATCCTCATGTGCTCCTTGTTTCACATCTCAATTACACCGACGGCAAACATGTCTTTGATTACATTAAATCTACCAAAACTCCTGTGGCTTACCTTACTCCAGTAAAGACTGAATTGGGAACAAAGCCAGCTCCGCTTGTAGCTCCATTTTCATCAAGGGGGCCTAATCTTCTGGAGCAGGCAATCTTTAAGCCTGATATCATTGCACCAGGGGTGAGTATAATTGCTGCTTATACTGAAGCAACAGGGCCAACTTATCAATTATCTGATACTCGCCGGGTTTCTTTTAATGTTCAAACCGGCACTTCAATGGCGTGCCCTCATGTATCCGGAATTGCAGGGCTTCTGAGGAAACTCCATCCAGATTGGAGCCCAGCAGCTATTAAATCTGCAATCATGACAACTGCTACAACACAAGATGACAGCAATGAACCGATGCTTGACTCGTCTTATTTGAAGGCAACACCATTTGCTTATGGTGCAGGACACATTCAACCAAACAACGCAATGGACCCGGGGCTAATTTATAACCTAACAACACTTGATTACTTGAATTTCTTATGTGCTCGAGGATACAATGAAACAATGATCAAATCATTCTCCAATCCACCTTTCAAATGTCCCAAGTCTTTCAGTCTAGGAGACTTCAACTACCCAGCAATTGCAATTCCTAATCTCGGTGCCGAGTTAGTGACCGTTACTAGAAGAAAAGTTACAAATGTCGGATCACCTGGCACGTACAAAGTGTGCGTCAAGGCACCACCAGAAGTAATGGTCTTGGTTAAACCTAGAAGCTTGAAATTTGAGAGTATTGGTGAAGTGAAAAAGTTCAAAGTTATATTCAAAGCCAAGGTTAAGGGTAAGCCACGAGGGTATGCATTTGGAGAGTTGATGTGGTCAGATGGCAATCACCATGTAAAGAGTCCTCTTGCAGTGAAGCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

693

Amino Acids

74.58

Weight (kDa)

9.07

Isoelectric Point (pI)

26.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 63 - 527 1.3e-40 Subtilase family
PA PF02225 321 - 394 1.2e-09 PA domain
fn3_6 PF17766 593 - 690 7.5e-25 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1662
AarI CACCTGC 1 cut(s) 1067
Acc36I ACCTGC 1 cut(s) 1067
AccB1I GGYRCC 2 cut(s) 1814, 1891
AccII CGCG 2 cut(s) 408, 908
AccIII TCCGGA 1 cut(s) 1463
AciI CCGC 2 cut(s) 449, 1268
AclWI GGATC 6 cut(s) 53, 66, 433, 980, 1130, 1867
AcoI YGGCCR 2 cut(s) 227, 548
AcsI RAATTY 4 cut(s) 121, 963, 1687, 1934
AcuI CTGAAG 2 cut(s) 141, 1392
AfaI GTAC 2 cut(s) 347, 1874
AfiI CCNNNNNNNGG 6 cut(s) 88, 117, 442, 560, 755, 1811
AflII CTTAAG 1 cut(s) 244
AflIII ACRYGT 2 cut(s) 906, 1183
AjnI CCWGG 4 cut(s) 463, 754, 1342, 1864
AjuI GAANNNNNNNTTGG 2 cut(s) 1730, 1762
Alw21I GWGCWC 2 cut(s) 1149, 1702
Alw26I GTCTC 2 cut(s) 645, 1770
AlwI GGATC 6 cut(s) 53, 66, 433, 980, 1130, 1867
AlwNI CAGNNNCTG 1 cut(s) 1082
Ama87I CYCGRG 2 cut(s) 1648, 1701
Aor13HI TCCGGA 1 cut(s) 1463
AoxI GGCC 5 cut(s) 227, 376, 548, 1295, 1382
ApoI RAATTY 4 cut(s) 121, 963, 1687, 1934
ArsI GACNNNNNNTTYG 2 cut(s) 1869, 1901
Asp700I GAANNNNTTC 1 cut(s) 1961
AspA2I CCTAGG 1 cut(s) 989
AspS9I GGNCC 7 cut(s) 263, 377, 690, 746, 1295, 1382, 1645
AsuC2I CCSGG 4 cut(s) 112, 1412, 1649, 1650
AsuHPI GGTGA 4 cut(s) 1360, 1854, 1961, 2036
