Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000923

Physical Location & Seq

Genomic Coordinates Forward (+)

3128388 .. 3133735

Gene Structure 5348 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1039300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2322 bp

ATGGGAAATTCAACTCTCCCTCCTTTTCTTCTCTCAGTTCTTCTCTTCTCTTTGTTTCAGATTCCAACATATGCTGCCAAAAATTCTTACATTGTGTACTTAGGAGCACGTCCTCATGTTCTGGATCCTTCGTCTTCTGACCTCGATAGCGTGACAAATTCTCACTACAATCTGCTTGGAACAGTTTTGGGAAGCAATGAAAGGGCACAAGAAGCAATATTTTATTCATATACGAGAAATATCAATGGCTTTGCTGCAATTTTGGATGATGAAGAGGCTGTTCAGATTGAAAAGGATCCAAATGTTGTATCTGTCTTTCCAAACAGAGGAAGAAAACTACACACAACTCGTTCATGGGATTTTCTTGGATTGGAAGAAAACGGCGAAACCCGGCCCGGTTCCATTTTGAAGAAGGCACGGTTTGGTGCAAATACGATTATAGGAAACCTTGATACTGGTGTTTGGCCGGAATCAAAGAGCTTTAGCGATGAAGGGATGGGACCAATCCCATCTAAATGGCGTGGAATCTGTCAGCTCACCAAAAATGGTTCTCGTTGCAATAGGAAGCTGATCGGAGCAAGGTACTTCAGCAAAGGATACCTCGCGTATGCTTCCATGGTTAACTCCACTGCAGCCAAGTCCATCCAGCCCAACGCTCGTGACTATGCCGGTCATGGCTCACACACCCTTTCAACGGCTGGTGGTAATTTTGTTCCTAGAGCAAGTGTTTTCGGTAATGGCAATGGCACTGCAAAGGGTGGATCCCCAAAAGCTCGGGTGGCTGCGTACAAAGTATGCTGGCCGCCAATCAATGACAACGAGTGCTTTGATGCAGACATTTTGGCCGCTTTTGAAGCTGCAATTAGTGACGGTGTTGATGTGCTTTCAGTGTCTCTTGGTGGTGAAGCTGTAGAGTTTTTTAATGATGGGATTGCAATAGGGTCCTTCCATGCTGTTAAGAAGGGGATTACTGTGGTCAGCTCAGCTGGCAATTCAGGCCCTACTCCTGGGTCAGTGTCGAATGTGGCACCGTGGATGCTCACAGTTGGAGCTAGCACGATAGATCGCGAGTTCTCCAATTATGTTGCTCTTGGCAACAAGAAGCATCTCAAGGGAGCAAGTCTTTCCTCCACAGGTTTGCCAGCTGAAAAGTTCTACCCATTGATAAGTGCAAGCGATGCTAAAGCTACTAATGCGTCTGCTTCTGAAGCCCAGCTCTGCAAACCTAGTACCCTTGATAAAAAGAAGGCGGAGGGTAAAATTTTGGTTTGCGTTCGAGGGGAAAATGCAAGAGCCAACAAGGGTCAACAAGCTATTCTTGCAGGCGCTGTCGGAATGATTTTGGTCAATGATAAGCTAAGCGGGAATGAAATCATAGCTGATCCTCACTTGCTTCCGGCTTCACACGTTAATTTTTCCGACGGAGAATCTGTCTTTGCCTACATCAAGTCGACCAAGATTCCTATGGCTTACATTACTCGTGTAAAGACTGAACTAGGAACAAAACCAGCTCCATTTATGGCTTCATTCTCTTCGAGGGGGCCTAACCCCGTTGAGCAGTCAATTCTTAAGCCTGATATCACAGCGCCTGGAGTGAGTATAATTGCTGCTTATACTCAAGCAACTGGGCCAACTGATGGGGAGTTCGATACACGGCGTGTACCTTTCAATACAGAATCCGGAACTTCCATGTCGTGCCCTCATGTATCTGGAATTGTCGGTCTTTTGAAAACCCTTCACCCGACATGGACCCCTGCAGCTATTAAATCTGCAATCATGACCACCGCAAGAAAACGAGATAACAACAAGGGCACAATGCTGGACTCATCCAAGGCCAGAGCAACCCCATTTGCTTATGGTGCAGGACATGTTCAACCAAACAGTGCGATGGACCCTGGACTTGTTTATGACCTAACAACCGATGATTACTTGAACTTCTTATGTGCTCGTGGCTACAATGCAACACTTCTCAAAGTGTTTTCTAAAGAGCCACATAAATGTCCCAAGGCATATAGTCTTTCTGATTTTAACTATCCTTCTATAACAGTTCCCAATCTCCGTGACACGCCAGTGACTGTGACTAGAAGGGTTAAGAATGTTGGTTCTCCAGGCACGTACGTAGTTCGTGTTAAGGAACCGGTGGGAGTTTCTGTTACTGTTAAGCCTGGTACCTTGCAGTTCAAGAGCAATGGCGAAGAGAAGAAGTTCACGGTTGTTCTGAAGGCTCAGGTTCAAGGGCCTCAAGACTACGTATTTGGAGAGTTAAATTGGACGGATGGGAAGCACAATGTCAGGAGTCCTATTGTCGTCATGCACTACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

774

Amino Acids

82.75

Weight (kDa)

9.17

Isoelectric Point (pI)

27.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	29 - 114	2.3e-17	Peptidase inhibitor I9
MORF_dom	PF21864	50 - 109	2.2e-06	Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8	PF00082	142 - 605	5.1e-39	Subtilase family
PA	PF02225	404 - 477	4.5e-12	PA domain
fn3_6	PF17766	675 - 770	3.5e-30	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000923)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59810
fragaria_vesca	FvH4_3g00161 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42040 FvH4_3g42041 FvH4_3g42350
malus_domestica	MD03G1039300.v1.1 MD10G1341300.v1.1 MD11G1039600.v1.1 MD14G1139100.v1.1
prunus_persica	Prupe.4G001800_v2.0.a1 Prupe.6G031400_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1 Prupe.6G031500_v2.0.a1
pyrus_communis	pycom03g03030 pycom11g03350 pycom11g03360 pycom11g03370 pycom17g11100
rosa_chinensis	RchiOBHm_Chr5g0000221 RchiOBHm_Chr5g0075241 RchiOBHm_Chr5g0075251
rosa_laevigata	RLG00000030851 RLG00000036541 RLG00000036542
rosa_multiflora	Rmu_co8057730.1_g000001 Rmu_co8132088.1_g000001 Rmu_sc0000770.1_g000063 Rmu_sc0021936.1_g000001 Rmu_sc0031527.1_g000001 Rmu_ssc0000243.1_g000009
rosa_roxburghii	Rroxscaffold_1G00006310 Rroxscaffold_1G00075880 Rroxscaffold_3G00267190
rosa_rugosa	Rorug04G0382600 Rorug05G0437400 Rorug05G0437500 Rorug05G0437600 Rorug05G0437700 Rorug05G0437800
rosa_samantha	Rh5AG001500 Rh5AG494500 Rh5AG494600 Rh5BG002900 Rh5BG516100 Rh5BG516200 Rh5CG001800 Rh5CG539900 Rh5CG540000 Rh5DG001700 Rh5DG528800 Rh5DG528900
rosa_wichuraiana	Rw5G000200 Rw5G045880 Rw5G045890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	2168
AccB1I	GGYRCC	2 cut(s)	1027, 2168
AccB7I	CCANNNNNTGG	1 cut(s)	1637
AccI	GTMKAC	1 cut(s)	1451
AccII	CGCG	2 cut(s)	605, 1068
AccIII	TCCGGA	1 cut(s)	1679
AciI	CCGC	5 cut(s)	803, 846, 1250, 1362, 1785
