Rh5BG516100

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000923
Physical Location & Seq
Reverse (-)
81734152 .. 81738558
4407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG516100.1

Sequence Viewer

Length: 1485 bp
ATGGTGCTTTCATCTCTACCTCCATTACTATTATTATCAACTCTTTTGTTCTCTCTGTTGCAGACACCTTCCCTTTCCATTAAAAAGCCATATATAGTGTACTTAGGAGCACATTCTCATGGTCCAAACCCATCATCTGTTGATCTCGATTTTGTCAGAAATTTTCACTACAATTTTCTGGGGTCATTCTTGAGAAGCAATAAAAGTGCCAAAGATGTGATGTTCTACTCCTATACTAGACACATTAATGGTTTTGCTGCAATTCTTGAAGAGGAAGAAGCTGCTCAGATTGCTAAGGATCCAAATGTGATATCAGTCTTCCTAAACAAGGGAAGAAAGCTGCAAACTACCCGGTCATGGAATTTTCTTGGATTAGAGAGAAATGGACTGATTCCTTCAGACTCCATTTGGAAGAAAGCAAGGTTTGGCGAAGATACAATAATTGCAAACATAGATACTGGTGTTTGGCCAGAATCCAAAAGCTTTAGTGATGAAGGGCTGGGACCTGTCCCTTCCAAGTGGCGTGGAACTTGTCAACATGACACAAAGCAAGTTCATTGCAACAGAAAGCTGATTGGAACTAGATACTTTAATAATGGTCTTGCCATGTATGCCGGCCCTCTCAACTCGTCGTATTCCACTGTTCGTGACTACGATGGTCATGGATCACATACCCTATCCACGGCTGCAGGTGGCTTTGTTCCAGGAGTTAGTGTTTTTGGCAATGGCAATGGAACCACCAAGGGTGGCTCTCCTCAAGCCCGTGTTGCTGCATATAAGGTATGCTGGCCACCATTTGAAGGTGTTGGGTGCTTCGAGGCAGACATCTTAGCTGCCTTTGATGCTGCAATAAGTGACGGTGTCGATGTAATCTCTGTGTCTCTTGGTGGAGGAACAGAGGAGTTTTTCAACAATGCAATTTCAATAGGGTCCTTCCATGCAGTTAAAAATGGCATTGTTGTGGTTAGTGCAGCTGGAAACTCTGGACCAAATCCAGGGACAGTATTAAACTTGTCGCCATGGTTAGTAACAGTTGGTGCTAGCACCATTGATCGAGAGTTCACAAGCTATGTTGTCCTCGGAAACAAAAAGCAATTGAAGGGAGCAAGCCTTTCAGCTAAAGGCTTACCATTTGAAATGTTTTACCCGTTGATCAGTGCTGCAGATGCGAGAGATGCTGATGCATCCACTGGAAAAGCTGAAATTTGCAATGTTGGAGCTCTCGATCCTAGGAAAGTAAAAGGAAAGATTTTAGTGTGCGTTCGAGAATACAACGATAATGAAAGAACCGAGAAGAGCATGCAGGCCGATATTGCAGGTGCTGTAGGAATGATCTTGGTTAATGATAAGCAAAGTGGAAACGATGTTGTAGCCGATCCTCATGTGCTCCTTGTTTCACATCTCAATTACACCGACGGCAAACATGTCTTTGATTACATTAAATCTACCAAGTCTTTAATCTCTCACCCAATGATCTCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

494

Amino Acids

53.16

Weight (kDa)

7.66

Isoelectric Point (pI)