AsuNHI GCTAGC 1 cut(s) 800
AvaI CYCGRG 2 cut(s) 1648, 1701
AvaII GGWCC 4 cut(s) 263, 690, 746, 1645
AvrII CCTAGG 1 cut(s) 989
BaeGI GKGCMC 1 cut(s) 1454
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
BalI TGGCCA 2 cut(s) 229, 550
BamHI GGATCC 1 cut(s) 58
BanI GGYRCC 2 cut(s) 1814, 1891
BanII GRGCYC 2 cut(s) 982, 1508
BarI GAAGNNNNNNTAC 2 cut(s) 1831, 1863
BauI CACGAG 1 cut(s) 2001
BbsI GAAGAC 1 cut(s) 70
Bbv12I GWGCWC 2 cut(s) 1149, 1702
BccI CCATC 4 cut(s) 410, 898, 1500, 2030
BceAI ACGGC 2 cut(s) 459, 1192
BciT130I CCWGG 4 cut(s) 465, 756, 1344, 1866
BciVI GTATCC 2 cut(s) 1471, 1700
BclI TGATCA 2 cut(s) 912, 1722
BcnI CCSGG 4 cut(s) 112, 1412, 1649, 1650
BcoDI GTCTC 2 cut(s) 645, 1770
BfaI CTAG 8 cut(s) 47, 342, 801, 990, 1772, 1836, 1923, 2080
BfmI CTRYAG 3 cut(s) 921, 1061, 1083
BfrI CTTAAG 1 cut(s) 244
BfuAI ACCTGC 1 cut(s) 1067
BfuI GTATCC 2 cut(s) 1471, 1700
BlnI CCTAGG 1 cut(s) 989
Bme1390I CCNGG 8 cut(s) 112, 465, 756, 1344, 1412, 1649, 1650, 1866
Bme18I GGWCC 4 cut(s) 263, 690, 746, 1645
BmeT110I CYCGRG 2 cut(s) 1648, 1701
BmgT120I GGNCC 7 cut(s) 263, 377, 690, 746, 1295, 1382, 1645
BmrFI CCNGG 8 cut(s) 112, 465, 756, 1344, 1412, 1649, 1650, 1866
BmsI GCATC 7 cut(s) 40, 592, 916, 925, 931, 953, 1560
BmtI GCTAGC 1 cut(s) 804
BpiI GAAGAC 1 cut(s) 70
BpmI CTGGAG 3 cut(s) 972, 1218, 1328
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 4 cut(s) 112, 1412, 1649, 1650
BsaAI YACGTR 1 cut(s) 1872
BsaBI GATNNNNATC 1 cut(s) 2040
BsaWI WCCGGW 1 cut(s) 1463
BsaXI ACNNNNNCTCC 4 cut(s) 459, 489, 1198, 1228
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 117, 442, 560, 755, 1811
Bse118I RCCGGY 2 cut(s) 374, 1433
Bse1I ACTGG 3 cut(s) 223, 955, 1235
Bse3DI GCAATG 7 cut(s) 316, 490, 496, 976, 1335, 1567, 1647
Bse8I GATNNNNATC 1 cut(s) 2040
BseAI TCCGGA 1 cut(s) 1463
BseBI CCWGG 4 cut(s) 465, 756, 1344, 1866
BseGI GGATG 2 cut(s) 944, 1492
BseJI GATNNNNATC 1 cut(s) 2040
BseLI CCNNNNNNNGG 6 cut(s) 88, 117, 442, 560, 755, 1811
BseMI GCAATG 7 cut(s) 316, 490, 496, 976, 1335, 1567, 1647
BseMII CTCAG 2 cut(s) 52, 1472
BseNI ACTGG 3 cut(s) 223, 955, 1235
BseRI GAGGAG 2 cut(s) 504, 674
BseSI GKGCMC 1 cut(s) 1454
BseYI CCCAGC 2 cut(s) 1507, 1789
BsgI GTGCAG 2 cut(s) 750, 1635
Bsh1236I CGCG 2 cut(s) 408, 908
BshFI GGCC 5 cut(s) 229, 378, 550, 1297, 1384
BshNI GGYRCC 2 cut(s) 1814, 1891
BsiHKAI GWGCWC 2 cut(s) 1149, 1702