AclWI	GGATC	7 cut(s)	119, 132, 290, 303, 756, 769, 1376
AcoI	YGGCCR	3 cut(s)	464, 800, 843
AcsI	RAATTY	4 cut(s)	7, 82, 157, 1260
AcuI	CTGAAG	3 cut(s)	571, 1227, 2240
AdeI	CACNNNGTG	1 cut(s)	1658
AfaI	GTAC	7 cut(s)	98, 584, 788, 1231, 1662, 2117, 2170
AfiI	CCNNNNNNNGG	4 cut(s)	326, 694, 1007, 1637
AflII	CTTAAG	1 cut(s)	1568
AflIII	ACRYGT	2 cut(s)	1405, 1867
AgeI	ACCGGT	1 cut(s)	2137
AjiI	CACGTC	1 cut(s)	110
AjnI	CCWGG	5 cut(s)	1006, 1588, 1894, 2107, 2164
AleI	CACNNNNGTG	1 cut(s)	2069
Alw21I	GWGCWC	2 cut(s)	109, 1948
Alw26I	GTCTC	1 cut(s)	897
AlwI	GGATC	7 cut(s)	119, 132, 290, 303, 756, 769, 1376
AlwNI	CAGNNNCTG	3 cut(s)	1328, 1589, 2075
Ama87I	CYCGRG	1 cut(s)	774
Aor13HI	TCCGGA	1 cut(s)	1679
AoxI	GGCC	9 cut(s)	392, 464, 800, 843, 997, 1541, 1628, 1833, 2237
ApeKI	GCWGC	7 cut(s)	74, 254, 632, 782, 857, 1607, 1757
ApoI	RAATTY	4 cut(s)	7, 82, 157, 1260
ArsI	GACNNNNNNTTYG	2 cut(s)	2237, 2269
AsiGI	ACCGGT	1 cut(s)	2137
Asp700I	GAANNNNTTC	1 cut(s)	2204
Asp718I	GGTACC	1 cut(s)	2168
AspLEI	GCGC	2 cut(s)	1328, 1588
AspS9I	GGNCC	9 cut(s)	393, 500, 942, 998, 1541, 1628, 1749, 1891, 2237
AsuC2I	CCSGG	2 cut(s)	391, 396
AsuHPI	GGTGA	3 cut(s)	529, 914, 1730
AsuNHI	GCTAGC	1 cut(s)	1052
AvaI	CYCGRG	1 cut(s)	774
AvaII	GGWCC	4 cut(s)	500, 942, 1749, 1891
BaeGI	GKGCMC	3 cut(s)	208, 1700, 1814
BaeI	ACNNNNGTAYC	2 cut(s)	444, 477
BamHI	GGATCC	3 cut(s)	124, 295, 761
BanI	GGYRCC	2 cut(s)	1027, 2168
BauI	CACGAG	3 cut(s)	657, 1479, 1947
BbsI	GAAGAC	1 cut(s)	126
Bbv12I	GWGCWC	2 cut(s)	109, 1948
BbvI	GCAGC	7 cut(s)	61, 241, 644, 769, 844, 1594, 1769
BccI	CCATC	7 cut(s)	490, 517, 650, 920, 1631, 1882, 2270
BceAI	ACGGC	3 cut(s)	397, 711, 1670
BciT130I	CCWGG	5 cut(s)	1008, 1590, 1896, 2109, 2166
BciVI	GTATCC	1 cut(s)	590
BcnI	CCSGG	2 cut(s)	391, 396
BcoDI	GTCTC	1 cut(s)	897
BfaI	CTAG	6 cut(s)	717, 1053, 1227, 1496, 2082, 2320
BfmI	CTRYAG	3 cut(s)	630, 909, 1755
BfoI	RGCGCY	2 cut(s)	1329, 1589
BfrI	CTTAAG	1 cut(s)	1568
BfuI	GTATCC	1 cut(s)	590
BisI	GCNGC	9 cut(s)	75, 255, 633, 783, 803, 846, 858, 1608, 1758
BlpI	GCTNAGC	2 cut(s)	982, 1358
BlsI	GCNGC	9 cut(s)	76, 256, 634, 784, 804, 847, 859, 1609, 1759
Bme1390I	CCNGG	7 cut(s)	391, 396, 1008, 1590, 1896, 2109, 2166
Bme18I	GGWCC	4 cut(s)	500, 942, 1749, 1891
BmeT110I	CYCGRG	1 cut(s)	774