25.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 31 - 115 1.2e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 143 - 353 4.6e-16 Subtilase family
PA PF02225 395 - 474 2.7e-10 PA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 680, 1307
Acc36I ACCTGC 2 cut(s) 680, 1307
AclWI GGATC 5 cut(s) 293, 306, 673, 1220, 1370
AcoI YGGCCR 2 cut(s) 467, 788
AcsI RAATTY 3 cut(s) 160, 361, 1203
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 5 cut(s) 328, 357, 682, 800, 995
AflIII ACRYGT 1 cut(s) 1423
AgsI TTSAA 6 cut(s) 269, 800, 910, 924, 1099, 1136
AjnI CCWGG 2 cut(s) 703, 994
Alw21I GWGCWC 3 cut(s) 112, 1222, 1389
Alw26I GTCTC 1 cut(s) 885
AlwI GGATC 5 cut(s) 293, 306, 673, 1220, 1370
AlwNI CAGNNNCTG 1 cut(s) 1322
AoxI GGCC 4 cut(s) 467, 616, 788, 1305
ApeKI GCWGC 9 cut(s) 257, 281, 340, 686, 770, 833, 845, 971, 1160
ApoI RAATTY 3 cut(s) 160, 361, 1203
AseI ATTAAT 1 cut(s) 246
AspA2I CCTAGG 1 cut(s) 1229
AspS9I GGNCC 5 cut(s) 122, 503, 617, 930, 986
AsuC2I CCSGG 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 1457
AsuNHI GCTAGC 1 cut(s) 1040
AvaII GGWCC 4 cut(s) 122, 503, 930, 986
AvrII CCTAGG 1 cut(s) 1229
BaeI ACNNNNGTAYC 2 cut(s) 447, 480
BalI TGGCCA 2 cut(s) 469, 790
BamHI GGATCC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 1222
BbsI GAAGAC 1 cut(s) 310
Bbv12I GWGCWC 3 cut(s) 112, 1222, 1389
BbvI GCAGC 9 cut(s) 244, 268, 327, 673, 757, 820, 832, 983, 1147
BccI CCATC 2 cut(s) 139, 650
BceAI ACGGC 2 cut(s) 699, 1432
BciT130I CCWGG 2 cut(s) 705, 996
BclI TGATCA 1 cut(s) 1152
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 885
BfaI CTAG 4 cut(s) 237, 582, 1041, 1230
BfmI CTRYAG 3 cut(s) 687, 1161, 1323
BfuAI ACCTGC 2 cut(s) 680, 1307
BisI GCNGC 9 cut(s) 258, 282, 341, 687, 771, 834, 846, 972, 1161
BlnI CCTAGG 1 cut(s) 1229
BlsI GCNGC 9 cut(s) 259, 283, 342, 688, 772, 835, 847, 973, 1162
Bme1390I CCNGG 3 cut(s) 352, 705, 996
Bme18I GGWCC 4 cut(s) 122, 503, 930, 986
BmgT120I GGNCC 5 cut(s) 122, 503, 617, 930, 986
BmiI GGNNCC 4 cut(s) 300, 504, 736, 931
BmrFI CCNGG 3 cut(s) 352, 705, 996
BmsI GCATC 5 cut(s) 832, 1156, 1165, 1171, 1193
BmtI GCTAGC 1 cut(s) 1044
BpiI GAAGAC 1 cut(s) 310
Bpu10I CCTNAGC 1 cut(s) 294
BpuEI CTTGAG 2 cut(s) 211, 741
BpuMI CCSGG 1 cut(s) 352
BsaJI CCNNGG 6 cut(s) 681, 741, 995, 1019, 1078, 1229
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 5 cut(s) 328, 357, 682, 800, 995
Bse118I RCCGGY 1 cut(s) 614
Bse1I ACTGG 2 cut(s) 463, 1195
Bse3DI GCAATG 4 cut(s) 556, 730, 736, 1216
BseBI CCWGG 2 cut(s) 705, 996
BseDI CCNNGG 6 cut(s) 681, 741, 995, 1019, 1078, 1229
BseGI GGATG 1 cut(s) 1184
BseLI CCNNNNNNNGG 5 cut(s) 328, 357, 682, 800, 995
BseMI GCAATG 4 cut(s) 556, 730, 736, 1216
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 2 cut(s) 463, 1195
BseRI GAGGAG 2 cut(s) 744, 914
BseXI GCAGC 9 cut(s) 244, 268, 327, 673, 757, 820, 832, 983, 1147