BsiHKCI CYCGRG 2 cut(s) 1648, 1701
BsiSI CCGG 6 cut(s) 112, 375, 1411, 1434, 1464, 1649
BslFI GGGAC 4 cut(s) 254, 276, 772, 1740
BslI CCNNNNNNNGG 6 cut(s) 88, 117, 442, 560, 755, 1811
BsmAI GTCTC 2 cut(s) 645, 1770
BsmFI GGGAC 4 cut(s) 254, 276, 772, 1740
BsnI GGCC 5 cut(s) 229, 378, 550, 1297, 1384
BsoBI CYCGRG 2 cut(s) 1648, 1701
Bsp1286I GDGCHC 5 cut(s) 982, 1149, 1454, 1508, 1702
Bsp13I TCCGGA 1 cut(s) 1463
Bsp143I GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
Bsp19I CCATGG 1 cut(s) 779
Bsp68I TCGCGA 1 cut(s) 408
BspACI CCGC 2 cut(s) 449, 1268
BspANI GGCC 5 cut(s) 229, 378, 550, 1297, 1384
BspCNI CTCAG 2 cut(s) 51, 1473
BspEI TCCGGA 1 cut(s) 1463
BspFNI CGCG 2 cut(s) 408, 908
BspHI TCATGA 1 cut(s) 1530
BspMAI CTGCAG 2 cut(s) 925, 1065
BspMI ACCTGC 1 cut(s) 1067
BspOI GCTAGC 1 cut(s) 804
BspPI GGATC 6 cut(s) 53, 66, 433, 980, 1130, 1867
BspQI GCTCTTC 1 cut(s) 1049
BspT107I GGYRCC 2 cut(s) 1814, 1891
BspTI CTTAAG 1 cut(s) 244
BsrDI GCAATG 7 cut(s) 316, 490, 496, 976, 1335, 1567, 1647
BsrFI RCCGGY 2 cut(s) 374, 1433
BsrI ACTGG 3 cut(s) 223, 955, 1235
BssAI RCCGGY 2 cut(s) 374, 1433
BssMI GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
BssSI CACGAG 1 cut(s) 2001
BssT1I CCWWGG 4 cut(s) 501, 779, 989, 1983
Bst2BI CACGAG 1 cut(s) 2001
Bst2UI CCWGG 4 cut(s) 465, 756, 1344, 1866
Bst4CI ACNGT 5 cut(s) 403, 620, 763, 793, 1831
Bst6I CTCTTC 2 cut(s) 17, 1049
BstAFI CTTAAG 1 cut(s) 244
BstBAI YACGTR 1 cut(s) 1872
BstC8I GCNNGC 9 cut(s) 376, 548, 802, 868, 1061, 1065, 1263, 1314, 1450
BstDEI CTNAG 3 cut(s) 38, 589, 1481
BstDSI CCRYGG 2 cut(s) 441, 779
BstENI CCTNNNNNAGG 1 cut(s) 86
BstF5I GGATG 2 cut(s) 944, 1492
BstFNI CGCG 2 cut(s) 408, 908
BstKTI GATC 9 cut(s) 61, 428, 814, 915, 988, 1095, 1138, 1725, 1862
BstMAI GTCTC 2 cut(s) 645, 1770
BstMBI GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
BstMWI GCNNNNNNNGC 9 cut(s) 43, 371, 527, 602, 926, 935, 1073, 1267, 1613
BstNI CCWGG 4 cut(s) 465, 756, 1344, 1866
BstNSI RCATGY 1 cut(s) 1187
BstSCI CCNGG 8 cut(s) 110, 463, 754, 1342, 1410, 1647, 1648, 1864
BstSFI CTRYAG 3 cut(s) 921, 1061, 1083
BstSLI GKGCMC 1 cut(s) 1454
BstUI CGCG 2 cut(s) 408, 908
BstV2I GAAGAC 1 cut(s) 70
BstX2I RGATCY 1 cut(s) 58
BstYI RGATCY 1 cut(s) 58
BsuI GTATCC 2 cut(s) 1471, 1700
BsuRI GGCC 5 cut(s) 229, 378, 550, 1297, 1384
BtgI CCRYGG 2 cut(s) 441, 779
BtsCI GGATG 2 cut(s) 944, 1492