BmgBI	CACGTC	1 cut(s)	110
BmgT120I	GGNCC	9 cut(s)	393, 500, 942, 998, 1541, 1628, 1749, 1891, 2237
BmrFI	CCNGG	7 cut(s)	391, 396, 1008, 1590, 1896, 2109, 2166
BmrI	ACTGGG	1 cut(s)	1635
BmsI	GCATC	4 cut(s)	820, 1026, 1114, 1168
BmtI	GCTAGC	1 cut(s)	1056
BmuI	ACTGGG	1 cut(s)	1635
BpiI	GAAGAC	1 cut(s)	126
BpmI	CTGGAG	2 cut(s)	1611, 2091
Bpu10I	CCTNAGC	1 cut(s)	2226
Bpu1102I	GCTNAGC	2 cut(s)	982, 1358
BpuEI	CTTGAG	3 cut(s)	1094, 1602, 2226
BpuMI	CCSGG	2 cut(s)	391, 396
BsaAI	YACGTR	3 cut(s)	2115, 2119, 2251
BsaJI	CCNNGG	6 cut(s)	615, 1007, 1031, 1830, 1894, 2004
BsaWI	WCCGGW	2 cut(s)	1679, 2137
BsaXI	ACNNNNNCTCC	5 cut(s)	34, 1416, 1446, 2136, 2166
Bsc4I	CCNNNNNNNGG	4 cut(s)	326, 694, 1007, 1637
Bse118I	RCCGGY	2 cut(s)	668, 2137
Bse1I	ACTGG	3 cut(s)	460, 1630, 2069
Bse3DI	GCAATG	3 cut(s)	202, 748, 2194
BseAI	TCCGGA	1 cut(s)	1679
BseBI	CCWGG	5 cut(s)	1008, 1590, 1896, 2109, 2166
BseDI	CCNNGG	6 cut(s)	615, 1007, 1031, 1830, 1894, 2004
BseGI	GGATG	6 cut(s)	271, 501, 642, 1041, 1826, 2281
BseLI	CCNNNNNNNGG	4 cut(s)	326, 694, 1007, 1637
BseMI	GCAATG	3 cut(s)	202, 748, 2194
BseMII	CTCAG	3 cut(s)	48, 996, 2240
BseNI	ACTGG	3 cut(s)	460, 1630, 2069
BseSI	GKGCMC	3 cut(s)	208, 1700, 1814
BseXI	GCAGC	7 cut(s)	61, 241, 644, 769, 844, 1594, 1769
BseYI	CCCAGC	1 cut(s)	1212
BsgI	GTGCAG	1 cut(s)	1881
Bsh1236I	CGCG	2 cut(s)	605, 1068
BshFI	GGCC	9 cut(s)	394, 466, 802, 845, 999, 1543, 1630, 1835, 2239
BshNI	GGYRCC	2 cut(s)	1027, 2168
BshTI	ACCGGT	1 cut(s)	2137
BsiHKAI	GWGCWC	2 cut(s)	109, 1948
BsiHKCI	CYCGRG	1 cut(s)	774
BsiSI	CCGG	7 cut(s)	391, 396, 467, 669, 1397, 1680, 2138
BsiWI	CGTACG	1 cut(s)	2115
BslFI	GGGAC	2 cut(s)	513, 1986
BslI	CCNNNNNNNGG	4 cut(s)	326, 694, 1007, 1637
BsmAI	GTCTC	1 cut(s)	897
BsmFI	GGGAC	2 cut(s)	513, 1986
BsnI	GGCC	9 cut(s)	394, 466, 802, 845, 999, 1543, 1630, 1835, 2239
BsoBI	CYCGRG	1 cut(s)	774
Bsp1286I	GDGCHC	5 cut(s)	109, 208, 1700, 1814, 1948
Bsp13I	TCCGGA	1 cut(s)	1679
Bsp143I	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
Bsp1720I	GCTNAGC	2 cut(s)	982, 1358
Bsp19I	CCATGG	1 cut(s)	615
Bsp68I	TCGCGA	1 cut(s)	1068
BspACI	CCGC	5 cut(s)	803, 846, 1250, 1362, 1785
BspANI	GGCC	9 cut(s)	394, 466, 802, 845, 999, 1543, 1630, 1835, 2239
BspCNI	CTCAG	3 cut(s)	47, 995, 2239
BspEI	TCCGGA	1 cut(s)	1679