BseYI CCCAGC 1 cut(s) 499
BsgI GTGCAG 1 cut(s) 990
BshFI GGCC 4 cut(s) 469, 618, 790, 1307
BsiHKAI GWGCWC 3 cut(s) 112, 1222, 1389
BsiSI CCGG 2 cut(s) 352, 615
BslFI GGGAC 3 cut(s) 494, 516, 1012
BslI CCNNNNNNNGG 5 cut(s) 328, 357, 682, 800, 995
BsmAI GTCTC 1 cut(s) 885
BsmFI GGGAC 3 cut(s) 494, 516, 1012
BsnI GGCC 4 cut(s) 469, 618, 790, 1307
Bsp1286I GDGCHC 3 cut(s) 112, 1222, 1389
Bsp143I GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
Bsp19I CCATGG 1 cut(s) 1019
BspANI GGCC 4 cut(s) 469, 618, 790, 1307
BspCNI CTCAG 1 cut(s) 298
BspLI GGNNCC 4 cut(s) 300, 504, 736, 931
BspMAI CTGCAG 2 cut(s) 691, 1165
BspMI ACCTGC 2 cut(s) 680, 1307
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 5 cut(s) 293, 306, 673, 1220, 1370
BspQI GCTCTTC 1 cut(s) 1289
BsrDI GCAATG 4 cut(s) 556, 730, 736, 1216
BsrFI RCCGGY 1 cut(s) 614
BsrI ACTGG 2 cut(s) 463, 1195
BssAI RCCGGY 1 cut(s) 614
BssECI CCNNGG 6 cut(s) 681, 741, 995, 1019, 1078, 1229
BssMI GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
BssT1I CCWWGG 3 cut(s) 741, 1019, 1229
Bst2UI CCWGG 2 cut(s) 705, 996
Bst4CI ACNGT 4 cut(s) 643, 860, 1003, 1033
Bst6I CTCTTC 2 cut(s) 264, 1289
BstC8I GCNNGC 6 cut(s) 616, 788, 1042, 1108, 1301, 1305
BstDEI CTNAG 4 cut(s) 103, 285, 294, 829
BstDSI CCRYGG 2 cut(s) 681, 1019
BstENI CCTNNNNNAGG 1 cut(s) 326
BstF5I GGATG 1 cut(s) 1184
BstKTI GATC 9 cut(s) 145, 301, 668, 1054, 1155, 1228, 1335, 1378, 1476
BstMAI GTCTC 1 cut(s) 885
BstMBI GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
BstMWI GCNNNNNNNGC 7 cut(s) 290, 611, 767, 842, 1166, 1175, 1313
BstNI CCWGG 2 cut(s) 705, 996
BstNSI RCATGY 2 cut(s) 1303, 1427
BstSCI CCNGG 3 cut(s) 350, 703, 994
BstSFI CTRYAG 3 cut(s) 687, 1161, 1323
BstV1I GCAGC 9 cut(s) 244, 268, 327, 673, 757, 820, 832, 983, 1147
BstV2I GAAGAC 1 cut(s) 310
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 4 cut(s) 469, 618, 790, 1307
BtgI CCRYGG 2 cut(s) 681, 1019
BtsCI GGATG 1 cut(s) 1184
BtsIMutI CAGTG 3 cut(s) 639, 1162, 1188
BveI ACCTGC 2 cut(s) 680, 1307
Cac8I GCNNGC 6 cut(s) 616, 788, 1042, 1108, 1301, 1305
CaiI CAGNNNCTG 1 cut(s) 1322
Cfr10I RCCGGY 1 cut(s) 614
Cfr13I GGNCC 5 cut(s) 122, 503, 617, 930, 986
Csp6I GTAC 1 cut(s) 100
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 4 cut(s) 103, 285, 294, 829
DpnI GATC 9 cut(s) 144, 300, 667, 1053, 1154, 1227, 1334, 1377, 1475
DpnII GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
EaeI YGGCCR 2 cut(s) 467, 788
Eam1104I CTCTTC 2 cut(s) 264, 1289
EarI CTCTTC 2 cut(s) 264, 1289
Ecl136II GAGCTC 1 cut(s) 1220
Eco130I CCWWGG 3 cut(s) 741, 1019, 1229
Eco24I GRGCYC 1 cut(s) 1222
Eco32I GATATC 1 cut(s) 312
Eco47I GGWCC 4 cut(s) 122, 503, 930, 986
Eco53kI GAGCTC 1 cut(s) 1220
Eco57I CTGAAG 1 cut(s) 381
EcoICRI GAGCTC 1 cut(s) 1220
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoO109I RGGNCCY 2 cut(s) 503, 930