BtsI GCAGTG 1 cut(s) 2073
BtsIMutI CAGTG 4 cut(s) 399, 922, 948, 2073
BtuMI TCGCGA 1 cut(s) 408
BveI ACCTGC 1 cut(s) 1067
Cac8I GCNNGC 9 cut(s) 376, 548, 802, 868, 1061, 1065, 1263, 1314, 1450
CaiI CAGNNNCTG 1 cut(s) 1082
CciI TCATGA 1 cut(s) 1530
Cfr10I RCCGGY 2 cut(s) 374, 1433
Cfr13I GGNCC 7 cut(s) 263, 377, 690, 746, 1295, 1382, 1645
Cfr9I CCCGGG 1 cut(s) 1648
CseI GACGC 1 cut(s) 1873
Csp6I GTAC 2 cut(s) 346, 1873
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviQI GTAC 2 cut(s) 346, 1873
DdeI CTNAG 3 cut(s) 38, 589, 1481
DpnI GATC 9 cut(s) 60, 427, 813, 914, 987, 1094, 1137, 1724, 1861
DpnII GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
EaeI YGGCCR 2 cut(s) 227, 548
Eam1104I CTCTTC 2 cut(s) 17, 1049
EarI CTCTTC 2 cut(s) 17, 1049
Eco130I CCWWGG 4 cut(s) 501, 779, 989, 1983
Eco24I GRGCYC 2 cut(s) 982, 1508
Eco32I GATATC 2 cut(s) 72, 1333
Eco47I GGWCC 4 cut(s) 263, 690, 746, 1645
Eco57I CTGAAG 2 cut(s) 141, 1392
Eco88I CYCGRG 2 cut(s) 1648, 1701
EcoNI CCTNNNNNAGG 1 cut(s) 86
EcoO109I RGGNCCY 3 cut(s) 263, 690, 1295
EcoRII CCWGG 4 cut(s) 463, 754, 1342, 1864
EcoRV GATATC 2 cut(s) 72, 1333
EcoT14I CCWWGG 4 cut(s) 501, 779, 989, 1983
EcoT22I ATGCAT 2 cut(s) 946, 2014
EcoT38I GRGCYC 2 cut(s) 982, 1508
ErhI CCWWGG 4 cut(s) 501, 779, 989, 1983
FaqI GGGAC 4 cut(s) 254, 276, 772, 1740
FauI CCCGC 1 cut(s) 442
FbaI TGATCA 2 cut(s) 912, 1722
FokI GGATG 2 cut(s) 931, 1479
FriOI GRGCYC 2 cut(s) 982, 1508
FspBI CTAG 8 cut(s) 47, 342, 801, 990, 1772, 1836, 1923, 2080
GsaI CCCAGC 2 cut(s) 1511, 1793
GsuI CTGGAG 3 cut(s) 972, 1218, 1328
HaeIII GGCC 5 cut(s) 229, 378, 550, 1297, 1384
HapII CCGG 6 cut(s) 112, 375, 1411, 1434, 1464, 1649
HgaI GACGC 1 cut(s) 1873
HincII GTYRAC 1 cut(s) 296
HindII GTYRAC 1 cut(s) 296
HindIII AAGCTT 2 cut(s) 241, 1927
HinfI GANTC 5 cut(s) 151, 161, 233, 1577, 2056
HpaII CCGG 6 cut(s) 112, 375, 1411, 1434, 1464, 1649
HphI GGTGA 4 cut(s) 1360, 1854, 1961, 2036
Hpy166II GTNNAC 2 cut(s) 296, 822
Hpy188I TCNGA 8 cut(s) 41, 160, 842, 895, 1402, 1482, 1859, 2035
Hpy188III TCNNGA 9 cut(s) 19, 407, 744, 815, 1025, 1307, 1464, 1496, 1531
Hpy8I GTNNAC 2 cut(s) 296, 822
Hpy99I CGWCG 2 cut(s) 394, 1178
HpyAV CCTTC 7 cut(s) 165, 248, 282, 554, 703, 853, 1585
HpyCH4III ACNGT 5 cut(s) 403, 620, 763, 793, 1831
HpyCH4IV ACGT 1 cut(s) 1871
HpyF10VI GCNNNNNNNGC 9 cut(s) 43, 371, 527, 602, 926, 935, 1073, 1267, 1613