BspFNI	CGCG	2 cut(s)	605, 1068
BspHI	TCATGA	1 cut(s)	1776
BspMAI	CTGCAG	2 cut(s)	634, 1759
BspOI	GCTAGC	1 cut(s)	1056
BspPI	GGATC	7 cut(s)	119, 132, 290, 303, 756, 769, 1376
BspT107I	GGYRCC	2 cut(s)	1027, 2168
BspTI	CTTAAG	1 cut(s)	1568
BsrDI	GCAATG	3 cut(s)	202, 748, 2194
BsrFI	RCCGGY	2 cut(s)	668, 2137
BsrI	ACTGG	3 cut(s)	460, 1630, 2069
BssAI	RCCGGY	2 cut(s)	668, 2137
BssECI	CCNNGG	6 cut(s)	615, 1007, 1031, 1830, 1894, 2004
BssMI	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
BssSI	CACGAG	3 cut(s)	657, 1479, 1947
BssT1I	CCWWGG	3 cut(s)	615, 1830, 2004
Bst2BI	CACGAG	3 cut(s)	657, 1479, 1947
Bst2UI	CCWGG	5 cut(s)	1008, 1590, 1896, 2109, 2166
Bst6I	CTCTTC	4 cut(s)	50, 267, 1537, 2190
BstAFI	CTTAAG	1 cut(s)	1568
BstAPI	GCANNNNNTGC	1 cut(s)	1178
BstBAI	YACGTR	3 cut(s)	2115, 2119, 2251
BstC8I	GCNNGC	6 cut(s)	800, 988, 1054, 1143, 1174, 1324
BstDEI	CTNAG	5 cut(s)	34, 100, 982, 1358, 2226
BstDSI	CCRYGG	2 cut(s)	615, 1031
BstF5I	GGATG	6 cut(s)	271, 501, 642, 1041, 1826, 2281
BstFNI	CGCG	2 cut(s)	605, 1068
BstH2I	RGCGCY	2 cut(s)	1329, 1589
BstHHI	GCGC	2 cut(s)	1328, 1588
BstKTI	GATC	6 cut(s)	127, 298, 573, 764, 1066, 1384
BstMAI	GTCTC	1 cut(s)	897
BstMBI	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
BstNI	CCWGG	5 cut(s)	1008, 1590, 1896, 2109, 2166
BstNSI	RCATGY	1 cut(s)	1871
BstSCI	CCNGG	7 cut(s)	389, 394, 1006, 1588, 1894, 2107, 2164
BstSFI	CTRYAG	3 cut(s)	630, 909, 1755
BstSLI	GKGCMC	3 cut(s)	208, 1700, 1814
BstSNI	TACGTA	2 cut(s)	2119, 2251
BstUI	CGCG	2 cut(s)	605, 1068
BstV1I	GCAGC	7 cut(s)	61, 241, 644, 769, 844, 1594, 1769
BstV2I	GAAGAC	1 cut(s)	126
BstX2I	RGATCY	3 cut(s)	124, 295, 761
BstXI	CCANNNNNNTGG	1 cut(s)	516
BstYI	RGATCY	3 cut(s)	124, 295, 761
BsuI	GTATCC	1 cut(s)	590
BsuRI	GGCC	9 cut(s)	394, 466, 802, 845, 999, 1543, 1630, 1835, 2239
BtgI	CCRYGG	2 cut(s)	615, 1031
BtgZI	GCGATG	3 cut(s)	501, 1191, 1901
BtrI	CACGTC	1 cut(s)	110
BtsCI	GGATG	6 cut(s)	271, 501, 642, 1041, 1826, 2281
BtsI	GCAGTG	2 cut(s)	627, 747
BtsIMutI	CAGTG	6 cut(s)	627, 747, 894, 1020, 1888, 2076
BtuMI	TCGCGA	1 cut(s)	1068
Cac8I	GCNNGC	6 cut(s)	800, 988, 1054, 1143, 1174, 1324
CaiI	CAGNNNCTG	3 cut(s)	1328, 1589, 2075
CciI	TCATGA	1 cut(s)	1776
CfoI	GCGC	2 cut(s)	1328, 1588
Cfr10I	RCCGGY	2 cut(s)	668, 2137