EcoRII CCWGG 2 cut(s) 703, 994
EcoRV GATATC 1 cut(s) 312
EcoT14I CCWWGG 3 cut(s) 741, 1019, 1229
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 1 cut(s) 1222
ErhI CCWWGG 3 cut(s) 741, 1019, 1229
FaqI GGGAC 3 cut(s) 494, 516, 1012
FbaI TGATCA 1 cut(s) 1152
Fnu4HI GCNGC 9 cut(s) 258, 282, 341, 687, 771, 834, 846, 972, 1161
FokI GGATG 1 cut(s) 1171
FriOI GRGCYC 1 cut(s) 1222
Fsp4HI GCNGC 9 cut(s) 258, 282, 341, 687, 771, 834, 846, 972, 1161
FspBI CTAG 4 cut(s) 237, 582, 1041, 1230
GluI GCNGC 9 cut(s) 258, 282, 341, 687, 771, 834, 846, 972, 1161
GsaI CCCAGC 1 cut(s) 503
HaeIII GGCC 4 cut(s) 469, 618, 790, 1307
HapII CCGG 2 cut(s) 352, 615
HincII GTYRAC 1 cut(s) 536
HindII GTYRAC 1 cut(s) 536
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 3 cut(s) 391, 401, 473
HpaII CCGG 2 cut(s) 352, 615
HphI GGTGA 1 cut(s) 1457
Hpy166II GTNNAC 3 cut(s) 100, 536, 1062
Hpy188I TCNGA 4 cut(s) 158, 288, 400, 1082
Hpy188III TCNNGA 8 cut(s) 146, 190, 266, 647, 984, 1055, 1223, 1265
Hpy8I GTNNAC 3 cut(s) 100, 536, 1062
Hpy99I CGWCG 2 cut(s) 634, 1418
HpyAV CCTTC 7 cut(s) 78, 405, 488, 522, 794, 943, 1093
HpyCH4III ACNGT 4 cut(s) 643, 860, 1003, 1033
HpyF10VI GCNNNNNNNGC 7 cut(s) 290, 611, 767, 842, 1166, 1175, 1313
HpyF3I CTNAG 4 cut(s) 103, 285, 294, 829
KroI GCCGGC 1 cut(s) 614
KroNI GCCGGC 1 cut(s) 616
Ksp22I TGATCA 1 cut(s) 1152
Kzo9I GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
LguI GCTCTTC 1 cut(s) 1289
LmnI GCTCC 4 cut(s) 107, 1103, 1217, 1392
Lsp1109I GCAGC 9 cut(s) 244, 268, 327, 673, 757, 820, 832, 983, 1147
LweI GCATC 5 cut(s) 832, 1156, 1165, 1171, 1193
MaeI CTAG 4 cut(s) 237, 582, 1041, 1230
MaeIII GTNAC 3 cut(s) 647, 854, 1027
MalI GATC 9 cut(s) 144, 300, 667, 1053, 1154, 1227, 1334, 1377, 1475
MboI GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
MboII GAAGA 7 cut(s) 281, 287, 310, 345, 424, 443, 1306
MfeI CAATTG 1 cut(s) 1094
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 3 cut(s) 112, 1222, 1389
MlsI TGGCCA 2 cut(s) 469, 790
MluCI AATT 9 cut(s) 160, 172, 261, 361, 441, 918, 1094, 1203, 1405
MluNI TGGCCA 2 cut(s) 469, 790
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 1195
MnlI CCTC 9 cut(s) 30, 265, 630, 765, 811, 884, 892, 1088, 1389
Mox20I TGGCCA 2 cut(s) 469, 790
Mph1103I ATGCAT 1 cut(s) 1186
MroNI GCCGGC 1 cut(s) 614
MscI TGGCCA 2 cut(s) 469, 790
MseI TTAA 8 cut(s) 81, 246, 591, 945, 1007, 1341, 1440, 1457
MslI CAYNNNNRTG 3 cut(s) 117, 246, 959
Msp20I TGGCCA 2 cut(s) 469, 790
MspA1I CMGCKG 1 cut(s) 974
MspI CCGG 2 cut(s) 352, 615
MspR9I CCNGG 3 cut(s) 352, 705, 996
MunI CAATTG 1 cut(s) 1094
MvaI CCWGG 2 cut(s) 705, 996
MwoI GCNNNNNNNGC 7 cut(s) 290, 611, 767, 842, 1166, 1175, 1313
NaeI GCCGGC 1 cut(s) 616
NciI CCSGG 1 cut(s) 352
NcoI CCATGG 1 cut(s) 1019
NdeII GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
NgoMIV GCCGGC 1 cut(s) 614
NheI GCTAGC 1 cut(s) 1040