HpyF3I CTNAG 3 cut(s) 38, 589, 1481
HpySE526I ACGT 1 cut(s) 1871
Kpn2I TCCGGA 1 cut(s) 1463
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Ksp22I TGATCA 2 cut(s) 912, 1722
Kzo9I GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
LguI GCTCTTC 1 cut(s) 1049
LmnI GCTCC 7 cut(s) 863, 977, 1152, 1270, 1282, 1309, 1503
LweI GCATC 7 cut(s) 40, 592, 916, 925, 931, 953, 1560
MaeI CTAG 8 cut(s) 47, 342, 801, 990, 1772, 1836, 1923, 2080
MaeII ACGT 1 cut(s) 1871
MaeIII GTNAC 5 cut(s) 614, 787, 1825, 1831, 1846
MalI GATC 9 cut(s) 60, 427, 813, 914, 987, 1094, 1137, 1724, 1861
MboI GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
MboII GAAGA 9 cut(s) 34, 40, 70, 105, 184, 203, 1066, 1295, 1851
MfeI CAATTG 2 cut(s) 854, 1794
MflI RGATCY 1 cut(s) 58
MhlI GDGCHC 5 cut(s) 982, 1149, 1454, 1508, 1702
MlsI TGGCCA 2 cut(s) 229, 550
MluI ACGCGT 1 cut(s) 906
MluNI TGGCCA 2 cut(s) 229, 550
MlyI GAGTC 3 cut(s) 155, 1571, 2065
MmeI TCCRAC 2 cut(s) 955, 1837
Mox20I TGGCCA 2 cut(s) 229, 550
Mph1103I ATGCAT 2 cut(s) 946, 2014
MroI TCCGGA 1 cut(s) 1463
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 1961
MscI TGGCCA 2 cut(s) 229, 550
MslI CAYNNNNRTG 2 cut(s) 719, 1442
Msp20I TGGCCA 2 cut(s) 229, 550
MspA1I CMGCKG 1 cut(s) 734
MspCI CTTAAG 1 cut(s) 244
MspI CCGG 6 cut(s) 112, 375, 1411, 1434, 1464, 1649
MspR9I CCNGG 8 cut(s) 112, 465, 756, 1344, 1412, 1649, 1650, 1866
MunI CAATTG 2 cut(s) 854, 1794
MvaI CCWGG 4 cut(s) 465, 756, 1344, 1866
MvnI CGCG 2 cut(s) 408, 908
MwoI GCNNNNNNNGC 9 cut(s) 43, 371, 527, 602, 926, 935, 1073, 1267, 1613
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 4 cut(s) 112, 1412, 1649, 1650
NcoI CCATGG 1 cut(s) 779
NdeII GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
NgoMIV GCCGGC 1 cut(s) 374
NheI GCTAGC 1 cut(s) 800
NmeAIII GCCGAG 1 cut(s) 1843
NmuCI GTSAC 2 cut(s) 614, 1825
NruI TCGCGA 1 cut(s) 408
NsiI ATGCAT 2 cut(s) 946, 2014
NspI RCATGY 1 cut(s) 1187
PaeR7I CTCGAG 1 cut(s) 1701
PagI TCATGA 1 cut(s) 1530
PaqCI CACCTGC 1 cut(s) 1067
PciI ACATGT 1 cut(s) 1183
PciSI GCTCTTC 1 cut(s) 1049
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 1961
PfeI GAWTC 2 cut(s) 151, 233
PflFI GACNNNGTC 1 cut(s) 620
PfoI TCCNGGA 1 cut(s) 463
PleI GAGTC 3 cut(s) 155, 1571, 2064
PpsI GAGTC 3 cut(s) 155, 1571, 2064
Ppu21I YACGTR 1 cut(s) 1872
PpuMI RGGWCCY 2 cut(s) 263, 690