Cfr13I	GGNCC	9 cut(s)	393, 500, 942, 998, 1541, 1628, 1749, 1891, 2237
CseI	GACGC	1 cut(s)	1185
Csp6I	GTAC	7 cut(s)	97, 583, 787, 1230, 1661, 2116, 2169
CspAI	ACCGGT	1 cut(s)	2137
CviQI	GTAC	7 cut(s)	97, 583, 787, 1230, 1661, 2116, 2169
DdeI	CTNAG	5 cut(s)	34, 100, 982, 1358, 2226
DpnI	GATC	6 cut(s)	126, 297, 572, 763, 1065, 1383
DpnII	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
DraIII	CACNNNGTG	1 cut(s)	1658
EaeI	YGGCCR	3 cut(s)	464, 800, 843
Eam1104I	CTCTTC	4 cut(s)	50, 267, 1537, 2190
EarI	CTCTTC	4 cut(s)	50, 267, 1537, 2190
EciI	GGCGGA	1 cut(s)	1265
Eco105I	TACGTA	2 cut(s)	2119, 2251
Eco130I	CCWWGG	3 cut(s)	615, 1830, 2004
Eco32I	GATATC	1 cut(s)	1579
Eco47I	GGWCC	4 cut(s)	500, 942, 1749, 1891
Eco57I	CTGAAG	3 cut(s)	571, 1227, 2240
Eco88I	CYCGRG	1 cut(s)	774
EcoO109I	RGGNCCY	4 cut(s)	942, 998, 1541, 2237
EcoRII	CCWGG	5 cut(s)	1006, 1588, 1894, 2107, 2164
EcoRV	GATATC	1 cut(s)	1579
EcoT14I	CCWWGG	3 cut(s)	615, 1830, 2004
ErhI	CCWWGG	3 cut(s)	615, 1830, 2004
FalI	AAGNNNNNCTT	2 cut(s)	1302, 1334
FaqI	GGGAC	2 cut(s)	513, 1986
FauI	CCCGC	1 cut(s)	1355
FauNDI	CATATG	1 cut(s)	70
FblI	GTMKAC	1 cut(s)	1451
Fnu4HI	GCNGC	9 cut(s)	75, 255, 633, 783, 803, 846, 858, 1608, 1758
FokI	GGATG	6 cut(s)	278, 508, 629, 1048, 1813, 2288
Fsp4HI	GCNGC	9 cut(s)	75, 255, 633, 783, 803, 846, 858, 1608, 1758
FspBI	CTAG	6 cut(s)	717, 1053, 1227, 1496, 2082, 2320
GlaI	GCGC	2 cut(s)	1327, 1587
GluI	GCNGC	9 cut(s)	75, 255, 633, 783, 803, 846, 858, 1608, 1758
GsaI	CCCAGC	1 cut(s)	1216
GsuI	CTGGAG	2 cut(s)	1611, 2091
HaeII	RGCGCY	2 cut(s)	1329, 1589
HaeIII	GGCC	9 cut(s)	394, 466, 802, 845, 999, 1543, 1630, 1835, 2239
HapII	CCGG	7 cut(s)	391, 396, 467, 669, 1397, 1680, 2138
HgaI	GACGC	1 cut(s)	1185
HhaI	GCGC	2 cut(s)	1328, 1588
Hin6I	GCGC	2 cut(s)	1326, 1586
HinP1I	GCGC	2 cut(s)	1326, 1586
HincII	GTYRAC	3 cut(s)	622, 1307, 1452
HindII	GTYRAC	3 cut(s)	622, 1307, 1452
HinfI	GANTC	8 cut(s)	61, 470, 525, 1427, 1459, 1676, 1823, 2296
HpaI	GTTAAC	1 cut(s)	622
HpaII	CCGG	7 cut(s)	391, 396, 467, 669, 1397, 1680, 2138
HphI	GGTGA	3 cut(s)	529, 914, 1730
Hpy166II	GTNNAC	6 cut(s)	97, 622, 1307, 1452, 1661, 2208
Hpy188I	TCNGA	9 cut(s)	60, 139, 285, 575, 1207, 1334, 1420, 2023, 2220
Hpy188III	TCNNGA	9 cut(s)	122, 659, 1067, 1680, 1710, 1777, 2182, 2243, 2293