NlaIV GGNNCC 4 cut(s) 300, 504, 736, 931
NmuCI GTSAC 2 cut(s) 647, 854
NsiI ATGCAT 1 cut(s) 1186
NspI RCATGY 2 cut(s) 1303, 1427
PaeI GCATGC 1 cut(s) 1303
PaqCI CACCTGC 2 cut(s) 680, 1307
PciI ACATGT 1 cut(s) 1423
PciSI GCTCTTC 1 cut(s) 1289
PdiI GCCGGC 1 cut(s) 616
PfeI GAWTC 2 cut(s) 391, 473
PflFI GACNNNGTC 1 cut(s) 860
PfoI TCCNGGA 1 cut(s) 703
PkrI GCNGC 9 cut(s) 259, 283, 342, 688, 772, 835, 847, 973, 1162
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
PpuMI RGGWCCY 2 cut(s) 503, 930
PscI ACATGT 1 cut(s) 1423
PshBI ATTAAT 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 1222
Psp5II RGGWCCY 2 cut(s) 503, 930
Psp6I CCWGG 2 cut(s) 703, 994
PspFI CCCAGC 1 cut(s) 499
PspGI CCWGG 2 cut(s) 703, 994
PspN4I GGNNCC 4 cut(s) 300, 504, 736, 931
PspPI GGNCC 5 cut(s) 122, 503, 617, 930, 986
PspPPI RGGWCCY 2 cut(s) 503, 930
PstI CTGCAG 2 cut(s) 691, 1165
PstNI CAGNNNCTG 1 cut(s) 1322
PsuI RGATCY 1 cut(s) 298
PsyI GACNNNGTC 1 cut(s) 860
PvuII CAGCTG 1 cut(s) 974
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 3 cut(s) 117, 246, 959
SacI GAGCTC 1 cut(s) 1222
SapI GCTCTTC 1 cut(s) 1289
SaqAI TTAA 8 cut(s) 81, 246, 591, 945, 1007, 1341, 1440, 1457
SatI GCNGC 9 cut(s) 258, 282, 341, 687, 771, 834, 846, 972, 1161
Sau3AI GATC 9 cut(s) 142, 298, 665, 1051, 1152, 1225, 1332, 1375, 1473
Sau96I GGNCC 5 cut(s) 122, 503, 617, 930, 986
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 3 cut(s) 352, 705, 996
SduI GDGCHC 3 cut(s) 112, 1222, 1389
SfaNI GCATC 5 cut(s) 832, 1156, 1165, 1171, 1193
SfcI CTRYAG 3 cut(s) 687, 1161, 1323
SinI GGWCC 4 cut(s) 122, 503, 930, 986
SmiMI CAYNNNNRTG 3 cut(s) 117, 246, 959
SmlI CTYRAG 2 cut(s) 190, 756
SmoI CTYRAG 2 cut(s) 190, 756
SphI GCATGC 1 cut(s) 1303
Sse9I AATT 9 cut(s) 160, 172, 261, 361, 441, 918, 1094, 1203, 1405
SspMI CTAG 4 cut(s) 237, 582, 1041, 1230
SstI GAGCTC 1 cut(s) 1222
StyD4I CCNGG 3 cut(s) 350, 703, 994
StyI CCWWGG 3 cut(s) 741, 1019, 1229
TaaI ACNGT 4 cut(s) 643, 860, 1003, 1033
TaqI TCGA 6 cut(s) 147, 816, 864, 1054, 1224, 1264
TasI AATT 9 cut(s) 160, 172, 261, 361, 441, 918, 1094, 1203, 1405
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 2 cut(s) 391, 473
Tru1I TTAA 8 cut(s) 81, 246, 591, 945, 1007, 1341, 1440, 1457
Tru9I TTAA 8 cut(s) 81, 246, 591, 945, 1007, 1341, 1440, 1457
TscAI CASTG 3 cut(s) 646, 1162, 1195
TseFI GTSAC 2 cut(s) 647, 854
TseI GCWGC 9 cut(s) 257, 281, 340, 686, 770, 833, 845, 971, 1160
Tsp45I GTSAC 2 cut(s) 647, 854
TspDTI ATGAA 3 cut(s) 507, 545, 1296
TspRI CASTG 3 cut(s) 646, 1162, 1195
Tth111I GACNNNGTC 1 cut(s) 860
VpaK11BI GGWCC 4 cut(s) 122, 503, 930, 986
VspI ATTAAT 1 cut(s) 246
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 3 cut(s) 160, 361, 1203
XceI RCATGY 2 cut(s) 1303, 1427
XmaJI CCTAGG 1 cut(s) 1229
XspI CTAG 4 cut(s) 237, 582, 1041, 1230
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.