PscI ACATGT 1 cut(s) 1183
PsiI TTATAA 1 cut(s) 1662
Psp5II RGGWCCY 2 cut(s) 263, 690
Psp6I CCWGG 4 cut(s) 463, 754, 1342, 1864
PspFI CCCAGC 2 cut(s) 1507, 1789
PspGI CCWGG 4 cut(s) 463, 754, 1342, 1864
PspPI GGNCC 7 cut(s) 263, 377, 690, 746, 1295, 1382, 1645
PspPPI RGGWCCY 2 cut(s) 263, 690
PspXI VCTCGAGB 1 cut(s) 1701
PstI CTGCAG 2 cut(s) 925, 1065
PstNI CAGNNNCTG 1 cut(s) 1082
PsuI RGATCY 1 cut(s) 58
PsyI GACNNNGTC 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 734
RruI TCGCGA 1 cut(s) 408
RsaI GTAC 2 cut(s) 347, 1874
RsaNI GTAC 2 cut(s) 346, 1873
RseI CAYNNNNRTG 2 cut(s) 719, 1442
SapI GCTCTTC 1 cut(s) 1049
Sau3AI GATC 9 cut(s) 58, 425, 811, 912, 985, 1092, 1135, 1722, 1859
Sau96I GGNCC 7 cut(s) 263, 377, 690, 746, 1295, 1382, 1645
SbfI CCTGCAGG 1 cut(s) 1065
SchI GAGTC 3 cut(s) 155, 1571, 2065
ScrFI CCNGG 8 cut(s) 112, 465, 756, 1344, 1412, 1649, 1650, 1866
SdaI CCTGCAGG 1 cut(s) 1065
SduI GDGCHC 5 cut(s) 982, 1149, 1454, 1508, 1702
SfaNI GCATC 7 cut(s) 40, 592, 916, 925, 931, 953, 1560
SfcI CTRYAG 3 cut(s) 921, 1061, 1083
Sfr274I CTCGAG 1 cut(s) 1701
SinI GGWCC 4 cut(s) 263, 690, 746, 1645
SlaI CTCGAG 1 cut(s) 1701
SmaI CCCGGG 1 cut(s) 1650
SmiMI CAYNNNNRTG 2 cut(s) 719, 1442
SmlI CTYRAG 3 cut(s) 244, 516, 1701
SmoI CTYRAG 3 cut(s) 244, 516, 1701
Sse8387I CCTGCAGG 1 cut(s) 1065
SsiI CCGC 2 cut(s) 449, 1268
SspMI CTAG 8 cut(s) 47, 342, 801, 990, 1772, 1836, 1923, 2080
StyD4I CCNGG 8 cut(s) 110, 463, 754, 1342, 1410, 1647, 1648, 1864
StyI CCWWGG 4 cut(s) 501, 779, 989, 1983
TaaI ACNGT 5 cut(s) 403, 620, 763, 793, 1831
TaiI ACGT 1 cut(s) 1874
TaqI TCGA 6 cut(s) 576, 624, 814, 984, 1024, 1702
TfiI GAWTC 2 cut(s) 151, 233
TscAI CASTG 4 cut(s) 406, 922, 955, 2073
TseFI GTSAC 2 cut(s) 614, 1825
Tsp45I GTSAC 2 cut(s) 614, 1825
TspDTI ATGAA 6 cut(s) 267, 305, 1056, 1275, 1578, 1728
TspMI CCCGGG 1 cut(s) 1648
TspRI CASTG 4 cut(s) 406, 922, 955, 2073
Tth111I GACNNNGTC 1 cut(s) 620
Vha464I CTTAAG 1 cut(s) 244
VpaK11BI GGWCC 4 cut(s) 263, 690, 746, 1645
XagI CCTNNNNNAGG 1 cut(s) 86
XapI RAATTY 4 cut(s) 121, 963, 1687, 1934
XceI RCATGY 1 cut(s) 1187
XcmI CCANNNNNNNNNTGG 1 cut(s) 1216
XhoI CTCGAG 1 cut(s) 1701
XmaI CCCGGG 1 cut(s) 1648
XmaJI CCTAGG 1 cut(s) 989
XmnI GAANNNNTTC 1 cut(s) 1961
XspI CTAG 8 cut(s) 47, 342, 801, 990, 1772, 1836, 1923, 2080
Zsp2I ATGCAT 2 cut(s) 946, 2014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.