Hpy8I	GTNNAC	6 cut(s)	97, 622, 1307, 1452, 1661, 2208
Hpy99I	CGWCG	1 cut(s)	1424
HpyCH4IV	ACGT	5 cut(s)	109, 1407, 2114, 2118, 2250
HpyF3I	CTNAG	5 cut(s)	34, 100, 982, 1358, 2226
HpySE526I	ACGT	5 cut(s)	109, 1407, 2114, 2118, 2250
HspAI	GCGC	2 cut(s)	1326, 1586
Kpn2I	TCCGGA	1 cut(s)	1679
KpnI	GGTACC	1 cut(s)	2172
KspAI	GTTAAC	1 cut(s)	622
Kzo9I	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
LmnI	GCTCC	5 cut(s)	104, 575, 1049, 1115, 1516
Lsp1109I	GCAGC	7 cut(s)	61, 241, 644, 769, 844, 1594, 1769
LweI	GCATC	4 cut(s)	820, 1026, 1114, 1168
MaeI	CTAG	6 cut(s)	717, 1053, 1227, 1496, 2082, 2320
MaeII	ACGT	5 cut(s)	109, 1407, 2114, 2118, 2250
MaeIII	GTNAC	7 cut(s)	151, 659, 866, 2060, 2071, 2077, 2152
MalI	GATC	6 cut(s)	126, 297, 572, 763, 1065, 1383
MboI	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
MflI	RGATCY	3 cut(s)	124, 295, 761
MhlI	GDGCHC	5 cut(s)	109, 208, 1700, 1814, 1948
MlyI	GAGTC	2 cut(s)	1817, 2305
MmeI	TCCRAC	4 cut(s)	89, 1027, 1312, 1443
MroI	TCCGGA	1 cut(s)	1679
MroXI	GAANNNNTTC	1 cut(s)	2204
MslI	CAYNNNNRTG	3 cut(s)	514, 1996, 2069
MspA1I	CMGCKG	2 cut(s)	986, 1145
MspCI	CTTAAG	1 cut(s)	1568
MspI	CCGG	7 cut(s)	391, 396, 467, 669, 1397, 1680, 2138
MspR9I	CCNGG	7 cut(s)	391, 396, 1008, 1590, 1896, 2109, 2166
MvaI	CCWGG	5 cut(s)	1008, 1590, 1896, 2109, 2166
MvnI	CGCG	2 cut(s)	605, 1068
NciI	CCSGG	2 cut(s)	391, 396
NcoI	CCATGG	1 cut(s)	615
NdeI	CATATG	1 cut(s)	70
NdeII	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
NheI	GCTAGC	1 cut(s)	1052
NmuCI	GTSAC	6 cut(s)	151, 659, 866, 2060, 2071, 2077
NruI	TCGCGA	1 cut(s)	1068
NspI	RCATGY	1 cut(s)	1871
OliI	CACNNNNGTG	1 cut(s)	2069
PagI	TCATGA	1 cut(s)	1776
PciI	ACATGT	1 cut(s)	1867
PdmI	GAANNNNTTC	1 cut(s)	2204
PfeI	GAWTC	6 cut(s)	61, 470, 525, 1427, 1459, 1676
Pfl23II	CGTACG	1 cut(s)	2115
PflMI	CCANNNNNTGG	1 cut(s)	1637
PinAI	ACCGGT	1 cut(s)	2137
PkrI	GCNGC	9 cut(s)	76, 256, 634, 784, 804, 847, 859, 1609, 1759
PleI	GAGTC	2 cut(s)	1817, 2304
PpsI	GAGTC	2 cut(s)	1817, 2304
Ppu21I	YACGTR	3 cut(s)	2115, 2119, 2251
PpuMI	RGGWCCY	1 cut(s)	942
PscI	ACATGT	1 cut(s)	1867
Psp5II	RGGWCCY	1 cut(s)	942
Psp6I	CCWGG	5 cut(s)	1006, 1588, 1894, 2107, 2164
PspFI	CCCAGC	1 cut(s)	1212
PspGI	CCWGG	5 cut(s)	1006, 1588, 1894, 2107, 2164
PspLI	CGTACG	1 cut(s)	2115
PspPI	GGNCC	9 cut(s)	393, 500, 942, 998, 1541, 1628, 1749, 1891, 2237
PspPPI	RGGWCCY	1 cut(s)	942
PstI	CTGCAG	2 cut(s)	634, 1759
PstNI	CAGNNNCTG	3 cut(s)	1328, 1589, 2075
PsuI	RGATCY	3 cut(s)	124, 295, 761
PvuII	CAGCTG	2 cut(s)	986, 1145
RruI	TCGCGA	1 cut(s)	1068
RsaI	GTAC	7 cut(s)	98, 584, 788, 1231, 1662, 2117, 2170
RsaNI	GTAC	7 cut(s)	97, 583, 787, 1230, 1661, 2116, 2169
RseI	CAYNNNNRTG	3 cut(s)	514, 1996, 2069
SalI	GTCGAC	1 cut(s)	1450
SatI	GCNGC	9 cut(s)	75, 255, 633, 783, 803, 846, 858, 1608, 1758
Sau3AI	GATC	6 cut(s)	124, 295, 570, 761, 1063, 1381
Sau96I	GGNCC	9 cut(s)	393, 500, 942, 998, 1541, 1628, 1749, 1891, 2237
SchI	GAGTC	2 cut(s)	1817, 2305
ScrFI	CCNGG	7 cut(s)	391, 396, 1008, 1590, 1896, 2109, 2166
SduI	GDGCHC	5 cut(s)	109, 208, 1700, 1814, 1948
SfaNI	GCATC	4 cut(s)	820, 1026, 1114, 1168
SfcI	CTRYAG	3 cut(s)	630, 909, 1755
SinI	GGWCC	4 cut(s)	500, 942, 1749, 1891
SmiMI	CAYNNNNRTG	3 cut(s)	514, 1996, 2069
SmlI	CTYRAG	4 cut(s)	1109, 1568, 1617, 2241
SmoI	CTYRAG	4 cut(s)	1109, 1568, 1617, 2241
SnaBI	TACGTA	2 cut(s)	2119, 2251
SsiI	CCGC	5 cut(s)	803, 846, 1250, 1362, 1785
SspI	AATATT	1 cut(s)	219
SspMI	CTAG	6 cut(s)	717, 1053, 1227, 1496, 2082, 2320
StyD4I	CCNGG	7 cut(s)	389, 394, 1006, 1588, 1894, 2107, 2164
StyI	CCWWGG	3 cut(s)	615, 1830, 2004
TaiI	ACGT	5 cut(s)	112, 1410, 2117, 2121, 2253
TaqI	TCGA	6 cut(s)	144, 1019, 1276, 1451, 1535, 1647
TaqII	GACCGA	1 cut(s)	1709
TatI	WGTACW	1 cut(s)	96
TauI	GCSGC	2 cut(s)	805, 848
TfiI	GAWTC	6 cut(s)	61, 470, 525, 1427, 1459, 1676
TscAI	CASTG	6 cut(s)	634, 754, 894, 1020, 1888, 2076
TseFI	GTSAC	6 cut(s)	151, 659, 866, 2060, 2071, 2077
TseI	GCWGC	7 cut(s)	74, 254, 632, 782, 857, 1607, 1757
Tsp45I	GTSAC	6 cut(s)	151, 659, 866, 2060, 2071, 2077
TspDTI	ATGAA	7 cut(s)	213, 216, 285, 342, 504, 1383, 1515
TspGWI	ACGGA	3 cut(s)	1437, 2048, 2288
TspRI	CASTG	6 cut(s)	634, 754, 894, 1020, 1888, 2076
Van91I	CCANNNNNTGG	1 cut(s)	1637
Vha464I	CTTAAG	1 cut(s)	1568
VpaK11BI	GGWCC	4 cut(s)	500, 942, 1749, 1891
XapI	RAATTY	4 cut(s)	7, 82, 157, 1260
XceI	RCATGY	1 cut(s)	1871
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1462
XmiI	GTMKAC	1 cut(s)	1451
XmnI	GAANNNNTTC	1 cut(s)	2204
XspI	CTAG	6 cut(s)	717, 1053, 1227, 1496, 2082, 2320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.